read0043320/misc/21689174 伊藤博夫 歯科治療と感染性心内膜炎の予防． (2008 in press) 四国歯学誌 null null 20080000 read0043320/misc/21689172 Hiro-O Ito Kiyoshi Nokihara Sakiko Soutome Takafumi Ohyama Screening of peptides that inhibit bacterial binding to fibronectin using combinatorial peptide libraries INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS 1573-3149 12 3 275 281 20060900 10.1007/s10989-006-9030-7 read0043320/misc/21689173 Hiro-O. Ito Infective endocarditis and dental procedures: Evidence, pathogenesis, and prevention Journal of Medical Investigation 1343-1420 53 3-4 189 198 20060800 10.2152/jmi.53.189 16953053 2-s2.0-33750299442 read0043320/misc/21689171 E Nagata H Okayama HO Ito Semba, I M Inoue T Oho Experimental infective endocarditis induced by human supragingival dental plaque in rats EUROPEAN JOURNAL OF ORAL SCIENCES 0909-8836 113 6 499 504 20051200 10.1111/j.1600-0722.2005.00258.x read0043320/misc/21689118 Y Miwa HO Ito M Inoue T Oho Induction of immune response to Streptococcus pneumoniae by administration of oral viridans streptococci via phosphorylcholine determinant Induction of immune response to Streptococcus pneumoniae by administration of oral viridans streptococci via phosphorylcholine determinant.(共著，Miwa Y et al.) FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY FEMS Immunol. Med. Microbiol.: 0928-8244 43 3 441 448 20050300 10.1016/j.femsim.2004.11.001 read0043320/misc/21689117 H Okayama E Nagata HO Ito T Oho M Inoue Experimental abscess formation caused by human dental plaque MICROBIOLOGY AND IMMUNOLOGY 0385-5600 49 5 399 405 20050000 10.1111/j.1348-0421.2005.tb03742.x read0043320/misc/21689072 HO Ito S Soutome K Nokihara M Inoue Identification and characterization of bacterial-binding property in the type III repeat domain of fibronectin BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 0006-291X 320 2 347 353 20040700 10.1016/j.bbrc.2004.05.170 read0043320/misc/21689071 Induction of immune response to Streptococcus pneumoniae by administration of oral viridans streptococci via phosphorylcholine determinant.(共著，Miwa Y et al.) 2004 in press FEMS Immunol. Med. Microbiol. null null 20040000 10.1016/j.femsim.2004.11.001 read0043320/misc/21689114 HO Ito S Soutome M Inoue Inhibition of fibronectin binding of some bacterial cells by subtle pH increase within the physiological range JOURNAL OF MICROBIOLOGICAL METHODS 0167-7012 55 1 29 34 20031000 10.1016/S0167-7012(03)00111-8 read0043320/misc/21689073 HO Ito T Nakashima T So M Hirata M Inoue Immunodominance of conformation-dependent B-cell epitopes of protein antigens BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 0006-291X 308 4 770 776 20030900 10.1016/S0006-291X(03)01466-9 read0043320/misc/21689170 本学歯学部におけるOSCEトライアルの実施方法について(共著，水流和徳ほか) 日歯教誌 19(1): 160-169 null null 20030000 read0043320/misc/21689168 歯学自己学習支援プログラム「口腔衛生学」(小椋秀亮監修，分担執筆) 担当部分:大項目「口腔衛生学」中項目「免疫」 アルプ社 null null 20030000 read0043320/misc/21689124 歯周病罹患状況と臨床検査値および歯周病原性細菌に対する血清抗体価の変化とその関連 ―同じ被験者を対象とした3年間の調査結果―(共著，石川久美子ほか) 日歯周誌 45(4): 394-401 null null 20030000 10.2329/perio.45.394 read0043320/misc/21689116 歯周病罹患状況と臨床検査値および歯周病原性細菌に対する血清抗体価の変化とその関連 ―同じ被験者を対象とした3年間の調査結果―(共著，石川久美子ほか) 日歯周誌 45(4): 394-401 null null 20030000 10.2329/perio.45.394 read0043320/misc/21689115 本学歯学部におけるOSCEトライアルの実施方法について(共著，水流和徳ほか) 日歯教誌 19(1): 160-169 null null 20030000 read0043320/misc/21689113 歯学自己学習支援プログラム「口腔衛生学」(小椋秀亮監修，分担執筆)担当部分:大項目「口腔衛生学」中項目「免疫」 アルプ社 null null 20030000 read0043320/misc/21689112 新予防歯科学(第3版) (米満正美ほか編，分担執筆) 担当部分:第4章 齲蝕とその予防 医歯薬出版社 (単行本，2003) p. 61-88 null null 20030000 read0043320/misc/21689135 牛乳とむし歯(う触)予防の関係(2)―感染症としてのう触に対する受動ワクチン― ミルクサイエンス 51 null null 20020000 read0043320/misc/21689134 牛乳とむし歯(う触)予防の関係(1)カゼインホスホペプチドのう触抑制作用 ミルクサイエンス 51, 73-75 null null 20020000 read0043320/misc/21689165 Y Furuichi HO Ito Y Izumi T Matsuyama Y Yotsumoto Y Mishige M Kojima K Yamashita M Inoue Periodontal status and serum antibody titers for Porphyromonas gingivalis fimbriae in a rural population in Japan Periodontal status and serum antibody titers for Prophyromonas gingivalis fimbriae in a rural population in Japan.(共著) JOURNAL OF CLINICAL PERIODONTOLOGY Journal of Clinical Periodontology 0303-6979 28 3 264 269 20010300 read0043320/misc/21689167 Contribution of conformational stability of hen lysozyme to induction of type 2 T-helper immune responses Immunology 104, 259-268 null null 20010000 10.1046/j.1365-2567.2001.01314.x read0043320/misc/21689166 Porphyromonas gingivalis-specific IgG subclass antibody levels as immunological risk indicators of periodontal bone loss.(共著) Journal of Clinical Periodontology 28 (9): 853-859 null null 20010000 read0043320/misc/21689111 Contribution of conformational stability of hen lysozyme to induction of type 2 T-helper immune responses Immunology 104, 259-268 null null 20010000 10.1046/j.1365-2567.2001.01314.x read0043320/misc/21689110 Immunodominant region of Actinobacillus actinomycetemcomitans 40-kilodalton heat shock protein in patients with rheumatoid arthritis.(共著) J Dent Res 80 345 50 20010000 read0043320/misc/21689076 Periodontal status and serum antibody titers for ┣DB Prophyromonas gingivalis (/)-┫DB fimbriae in a rural population in Japan.(共著) Journal of Clinical Periodontology 28 264 269 20010000 read0043320/misc/21689075 Porphyromonas gingivalis-specific IgG subclass antibody levels as immunological risk indicators of periodontal bone loss.(共著) Journal of Clinical Periodontology 28(9): 853-859 null null 20010000 read0043320/misc/21689164 Immunodominant region of Actinobacillus actinomycetemcomitans 40-kilodalton heat shock protein in patients with rheumatoid arthritis.(共著) J Dent Res 80 345 50 20000000 read0043320/misc/21689163 Effect of denture application on senile patients : relationship between mastication and immunological function. (共著) Dentistry in Japan 36 54 58 20000000 read0043320/misc/21689133 臨床現場でのう触予防の実践―リスク評価と予防の実際 歯科医療 13, 5-18 null null 20000000 read0043320/misc/21689121 OKADA Y KITADA K TAKAGAKI M ITO H‐O INOUE M Endocardiac infectivity and binding to extracellular matrix proteins of oral abiotrophia species. (共著) FEMS Immunol Med Microbiol 0928-8244 27 3 257 261 20000000 10.1016/S0928-8244(99)00193-5 10683471 read0043320/misc/21689109 Effect of denture application on senile patients : relationship between mastication and immunological function. (共著) Dentistry in Japan 36 54 58 20000000 read0043320/misc/21689074 Endocardiac infectivity and binding to extracellular matrix proteins of oral abiotrophia species. (共著) FEMS Immunol Med Microbiol 0928-8244 27 3 257 261 20000000 10.1016/S0928-8244(99)00193-5 10683471 read0043320/misc/21689161 T So SO T HO Ito ITO H‐O M Hirata HIRATA M T Ueda UEDA T T Imoto IMOTO T Extended blood half-life of monomethoxypolyethylene glycol-conjugated hen lysozyme is a key parameter controlling immunological tolerogenicity Extended blood half-life of monomethoxypolyethylene glycolconjugated hen lysozyme is a key parameter controlling immunological tolerogenicity. (共著) CELLULAR AND MOLECULAR LIFE SCIENCES Cellular and Molecular Life Sciences 1420-682X 55 8-9 1187 1194 19990700 10.1007/s000180050365 10442096 read0043320/misc/21689107 T So HO Ito M Hirata T Ueda T Imoto Extended blood half-life of monomethoxypolyethylene glycol-conjugated hen lysozyme is a key parameter controlling immunological tolerogenicity CELLULAR AND MOLECULAR LIFE SCIENCES 1420-682X 55 8-9 1187 1194 19990700 10.1007/s000180050365 10442096 read0043320/misc/21689162 Antigen recognition and the Specificity of immune system The Annals of Kagoshima University Dental School 19 23 33 19990000 read0043320/misc/21689160 SO T ITO H TSUJIHATA Y HIRATA M UEDA T IMOTO T The molecular weight ratio of monomethoxypolyethylene glycol (mPEG) to protein determines the immunotolerogenicity of mPEG proteins. (共著) Protein Engeneering 0269-2139 12 8 701 705 19990000 10.1093/protein/12.8.701 10469832 read0043320/misc/21689159 Nasopharyngeal-associated lymphoreticular tissue(NALT) immunity : fimbriae-specific Th1 and Th2 cell-regulated IgA responses for the inhibition of bacterial attachment to epithelial cells and subsequent inflammatory cytokine production「共著」 J. Immunology 162 3559 3565 19990000 read0043320/misc/21689132 免疫系の抗原認識と特異性 鹿児島大学歯学部紀要 19 23 33 19990000 read0043320/misc/21689108 Antigen recognition and the Specificity of immune system The Annals of Kagoshima University Dental School 19 23 33 19990000 read0043320/misc/21689106 The molecular weight ratio of monomethoxypolyethylene glycol (mPEG) to protein determines the immunotolerogenicity of mPEG proteins. (共著) Protein Engeneering 0269-2139 12 8 701 705 19990000 10.1093/protein/12.8.701 10469832 read0043320/misc/21689105 Nasopharyngeal-associated lymphoreticular tissue(NALT) immunity : fimbriae-specific Th1 and Th2 cell-regulated IgA responses for the inhibition of bacterial attachment to epithelial cells and subsequent inflammatory cytokine production「共著」 J. Immunology 162 3559 3565 19990000 read0043320/misc/21689103 HO Ito T So M Hirata T Koga T Ueda T Imoto Tolerogenic activity of polyethylene glycol-conjugated lysozyme distinct from that of the native counterpart IMMUNOLOGY 0019-2805 93 2 200 207 19980200 10.1046/j.1365-2567.1998.00414.x 9616369 read0043320/misc/21689156 A novel approach for detecting an immunodominant antigen of Porphyromonas gingivalis intended for diagnosis of abult periodontitis. (共著, Kawai, T. 他) Clinical & Experimental Laboratory Immunology 5 11 17 19980000 read0043320/misc/21689077 A novel approach for detecting an immunodominant antigen of Porphyromonas gingivalis intended for diagnosis of abult periodontitis. (共著, Kawai, T. 他) Clinical & Experimental Laboratory Immunology 5 11 17 19980000 read0043320/misc/21689100 HO Ito T So T Ueda T Imoto T Koga Prevention of collagen-induced arthritis (CIA) by treatment with polyethylene glycol-conjugated type II collagen; Distinct tolerogenic property of the conjugated collagen from the native one CLINICAL AND EXPERIMENTAL IMMUNOLOGY 0009-9104 108 2 213 219 19970500 read0043320/misc/21689099 HO Ito M Hirata T Koga Hen egg-white lysozyme inhibits biological activities of lipopolysaccharides from periodontopathic bacteria JOURNAL OF PERIODONTAL RESEARCH 0022-3484 32 3 295 299 19970400 read0043320/misc/21689158 SO T ITO H KOGA T WATANABE S UEDA T IMOTO T Depression of T-cell-epitope generation by stabilizing hen lysozyme. (共著, So, T. 他) Journal of Biological Chemistry 0021-9258 272 32136 32140 19970000 10.1074/jbc.272.51.32136 9405412 read0043320/misc/21689155 Role of the charged tail on localization of a surface protein antigen of Sreptococcus mutans. Infection & Immunity (Academic Journal,1997) 65 4 1531 1535 19970000 read0043320/misc/21689104 Depression of T-cell-epitope generation by stabilizing hen lysozyme. (共著, So, T. 他) Journal of Biological Chemistry 0021-9258 272 32136 32140 19970000 10.1074/jbc.272.51.32136 9405412 read0043320/misc/21689101 Role of the charged tail on localization of a surface protein antigen of Sreptococcus mutans. (共著, Murakami, Y et al. ) Infection & Immunity 65 4 1531 1535 19970000 read0043320/misc/21689098 HO Ito T Ueda Y Hashimoto T Imoto T Koga Quaternary structure-dependent idiotope and antigen binding of a monoclonal antibody specific for conformational epitope on type II collagen CELLULAR AND MOLECULAR LIFE SCIENCES 1420-682X 53 1 51 60 19970100 10.1007/PL00000579 read0043320/misc/21689095 HO Ito T Shuto H Takada T Koga Y Aida M Hirata T Koga Lipopolysaccharides from Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans promote osteoclastic differentiation in vitro ARCHIVES OF ORAL BIOLOGY 0003-9969 41 5 439 444 19960500 10.1016/0003-9969(96)00002-7 read0043320/misc/21689152 Takanori So Hiro-O Ito Toshitaka Koga Tadashi Ueda Taiji Imoto Reduced immunogenicity of monomethoxypolyethylene glycol-modified lysozyme for activation of T cells Reduced immunogenicity of monomethoxypolyethylene glycol-modified lysozyme for activation of T cells(共著, So, T. etal. ) Elsevier B.V. Immunology Letters 0165-2478 49 1-2 91 97 19960000 10.1016/0165-2478(95)02488-3 8964616 2-s2.0-0029876586 read0043320/misc/21689131 タンパク抗原の安定化によってT細胞刺能は低下する(共著 宗孝紀 他) 生化学 68 7 856 null 19960000 read0043320/misc/21689102 (Depression of T-cell stimulating copacity of proteins by stabilization) 68 7 856 null 19960000 read0043320/misc/21689096 Takanori So Hiro-O Ito Toshitaka Koga Tadashi Ueda Taiji Imoto Reduced immunogenicity of monomethoxypolyethylene glycol-modified lysozyme for activation of T cells Elsevier B.V. Immunology Letters 0165-2478 49 1-2 91 97 19960000 10.1016/0165-2478(95)02488-3 8964616 2-s2.0-0029876586 read0043320/misc/21689153 Tolerance induction by antigens modified with polyethylene glycol Clinical Immunology 27 9 1124 1128 19950000 read0043320/misc/21689130 抗原のポリエチレングリコール修飾によるトレランス誘導(共著，井本泰治 他) 臨床免疫 27 9 1124 1128 19950000 read0043320/misc/21689097 Tolerance induction by antigens modified with polyethylene glycol Clinical Immunology 27 9 1124 1128 19950000 read0043320/misc/21689144 SAKAI Yoko 酒井 洋子 ITO Hiro-o 伊藤 博夫 OKADA Hiroshi 岡田 宏 Distribution of Serum IgG Subclass Antibodies to Porphyromonas gingivalis in Periodontitis Patients Distribution of IgG subclass antibodies to Porphyromonas gingivalis in periodontitis patients The present study was conducted to investigate the responses of serum IgG subclass antibodies against Porphyromonas gingivalis and immunodominant antigens of the organism for the different IgG subclasses. Sera were obtained from patients with adult periodontitis (AP), after assessing pocket probing depth from 6 sites per tooth and a full set of intraoral radiographs at the initial examination. Enzyme-linked immunosorbent assay for IgG subclass antibodies against whole cells of P. gingivalis strain 381 in 28 periodontitis patients and 14 periodontally healthy controls revealed that all 4 subclasses and total IgG anti-P. gingivalis levels were significantly higher in the cases (P<0.0001, by Mann-Whitney U test) than those in healthy controls. IgG subclass distribution patterns varied among the patients. Antibody levels of total IgG, IgG 1, IgG 3 and IgG 4 did not correlate with any clinical parameters. However, IgG 2 titer correlated positively with mean pocket probing depth and rate of periodontally affected teeth that exhibited more than 30% bone-loss on the radiographs. Specificities of different IgG subclasses in 25 patients and 14 control subjects were examined by Western blot analysis against sonic extracts of the organism. P. gingivalis reactive IgG 2 was found in 22/25 patients and 4/14 controls, while IgG 1 responses were found in all patients and healthy controls. An antigen displaying a smeared pattern at 50∼100 kDa was immunodominant for IgG 2. Only 3/25 of AP patients and 1/14 controls showed a positive reaction in IgG 3, 5/25 of patients and no control subject were positive for IgG 4. The significance of IgG 2 responses in the periodontitis patients suggests the importance of carbohydrate antigens of P. gingivalis in periodontal infection. 抗Porphyromonas gingivalis抗体の機能と,本菌の歯周感染における主要抗原を調べる目的で,初診時歯周炎患者および健常者の血清について, IgGサブクラス特異的モノクローナル抗体を用いて,固相免疫測定法(ELISA)による抗P. gingivalis抗体価の測定と,イムノブロット法による各IgGサブクラス抗体の対応抗原の解析を行った．凍結乾燥全菌体を抗原としたELISAの結果,患者群では全てのサブクラスにおいて抗体価の有意な上昇が認められ,大部分の患者では特定のサブクラスの抗体価が選択的に上昇していた．IgG2抗体価と各種臨床指数との間には有意な正の相関が認められた．しかし,IgG 1, IgG 3, IgG 4および総IgG抗体価については,各種臨床指数との間に,相関は認められなかった．本菌の超音波破砕上清を使用したイムノブロット法による解析においては,IgG 2サブクラスの抗体が患者に特徴的な染色像を最も頻繁に示し,IgG 1サブクラスの抗体はすべての患者および健常者において反応が認められ,IgG 3, IgG 4のサブクラスに反応が認められた者は,患者においても健常者においてもまれであった．P. gingivalis特異的IgG抗体の中でもIgG 2サブクラスにおいて顕著な特徴が認められた事から,本菌の糖抗原は歯周炎の免疫応答において重要な役割を演じているものと考えられる． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology Journal of Japanese Society for Periodontology (Academic Journal,1994) 0385-0110 36 4 871 883 19940000 10.2329/perio.36.871 read0043320/misc/21689125 SAKAI Yoko 酒井 洋子 ITO Hiro-o 伊藤 博夫 OKADA Hiroshi 岡田 宏 Distribution of Serum IgG Subclass Antibodies to Porphyromonas gingivalis in Periodontitis Patients 抗Porphyromonas gingivalis抗体のIgGサブクラスと歯周炎の病態 The present study was conducted to investigate the responses of serum IgG subclass antibodies against Porphyromonas gingivalis and immunodominant antigens of the organism for the different IgG subclasses. Sera were obtained from patients with adult periodontitis (AP), after assessing pocket probing depth from 6 sites per tooth and a full set of intraoral radiographs at the initial examination. Enzyme-linked immunosorbent assay for IgG subclass antibodies against whole cells of P. gingivalis strain 381 in 28 periodontitis patients and 14 periodontally healthy controls revealed that all 4 subclasses and total IgG anti-P. gingivalis levels were significantly higher in the cases (P<0.0001, by Mann-Whitney U test) than those in healthy controls. IgG subclass distribution patterns varied among the patients. Antibody levels of total IgG, IgG 1, IgG 3 and IgG 4 did not correlate with any clinical parameters. However, IgG 2 titer correlated positively with mean pocket probing depth and rate of periodontally affected teeth that exhibited more than 30% bone-loss on the radiographs. Specificities of different IgG subclasses in 25 patients and 14 control subjects were examined by Western blot analysis against sonic extracts of the organism. P. gingivalis reactive IgG 2 was found in 22/25 patients and 4/14 controls, while IgG 1 responses were found in all patients and healthy controls. An antigen displaying a smeared pattern at 50∼100 kDa was immunodominant for IgG 2. Only 3/25 of AP patients and 1/14 controls showed a positive reaction in IgG 3, 5/25 of patients and no control subject were positive for IgG 4. The significance of IgG 2 responses in the periodontitis patients suggests the importance of carbohydrate antigens of P. gingivalis in periodontal infection. 抗Porphyromonas gingivalis抗体の機能と,本菌の歯周感染における主要抗原を調べる目的で,初診時歯周炎患者および健常者の血清について, IgGサブクラス特異的モノクローナル抗体を用いて,固相免疫測定法(ELISA)による抗P. gingivalis抗体価の測定と,イムノブロット法による各IgGサブクラス抗体の対応抗原の解析を行った．凍結乾燥全菌体を抗原としたELISAの結果,患者群では全てのサブクラスにおいて抗体価の有意な上昇が認められ,大部分の患者では特定のサブクラスの抗体価が選択的に上昇していた．IgG2抗体価と各種臨床指数との間には有意な正の相関が認められた．しかし,IgG 1, IgG 3, IgG 4および総IgG抗体価については,各種臨床指数との間に,相関は認められなかった．本菌の超音波破砕上清を使用したイムノブロット法による解析においては,IgG 2サブクラスの抗体が患者に特徴的な染色像を最も頻繁に示し,IgG 1サブクラスの抗体はすべての患者および健常者において反応が認められ,IgG 3, IgG 4のサブクラスに反応が認められた者は,患者においても健常者においてもまれであった．P. gingivalis特異的IgG抗体の中でもIgG 2サブクラスにおいて顕著な特徴が認められた事から,本菌の糖抗原は歯周炎の免疫応答において重要な役割を演じているものと考えられる． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会誌 0385-0110 36 4 871 883 19940000 10.2329/perio.36.871 read0043320/misc/21689088 Yoko SAKAI 酒井 洋子 Hiro-o ITO 伊藤 博夫 Hiroshi OKADA 岡田 宏 Distribution of IgG subclass antibodies to Porphyromonas gingivalis in periodontitis patients 抗Porphyromonas gingivalis抗体のIgGサブクラスと歯周炎の病態 The present study was conducted to investigate the responses of serum IgG subclass antibodies against Porphyromonas gingivalis and immunodominant antigens of the organism for the different IgG subclasses. Sera were obtained from patients with adult periodontitis (AP), after assessing pocket probing depth from 6 sites per tooth and a full set of intraoral radiographs at the initial examination. Enzyme-linked immunosorbent assay for IgG subclass antibodies against whole cells of P. gingivalis strain 381 in 28 periodontitis patients and 14 periodontally healthy controls revealed that all 4 subclasses and total IgG anti-P. gingivalis levels were significantly higher in the cases (P<0.0001, by Mann-Whitney U test) than those in healthy controls. IgG subclass distribution patterns varied among the patients. Antibody levels of total IgG, IgG 1, IgG 3 and IgG 4 did not correlate with any clinical parameters. However, IgG 2 titer correlated positively with mean pocket probing depth and rate of periodontally affected teeth that exhibited more than 30% bone-loss on the radiographs. Specificities of different IgG subclasses in 25 patients and 14 control subjects were examined by Western blot analysis against sonic extracts of the organism. P. gingivalis reactive IgG 2 was found in 22/25 patients and 4/14 controls, while IgG 1 responses were found in all patients and healthy controls. An antigen displaying a smeared pattern at 50∼100 kDa was immunodominant for IgG 2. Only 3/25 of AP patients and 1/14 controls showed a positive reaction in IgG 3, 5/25 of patients and no control subject were positive for IgG 4. The significance of IgG 2 responses in the periodontitis patients suggests the importance of carbohydrate antigens of P. gingivalis in periodontal infection. 抗Porphyromonas gingivalis抗体の機能と,本菌の歯周感染における主要抗原を調べる目的で,初診時歯周炎患者および健常者の血清について, IgGサブクラス特異的モノクローナル抗体を用いて,固相免疫測定法(ELISA)による抗P. gingivalis抗体価の測定と,イムノブロット法による各IgGサブクラス抗体の対応抗原の解析を行った．凍結乾燥全菌体を抗原としたELISAの結果,患者群では全てのサブクラスにおいて抗体価の有意な上昇が認められ,大部分の患者では特定のサブクラスの抗体価が選択的に上昇していた．IgG2抗体価と各種臨床指数との間には有意な正の相関が認められた．しかし,IgG 1, IgG 3, IgG 4および総IgG抗体価については,各種臨床指数との間に,相関は認められなかった．本菌の超音波破砕上清を使用したイムノブロット法による解析においては,IgG 2サブクラスの抗体が患者に特徴的な染色像を最も頻繁に示し,IgG 1サブクラスの抗体はすべての患者および健常者において反応が認められ,IgG 3, IgG 4のサブクラスに反応が認められた者は,患者においても健常者においてもまれであった．P. gingivalis特異的IgG抗体の中でもIgG 2サブクラスにおいて顕著な特徴が認められた事から,本菌の糖抗原は歯周炎の免疫応答において重要な役割を演じているものと考えられる． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of Japanese Society for Periodontology 日本歯周病学会会誌 0385-0110 36 4 871 883 19940000 10.2329/perio.36.871 read0043320/misc/21689150 Significance of IgG2 antibody against Porphyromonas gingivalis in adult periodontitis Journal of Dental Research 71 700 null 19920000 read0043320/misc/21689149 Anti-idiotypic antibody reacting to anti-Porphyromonas gingivalis antibody in sera from patients suffered from periodontitis Journal of Japanese Association for Periodontology 34 88 null 19920000 read0043320/misc/21689148 IgG subclass of serum antibodies against Porphyromonas gingivalis and their correlation to severity of adult periodontitis Journal of Dental Research 71 1047 null 19920000 read0043320/misc/21689129 イディオタイプネットワークによる歯周炎の免疫調節機構:血清抗Porphyromonas gingivalis抗体に対する抗イディオタイプ抗体の解析 日本歯周病学会誌 34 88 null 19920000 read0043320/misc/21689094 Significance of IgG2 antibody against Porphyromonas gingivalis in adult periodontitis Journal of Dental Research 71 700 null 19920000 read0043320/misc/21689093 Anti-idiotypic antibody reacting to anti-Porphyromonas gingivalis antibody in sera from patients suffered from periodontitis Journal of Japanese Association for Periodontology 34 88 null 19920000 read0043320/misc/21689092 IgG subclass of serum antibodies against Porphyromonas gingivalis and their correlation to severity of adult periodontitis Journal of Dental Research 71 1047 null 19920000 read0043320/misc/21689143 Periodontal disease from immunological aspects : T cell function of patients J. Dental Medicine(Jpn. ) 31 6 806 815 19900000 read0043320/misc/21689137 Enhanced in vitro cytotoxicity of 1,3-bis-(2-chloroethyl)-1-nitrosourea or buthionine sulfoximine combined with a reactive oxygen-generating enzyme immunotoxin. Cancer Letters 49,169-173 null null 19900000 read0043320/misc/21689136 T cell depletion of human bone marrow using an oxidase-peroxidase enzyme immunotoxin Bone Marrow Transplantation 6,395-398 null null 19900000 read0043320/misc/21689128 伊藤博夫 免疫学からみた歯周病:歯周病患者のT細胞機能 歯科ジャーナル 31 6 806 815 19900000 read0043320/misc/21689087 Periodontal disease from immunological aspects : T cell function of patients J. Dental Medicine(Jpn. ) 31 6 806 815 19900000 read0043320/misc/21689082 T cell depletion of human bone marrow using an oxidase-peroxidase enzyme immunotoxin Bone Marrow Transplantation 6,395-398 null null 19900000 read0043320/misc/21689078 Enhanced in vitro cytotoxicity of 1,3-bis-(2-chloroethyl)-1-nitrosourea or buthionine sulfoximine combined with a reactive oxygen-generating enzyme immunotoxin. Cancer Letters 49,169-173 null null 19900000 read0043320/misc/21689147 Immunotoxins containing glucose oxidase and lactoperoxidase with tumoricidal properties : In vitro killing effectiveness in a mouse plasmacytoma cell model. Cancer Research 49 1072 1080 19890000 read0043320/misc/21689142 Polyclonal B cell activation induced by Actinomyces viscosus T14V 31 4 1072 1080 19890000 read0043320/misc/21689141 An immunotoxin system intended for bone marrow purging composed of glucose oxidase and lactoperoxidase coupled to monoclonal antibody 097 Bone Marrow Transplantation 4,519-527 null null 19890000 read0043320/misc/21689127 OKADA Toshiyuki 岡田 年以 HARADA Yasushi 原田 泰 ITO Hiroo 伊藤 博夫 NOZAKI Takenori 野崎 剛徳 EBISU Shigeyuki 恵比須 繁之 KIMURA Shigenobu 木村 重信 OKADA Hiroshi 岡田 宏 Polyclonal B Cell Activation Induced by Actinomyces viscosus T14V : Responsiveness of Human Peripheral Blood B Cells Actinomyces viscosus T14V株の示す多クローン性B細胞活性化:ヒト末梢血リンパ球の応答性 We have previously reported that the sonicate extrast from Actinomyces viscosus T 14 V (Av. sup) causes polyclonal B cell activation (PBA) in murine splenic B cells without any T cell assistance. However, there are some reports describing T cell dependency of PBA induced by A. viscosus in human peripheral blood B cells, and it is still controversial whether or not A. viscosus has the capability of exhibiting PBA without the help of T cells. In this report, we examined PBA in human peripheral blood B cells using the same Av. sup which induced T cell independent PBA in murine splenic B cells. The PBA was evaluated in terms of immunoglobulin (Ig) production by means of micro-ELISA method and cell proliferation by incorporation of ^3H-TdR after incubation of cells for 5 or 7 days with Av. sup. Human peripheral blood B cells were highly purified by the following methods; The peripheral blood was collected by venipuncture and the peripheral blood mononuclear cells were harvested using gradient centrifugations. These cells were passed through a Sephadex G-10 column, and T cells were eliminated by rosette formation with sheep red blood cells. B cells were highly purified from these T cell depleted cell populations by panning method with anti-human Ig antibody coated dishes followed by complement-dependent cytotoxisity using monoclonal antibody cocktails consisting of OKT3, OKT4 and OKT 8. These highly purified B cells could not respond to T cell mitogens such as PHA. The Av. sup did induce both cell proliferation and Ig production both in human peripheral blood mononuclear cells and peripheral blood lymphocytes, while it did not induce PBA in highly purified human peripheral blood B cells without the participation of T cells. However, reintroduction of T cells into B cell cultures restored significant PBA by Av. sup. Moreover the addition of macrophage fractions allowed further PBA by Av. sup. These results indicate that the presence of T cells may be necessary for A. viscosus-induced PBA in human peripheral blood B cells (i.e. T cell-dependent PBA). It also suggested that the interactions of immunocompetent cells influenced the A. viscosus-induced PBA in human peripheral blood B cells. Actinomyces viscosus T 14 V株の超音波破砕上清(Av. sup)は,マウス脾細胞に対してはT細胞非依存性に多クローン性B細胞活性化(PBA)作用を有することが明らかとなっている．本実験では,Av. supのPBA作用についてヒト末梢血リンパ球(PBL)を対象に,特にそのT細胞依存性に関して検討を行った．その結果Av. supは,十分に精製したヒトPBL B細胞単独群に対しては細胞分裂や免疫グロブリン(Ig)産生を誘導できなかった．しかし,これにヒトPBL T細胞を添加することで細胞分裂およびIg産生の誘導が観察されたことから,ヒトPBLに対してはAv. supの示すPBA作用がT細胞依存性に発現されることが明らかとなった．またさらに,この系に単球-マクロファージ系細胞を添加することによってAv. supの示すPBA作用が増強されることから,ヒト末梢血B細胞に対するAv. supのPBA作用には,各種の免疫担当細胞間の相互作用が深く関連していることが示唆された． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会誌 0385-0110 31 4 1072 1080 19890000 10.2329/perio.31.1072 read0043320/misc/21689091 Immunotoxins containing glucose oxidase and lactoperoxidase with tumoricidal properties : In vitro killing effectiveness in a mouse plasmacytoma cell model. Cancer Research 49 1072 1080 19890000 read0043320/misc/21689086 Polyclonal B cell activation induced by Actinomyces viscosus T14V 31 4 1072 1080 19890000 read0043320/misc/21689085 An immunotoxin system intended for bone marrow purging composed of glucose oxidase and lactoperoxidase coupled to monoclonal antibody 097 Bone Marrow Transplantation 4,519-527 null null 19890000 read0043320/misc/21689081 Toshiyuki OKADA 岡田 年以 Yasushi HARADA 原田 泰 Hiroo ITO 伊藤 博夫 Takenori NOZAKI 野崎 剛徳 Shigeyuki EBISU 恵比須 繁之 Shigenobu KIMURA 木村 重信 Hiroshi OKADA 岡田 宏 Actinomyces viscosus T14VB:(in Japanese) Actinomyces viscosus T14V株の示す多クローン性B細胞活性化 : ヒト末梢血リンパ球の応答性 We have previously reported that the sonicate extrast from Actinomyces viscosus T 14 V (Av. sup) causes polyclonal B cell activation (PBA) in murine splenic B cells without any T cell assistance. However, there are some reports describing T cell dependency of PBA induced by A. viscosus in human peripheral blood B cells, and it is still controversial whether or not A. viscosus has the capability of exhibiting PBA without the help of T cells. In this report, we examined PBA in human peripheral blood B cells using the same Av. sup which induced T cell independent PBA in murine splenic B cells. The PBA was evaluated in terms of immunoglobulin (Ig) production by means of micro-ELISA method and cell proliferation by incorporation of ^3H-TdR after incubation of cells for 5 or 7 days with Av. sup. Human peripheral blood B cells were highly purified by the following methods; The peripheral blood was collected by venipuncture and the peripheral blood mononuclear cells were harvested using gradient centrifugations. These cells were passed through a Sephadex G-10 column, and T cells were eliminated by rosette formation with sheep red blood cells. B cells were highly purified from these T cell depleted cell populations by panning method with anti-human Ig antibody coated dishes followed by complement-dependent cytotoxisity using monoclonal antibody cocktails consisting of OKT3, OKT4 and OKT 8. These highly purified B cells could not respond to T cell mitogens such as PHA. The Av. sup did induce both cell proliferation and Ig production both in human peripheral blood mononuclear cells and peripheral blood lymphocytes, while it did not induce PBA in highly purified human peripheral blood B cells without the participation of T cells. However, reintroduction of T cells into B cell cultures restored significant PBA by Av. sup. Moreover the addition of macrophage fractions allowed further PBA by Av. sup. These results indicate that the presence of T cells may be necessary for A. viscosus-induced PBA in human peripheral blood B cells (i.e. T cell-dependent PBA). It also suggested that the interactions of immunocompetent cells influenced the A. viscosus-induced PBA in human peripheral blood B cells. Actinomyces viscosus T 14 V株の超音波破砕上清(Av. sup)は,マウス脾細胞に対してはT細胞非依存性に多クローン性B細胞活性化(PBA)作用を有することが明らかとなっている．本実験では,Av. supのPBA作用についてヒト末梢血リンパ球(PBL)を対象に,特にそのT細胞依存性に関して検討を行った．その結果Av. supは,十分に精製したヒトPBL B細胞単独群に対しては細胞分裂や免疫グロブリン(Ig)産生を誘導できなかった．しかし,これにヒトPBL T細胞を添加することで細胞分裂およびIg産生の誘導が観察されたことから,ヒトPBLに対してはAv. supの示すPBA作用がT細胞依存性に発現されることが明らかとなった．またさらに,この系に単球-マクロファージ系細胞を添加することによってAv. supの示すPBA作用が増強されることから,ヒト末梢血B細胞に対するAv. supのPBA作用には,各種の免疫担当細胞間の相互作用が深く関連していることが示唆された． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 日本歯周病学会会誌 0385-0110 31 4 1072 1080 19890000 10.2329/perio.31.1072 read0043320/misc/21689146 The function of gingival lymphocytes on the establishment of human periodontitis. Advances in Dental Research 2 2 364 367 19880000 read0043320/misc/21689140 Polyclonal induction of IgG antibody forming cells by stimulation with Actinomyces viscosus T14V. J. Periodontal Research 23,122-126 null null 19880000 read0043320/misc/21689138 Possible role of T-cells in the establishment of IgG plasma cell-rich periodontal lesion ;Augmentation of IgG synthesis in the polyclonal B-cell activation response by autoreactive T-cells. J. Periodontal Research 23,39-45 null null 19880000 read0043320/misc/21689090 The function of gingival lymphocytes on the establishment of human periodontitis. Advances in Dental Research 2 2 364 367 19880000 read0043320/misc/21689089 T-cell requirement for establishment of the IgG dominant B-cell lesion in periodontitis. Journal of Periodontal Research 22 187 189 19880000 read0043320/misc/21689083 Possible role of T-cells in the establishment of IgG plasma cell-rich periodontal lesion ; Augmentation of IgG synthesis in the polyclonal B-cell activation response by autoreactive T-cells. J. Periodontal Research 23,39-45 null null 19880000 read0043320/misc/21689079 Polyclonal induction of IgG antibody forming cells by stimulation with Actinomyces viscosus T14V. J. Periodontal Research 23,122-126 null null 19880000 read0043320/misc/21689145 Hiroshi Okada Hiroo Ito Yasushi Harada T‐cell requirement for establishment of the IgG‐dominant B‐cell lesion in periodontitis Journal of Periodontal Research 1600-0765 22 3 187 189 19870000 10.1111/j.1600-0765.1987.tb01564.x 2955095 2-s2.0-0023335176 read0043320/misc/21689139 伊藤 博夫 T-cells modulate T-independent polyclonal B cell activation and enhance IgG synthesis J. Jpn. Sci. Periodontal 0385-0110 29 1 22 36 19870000 10.2329/perio.29.22 3509823 read0043320/misc/21689126 ITO Hiroo 伊藤 博夫 T Cells Modulate T-independent Polyclonal B Cell Activation and Enhance IgG Synthesis T細胞による多クローン性B細胞活性化の修飾:特に,多クローン性IgG産生の増強について. Using murine splenic B and T cell populations, it was investigated in vitro how T cells effected on T-independent polyclonal B cell activation (PBA) induced by sonic extract from Actinomyces viscosus T14V and bacterial lipopolysaccharide. By admixture of separated B and T cell populations, it was demonstrated that normal splenic T cells were able to enhance lgG synthesis in PBA. Further experiments were done to investigate the phenotype of the T cells and the mechanisms to enhance IgG synthesis in PBA. Results were as follows ; 1. L3T4 positive T cells were able to enhance IgG synthesis in PBA, but Lyt-2 postitive cells were not. 2. L3T4 positive T cells were activated in PBA, but not activated by polyclonal B cell activators themselves. 3. Anti-L3T4 monoclonal antibody blocked the T cell function to enhance IgG synthesis. 4. Ia antigens were increased on the surface of B cells activated in PBA. These B cells strongly activated T cells in syngeneic mixed lymphocyte reaction. These results suggested that autoreactive T cells which recognized self-Ia antigens expressed on the surface of B cells would contribute to augmentation of IgG synthesis. Therefore, it was supposed that periodontal lesion dominated by IgG producing cells could be established by PBA modulated by autoreactive T cells. Actinomyces viscosus T14V株の超音波破砕上液 (Av. sup) と大腸菌リポ多糖 (LPS) によるT細胞非依存性の多クローン性B細胞活性化 (PBA) に対するT細胞の影響を検討した．その結果, T細胞はT細胞非依存性のPBAによるIgG産生を増強する事が明らかになった．更に, この様な機能を有するT細胞の性状, 及び, T細胞によるIgG産生の増強機構について検討した結果, helper/inducerサブポピュレーションのT細胞が, PBAによって活性化された自己のB細胞表面上のIa抗原に反応して活性化され, この様な自己反応性T細胞がPBAによるIgG産生を増強する事が示唆された．以上の結果から, PBAに自己反応性T細胞が関与して, 多数のIgG産生形質細胞が浸潤, 増生している歯周炎病巣が形成される可能性が示唆される． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会誌 0385-0110 29 1 22 36 19870000 10.2329/perio.29.22 3509823 read0043320/misc/21689084 Hiroo ITO T-cells modulate T-independent polyclonal B cell activation and enhance IgG synthesis J. Jpn. Sci. Periodontal 0385-0110 29 1 22 36 19870000 10.2329/perio.29.22 3509823 read0043320/misc/21689080 Hiroo ITO T細胞による多クローン性B細胞活性化の修飾:特に,多クローン性IgG産生の増強について(in Japanese) 日本歯周病学会誌 0385-0110 29 1 22 36 19870000 10.2329/perio.29.22 3509823 read0043320/books_etc/5460540 歯周病と全身の健康を考える(共著, 長田恵美;長谷川紘司ほか編, 分担執筆, 担当部分:急性歯性感染症の原発病巣としての歯周ポケット) 財)ライオン歯科衛生研究所，東京 20040000 read0043320/books_etc/5460539 実践予防歯科ハンドブック (竹原直道ほか編，分担執筆) 担当部分:*歯周疾患に対する薬剤の応用法 *矯正患者のメンテナンス 医歯薬出版社，東京 20040000 read0043320/books_etc/5460532 歯周病と全身の健康を考える(共著, 長田恵美;長谷川紘司ほか編, 分担執筆, 担当部分:急性歯性感染症の原発病巣としての歯周ポケット) 財)ライオン歯科衛生研究所，東京 20040000 read0043320/books_etc/5460531 実践予防歯科ハンドブック (竹原直道ほか編，分担執筆) 担当部分:*歯周疾患に対する薬剤の応用法 *矯正患者のメンテナンス 医歯薬出版社，東京 20040000 read0043320/books_etc/5460538 新予防歯科学(第3版) (米満正美ほか編，分担執筆) 担当部分:第4章 齲蝕とその予防 医歯薬出版社 20030000 read0043320/books_etc/5460537 Application of combinatorial peptide libraries, as an example for prevention against infective endocaditis. In: Yamada, T. (Ed.) Peptide Science 2002, the Japanese Peptide Society, 20030000 read0043320/books_etc/5460530 Application of combinatorial peptide libraries, as an example for prevention against infective endocaditis In: Yamada, T. (Ed.) Peptide Science 2002, the Japanese Peptide Society 20030000 read0043320/books_etc/5460536 Development of lead compounds focusing on prevention of infective endocarditis using combinatorial peptide libraries. In: Benedetti, E., Pedone, C. (Eds.) Peptides 2002 , Wiley, U.K. 20020000 read0043320/books_etc/5460529 Development of lead compounds focusing on prevention of infective endocarditis using combinatorial peptide libraries In: Benedetti, E., Pedone, C. (Eds.) Peptides 2002 , Wiley, U.K. 20020000 read0043320/books_etc/5460533 免疫学辞典(第2版)イディオタイプ(イディオタイプ決定基,イディオトープ),イディオタイプ抑制,交差反応性イディオタイプ,T15イディオタイプ,調節性イディオタイプ.分担執筆 東京化学同人社 20010000 read0043320/books_etc/5460535 口腔診断学 第3章歯周組織,iii.血液検査,2.リンパ球機能検査,下里常弘他編,分担執筆 デンタルダイヤモンド社 19920000 read0043320/books_etc/5460534 歯科臨床検査事典,β-グルクローニダーゼ活性 pH検査(歯周ポケット) リンパ球NK細胞検査,リンパ球Fcレセプター保有細胞検査細胞検査,リンパ球Suppressor,Cytotoxic cell検査 分担執筆(塩田重利監修) クインテッセンス社 19900000 read0043320/works/3307826 感染性心内膜炎予防のためのリード化合物探索 20000000 read0043320/works/3307825 歯周病ワクチン合成のための基礎的研究 20000000 read0043320/works/3307827 ホスホリルコリン抗体に関する研究 20010000 read0043320/works/3307828 牛乳ミセル性リン酸カルシウムのう蝕および骨粗鬆症予防効果 20030000 read0043320/works/3307824 奄美島嶼圏の超高齢者の口腔および全身健康状態とquality of life (QOL) に関する調査 20030000 read0043320/works/3307819 Preventive effects of micellic calcium phosphate-phosphopeptides (MCP-PP) complex on dental caries and osteopolosis. 20030000 read0043320/works/3307815 Investigation of very old people in Amami islands for oral and general health conditions and quality of life 20030000 read0043320/works/3307817 Search for lead compounds intended for prevention of infective endocarditis 20000000 read0043320/works/3307816 A Fundamental Research for synthetic vaccine for periodontal disease 20000000 read0043320/works/3307818 Immunological study for anti-phosphorylchdine antibodies in mice and humans 20010000 read0043320/works/3307823 鹿児島県民の歯周病罹患実態,保健意識,保健行動に関する調査 19980000 read0043320/works/3307814 Investigation for periodontal conditions, health consciousness, and health behavior of residents in Kagoshima prefecture. 19980000 read0043320/works/3307820 イディオタイプネットワークによる歯周炎の免疫調節機構 19900000 read0043320/works/3307811 Idiotype network regulation in periodontitis. 19900000 read0043320/works/3307822 歯周炎患者における熱ショック蛋白質に対する免疫応答 19930000 read0043320/works/3307813 Immune responses against heat-shock proteins in periodontitis 19930000 read0043320/works/3307821 酵素免疫測定法の高感度化の為の基礎的研究 19890000 read0043320/works/3307812 Improving Sensitivity of enzyme-Linked immuno-sorbent assay intended for dental clinical examination 19890000