label: url

en: Type I-D CRISPR system-mediated genome editing in plants

en: Humana New York

ja: Humana New York


en: CRISPR/Cas9 tools for multiplex genome editing in crops

en: Springer, Singapore

ja: Springer, Singapore


en: Plant Genome Editing

ja: Plant Genome Editing

en: CAB International

ja: CAB International


en: Genome Editing in Apple

ja: Genome Editing in Apple

en: Springer

ja: Springer


en: CRISPR-Cas type Iシステムを利用した新しいゲノム編集技術の開発

ja: CRISPR-Cas type Iシステムを利用した新しいゲノム編集技術の開発

en: 羊土社

ja: 羊土社


en: 第9章 高等動植物に利用可能な新規ゲノム編集ツールの開発

ja: 第9章 高等動植物に利用可能な新規ゲノム編集ツールの開発

en: 株式会社シーエムシー出版

ja: 株式会社シーエムシー出版


en: 第2章DNA編集技術 1. CRISPR/Casによるゲノム編集技術-総論

ja: 第2章DNA編集技術 1. CRISPR/Casによるゲノム編集技術-総論

en: 株式会社エヌ・ティー・エス

ja: 株式会社エヌ・ティー・エス


en: 第2章17 植物でのゲノム編集」完全版ゲノム編集実験スタンダード (実験医学別冊) (分担執筆)山本卓，佐久間哲史編集

en: YODOSHA CO., LTD.

ja: 株式会社 羊土社


en: 「第III部18章 ゲノム編集」 基礎から学ぶ植物代謝生化学(分担執筆)士反伸和，水谷正治，杉山暁史編集


en: Crop Breeding Using CRISPR/Cas9, in Crop Improvement Through Microbial Biotechnology

en: Elsevier

ja: Elsevier


en: Genome editing to improve abiotic stress responses in plants. in Gene Editing in Plants, Progress in Molecular Biology and Translational Science, ELSEVIER


en: 「第11章 植物でのゲノム編集」論文だけではわからないゲノム編集成功の秘訣Q&A


en: 「人工ヌクレアーゼを用いた植物の遺伝子変異導入技術への応用展開」進化するゲノム編集技術

en: エヌ・ティー・エス

ja: エヌ・ティー・エス


en: Genome editing in higher plants. Targeted genome editing using site-specific nucleases

en: Genome editing with engineered nucleases (``GEEN'') has been developed as one of the effective genetic engineering methods using `molecular scissors', or artificially engineered nucleases to digest the DNAs at the desired locations in the genome of various organisms including plant species. The DNA binding domains, Zinc finger (ZF) proteins, have firstly been used for the plant genome editing tool as the designed ZF nucleases (ZFNs), and now TAL-effectors (TALE) and the RNA-DNA recognition system, CRISPR/Cas, instead of protein-DNA recognition are used for the targeted gene modifications in not only model plants but also crop species as powerful genome editing tools. The key for genome editing is the target gene-specific double-stranded DNA break (DSB) by using the designed endonucleases, in which the site-directed mutagenesis via nonhomologous end joining (NHEJ) repair pathway and/or gene targeting via homologous recombination (HR), can efficiently be occurred at the specific sites in genome. This chapter provides the overview of the recent advances in genome editing technologies to understand the more insights into current plant molecular biology and breeding technology.


en: イネのジーンタ-ゲティング

ja: イネのジーンタ-ゲティング

en: Kagaku-Dojin Publishing Company,INC

ja: 株式会社 化学同人

en: ジーンターゲティング(GT)とはゲノム上の標的遺伝子を相同組換え機構を利用し外来DNAと置き換える技術である．GTがおきた細胞を高頻度で選抜する一つのアプローチとしてポジティブ選抜マーカーとネガティブ選抜マーカーを組み合わせた方法が用いられる．本稿では，このポジティブ・ネガティブ選抜方法を用いたイネのGT実験例を具体的に述べた．


en: Plant environmental stress responses for survival and biomass enhancement Climate change and abiotic stress tolerance

en: Wiley-VCH

ja: Wiley-VCH


en: Site-directed mutagenesis in higher plants.

en: The Smiling Hippo

ja: The Smiling Hippo

en: Double-strand DNA breaks (DSBs) are the most threatening type of DNA damages in living cells. For the maintenance of genome integrity and cell survival, it is critical that cells repair such breaks accurately and faithfully. Cells possess several DNA repair pathways to deal with DSBs. Genetic and biochemical studies have provided considerable data on the genes and their encoded proteins involved in these repair systems and their functions. The completion of genome sequencing of Arabidopsis and several major crops has greatly facilitated the search and study of genes involved in DNA repair. Several Arabidopsis mutants that are deficient in homologous recombination (HR, Box 1) and non-homologous end joining (NHEJ, Box 1) have been identified


en: シロイヌナズナの部位特異的変異導入法, 形質転換プロトコル(植物編)(バイオサイエンス アドバンスドマニュアルシリーズ)編

en: Tokyo Kagaku Dozin C., Ltd.

ja: 株式会社 東京化学同人


en: Abiotic stress responses in plants

ja: Abiotic stress responses in plants

en: Nova Science Publishers. USA

ja: Nova Science Publishers. USA


en: Plant abiotic stress responses and nutrients


en: Abiotic stress responses in woody plants; molecular perspective in engineering woody plant tolerance to abiotic stress and enhance biomass


en: Chapter 19 Homologous recombination in higher plants.

en: Springer

en: Homologous recombination (HR) is one of two major repair systems for double-stranded DNA breaks (DSBs). A deficiency in either repair pathway is highly toxic to eukaryotes. HR also forms the basis of the mechanism of programmed genetic recombination, such as meiotic recombination, thus producing genetic variation. Indeed, breeding programs depend heavily on the crossover events of meiotic HR. Genetic and biochemical studies have provided evidence on the proteins involved in HR and their functions in higher plants. These include several HR repair-gene-deficient Arabidopsis mutants showing hypersensitivity to DSB-inducing agents. Unlike vertebrates, however, with a few exceptions these mutants do not show embryonic lethality or severe growth defects. This could represent a distinct advantage of plant systems for the study of HR. In this chapter, recent advances in the HR mechanism in eukaryotes are discussed, including signal transduction in higher plants in relation to HR repair.


en: Agrobacterium-mediated transformation of poplar and using antisense RNA method to inhibit lignin biosynthesis.

en: Uni Publisher

ja: Uni Publisher

en: We have cloned and characterized several cDNAs for peroxidases from hybrid aspen, Populus kitakamiensis. To analyze the function of these peroxidases, an efficient method was established for transformation of the poplar hybrid Populus kitakamiensis (Populus sieboldii × Populus gradidentata) using a binary disarmed strain of Agrobacterium tumefaciens LBA4404 and Ti-binary vectors. We altered the expressin of a peroxidase isozyme by introducing an identified genomic gene for a peroxidase (prxA1) under the control of the CaMV35S promoter. Transgenic poplars obtained by introducing the chimeric peroxidase gene (CaMV35S promoter-prxA1) were shown to have an increase in total peroxidase activity that was accounted for by the specific overproduction of the peroxidase isozyme (PrxA1). From this study, the anionic peroxidase isozyme encoded by the identified genomic gene, prxA1, was demonstrated to be the anionic peroxidase isozyme with a pI of 4.4 among various isozymes of poplar peroxidase. On the basis of this assignment, we characterized the tissue-specific and UV-light-inducible regulation of expression of this isozyme.