Akari Watanabe, Takaku Satoru, Yokota Kenji, Hayashi Shunji, Naofumi Tamaki and Kokeguchi Susumu : A survey of Lasioderma serricorne (Fabricius) in Japanese Dental Clinics, Biocontrol Science, Vol.24, No.2, 117-121, 2019.
(要約)
This study was to survey the capturing rate in Japanese dental clinics of the Lasioderma serricorne (cigarette beetles) , and to evaluate the beetle's potential as a carrier for transmission of nosocomial pathogens. L. serricorne imagoes were captured in pheromone traps in 14 Japanese dental clinics in August and September 2012 and 2013, and their numbers recorded. Polymerase chain reaction (PCR) for the bacterial antibiotic-resistant genes mecA, vanA, vanB, blaIMP, and blaVIM was performed on the captured L. serricorne imagoes. Bacterial species in the captured specimens were identified by 16S rRNA PCR and sequencing analysis. The L. serricorne imagoes were captured from 10 dental clinics (71.4%) . We failed to detect the presence of nosocomial antibiotic-resistant pathogens in L. serricorne imagoes. The bacterial species detected most commonly in the imagoes was Wolbachia sp., an intracellular proteobacterium infecting certain insect species. Monitoring of insects including L. serricorne should be incorporated into regiment of the infection control.
Akari Watanabe, Naofumi Tamaki, Yokota Kenji, Miwa Matsuyama and Kokeguchi Susumu : Use of ATP bioluminescence to survey the spread of aerosol and splatter during dental treatments, The Journal of Hospital Infection, Vol.99, 303-305, 2018.
(要約)
Aerosol and splatter produced during dental treatments (ultrasonic scaling and professional mechanical tooth cleaning) are potential sources of infection. Contamination patterns on the mask, goggles, chest and gowned right arm of operators, and on the goggles of patients before and after dental treatments were investigated using ATP bioluminescence analysis. Contamination on every surface tested increased significantly after dental treatment. Maximum contamination was found on the goggles of patients. Aerosol and splatter produced during dental treatments therefore have the potential to spread infection to operators and patients. ATP bioluminescence is a useful tool for monitoring surface contamination.
Hidesuke Tada, Natsumi Fujiwara, Takaaki Tsunematsu, Yoshiko Tada, Rieko Arakaki, Naofumi Tamaki, Naozumi Ishimaru and Yasusei Kudo : Preventive effects of mouthguard use while sleeping on recurrent aphthous stomatitis: Preliminary interventional study., Clinical and Experimental Dental Research, Vol.3, No.5, 198-203, 2017.
(要約)
Recurrent aphthous stomatitis (RAS) is the most common inflammatory ulceration in the oral mucosa of otherwise healthy individuals and is often accompanied by severe pain. However, the etiology of RAS is not completely understood, and currently, no therapy can completely prevent RAS recurrence. In our clinical experience, we noticed that patients using a night guard, which is often used for bruxism treatment, did not develop RAS. Therefore, the aim of this study was to determine whether mouthguard use can suppress RAS development. The cohort of this interventional, prospective, single-center, and self-controlled study included 20 subjects who developed RAS at least once a month. The oral health of all the subjects was recorded for 60 days before and after intervention with a mouthguard. The average number of RAS incidences decreased from 5.5 to 1.0, the average days until healing decreased from 7.3 to 5.6, and the period with RAS decreased from 31.5 to 5.0 with mouthguard use. Mouthguard use may be beneficial for preventing RAS development.
Objective Oxidative stress is associated with the progression of chronic liver disease. Non-alcoholic fatty liver disease (NAFLD) is also an oxidative stress-related disease. However, the oxidative/anti-oxidative balance has not been fully characterized in NAFLD. The objective of the present study was to investigate the balance between oxidative stress and the anti-oxidative activity in NAFLD, including non-alcoholic steatohepatitis (NASH)-related hepatocellular carcinoma (HCC). Patients We recruited 69 patients with histologically proven NAFLD without HCC (NAFLD; n=58), and with NASH-related HCC (NASH-HCC; n=11). The 58 NAFLD patients included patients with non-alcoholic fatty liver (NAFL; n=14) and NASH (n=44). Methods The serum levels of reactive oxygen metabolites (ROM) and anti-oxidative markers (OXY) were determined and then used to calculate the oxidative index. The correlations among such factors as ROM, OXY, oxidative index, and clinical characteristics were investigated. Results In NAFLD, ROM positively correlated with the body mass index (BMI), hemoglobin A1c (HbA1c), C-reactive protein (CRP), and the histological grade or inflammatory scores, while only high HbA1c and CRP levels were significant factors that correlated with a higher ROM according to a multivariate analysis. OXY positively correlated with the platelet counts, albumin, and creatinine levels, while negatively correlating with age. However, it improved after treatment intervention. The oxidative index positively correlated with BMI, CRP, and HbA1c. The NASH-HCC patients exhibited a lower OXY than the NASH patients, probably due to the effects of aging. Conclusion Oxidative stress correlated with the levels of NASH activity markers, while the anti-oxidative function was preserved in younger patients as well as in patients with a well-preserved liver function. The NASH-HCC patients tended to be older and exhibited a diminished anti-oxidative function.
Akari Watanabe, Naofumi Tamaki, Kenji Yokota, Miwa Matsuyama and Susumu Kokeguchi : Monitoring of bacterial contamination of dental unit water lines using adenosine triphosphate bioluminescence, The Journal of Hospital Infection, Vol.94, No.4, 393-396, 2016.
(要約)
Bacterial contamination of dental unit waterlines (DUWLs) was evaluated using ATP bioluminescence analysis and a conventional culture method. Water samples (N=44) from DUWLs were investigated for heterotrophic bacteria by culture on R2A agar, which gave counts ranging from 1.4×10(3) to 2.7×10(5) cfu/mL. The ATP bioluminescence results for DUWL samples ranged from 6 to 1189 relative light units and could be obtained within 1min; these correlated well with the culture results (r=0.727-0.855). We conclude that the results of the ATP bioluminescence assay accurately reflect the results of conventional culture-based testing. This method is potentially useful for rapid and simple monitoring of DUWL bacterial contamination.
Akari Watanabe, Naofumi Tamaki, Miwa Matsuyama and Susumu Kokeguchi : Molecular analysis for bacterial contamination in dental unit water lines, The New Microbiologica, Vol.39, No.2, 143-145, 2016.
(要約)
Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.
Naofumi Tamaki, Rita Cristina Orihuela Campos, Makoto Fukui and Hiro-O Ito : Hydrogen-Rich Water Intake Accelerates Oral Palatal Wound Healing via Activation of the Nrf2/Antioxidant Defense Pathways in a Rat Model., Oxidative Medicine and Cellular Longevity, Vol.2016, 5679040, 2016.
(要約)
The wound healing process attempts to restore the integrity and function of the injured tissue. Additionally, proinflammatory cytokines, growth factors, and oxidative stress play important roles in wound healing. The aim of this study was to determine whether hydrogen-rich water intake induces the activation of the Nrf2/antioxidant defense pathway in rat palatal tissue, thereby reducing systemic oxidative stress and proinflammatory cytokine levels and promoting healing-associated genes. A circular excisional wound was created in the oral palatal region, and the wound healing process was observed. The rats were divided into two experimental groups in which either hydrogen-rich water or distilled water was consumed. In the drinking hydrogen-rich water, the palatal wound healing process was accelerated compared to that in the control group. As molecular hydrogen upregulated the Nrf2 pathway, systemic oxidative stresses were decreased by the activation of antioxidant activity. Furthermore, hydrogen-rich water intake reduced proinflammatory cytokine levels and promoted the expression of healing-associated factors in rat palatal tissue. In conclusion, hydrogen-rich water intake exhibited multiple beneficial effects through activation of the Nrf2/antioxidant defense pathway. The results of this study support the hypothesis that oral administration of hydrogen-rich water benefits the wound healing process by decreasing oxidative stress and inflammatory responses.
Masako Terao, Akinobu Takaki, Takayuki Maruyama, Hiroki Oe, Tetsuya Yasunaka, Naofumi Tamaki, Kazufumi Nakamura, Takaaki Tomofuji, Takahito Yagi, Hiroshi Sadamori, Yuzo Umeda, Susumu Shinoura, Ryuichi Yoshida, Kazuhiro Nouso, Daisuke Ekuni, Kazuko Koike, Fusao Ikeda, Hidenori Shiraha, Manabu Morita, Hiroshi Ito, Toshiyoshi Fujiwara and Kazuhide Yamamoto : Serum Oxidative/anti-oxidative Stress Balance Is Dysregulated in Potentially Pulmonary Hypertensive Patients with Liver Cirrhosis: A Case Control Study., Internal Medicine, Vol.54, No.22, 2815-2826, 2015.
(要約)
Objective Hepatopulmonary syndrome (HPS) is characterized by vascular dilatation and hyperdynamic circulation, while portopulmonary hypertension (POPH) is characterized by vasoconstriction with fibrous obliteration of the vascular bed. Vasoactive molecules such as nitric oxide (NO) are candidate factors for cirrhotic complications associated with these diseases. However, oxidative stress balance is not well characterized in HPS and POPH. The present objective is to investigate the oxidative stress and anti-oxidative stress balance and NO pathway balance in patients with potential HPS and POPH. Methods We recruited patients with decompensated cirrhosis (n=69) admitted to our hospital as liver transplantation candidates. Patients exhibiting partial pressure of oxygen lower than 80 mmHg and alveolar-arterial oxygen gradient (AaDO2) 15 mmHg were categorized as potentially having HPS (23 of 69 patients). Patients exhibiting a tricuspid regurgitation pressure gradient 25 mmHg were categorized as potentially having POPH (29 of 61 patients). Serum reactive oxygen metabolites were measured and anti-oxidative OXY-adsorbent test (OXY) were performed, and the balance of these tests was defined as the oxidative index. The correlation between these values and the clinical characteristics of the patients were assessed in a cross-sectional study. Results Potential HPS patients exhibited no correlation with oxidative stress markers. Potential POPH patients exhibited lower OXY (p=0.037) and higher oxidative index values (p=0.001). Additionally, the vascular NO synthase enzyme inhibiting protein, asymmetric dimethylarginine, was higher in potential POPH patients (p=0.049). The potential POPH patients exhibited elevated AaDO2, suggesting the presence of pulmonary shunting. Conclusion Potential POPH patients exhibited elevated oxidative stress with decreased anti-oxidative function accompanied by inhibited NO production. Anti-oxidants represent a candidate treatment for potential POPH patients.
Naofumi Tamaki, Fumihiko Yoshino, Makoto Fukui, Hideaki Hayashida, Ayaka Yoshida, Masayasu Kitamura, Takahiro Iwasaki, Reiko Furugen, Koji Kawasaki, Mio Nakazato, Takahiro Maeda, Susumu Kokeguchi, Tatsuo Yamamoto, Chang-Il Masaichi Lee, Hiro-O Ito and Toshiyuki Saito : Relationship among salivary antioxidant activity, cytokines and periodontitis: the Nagasaki Island study., Journal of Clinical Periodontology, Vol.42, No.8, 711-718, 2015.
(要約)
These findings suggest that the evaluation of salivary antioxidant activities, as assessed by electron spin resonance, are associated with periodontitis and various clinical variables in community-dwelling participants (ClinicalTrials. gov number NCT01742728). This article is protected by copyright. All rights reserved.
Rita Cristina Orihuela Campos, Naofumi Tamaki, Rie Mukai, Makoto Fukui, Kaname Miki, Junji Terao and Hiro-O Ito : Biological impacts of resveratrol, quercetin, and N-acetylcysteine on oxidative stress in human gingival fibroblasts., Journal of Clinical Biochemistry and Nutrition, Vol.56, No.3, 220-227, 2015.
(要約)
In periodontitis, production of reactive oxygen species (ROS) by neutrophils induces oxidative stress and deteriorates surrounding tissues. Antioxidants reduce damage caused by ROS and are used to treat diseases involving oxidative stress. This study summarizes the different effects of resveratrol, quercetin, and N-acetylcysteine (NAC) on human gingival fibroblasts (HGFs) under oxidative stress induced by hydrogen peroxide. Real-time cytotoxicity analyses reveals that resveratrol and quercetin enhanced cell proliferation even under oxidative stress. Of the antioxidants tested, resveratrol is the most effective at inhibiting ROS production. HGFs incubated with resveratrol and quercetin up-regulate the transcription of type I collagen gene after 3 h, but only resveratrol sustained this up-regulation for 24 h. A measurement of the oxygen consumption rate (OCR, mitochondrial respiration) shows that resveratrol generates the highest maximal respiratory capacity, followed by quercetin and NAC. Simultaneous measurement of OCR and the extracellular acidification rate (non-mitochondrial respiration) reveals that resveratrol and quercetin induce an increase in mitochondrial respiration when compared with untreated cells. NAC treatment consumes less oxygen and enhances more non-mitochondrial respiration. In conclusion, resveratrol is the most effective antioxidant in terms of real-time cytotoxicity analysis, reduction of ROS production, and enhancement of type I collagen synthesis and mitochondrial respiration in HGFs.
Naofumi Tamaki, Rita Cristina Orihuela Campos, Yuji Inagaki, Makoto Fukui, Toshihiko Nagata and Hiro-O Ito : Resveratrol improves oxidative stress and prevents the progression of periodontitis via the activation of the Sirt1/AMPK and the Nrf2/antioxidant defense pathways in a rat periodontitis model., Free Radical Biology and Medicine, Vol.75, 222-229, 2014.
(要約)
Oxidative stress is a key factor regulating the systemic pathophysiological effects associated with periodontitis. Resveratrol is a phytochemical with antioxidant and anti-inflammatory properties that can reduce oxidative stress and inflammation. We hypothesized that resveratrol may prevent the progression of periodontitis and reduce systemic oxidative stress through the activation of the Sirtuin1 (Sirt1)/AMP-activated protein kinase (AMPK) and the nuclear factor-E2 related factor 2 (Nrf2)/antioxidant defense pathways. Three groups of male Wistar rats (periodontitis treated with melinjo resveratrol, periodontitis without resveratrol, and control rats with no periodontitis or resveratrol treatment) were examined. A ligature was placed around the maxillary molars for 3 weeks to induce periodontitis, and the rats were then given drinking water with or without melinjo resveratrol. In rats with periodontitis, ligature placement induced alveolar bone resorption, quantified using three-dimensional images taken by micro-CT, and increased proinflammatory cytokine levels in gingival tissue. Melinjo resveratrol intake relieved alveolar bone resorption and activated the Sirt1/AMPK and the Nrf2/antioxidant defense pathways in inflamed gingival tissues. Further, melinjo resveratrol improved the systemic levels of 8-hydroxydeoxyguanosine, dityrosine, nitric oxide metabolism, nitrotyrosine, and proinflammatory cytokines. We concluded that oral administration of melinjo resveratrol may prevent the progression of ligature-induced periodontitis and improve systemic oxidative and nitrosative stress.
Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Mayu Yamane, Toshiki Yoneda, Yuya Kawabata, Kota Kataoka, Naofumi Tamaki and Manabu Morita : Longitudinal relationship between plasma reactive oxygen metabolites and periodontal condition in the maintenance phase of periodontal treatment., Disease Markers, Vol.2014, 489292, 2014.
(要約)
When plasma ROM level in periodontitis patients was low, increases in plasma ROM level were associated with those in CAL during the maintenance phase of treatment.
(キーワード)
Aged / Female / Humans / Longitudinal Studies / Male / Middle Aged / Oxidation-Reduction / Periodontal Attachment Loss / Periodontal Pocket / Periodontitis / Reactive Oxygen Species / Tooth
Naofumi Tamaki, H Hayashida, Makoto Fukui, M Kitamura, K Kawasaki, M Nakazato, T Maeda, T Saito and Hiro-O Ito : Oxidative stress and antibody levels to periodontal bacteria in adults: the Nagasaki Islands study., Oral Diseases, Vol.20, No.3, 49-56, 2014.
(要約)
OBJECTIVE: Periodontitis is a chronic inflammatory disease of the tissues supporting the teeth and is caused by subgingival plaque. Systemic increases in reactive oxygen species are involved in pathogenesis of periodontitis. This study addressed the relationship between levels of serum oxidative stress and antibodies against putative periodontopathic bacteria and their association with periodontal conditions, in a community-based study. SUBJECTS AND METHODS: Serum samples were measured for reactive oxygen metabolite (ROM) levels and anti-oxidant capacity. The serum levels of immunoglobulin G (IgG) antibodies to Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), and Eikenella corrodens (Ec) were determined by ELISA. RESULTS: The participants with greater clinical attachment loss had higher serum ROM levels and IgG antibody titers to Pg. Serum ROM levels were positively correlated with antibody titers to Pg, Pi, and Ec. When the participants with greater probing pocket depth and clinical attachment loss were used as the dependent variables, high ROM levels showed a statistically significant associations in multivariate logistic analyses; the adjusted odds ratios were 2.9 (95% confidence interval = 1.0-8.5) and 6.0 (95% confidence interval = 2.0-17.6), respectively. CONCLUSIONS: It was concluded that an increased oxidative stress may be detrimental to periodontitis in Japanese community-dwelling adults.
irie Koichiro, Takaaki Tomofuji, Ekuni Daisuke, Endo Yasumasa, Kasuyama Kenta, Tetsuji Azuma, Naofumi Tamaki, Yoneda Toshiki and Morita Manabu : Anti-ageing effects of dentifrices containing antioxidative, anti-inflammatory, and anti-bacterial agents (Tomarina) on gingival collagen degradation in rats, Archives of Oral Biology, Vol.59, No.1, 60-65, 2014.
(要約)
Previous studies have demonstrated the relationship between ageing and oxidative stress. In this study, we examined the effects of topical application of a dentifrice containing anti-oxidative, anti-inflammatory, and anti-bacterial agents (Tomarina®) to the gingival surface on gingival collagen degradation in rats. Fischer 344 male rats (4 or 8 months old) were divided into two groups: experimental group and control group. Tomarina® (the experimental group) or control dentifrice (the control group) was applied 5 days per week for 2 months. In the control group, gingival collagen density decreased with ageing. In the experimental group, the collagen density did not change with ageing, and was greater than that in the control group at 10 months of age (p < 0.0083). In addition, the control group showed an increase in serum oxidative stress with ageing. The experimental group also showed increased serum oxidative stress, but the value was lower than the control group at 10 months of age (p < 0.0083). Furthermore, low expressions of protein oxidative damage in the periodontal tissue were observed in the experimental group, compared to the control group at 6 months and 10 months. These findings indicate that Tomarina® might suppress the effects of ageing on gingival collagen degradation, by decreasing oxidative stress in the rat model.
AIM: Oxidative stress is associated with progression of chronic liver disease (CLD). This association is best established in chronic hepatitis C. However, the anti-oxidative state is not well characterized. The objective of the present study was to investigate the balance of oxidative and anti-oxidative stress in CLD patients. METHODS: We recruited a study population of 208 patients, including healthy volunteers (HV; n=15), patients with hepatitis B virus (HBV)-related CLD without or with hepatocellular carcinoma (HBV-non-HCC, n=25, and HBV-HCC, n=50, respectively), and patients with hepatitis C virus (HCV)-related CLD without or with HCC (HCV-non-HCC, n=49, and HCV-HCC, n=69, respectively). Serum levels of reactive oxygen metabolites (ROM) and anti-oxidative markers (OXY-adsorbent test; OXY) were determined, and the balance of these values was used as the oxidative index. Correlations among ROM, OXY, oxidative index and clinical characteristics were investigated. RESULTS: Patients with CLD exhibited elevated ROM and oxidative index compared to HV. Among patients with CLD, HCV positive status correlated with increased ROM. In CLD, HCV-HCC patients exhibited the highest ROM levels. Among HCV-related CLD patients, lower OXY correlated with HCC positive status, but was recovered by eradication of HCC. In HCV-HCC, lower OXY correlated with high PT-INR. CONCLUSION: HCV positive CLD patients displayed higher oxidative stress and HCV-HCC patients displayed lower anti-oxidative state. Anti-oxidative state depression was associated with liver reservoir-related data in HCV-HCC and could be reversed with HCC eradication.
Junko Kosaka, Hiroshi Morimatsu, Toru Takahashi, Hiroko Shimizu, Susumu Kawanishi, Emiko Omori, Yasumasa Endo, Naofumi Tamaki, Manabu Morita and Kiyoshi Morita : Effects of biliverdin administration on acute lung injury induced by hemorrhagic shock and resuscitation in rats., PLoS ONE, Vol.8, No.5, 2013.
(要約)
Hemorrhagic shock and resuscitation induces pulmonary inflammation that leads to acute lung injury. Biliverdin, a metabolite of heme catabolism, has been shown to have potent cytoprotective, anti-inflammatory, and anti-oxidant effects. This study aimed to examine the effects of intravenous biliverdin administration on lung injury induced by hemorrhagic shock and resuscitation in rats. Biliverdin or vehicle was administered to the rats 1 h before sham or hemorrhagic shock-inducing surgery. The sham-operated rats underwent all surgical procedures except bleeding. To induce hemorrhagic shock, rats were bled to achieve a mean arterial pressure of 30 mmHg that was maintained for 60 min, followed by resuscitation with shed blood. Histopathological changes in the lungs were evaluated by histopathological scoring analysis. Inflammatory gene expression was determined by Northern blot analysis, and oxidative DNA damage was assessed by measuring 8-hydroxy-2' deoxyguanosine levels in the lungs. Hemorrhagic shock and resuscitation resulted in prominent histopathological damage, including congestion, edema, cellular infiltration, and hemorrhage. Biliverdin administration prior to hemorrhagic shock and resuscitation significantly ameliorated these lung injuries as judged by histopathological improvement. After hemorrhagic shock and resuscitation, inflammatory gene expression of tumor necrosis factor- and inducible nitric oxide synthase were increased by 18- and 8-fold, respectively. Inflammatory gene expression significantly decreased when biliverdin was administered prior to hemorrhagic shock and resuscitation. Moreover, after hemorrhagic shock and resuscitation, lung 8-hydroxy-2' deoxyguanosine levels in mitochondrial DNA expressed in the pulmonary interstitium increased by 1.5-fold. Biliverdin administration prior to hemorrhagic shock and resuscitation decreased mitochondrial 8-hydroxy-2' deoxyguanosine levels to almost the same level as that in the control animals. We also confirmed that biliverdin administration after hemorrhagic shock and resuscitation had protective effects on lung injury. Our findings suggest that biliverdin has a protective role, at least in part, against hemorrhagic shock and resuscitation-induced lung injury through anti-inflammatory and anti-oxidant mechanisms.
Yoshikazu Fukumoto, Masumi Horibe, Yuji Inagaki, Keiji Oishi, Naofumi Tamaki, Hiro-O Ito and Toshihiko Nagata : Association of gingival recession and other factors with the presence of dentin hypersensitivity., Odontology, 2013.
(要約)
Dentin hypersensitivity (DH) may be present in association with gingival recession. The aim of this study was to determine quantitatively the association of gingival recession and other factors with the presence of DH. One hundred and four Japanese subjects with or without gingival recession were randomly selected. Intact canines and/or first premolars in both maxillary and mandibular quadrants were analyzed. Gingival recession was measured as a vertical length at the buccal site of the teeth. DH was recorded as an ordered categorical variable registering four increasing levels of pain after cold stimulation; from no discomfort to severe pain during and after stimulation (DH1, 2, 3, and 4). Association of DH with periodontal parameters and daily lifestyle was also investigated. Tooth-based analysis of 446 teeth from 104 subjects revealed that DH level was significantly higher in recessive teeth (1, 2, 3, and 4-8 mm) than in non-recessive teeth (0 mm). DH-positive rate in non-recessive teeth was only 18 % (DH1; 14 %, DH2; 3 %, and DH3; 1 %). Highest DH level was observed in teeth with severe recession (4-8 mm), showing DH0; 21 %, DH1; 33 %, DH2; 31 %, and DH3; 15 %. Recession-dependent increase in DH was observed, showing 18, 49, 52, 60, and 79 % DH-positive in teeth with 0, 1, 2, 3, and 4-8 mm recession, respectively. Plaque-free teeth showed a higher DH level than plaque-stained teeth, suggesting that good plaque control may be associated with the presence of DH. There were no significant differences in DH of teeth on the basis of smoking, probing depth, and bleeding on probing. Multiple logistic regression analysis revealed that gingival recession [odds ratio (OR) = 10.2, 95 % confidence interval (CI) = 5.5-18.9] and plaque deposition (OR = 0.3, 95 % CI = 0.2-0.5) were significant contributors to DH. Multilevel modeling analysis revealed that not only gingival recession and plaque deposition but also V-shaped cervical notch and tooth brushing frequency were associated with DH. These results demonstrate that the progression of gingival recession, plaque-free teeth, V-shaped cervical notch, and frequent brushing may be significant predictors of DH in canines and first premolars.
Daisuke Ekuni, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Shinsuke Mizutani, Azusa Kojima and Manabu Morita : Hydrogen-rich water prevents lipid deposition in the descending aorta in a rat periodontitis model., Archives of Oral Biology, Vol.57, No.12, 1615-1622, 2012.
(要約)
OBJECTIVE: Periodontitis has been causally linked to atherosclerosis, which is mediated by the oxidative stress. As hydrogen-rich water (HW) scavenges reactive oxygen species (ROS), we hypothesized that HW could prevent lipid deposition induced by periodontitis in the aorta. The aim of this study was to investigate the effects of HW on the initiation of atherosclerosis in a rat periodontitis model. DESIGN: Eighteen 8-wk-old male Wistar rats were divided into three groups of six rats; the periodontitis group, periodontitis+HW group and the no treatment (control) group. In the periodontitis and periodontitis+HW groups, periodontitis was induced using a ligature for 4 wk, while the periodontitis+HW group was given water containing 800-1000g/L hydrogen during the 4-wk experimental period. RESULTS: In the periodontitis group, lipid deposition in the descending aorta was observed. The periodontitis group also showed significant higher serum levels for ROS and oxidised low-density lipoprotein-cholesterol (ox-LDL) (1.7 and 1.4 times, respectively), and higher aortic expression levels of nitrotyrosine and hexanoyl-lysine (HEL) (7.9 and 16.0 times, respectively), as compared to the control group (p<0.05). In the periodontitis+HW group, lipid deposition was lower. Lower serum levels of ROS and ox-LDL (0.46 and 0.82 times, respectively) and lower aortic levels of nitrotyrosine and HEL (0.27 and 0.19 times, respectively) were observed in the periodontitis+HW group than in the periodontitis group (p<0.05). CONCLUSIONS: HW intake may prevent lipid deposition in the rat aorta induced by periodontitis by decreasing serum ox-LDL levels and aortic oxidative stress.
Daisuke Kawai, Akinobu Takaki, Atsuko Nakatsuka, Jun Wada, Naofumi Tamaki, Tetsuya Yasunaka, Kazuko Koike, Ryuichiro Tsuzaki, Kazuyuki Matsumoto, Yasuhiro Miyake, Hidenori Shiraha, Manabu Morita, Hirofumi Makino and Kazuhide Yamamoto : Hydrogen-rich water prevents progression of non-alcoholic steatohepatitis and accompanying hepatocarcinogenesis in mice., Hepatology, Vol.56, No.3, 912-921, 2012.
(要約)
Oxidative stress is a strong contributor to the progression from simple fatty liver to non-alcoholic steatohepatitis (NASH). Molecular hydrogen is an effective antioxidant that reduces cytotoxic reactive oxygen species (ROS). In this study, we investigated the effects of hydrogen-rich water (HW) and the drug pioglitazone (PGZ) on the progression of NASH in mouse models. A methionine-choline-deficient (MCD) diet mouse model was prepared. Mice were divided into three experimental groups and fed for 8 weeks as follows: 1) MCD diet + control water (CW group); 2) MCD diet + hydrogen-rich water (HW group); and 3) MCD diet mixed with pioglitazone (PGZ group). Plasma alanine aminotransferase levels, hepatic expression of TNF-, IL-6, fatty acid synthesis-related genes, oxidative stress biomarker 8-OHdG, and apoptosis marker TUNEL positive cells in the liver were decreased in the HW and PGZ groups. The HW group showed a smaller decrease in hepatic cholesterol; however, stronger anti-oxidative effects in serum and lower PPAR expression in liver were seen in comparison with the PGZ group. We then investigated the effects of hydrogen in the prevention of hepatocarcinogenesis in STAM® mice, known as the NASH-related hepatocarcinogenesis model. Eight-week-old male STAM® mice were divided into three experimental groups as follows: 1) control water (CW-STAM); 2) hydrogen-rich water (HW-STAM); and 3) pioglitazone (PGZ-STAM). After 8 weeks, hepatic tumors were evaluated. The number of tumors was significantly lower in the HW-STAM and PGZ-STAM groups than in the CW-STAM group. The maximum tumor size was smaller in the HW-STAM group than in the other groups. Conclusion: Consumption of hydrogen-rich water may be an effective treatment for NASH by reducing hepatic oxidative stress, apoptosis, inflammation and hepatocarcinogenesis. (HEPATOLOGY 2012.).
Kenta Kasuyama, Takaaki Tomofuji, Daisuke Ekuni, Naofumi Tamaki, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo and Manabu Morita : Hydrogen-rich water attenuates experimental periodontitis in a rat model., Journal of Clinical Periodontology, Vol.38, No.12, 1085-1090, 2011.
(要約)
The rats with periodontitis which were treated with pure water showed a time-dependent increase in serum ROS level. Compared with the rats without periodontitis, the periodontitis-induced rats which were given pure water also showed polymorphonuclear leucocyte infiltration and alveolar bone loss at 4 weeks. Hydrogen-rich water intake inhibited an increase in serum ROS level and lowered expression of 8-hydroxydeoxyguanosine and nitrotyrosine in the periodontal tissue at 4 weeks. Such conditions prevented polymorphonuclear leucocyte infiltration and osteoclast differentiation following periodontitis progression. Furthermore, inflammatory signalling pathways, such as mitogen-activated protein kinases, were less activated in periodontal lesions from hydrogen-rich water-treated rats as compared with pure water-treated rats.
(キーワード)
Animals / Disease Models, Animal / Free Radical Scavengers / Gingiva / Hydrogen / Male / Oxidative Stress / Periodontitis / Protons / Random Allocation / Rats / Rats, Wistar / Reactive Oxygen Species / Single-Blind Method / Water
Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takayuki Maruyama, Tatsuo Yamamoto, Tatsuo Watanabe and Manabu Morita : Relationships between periodontal inflammation, lipid peroxide and oxidative damage of multiple organs in rats., Biomedical Research, Vol.32, No.5, 343-349, 2011.
(要約)
Gingival response to periodontal inflammation generates excessive lipid peroxide and such a condition may augment systemic health through increased circulating lipid peroxide. The purpose of the present study was to investigate whether the generation of lipid peroxide in periodontal inflammation could induce tissue injury in the liver, heart, kidney and brain using a rat model. Twelve Wistar rats (8 week-old male) were divided into 2 groups: the periodontal inflammation group, receiving topical application of lipopolysaccharide and proteases to the gingival sulcus for 4 weeks, and the control group using instead pyrogen-free water. After blood samples were collected, specimens from the brain, heart, liver and kidney were resected to determine the concentration of 8-hydroxydeoxyguanosine (an indicator of oxidative DNA damage). Gingival and serum levels for hexanoyl-lysine were measured to evaluate lipid peroxide. Administration of lipopolysaccharide and proteases induced periodontal inflammation, with increasing gingival and serum levels of hexanoyl- lysine. The level of 8-hydroxydeoxyguanosine increased 2.27, 2.01, 1.49 and 1.40 times in mitochondrial DNA from the liver, heart, kidney and brain of rats with periodontal inflammation, respectively. The results reveal that excessive production of lipid peroxide following periodontal inflammation is involved in oxidative DNA damage of the brain, heart, liver and kidney.
(キーワード)
Animals / C-Reactive Protein / Lipid Peroxides / Liver / Male / Oxidation-Reduction / Periodontitis / Rats / Rats, Wistar
Naofumi Tamaki, Akinobu Takaki, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Daisuke Ekuni, Tetsuya Yasunaka, Kazuhide Yamamoto and Manabu Morita : Stage of hepatocellular carcinoma is associated with periodontitis., Journal of Clinical Periodontology, Vol.38, No.11, 1015-1020, 2011.
(要約)
The HCC + P and HCC + H groups had similar body mass index, habitual drinking and tobacco exposure data. The HCC + P group showed higher JIS scores than the HCC + H group (p = 0.027). Both the HCC + P and HCC + H groups had higher serum levels of ROM than controls (p < 0.001), while serum levels of ROM in the HCC + P group were a further 25.8% higher than those in the HCC + H group (p < 0.001).
M Furuta, T Tomofuji, D Ekuni, Naofumi Tamaki, T Yamamoto, T Azuma, K Irie, Y Endo, S Yamada and M Morita : Relationship between periodontal condition and arterial properties in an adult population in Japan., Oral Diseases, Vol.16, No.8, 781-787, 2010.
(要約)
Oral Diseases (2010) 16, 781-787 Objective: This study addressed the relationship between periodontal condition and second derivative of the finger photoplethysmogram (SDPTG) in Japanese adults. Subject and methods: The Community Periodontal Index (CPI) and SDPTG were recorded in 415 subjects (mean age: 44.0 years). For assessing SDPTG, we mainly focused on the ratio of the absolute value of the height of the early negative 'b' wave and ratio of the late re-decreasing 'd' wave to the height of the initial positive 'a' wave, namely the b/a and d/a ratios. Results: The CPI score was positively correlated with the b/a ratio (P < 0.001), and negatively correlated with the d/a ratio (P < 0.001). Logistic regression analysis showed that subjects with CPI scores ≥ 3 were more likely to have a higher level (male > -0.69, female > -0.64) of b/a ratio (Odds ratio = 1.7, P = 0.026) and lower level (male ≤ -0.29, female ≤ -0.32) of d/a ratio (Odds ratio = 2.2, P =0.001) than those with CPI scores 0-2, after adjusting for age, gender, smoking status, pulse rate and presence of hypertension. Conclusion: There was a statistical association between the CPI scores and SDPTG indices in Japanese adults.
(キーワード)
Adult / Age Factors / Arteries / Blood Volume / Cross-Sectional Studies / Dental Calculus / Female / Fingers / Gingival Hemorrhage / Hemodynamics / Humans / Hypertension / Japan / Male / Middle Aged / Periodontal Index / Periodontal Pocket / Photoplethysmography / Pulsatile Flow / Pulse / Sex Factors / Smoking / Young Adult
Takayuki Maruyama, Takaaki Tomofuji, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Daisuke Ekuni, Naofumi Tamaki, Tatsuo Yamamoto and Manabu Morita : Supplementation of green tea catechins in dentifrices suppresses gingival oxidative stress and periodontal inflammation., Archives of Oral Biology, Vol.56, No.1, 48-53, 2010.
(要約)
adding green tea catechins to a dentifrice may contribute to prevention of periodontal inflammation by decreasing gingival oxidative stress and expression of pro-inflammatory cytokines.
Periodontitis induces excessive production of reactive oxygen species in periodontal lesions. This may impair circulating pro-oxidant/anti-oxidant balance and induce the oxidation of low-density lipoprotein (LDL) in blood. The purpose of this study was to monitor circulating oxidized LDL and oxidative stress in subjects with chronic periodontitis following non-surgical periodontal treatment. Plasma levels of oxidized LDL and oxidative stress in 22 otherwise healthy non-smokers with chronic periodontitis (mean age 44.0 years) were measured at baseline and at 1 and 2 months after non-surgical periodontal treatment. At baseline, chronic periodontitis patients had higher plasma levels of oxidized LDL and oxidative stress than healthy subjects (p < 0.001). Periodontal treatment was associated with a significant reduction in plasma levels of oxidized LDL (oxLDL)(p < 0.001) and oxidative stress (p < 0.001). At 2 months after periodontal treatment, the degree of change in the oxLDL was positively correlated with that in the oxidative stress (r = 0.593, p = 0.004). These observations indicate that periodontitis patients showed higher levels of circulating oxLDL and oxidative stress than healthy subjects. In addition, improved oral hygiene and non-surgical periodontal treatment were effective in decreasing oxLDL, which was positively associated with a reduction in circulating oxidative stress.
Yasumasa Endo, Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Tatsuo Yamamoto and Manabu Morita : Experimental periodontitis induces gene expression of proinflammatory cytokines in liver and white adipose tissues in obesity., Journal of Periodontology, Vol.81, No.4, 520-526, 2010.
(要約)
Systemic low-grade inflammation after experimental periodontitis was associated with increased gene expression for hepatic levels of TNF-alpha and CRP and adipose tissue levels of IL-6 and CRP in the obese-rat model.
(キーワード)
Adipose Tissue, White / Analysis of Variance / Animals / C-Reactive Protein / Cytokines / Gene Expression / Inflammation Mediators / Interleukin-6 / Ligation / Liver / Male / Obesity / Periodontitis / RNA, Messenger / Random Allocation / Rats / Rats, Zucker / Tumor Necrosis Factor-alpha / Up-Regulation
Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Kenta Kasuyama, Michihiro Umakoshi, Tetsuji Azuma, Naofumi Tamaki, Toshihiro Sanbe, Yasumasa Endo, Tatsuo Yamamoto, Takashi Nishida and Manabu Morita : Effects of periodontitis on aortic insulin resistance in an obese rat model., Laboratory Investigation; a Journal of Technical Methods and Pathology, Vol.90, No.3, 348-359, 2010.
(要約)
The combination of obesity and its associated risk factors, such as insulin resistance and inflammation, results in the development of atherosclerosis. However, the effects of periodontitis on atherosclerosis in an obese body remain unclear. The aim of the study was to investigate the effects of ligature-induced periodontitis in Zucker fatty rats on initiation of atherosclerosis by evaluating aortic insulin resistance. Zucker fatty rats (n=24) were divided into two groups. In the periodontitis group, periodontitis was ligature-induced for 4 weeks, whereas the control group was left unligated. After the 4-week experimental period, descending aorta was used for measuring the levels of lipid deposits, immunohistochemical analysis, and evaluation of gene expression. Levels of serum C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-alpha), and insulin were also measured. Rats in the periodontitis group had significantly enhanced lipid deposits in the aorta, but not in the control group. Expression of suppressor of cytokine signaling 3, vascular cell adhesion molecule 1, reactive oxygen species, nitrotyrosine, and endothelin-1 in the periodontitis group was more intense than that in the control group. Significantly decreased levels of phosphatidylinositol 3-kinase (Pi3k) catalytic beta-polypeptide (Pi3kcb), Pi3kp85, and insulin receptor substrate 1 and 2 were observed in the periodontitis group. Levels of serum CRP and TNF-alpha were significantly increased in the periodontitis group. Under insulin-stimulated conditions, aorta in the periodontitis group altered the Akt phosphorylation. Periodontitis in obesity induced the initial stage of atherosclerosis and disturbed aortic insulin signaling.
Daisuke Ekuni, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takaaki Tomofuji and Manabu Morita : Imbalance of oxidative/anti-oxidative status induced by periodontitis is involved in apoptosis of rat submandibular glands., Archives of Oral Biology, Vol.55, No.2, 170-176, 2009.
(要約)
Imbalance of circulating oxidative/anti-oxidative status may be involved in vacuolisation and apoptosis of submandibular glands in the rat periodontitis model.
Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Yasumasa Endo, Naofumi Tamaki, Toshihiro Sanbe, Jun Murakami, Tatsuo Yamamoto and Manabu Morita : Preventive effects of a cocoa-enriched diet on gingival oxidative stress in experimental periodontitis., Journal of Periodontology, Vol.80, No.11, 1799-1808, 2009.
(要約)
Consuming a cocoa-enriched diet could diminish periodontitis-induced oxidative stress, which, in turn, might suppress the progression of periodontitis.
Takaaki Tomofuji, Tatsuo Yamamoto, Naofumi Tamaki, Daisuke Ekuni, Tetsuji Azuma, Toshihiro Sanbe, Koichiro Irie, Kenta Kasuyama, Michihiro Umakoshi, Jun Murakami, Susumu Kokeguchi and Manabu Morita : Effects of obesity on gingival oxidative stress in a rat model., Journal of Periodontology, Vol.80, No.8, 1324-1329, 2009.
(要約)
Obesity induced gingival oxidative stress with increasing serum reactive oxygen metabolites in rats. In the periodontal lesion, gene expressions related to a capacity for xenobiotic detoxification were downregulated in the obese model.
(キーワード)
Alveolar Bone Loss / Animals / Body Weight / Cytochrome P-450 Enzyme System / Deoxyguanosine / Disease Models, Animal / Down-Regulation / Gingiva / Gingivitis / Glutathione / Leukocyte Count / Male / Neutrophils / Obesity / Oxidation-Reduction / Oxidative Stress / Periodontitis / Rats / Rats, Zucker / Reactive Oxygen Species
T Yamamoto, T Tomofuji, Naofumi Tamaki, D Ekuni, T Azuma and T Sanbe : Effects of topical application of lipopolysaccharide and proteases on hepatic injury induced by high-cholesterol diet in rats., Journal of Periodontal Research, Vol.45, No.1, 129-135, 2009.
(要約)
In a rat model, application of lipopolysaccharide and proteases to the gingival sulcus augmented the effect of a high-cholesterol diet on steatosis, inflammation and oxidative damage in the liver.
R Yamanaka, R Akther, M Furuta, R Koyama, T Tomofuji, D Ekuni, Naofumi Tamaki, T Azuma, T Yamamoto and E Kishimoto : Relation of dietary preference to bite force and occlusal contact area in Japanese children., Journal of Oral Rehabilitation, Vol.36, No.8, 584-591, 2009.
(要約)
The purpose of this study was to examine the relationship of dietary preference to bite force and occlusal contact area in Japanese elementary school children. A total of 348 children, aged 7-12 years, from two public elementary schools located in Okayama Prefecture, Japan, participated in the study. Clinical examination included decayed, missing and filled teeth (dmft and DMFT), and total numbers of deciduous and permanent teeth. Bite force and occlusal contact area were measured using a pressure-detecting sheet. Dietary preference was assessed using a questionnaire in which the answers were given in like/dislike form. Mann-Whitney U-test and multiple logistic regression analysis were applied to analyse the data. In multiple logistic regression analysis after adjustment for age, gender and total number of teeth present, children who liked cabbage and celery showed significantly higher bite force (P = 0.05 and P < 0.01, respectively) than those who disliked these. Children who liked cabbage and celery also showed higher occlusal contact area (P < 0.05 and P < 0.01, respectively) than those who disliked these. The Japanese elementary school children who liked hard foods such as cabbage and celery showed higher bite force and higher occlusal contact area than those who disliked these foods. A positive attitude towards harder food items might contribute to healthy development of the masticatory apparatus.
(キーワード)
Asian Continental Ancestry Group / Bite Force / Child / Dental Occlusion / Female / Food Preferences / Humans / Japan / Male / Mastication / Reproducibility of Results / Statistics, Nonparametric
Naofumi Tamaki, Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Tatsuo Yamamoto and Manabu Morita : Short-term effects of non-surgical periodontal treatment on plasma level of reactive oxygen metabolites in patients with chronic periodontitis., Journal of Periodontology, Vol.80, No.6, 901-906, 2009.
(要約)
In patients with chronic periodontitis, non-surgical periodontal treatment was effective at improving clinical parameters and reducing plasma ROMs. The improvement in chronic periodontitis by non-surgical periodontal treatment might offer clinical benefits by decreasing blood ROS.
Daisuke Ekuni, Takaaki Tomofuji, Toshihiro Sanbe, Koichiro Irie, Tetsuji Azuma, Takayuki Maruyama, Naofumi Tamaki, Jun Murakami, Susumu Kokeguchi and Tatsuo Yamamoto : Vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress., Archives of Oral Biology, Vol.54, No.5, 495-502, 2009.
(要約)
These results suggest that vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress.
D Ekuni, T Tomofuji, T Sanbe, K Irie, T Azuma, T Maruyama, Naofumi Tamaki, J Murakami, S Kokeguchi and T Yamamoto : Periodontitis-induced lipid peroxidation in rat descending aorta is involved in the initiation of atherosclerosis., Journal of Periodontal Research, Vol.44, No.4, 434-442, 2009.
(要約)
In a ligature-induced periodontitis rat model, increased lipid peroxidation was found in serum and aorta as well as in periodontal tissue. Atherosclerosis-related gene expression and histological changes were also stimulated. Periodontitis-induced lipid peroxidation in the aorta may be involved in the early stage of atherosclerosis.
(キーワード)
Animals / Aorta, Thoracic / Aortic Diseases / Atherosclerosis / Disease Models, Animal / Down-Regulation / Fibroblasts / Hydrogen Peroxide / Lipid Peroxidation / Lipids / Lysine / Male / Microarray Analysis / Molar / Nitric Oxide Synthase Type II / Nitric Oxide Synthase Type III / Oxidative Stress / Periodontal Ligament / Periodontitis / Random Allocation / Rats / Rats, Wistar / Reactive Oxygen Species / Superoxide Dismutase
Takaaki Tomofuji, Daisuke Ekuni, Toshihiro Sanbe, Tetsuji Azuma, Naofumi Tamaki, Takayuki Maruyama, Tatsuo Yamamoto, Tatsuo Watanabe, Mutsumi Miyauchi and Takashi Takata : Effects of improvement in periodontal inflammation by toothbrushing on serum lipopolysaccharide concentration and liver injury in rats., Acta Odontologica Scandinavica, Vol.67, No.4, 200-205, 2009.
(要約)
Toothbrushing promoted healing of periodontal lesions, decreased serum lipopolysaccharide concentration and suppressed liver injury in a rat periodontitis model.
Toshihiro Sanbe, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Naofumi Tamaki, Tatsuo Yamamoto and Manabu Morita : Vitamin C intake inhibits serum lipid peroxidation and osteoclast differentiation on alveolar bone in rats fed on a high-cholesterol diet., Archives of Oral Biology, Vol.54, No.3, 235-240, 2008.
(要約)
Vitamin C intake could suppress osteoclast differentiation, including RANKL and NF-kappaB expression on the alveolar bone surface, by decreasing serum lipid peroxidation in rats fed a high-cholesterol diet.
Naofumi Tamaki, Takaaki Tomofuji, Takayuki Maruyama, Daisuke Ekuni, Reiko Yamanaka, Noriko Takeuchi and Tatsuo Yamamoto : Relationship between periodontal condition and plasma reactive oxygen metabolites in patients in the maintenance phase of periodontal treatment., Journal of Periodontology, Vol.79, No.11, 2136-2142, 2008.
(要約)
A positive association was found between plasma oxidative status and CAL in patients in the maintenance phase of periodontal therapy. A systemic increase in oxidative stress may influence the rate of progression of periodontal disease.
(キーワード)
Adult / Aged / Aged, 80 and over / Antioxidants / Cross-Sectional Studies / Dental Care / Female / Humans / Male / Middle Aged / Oral Hygiene / Oxidative Stress / Periodontal Attachment Loss / Periodontal Diseases / Periodontal Index / Periodontium / Reactive Oxygen Species / Young Adult
Takaaki Tomofuji, Daisuke Ekuni, Toshihiro Sanbe, Koichiro Irie, Tetsuji Azuma, Takayuki Maruyama, Naofumi Tamaki, Jun Murakami, Susumu Kokeguchi and Tatsuo Yamamoto : Effects of vitamin C intake on gingival oxidative stress in rat periodontitis., Free Radical Biology and Medicine, Vol.46, No.2, 163-168, 2008.
(要約)
Increased levels of oxidative stress due to excessive production of reactive oxygen species are involved in the pathogenesis of periodontitis. Studies suggest a negative association between plasma vitamin C level and the severity of periodontitis. We hypothesized that increases in plasma vitamin C levels after vitamin C intake might clinically reduce gingival oxidative stress in a rat periodontitis model. A ligature was placed around rat mandibular molars for 4 weeks to induce periodontitis, and the rats were then given drinking water with or without 1 g/L vitamin C for 2 weeks after the ligature was removed. The periodontitis-induced rats showed a 149% increase in 8-hydroxydeoxyguanosine level and a 40% decrease in reduced:oxidized glutathione ratio in gingival tissue. Vitamin C intake induced a 175% increase in plasma vitamin C level, resulting in an improvement in the gingival 8-hydroxydeoxyguanosine level (decreased) and in the reduced:oxidized glutathione ratio (increased). Furthermore, in ligature-induced periodontitis lesions, gene expression encoding inflammation, including interleukin-1 alpha and interleukin-1 beta, was more than twofold down-regulated by vitamin C intake. The results suggest that systemic administration of vitamin C could be clinically beneficial in improving periodontitis-induced oxidative stress by down-regulating inflammatory gene expression.
Takaaki Tomofuji, Toshihiro Sanbe, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Takayuki Maruyama, Naofumi Tamaki and Tatsuo Yamamoto : Oxidative damage of rat liver induced by ligature-induced periodontitis and chronic ethanol consumption., Archives of Oral Biology, Vol.53, No.12, 1113-1118, 2008.
(要約)
In the rat model, ligature-induced periodontitis increased plasma lipid peroxide, decreased the hepatic GSH/GSSG ratio and augmented ethanol-induced lipid peroxidation in the liver.
K Irie, T Tomofuji, Naofumi Tamaki, T Sanbe, D Ekuni, T Azuma, T Maruyama and T Yamamoto : Effects of ethanol consumption on periodontal inflammation in rats., Journal of Dental Research, Vol.87, No.5, 456-460, 2008.
(要約)
Studies suggest a correlation between ethanol consumption and periodontal disease. We hypothesized that elevated levels of blood reactive oxygen species following ethanol consumption may increase inflammation in periodontal tissue. Rats were divided into 4 groups (6-7 rats/group). Two groups were fed an ethanol-containing liquid diet, and 2 groups were fed a pair-fed control diet. In one of each dietary group, periodontitis was ligature-induced, while the other group was left unligated. Chronic ethanol feeding alone decreased the ratio of reduced/oxidized glutathione and increased 8-hydroxydeoxy-guanosine and tumor necrosis factor (TNF)-alpha levels in the gingiva. Blood hydroperoxides were also increased. In ligature-induced periodontitis lesions, ethanol feeding enhanced polymorpho-nuclear leukocyte infiltration and TNF-alpha expression. The results suggest that chronic alcohol consumption increased periodontal inflammation, oxidative damage, and TNF-alpha production and had an additive effect on polymorphonuclear leukocyte infiltration and gingival oxidative damage, increasing the severity of periodontal inflammation in the ligature model.
Daisuke Ekuni, Takaaki Tomofuji, Naofumi Tamaki, Toshihiro Sanbe, Tetsuji Azuma, Reiko Yamanaka, Tatsuo Yamamoto and Tatsuo Watanabe : Mechanical stimulation of gingiva reduces plasma 8-OHdG level in rat periodontitis., Archives of Oral Biology, Vol.53, No.4, 324-329, 2007.
(要約)
Mechanical stimulation of periodontally involved gingiva reduced 8-hydroxydeoxyguanosine in plasma and may contribute to a reduction in circulating oxidative stress associated molecules.
Toshihiro Sanbe, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Naofumi Tamaki and Tatsuo Yamamoto : Oral administration of vitamin C prevents alveolar bone resorption induced by high dietary cholesterol in rats., Journal of Periodontology, Vol.78, No.11, 2165-2170, 2007.
(要約)
In the rat model, vitamin C suppressed alveolar bone resorption, induced by high dietary cholesterol, by decreasing the oxidative damage of periodontal tissue.
(キーワード)
Alveolar Process / Animals / Antioxidants / Ascorbic Acid / Bone Density / Bone Resorption / Cholesterol / Diet / Hyperlipidemias / Male / Osteoclasts / Rats / Rats, Wistar
Midori Tsuneishi, Tatsuo Yamamoto, Susumu Kokeguchi, Naofumi Tamaki, Kazuhiro Fukui and Tatsuo Watanabe : Composition of the bacterial flora in tonsilloliths., Microbes and Infection, Vol.8, No.9-10, 2384-2389, 2006.
(要約)
Tonsilloliths are a potential cause of oral malodor. In this study, microbial profiles and composition of tonsilloliths were determined using culture-independent molecular methods and scanning electron microscopy. 16S ribosomal RNA bacterial genes (16S rDNAs) isolated from tonsilloliths of 6 individuals were amplified by PCR and cloned into Escherichia coli. Partial 16S rDNA sequences of approximately 600 bases of cloned inserts were used to determine species identity by comparison with sequences of known species. Characteristics of bacteria on the surface and inside the tonsillolith were analyzed using scanning electron microscopy. Anaerobic bacteria detected in tonsilloliths belonged to the genera Eubacterium, Fusobacterium, Megasphaera, Porphyromonas, Prevotella, Selenomonas and Tannerella, all of which appear to be associated with production of volatile sulfur compounds. Electron microscopy revealed cocci and rods on the surface and rods predominating inside the tonsilloliths. These results support the tonsillolith as an origin of oral malodor.
Takaaki Tomofuji, Tetsuji Azuma, Hiroki Kusano, Toshihiro Sanbe, Daisuke Ekuni, Naofumi Tamaki, Tatsuo Yamamoto and Tatsuo Watanabe : Oxidative damage of periodontal tissue in the rat periodontitis model: effects of a high-cholesterol diet., FEBS Letters, Vol.580, No.15, 3601-3604, 2006.
(要約)
Studies suggest an association between consumption of a high-cholesterol diet and periodontitis. We addressed the mechanism by which high dietary cholesterol could be detrimental to periodontal health in a rat model. Feeding a high-cholesterol diet augmented the effects of bacterial pathogens and their products (e.g., lipopolysaccharide and proteases) on production of pro-inflammatory cytokines in fibroblasts. High dietary cholesterol also increased mitochondrial 8-hydroxydeoxyguanosine in the periodontal tissues. These results suggest that excessive tissue oxidative damage induced by high dietary cholesterol could potentiate pro-inflammatory cytokine production by fibroblasts stimulated with bacterial pathogens.
Midori Tsuneishi, Tatsuo Yamamoto, Reiko Yamanaka, Naofumi Tamaki, Tomonori Sakamoto, Kohzoh Tsuji and Tatsuo Watanabe : Radiographic evaluation of periapical status and prevalence of endodontic treatment in an adult Japanese population., Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, Vol.100, No.5, 631-635, 2005.
(要約)
The prevalence of root-filled teeth appears higher in this Japanese population than in Europe or America; however, the ratio of teeth with an apical radiolucency to root-filled teeth was within the range of that reported for other countries. Overfilled teeth and mandibular incisors are most likely to exhibit apical radiolucencies.
(キーワード)
Adult / Age Distribution / Aged / Aged, 80 and over / Chi-Square Distribution / Extravasation of Diagnostic and Therapeutic Materials / Female / Humans / Japan / Logistic Models / Male / Middle Aged / Observer Variation / Peer Review, Health Care / Periapical Periodontitis / Periodontal Index / Prevalence / Quality of Health Care / Root Canal Therapy
K Ohishi, T Yamamoto, T Tomofuji, Naofumi Tamaki and T Watanabe : Isolation and characterization of aminopeptidase from Capnocytophaga granulosa ATCC 51502., Oral Microbiology and Immunology, Vol.20, No.2, 67-72, 2005.
(要約)
There is evidence that enzymes from the genus Capnocytophaga play a role in dental calculus formation. Although most of the species in the genus produce aminopeptidases, there is a paucity of data on the purification and characterization of the enzyme, except in the case of Capnocytophaga gingivalis. The aim of this study was to purify aminopeptidase from culture supernatant of Capnocytophaga granulosa ATCC 51502, a new species of the genus. Purification was performed using ammonium sulfate fractionation and two chromatographic steps. The aminopeptidase was purified 158,433-fold with a yield of 12.0%. The enzyme appeared to be a trimer with a molecular mass of 270 kDa. The optimal pH of the aminopeptidase was 6.5 and its activity was completely inhibited by incubation at 50 degrees C for 10 min. The enzyme showed maximum specificity for basic amino acids (Arg and Lys) and also hydrolyzed noncharged amino acids (Met, Leu and Ala). Ca(2+), Zn(2+) and Fe(3+) activated the enzyme, while EDTA, Ag(+), Hg(+) and Cu(2+) inhibited it. These results suggest that aminopeptidase of C. granulosa is different from that of C. gingivalis but similar to aminopeptidase B.
(キーワード)
Amino Acids / Aminopeptidases / Capnocytophaga / Chemical Fractionation / Chromatography, Ion Exchange / Dental Calculus / Electrophoresis, Polyacrylamide Gel / Isoelectric Point / Molecular Weight / Protease Inhibitors / Substrate Specificity
Naofumi Tamaki, T Tada, M Morita and T Watanabe : Comparison of inhibitory activity on calcium phosphate precipitation by acidic proline-rich proteins, statherin, and histatin-1., Calcified Tissue International, Vol.71, No.1, 59-62, 2002.
(要約)
This study quantitatively compares the inhibition of calcium phosphate (CaP) precipitation by the salivary acidic proline-rich proteins (PRPs) statherin and histatin-1. Saliva and CaCl2 in 125 mM imidazole buffer (pH 7.0) were incubated with potassium phosphate and a hydroxyapatite (HAP) suspension, for 30 min at 25 degrees C, then filtered through nitrocellulose. The calcium (Ca) concentration in the filtrate was measured by atomic absorption spectrophotometry, then deducted from that in the initial solution to determine the amount of CaP precipitation after 30 min. The values of the inhibitory activities on CaP precipitation relative to crude parotid saliva were 4.7, 4.9, 6.9, and 65.8 for histatin-1, large PRPs, small PRPs, and statherin, respectively.
玉木 直文 : Periodontitis and Antioxidants, Journal of Oral Health and Biosciences, Vol.27, No.2, 89-96, 2015年2月.
(要約)
Reactive oxygen species (ROS), which include super oxide (O2-・), hydroxyl radical (HO・), singlet oxygen (1O2) and hydrogen peroxide (H2O2), are products of normal cellular metabolism. ROS are well recognized for playing a dual role as both deleterious and beneficial effects to living systems.The harmful effects occur in biological systems when there is a overproduction of ROS on one side and a lack of enzymatic or non-enzymatic antioxidants on the other side. Furthermore, oxidative stress results from the metabolic reactions which use oxygen and represents a disturbance in the equilibrium status of antioxidants in living organisms. The excess of ROS can damage proteins, cellular lipids, and DNA inhibiting their normal function. Therefore, oxidative stress has been implicated in a number of human diseases as well as periodontitis. Recently, clinical studies have noted that the patients of periodontitis have elevated blood levels of oxidative stress compared to periodontally healthy subjects in the cross sectional design. Additionally, the increased oxidative stress is significantly associated with the progression of periodontitis. In longitudinal study, periodontal treatment decreases oxidized low density lipoprotein levels and total oxidative status in the blood of chronic periodontitis patients.In recent years, many compounds and plant extracts have considerable antioxidant activity, and applied to animal experimental periodontitis models. Investigations provided their possibility for preventive effects on periodontitis. For example, polyphenol including flavonoid revealed both antioxidant and anti-inflammation effect, suppressing the progression of periodontitis by decreasing gingival oxidative stress. In particular, rats were given resveratrol as drinking water and experimental periodontitis was induced in our study. As a result, resveratrol intake relieved alveolar bone resorption and activated the Sirtuin1 / AMP-activated protein kinase and the nuclear factor E2-related factor 2 / antioxidant defense pathways in inflamed gingival tissues. Moreover, resveratrol improved the systemic levels of 8-hydroxy-2'-deoxyguanosine, dityrosine, nitric oxide metabolism, nitrotyrosine, and proinflammatory cytokines. We concluded that oral administration of antioxidants could prevent the progression of experimental periodontitis and improve systemic oxidative stress.
Akari Watanabe, Kenji Yokota, Naofumi Tamaki and Susumu Kokeguchi : Microbial Contamination and Growth in Feeding Cups, 4th Meeting of the International Association for Dental Research Asia Pacific Region 2019,Australia, 100, Nov. 2019.
2.
Akari Watanabe, Yokota Kenji, Naofumi Tamaki and Kokeguchi Susumu : Effects of Oral Cleaning and Mouthwash in the Hospitalized Elderly, 96th General Session & Exhibition of the IADR IADR Pan European Regional Congress, London, Jul. 2018.
3.
Akari Watanabe, Naofumi Tamaki, Kenji Yokota, Susumu Kokeguchi and Miwa Matsuyama : Survey of Dissemination of Aerosol and Splatter during Dental Treatments, The4th ASEAN plus and TOKUSHIMA Joint International Conference on Challenging and Innovation in Oral Sciences, 28, Bali, Dec. 2017.
4.
Akari Watanabe, Naofumi Tamaki, Kenji Yokota, Miwa Matsuyama and Susumu Kokeguchi : Survey of Methicillin-Resistant Staphylococci From Hospital Environment in Japan, International Union of Microbiological Societies(IUMS2017), singapore, Jul. 2017.
5.
Akari Watanabe, Naofumi Tamaki and Susumu Kokeguchi : Bacterial-Contamination Monitoring of Dental Unit Water Lines Using ATP-Bioluminescence Analysis, 94th General Session & Exhibition of the IADR, Vol.2016, 154, Seoul, Jun. 2016.
6.
Makoto Fukui, Kaname Miki, Kitamura Masayasu, Hayashida Hideaki, Kawasaki Koji, Rita Cristina Orihuela Campos, Naofumi Tamaki, Maeda Takahiro, Saito Toshiyuki and Hiro-O Ito : Porphyromonas gingivalis infection may affect anti-atheroschlerotic antibody to phosphorylcholine., The 12th International Conference of Asia Academy of Preventive Dentistry, May 2016.
7.
Akari Watanabe, Naofumi Tamaki, Susumu Kokeguchi and Miwa Matsuyama : A survey of Lasioderma serricorne (FABRICIUS) in Japanese Dental Clinics, 93rd General Session & Exhibition of the IADR, Vol.2015, 147, Boston, Mar. 2015.
8.
Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui, Kaname Miki and Hiro-O Ito : Balance is the key: resveratrol revert oxidative stress conditions in gingival fibroblasts, 第93回IADR, Mar. 2015.
9.
Naofumi Tamaki, Makoto Fukui, Hiro-O Ito and Kokeguchi Susumu : Salivary Antioxidant Activity, Cytokines and Periodontitis: The Nagasaki Island Study, International Association for Dental Research-Asia Pacific Region(IADR-APR), Bangkok, Aug. 2013.
10.
Akari Watanabe, Naofumi Tamaki, Miwa Matsuyama and Susumu Kokeguchi : Bacterial Contamination of Dental Units used by Dental Hygiene Students, International Association for Dental Research-Asia Pacific Region(IADR-APR), Bangkok, Aug. 2013.
11.
Akari Watanabe, Naofumi Tamaki, Norito Sato and Susumu Kokeguchi : Infection Control Awareness among American and Japanese Dental Hygienist Students, IADR, Seattle, Mar. 2013.
12.
Naofumi Tamaki and Hiro-O Ito : Relationship between plasma reactive oxygen species and progression of periodontitis, ICAAPD, ウランバートル, Feb. 2013.
13.
Naofumi Tamaki : Hydrogen-rich water prevents progression of non-alcoholic steatohepatitis and accompanying hepatocarcinogenesis compared with pioglitazone in mice model, UEGW, Oct. 2012.
14.
Makoto Fukui, Kaname Miki, Naofumi Tamaki and Hiro-O Ito : Relationship between atherosclerosis and anti-phosphorylcholine antibodies in saliva and plasma, ICAAPD, ウランバートル, Sep. 2012.
15.
Naofumi Tamaki and Hiro-O Ito : Relationship between plasma reactive oxygen species and progression of periodontitis, ICAAPD, ウランバートル, Sep. 2012.
16.
Naofumi Tamaki, Makoto Fukui and Hiro-O Ito : Levels of Serum Markers of Oxidative Stress and Antibodies against Periodontal Pathogens in Community-dwelling Adults with and without Periodontitis: Goto Study, International Conference on Porphyromonas gingivalis and Related Bacterial Species, Nagasaki, Aug. 2012.
17.
Naofumi Tamaki : Hydrogen-rich water prevents initiation of atherosclerosis induced by periodontitis in the rat, Europerio7, Wien, Jun. 2012.
18.
Naofumi Tamaki : The relationship of periodontal disease to oxidative stress and life style, National Scientific Seminar in Periodontics, Makassar, May 2012.
19.
Akari Watanabe, Norito Sato, Naofumi Tamaki and Susumu Kokeguchi : Evaluation of contamination on dental PPE using an ATP-bioluminescence method., International Union of Microbiological Societies 2011, Sapporo, Sep. 2011.
20.
Akari Watanabe, Sato Norito, Naofumi Tamaki, Tanimoto Ichiro, Maeda Hiroshi, Takashiba Shogo and Kokeguchi Susumu : Monitoring microbial contamination on namecard-holders to prevent nosocomial infections., 87th General Session & Exhibition of the IADR, Miami, Mar. 2009.
21.
Akari Watanabe, Naofumi Tamaki, Ichiro Tanimoto, Hiroshi Maeda, Norito Sato, Shogo Takashiba, Susumu Kokeguchi and Kazuhiro Fukui : Molecular Analysis for Bacterial Contamination in Dental Unit Water Line., 86th General Session & Exhibition of the IADR, Toronto, Jul. 2008.
Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui and Hiro-O Ito : Polyphenols reduce collateral damage in HGFs induced by oxidative stress, 44th Annual Meeting of the Japanese Society for Immunology, Nov. 2015.
13.
Makoto Fukui, Rita Cristina Orihuela Campos, Naofumi Tamaki and Hiro-O Ito : Influence of infection with Porphyromonas gingivalis on relationship between atherosclerosis and antiphosphorylcholine antibody, 44th Annual Meeting of the Japanese Society for Immunology, Nov. 2015.
14.
Makoto Fukui, Kaname Miki, K Kitamura, H Hayashida, K Kawasaki, Rita Cristina Orihuela Campos, Naofumi Tamaki, T Maeda, T Saito and Hiro-O Ito : Atherosclerosis-preventive activity of anti-phosphorylcholine antibody may be interfered by infection with Porphyromonas gingivalis : The Nagasaki Islands study, 63rd Annual Meeting of the Japanese Association of Dental Research, Oct. 2015.
Makoto Fukui, Kaname Miki, Rita Cristina Orihuela Campos, Naofumi Tamaki and Hiro-O Ito : Levels of various cytokines in human saliva from generally healthy individuals., 62nd Annual Meeting of the Japanese Association for Dental Research, Dec. 2014.
Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui, Kaname Miki and Hiro-O Ito : Effects of three different antioxidants on human gingival fibroblasts exposed to oxidative stress, 2014 Tokushima Bioscience retreat, Sep. 2014.
Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui, Kaname Miki and Hiro-O Ito : Effects of three antioxidant compounds on human gingival fibroblasts under oxidative stress, 第63回 口腔衛生学会総会, May 2014.
33.
Naofumi Tamaki : Relationship between plasma reactive oxygen metabolites and periodontitis progression: A prospective cohort study, 17th SFRRI, Mar. 2014.
Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui, Kaname Miki and Hiro-O Ito : Effects of different antioxidants on human gingival fibroblasts under oxidative stress, 第5回心・血管クラスターミニリトリート, Jan. 2014.
Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui, Kaname Miki and Hiro-O Ito : Effects of different antioxidants on the biological properties of human gingival fibroblasts response toward ROS, Tokushima Bioscience retreat, Sep. 2013.
42.
Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui, Kaname Miki and Hiro-O Ito : Real time xCELLigence analysis of antioxidant agents on human gingival fibroblast cells viability, 第62回日本口腔衛生学会総会, May 2013.
43.
玉木 直文 : Stage of hepatocellular carcinoma is associated with periodontitis, 第62回日本口腔衛生学会総会, 2013年5月.
SA Mulyatno, Naofumi Tamaki, Makoto Fukui and Hiro-O Ito : Mucosal adjuvanticity of bacterial lipopolysaccharide in compared with cholera toxin, Proceedings of the Japanese Society for Immunology, Dec. 2012.