read0051714/published_papers/54665302 Misaki Ichinomiya Yasuhiko Shirayama Shinji Fujiwara Mio Kitamura Shino Suma Kataoka K. Comparison of Social Frailty and Oral Health Literacy among Older Adults in Urban and Rural Areas: A Cross-Sectional Study Clin Interv Aging 21 620565 null 20260400 10.2147/CIA.S620565 read0051714/published_papers/49401330 Yutaka Kusumoto Mizuki Ueda Mayuko Hashimoto Haruka Takeuchi Naoko Okada Junya Yamamoto Akiko Nishii Atsuki Fujino Akiho Kurahashi Momoka Satoh Yuki Iwasa Koki Okamura Karin Obazaki Ryoto Kumagai Naruya Sakamoto Yuto Tanaka Yukika Kamiya Tetsushi Hoshida Tsuneyasu Kaisho Hiroaki Hemmi Tomoya Katakai Tetsuya Honda Junichi Kikuta Kosuke Kataoka Ryoyo Ikebuchi Taiki Moriya Takahiro Adachi Takeshi Watanabe Masaru Ishii Atsushi Miyawaki Kenji Kabashima Tatyana Chtanova Michio Tomura Sublingual immune cell clusters and dendritic cell distribution in the oral cavity American Society for Clinical Investigation JCI Insight 2379-3708 9 21 null null 20241001 10.1172/jci.insight.167373 read0051714/published_papers/47368849 Qianying Li Kataoka K Yoshimatsu H Miyake T Effects of D-psicose on growth and hyphal development of Candida albicans. EC Microbiology 19 1 1 10 20240100 read0051714/published_papers/46113317 Hideki YOSHIMATSU , Kahori KAWAMURA , Zheqi HUANG , Shijie YANG , Qianying LI , Takaya KITAYAMA , Kayo KOYANAGI , Keita KANO , Takashi DOI , Yoshiaki ONO , Kosuke KATAOKA and Tatsuro MIYAKE 吉松英樹河村佳穂黄哲麒楊世李前穎北山貴也小柳圭代加納慶太土居貴士小野圭昭片岡宏介三宅達郎 Effect of Hinokitiol on Growth and Biofilm Formation of Streptococcus mutans 天然由来成分ヒノキチオールがStreptococcus mutansの増殖およびバイオフィルム形成に与える影響口腔衛生学会雑誌 null null 20240100 10.5834/jdh.74.1_52 read0051714/published_papers/46680352 小柳圭代片岡宏介吉松英樹藤橋浩太郎三宅達郎経鼻ダブルDNAアジュバントにより誘導されたヒト唾液タンパク特異的マウス唾液SIgA抗体はPorphyromonas gingivalisの付着抑制に必要である大阪歯科学会歯科医学 0030-6150 86 1 69 70 20230300 read0051714/published_papers/42468769 片岡宏介吉松英樹栁沢志津子三宅達郎口腔感染症および非感染性疾患予防を目指した粘膜ワクチンの開発 ─新たな経鼻ダブルDNAアジュバントシステムによる健康長寿社会への貢献─ 口腔衛生学会雑誌 null null 20230200 10.5834/jdh.73.1_13 read0051714/published_papers/42468768 Kayo Koyanagi Kosuke Kataoka Hideki Yoshimatsu Kohtaro Fujihashi Tatsuro Miyake Human salivary protein-derived peptides specific-salivary SIgA antibodies enhanced by nasal double DNA adjuvant in mice play an essential role in preventing Porphyromonas gingivalis colonization: an in-vitro study BMC Oral Health null null 20230200 10.1186/s12903-023-02821-6 read0051714/published_papers/39247472 片岡宏介 Development of Mucosal Vaccines as Novel Preventive Methods against Infectious and Noncommunicable Diseases (NCDs): Contribution to a Society of Health and Longevity 口・鼻腔からの感染症および生活習慣病予防をめざす粘膜ワクチン開発-健康長寿社会への貢献を目指して- Journal of Oral Health and Biosciences 四国歯学会雑誌 35 1 27 32 20220900 read0051714/published_papers/39247528 葛西礼衣福井誠栁沢志津子片岡宏介 1,450 ppm フッ化物配合歯磨剤によるブラッシング後の安静時唾液中フッ化物イオンの残存状況に関する報告 JOURNAL OF DENTAL HEALTH 口腔衛生学会雑誌 72 3 173 177 20220730 read0051714/published_papers/39932545 吉松英樹片岡宏介小柳圭代 Li Qiang Ying 小野圭昭河村佳穂里土居貴士三宅達郎ダブルDNAアジュバント経鼻投与による歯周病原菌感染動脈硬化モデルマウスの炎症性サイトカイン発現の影響 (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 72 83 83 20220400 read0051714/published_papers/36365814 Hideki Yoshimatsu Kosuke Kataoka Kohtaro Fujihashi Tatsuro Miyake Yoshiaki Ono A nasal double DNA adjuvant system induces atheroprotective IgM antibodies via dendritic cell-B-1a B cell interactions We previously demonstrated that the dendritic cell (DC)-targeting nasal double DNA adjuvant system, which consists of a DNA plasmid expressing Flt3 ligand (pFL) and CpG oligodeoxynucleotide 1826 (CpG ODN), elicits specific immune responses to various antigens in the mucosal and systemic compartments. Here, we investigated, using phosphorylcholine (PC)-conjugated keyhole limpet hemocyanin (PC-KLH) as an antigen, whether the nasal double DNA adjuvant system induces protective immunity to atherosclerosis in apolipoprotein E-deficient (ApoE KO) mice. Further, we assessed the molecular and cellular mechanisms in the induction of anti-PC-specific immune responses. Nasal immunization with PC-KLH plus pFL and CpG ODN enhanced induction of PC-specific IgM in plasma, peritoneal fluids, and nasal washes when compared with mice administered PC-KLH alone. Of importance, these antibodies exhibited highly specific binding to the PC molecule, and dose-dependent binding to anti-T15 idiotype (AB1-2). Twelve weeks after the last immunization, the nasal double DNA adjuvant system with PC-KLH resulted in a reduction of atherogenesis in the aortic arch of ApoE KO mice. Therefore, we next assessed immunocytological mechanism to induce these antibodies. The nasal double DNA adjuvant system with PC-KLH resulted not only in significantly increased frequencies of CD11c+ DCs in the spleen, peritoneal cavity (PEC), and nasopharyngeal-associated lymphoid tissues (NALT), but also significantly increased expression of a proliferation-inducing ligand and B-cell-activating factor by CD11c+ DCs. In addition, the double DNA adjuvant system induced significantly increased numbers of B-1 B cells in the spleen, PEC, and NALT, and increased expression of transmembrane activator and calcium modulator and cyclophilin ligand interactor on CD5+ B220+ (B-1a) B cells. These findings demonstrated that the nasal double DNA adjuvant system with PC-KLH resulted in the induction of T15-like antibodies in the mucosal and systemic lymphoid tissues through interaction between DCs and B-1a B cells, and inhibited the progression of atherogenesis. Elsevier BV Vaccine 0264-410X 40 8 1116 1127 20220200 10.1016/j.vaccine.2022.01.027 35086743 read0051714/published_papers/39932546 Li Qian Ying 片岡宏介吉松英樹小柳圭代松井正格北山貴也 Huang Zhe Qi Yang SiJie 坂本由紀子河村佳穂里土居貴士小野圭昭三宅達郎希少糖D-プシコースのCandida albicans増殖への影響 (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 72 1 57 57 20220100 read0051714/published_papers/36365819 片岡宏介ダブルDNAアジュバントシステムによる唾液分泌型IgA抗体誘導と抗免疫老化神奈川歯学 56 2 141 143 20211200 read0051714/published_papers/39932548 小柳圭代片岡宏介吉松英樹李前穎河村佳穂里土居貴士三宅達郎ヒト唾液タンパク由来抗原特異的分泌型IgA抗体による歯周病原菌の実験的歯面モデルへの結合抑制効果 (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 71 122 122 20210400 read0051714/published_papers/39932547 吉松英樹片岡宏介小柳圭代李前穎河村佳穂里土居貴士小野圭昭三宅達郎 DNAアジュバントとホスホリルコリン抗原の経鼻投与は脾臓・腹腔内樹状細胞-B1細胞間相互作用を増強する (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 71 124 124 20210400 read0051714/published_papers/39932553 小柳圭代片岡宏介吉松英樹三宅達郎 DNAダブルアジュバンド経鼻投与により唾液に誘導されたヒト唾液タンパク由来ペプチド抗原特異的分泌型IgA抗体は歯周病原菌のスタセリンへの結合を阻害する (NPO)日本歯周病学会日本歯周病学会会誌 1880-408X 62 128 128 20200500 read0051714/published_papers/42965576 上根昌子河村佳穂里加納慶太松井正格小柳圭代土居貴士片岡宏介神光一郎井上直敏太田謙司三宅達郎レセプト・特定健診情報を用いた就労者の医科，歯科の受診割合と医療費に関する疫学研究 (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 70 2 94 102 20200400 read0051714/published_papers/39932554 吉松英樹片岡宏介小柳圭代小渕健二朗河村佳穂里土居貴士神光一郎小野圭昭三宅達郎新規ダブルDNAアジュバントとPC抗原の経鼻投与はT15 idiotypeを誘導し血漿コレステロール値上昇を抑制する (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 70 155 155 20200300 read0051714/published_papers/22939003 Kenjiro Kobuchi Kosuke Kataoka Yoichiro Taguchi Tatsuro Miyake Makoto Umeda Nasal double DNA adjuvant induces salivary FimA-specific secretory IgA antibodies in young and aging mice and blocks Porphyromonas gingivalis binding to a salivary protein Springer Science and Business Media {LLC} BMC Oral Health 19 1 188 196 20191200 10.1186/s12903-019-0886-2 read0051714/published_papers/39932556 吉松英樹片岡宏介小柳圭代三宅達郎小野圭昭 CpG ODNとFlt3 ligand発現DNAプラスミドからなる粘膜アジュバントのホスホリルコリン抗原に対する免疫応答性 (一社)日本障害者歯科学会障害者歯科 2188-9708 40 3 293 293 20190900 read0051714/published_papers/39932540 金山愛加笹部倫世山本眸片岡宏介吉松英樹小柳圭代南部隆之沖永敏則小野圭昭河村佳穂里土居貴士三宅達郎ヒト口腔内細菌及び真菌に対する歯木ニーム抽出液の抗菌効果大阪歯科学会歯科医学 0030-6150 82 2 72 80 20190900 read0051714/published_papers/22939004 金山愛加笹部倫代山本眸片岡宏介吉松英樹小柳圭代南部隆之沖永敏則小野圭昭河村佳穂里土居貴士三宅達郎ヒト口腔内細菌および真菌に対する歯木ニーム抽出液の抗菌効果歯科医学 83 1 null null 20190900 read0051714/published_papers/22939005 加納慶太上根昌子土居貴士福原隆久神光一郎片岡宏介三宅達郎歯科口腔外科における各種感染症の検出に関する検討 82 1 6 11 20190000 read0051714/published_papers/22939018 Jin K 片岡宏介 Wato M Maesoma A Nakatsuka M Kumabe S Uene M Doi T Kataoka K Miyake T Komasa Y Oral complaints and dental clinic visits among Japanese In 1981, the prevalence of dental caries and the mean number of carious teeth per person reached a peak in Japan, and dental health care providers were fully occupied with the treatment of dental caries. More recently, however, the dental health care needs of Japanese people have diversified as a result of increased awareness of oral health, improvements in oral health visits, and the efforts of dental health care providers. In order to determine the current dental healthcare needs of Japanese, it is necessary to understand socioeconomic trends in Japan, such as the acceleration of the aging of society, the low birth rate, and the increased life expectancy. It is also important to understand the living environments of people seeking dental care, the status of people presenting with oral symptoms, and their reasons for dental clinic visits. Dental healthcare providers are often kept fully occupied with their daily practice. It is difficult to say whether they have a full understanding of the socioeconomic influences on dental care or of the actual situations of individuals seeking dental care.This study clarifies the official data on the status of oral complaints, and analyzes the dental clinic attendance behavior of people with oral complaints. The results reveal that while the proportion of people with subjective oral symptoms (toothache, inflamed gingiva or bleeding, and difficulty chewing) has tended to decrease in relative terms over recent years, the peak age group seeking treatment has shifted towards the elderly. The trend toward greater numbers of natural teeth in elderly people is predicted to continue in the future, which implies that demand for oral care among the elderly is likely to further increase. In addition, the proportion of people seeking treatment for acute dental problems has tended to decrease in those over 44 years of age. We also found that among the elderly, a high proportion of visits to medical clinics was for hypertension,eye disease, and lower back pain. When the occupation of the dental patients was considered, although there is a high incidence of oral complaints among unemployed persons, their rate of clinic visits remains low.A pressing issue for the future is to maintain oral health by building an accurate picture of the oral status of people lacking access to regular dental examinations, such as occupational or school health checks, and of the elderly, and eliminating the causes of oral symptoms early. In order to realize this goal, it is essential that dental care practitioners consider the perspectives and individual situations of patients in order to optimally fulfill their roles as family dentists within the community. (J Osaka Dent Univ 2018; 52: 23-29) Osaka Odontological Society 大阪歯科学会 J Osaka Dent Univ. 0475-2058 52 1 23 29 20180000 10.18905/jodu.52.1_23 read0051714/published_papers/22939017 片岡宏介小渕健二朗田口洋一郎上根昌子土居貴士神光一郎梅田誠三宅達郎ヒノキチール経鼻投与による抗原特異的IgA抗体産生への影響歯科医学 81 1 16 23 20180000 read0051714/published_papers/22939016 片岡宏介國友栄治小渕健二朗上根昌子土居貴士三宅達郎ヒノキチオールの唾液分泌型IgA抗体産生に与える影響口腔衛生学会雑誌 68 3 137 144 20180000 read0051714/published_papers/22939006 KATAOKA KosukeJin K Jin K Wato M Toyama T Maesoma A Nakatsuka M Doi T Uene M Kataoka K Miyake T Komasa Y The Dynamics of dental clinics and ensuring a system of dental healthcare provision in Japan. J Osaka Dent Univ. 52 2 157 162 20180000 read0051714/published_papers/22939020 Kosuke Kataoka Yoshiko Fukuyama David E. Briles Tatsuro Miyake Kohtaro Fujihashi Dendritic cell-targeting DNA-based nasal adjuvants for protective mucosal immunity to Streptococcus pneumoniae MICROBIOLOGY AND IMMUNOLOGY 1348-0421 61 6 195 205 20170600 10.1111/1348-0421.12487 read0051714/published_papers/22939021 Jin K 片岡宏介 Doi T Uene M Takayama Y Kataoka K Miyake T Dental and oral health survey of dental hygienist vocational college students: Comparison of students before and after a course on oral hygiene. The socioeconomic situation in Japan is changing drastically, resulting in the need for a variety of dental interventions. Dental healthcare providers therefore need to offer effi cient treatments to prevent dental diseases and take the initiative on providing guidance for oral health. Community dentistry which is the only academic discipline in dentistry to expertly address prevention and health promotion will play a central role in this, and den tal hygienists will play an important role. In the present study, we conducted a questionnairebased survey with intraoral exami nations of firstto thirdyear dental hygiene students enrolled at Osaka Dental University School of Dental Hygienists. The changes in their knowledge and interpretation of dental and oral health after taking a course on oral hygiene were aggregated and analyzed by text mining. After aggregating and analyzing responses to the questionnaire, we found that most students who wanted to become dental hygienists had visited dental clinics 1 to 5 times during their elementary school days, and that most of them answered that they currently had no dental caries. Furthermore, more than 90% of the students reported that they saw themselves working as dental hygienists in 10 years, indicating a strong awareness and attitude that they would continue their profession in the future. In our comparison of stu dents before and after taking a course on oral hygiene, most firstyear students gave an swers closer to the knowledge and interpretation of the general population, while most secondyear students gave interpretations based on professional knowledge and think ing, such as methods of preventing dental diseases learned from their courses. Third year students, however, had a tendency to interpret questions with a focus on dentistry,because their view of prevention and health promotion had been influenced by other clinical courses. Differences in knowledge and interpretation of the field of oral hygiene were dependent on the student's academic year. This suggests that, in order to preserve the concept of oral hygiene required by dental hygienists up into the third academic year, we need to investigate the provision of courses on oral hygiene in a new curriculum that is not bound by the concept of sepa rate curricula in each academic year with traditional fixed semesters. This could be achieved by introducing iterative learning of topics addressed in the first year into the second and third years, or by crossover courses of clinical and basic subjects. (J Osaka Dent Univ 2017 ;51 : 916) Osaka Odontological Society 大阪歯科学会 J Osaka Dent Univ. 51 1 9 16 20170000 10.18905/jodu.51.1_9 read0051714/published_papers/22939019 Jin K 片岡宏介 Nakatsuka M Maesoma A Wato M Uene M Doi T Kataoka K Miyake T Komasa Y Employment status of dental hygienists in Japan Japan has become a superaged society at a precipitous rate in recent years, and there is a need for comprehensive care targeting the everincreasing number of elderly and others requiring care. There is also a growing awareness in medical settings that mainte nance of satisfactory intraoral conditions and function is linked to whole body health. Consequently, there is a need for implementation of oral care that will allow the elderly and persons requiring care to enjoy their meals. In addition, a system is needed that en compasses both the fields of medicine and dentistry for treatment of perioperative and terminal stage patients. In both situations, the dental hygienist plays a central role. This study used the results of national statistical surveys carried out by the Japanese Ministry of Health, Labour and Welfare to clarify the employment status of dental hygien ists. We also evaluated the status of treatment and guidance performed by dental hygien ists in medical settings and investigated the future supply and demand for dental hygien ists. The results showed that almost half the individuals who pass the national examination to qualify as dental hygienists do not find employment in this field. In subgroup analysis stratified by age, the number of new graduates who leave their job in dental hygiene within three to four years of starting, or who do not start work, is increasing. The rea sons for this low employment rate must be determined. At the same time, the number of dental hygienists over 30 years of age who are employed is increasing, and this is par ticularly true for those over 50 years of age. The supply and demand for dental hygien ists should be studied. An understanding of the reemployment of qualified dental hygien ists should include how improvements in their worklife balance might allow them to make use of their qualifications throughout their working lives. Approximately 90% of dental hygienists currently work at dental clinics ;however,growth in the number of these clinics has recently shown signs of slowing. In addition,the number of dental hygienists per clinic is increasing very slowly. Nonetheless, the de mand for dental hygienists at the frontline of medicine and medical care is increasing due to the growing recognition of the need for specialized intraoral management during the perioperative period in hospitals and other health care settings. The demand for den tal hygienists will probably continue to increase in the future. (J Osaka Dent Univ 2017; 51: 99-104) Osaka Odontological Society 大阪歯科学会 J Osaka Dent Univ. 0475-2058 51 2 99 104 20170000 10.18905/jodu.51.2_99 read0051714/published_papers/22939022 片岡宏介土居貴士神光一郎上根昌子伊藤博夫落合(栗田)智子三宅達郎樹状細胞を活性化する経鼻アジュバントの開発-感染症・NCD制御を目指す粘膜ワクチン- 口腔衛生学会雑誌 67 3 2 10 20160000 read0051714/published_papers/47272060 高橋悦久片岡宏介 Indalao Irene Lorinda 堺聡子木戸博インフルエンザ感染時においてタミフルとクラリスロマイシンの併用で気道粘膜での抗インフルエンザ特異IgA抗体の増強が認められた (公社)日本生化学会日本生化学会大会プログラム・講演要旨集 86 2P 460 20130900 read0051714/published_papers/22939024 Yoshiko Fukuyama Daisuke Tokuhara Shinichi Sekine Kazuyoshi Aso Kosuke Kataoka Julia Davydova Masato Yamamoto Rebekah S. Gilbert Yuka Tokuhara Keiko Fujihashi Jun Kunisawa Yoshikazu Yuki Hiroshi Kiyono Jerry R. McGhee Kohtaro Fujihashi Potential Roles of CCR5+ CCR6+ Dendritic Cells Induced by Nasal Ovalbumin plus Flt3 Ligand Expressing Adenovirus for Mucosal IgA Responses PLoS ONE 1932-6203 8 4 60453 null 20130402 10.1371/journal.pone.0060453 23565250 2-s2.0-84875685651 read0051714/published_papers/46810722 天羽崇日野出大輔平島美穂吉岡昌美横山正明玉谷香奈子福井誠伊藤博夫片岡宏介舌苔中の歯周病関連細菌と口臭との関連性 (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 63 3 289 289 20130400 read0051714/published_papers/34713464 武川香織真杉幸江高橋侑子十川悠香坂本治美片岡宏介玉谷香奈子吉岡昌美日野出大輔妊婦歯科健康診査受診者の妊娠中の歯周状態と出産の状況について(第2報) (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 63 3 289 290 20130400 read0051714/published_papers/37576755 木戸博高橋悦久片岡宏介水野大木本貴士藤本知佐 Indalao Irene L. インフルエンザ感染症における粘膜免疫とその制御による治療効果の増強 (株)医薬情報研究所新薬と臨牀 0559-8672 61 12 2459 2470 20121200 read0051714/published_papers/22939026 Etsuhisa Takahashi Kosuke Kataoka Irene L. Indalao Keiko Konoha Kazuyuki Fujii Junji Chida Dai Mizuno Kohtaro Fujihashi Hiroshi Kido Oral Clarithromycin Enhances Airway Immunoglobulin A (IgA) Immunity through Induction of IgA Class Switching Recombination and B-Cell-Activating Factor of the Tumor Necrosis Factor Family Molecule on Mucosal Dendritic Cells in Mice Infected with Influenza A Virus JOURNAL OF VIROLOGY 0022-538X 86 20 10924 10934 20121000 10.1128/JVI.01207-12 read0051714/published_papers/22939027 Masaki Goto Agnieszka Wilk Kosuke Kataoka Shirish Chodankar Nobutake Tamai Makoto Fukui Joachim Kohlbrecher Hiro-O Ito Hitoshi Matsuki Study on the Subgel-Phase Formation Using an Asymmetric Phospholipid Bilayer Membrane by High-Pressure Fluorometry LANGMUIR 0743-7463 28 33 12191 12198 20120800 10.1021/la3020173 read0051714/published_papers/46809211 天羽崇平島美穂吉岡昌美福井誠片岡宏介日野出大輔舌苔沈着，唾液中の歯周病原細菌と口臭との関連性 (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 62 2 251 251 20120400 read0051714/published_papers/41268838 渋谷敦人小林弘茂福井隆夫岡田寿朗豊嶋健治福井誠片岡宏介伊藤博夫奥弘文平成22年度に香川県内で実施された成人歯科健診モデル事業実施報告(香川県歯科医師会) (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 62 3 337 337 20120400 read0051714/published_papers/34450752 武川香織真杉幸江十川悠香坂本治美福井誠片岡宏介吉岡昌美日野出大輔妊婦歯科健康診査受診者の妊娠中の歯周状態と出産の状況について (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 62 3 339 339 20120400 read0051714/published_papers/22939028 Yoshiko Fujkuyama Daisuke Tokuhara Kosuke Kataoka Rebekah S. Gilbert Jerry R. McGhee Yoshikazu Yuki Hiroshi Kiyono Kohtaro Fujihashi Novel vaccine development strategies for inducing mucosal immunity EXPERT REVIEW OF VACCINES 1476-0584 11 3 367 379 20120300 10.1586/ERV.11.196 read0051714/published_papers/22939029 Yoshiko Fukuyama Daisuke Tokuhara Shinichi Sekine Kosuke Kataoka Jonathan D. Markham Allyson R. Irwin Grace H. Moon Yuka Tokuhara Keiko Fujihashi Julia Davydova Masato Yamamoto Rebekah S. Gilbert Kohtaro Fujihashi Notch-ligand expression by NALT dendritic cells regulates mucosal Th1-and Th2-type responses BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 0006-291X 418 1 6 11 20120200 10.1016/j.bbrc.2011.12.046 read0051714/published_papers/22939031 Kosuke Kataoka Keiko Fujihashi Yutaka Terao Rebekah S. Gilbert Shinichi Sekine Ryoki Kobayashi Yoshiko Fukuyama Shigetada Kawabata Kohtaro Fujihashi Oral-Nasopharyngeal Dendritic Cells Mediate T Cell-Independent IgA Class Switching on B-1 B Cells PLOS ONE 1932-6203 6 9 25396 null 20110900 10.1371/journal.pone.0025396 read0051714/published_papers/22939032 Tselmeg Baatarjav Kosuke Kataoka Rebekah S. Gilbert Yutaka Terao Makoto Fukui Masaki Goto Shigetada Kawabata Masafumi Yamamoto Kohtaro Fujihashi Hiro-O Ito Mucosal immune features to phosphorylcholine by nasal Flt3 ligand cDNA-based vaccination VACCINE 0264-410X 29 34 5747 5757 20110800 10.1016/j.vaccine.2011.05.097 read0051714/published_papers/22939037 Kosuke Kataoka Kohtaro Fujihashi Keita Oma Yoshiko Fukuyama Susan K. Hollingshead Shinichi Sekine Shigetada Kawabata Hiro-O Ito David E. Briles Kazunori Oishi The Nasal Dendritic Cell-Targeting Flt3 Ligand as a Safe Adjuvant Elicits Effective Protection against Fatal Pneumococcal Pneumonia INFECTION AND IMMUNITY 0019-9567 79 7 2819 2828 20110700 10.1128/IAI.01360-10 read0051714/published_papers/22939040 R. Kobayashi T. Kono B. A. Bolerjack Y. Fukuyama R. S. Gilbert K. Fujihashi J. Ruby K. Kataoka M. Wada M. Yamamoto K. Fujihashi Induction of IL-10-producing CD4(+) T-cells in Chronic Periodontitis JOURNAL OF DENTAL RESEARCH 1544-0591 90 5 653 658 20110500 10.1177/0022034510397838 read0051714/published_papers/22939035 Hiroshi Kido Etsuhisa Takahashi Kosuke Kataoka Kazuyuki Fujii Satoshi Suzuki Kazuhiro Iwase Chika Ito Attenuation of respiratory immune responses by antiviral neuraminidase inhibitor treatment and boost of mucosal immunoglobulin A response by co-administration of immuno-modulator clarithromycin in paediatric influenza INFLUENZA AND OTHER RESPIRATORY VIRUSES 1750-2640 5 240 243 20110500 read0051714/published_papers/22939042 Yoshiko Fukuyama Janice D. King Kosuke Kataoka Ryoki Kobayashi Rebekah S. Gilbert Susan K. Hollingshead David E. Briles Kohtaro Fujihashi A Combination of Flt3 Ligand cDNA and CpG Oligodeoxynucleotide as Nasal Adjuvant Elicits Protective Secretory-IgA Immunity to Streptococcus pneumoniae in Aged Mice JOURNAL OF IMMUNOLOGY 0022-1767 186 4 2454 2461 20110200 10.4049/jimmunol.1002837 read0051714/published_papers/22939046 Makoto Fukui Daisuke Hinode Masaaki Yokoyama Masami Yoshioka Kosuke Kataoka Hiro-O Ito Levels of salivary stress markers in patients with anxiety about halitosis ARCHIVES OF ORAL BIOLOGY 0003-9969 55 11 842 847 20101100 10.1016/j.archoralbio.2010.07.014 read0051714/published_papers/41268840 三崎昌和油谷一裕渋谷敦人岡田寿朗阿部直樹豊嶋健治福井誠片岡宏介伊藤博夫奥弘文平成21年度に香川県内で実施された成人歯科健診モデル事業実施報告 (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 60 5 608 608 20101000 read0051714/published_papers/22939048 Etsuhisa Takahashi Kosuke Kataoka Kazuyuki Fujii Junji Chida Dai Mizuno Makoto Fukui Hiro-O Ito Kohtaro Fujihashi Hiroshi Kido Attenuation of inducible respiratory immune responses by oseltamivir treatment in mice infected with influenza A virus MICROBES AND INFECTION 1286-4579 12 10 778 783 20100900 10.1016/j.micinf.2010.04.013 read0051714/published_papers/22939047 Yoshiko Fukuyama Janice D. King Kosuke Kataoka Ryoki Kobayashi Rebekah S. Gilbert Kazunori Oishi Susan K. Hollingshead David E. Briles Kohtaro Fujihashi Secretory-IgA Antibodies Play an Important Role in the Immunity to Streptococcus pneumoniae JOURNAL OF IMMUNOLOGY 0022-1767 185 3 1755 1762 20100800 10.4049/jimmunol.1000831 read0051714/published_papers/26991366 Hashizume, T Hashizume Tomomi tanaka, S Tanaka Shunichi Kaminogawa, S Kaminogawa Shuichi Hosono, A Hosono Akira Kataoka, K Kataoka Kosuke Shinozaki-Kuwahara, N Shinozaki-Kuwahara Noriko Kweon, M. N Kweon Mi-Na Kurita-Ochiai, T Kurita-Ochiai Tomoko Yamamoto, M Yamamoto Masafumi Mucosal Immunity in CD4-Deficient mice: Direct evidence for mucosal IgA antibody responses. Mucosal Immunity in CD4-Deficient Mice: Direct Evidence for Mucosal IgA Antibody Responses CD4+ T cells and their derived cytokines are major contributors to the induction and regulation of antigen-specific antibody responses in both mucosal and systemic compartments. Past studies have shown that antigen-specific mucosal immune responses are impaired in CD4 gene-disrupted (CD4-/-) mice ; however, surface IgA+ B cells are found to co-localize to the germinal center area and CD4-CD8- (double negative) T cells replace CD4+ T cells in the Peyer's patches. Thus, we investigated whether different oral delivery systems could elicit mucosal immunity. Here we show that CD4-/- mice are capable of eliciting secretory IgA antibody responses. Oral immunization using tetanus toxoid (TT) with cholera toxin as a mucosal adjuvant resulted in mucosal IgA and serum IgG anti-TT antibody responses. Furthermore, antigen-specific T cell proliferative responses showed that significant TT-specific proliferative responses were induced in the CD4-CD8-, but not CD4-CD8+, T cells from Peyer's patches of CD4-/- mice given TT plus cholera toxin orally. These results suggest that antigen-specific mucosal IgA antibody responses may be induced by CD4-CD8- T cells in the absence of CD4+ T cells. Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo Int. J. Oral-Med. Sci. International Journal of Oral-Medical Sciences 1347-9733 8 3 151-156 156 20100300 10.5466/ijoms.8.151 read0051714/published_papers/22939049 Hidetaka Nakagaki Shinichi Sekine Yutaka Terao Masahiro Toe Muneo Tanaka Hiro-O Ito Shigetada Kawabata Satoshi Shizukuishi Kohtaro Fujihashi Kosuke Kataoka Fusobacterium nucleatum Envelope Protein FomA Is Immunogenic and Binds to the Salivary Statherin-Derived Peptide INFECTION AND IMMUNITY 0019-9567 78 3 1185 1192 20100300 10.1128/IAI.01224-09 read0051714/published_papers/22939055 Keita Oma Jizi Zhao Hirokazu Ezoe Yukihiro Akeda Shohei Koyama Ken J. Ishii Kosuke Kataoka Kazunori Oishi Intranasal immunization with a mixture of PspA and a Toll-like receptor agonist induces specific antibodies and enhances bacterial clearance in the airways of mice VACCINE 1873-2518 27 24 3181 3188 20090500 10.1016/j.vaccine.2009.03.055 read0051714/published_papers/22939053 Yokoyama M 片岡宏介 Fukui M Masuda K Takamatsu N Okada J Takebe H Kataoka K Ito H.-O Measurement of the total number of bacteria in saliva using quantitative real-time PCR (qPCR): Intention to evaluate oral hygiene status. The state of oral hygiene has been evaluated by employing various indices, such as the Oral Hygiene Index, Plaque Index, Patient Hygiene Performance, and Plaque Control Record (O'Leary's PCR). Measuring the scores of these indices is time- and labor-consuming, requiring manual reading and recording by professionals, standardization of examination methods. In contrast, measuring the total number of bacteria in saliva using quantitative real-time PCR (qPCR) allows the straightforward collection and storage of samples, making it possible to measure many samples at the same time. This technique facilitates the quantification of the number of bacteria within a shorter time than the culture method. In the present study, the total number of bacteria measured by qPCR was found to be significantly decreased (p<0.05) in stimulated whole saliva collected from 5 volunteers by chewing paraffin after tooth cleaning. These findings were in agreement with the decreases shown by Greene's Debris Index and O'Leary's PCR values. There was a significant correlation (r=0.55, p<0.01) between the salivary bacterial counts measured by qPCR from 81 subjects before and after a 4-month interval. These findings suggest that it is possible to measure the total number of bacteria in saliva using qPCR. This technique is convenient and is suggested to be applicable for assessing the oral hygiene status in the future. 口腔清潔度の指標としては,歯面への歯垢の付着度を測定する方法が一般的であるが,診査や記録等の人材的,時間的負担を要することから,集団歯科健診で用いられることは少ない.集団歯科健診においてう蝕や歯周疾患,口臭症のリスクを評価するために,簡便かつ有効な口腔清潔度の評価法の開発が望まれている.リアルタイム定量PCR(qPCR)法による唾液中の総細菌数の測定は,検体の採取や保管が容易で多検体の一括処理が可能であることから,本目的に合致する有望な測定法であると考えられる.本研究では,qPCR法を用いた唾液中の総細菌数の測定が口腔清潔度の評価法として使用できるかどうか検討を行った.5名のボランティアから,ブラッシング中断時と再開後の2回,刺激唾液を採取し,唾液中の総細菌数をqPCR法により測定したところ,Debris Index,O'LearyのPCR値との間に相関は示されなかったものの,ブラッシング再開後では,中断時に比べ,有意に減少した.81名の被験者から4ヵ月の間隔を空けて2回刺激唾液を採取し,唾液中の総細菌数をqPCR法により測定したところ,前後の間に有意な正の相関を認めた.以上の結果より,qPCR法によって唾液中の総細菌数を測定することが可能であることが示された.本法は非常に簡便であることから,今後,口腔清潔度を検討する分野において応用できる可能性が考えられる. The Japanese Society for Dental Health 有限責任中間法人日本口腔衛生学会 Journal of dental health 口腔衛生学会雑誌 0023-2831 59 3 183 189 20090000 read0051714/published_papers/22939057 Nobuko Nishida Yumiko Yamamoto Muneo Tanaka Kosuke Kataoka Masae Kuboniwa Kunio Nakayarna Kanehisa Morimoto Satoshi Shizukuishi Association Between Involuntary Smoking and Salivary Markers Related to Periodontitis: A 2-Year Longitudinal Study JOURNAL OF PERIODONTOLOGY 1943-3670 79 12 2233 2240 20081200 10.1902/jop.2008.080149 read0051714/published_papers/22939056 Tatsuya Fukuiwa Shinichi Sekine Ryoki Kobayashi Hideaki Suzuki Kosuke Kataoka Rebekah S. Gilbert Yuichi Kurono Prosper N. Boyaka Arthur M. Krieg Jerry R. McGhee Kohtaro Fujihashi A combination of Flt3 ligand cDNA and CpG ODN as nasal adjuvant elicits NALT dendritic cells for prolonged mucosal immunity VACCINE 0264-410X 26 37 4849 4859 20080900 10.1016/j.vaccine.2008.06.091 read0051714/published_papers/22939060 Hideki Nagata Yoshika Inagaki Muneo Tanaka Miki Ojima Kosuke Kataoka Masae Kuboniwa Nobuko Nishida Katsurnasa Shimizu Kenji Osawa Satoshi Shizukuishi Effect of eucalyptus extract chewing gum on periodontal health: A double-masked, randomized trial JOURNAL OF PERIODONTOLOGY 1943-3670 79 8 1378 1385 20080800 10.1902/jop.2008.070622 read0051714/published_papers/22939059 Shinichi Sekine Kosuke Kataoka Yoshiko Fukuyama Yasuo Adachi Julia Davydova Masato Yamamoto Ryoki Kohayashi Keiko Fujihashi Hideaki Suzuki David T. Curiel Satoshi Shizukuishi Jerry R. McGhee Kohtaro Fujihashi A novel adenovirus expressing Flt3 ligand enhances mucosal immunity by inducing mature nasopharyngeal-associated lymphoreticular tissue dendritic cell migration JOURNAL OF IMMUNOLOGY 0022-1767 180 12 8126 8134 20080600 read0051714/published_papers/22939062 KATAOKA Kosuke 雫石聰田中宗雄小島美樹西田伸子永田英樹片岡宏介久保庭雅恵「歯周病と喫煙との関連性についてのEBM」 Cutting-Edge Biodentistry 生命歯科医学のカッティング・エッジ 109 119 20080000 read0051714/published_papers/22939066 Tomomi Hashizume Fumiki Momoi Tomoko Kurita-Ochiai Shuichi Kaminogawa Akira Hosono Kosuke Kataoka Noriko Shinozaki-Kuwahara Mi-Na Kweon Masafumi Yamamoto Isolated lymphoid follicles are not IgA inductive sites for recombinant Salmonella BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 0006-291X 360 2 388 393 20070800 10.1016/j.bbrc.2007.06.096 read0051714/published_papers/22939071 Kosuke Kataoka 片岡宏介 Keiko Fujihashi Shinichi Sekine Tatsuya Fukuiwa Ryoki Kobayashi Hideaki Suzuki Hideki Nagata Kiyoshi Takatsu Satoshi Shizukuishi Jerry R. McGhee Kohtaro Fujihashi Nasal cholera toxin elicits IL-5 and IL-5 receptor alpha-chain expressing B-1a B cells for innate mucosal IgA antibody responses Nasal cholera toxin elicits IL-5 and IL-5 receptor chain expressing B-1a B cells for innate mucosal IgA antibody responses. JOURNAL OF IMMUNOLOGY J. Immunol. 0022-1767 178 10 6058 6065 20070500 read0051714/published_papers/22939074 Yukari Hagiwara Yuki I. Kawamura Kosuke Kataoka Bibi Rahima Raymond J. Jackson Katsuhiro Komase Taeko Dohi Prosper N. Boyaka Yoshifumi Takeda Hiroshi Kiyono Jerry R. McGhee Kohtaro Fujihashi A second generation of double mutant cholera toxin adjuvants: Enhanced immunity without intracellular trafficking JOURNAL OF IMMUNOLOGY 0022-1767 177 5 3045 3054 20060900 read0051714/published_papers/22939111 H Nagata Y Inagaki Y Yamamoto K Maeda K Kataoka K Osawa S Shizukuishi Inhibitory effects of macrocarpals on the biological activity of Porphyromonas gingivalis and other periodontopathic bacteria ORAL MICROBIOLOGY AND IMMUNOLOGY 0902-0055 21 3 159 163 20060600 read0051714/published_papers/22939076 雫石聰片岡宏介全身疾患と歯周病口の中は''危険がいっぱい''リスクファクターとしての喫煙日本歯科評論別冊The Nippon Dental Review 137 144 20060000 read0051714/published_papers/22939113 Y Terao S Okamoto K Kataoka S Hamada S Kawabata Protective immunity against Streptococcus pyogenes challenge in mice after immunization with fibronectin-binding protein JOURNAL OF INFECTIOUS DISEASES 1537-6613 192 12 2081 2091 20051200 read0051714/published_papers/22939116 Kataoka K Kataoka Kosuke Suzuki H Suzuki Hideaki Sims R. L Sims Rebekah L. Sekine S Sekine Shinichi Kobayashi R Kobayashi Ryoki Fujihashi K Fujihashi Keiko Fukuiwa T. B Fukuiwa Tatsuya Mizuno T Mizuno Takanari Jackson R. J Jackson Raymond J. Boyaka P. N Boyaka Prosper N. McGhee J. R McGhee Jerry R. Fujihashi K Fujihashi Kohtaro Dendritic cells targeting Flt3 ligands as molecular mucosal adjuvant. Nasal antigen plus mucosal adjuvant delivery has emerged as perhaps the most effective route for induction of both mucosal and peripheral immune response. It has been shown that dendritic cells (DCs) played essential roles for the induction of both immunity and tolerance. Thus, nasal application of CpG ODN targeting plasmacytoid DCs effectively induced antigen-specific immunity. Our recent studies showed that nasal or oral immunization with protein antigen, ovalbumin plus plasmid encoding Flt3 ligand (pFL) cDNA as adjuvant effectively elicited OVA-specific immune responses in both mucosal and systemic lymphoid tissues. The numbers of (DCs) in mice given pFL as mucosal adjuvant were significantly increased. Further, these expanded DCs were activated and expressed MHC II and co-stimulatory molecules (CD40, CD80 and CD86). These results show that mucosal delivery of pFL is DCs targeting adjuvant system that effectively induce both mucosal and systemic antigen-specific immune responses. Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo Int. J. Oral-Med. Sci. 1347-9733 3 3 111 120 20050000 10.5466/ijoms.3.111 read0051714/published_papers/22939130 M Tanaka H Anguri A Nonaka K Kataoka H Nagata J Kita S Shizukuishi Clinical assessment of oral malodor by the electronic nose system JOURNAL OF DENTAL RESEARCH 0022-0345 83 4 317 321 20040400 read0051714/published_papers/22939124 Maeda M Nagata H Kuboniwa M Kataoka K Nishida N Tanaka M Shizukuishi S Characterization of binding of Streptococcus oralis glyceraldehydes-3-phosphate dehydroenase to Porphyromonas gingivalis major fimbriae. Binding of Streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to Porphyromonas gingivalis fimbriae was characterized via a biomolecular interaction analysis system. The interaction was specific, and the association constant value was 4.34 x 10(7) M(-1), suggesting that S. oralis GAPDH functions as a dominant receptor for P. gingivalis and contributes to P. gingivalis colonization. Infect. Immun. 0019-9567 72 9 5475 5479 20040000 15322048 read0051714/published_papers/22939121 Tanaka M Yamamoto Y Kuboniwa M Nonaka A Nishida N Maeda K Kataoka K Nagata H Shizukuishi S Contribution of periodontal pathogens on tongue dorsa analyzed with real-time PCR to oral malodor. Oral malodor is considered to originate primarily from tongue microbiota populations. However, the relationship between oral malodor and tongue microbiota remains unclear. In this study, tongue periodontal pathogens were analyzed via real-time PCR, and the association between oral malodor and tongue periodontal pathogens, including Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, Prevotella nigrescens and Treponema denticola, was examined. The subject population consisted of 29 individuals with and 10 healthy persons without oral malodor. Oral malodor was assessed by organoleptic test and volatile sulfur compound (VSC) levels as measured by gas chromatography. Real-time PCR was conducted for anaerobes in tongue biofilm samples employing a LightCycler system; furthermore, bacterial proportion served as a quantitative parameter. Among the five anaerobes, only T. forsythia displayed higher proportions in malodor subjects than corresponding values in healthy controls. Proportions of P. intermedia and P. nigrescens correlated strongly with hydrogen sulfide concentration. Proportions of P. gingivalis and P. nigrescens also exhibited strong correlation with methyl mercaptan concentration. The correlation coefficient between the proportion of the total of the five anaerobes and total VSC level (r = 0.88) was greater than that between bacterial proportion and organoleptic score (r = 0.29). When a linear regression analysis was performed utilizing the proportion of each of the five periodontal pathogens as an independent variable, the explanatory power of these independent variables revealed 81% for total VSC level and 16% for organoleptic score. These results suggest that these five periodontal pathogens on tongue dorsa may contribute greatly to VSC production. Microbes. Infect. 1286-4579 6 12 1078 1083 20040000 15380777 read0051714/published_papers/22939118 Maeda K Nagata H Nonaka A Kataoka K Tanaka M Shizukuishi S Oral streptococcal glyceraldehydes-3-phosphate dehydrogenase mediates interaction with Porphyromonas gingivalis fimbriae. Microbes Infect. 6 13 1163 1170 20040000 read0051714/published_papers/22939136 Y Hagiwara McGhee, JR K Fujihashi R Kobayashi N Yoshino K Kataoka Y Etani MN Kweon S Tamura T Kurata Y Takeda H Kiyono K Fujihashi Protective mucosal immunity in aging is associated with functional CD4(+) T cells in nasopharyngeal-associated lymphoreticular tissue JOURNAL OF IMMUNOLOGY 0022-1767 170 4 1754 1762 20030200 read0051714/published_papers/22939134 広田孝子越山香里今井奈保子青江智子片岡宏介大学アメリカンフットボール選手における大豆・大豆製品摂取の骨密度，体組成および疲労度に及ぼす影響不二たん白質研究振興財団大豆たん白質研究 1344-4050 6 24 140 146 20030000 read0051714/published_papers/22939150 越山香里広田孝子今井奈保子片岡宏介抗酸化ビタミン等，栄養豊富な食品摂取による疲労回復への影響疲労研究会疲労と休養の科学 0913-0241 17 1 99 107 20020000 read0051714/published_papers/22939152 A Amano Nakagawa, I K Kataoka Morisaki, I S Hamada Distribution of Porphyromonas gingivalis strains with fimA genotypes in periodontitis patients JOURNAL OF CLINICAL MICROBIOLOGY 0095-1137 37 5 1426 1430 19990500 read0051714/published_papers/22939155 天野敦雄久保庭雅恵堀江博片岡宏介永田英樹雫石聰温度ストレスがporphyromonas gingivalisの付着能および抗原性に及ぼす影響. Porphyromonas gingivalis ATCC 33277株を39℃で培養し2℃の温度ストレスを加えると,37℃培養に比べ菌体表層の線毛構造物の発現が著しく抑制されると共に,ヒト全唾液被覆ハイドロキシアパタイトビーズ(HAP)へのP.gingivalisの付着能が著しく減少した.高プロリンタンパク質及びスタセリンで被覆したHAPと菌体との結合も顕著な減少を示した.更に,本菌のStreptococcus oralisとの共凝集能も92%の減少を示した.全菌体抽出物のウエスタンブロットと歯周炎患者血清との反応バンドで37℃と39℃培養菌の間に差が認められ,39℃培養菌において既存バンドの濃度の減少と新しいバンドの検出が観察され,両菌体の血清との反応性には相違が認められた (NPO)日本歯周病学会日本歯周病学会雑誌 1880-408X 39 1 86 92 19970000 read0051714/published_papers/22939156 A Amano K Kataoka PA Raj RJ Genco S Shizukuishi Binding sites of salivary statherin for Porphyromonas gingivalis recombinant fimbrillin INFECTION AND IMMUNITY 0019-9567 64 10 4249 4254 19961000 read0051714/published_papers/22939158 HANIOKA Takashi 埴岡隆 KATAOKA Kosuke 片岡宏介 TANAKA Muneo 田中宗雄 SHIZUKUISHI Satoshi 雫石聰 Smoking Habits and Attitudes towards Smoking of Dental Students at Three Schools in West Japan 西日本3大学の歯学生の喫煙行動および喫煙と健康に関する意識調査. To encourage smoking-cessation counseling by oral health team, we surveyed the smoking habits and attitudes of 213 students at three dental schools in West Japan with a self-administered questionnaire. The percentage of smoking was 34%. About 80% of the smokers started smoking in their university days. Many smokers (70%) had already made serious attempts to quit but their reasons for not smoking were personal and there were few social reasons. Most considered smoking harmful to health with lower rates in smokers, but there was important ignorance about tobacco as a major cause of specific diseases. Many students (70%) were concerned with advising patients whom they knew to have smoking-related disease, but few accepted a preventive role in advising patients. Most lacked confidence in their counseling skills and there was little knowledge of public health measures for smoking control, although many students (70%) considered that health worker should receive smoking-cessation counseling training. These attitudes towards smoking were similar in the three schools. These results provide important challenge to dental education regarding smoking and health consequences, and regarding counseling strategies of smoking cessation. 口腔保健従事者による禁煙指導の推進を計るため,喫煙行動および喫煙と健康に関する意識に関して,西日本の3大学で歯学生213名を対象として質問票による調査を行った．調査対象の喫煙率は全体で34%であり,その8割が18歳以後に喫煙を開始したと回答した．喫煙者の70%に禁煙試行の経験があったが,喫煙しない理由は個人的理由に基づくものが多く,社会的理由によるものは少なかった．喫煙は健康に確実に有害であるとの回答は約70%であったが,現喫煙者では約半数であった．喫煙が関連疾患の主原因であると回答した者は多くの疾患で少なかった．将来,禁煙指導や助言を必ずすると回答した者は,「患者が症状を訴えているなどの場合」には70%であったが,「症状も関連疾患もなく,質問もされない場合」には3%と予防の意識は低かった．歯科医師による禁煙指導に関して「喫煙に関する知識は十分である」と回答した者はほとんどなく,また,公衆喫煙対策に関する意見に強く賛成する者も少なかったが,7割は「医療従事者には禁煙希望者へのカウンセリングのための訓練が必要だ」と回答した．これらの態度は各大学間で類似していた．以上のことから,歯学生に対する防煙,禁煙指導教育の必要性が示唆された． Japanese Society for Oral Health 一般社団法人口腔衛生学会 JOURNAL OF DENTAL HEALTH 日本口腔衛生学会雑誌 0023-2831 46 1 63 71 19960000 10.5834/jdh.46.1_63 read0051714/published_papers/22939159 A AMANO M KUBONIWA K KATAOKA K TAZAKI E INOSHITA H NAGATA H TAMAGAWA S SHIZUKUISHI BINDING OF HEMOGLOBIN BY PORPHYROMONAS-GINGIVALIS FEMS MICROBIOLOGY LETTERS 0378-1097 134 1 63 67 19951200 read0051714/published_papers/22939162 S SHIZUKUISHI K TAZAKI E INOSHITA K KATAOKA T HANIOKA A AMANO EFFECT OF CONCENTRATION OF COMPOUNDS CONTAINING IRON ON THE GROWTH OF PORPHYROMONAS-GINGIVALIS FEMS MICROBIOLOGY LETTERS 0378-1097 131 3 313 317 19950900 read0051714/published_papers/22939165 Hanioka T Tanaka M Kataoka K Ojima M Shizukuishi S Clinical evaluation of the plaque removal efficacy of three toothbrushes. J. Clin. Dent. 6 1 113 116 19950000 read0051714/published_papers/22939174 H NAGATA A AMANO M OJIMA M TANAKA K KATAOKA S SHIZUKUISHI EFFECT OF BINDING OF FIBRINOGEN TO EACH BACTERIUM ON COAGGREGATION BETWEEN PORPHYROMONAS-GINGIVALIS AND STREPTOCOCCUS-ORALIS ORAL MICROBIOLOGY AND IMMUNOLOGY 0902-0055 9 6 359 363 19941200 read0051714/published_papers/22939172 HANIOKA Takashi 埴岡隆 TANAKA Muneo 田中宗雄 OJIMA Miki 小島美樹 KATAOKA Kosuke 片岡宏介 NAGATA Hideki 永田英樹 SHIZUKUISHI Satoshi 雫石聰 Epidemiologic Study of Gingival Recession and Cervical Abrasion. As Related to Age, Toothbrushing and Smoking.:As Related to Age, Toothbrushing and Smoking 歯肉退縮と歯頚部摩耗についての疫学的研究-年齢，ブラッシング習慣および喫煙習慣との関連について- The aim of this study was to examine the association of gingival recession and cervical abrasion with age, toothbrushing, and cigarette smoking. One hundred and fifty-seven male employees of a transportation company in Osaka Prefecture (22-63yr of age, average 40.1 years) participated in this study. The prevalence of gingival recession and cervical abrasion was 76% and 60%, respectively. The mean number of surfaces with recession and abrasion were 6.8 and 3.0, respectively. The prevalence rate and the mean number of surfaces in each disease increased with age. The prevalence rate in subjects using the horizontal brushing technique was high in both diseases. Brushing technique was significantly associated with prevalence of both diseases (p<0.05). The mean number of surfaces with recession in subjects who brush with the horizontal technique was significantly higher than those who use varying methods (p<0.05). According to analyses using Hayashi's quantification methods, age showed the strongest association with prevalence rate and number of surfaces in both diseases among the items employed in this study. On each occasion, brushing frequency or brushing technique showed stronger association than amount of dentifrice and bristle stiffness. Cigarette smoking also showed a relatively strong correlation with gingival recession. However, the association between cigarette smoking and cervical abrasion was very weak. These results indicate that gingival recession and cervical abrasion are associated with age and brushing habits such as brushing frequency and technique, and suggest that gingival recession is independently linked to cigarette smoking. Japanese Society for Oral Health 一般社団法人口腔衛生学会 JOURNAL OF DENTAL HEALTH 日本口腔衛生学会雑誌 0023-2831 44 2 202 210 19940000 10.5834/jdh.44.202 read0051714/published_papers/22939171 埴岡隆片岡宏介田中宗雄雫石聰ナトリウムラウリルジアミノエチルグリシンを含む洗口剤の歯垢形成に及ぼす短期的影響大阪大学歯学会雑誌 39 2 315 318 19940000 read0051714/misc/42468766 片岡宏介國友栄治吉松英樹土居貴士上根昌子三宅達郎ヒノキチオール経鼻投与後における老齢マウス唾液IgA抗体分泌の影響 (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 69 170 170 20190400 read0051714/misc/37254933 木戸博高橋悦久片岡宏介インフルエンザとマクロライド気道の生体防御能,粘膜免疫を増強するマクロライド系薬とインフルエンザ感染 (小特集マクロライド系薬の新作用とその有用性 : 臨床としての新たな展開) 医薬ジャーナル社医薬ジャーナル 0287-4741 47 11 154 159 20111100 read0051714/misc/26553956 ムルヤトノサプタ片岡宏介関根伸一バータルジャフツェルメグ福井誠金川裕子後藤優樹伊藤博夫経鼻粘膜アジュバントCpG ODNはマクロファージのTLR9発現と歯周病原細菌抗原特異的Th1免疫応答を誘導する口腔衛生学会雑誌 0023-2831 61 4 473 null 20110830 read0051714/misc/39496606 福井誠ムルヤトノ・サプタバータルジャフ・ツェルメグ後藤優樹片岡宏介林田秀明川崎浩二前田隆浩中里未央齋藤俊行伊藤博夫 : ヒト唾液中および血中の抗ホスホリルコリン抗体と動脈硬化リスクとの関連性五島研究 (一社)日本口腔衛生学会口腔衛生学会雑誌 0023-2831 61 4 444 444 20110800 read0051714/misc/25743061 片岡宏介 Baatarjav Tselmeg 福井誠藤橋浩太郎伊藤博夫粘膜免疫腸内共生菌と粘膜ワクチン DNA粘膜アジュバントFlt3 Ligandプラスミドは，フォスフォリルコリン特異的T15抗体を誘導する(Induction of PC-specific T15 idiotype Ab in mucosal secretions by Flt3 ligand plasmid as a mucosal adjuvant) (NPO)日本免疫学会日本免疫学会総会・学術集会記録 0919-1984 39 193 193 20091100 read0051714/misc/29117807 Kosuke Kataoka Keiko Fujihashi Shinichi Sekine Ryoki Kobayashi Rebekah Sims Hiroo Ito Jerry R. McGhee Satoshi Shizukuishi Kohtaro Fujihashi Nasal Cholera Toxin Up-regulates Mucosal DCs And B1 B Cells Interactions For The Induction Of Innate IgA CSR. FASEB JOURNAL 0892-6638 22 null null 20080400 read0051714/misc/23616419 Kosuke Kataoka Keiko Fujihashi Shinichi Sekine Tatsuya Fukuiwa Ryoki Kobayashi Hideaki Suzuki Hideki Nagata Kiyoshi Takatsu Satoshi Shizukuishi Jerry R. McGhee Kohtaro Fujihashi Nasal cholera toxin elicits IL-5 and IL-5 receptor alpha-chain expressing B-1a B cells for innate mucosal IgA antibody responses Nasal cholera toxin elicits IL-5 and IL-5 receptor α chain expressing B-1a B cells for innate mucosal IgA antibody responses. JOURNAL OF IMMUNOLOGY Journal of Immunology 0022-1767 178 10 6058 6065 20070500 read0051714/misc/23616392 Miyuki Kibayashi Muneo Tanaka Nobuko Nishida Masae Kuboniwa Kosuke Kataoka Hideki Nagata Kunio Nakayama Kanehisa Morimoto Satoshi Shizukuishi Longitudinal study of the association between smoking as a periodontitis risk and salivary biomarkers related to periodontitis JOURNAL OF PERIODONTOLOGY 0022-3492 78 5 859 867 20070500 10.1902/jop.2007.060292 read0051714/misc/23616386 Kosuke Kataoka Keiko Fujihashi Shinichi Sekine Tatsuya Fukuiwa Ryoki Kobayashi Hideaki Suzuki Hideki Nagata Kiyoshi Takatsu Satoshi Shizukuishi Jerry R. McGhee Kohtaro Fujihashi Nasal cholera toxin elicits IL-5 and IL-5 receptor alpha-chain expressing B-1a B cells for innate mucosal IgA antibody responses JOURNAL OF IMMUNOLOGY 0022-1767 178 10 6058 6065 20070500 read0051714/misc/28814558 Shinichi Sekine Kosuke Kataoka Hideki Asanuma Tatsuya Fukuiwa Julia Davydova Yasuo Adachi Ryoki Kobayashi Keiko Fujihashi Satoshi Shizukuishi Masato Yamamoto Kohtaro Fujihashi NALT CD11b(+) Dendritic Cell Migration Contributes to Induction of Ag Specific Immunity JOURNAL OF IMMUNOLOGY 1550-6606 178 null null 20070400 read0051714/misc/23616424 特集I: IgAの産生をめぐって「コレラトキシンの粘膜投与によるIgA産生B細胞の誘導」科学評論社 null null 20070000 read0051714/misc/23616423 「コレラトキシンの粘膜投与によるIgA産生B細胞の誘導」臨床免疫・アレルギー科 Clinical Immunology & Allergy 47(6):640-646 null null 20070000 read0051714/misc/23616391 Induction of B-1a B cells by cholera toxin as a nasal adjuvant Clinical Immunology & Allergy, null null 20070000 read0051714/misc/23616390 片岡宏介雫石聰藤橋浩太郎 IgAB コレラトキシンの粘膜投与によるIgA産生B細胞の誘導 (特集 IgAの産生をめぐって) 科学評論社 · Clinical Immunology & Allergy 臨床免疫・アレルギー科 1881-1930 47(6):640-646 6 640 646 20070000 read0051714/misc/23616387 Nobuko Nishida Yumiko Yamamoto Muneo Tanaka Kazuhiko Maeda Kosuke Kataoka Kunio Nakayama Kanehisa Morimoto Satoshi Shizukuishi Association between passive smoking and salivary markers related to periodontitis JOURNAL OF CLINICAL PERIODONTOLOGY 0303-6979 33 10 717 723 20061000 10.1111/j.1600-051X.2006.00982.x read0051714/misc/25743064 福岩達哉小林良喜関根伸一片岡宏介 Kreig Arthur M McGhee Jerry R 藤橋浩太郎黒野祐一記憶型樹状細胞を介した長期持続性粘膜免疫を誘導する経鼻アジュバントの開発日本耳鼻咽喉科免疫アレルギー学会耳鼻咽喉科免疫アレルギー 0913-0691 24 2 17 18 20060900 read0051714/misc/23616388 Yukari Hagiwara Yuki I. Kawamura Kosuke Kataoka Bibi Rahima Raymond J. Jackson Katsuhiro Komase Taeko Dohi Prosper N. Boyaka Yoshifumi Takeda Hiroshi Kiyono Jerry R. McGhee Kohtaro Fujihashi A second generation of double mutant cholera toxin adjuvants: Enhanced immunity without intracellular trafficking JOURNAL OF IMMUNOLOGY 0022-1767 177 5 3045 3054 20060900 read0051714/misc/39043974 高橋一郎片岡宏介口腔保健の免疫学的展開口腔衛生学会雑誌 = JOURNAL OF DENTAL HEALTH 0023-2831 56 4 429 432 20060830 read0051714/misc/39043957 野田倫世田中宗雄野中綾片岡宏介雫石聰口臭に対する主観的なストレスと口臭検査値および口腔保健要因との関連性口腔衛生学会雑誌 = JOURNAL OF DENTAL HEALTH 0023-2831 56 4 480 480 20060830 read0051714/misc/23616393 H Nagata Y Inagaki Y Yamamoto K Maeda K Kataoka K Osawa S Shizukuishi Inhibitory effects of macrocarpals on the biological activity of Porphyromonas gingivalis and other periodontopathic bacteria ORAL MICROBIOLOGY AND IMMUNOLOGY 0902-0055 21 3 159 163 20060600 10.1111/j.1399-302X.2006.00269.x read0051714/misc/28890761 Shinichi Sekine Kosuke Kataoka Mika Sasaki Ryoki Kobayashi Keiko Fujihashi Ichiro Saito Satoshi Shizukuishi Jerry R. McGhee Kohtaro Fujihashi Development and characterization of salivary IL-7 transgenic mice JOURNAL OF IMMUNOLOGY 0022-1767 176 S231 S231 20060400 read0051714/misc/23616421 歯周病と全身疾患日本歯科評論 null null 20060000 read0051714/misc/23616385 Yutaka Terao Shigefumi Okamoto Kosuke Kataoka Shigeyuki Hamada Shigetada Kawabata Protective immunity against Streptococcus pyogenes challenge in mice after immunization with fibronectin-binding protein Journal of Infectious Diseases 0022-1899 192 12 2081 2091 20051215 10.1086/498162 16288371 2-s2.0-28844485361 read0051714/misc/23616410 A Hino S Fukuyama K Kataoka MN Kweon K Fujihashi H Kiyono Nasal IL-12p70 DNA prevents and treats intestinal allergic diarrhea JOURNAL OF IMMUNOLOGY 0022-1767 174 11 7423 7432 20050600 read0051714/misc/23616381 R Kobayashi T Kohda K Kataoka H Ihara S Kozaki DW Pascual HF Staats H Kiyono McGhee, JR K Fujihashi A novel neurotoxoid vaccine prevents mucosal botulism JOURNAL OF IMMUNOLOGY 0022-1767 174 4 2190 2195 20050200 read0051714/misc/23616418 Protective immunity against Streptococcus Pyogenes challenge in mice after immunization with fibronectin-binding protein. J. Infect. Dis. 192;2081-2091 null null 20050000 10.1086/498162 read0051714/misc/23616389 Protective immunity against Streptococcus Pyogenes challenge in mice after immunization with fibronectin-binding protein. J. Infect. Dis. 192;2081-2091 null null 20050000 10.1086/498162 read0051714/misc/23616378 N Yoshino FXS Lu K Fujihashi Y Hagiwara K Kataoka D Lu L Hirst M Honda FW van Ginkel Y Takeda CJ Miller H Kiyono McGhee, JR A novel adjuvant for mucosal immunity to HIV-1 gp120 in nonhuman primates JOURNAL OF IMMUNOLOGY 0022-1767 173 11 6850 6857 20041200 read0051714/misc/23616382 K Maeda H Nagata A Nonaka K Kataoka M Tanaka S Shizukuishi Oral streptococcal glyceraldehyde-3-phosphate dehydrogenase mediates interaction with Porphyromonas gingivalis fimbriae MICROBES AND INFECTION 1286-4579 6 13 1163 1170 20041100 10.1016/j.micinf.2004.06.005 read0051714/misc/23616379 Muneo Tanaka Yumiko Yamamoto Masae Kuboniwa Aya Nonaka Nobuko Nishida Kazuhiko Maeda Kosuke Kataoka Hideki Nagata Satoshi Shizukuishi Contribution of periodontal pathogens on tongue dorsa analyzed with real-time PCR to oral malodor Microbes and Infection 1286-4579 6 12 1078 1083 20041000 10.1016/j.micinf.2004.05.021 15380777 2-s2.0-4544287794 read0051714/misc/23616368 M Tanaka Y Yamamoto M Kuboniwa A Nonaka N Nishida K Maeda K Kataoka H Nagata S Shizukuishi Contribution of periodontal pathogens on tongue dorsa analyzed with real-time PCR to oral malodor MICROBES AND INFECTION 1286-4579 6 12 1078 1083 20041000 10.1016/j.micinf.2004.05.021 read0051714/misc/23616367 S Sekine K Kataoka M Tanaka H Nagata T Kawakami K Akaji S Aimoto S Shizukuishi Active domains of salivary statherin on apatitic surfaces for binding to Fusobacterium nucleatum cells MICROBIOLOGY-SGM 1350-0872 150 2373 2379 20040700 10.1099/mic.0.27107-0 read0051714/misc/23616380 M Tanaka H Anguri A Nonaka K Kataoka H Nagata J Kita S Shizukuishi Clinical assessment of oral malodor by the electronic nose system JOURNAL OF DENTAL RESEARCH 0022-0345 83 4 317 321 20040400 read0051714/misc/23616363 K Kataoka McGhee, JR R Kobayashi K Fujihashi S Shizukuishi K Fujihashi Nasal Flt3 ligand cDNA elicits CD11c(+)CD8(+) dendritic cells for enhanced mucosal immunity JOURNAL OF IMMUNOLOGY 0022-1767 172 6 3612 3619 20040300 read0051714/misc/23616415 電子嗅覚装置を利用した口臭構成物質の評価口腔衛生学会雑誌, 54(4), 473, 2004. 54(4), 473,/, null null 20040000 read0051714/misc/23616414 ハイネマン歯科英和辞典4th ed. Butterworth Heinemann null null 20040000 read0051714/misc/23616384 Shinichi Sekine Kosuke Kataoka Muneo Tanaka Hideki Nagata Toru Kawakami Kenichi Akaji Saburo Aimoto Satoshi Shizukuishi Active domains of salivary statherin on apatitic surfaces for binding to Fusobacterium nucleatum cells Society for General Microbiology Microbiology 1350-0872 150 7 2373 2379 20040000 10.1099/mic.0.27107-0 15256578 2-s2.0-4344623485 read0051714/misc/23616383 HEINEMANN DENTAL DICTIONARY Butterworth Heinemann null null 20040000 read0051714/misc/23616366 M. Tanaka H. Anguri A. Nonaka K. Kataoka H. Nagata J. Kita S. Shizukuishi Clinical assessment of oral malodor by the electronic nose system SAGE Publications Inc. Journal of Dental Research 0022-0345 83 4 317 321 20040000 10.1177/154405910408300409 15044506 2-s2.0-2342537824 read0051714/misc/28559211 K. Fujihashi K. Kataoka R. Kobayashi T. Ogawa J. R. McGhee K. Fujihashi Preferential induction of TLR4 expression on gamma 8 T-cells of submandibular glands. JOURNAL OF DENTAL RESEARCH 0022-0345 82 B379 B379 20030600 read0051714/misc/28535500 K. Kataoka D. M. Pittman R. Kobayashi K. Fujihashi J. R. Mcghee K. Fujihashi Nasal cholera toxin enhances innate mucosal immune responses. JOURNAL OF DENTAL RESEARCH 0022-0345 82 B218 B218 20030600 read0051714/misc/38232923 R Kobayashi M Zemlin K Kataoka C Zemlin R Schelonka R Dimmitt A Hino K Fujihashi HW Schroeder McGhee, JR K Fujihashi Oral live r-Salmonella expressing fragment C of tetanus toxin induces antigen-specific IgA responses in mice with limited IgH variable region diversity FASEB JOURNAL 0892-6638 17 7 C85 C85 20030400 read0051714/misc/28548520 CM Zemlin M Zemlin R Kobayashi K Kataoka Gohma, I McGhee, JR K Fujihashi HW Schroeder TH1 dependent selection of the mucosal IgA repertoire is unable to overcome the contribution of an altered germline repertoire FASEB JOURNAL 0892-6638 17 7 C190 C190 20030400 read0051714/misc/28547115 K Kataoka R Kobayashi A Hino K Fujihashi McGhee, JR K Fujihashi Nasal Flt3 ligand plasmid induces antigen-specific immune responses by mucosal dendritic cell recruitment FASEB JOURNAL 0892-6638 17 7 C320 C320 20030400 read0051714/misc/23616365 H Kato K Fujihashi R Kato T Dohi K Fujihashi Y Hagiwara K Kataoka R Kobayashi McGhee, JR Lack of oral tolerance in aging is due to sequential loss of Peyer's patch cell interactions INTERNATIONAL IMMUNOLOGY 0953-8178 15 2 145 158 20030200 10.1093/intimm/dxg011 read0051714/misc/23616364 Y Hagiwara McGhee, JR K Fujihashi R Kobayashi N Yoshino K Kataoka Y Etani MN Kweon S Tamura T Kurata Y Takeda H Kiyono K Fujihashi Protective mucosal immunity in aging is associated with functional CD4(+) T cells in nasopharyngeal-associated lymphoreticular tissue JOURNAL OF IMMUNOLOGY 0022-1767 170 4 1754 1762 20030200 read0051714/misc/28586711 S Sekine K Kataoka S Shizukuishi Identification of binding sites of statherin to Fusobacterium nucleatum. JOURNAL OF DENTAL RESEARCH 0022-0345 81 A114 A114 20020300 read0051714/misc/38098112 Sekine Shin-ichi 関根伸一 Kataoka Kosuke 片岡宏介 Shuzukuishi Satoshi 雫石聰 Binding Domains of Salivary Tyrosine-Rich Protein (Statherin) for Fusobacterium nucleatum B-19-14 : 50 Fusobacterium nucleatum菌体に対する唾液高チロシンタンパク質スタセリンの結合領域 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 43 137 137 20010330 read0051714/misc/39238389 関根伸一片岡宏介雫石聰 Fusobacterium nucleatumと唾液タンパク質スタセリンとの結合口腔衛生学会雑誌 0023-2831 50 4 588 589 20000900 read0051714/misc/23616401 A Amano Nakagawa, I K Kataoka Morisaki, I S Hamada Distribution of Porphyromonas gingivalis strains with fimA genotypes in periodontitis patients Distribution of Porphyromonas gingivalis Strains with fimA Genotypes in Periodontitis Patients(共著) JOURNAL OF CLINICAL MICROBIOLOGY Journal of Clinical Microbiology 0095-1137 37 5 1426 1430 19990500 read0051714/misc/23616376 Distribution of Porphyromonas gingivalis Strains with fimA Genotypes in Periodontitis Patients(共著) Journal of Clinical Microbiology 37 5 1426 1430 19990000 read0051714/misc/23616369 Secretion of Functional Salivary Peptide by Streptococcus gordonii Which Inhibits Fimbria-Mediated Adhesion of Porphyromonas gingivalis(共著) Infection and Immunity 67 8 3780 3785 19990000 read0051714/misc/28472813 A Amano T Nakamura K Kataoka S Hamada S Shizukuishi Morisaki, I Binding of salivary proline-rich glycoprotein/protein to Porphyromonas gingivalis fimbriae via common active domain. JOURNAL OF DENTAL RESEARCH 0022-0345 77 139 139 19980000 read0051714/misc/28846778 M Kuboniwa A Amano K Kataoka S Shizukuishi Purification of hemoglobin binding protein isolated from Porphyromonas gingivalis JOURNAL OF DENTAL RESEARCH 0022-0345 76 5 1174 1174 19970500 read0051714/misc/28852827 K Kataoka A Amano M Kuboniwa H Horie S Shizukuishi Active sites of salivary proline-rich protein that mediate binding to Porphyromonas gingivalis fimbriae JOURNAL OF DENTAL RESEARCH 0022-0345 76 1669 1669 19970000 read0051714/misc/38089872 HANIOKA Takashi 埴岡隆 TANAKA Muneo 田中宗雄 KISHIMOTO Mikako 岸本美香子 KATAOKA Kosuke 片岡宏介 SHIZUKUISHI Satoshi 雫石聰 Partitional Measurement of Gingival Blood Flow by Laser-Doppler-Flowmeter Using Probe with Different Optic Fiber Separations C-14-11 : 20 デュアルピッチ・レーザープローブによる歯肉血流量の分画測定 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 38 145 145 19960905 read0051714/misc/26539697 Amano Atsuo 天野敦雄 Kuboniwa Masae 久保庭雅恵 Horie Hiroshi 堀江博 Kataoka Kosuke 片岡宏介 Shizukuishi Satoshi 雫石聰 Effect of Temperature Stress on Adherence Abilities and Antigenicity of Porphyromonas gingivalis A-23-9 : 50 温度ストレスがPorphyromonas gingivalisの付着能および抗原性に及ぼす影響 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 38 0 102 102 19960905 read0051714/misc/23616407 AMANO Atsuo 天野敦雄 KUBONIWA Masae 久保庭雅恵 HORIE Hiroshi 堀江博 KATAOKA Kosuke 片岡宏介 NAGATA Hideki 永田英樹 SHIZUKUISHI Satoshi 雫石聰 Effects of Temperature Stress on Adhesive Abilities and Antigenicity of Porphyromonas gingivalis Effects of temperature stress on Adhesive abilities and Antigenecity of Porphyromonas gingivalis Periodontopathic bacteria are able to survive various changes of their environment due to the undergoing advances of periodontal diseases. There are mechanisms which regulate the alterna-tion of bacterial metabolism and protein expression to adapt physiological functions to environmental stresses due to changes of temperature, pH ratio and nutrients. In this study, we examined the effects of a 2°C elevation of temperature on adhe-sive abilities and antigenicity of Porphyromonas gingivalis. P. gingivalis ATCC 33277 was grown at 37°C to the stationary phase. Inoculums (1%) from the culture were further incubated at 37°C, and 39°C to investigate stress due to elevation in temperature in the stationary phase. There was no difference in the growth curves of cells at 37°C and 39°C. However, observations of cell surfaces by an electron microscope revealed that cells grown at 39°C expressed little filamentous structure of fim-briae which can be observed in 37°C-cells. 39°C-cells significantly lost binding abilities to hydroxy-apatite beads (HAP) coated with whole saliva and their specific salivary receptors, proline-rich pro-tein and statherin. Coaggregation activity of 39°C-cells with Streptococcus oralis was reduced by 92% of that of 37°C-cells. Whole cells at 37 and 39°C were loaded to SDS-PAGE for western blot analysis and were probed with marginal peri-odontitis-patient serum. Major reaction bands of 37°C-cells were detected at 75, 43, 40, 34, and 29 kDa positions. In the profile of probed bands of 39°C-cells, densities of these major bands were significantly reduced, and several new bands were observed with a wide range of molecular sizes. These observations suggested that P. gingivalis may undergo alterations in its physiological functions and antigenicity as a result of a change of tempera-ture which can be found with advances of several types of periodontal diseases. 歯周病原性菌の歯周組織への定着, 炎症の惹起, 組織破壊と推移する辺縁性歯周炎の進行過程において, 病原性菌の存在する環境変化のひとつとして局所の急性炎症による歯周組織の温度上昇が考えられる．Porphyromonas gingivalis ATCC 33277株を39℃で培養し2℃の温度ストレスを加えると, 37℃培養に比べ菌体表層の線毛構造物の発現が著しく抑制されるとともに, ヒト全唾液被覆ハイドロキシアパタイトビーズ (HAP) へのP. gingivalisの付着能が著しく減少した．P. gingivalis線毛と特異的に結合する唾液タンパク質である高プロリンタンパク質およびスタセリンで被覆したHAPと菌体との結合も顕著な減少を示した．さらに, 本菌のStreptococcus oralisとの共凝集能も92%の減少を示した．全菌体抽出物のウエスタンブロットと歯周炎患者血清との反応バンドにおいて37℃と39℃培養菌の間に差が認められ239℃培養菌において既存バンドの濃度の減少と新しいバンドの検出が観察され2両菌体の血清との反応性には相違が認められた．以上の結果から, P. gingivalisは歯周炎の進行に伴う周囲環境の変化に対応して, その付着能や抗原性を変化させていく可能性が示唆され, 本菌の歯周ポケット内での生態の一端が推測された． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Journal of the Japanese Society of Periodontology 0385-0110 39 1 86 92 19960000 10.2329/perio.39.86 read0051714/misc/23616403 温度ストレスがポルフィロモナス・ジンジバリスの付着能および抗原性に及ぼす影響(共著) 日本歯周病学会会誌 39 1 86 92 19960000 read0051714/misc/23616402 Binding sites of Salivary Statherin for Porphyromonas gingivalis Recombinant Fimbrillin Infection and Immunity 64 10 4249 4254 19960000 read0051714/misc/23616374 Binding sites of Salivary Statherin for Porphyromonas gingivalis Recombinant Fimbrillin(共著) Infection and Immunity 64 10 4249 4254 19960000 read0051714/misc/23616373 Atsuo Amano 天野敦雄 Masae Kuboniwa 久保庭雅恵 Hiroshi Horie 堀江博 Kosuke Kataoka 片岡宏介 Hideki Nagata 永田英樹 Satoshi Shizukuishi 雫石聰 Effects of temperature stress on Adhesive abilities and Antigenecity of Porphyromonas gingivalis 温度ストレスがPorphyromonas gingivalis の付着能および抗原性に及ぼす影響 Periodontopathic bacteria are able to survive various changes of their environment due to the undergoing advances of periodontal diseases. There are mechanisms which regulate the alterna-tion of bacterial metabolism and protein expression to adapt physiological functions to environmental stresses due to changes of temperature, pH ratio and nutrients. In this study, we examined the effects of a 2°C elevation of temperature on adhe-sive abilities and antigenicity of Porphyromonas gingivalis. P. gingivalis ATCC 33277 was grown at 37°C to the stationary phase. Inoculums (1%) from the culture were further incubated at 37°C, and 39°C to investigate stress due to elevation in temperature in the stationary phase. There was no difference in the growth curves of cells at 37°C and 39°C. However, observations of cell surfaces by an electron microscope revealed that cells grown at 39°C expressed little filamentous structure of fim-briae which can be observed in 37°C-cells. 39°C-cells significantly lost binding abilities to hydroxy-apatite beads (HAP) coated with whole saliva and their specific salivary receptors, proline-rich pro-tein and statherin. Coaggregation activity of 39°C-cells with Streptococcus oralis was reduced by 92% of that of 37°C-cells. Whole cells at 37 and 39°C were loaded to SDS-PAGE for western blot analysis and were probed with marginal peri-odontitis-patient serum. Major reaction bands of 37°C-cells were detected at 75, 43, 40, 34, and 29 kDa positions. In the profile of probed bands of 39°C-cells, densities of these major bands were significantly reduced, and several new bands were observed with a wide range of molecular sizes. These observations suggested that P. gingivalis may undergo alterations in its physiological functions and antigenicity as a result of a change of tempera-ture which can be found with advances of several types of periodontal diseases. 歯周病原性菌の歯周組織への定着, 炎症の惹起, 組織破壊と推移する辺縁性歯周炎の進行過程において, 病原性菌の存在する環境変化のひとつとして局所の急性炎症による歯周組織の温度上昇が考えられる．Porphyromonas gingivalis ATCC 33277株を39℃で培養し2℃の温度ストレスを加えると, 37℃培養に比べ菌体表層の線毛構造物の発現が著しく抑制されるとともに, ヒト全唾液被覆ハイドロキシアパタイトビーズ (HAP) へのP. gingivalisの付着能が著しく減少した．P. gingivalis線毛と特異的に結合する唾液タンパク質である高プロリンタンパク質およびスタセリンで被覆したHAPと菌体との結合も顕著な減少を示した．さらに, 本菌のStreptococcus oralisとの共凝集能も92%の減少を示した．全菌体抽出物のウエスタンブロットと歯周炎患者血清との反応バンドにおいて37℃と39℃培養菌の間に差が認められ239℃培養菌において既存バンドの濃度の減少と新しいバンドの検出が観察され2両菌体の血清との反応性には相違が認められた．以上の結果から, P. gingivalisは歯周炎の進行に伴う周囲環境の変化に対応して, その付着能や抗原性を変化させていく可能性が示唆され, 本菌の歯周ポケット内での生態の一端が推測された． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Society of Periodontology 日本歯周病学会会誌 0385-0110 39 1 86 92 19960000 10.2329/perio.39.86 read0051714/misc/23616405 A AMANO AMANO A M KUBONIWA KUBONIWA M K KATAOKA KATAOKA K K TAZAKI TAZAKI K E INOSHITA INOSHITA E H NAGATA NAGATA H H TAMAGAWA TAMAGAWA H S SHIZUKUISHI SHIZUKUISHI S BINDING OF HEMOGLOBIN BY PORPHYROMONAS-GINGIVALIS Binding of hemoglobin by Porphyromonas gingivalis(共著) FEMS MICROBIOLOGY LETTERS FEMS Microbiology Letters 0378-1097 134 1 63 67 19951200 10.1016/0378-1097(95)00382-F 8593957 read0051714/misc/23616371 Atsuo Amano Masae Kuboniwa Kousuke Kataoka Katsuko Tazaki Eiji Inoshita Hideki Nagata Hiroo Tamagawa Satoshi Shizukuishi Binding of hemoglobin by Porphyromonas gingivalis FEMS Microbiology Letters 1574-6968 134 1 63 67 19951201 10.1016/0378-1097(95)00382-F 8593957 2-s2.0-0028879773 read0051714/misc/38386861 埴岡隆片岡宏介田中宗雄雫石聡西日本3大学の歯学生の喫煙行動および喫煙と健康に関する意識調査口腔衛生学会雑誌 0023-2831 45 4 576 577 19950930 read0051714/misc/38093510 AMANO Atsuo 天野敦雄 KATAOKA Kosuke 片岡宏介 SHIZUKUISHI Satoshi 雫石聰 Relationship between the Binding and Availability of Transferrin for Growth by Porphyromonas gingivalis C-1 Porphyromonas gingivalisのトランスフェリンとの結合性とその増殖への利用能との関連性 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 37 153 153 19950906 read0051714/misc/38093476 KATAOKA Kosuke 片岡宏介 AMANO Atsuo 天野敦雄 SHIZUKUISHI Satoshi 雫石聰 Salivary Prolin-Rich-Protein Domains Involved in Binding to Porphyromonas gingivalis Fimbrillin B-53-14 : 00 Porphyromonas gingivalis線毛に対する唾液高プロリンタンパク質の結合領域 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 37 143 143 19950906 read0051714/misc/23616406 S SHIZUKUISHI SHIZUKUISHI S K TAZAKI TAZAKI K E INOSHITA INOSHITA E K KATAOKA KATAOKA K T HANIOKA HANIOKA T A AMANO AMANO A EFFECT OF CONCENTRATION OF COMPOUNDS CONTAINING IRON ON THE GROWTH OF PORPHYROMONAS-GINGIVALIS Effect of concentration of compounds containing iron on the growth of Porphyromonas gingivalis(共著) FEMS MICROBIOLOGY LETTERS FEMS Microbiology Letters 0378-1097 131 3 313 317 19950900 10.1016/0378-1097(95)00277-C 7557343 read0051714/misc/23616372 S SHIZUKUISHI K TAZAKI E INOSHITA K KATAOKA T HANIOKA A AMANO EFFECT OF CONCENTRATION OF COMPOUNDS CONTAINING IRON ON THE GROWTH OF PORPHYROMONAS-GINGIVALIS FEMS MICROBIOLOGY LETTERS 0378-1097 131 3 313 317 19950900 10.1016/0378-1097(95)00277-C 7557343 read0051714/misc/28762652 K TAZAKI K KATAOKA S SHIZUKUISHI BINDING OF HUMAN HEMOGLOBIN BY PORPHYROMONAS-GINGIVALIS JOURNAL OF DENTAL RESEARCH 0022-0345 74 3 974 974 19950300 read0051714/misc/28759354 S SHIZUKUISHI K KATAOKA K TAZAKI A AMANO EFFECT OF IRON-CONTAINING COMPOUNDS ON THE GROWTH OF PORPHYROMONAS-GINGIVALIS JOURNAL OF DENTAL RESEARCH 0022-0345 74 504 504 19950000 read0051714/misc/23616404 H NAGATA A AMANO M OJIMA M TANAKA K KATAOKA S SHIZUKUISHI EFFECT OF BINDING OF FIBRINOGEN TO EACH BACTERIUM ON COAGGREGATION BETWEEN PORPHYROMONAS-GINGIVALIS AND STREPTOCOCCUS-ORALIS Effect of binding of fibrinogen to each bacterium on coaggregation between Porphyromonas gingivalis and Streptococcus oralis(共著) ORAL MICROBIOLOGY AND IMMUNOLOGY Oral Microbiology and Immunology 0902-0055 9 6 359 363 19941200 read0051714/misc/23616370 Effect of binding of fibrinogen to each bacterium on coaggregation between Porphyromonas gingivalis and Streptococcus oralis(共著) Oral Microbiology and Immunology 9 359 363 19940000 read0051714/presentations/53881934 Mio Kitamura Kei Tanaka Misaki Ichinomiya Takaharu Goto Kosuke Kataoka Yasuhiko Shirayama Shinji Fujiwara Washi tape (Masking Tape) Art as Social and Cultural Prescribing in a Rural Area Facing Depopulation: A Protocol Study Focusing on Curiosity and Ikigai 京都大学国際シンポジウム社会的処方・文化的処方国際会議(ISPC2026) 20260527 read0051714/presentations/53881919 大矢朋慧一ノ宮実咲 Walid Ahmed 三木かなめ須磨紫乃山下和彦片岡宏介天然素材 -キチンナノファイバー(bCNF)の経鼻投与による粘膜および全身免疫応答の賦活化第75回日本口腔衛生学会学術大会 20260523 read0051714/presentations/53881910 須磨紫乃三木かなめ一ノ宮実咲北村美渚片岡宏介高齢者における口腔の健康と主観的・客観的ストレス評価との関連第75回日本口腔衛生学会学術大会 20260523 read0051714/presentations/53881898 一ノ宮実咲北村美渚須磨紫乃片岡宏介過疎地域及び市街地に共住する高齢者における社会的フレイルと口腔・健康状態の多面的比較第75回日本口腔衛生学会学術大会 20260523 read0051714/presentations/53881886 Walid A. Abdelnaser Kaname Miki Tomoe Oya Misaki Ichinomiya Shino Suma Eiji Tanaka Kosuke Kataoka Comparative antibacterial activity of citrus essential oils against Lactobacillus casei 第67回例会四国歯学会 20260305 read0051714/presentations/53881875 片岡宏介元家由香梨三木かなめ大矢朋慧渡辺あかり須磨紫乃柑橘系精油によるう蝕原因菌増殖に与える影響-唾液中のう蝕原因菌コントロールに向けて- 第4回日本唾液ケア科学会 20251123 read0051714/presentations/53881857 一ノ宮実咲須磨紫乃大矢朋慧片岡宏介過疎地域在住高齢者におけるオーラルヘルスリテラシーの実態と口腔衛生行動および口腔の健康状態との関連第36回近畿・中国・四国口腔衛生学会総会 20251005 read0051714/presentations/53881840 元家由加梨三木かなめ大矢朋彗渡辺朱理須磨紫乃片岡宏介香り柑橘系精油を用いたStreptococcus mutansの抗菌効果について第36回近畿・中国・四国口腔衛生学会総会 20251005 read0051714/presentations/53881830 渡辺朱理三木かなめ須磨紫乃片岡宏介松山美和口腔内細菌へのオゾン水の殺菌力効果について第36回近畿・中国・四国口腔衛生学会総会 20251005 read0051714/presentations/53881816 一ノ宮実咲須磨紫乃片岡宏介徳島県木屋平地区における高齢者のオーラルヘルスリテラシーと社会的フレイルとの関連第25回日本歯科医学会学術大会 20250926 read0051714/presentations/53881804 政ケ谷恒暉須磨紫乃三木かなめ山下喜久片岡宏介サークル・バイブレーション法の歯垢除去効果と疲労感を検証するための予備的研究第25回日本歯科医学会学術大会 20250926 read0051714/presentations/50609878 Misaki Ichinomiya Shino Suma Kosuke Kataoka Relationship between social frailty and oral health literacy 103th General session and exhibition of the IADR 20250627 read0051714/presentations/50609876 Hitomi Niki Daisuke Hinode Makoto Fukui Ichika Sone Kosuke Kataoka Moneno Takeshita Keisuke Kato Effect of toothbrush with branched filament ends on dental plaque 103th General session and exhibition of the IADR 20250627 read0051714/presentations/50609881 竹下萌乃仁木希美曽根一華福井誠片岡宏介日野出大輔加藤啓介分岐毛先歯ブラシのデンタルプラーク除去および歯肉への負荷に対する力学特性評価および臨床研究第68回春季日本歯周病学会学術大会 20250524 read0051714/presentations/50609875 Mio Kitamura Takaharu Goto Misaki Ichinomiya Shinji Fujiwara Kei Tanaka Kosuke Kataoka Yasuhiko Shirayama Literature Review for the Development of the CIH (Curiosity-IKIGAI-Health) Advancement Model 13th IAGG Master Class on Ageing, The Japan Geriatrics Society 20250523 read0051714/presentations/50609900 政ケ谷恒暉須磨紫乃三木かなめ山下喜久片岡宏介サークル・バイブレーション法の歯垢除去効果と疲労感を検証するための予備的研究第74回日本口腔衛生学会学術大会 20250518 read0051714/presentations/50609893 Dara Sari Arini Misaki Ichinomiya Kosuke Kataoka Yoshizo Matsuka Exploring Curcumin's Role in Alleviating Trigeminal Neuropathic Pain 第74回日本口腔衛生学会学術大会 20250518 read0051714/presentations/50609889 栁沢志津子吉岡昌美片岡宏介施設介護職員からみた介護保険施設における歯科専門職との連携第74回日本口腔衛生学会学術大会 20250518 read0051714/presentations/50609886 片岡宏介今から思えばナッジ理論!?-スポーツクラブコーチとしての経験から- 第74回日本口腔衛生学会学術大会シンポジウム5 20250518 read0051714/presentations/50609905 片岡宏介歯科保健の動向-歯科疾患と歯科保健対策の現状- 歯周病と糖尿病海南歯科医師会学術講演会 20250322 read0051714/presentations/50609909 山路梨紗，小松崎凜，藤田真里杏，松本望愛，吉田沙紀，政ケ谷恒暉，須磨紫乃，三木かなめ，片岡宏介サークル・バイブレーション法による歯垢除去効果検証のための予備的実験第65回例会四国歯学会 20250320 read0051714/presentations/50609911 須磨紫乃釜野桜子片岡宏介渡邊毅石津将 for he J-MICC Study Group 大豆食品摂取量が全死因死亡・死因別死亡に与える影響:J-MICC Study 第35回日本疫学会学術総会 20250212 read0051714/presentations/50609918 Kosuke Kataoka Shino Suma Misaki Ichinomiya Tomoko Sumitomo Shigetada Kawabata D-Psicose, a Rare Sugar Down-regulate Development of Candida albicans Hyphae 18th International Union of Microbiological Societies Congress (IUMS) 20241024 read0051714/presentations/50609927 須磨紫乃古田美智子三木かなめ片岡宏介竹下徹口腔の健康とストレスとの関連に関する予備的研究第35回近畿・中国・四国口腔衛生学会総会 20240929 read0051714/presentations/50609925 ダラサリアリニ一ノ宮実咲片岡宏介松香芳三ラット三叉神経痛モデルにおけるクルクミン全身投与効果第35回近畿・中国・四国口腔衛生学会総会 20240929 read0051714/presentations/47368975 Kataoka K Suma S Ichinomiya M Yoshimatsu H Fujihashi K Nasal administration with double DNA adjuvant and phosphorylcholine leads intraperitoneal CD11b-expressing dendritic cells and regulatory T cells. 21st International Congress of Mucosal Immunology 20240708 read0051714/presentations/47368991 仁木希美曽根一華福井誠片岡宏介竹下萌乃加藤啓介日野出大輔デンタルプラークに対する分岐毛を活用した歯ブラシの有効性の検討第73回日本口腔衛生学会学術大会 20240511 read0051714/presentations/47368984 吉松英樹釜田さゆり坂本由紀子加納圭太河村佳穂里土居貴士片岡宏介三宅達郎天然生薬ウコン含有クルクミンがStreptococcus mutansの生育およびバイオフィルム形成に及ぼす影響第73回日本口腔衛生学会学術大会 20240511 read0051714/presentations/47368981 天野由子福井誠日野出大輔柳沢志津子片岡宏介新規経鼻アジュバントによる新型コロナウイルス4株表面タンパク光源に対する免疫応答の差異第73回日本口腔衛生学会学術大会 20240511 read0051714/presentations/47368994 片岡宏介研究者のための論文の書き方講習ミニシンポジウム -今日から使える質向上のためのイロハとは-PART VI 第73回日本口腔衛生学会・総会 20240510 read0051714/presentations/47369000 片岡宏介糖尿病と口腔疾患徳島県医師会糖尿病療養指導士(LCDE)新規認定研修会 20240301 read0051714/presentations/47369009 吉松英樹片岡宏介三宅達郎藤橋浩太郎 Nasal double DNA adjuvant system induces CD11b-positive dendritic cells and regulatory T cells in ApoE KO mice. 第52回日本免疫学会総会 20240118 read0051714/books_etc/36365820 川端, 重忠小松澤, 均大原, 直也寺尾, 豊口腔微生物学・免疫学医歯薬出版 20211200 read0051714/books_etc/36365821 片岡宏介アルカリ性の飲み物を頻回に飲めば，う蝕のリスクは高まらない? 20210600 read0051714/books_etc/13852373 片岡宏介歯科衛生士テキスト口腔衛生学第4版学健書院 20180300 read0051714/books_etc/13839441 相川友直飯田征二大倉正也小島美樹片岡宏介久保庭雅恵倉田秀竹村元秀田中宗雄中川新二永田英樹中原寛和西口隆彦浜口裕弘林直治平賀敏人前田和彦森谷正之ハイネマン歯科英和辞典第2版医歯薬出版会社 20040000 片岡宏介徳島大学 2025年度徳島大学教養教育賞 20250400 兵庫県令和6年度成長産業育成のための研究開発支援事業「応用研究ステージ」天然素材キチンの経鼻投与型新規ワクチンアジュバントへの開発と実用化に向けて 20240700 藤原奈津美片岡宏介徳島大学学長裁量経費厚労省歯科衛生士リカレント事業採択を目指したスーパーデンタルハイジニスト(歯科衛生士界を牽引するリーダー)育成プログラムの実施 20240400 公益財団法人ひょうご科学技術協会ヤエガキ発酵技研株式会社兵庫県令和5年度成長産業育成のための研究開発支援事業「可能性調査・研究」「ベータ-キチンナノファイバーの経鼻投与型ワクチンアジュバントへの応用可能性の調査研究」 20230900 三木康楽会康楽賞 20220200 片岡宏介大阪歯科大学グッドティーチャーノミネート賞 20210200 KATAOKA Kosuke 片岡宏介 WILEY TOP DOWNLOAD ARTICLE2017-2018 20180400 片岡宏介日本歯科医学会歯科医学を中心とした総合的な研究を推進する集い優秀発表賞 20170800 片岡宏介徳島大学歯学部優秀教育賞 20110800 片岡宏介日本口腔衛生学会ライオンアワード(学会賞) 20071000 Society for Mucosal Immunology Young Investigator Award 20050000 片岡宏介大阪大学課外活動奨励賞 20010400