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en: Large-scale isolation of mouse thymic epithelial cells

en: The thymus is compartmentalized into the cortex and the medulla. Cortical and medullary thymic epithelial cells (TECs) characterize T cell-producing and T cell-selecting functions of cortical and medullary microenvironments in the thymus. Enzymatic digestion of the thymus and flow cytometric isolation of TECs and their subpopulations are useful for molecular and cellular characterization of TECs. However, the cellularity of cTECs and mTECs isolated from mouse thymus is limited. In this chapter, we describe the method for isolation of a large number of TECs using enlarged mouse thymus, which enables biochemical and proteomic analysis of TEC subpopulations.


en: 免疫生物学(原書第9版) 監訳 笹月健彦，吉開泰信

ja: 免疫生物学(原書第9版) 監訳 笹月健彦，吉開泰信

en: Nankodo

ja: 南江堂


en: Thymocyte-mTEC cross talk for self-tolerance in T cells.

en: Elsevier

ja: Elsevier