Resistin release from neutrophils is induced by Porphyromonas gingivalis lipopolysaccharide.

IADR (International Association of Dental Research) Barcelona, Spain, July 14-17, 2010


Induced expression of a subtilisin-like proprotein convertase PACE4 in the regeneration model of rat submandibular gland.

The 4th International Symposium on Salivary Glands in Honor of Niels Stensen


Sexual difference in the regeneration model of the rat submandibular gland.


Post-translational modifications of water channel aquaporin-5 in salivary gland cells, Oral Neuroscience 2016


Synaptic plasticity in the brain -Roles of NMDA receptor- (Invited lecture at Nantong University)


The Defense System of Oral Cavity: Lipopolysaccharide Induced Inflammatory Response in the Mice Salivary Gland

BIT's 1st Annual International Congress of Oral & Dental Medicine, Session 9: Oral Immunological Diseases, Allergy and Inflammation


Salivary Gland Development, Differentiation, and Regeneration - Role of Subtilisin-like Proprotein Convertase PACE4/SPC4

BIT's 1st Annual International Congress of Oral & Dental Medicine, Session 3: Oral Biology


Effects of natural point mutation of rat aquaporin 5 expressed in vitro on its capacity of water permeability and membrane trafficking

The Journal of Medical Investigation : JMI

In the colony of Sprague-Dawley (SD) strain, we found that there were rats expressing a mutant AQP5, which has a point mutation at nt 308 (G308A), leading to a replacement of (103)Gly with (103)Asp in the 3rd transmembrane domain. The mutant molecule scarcely expressed in the acinar cells, probably because of ineffective trafficking. The mutant molecule, however, showed normal water permeability when assessed by the oocyte system.


Induction of calprotectin mRNAs by lipopolysaccharide in the salivary gland of mice

Calprotectin is a major cytosolic calcium-binding protein of leukocytes which belongs to the S100 protein family. S100A8 and S100A9, major types of calprotectin are heterodimeric complexes being composed of light- and heavy-chain subunits. The calprotectin levels in the plasma, feces, synovial fluid, gingival crevicular fluid, dental calculus and saliva change when the host animal suffers from several inflammatory diseases. Members of Toll-like receptor (TLR) family are pattern-recognition receptors for lipopolysaccharide (LPS) and other pathogens. Here we examined if the biological role of TLR receptor is reflected to the calprotectin expression in the salivary gland. Time course study by using real-time RT-PCR detected higher levels of S100A8 and S100A9 mRNA at 1.5-3 h after injection of LPS in both the submandibular gland (SMG) and parotid gland (PG) of C3H/HeN mice but not in the same tissues of C3H/HeJ, a TLR-4 mutant strain, indicating that this induction is mediated via the TLR-4. These results indicate that, an inflammatory marker, calprotectin, is expressed in the mouse salivary gland and that LPS stimulated its synthesis. Calprotectin (S100A8/A9) showed minimum expression in all cellular segments in the SMG except excretory duct cells, which showed strong signal at the cytoplasm. LPS induced their expressions in the granular convoluted tubular cells and striated duct cells. In the PG, these proteins were expressed very weakly in both duct and acinar cells with a little stronger staining for the former cells. LPS injection induced calprotectin (S100A8/A9) in both duct and acinar cells especially in the former cells.


Down-regulation of submandibular gland AQP5 following parasympathetic denervation in rats

Following chorda tympani denervation (CTD, parasympathetomy), the protein levels of aquaporin5 (AQP5) as well as AQP1 and Na(+)K(+)ATPase alpha-subunit in the rat submandibular gland (SMG) were found to be decreased significantly. However, the level of another membrane protein, dipeptidyl peptidase IV was not affected by CTD, suggesting a selective reduction of AQP5, AQP1, and Na(+)K(+)ATPase alpha -subunit proteins by CTD. However, the AQP5 mRNA level was scarcely affected by CTD, which suggested that transcription process of AQP5 was unaffected by this operation. AQP5 protein was shown to be degraded in vitro by the extract of the SMG obtained from normal rat; inhibitor experiments in vitro suggested cathepsin B was a responsible enzyme. Co-localization of AQP5 and LAMP-2, a lysosomal marker, implicated AQP5 is degraded in lysosomes. A significant increase in the protein levels of LC3-II, an autophagy marker, at day 1 after CTD, and co-localization of the LC3 protein and AQP5, suggested that CTD activated autophagy of SMG, leading to AQP5 degradation.


Involvement of the IL-6/STAT3/Sca-1 system in proliferation of duct cells following duct ligation in the submandibular gland of mice

Ligation of the main excretory duct (MED) of the mouse submandibular gland (SMG) induced the expression of Sca-1, a stem cell marker. Sca-1 expression increased prominently in almost all of cells in the duct system, except the acinar cells. Sca-1 induction was accompanied with phosphorylated-STAT3 (Y705) elevation, which was localized in the nuclei of all duct cells. Electrophoretic mobility shift assay (EMSA) confirmed the specific binding of STAT3 to the GAS sequence, a biding site of gamma interferon activating site. Present study suggested one of the initial steps of the tissue regeneration after injury includes STAT3 pathway.


Salivary Gland Development: Its mediation by a subtilisin-like proprotein convertase, PACE4

The submandibular gland (SMG) develops under the epithelial-mesenchymal interaction. Its process is regulated by various growth/differentiation factors, which are synthesized as inactive precursors and activated via the limited proteolysis at their multi basic amino acid site(s) such as Arg-X-Lys/Arg-Arg. Although many of these processing steps are elucidated to be catalyzed by subtilisin-like proprotein convertases (SPCs), little is known about the role of SPCs in the SMG development. Here, we focused upon the physiological role of PACE4 (SPC4), a member of SPC family, in the SMG development. In the organ culture system of rat embryonic SMG (E15), Dec-RVKR-CMK, a potent inhibitor for SPCs, inhibited the salivary branching and the expression of an exocrine gland type water channel, AQP5. However, other peptidyl-CMKs and inhibitors for trypsin-like serine proteases including leupeptin did not affect the salivary branching and AQP5 expression. Dec-RVKR-CMK also suppressed the expression of PACE4, but not furin, another member of the family. The specific antibody for the catalytic domain of PACE4 suppressed the salivary branching and AQP5 expression similarly. These inhibitory effects of Dec-RVKR-CMK were partially rescued by the addition of recombinant BMP2 whose precursor is a candidate for the physiological substrates of PACE4. Further, the transcriptional silencing of PACE4 by its specific siRNAs caused the suppression of both the salivary branching and AQP5 expression in the present organ culture system. These observations strongly support the idea that PACE4 mediates the SMG development.


Potential down-regulation of parotid gland AQP5 by LPS via cross-coupling of NF-κB/AP1

The 11th International Symposium on Exocrine Secretion, Tokushima 09 ''Exocrine Secretion Mechanism and Disease''


Is AQP5 down-regulated via autophagic pathway following chorda tympani denervation?

The International Symposium on Oral Sciences to Improve the Quality of Life, organized by Toshihiko Nagata


Analyses of rat AQP5 G103D mutant expressed in MDCK-II cells and Xenopus oocytes


Activation of IL-6/STAT3/Sca-1 system induces proliferation of duct cells in the duct-ligated mouse submandibular gland

The 2nd International Symposium on The Future Direction of Oral Sciences in The 21st Century-Oral Sciences for Our Healthy Life-, organized by Toshihiko Nagata


A naturally occurring point mutation in rat AQP5 influencing its membrane trafficking in and water secretion from salivary gland

The 5th International Conference of Aquaporin


Point mutation of AQP5 in Sprague-Dawley rats and its implication for salivary gland physiology

The 1st International Symposium and Workshop on The Future Direction of Oral Sciences in The 21st Century, organized by Bando, E.


Molecular and cellular analyses of mutant AQP5 which occurred naturally in Spraque - Dawley rats

3rd International Symposium on Salivary Glands in Honor of Niels Stensen


食品成分が唾液腺機能に及ぼす影響

食品成分が唾液腺機能に及ぼす影響


周辺環境が主観的感覚および摂食行動に及ぼす影響:筋電図を用いた研究

周辺環境が主観的感覚および摂食行動に及ぼす影響:筋電図を用いた研究

日本味と匂学会第55回大会

日本味と匂学会第55回大会


Relationship between brain activities and feeding behavior under comfortable and uncomfortable environments.

快・不快環境下における脳活動と摂食行動の関連性

第55回日本味と匂い学会

第55回日本味と匂い学会


Localization of subtilisin-like proprotein convertase PACE4 in the course of development of the rat submandibular gland

サチライシン様前駆体蛋白質変換酵素PACE4のラット顎下腺発生過程における局在

第62回歯科基礎医学会学術大会

第62回歯科基礎医学会学術大会


Localization of subtilisin-like proprotein convertase PACE4 during development of the rat salivary gland

ラット唾液腺発生過程におけるサチライシン様前駆体蛋白質変換酵素PACE4の局在

第93回日本生化学会大会

第93回日本生化学会大会


Increase in theta-band EEG activities under tasting chocolate with unmatched odor stimulation

第41回日本神経科学大会

第41回日本神経科学大会


Theta band brain activity during checking the unmatched olfactory-taste information

匂いと味の不一致が引き起こすシータ波領域脳活動


甘味とニオイのミスマッチが甘味認知に与える影響

甘味とニオイのミスマッチが甘味認知に与える影響

第69回日本生理学会中国・四国地方会

第69回日本生理学会中国・四国地方会


唾液腺再生モデルにおける雌雄差の影響

唾液腺再生モデルにおける雌雄差の影響

第69回日本生理学会中国・四国地方会

第69回日本生理学会中国・四国地方会


Influences of Match/Mismatch of taste and odor on taste perception: An electroencephalogram frequency analysis study

味と匂いのミスマッチが味覚認知に与える影響:脳波周波数分析を用いた研究

日本味と匂学会第51回大会

日本味と匂学会第51回大会


カフェイン投与により誘発される視覚野オシレーション におけるnon-NMDA受容体とNMDA受容体の相互交錯的関与

第40回日本神経科学大会

第40回日本神経科学大会


Influences of olfactory stimulation on taste perception: An electroencephalogram frequency analysis study

The 94th Annual Meeting of the Physiological Science of Japan


Sexual difference in regeneration of salivary gland

唾液腺再生過程で見られる雌雄差について

第58回歯科基礎医学会学術大会

第58回歯科基礎医学会学術大会


Induction of a subtilisin-like proprotein convertase PACE4 in regeneration model of salivary gland Part II

唾液腺再生モデルにおけるサチライシン様前駆体蛋白質変換酵素PACE4の発現誘導-Part II-

第58回歯科基礎医学会学術大会

第58回歯科基礎医学会学術大会


Influences of oral impairment on neural oscilltion and wave propagation in the neocortex of rats

第38回日本神経科学大会

第38回日本神経科学大会


Expression of water channel AQP5 in regeneration model of salivary gland

唾液腺再生モデルにおける水チャネルAQP5の発現

福岡国際会議場, 福岡県

福岡国際会議場, 福岡県


Enhanced ubiquitylation and downregulation of aquaporin-5 by ubiquitin ligases

ユビキチンリガーゼによるAQP5のユビキチン化亢進とダウンレギュレーション

福岡国際会議場, 福岡県

福岡国際会議場, 福岡県


Induced expression of subtilisin-like proprotein convertase in regeneration model of salivary gland

唾液腺再生モデルにおけるサチライシン様前駆体蛋白質変換酵素の発現誘導

第56回歯科基礎医学会(福岡国際会議場/福岡県)

第56回歯科基礎医学会(福岡国際会議場/福岡県)


Involvment of subtilisin-like proprotein convertase PACE4 in regeneration of salivary gland

唾液腺再生におけるサチライシン様前駆体蛋白質変換酵素PACE4の関与

岡山コンベンションセンター/岡山県

岡山コンベンションセンター/岡山県


ヒト唾液腺HSG細胞における構成的なAQP5の取り込み

ヒト唾液腺HSG細胞における構成的なAQP5の取り込み

岡山コンベンションセンター/岡山県

岡山コンベンションセンター/岡山県


唾液腺再生におけるサチライシン様前駆体蛋白質変換酵素PACE4の関与


唾液腺再生におけるサチライシン様前駆体蛋白質変換酵素PACE4の関与

岡山コンベンションセンター/岡山県

岡山コンベンションセンター/岡山県


ラット大脳皮質におけるnon-NMDA受容体活動に依存する20Hzオシレーション

国立京都国際会館/京都府

国立京都国際会館/京都府


ヒト唾液腺HSG細胞における構成的なAQP5の取り込み

ヒト唾液腺HSG細胞における構成的なAQP5の取り込み


唾液腺細胞におけるアクアポリン5の翻訳後修飾

唾液腺細胞におけるアクアポリン5の翻訳後修飾

第53回歯科基礎医学会学術大会 サテライトシンポジウムSS11

第53回歯科基礎医学会学術大会 サテライトシンポジウムSS11


唾液腺細胞におけるアクアポリン5の翻訳後修飾 --- 第53回歯科基礎医学会学術大会(2011年9月30日-10月2日)サテライトシンポジウムSS11


Calprotectin expression in the mouse salivary gland: Induction by lipopolysaccharide


唾液腺細胞におけるアクアポリン5リン酸化のシグナル伝達機構

唾液腺細胞におけるアクアポリン5リン酸化のシグナル伝達機構


アクアポリン5のユビキチン化とその細胞内膜輸送系における役割

アクアポリン5のユビキチン化とその細胞内膜輸送系における役割


Calprotectin induction by lipopolysaccharide in the salivary gland of mice

マウス唾液腺でのリポ多糖によるカルプロテクチン誘導


AQP5 degradation via autophagy following parasympathetic denervation in the rat SMG.

ラット顎下腺での副交感神経切除に続くオートファジーを介したAQP5分解


An AQP5 G103D mutant found in SD rats shows normal water permeability but reduced membrane trafficking

SDラットで見られるAQP5 G103D変異は正常な水透過性を示すが，膜へのトラフィッキングが低下した


Down regulation of submandibular gland AQP5 by chorda tympany denervation

副交感神経切除による顎下腺AQP5のダウンレギュレーション


Involvement of IL-6-STAT3-Sca-1 system in the proliferation of duct cells in the ligated mouse submandibular gland

導管結紮マウス顎下腺における導管細胞の増殖へのIL-6-STAT3-Sca-1系の関与


LPSによる耳下腺AQP5の発現制御とそのシグナル伝達経路

LPSによる耳下腺AQP5の発現制御とそのシグナル伝達経路

第59回日本生理学会中国四国地方会

第59回日本生理学会中国四国地方会


唾液腺腺房細胞の発生・分化・成熟化と水チャネルAQP5発現

唾液腺腺房細胞の発生・分化・成熟化と水チャネルAQP5発現

第59回日本生理学会中国四国地方会

第59回日本生理学会中国四国地方会


Transient increase in IL-6 by duct ligation leading to continuous elevation of Sca-1, a stem cells marker, in the mouse submandibular gland

導管結紮によるマウス顎下腺での一過性のIL-6の増加が幹細胞マーカーSca-1の持続的な上昇を引き起す

第49回歯科基礎医学会学術大会

第49回歯科基礎医学会学術大会


Analyses of function of rat mutant AQP5 expressed in MDCK II cells

MDCK II細胞で発現させたラット変異AQP5の機能解析


Regulation of submandibular gland AQP5 protein expression by lysosomal system

リソゾーム系による顎下腺AQP5蛋白質発現の調節


唾液腺腺房細胞の分化とAQP5発現

唾液腺腺房細胞の分化とAQP5発現


Functional analyses of rat mutant AQP5 expressed in vitro system and it's implication to abnormal saliva secretion

in vitroシステムで発現したラット変異AQP5の機能解析と異常唾液分泌への影響


マウス耳下腺における水チャネルAQP5のイソプロテレノールによるdouwn-regulationの機構


イソプロパノール反復投与によるマウス唾液腺機能に及ぼす影響

イソプロパノール反復投与によるマウス唾液腺機能に及ぼす影響


Induction of a subtilisin-like proprotein convertase PACE4 in regeneration model of salivary gland Part II


Sexual difference in regeneration of salivary gland

唾液腺再生過程で見られる雌雄差について


唾液腺再生への subtilisin-like proprotein convertase PACE4の関与

自然科学研究機構生理学研究所研究会「唾液腺形態形成研究会~機能解析から器官再生へ~」

自然科学研究機構生理学研究所研究会

自然科学研究機構生理学研究所研究会


細胞内cAMP上昇が大脳皮質味覚野から口腔体性感覚野への信号伝播速度に与える影響