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label: url

en: Dynamics of Salivary Gland AQP5 under Normal and Pathologic Conditions

en: International Journal of Molecular Sciences


en: Reversible Conversion among Subtypes of Salivary Gland Duct Cells as Identified by Production of a Variety of Bioactive Polypeptides

en: Four major kallikreins (mK1, mK22, mK9, and mK13) were identified in the mouse submandibular gland (SMG). mK1, a true tissue kallikrein, was used as a protein marker to identify different types of SMG granular convoluted tubule (GCT) cells along with epidermal growth factor (EGF), nerve growth factor (NGF), and renin. Kallikrein mK1 was localized in a very small number (~5%) of GCT cells, which were scattered throughout the GCT, indicating that the majority of GCT cells are mK1-negative. Among mK1-positive cells, particularly strong signals were observed in a small number of narrow cells, recognized as slender granular cells (SG cells, Type IV), in the GCT. After postnatal development of the SMG, GCT cells are no longer uniform based on the bioactive substances (mK1, EGF, NGF, and renin) that they produce and secrete. GCT cells were classified into four subtypes, Types I-IV, and it became clear that these subtypes are complicatedly and reversibly converted by the endocrine hormones 5α-dihydrotestosterone (DHT) and triiodothyronine (T). Duct segments with similar morphology or hormone dependency were recognized in the sublingual and parotid glands. The presence of duct cells with such characteristics is therefore a common feature of the three major salivary glands of rodents.

en: Acta Histochemica et Cytochemica

ja: Acta Histochemica et Cytochemica


en: Possible Existence of a Salivary Gland-Oral Mucosa/Gingiva Axis Under Challenges by Endotoxins

en: Journal of Molecular and Cellular Biology Forecast


en: Suppression of aquaporin 5 gene expression by endotoxin via cross-coupling of transcription factors (In Japanese)

ja: 転写因子クロスカップリングを介した内毒素によるアクアポリン5の発現抑制

en: Seikagaku

ja: 生化学


en: Processing of IL-1beta by tissue kallikrein in the mouse submandibular gland

ja: 顎下腺における炎症性サイトカインIL-1betaのプロセッシング

en: We have shown earlier that the expression of interleukin-1β (IL-1β) mRNA is increased in the submandibular gland (SMG) after injection of lipopolysaccharide (LPS). In this present study we suggest that tissue kallikrein mK13 is a candidate of the processing enzyme for the precursor of IL-1β (pro-IL-1β) in the SMG. High levels of 17.5- and 20-kDa IL-1β proteins were detected by Western blotting in the SMG, and saliva from LPS-injected mice. Despite this fact, pro-IL-1β with a molecular size of 35-kDa was not detected in this tissue. The protein for IL-1β-converting enzyme (ICE) was expressed only at a low level in the SMG as compared with its level in various epithelial tissues or LPS-stimulated macrophages. We detected the strong expression of members of kallikrein family (mK1, mK9, mK13, and mK22) in the SMG but not other tissues. By incubation with mK13, but not with mK1, mK9, nor mK22, the 35-kDa pro-IL-1β was cleaved to give two major products with molecular masses of 17.5- and 22- kDa, which production was inhibited by PMSF, a serine protease inhibitor, but not by ICE inhibitors. The pro-IL-β peptide segment, which includes the putative processing site, was hydrolyzed at Leu^<113> and Leu^<114> by incubation with mK13. The immunohistochemistry and an autonomic therapy experiment showed that IL-1β and kallikrein mK13 were co-localized in the secretory granules of granular convoluted tubular cells. Our present results thus suggest that kallikrein mK13 is a plausible candidate for the processing enzyme for pro-IL-1β in the SMG of mice.

en: Shikoku Dental Research

ja: 四国歯学会雑誌


en: Molecular mechanism of expression and regulation of water channel, esp. aquaporins in the exocrine gland

ja: 外分泌腺における水チャネル，特にアクアポリン5の発現と機能調節の分子機構

en: , Report of research project, grant in-aid for scientific research (B)

ja: 平成18年度-平成20年度文部省科学研究費補助金(基盤研究(B))研究成果報告書

misc_type: others