{"insert":{"user_id":"R000041357","type":"published_papers","id":"53222787"},"force":{"see_also":[{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=462284","label":"url"}],"paper_title":{"en":"Porphyromonas gingivalis Outer Membrane Vesicles-Associated DNA Triggers Inflammation by Inducing IL-6 in Astrocytes","ja":"Porphyromonas gingivalis Outer Membrane Vesicles-Associated DNA Triggers Inflammation by Inducing IL-6 in Astrocytes"},"authors":{"en":[{"name":"Takai Ayu"},{"name":"Yoshida Kaya"},{"name":"Hiroshima Yuka"},{"name":"Ikuta Ayu"},{"name":"Seyama Mariko"},{"name":"Uemura Yuta"},{"name":"Yumoto Hiromichi"},{"name":"Ozaki Kazumi"}],"ja":[{"name":"髙井 彩有"},{"name":"吉田 賀弥"},{"name":"廣島 佑香"},{"name":"生田 あゆ"},{"name":"瀬山 真莉子"},{"name":"植村 勇太"},{"name":"湯本 浩通"},{"name":"尾崎 和美"}]},"publication_date":"2026-04-01","publication_name":{"en":"Molecular Oral Microbiology","ja":"Molecular Oral Microbiology"},"languages":["eng"],"referee":true,"identifiers":{"doi":["10.1111/omi.70030"],"issn":["2041-1014"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
{"insert":{"user_id":"R000041357","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://tokushima-u.repo.nii.ac.jp/records/2011697","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/36790046","label":"url"},{"@id":"https://www.scopus.com/pages/publications/85149959736","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=394014","label":"url"}],"paper_title":{"en":"Utility of a haemoglobin test of gingival crevicular fluid: A multicentre, observational study.","ja":"Utility of a haemoglobin test of gingival crevicular fluid: A multicentre, observational study."},"authors":{"en":[{"name":"Ito Hiroshi"},{"name":"Numabe Yukihiro"},{"name":"Hashimoto Shuichi"},{"name":"Sekino Satoshi"},{"name":"Murakashi Etsuko"},{"name":"Ishiguro Hitomi"},{"name":"Sasaki Daisuke"},{"name":"Yaegashi Takashi"},{"name":"Takai Hideki"},{"name":"Mezawa Masaru"},{"name":"Ogata Yorimasa"},{"name":"Watanabe Hisashi"},{"name":"Izumi Yuichi"},{"name":"Kido Jun-ichi"},{"name":"Hiroshima Yuka"},{"name":"Nagata Toshihiko"}],"ja":[{"name":"Ito Hiroshi"},{"name":"Numabe Yukihiro"},{"name":"Hashimoto Shuichi"},{"name":"Sekino Satoshi"},{"name":"Murakashi Etsuko"},{"name":"Ishiguro Hitomi"},{"name":"Sasaki Daisuke"},{"name":"Yaegashi Takashi"},{"name":"Takai Hideki"},{"name":"Mezawa Masaru"},{"name":"Ogata Yorimasa"},{"name":"Watanabe Hisashi"},{"name":"Izumi Yuichi"},{"name":"木戸 淳一"},{"name":"廣島 佑香"},{"name":"永田 俊彦"}]},"publication_date":"2024-04","publication_name":{"en":"Oral Diseases","ja":"Oral Diseases"},"volume":"30","number":"3","starting_page":"1533","ending_page":"1542","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1111/odi.14536"],"issn":["1601-0825"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
{"insert":{"user_id":"R000041357","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://tokushima-u.repo.nii.ac.jp/records/2011878","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/38502469","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=405032","label":"url"}],"paper_title":{"en":"Synthesis of secretory leukocyte protease inhibitor using cell-free protein synthesis system","ja":"Synthesis of secretory leukocyte protease inhibitor using cell-free protein synthesis system"},"authors":{"en":[{"name":"Hiroshima Yuka"},{"name":"Kido Rie"},{"name":"Kido Jun-ichi"},{"name":"Bandou Mika"},{"name":"Yoshida Kaya"},{"name":"Murakami Akikazu"},{"name":"Shinohara Yasuo"}],"ja":[{"name":"廣島 佑香"},{"name":"木戸 理恵"},{"name":"木戸 淳一"},{"name":"板東 美香"},{"name":"吉田 賀弥"},{"name":"村上 明一"},{"name":"篠原 康雄"}]},"description":{"en":"Secretory leukocyte protease inhibitor (SLPI) functions as a protease inhibitor that modulates excessive proteolysis in the body, exhibits broad-spectrum antimicrobial activity, regulates inflammatory responses, and plays an important role in the innate immunity. The purpose of the study was to artificially synthesize a SLPI, an antimicrobial peptide, and investigate its effect on antimicrobial activity against Porphyromonas gingivalis and interleukin-6 (IL-6) production. SLPI protein with a molecular weight of approximately 13 kDa was artificially synthesized using a cell-free protein synthesis (CFPS) system and investigated by western blotting and enzyme-linked immunosorbent assay (ELISA). Disulfide bond isomerase in the protein synthesis mixture increased the amount of SLPI synthesized. The synthesized SLPI (sSLPI) protein was purified and its antimicrobial activity was investigated based on the growth of Porphyromonas gingivalis and bacterial adhesion to oral epithelial cells. The effect of sSLPI on IL-6 production in human periodontal ligament fibroblasts (HPLFs) was examined by ELISA. Our results showed that sSLPI significantly inhibited the growth of Porphyromonas gingivalis and bacterial adhesion to oral epithelial cells and further inhibited IL-6 production by HPLFs. These results suggested that SLPI artificially synthesized using the CFPS system may play a role in the prevention of periodontal diseases through its antimicrobial and anti-inflammatory effects.","ja":"Secretory leukocyte protease inhibitor (SLPI) functions as a protease inhibitor that modulates excessive proteolysis in the body, exhibits broad-spectrum antimicrobial activity, regulates inflammatory responses, and plays an important role in the innate immunity. The purpose of the study was to artificially synthesize a SLPI, an antimicrobial peptide, and investigate its effect on antimicrobial activity against Porphyromonas gingivalis and interleukin-6 (IL-6) production. SLPI protein with a molecular weight of approximately 13 kDa was artificially synthesized using a cell-free protein synthesis (CFPS) system and investigated by western blotting and enzyme-linked immunosorbent assay (ELISA). Disulfide bond isomerase in the protein synthesis mixture increased the amount of SLPI synthesized. The synthesized SLPI (sSLPI) protein was purified and its antimicrobial activity was investigated based on the growth of Porphyromonas gingivalis and bacterial adhesion to oral epithelial cells. The effect of sSLPI on IL-6 production in human periodontal ligament fibroblasts (HPLFs) was examined by ELISA. Our results showed that sSLPI significantly inhibited the growth of Porphyromonas gingivalis and bacterial adhesion to oral epithelial cells and further inhibited IL-6 production by HPLFs. These results suggested that SLPI artificially synthesized using the CFPS system may play a role in the prevention of periodontal diseases through its antimicrobial and anti-inflammatory effects."},"publication_date":"2024-02","publication_name":{"en":"Odontology","ja":"Odontology"},"volume":"112","number":"4","starting_page":"1103","ending_page":"1112","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1007/s10266-024-00910-8"],"issn":["1618-1255"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
{"insert":{"user_id":"R000041357","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"http://repo.lib.tokushima-u.ac.jp/118518","label":"url"},{"@id":"https://tokushima-u.repo.nii.ac.jp/records/2011250","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/36266256","label":"url"},{"@id":"https://cir.nii.ac.jp/crid/1050867133848428672/","label":"url"},{"@id":"https://www.scopus.com/pages/publications/85141542224","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=393137","label":"url"}],"paper_title":{"en":"Porphyromonas gingivalis outer membrane vesicles in cerebral ventricles activate microglia in mice","ja":"Porphyromonas gingivalis outer membrane vesicles in cerebral ventricles activate microglia in mice"},"authors":{"en":[{"name":"Yoshida Kayo"},{"name":"Yoshida Kaya"},{"name":"Seyama Mariko"},{"name":"Hiroshima Yuka"},{"name":"Mekata Mana"},{"name":"Fujiwara Natsumi"},{"name":"Kudo Yasusei"},{"name":"Ozaki Kazumi"}],"ja":[{"name":"吉田 佳世"},{"name":"吉田 賀弥"},{"name":"瀬山 真莉子"},{"name":"廣島 佑香"},{"name":"芽形 真奈"},{"name":"藤原 奈津美"},{"name":"工藤 保誠"},{"name":"尾崎 和美"}]},"description":{"en":"Objective: Porphyromonas gingivalis (Pg) is thought to be involved in the progression of Alzheimer's disease (AD). Whether Pg or its contents can reach the brain and directly affect neuropathology is, however, unknown. Here, we investigated whether outer membrane vesicles (OMVs) of Pg translocate to the brain and induce the pathogenic features of AD. Material and Methods: Pg OMVs were injected into the abdominal cavity of mice for 12 weeks. Pg OMV translocation to the brain was detected by immunohistochemistry using an anti-gingipain antibody. Tau protein and microglial activation in the mouse brain were examined by western blotting and immunohistochemistry. The effect of gingipains on inflammation was assessed by real-time polymerase chain reaction using human microglial HMC3 cells. Results: Gingipains were detected in the region around cerebral ventricles, choroid plexus, and ventricular ependymal cells in Pg OMV-administered mice. Tau and phosphorylated Tau protein increased and microglia were activated. Pg OMVs also increased the gene expression of proinflammatory cytokines in HMC3 cells in a gingipain-dependent manner. Conclusion: Pg OMVs, including gingipains, can reach the cerebral ventricle and induce neuroinflammation by activating microglia. Pg OMVs may provide a better understanding of the implications of periodontal diseases in neurodegenerative conditions such as AD.","ja":"Objective: Porphyromonas gingivalis (Pg) is thought to be involved in the progression of Alzheimer's disease (AD). Whether Pg or its contents can reach the brain and directly affect neuropathology is, however, unknown. Here, we investigated whether outer membrane vesicles (OMVs) of Pg translocate to the brain and induce the pathogenic features of AD. Material and Methods: Pg OMVs were injected into the abdominal cavity of mice for 12 weeks. Pg OMV translocation to the brain was detected by immunohistochemistry using an anti-gingipain antibody. Tau protein and microglial activation in the mouse brain were examined by western blotting and immunohistochemistry. The effect of gingipains on inflammation was assessed by real-time polymerase chain reaction using human microglial HMC3 cells. Results: Gingipains were detected in the region around cerebral ventricles, choroid plexus, and ventricular ependymal cells in Pg OMV-administered mice. Tau and phosphorylated Tau protein increased and microglia were activated. Pg OMVs also increased the gene expression of proinflammatory cytokines in HMC3 cells in a gingipain-dependent manner. Conclusion: Pg OMVs, including gingipains, can reach the cerebral ventricle and induce neuroinflammation by activating microglia. Pg OMVs may provide a better understanding of the implications of periodontal diseases in neurodegenerative conditions such as AD."},"publication_date":"2023-11","publication_name":{"en":"Oral Diseases","ja":"Oral Diseases"},"volume":"29","number":"8","starting_page":"3688","ending_page":"3697","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1111/odi.14413"],"issn":["1601-0825"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
{"insert":{"user_id":"R000041357","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://tokushima-u.repo.nii.ac.jp/records/2011911","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/36579753","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=394011","label":"url"}],"paper_title":{"en":"Lipocalin 2, synthesized using a cell-free protein synthesis system and encapsulated into liposomes, inhibits the adhesion of Porphyromonas gingivalis to human oral epithelial cells.","ja":"Lipocalin 2, synthesized using a cell-free protein synthesis system and encapsulated into liposomes, inhibits the adhesion of Porphyromonas gingivalis to human oral epithelial cells."},"authors":{"en":[{"name":"Kido Jun-ichi"},{"name":"Hiroshima Yuka"},{"name":"Kido Rie"},{"name":"Yoshida Kaya"},{"name":"Inagaki Yuji"},{"name":"Naruishi Koji"},{"name":"Kajimoto Kazuaki"},{"name":"Kataoka Masatoshi"},{"name":"Shinohara Yasuo"},{"name":"Yumoto Hiromichi"}],"ja":[{"name":"木戸 淳一"},{"name":"廣島 佑香"},{"name":"木戸 理恵"},{"name":"吉田 賀弥"},{"name":"稲垣 裕司"},{"name":"成石 浩司"},{"name":"Kajimoto Kazuaki"},{"name":"片岡 正俊"},{"name":"篠原 康雄"},{"name":"湯本 浩通"}]},"publication_date":"2023-04","publication_name":{"en":"Journal of Periodontal Research","ja":"Journal of Periodontal Research"},"volume":"58","number":"2","starting_page":"262","ending_page":"273","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1111/jre.13088"],"issn":["1600-0765"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
{"insert":{"user_id":"R000041357","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://tokushima-u.repo.nii.ac.jp/records/2011569","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/36834667","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=394204","label":"url"}],"paper_title":{"en":"Effects of Candidalysin Derived from Candida albicans on the Expression of Pro-Inflammatory Mediators in Human Gingival Fibroblasts","ja":"Effects of Candidalysin Derived from Candida albicans on the Expression of Pro-Inflammatory Mediators in Human Gingival Fibroblasts"},"authors":{"en":[{"name":"Nishikawa Yasufumi"},{"name":"Tomotake Yoritoki"},{"name":"Kawano Hiromichi"},{"name":"Naruishi Koji"},{"name":"Kido Jun-ichi"},{"name":"Hiroshima Yuka"},{"name":"Murakami Akikazu"},{"name":"Ichikawa Tetsuo"},{"name":"Yumoto Hiromichi"}],"ja":[{"name":"西川 泰史"},{"name":"友竹 偉則"},{"name":"川野 弘道"},{"name":"成石 浩司"},{"name":"木戸 淳一"},{"name":"廣島 佑香"},{"name":"村上 明一"},{"name":"市川 哲雄"},{"name":"湯本 浩通"}]},"publication_date":"2023-02-07","publication_name":{"en":"International Journal of Molecular Sciences","ja":"International Journal of Molecular Sciences"},"volume":"24","number":"4","starting_page":"3256","ending_page":"3256","languages":["eng"],"referee":true,"identifiers":{"doi":["10.3390/ijms24043256"],"issn":["1422-0067"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
{"insert":{"user_id":"R000041357","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://tokushima-u.repo.nii.ac.jp/records/2011910","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/36745267","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=394013","label":"url"}],"paper_title":{"en":"β-defensin 2 synthesized by a cell-free protein synthesis system and encapsulated in liposomes inhibits adhesion of Porphyromonas gingivalis to oral epithelial cells.","ja":"β-defensin 2 synthesized by a cell-free protein synthesis system and encapsulated in liposomes inhibits adhesion of Porphyromonas gingivalis to oral epithelial cells."},"authors":{"en":[{"name":"Hiroshima Yuka"},{"name":"Kido Jun-ichi"},{"name":"Kido Rie"},{"name":"Yoshida Kaya"},{"name":"Bandou Mika"},{"name":"Kajimoto Kazuaki"},{"name":"Yumoto Hiromichi"},{"name":"Shinohara Yasuo"}],"ja":[{"name":"廣島 佑香"},{"name":"木戸 淳一"},{"name":"木戸 理恵"},{"name":"吉田 賀弥"},{"name":"板東 美香"},{"name":"Kajimoto Kazuaki"},{"name":"湯本 浩通"},{"name":"篠原 康雄"}]},"publication_date":"2023-02-06","publication_name":{"en":"Odontology","ja":"Odontology"},"volume":"111","starting_page":"830","ending_page":"838","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1007/s10266-023-00789-x"],"issn":["1618-1255"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
{"insert":{"user_id":"R000041357","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://tokushima-u.repo.nii.ac.jp/records/2010419","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/36289904","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=392564","label":"url"}],"paper_title":{"en":"Porphyromonas gingivalis Outer Membrane Vesicles Stimulate Gingival Epithelial Cells to Induce Pro-Inflammatory Cytokines via the MAPK and STING Pathways","ja":"Porphyromonas gingivalis Outer Membrane Vesicles Stimulate Gingival Epithelial Cells to Induce Pro-Inflammatory Cytokines via the MAPK and STING Pathways"},"authors":{"en":[{"name":"Uemura Yuta"},{"name":"Hiroshima Yuka"},{"name":"Tada Ayano"},{"name":"Murakami Keiji"},{"name":"Yoshida Kaya"},{"name":"Inagaki Yuji"},{"name":"Kuwahara Tomomi"},{"name":"Murakami Akikazu"},{"name":"Fujii Hideki"},{"name":"Yumoto Hiromichi"}],"ja":[{"name":"植村 勇太"},{"name":"廣島 佑香"},{"name":"Tada Ayano"},{"name":"村上 圭史"},{"name":"吉田 賀弥"},{"name":"稲垣 裕司"},{"name":"桑原 知巳"},{"name":"村上 明一"},{"name":"藤猪 英樹"},{"name":"湯本 浩通"}]},"description":{"en":"() is a keystone pathogen associated with chronic periodontitis and produces outer membrane vesicles (OMVs) that contain lipopolysaccharide (LPS), gingipains, and pathogen-derived DNA and RNA. -OMVs are involved in the pathogenesis of periodontitis. -OMV-activated pathways that induce the production of the pro-inflammatory cytokines, interleukin (IL)-6, and IL-8 in the human gingival epithelial cell line, OBA-9, were investigated. The role of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB in levels of -OMV-induced pro-inflammatory cytokines was investigated using Western blot analysis and specific pathway inhibitors. -OMVs induced IL-6 and IL-8 production via the extracellular signal-regulated kinase (Erk) 1/2, c-Jun N-terminal kinase (JNK), p38 MAPK, and NF-κB signaling pathways in OBA-9 cells. In addition, the stimulator of interferon genes (STING), an essential innate immune signaling molecule, was triggered by a cytosolic pathogen DNA. -OMV-induced IL-6 and IL-8 mRNA expression and production were significantly suppressed by STING-specific small interfering RNA. Taken together, these results demonstrated that -OMV-activated Erk1/2, JNK, p38 MAPK, STING, and NF-κB signaling pathways resulting in increased IL-6 and IL-8 expression in human gingival epithelial cells. These results suggest that -OMVs may play important roles in periodontitis exacerbation by stimulating various pathways.","ja":"() is a keystone pathogen associated with chronic periodontitis and produces outer membrane vesicles (OMVs) that contain lipopolysaccharide (LPS), gingipains, and pathogen-derived DNA and RNA. -OMVs are involved in the pathogenesis of periodontitis. -OMV-activated pathways that induce the production of the pro-inflammatory cytokines, interleukin (IL)-6, and IL-8 in the human gingival epithelial cell line, OBA-9, were investigated. The role of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB in levels of -OMV-induced pro-inflammatory cytokines was investigated using Western blot analysis and specific pathway inhibitors. -OMVs induced IL-6 and IL-8 production via the extracellular signal-regulated kinase (Erk) 1/2, c-Jun N-terminal kinase (JNK), p38 MAPK, and NF-κB signaling pathways in OBA-9 cells. In addition, the stimulator of interferon genes (STING), an essential innate immune signaling molecule, was triggered by a cytosolic pathogen DNA. -OMV-induced IL-6 and IL-8 mRNA expression and production were significantly suppressed by STING-specific small interfering RNA. Taken together, these results demonstrated that -OMV-activated Erk1/2, JNK, p38 MAPK, STING, and NF-κB signaling pathways resulting in increased IL-6 and IL-8 expression in human gingival epithelial cells. These results suggest that -OMVs may play important roles in periodontitis exacerbation by stimulating various pathways."},"publication_date":"2022-10-20","publication_name":{"en":"Biomedicines","ja":"Biomedicines"},"volume":"10","number":"10","starting_page":"2643","ending_page":"2643","languages":["eng"],"referee":true,"identifiers":{"doi":["10.3390/biomedicines10102643"],"issn":["2227-9059"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}