{"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/118834","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/36834667","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=394204","label":"url"}],"paper_title":{"en":"Effects of Candidalysin Derived from Candida albicans on the Expression of Pro-Inflammatory Mediators in Human Gingival Fibroblasts","ja":"Effects of Candidalysin Derived from Candida albicans on the Expression of Pro-Inflammatory Mediators in Human Gingival Fibroblasts"},"authors":{"en":[{"name":"Nishikawa Yasufumi"},{"name":"Tomotake Yoritoki"},{"name":"Kawano Hiromichi"},{"name":"Naruishi Koji"},{"name":"Kido Jun-ichi"},{"name":"Hiroshima Yuka"},{"name":"Murakami Akikazu"},{"name":"Ichikawa Tetsuo"},{"name":"Yumoto Hiromichi"}],"ja":[{"name":"西川 泰史"},{"name":"友竹 偉則"},{"name":"川野 弘道"},{"name":"成石 浩司"},{"name":"木戸 淳一"},{"name":"廣島 佑香"},{"name":"村上 明一"},{"name":"市川 哲雄"},{"name":"湯本 浩通"}]},"publication_date":"2023-02-07","publication_name":{"en":"International Journal of Molecular Sciences","ja":"International Journal of Molecular Sciences"},"volume":"Vol.24","number":"No.4","starting_page":"3256","ending_page":"3256","languages":["eng"],"referee":true,"identifiers":{"doi":["10.3390/ijms24043256"],"issn":["1422-0067"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/36579753","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=394011","label":"url"}],"paper_title":{"en":"Lipocalin 2, synthesized using a cell-free protein synthesis system and encapsulated into liposomes, inhibits the adhesion of Porphyromonas gingivalis to human oral epithelial cells.","ja":"Lipocalin 2, synthesized using a cell-free protein synthesis system and encapsulated into liposomes, inhibits the adhesion of Porphyromonas gingivalis to human oral epithelial cells."},"authors":{"en":[{"name":"Kido Jun-ichi"},{"name":"Hiroshima Yuka"},{"name":"Kido Rie"},{"name":"Yoshida Kaya"},{"name":"Inagaki Yuji"},{"name":"Naruishi Koji"},{"name":"Kajimoto Kazuaki"},{"name":"Kataoka Masatoshi"},{"name":"Shinohara Yasuo"},{"name":"Yumoto Hiromichi"}],"ja":[{"name":"木戸 淳一"},{"name":"廣島 佑香"},{"name":"木戸 理恵"},{"name":"吉田 賀弥"},{"name":"稲垣 裕司"},{"name":"成石 浩司"},{"name":"Kajimoto Kazuaki"},{"name":"片岡 正俊"},{"name":"篠原 康雄"},{"name":"湯本 浩通"}]},"publication_date":"2022-12-29","publication_name":{"en":"Journal of Periodontal Research","ja":"Journal of Periodontal Research"},"volume":"Vol.58","number":"No.2","starting_page":"262","ending_page":"273","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1111/jre.13088"],"issn":["1600-0765"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/117989","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/36359741","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=392799","label":"url"}],"paper_title":{"en":"Biological Roles of Fibroblasts in Periodontal Diseases.","ja":"Biological Roles of Fibroblasts in Periodontal Diseases."},"authors":{"en":[{"name":"Naruishi Koji"}],"ja":[{"name":"成石 浩司"}]},"description":{"en":"Periodontal diseases include periodontitis and gingival overgrowth. Periodontitis is a bacterial infectious disease, and its pathological cascade is regulated by many inflammatory cytokines secreted by immune or tissue cells, such as interleukin-6. In contrast, gingival overgrowth develops as a side effect of specific drugs, such as immunosuppressants, anticonvulsants, and calcium channel blockers. Human gingival fibroblasts (HGFs) are the most abundant cells in gingival connective tissue, and human periodontal ligament fibroblasts (HPLFs) are located between the teeth and alveolar bone. HGFs and HPLFs are both crucial for the remodeling and homeostasis of periodontal tissue, and their roles in the pathogenesis of periodontal diseases have been examined for 25 years. Various responses by HGFs or HPLFs contribute to the progression of periodontal diseases. This review summarizes the biological effects of HGFs and HPLFs on the pathogenesis of periodontal diseases.","ja":"Periodontal diseases include periodontitis and gingival overgrowth. Periodontitis is a bacterial infectious disease, and its pathological cascade is regulated by many inflammatory cytokines secreted by immune or tissue cells, such as interleukin-6. In contrast, gingival overgrowth develops as a side effect of specific drugs, such as immunosuppressants, anticonvulsants, and calcium channel blockers. Human gingival fibroblasts (HGFs) are the most abundant cells in gingival connective tissue, and human periodontal ligament fibroblasts (HPLFs) are located between the teeth and alveolar bone. HGFs and HPLFs are both crucial for the remodeling and homeostasis of periodontal tissue, and their roles in the pathogenesis of periodontal diseases have been examined for 25 years. Various responses by HGFs or HPLFs contribute to the progression of periodontal diseases. This review summarizes the biological effects of HGFs and HPLFs on the pathogenesis of periodontal diseases."},"publication_date":"2022-10-24","publication_name":{"en":"Cells","ja":"Cells"},"volume":"Vol.11","number":"No.21","starting_page":"3345","ending_page":"3345","languages":["eng"],"referee":true,"identifiers":{"doi":["10.3390/cells11213345"],"issn":["2073-4409"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=383760","label":"url"}],"paper_title":{"en":"Dental students' awareness after clinical training between dental treatment and systemic health: A questionnaire-based survey","ja":"Dental students' awareness after clinical training between dental treatment and systemic health: A questionnaire-based survey"},"authors":{"en":[{"name":"Naruishi Koji"},{"name":"Wada -Mihara Chie"},{"name":"Oishi Keiji"},{"name":"Nagata Toshihiko"}],"ja":[{"name":"成石 浩司"},{"name":"美原(和田) 智恵"},{"name":"大石 慶二"},{"name":"永田 俊彦"}]},"publication_date":"2022-01-12","publication_name":{"en":"Frontiers in Dental Medicine","ja":"Frontiers in Dental Medicine"},"languages":["eng"],"referee":true,"identifiers":{"doi":["10.3389/fdmed.2021.740441"],"issn":["2673-4915"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/116745","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/33498415","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=379180","label":"url"}],"paper_title":{"en":"Gan-Lu-Yin (Kanroin), traditional Chinese herbal extracts, reduces osteoclast differentiation in vitro and prevents alveolar bone resorption in rat experimental periodontitis","ja":"Gan-Lu-Yin (Kanroin), traditional Chinese herbal extracts, reduces osteoclast differentiation in vitro and prevents alveolar bone resorption in rat experimental periodontitis"},"authors":{"en":[{"name":"Inagaki Yuji"},{"name":"Kido Jun-ichi"},{"name":"Nishikawa Yasufumi"},{"name":"Kido Rie"},{"name":"Sakamoto Eijiro"},{"name":"Bandou Mika"},{"name":"Naruishi Koji"},{"name":"Nagata Toshihiko"},{"name":"Yumoto Hiromichi"}],"ja":[{"name":"稲垣 裕司"},{"name":"木戸 淳一"},{"name":"西川 泰史"},{"name":"木戸 理恵"},{"name":"坂本 英次郎"},{"name":"板東 美香"},{"name":"成石 浩司"},{"name":"永田 俊彦"},{"name":"湯本 浩通"}]},"description":{"en":"Gan-Lu-Yin (GLY), a traditional Chinese herbal medicine, shows therapeutic effects on periodontitis, but that mechanism is not well known. This study aims to clarify the precise mechanism by investigating the inhibitory effects of GLY extracts on osteoclastogenesis in vitro and on bone resorption in periodontitis in vivo. RAW264.7 cells are cultured with soluble receptor activator of nuclear factor-kappa B (sRANKL) and GLY extracts (0.01-1.0 mg/mL), and stained for tartrate-resistant acid phosphatase (TRAP) to evaluate osteoclast differentiation. Experimental periodontitis is induced by placing a nylon ligature around the second maxillary molar in rats, and rats are administered GLY extracts (60 mg/kg) daily for 20 days. Their maxillae are collected on day 4 and 20, and the levels of alveolar bone resorption and osteoclast differentiation are estimated using micro-computed tomography (CT) and histological analysis, respectively. In RAW264.7 cells, GLY extracts significantly inhibit sRANKL-induced osteoclast differentiation at a concentration of more than 0.05 mg/mL. In experimental periodontitis, administering GLY extracts significantly decreases the number of TRAP-positive osteoclasts in the alveolar bone on day 4, and significantly inhibits the ligature-induced bone resorption on day 20. These results show that GLY extracts suppress bone resorption by inhibiting osteoclast differentiation in experimental periodontitis, suggesting that GLY extracts are potentially useful for oral care in periodontitis.","ja":"Gan-Lu-Yin (GLY), a traditional Chinese herbal medicine, shows therapeutic effects on periodontitis, but that mechanism is not well known. This study aims to clarify the precise mechanism by investigating the inhibitory effects of GLY extracts on osteoclastogenesis in vitro and on bone resorption in periodontitis in vivo. RAW264.7 cells are cultured with soluble receptor activator of nuclear factor-kappa B (sRANKL) and GLY extracts (0.01-1.0 mg/mL), and stained for tartrate-resistant acid phosphatase (TRAP) to evaluate osteoclast differentiation. Experimental periodontitis is induced by placing a nylon ligature around the second maxillary molar in rats, and rats are administered GLY extracts (60 mg/kg) daily for 20 days. Their maxillae are collected on day 4 and 20, and the levels of alveolar bone resorption and osteoclast differentiation are estimated using micro-computed tomography (CT) and histological analysis, respectively. In RAW264.7 cells, GLY extracts significantly inhibit sRANKL-induced osteoclast differentiation at a concentration of more than 0.05 mg/mL. In experimental periodontitis, administering GLY extracts significantly decreases the number of TRAP-positive osteoclasts in the alveolar bone on day 4, and significantly inhibits the ligature-induced bone resorption on day 20. These results show that GLY extracts suppress bone resorption by inhibiting osteoclast differentiation in experimental periodontitis, suggesting that GLY extracts are potentially useful for oral care in periodontitis."},"publication_date":"2021-01-20","publication_name":{"en":"Journal of Clinical Medicine","ja":"Journal of Clinical Medicine"},"volume":"Vol.10","number":"No.3","starting_page":"386","ending_page":"386","languages":["eng"],"referee":true,"identifiers":{"doi":["10.3390/jcm10030386"],"issn":["2077-0383"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://search.jamas.or.jp/link/ui/2021171621","label":"url"},{"@id":"https://cir.nii.ac.jp/crid/1390849931318242944/","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=373139","label":"url"}],"paper_title":{"en":"Inhibitory Effects of Sudachitin on the Production of Inflammation-related Molecules in Human Gingival Fibroblasts Cultured under High Glucose Conditions","ja":"高グルコース条件下における歯肉線維芽細胞のカルプロテクチン誘導性炎症関連因子の産生におけるスダチチンの抑制効果"},"authors":{"en":[{"name":"Nishikawa Yasufumi"},{"name":"Naruishi Koji"},{"name":"Kido Jun-ichi"},{"name":"Yumoto Hiromichi"}],"ja":[{"name":"西川 泰史"},{"name":"成石 浩司"},{"name":"木戸 淳一"},{"name":"湯本 浩通"}]},"publication_date":"2020-12-25","publication_name":{"en":"The Japanese Journal of Conservative Dentistry","ja":"日本歯科保存学雑誌"},"volume":"Vol.63","number":"No.6","starting_page":"503","ending_page":"511","languages":["jpn"],"referee":true,"identifiers":{"doi":["10.11471/shikahozon.63.503"],"issn":["2188-0808"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/115292","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=367257","label":"url"}],"paper_title":{"en":"Disequilibrium of Plasma Protease/Anti-Protease Due to Severe Periodontal Disease Contributes to Human Subarachnoid Hemorrhage","ja":"Disequilibrium of Plasma Protease/Anti-Protease Due to Severe Periodontal Disease Contributes to Human Subarachnoid Hemorrhage"},"authors":{"en":[{"name":"Yoshioka Shotaroh"},{"name":"Miyamoto Takeshi"},{"name":"Satomi Junichiro"},{"name":"Tada Yoshiteru"},{"name":"Yagi Kenji"},{"name":"Shimada Kenji"},{"name":"Naruishi Koji"},{"name":"Shikata Eiji"},{"name":"Yamaguchi Izumi"},{"name":"Yamaguchi Tadashi"},{"name":"Korai Masaaki"},{"name":"Okayama Yoshihiro"},{"name":"Harada Masafumi"},{"name":"Kitazato Keiko"},{"name":"Kanematsu Yasuhisa"},{"name":"Nagahiro Shinji"},{"name":"Takagi Yasushi"}],"ja":[{"name":"?岡 正太郎"},{"name":"宮本 健志"},{"name":"里見 淳一郎"},{"name":"多田 恵曜"},{"name":"八木 謙次"},{"name":"島田 健司"},{"name":"成石 浩司"},{"name":"四方 英二"},{"name":"山口 泉"},{"name":"山口 真司"},{"name":"高麗 雅章"},{"name":"Okayama Yoshihiro"},{"name":"原田 雅史"},{"name":"北里 慶子"},{"name":"兼松 康久"},{"name":"永廣 信治"},{"name":"髙木 康志"}]},"publication_date":"2020-07-23","publication_name":{"en":"Neurosurgery Open","ja":"Neurosurgery Open"},"volume":"Vol.1","number":"No.3","starting_page":"1","ending_page":"9","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1093/neuopn/okaa007"],"issn":["2633-0873"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/114681","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/32170733","label":"url"},{"@id":"https://www.scopus.com/record/display.url?eid=2-s2.0-85081730917&origin=inward","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=363586","label":"url"}],"paper_title":{"en":"Advanced glycation end-products increase lipocalin 2 expression in human oral epithelial cells.","ja":"Advanced glycation end-products increase lipocalin 2 expression in human oral epithelial cells."},"authors":{"en":[{"name":"Kido Rie"},{"name":"Hiroshima Yuka"},{"name":"Kido Jun-ichi"},{"name":"Ikuta Takahisa"},{"name":"Sakamoto Eijiro"},{"name":"Inagaki Yuji"},{"name":"Naruishi Koji"},{"name":"Yumoto Hiromichi"}],"ja":[{"name":"木戸 理恵"},{"name":"廣島 佑香"},{"name":"木戸 淳一"},{"name":"生田 貴久"},{"name":"坂本 英次郎"},{"name":"稲垣 裕司"},{"name":"成石 浩司"},{"name":"湯本 浩通"}]},"publication_date":"2020-03-13","publication_name":{"en":"Journal of Periodontal Research","ja":"Journal of Periodontal Research"},"volume":"Vol.55","number":"No.4","starting_page":"539","ending_page":"550","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1111/jre.12741"],"issn":["1600-0765"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://ci.nii.ac.jp/naid/130007804952/","label":"url"},{"@id":"https://cir.nii.ac.jp/crid/1390002184879112064/","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=363276","label":"url"}],"paper_title":{"en":"Evaluation of educational effects of periodontology by lectures and phantom training in dental students","ja":"講義および実習試験の評価による歯周病学教育のあり方の考察"},"authors":{"en":[{"name":"Naruishi Koji"},{"name":"Sakamoto Eijiro"},{"name":"Ikuta Takahisa"},{"name":"Kido Rie"},{"name":"Kido Jun-ichi"},{"name":"Yumoto Hiromichi"}],"ja":[{"name":"成石 浩司"},{"name":"坂本 英次郎"},{"name":"生田 貴久"},{"name":"木戸 理恵"},{"name":"木戸 淳一"},{"name":"湯本 浩通"}]},"description":{"en":"
Purpose: In the undergraduate educational curriculum of dentistry, there are two main teaching/learning methods for dental students before clinical training: traditional lectures and phantom training. The purpose of this study was to explore effective educational systems for periodontology by examining the test results of both lectures and phantom training.
Methods: Both a written test and a practical test of periodontology were administered to students of dentistry. A total of 43 dental students of Tokushima University Dental School were recruited. Written tests were performed before and after the lectures on periodontology. A practical test of scaling and root planing was performed after phantom training. Associations between the results of the written test and practical test were analyzed statistically. P values of less than 0.05 were considered statistically significant.
Results: The results of the written tests after lectures were higher than those before lectures. A significant positive correlation was found between the results of the written test and practical test.
Conclusion: In the students' education on periodontology, the results of the practical test were correlated statistically to the results of the written test. These findings may contribute to establishing effective educational systems for periodontology in dentistry.
","ja":"目的 : 一般的に, 臨床実習前の歯学部生に対する歯周病学の教育は, 座学講義と基礎 (模型) 実習を主体とする. 本研究の目的は, 歯周病学の講義および実習の評価試験の結果の関連性を調べ, 将来の歯学部生に対する歯周病学教育のあり方について考察することである.
方法 : 2018年度後期から2019年度前期に徳島大学歯学部に在籍し, 歯周病学を履修する歯学部3∼4年次生43名を対象とした. 歯周病学の講義前後に当該分野に関する筆記試験 (5肢2択形式) を行い, さらに歯周病学の基礎 (模型) 実習の終了時に, 顎模型を用いてスケーリング・ルートプレーニングの実習試験を行った. 講義による学生の理解度・習得度の評価は, カイ二乗検定を用いて判定した. 筆記試験および実習試験の結果の関連性は, スピアマンの順位相関検定を用いて判定した. また, 筆記試験の点数を25点満点で9点以下, 10∼12点, 13点以上の3群に分類し, 各群間の実習試験点数の差異の有無について, ANOVA-Tukey HSD解析を用いて判定した. なお, p値が0.05未満を有意差ありと判定した.
結果 : 歯周病学の講義後の筆記試験の点数は, 出題内容によって差はあるものの, 講義前と比較しておおむね有意に上昇した. 筆記試験の結果と実技試験の結果は, 有意に相関した (r=0.34, p=0.025). また筆記試験の点数が9点以下の群における実習試験の点数は, 筆記試験の点数が10∼12点, 13点以上の群と比較して有意に低かった (p<0.05). 一方, 筆記試験の点数が10∼12点と13点以上の群間における実習試験の点数に有意差は認められなかった (p=0.76).
結論 : 臨床実習前の歯周病学の学生教育において, 筆記試験の点数と基礎 (模型) 実習試験の点数は有意に相関した. このことは, 将来の効果的な歯周病学の教育システム構築の一助となる可能性を示唆する.
"},"publication_date":"2020-03-02","publication_name":{"en":"The Japanese Journal of Conservative Dentistry","ja":"日本歯科保存学雑誌"},"volume":"Vol.63","number":"No.1","starting_page":"22","ending_page":"29","languages":["jpn"],"referee":true,"identifiers":{"doi":["10.11471/shikahozon.63.22"],"issn":["0387-2343"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/114641","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/32149126","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=363582","label":"url"}],"paper_title":{"en":"S100A9 Increases IL-6 and RANKL Expressions through MAPKs and STAT3 Signaling Pathways in Osteocyte-Like Cells.","ja":"S100A9 Increases IL-6 and RANKL Expressions through MAPKs and STAT3 Signaling Pathways in Osteocyte-Like Cells."},"authors":{"en":[{"name":"Takagi Ryosuke"},{"name":"Sakamoto Eijiro"},{"name":"Kido Jun-ichi"},{"name":"Inagaki Yuji"},{"name":"Hiroshima Yuka"},{"name":"Naruishi Koji"},{"name":"Yumoto Hiromichi"}],"ja":[{"name":"高木 亮輔"},{"name":"坂本 英次郎"},{"name":"木戸 淳一"},{"name":"稲垣 裕司"},{"name":"廣島 佑香"},{"name":"成石 浩司"},{"name":"湯本 浩通"}]},"publication_date":"2020-02-19","publication_name":{"en":"BioMed Research International","ja":"BioMed Research International"},"volume":"Vol.2020","number":"No.7149408","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1155/2020/7149408"],"issn":["2314-6141"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/113773","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/31197658","label":"url"},{"@id":"https://www.scopus.com/record/display.url?eid=2-s2.0-85067639107&origin=inward","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=352492","label":"url"}],"paper_title":{"en":"Relationship of oral conditions to the incidence of infective endocarditis in periodontitis patients with valvular heart disease: a cross-sectional study.","ja":"Relationship of oral conditions to the incidence of infective endocarditis in periodontitis patients with valvular heart disease: a cross-sectional study."},"authors":{"en":[{"name":"Ninomiya Masami"},{"name":"Hashimoto Mari"},{"name":"Yamanouchi Kouji"},{"name":"Fukumura Yoshiaki"},{"name":"Nagata Toshihiko"},{"name":"Naruishi Koji"}],"ja":[{"name":"二宮 雅美"},{"name":"橋本 万里"},{"name":"Yamanouchi Kouji"},{"name":"Fukumura Yoshiaki"},{"name":"永田 俊彦"},{"name":"成石 浩司"}]},"description":{"en":"The patients with IE had fewer remaining teeth, more advanced bone resorption compared with those of patients without IE. These findings suggest a possible association between the occurrence of IE and periodontal infection.","ja":"No significant differences were observed between patients with or without VHD in oral conditions. A significant increase in the percentage of alveolar bone loss in VHD patients with IE was observed compared with that of patients without IE. The ratio of both Porphyromonas gingivalis (Pg) IgG titer > 1.68 and Pg fimA type II genotype in patients with IE was significantly higher than in patients without IE. There was a significant correlation between the occurrence of IE and clinical oral findings (number of remaining teeth: OR, 0.17; rate of alveolar bone loss > 40%: OR, 11.8)."},"publication_date":"2020-02-01","publication_name":{"en":"Clinical Oral Investigations","ja":"Clinical Oral Investigations"},"volume":"Vol.24","number":"No.2","starting_page":"833","ending_page":"840","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1007/s00784-019-02973-2"],"issn":["1436-3771"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/115657","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/31968635","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=362811","label":"url"}],"paper_title":{"en":"Carotenoids and Periodontal Infection.","ja":"Carotenoids and Periodontal Infection."},"authors":{"en":[{"name":"Naruishi Koji"}],"ja":[{"name":"成石 浩司"}]},"description":{"en":") is associated with increased atherosclerosis, diabetes mellitus, and other systemic diseases through blood stream. On the other hand, carotenoids belong among phytochemicals that are responsible for different colors of the foods. It is important to examine whether carotenoids are effective to the inhibition of periodontal infection/inflammation cascades. This review summarizes the advanced state of knowledge about suppression of periodontal infection by several carotenoids. A series of findings suggest that carotenoids intake may provide novel strategy for periodontitis treatment, although further study will be needed.","ja":") is associated with increased atherosclerosis, diabetes mellitus, and other systemic diseases through blood stream. On the other hand, carotenoids belong among phytochemicals that are responsible for different colors of the foods. It is important to examine whether carotenoids are effective to the inhibition of periodontal infection/inflammation cascades. This review summarizes the advanced state of knowledge about suppression of periodontal infection by several carotenoids. A series of findings suggest that carotenoids intake may provide novel strategy for periodontitis treatment, although further study will be needed."},"publication_date":"2020-01-20","publication_name":{"en":"Nutrients","ja":"Nutrients"},"volume":"Vol.12","number":"No.1","starting_page":"E269","ending_page":"E269","languages":["eng"],"referee":true,"identifiers":{"doi":["10.3390/nu12010269"],"issn":["2072-6643"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/114673","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/31619000","label":"url"},{"@id":"https://www.scopus.com/record/display.url?eid=2-s2.0-85073474436&origin=inward","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=362486","label":"url"}],"paper_title":{"en":"6-Shogaol inhibits advanced glycation end-products-induced IL-6 and ICAM-1 expression by regulating oxidative responses in human gingival fibroblasts","ja":"6-Shogaol inhibits advanced glycation end-products-induced IL-6 and ICAM-1 expression by regulating oxidative responses in human gingival fibroblasts"},"authors":{"en":[{"name":"Nonaka Kohei"},{"name":"Bandou Mika"},{"name":"Sakamoto Eijiro"},{"name":"Inagaki Yuji"},{"name":"Naruishi Koji"},{"name":"Yumoto Hiromichi"},{"name":"Kido Jun-ichi"}],"ja":[{"name":"野中 康平"},{"name":"板東 美香"},{"name":"坂本 英次郎"},{"name":"稲垣 裕司"},{"name":"成石 浩司"},{"name":"湯本 浩通"},{"name":"木戸 淳一"}]},"description":{"en":"Advanced glycation end-products (AGEs) cause diabetes mellitus (DM) complications and accumulate more highly in periodontal tissues of patients with periodontitis and DM. AGEs aggravate periodontitis with DM by increasing the expression of inflammation-related factors in periodontal tissues. 6-Shogaol, a major compound in ginger, has anti-inflammatory and anti-oxidative activities. However, the influence of shogaol on DM-associated periodontitis is not well known. In this study, the effects of 6-shogaol on AGEs-induced oxidative and anti-oxidative responses, and IL-6 and ICAM-1 expression in human gingival fibroblasts (HGFs) were investigated. When HGFs were cultured with 6-shogaol and AGEs, the activities of reactive oxygen species (ROS) and antioxidant enzymes (heme oxygenase-1 [HO-1] and NAD(P)H quinone dehydrogenase 1 [NQO1]), and IL-6 and ICAM-1 expressions were investigated. RAGE expression and phosphorylation of MAPKs and NF-κB were examined by western blotting. 6-Shogaol significantly inhibited AGEs-induced ROS activity, and increased HO-1 and NQO1 levels compared with the AGEs-treated cells. The AGEs-stimulated expression levels of receptor of AGE (RAGE), IL-6 and ICAM-1 and the phosphorylation of p38, ERK and p65 were attenuated by 6-shogaol. These results suggested that 6-shogaol inhibits AGEs-induced inflammatory responses by regulating oxidative and anti-oxidative activities and may have protective effects on periodontitis with DM.","ja":"Advanced glycation end-products (AGEs) cause diabetes mellitus (DM) complications and accumulate more highly in periodontal tissues of patients with periodontitis and DM. AGEs aggravate periodontitis with DM by increasing the expression of inflammation-related factors in periodontal tissues. 6-Shogaol, a major compound in ginger, has anti-inflammatory and anti-oxidative activities. However, the influence of shogaol on DM-associated periodontitis is not well known. In this study, the effects of 6-shogaol on AGEs-induced oxidative and anti-oxidative responses, and IL-6 and ICAM-1 expression in human gingival fibroblasts (HGFs) were investigated. When HGFs were cultured with 6-shogaol and AGEs, the activities of reactive oxygen species (ROS) and antioxidant enzymes (heme oxygenase-1 [HO-1] and NAD(P)H quinone dehydrogenase 1 [NQO1]), and IL-6 and ICAM-1 expressions were investigated. RAGE expression and phosphorylation of MAPKs and NF-κB were examined by western blotting. 6-Shogaol significantly inhibited AGEs-induced ROS activity, and increased HO-1 and NQO1 levels compared with the AGEs-treated cells. The AGEs-stimulated expression levels of receptor of AGE (RAGE), IL-6 and ICAM-1 and the phosphorylation of p38, ERK and p65 were attenuated by 6-shogaol. These results suggested that 6-shogaol inhibits AGEs-induced inflammatory responses by regulating oxidative and anti-oxidative activities and may have protective effects on periodontitis with DM."},"publication_date":"2019-10-15","publication_name":{"en":"Molecules","ja":"Molecules"},"volume":"Vol.24","number":"No.20","starting_page":"e3705","ending_page":"e3705","languages":["eng"],"referee":true,"identifiers":{"doi":["10.3390/molecules24203705"],"issn":["1420-3049"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/30735798","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=356271","label":"url"}],"paper_title":{"en":"Advanced glycation end-product 2 and Porphyromonas gingivalis lipopolysaccharide increase sclerostin expression in mouse osteocyte-like cells","ja":"Advanced glycation end-product 2 and Porphyromonas gingivalis lipopolysaccharide increase sclerostin expression in mouse osteocyte-like cells"},"authors":{"en":[{"name":"Sakamoto Eijiro"},{"name":"Kido Jun-ichi"},{"name":"Takagi Ryosuke"},{"name":"Inagaki Yuji"},{"name":"Naruishi Koji"},{"name":"Nagata Toshihiko"},{"name":"Yumoto Hiromichi"}],"ja":[{"name":"坂本 英次郎"},{"name":"木戸 淳一"},{"name":"高木 亮輔"},{"name":"稲垣 裕司"},{"name":"成石 浩司"},{"name":"永田 俊彦"},{"name":"湯本 浩通"}]},"description":{"en":"Sclerostin is a secreted glycoprotein that is mainly expressed in osteocytes, exerts negative effects on bone formation, and is present at elevated levels in diabetes mellitus (DM). Periodontitis is an infectious disease caused by periodontopathic bacteria, a complication of DM, and sometimes associated with severe inflammation and alveolar bone resorption. Advanced glycation end-products (AGEs) are a major pathogen in DM complications and adversely influence periodontitis in DM patients. In the present study, the effects of AGE2 and Porphyromonas gingivalis lipopolysaccharide (P-LPS) on the expression of sclerostin in mouse osteocyte-like cells (MLO-Y4-A2 cells) and its function in osteoblast differentiation were investigated. AGE2 and P-LPS up-regulated the expressions of receptor of AGE (RAGE) and Toll-like receptor 2 (TLR2), respectively, and significantly up-regulated that of sclerostin and interleukin 6 (IL-6) in osteocytes. Sclerostin, RAGE and TLR2 levels were synergistically increased by AGE2 and P-LPS. The siRNAs of RAGE and TLR2 significantly inhibited AGE2- and P-LPS-induced sclerostin expression. AGE2 up-regulated sclerostin expression in osteocyte-like cells via the RAGE, ERK and JNK, and NF-κB signal pathways. On the other hand, P-LPS elevated sclerostin levels via the TLR2, JNK and p38, and NF-κB signal pathways. When osteocytes pre-treated with AGE2 and P-LPS and osteoblastic cells (MC3T3-E1) were co-cultured in the medium with a sclerostin-neutralizing antibody, AGE2- and P-LPS-induced decreases in alkaline phosphatase activity and Runx2 expression in osteoblastic cells were significantly inhibited by the sclerostin-neutralizing antibody. These results suggest that AGE2 and P-LPS influence bone metabolism and inflammation through the regulation of sclerostin expression, and may aggravate periodontitis with DM.","ja":"Sclerostin is a secreted glycoprotein that is mainly expressed in osteocytes, exerts negative effects on bone formation, and is present at elevated levels in diabetes mellitus (DM). Periodontitis is an infectious disease caused by periodontopathic bacteria, a complication of DM, and sometimes associated with severe inflammation and alveolar bone resorption. Advanced glycation end-products (AGEs) are a major pathogen in DM complications and adversely influence periodontitis in DM patients. In the present study, the effects of AGE2 and Porphyromonas gingivalis lipopolysaccharide (P-LPS) on the expression of sclerostin in mouse osteocyte-like cells (MLO-Y4-A2 cells) and its function in osteoblast differentiation were investigated. AGE2 and P-LPS up-regulated the expressions of receptor of AGE (RAGE) and Toll-like receptor 2 (TLR2), respectively, and significantly up-regulated that of sclerostin and interleukin 6 (IL-6) in osteocytes. Sclerostin, RAGE and TLR2 levels were synergistically increased by AGE2 and P-LPS. The siRNAs of RAGE and TLR2 significantly inhibited AGE2- and P-LPS-induced sclerostin expression. AGE2 up-regulated sclerostin expression in osteocyte-like cells via the RAGE, ERK and JNK, and NF-κB signal pathways. On the other hand, P-LPS elevated sclerostin levels via the TLR2, JNK and p38, and NF-κB signal pathways. When osteocytes pre-treated with AGE2 and P-LPS and osteoblastic cells (MC3T3-E1) were co-cultured in the medium with a sclerostin-neutralizing antibody, AGE2- and P-LPS-induced decreases in alkaline phosphatase activity and Runx2 expression in osteoblastic cells were significantly inhibited by the sclerostin-neutralizing antibody. These results suggest that AGE2 and P-LPS influence bone metabolism and inflammation through the regulation of sclerostin expression, and may aggravate periodontitis with DM."},"publication_date":"2019-05","publication_name":{"en":"Bone","ja":"Bone"},"volume":"Vol.122","starting_page":"22","ending_page":"30","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1016/j.bone.2019.02.001"],"issn":["1873-2763"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/113370","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/30355945","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=347078","label":"url"}],"paper_title":{"en":"High Glucose-Mediated Cytokine Regulation in Gingival Fibroblasts and THP-1 Macrophage: a Possible Mechanism of Severe Periodontitis with Diabetes.","ja":"High Glucose-Mediated Cytokine Regulation in Gingival Fibroblasts and THP-1 Macrophage: a Possible Mechanism of Severe Periodontitis with Diabetes."},"authors":{"en":[{"name":"Lew Jung-Hwan"},{"name":"Naruishi Koji"},{"name":"Kajiura Yukari"},{"name":"Nishikawa Yasufumi"},{"name":"Ikuta Takahisa"},{"name":"Kido Jun-ichi"},{"name":"Nagata Toshihiko"}],"ja":[{"name":"Lew Jung-Hwan"},{"name":"成石 浩司"},{"name":"梶浦 由加里"},{"name":"Nishikawa Yasufumi"},{"name":"Ikuta Takahisa"},{"name":"木戸 淳一"},{"name":"永田 俊彦"}]},"description":{"en":"Diabetic conditions such as HG induce IL-1 and sIL-6R production from macrophages in inflammatory periodontal tissues and may exacerbate the periodontitis synergistically via MMP-1 production from HGFs.","ja":"Diabetic conditions such as HG induce IL-1 and sIL-6R production from macrophages in inflammatory periodontal tissues and may exacerbate the periodontitis synergistically via MMP-1 production from HGFs."},"publication_date":"2018-10-24","publication_name":{"en":"Cellular Physiology and Biochemistry","ja":"Cellular Physiology and Biochemistry"},"volume":"Vol.50","number":"No.3","starting_page":"973","ending_page":"986","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1159/000494481"],"issn":["1421-9778"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=347076","label":"url"}],"paper_title":{"en":"Association between oral frailty and geriatric conditions","ja":"Association between oral frailty and geriatric conditions"},"authors":{"en":[{"name":"Naruishi Koji"}],"ja":[{"name":"成石 浩司"}]},"publication_date":"2018-10","publication_name":{"en":"OBM Geriatrics","ja":"OBM Geriatrics"},"volume":"Vol.2","number":"No.4","languages":["eng"],"referee":true,"identifiers":{"doi":["10.21926/obm.geriatr.1804016"],"issn":["2638-1311"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/113617","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/29193068","label":"url"},{"@id":"https://www.scopus.com/record/display.url?eid=2-s2.0-85036593558&origin=inward","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=355533","label":"url"}],"paper_title":{"en":"Advanced glycation end-products increase IL-6 and ICAM-1 expression via RAGE, MAPK and NF-kB pathways in human gingival fibroblasts","ja":"Advanced glycation end-products increase IL-6 and ICAM-1 expression via RAGE, MAPK and NF-kB pathways in human gingival fibroblasts"},"authors":{"en":[{"name":"Nonaka Kohei"},{"name":"Kajiura Yukari"},{"name":"Bandou Mika"},{"name":"Sakamoto Eijiro"},{"name":"Inagaki Yuji"},{"name":"Lew JH"},{"name":"Naruishi Koji"},{"name":"Ikuta Takahisa"},{"name":"Yoshida Kaya"},{"name":"Kobayashi Tesuo"},{"name":"Yoshie Hiromasa"},{"name":"Nagata Toshihiko"},{"name":"Kido Jun-ichi"}],"ja":[{"name":"野中 康平"},{"name":"梶浦 由加里"},{"name":"板東 美香"},{"name":"坂本 英次郎"},{"name":"稲垣 裕司"},{"name":"Lew JH"},{"name":"成石 浩司"},{"name":"生田 貴久"},{"name":"吉田 賀弥"},{"name":"Kobayashi Tesuo"},{"name":"Yoshie Hiromasa"},{"name":"永田 俊彦"},{"name":"木戸 淳一"}]},"description":{"en":"Diabetes mellitus (DM) is a risk factor for periodontal diseases and may exacerbate the progression of the pathogenesis of periodontitis. Advanced glycation end-products (AGEs) cause DM complications relative to levels of glycemic control and larger amounts accumulate in the periodontal tissues of patients with periodontitis and DM. In the present study, we investigated the effects of AGEs on the expression of inflammation-related factors in human gingival fibroblasts (HGFs) to elucidate the impact of AGEs on DM-associated periodontitis. HGFs were cultured with or without AGEs. Cell viability was examined, and RNA and protein fractions were isolated from AGE-treated cells. The expression of interleukin (IL)-6, intercellular adhesion molecule-1 (ICAM-1), and the receptor for AGE (RAGE) was investigated using reverse transcription-polymerase chain reaction, quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, and reactive oxygen species activity was measured using a kit with 2',7'-dichlorofluorescin diacetate. Human monocytic cells (THP-1) labeled with a fluorescent reagent were co-cultured with HGFs treated with AGEs and IL-6 siRNA, and the adhesive activity of THP-1 cells to HGFs was assessed. The expression of IL-6 and ICAM-1 was examined when HGFs were pretreated with recombinant human IL-6, the siRNAs of RAGE and IL-6, and inhibitors of MAPK and NF-κB, and then cultured with and without AGEs. The phosphorylation of MAPK and NF-κB was assessed using western blotting. AGEs increased the mRNA and protein expressions of RAGE, IL-6, ICAM-1 and reactive oxygen species activity in HGFs, and promoted the adhesion of THP-1 cells to HGFs, but had no effect on cell viability until 72 hours. Recombinant human IL-6 increased ICAM-1 expression in HGFs, while the siRNAs of RAGE and IL-6 inhibited AGE-induced IL6 and ICAM1 mRNA expression, and IL-6 siRNA depressed AGE-induced THP-1 cell adhesion. AGEs increased the phosphorylation of p38 and ERK MAPKs, p65 NF-κB and IκBα, while inhibitors of p38, ERK MAPKs and NF-κB significantly decreased AGE-induced IL-6 and ICAM-1 expression. AGEs increase IL-6 and ICAM-1 expression via the RAGE, MAPK and NF-κB pathways in HGFs and may exacerbate the progression of the pathogenesis of periodontal diseases.","ja":"Diabetes mellitus (DM) is a risk factor for periodontal diseases and may exacerbate the progression of the pathogenesis of periodontitis. Advanced glycation end-products (AGEs) cause DM complications relative to levels of glycemic control and larger amounts accumulate in the periodontal tissues of patients with periodontitis and DM. In the present study, we investigated the effects of AGEs on the expression of inflammation-related factors in human gingival fibroblasts (HGFs) to elucidate the impact of AGEs on DM-associated periodontitis. HGFs were cultured with or without AGEs. Cell viability was examined, and RNA and protein fractions were isolated from AGE-treated cells. The expression of interleukin (IL)-6, intercellular adhesion molecule-1 (ICAM-1), and the receptor for AGE (RAGE) was investigated using reverse transcription-polymerase chain reaction, quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, and reactive oxygen species activity was measured using a kit with 2',7'-dichlorofluorescin diacetate. Human monocytic cells (THP-1) labeled with a fluorescent reagent were co-cultured with HGFs treated with AGEs and IL-6 siRNA, and the adhesive activity of THP-1 cells to HGFs was assessed. The expression of IL-6 and ICAM-1 was examined when HGFs were pretreated with recombinant human IL-6, the siRNAs of RAGE and IL-6, and inhibitors of MAPK and NF-κB, and then cultured with and without AGEs. The phosphorylation of MAPK and NF-κB was assessed using western blotting. AGEs increased the mRNA and protein expressions of RAGE, IL-6, ICAM-1 and reactive oxygen species activity in HGFs, and promoted the adhesion of THP-1 cells to HGFs, but had no effect on cell viability until 72 hours. Recombinant human IL-6 increased ICAM-1 expression in HGFs, while the siRNAs of RAGE and IL-6 inhibited AGE-induced IL6 and ICAM1 mRNA expression, and IL-6 siRNA depressed AGE-induced THP-1 cell adhesion. AGEs increased the phosphorylation of p38 and ERK MAPKs, p65 NF-κB and IκBα, while inhibitors of p38, ERK MAPKs and NF-κB significantly decreased AGE-induced IL-6 and ICAM-1 expression. AGEs increase IL-6 and ICAM-1 expression via the RAGE, MAPK and NF-κB pathways in HGFs and may exacerbate the progression of the pathogenesis of periodontal diseases."},"publication_date":"2018-06","publication_name":{"en":"Journal of Periodontal Research","ja":"Journal of Periodontal Research"},"volume":"Vol.53","number":"No.3","starting_page":"334","ending_page":"344","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1111/jre.12518"],"issn":["1600-0765"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/29842897","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=339706","label":"url"}],"paper_title":{"en":"Clinical effects of low body mass index on geriatric status in elderly patients.","ja":"Clinical effects of low body mass index on geriatric status in elderly patients."},"authors":{"en":[{"name":"Naruishi Koji"},{"name":"Yumoto Hiromichi"},{"name":"Kido Jun-ichi"}],"ja":[{"name":"成石 浩司"},{"name":"湯本 浩通"},{"name":"木戸 淳一"}]},"description":{"en":"Low BMI might be a useful parameter to evaluate the geriatric status, and the viewpoint would contribute to decide the care plan for the good end-of-life of elderly.","ja":"). Significant associations of low BMI to several geriatric factors such as loss of posterior occlusion, cognitive impairment were observed in both male and female. FIM scores in above cut-off point group were significantly higher than in below cut-off point group in female (FIM gain, P = 0.0005; FIM efficiency, P = 0.0025, Mann-Whitney U test). On the other hand, there were no significant differences between low and above BMI cut-off point in FIM scores of male patients."},"publication_date":"2018-05-26","publication_name":{"en":"Experimental Gerontology","ja":"Experimental Gerontology"},"volume":"Vol.110","starting_page":"86","ending_page":"91","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1016/j.exger.2018.05.017"],"issn":["1873-6815"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/29388021","label":"url"},{"@id":"https://www.scopus.com/record/display.url?eid=2-s2.0-85045039612&origin=inward","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=336184","label":"url"}],"paper_title":{"en":"Relationship of aspiration pneumonia to cognitive impairment and oral condition: a cross-sectional study.","ja":"Relationship of aspiration pneumonia to cognitive impairment and oral condition: a cross-sectional study."},"authors":{"en":[{"name":"Naruishi Koji"},{"name":"Nishikawa Yasufumi"},{"name":"Kido Jun-ichi"},{"name":"Fukunaga Akihiro"},{"name":"Nagata Toshihiko"}],"ja":[{"name":"成石 浩司"},{"name":"西川 泰史"},{"name":"木戸 淳一"},{"name":"Fukunaga Akihiro"},{"name":"永田 俊彦"}]},"description":{"en":"Early and simple evaluation of the oral condition and cognitive function can predict the risk of aspiration pneumonia.","ja":"Early and simple evaluation of the oral condition and cognitive function can predict the risk of aspiration pneumonia."},"publication_date":"2018-01","publication_name":{"en":"Clinical Oral Investigations","ja":"Clinical Oral Investigations"},"volume":"Vol.22","number":"No.7","starting_page":"2575","ending_page":"2580","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1007/s00784-018-2356-7"],"issn":["1436-3771"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/112503","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/28906038","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=334851","label":"url"}],"paper_title":{"en":"b-carotene suppresses Porphyromonas gingivalis lipopolysaccharide-mediated cytokine production in THP-1 monocytes cultured with high glucose condition.","ja":"b-carotene suppresses Porphyromonas gingivalis lipopolysaccharide-mediated cytokine production in THP-1 monocytes cultured with high glucose condition."},"authors":{"en":[{"name":"Kajiura Yukari"},{"name":"Nishikawa Yasufumi"},{"name":"Lew Jung Hwan"},{"name":"Kido Jun-ichi"},{"name":"Nagata Toshihiko"},{"name":"Naruishi Koji"}],"ja":[{"name":"梶浦 由加里"},{"name":"西川 泰史"},{"name":"LEW JUNG HWAN"},{"name":"木戸 淳一"},{"name":"永田 俊彦"},{"name":"成石 浩司"}]},"description":{"en":"Periodontitis is associated with development of diabetes mellitus. Although lipopolysaccharide (LPS) of Porphyromonas gingivalis (Pg), a major pathogen of periodontitis, may lead the progression of diabetes complications, the precise mechanisms are unclear. We, therefore, investigated the effects of β-carotene on production of Pg LPS-induced inflammatory cytokines in human monocytes cultured high glucose (HG) condition. THP-1 cells were cultured under 5.5 mM or 25 mM glucose conditions, and cells were stimulated with Pg LPS. To investigate the productivity of TNF-α, IL-6, and MCP-1, cell supernatants were collected for ELISA. To examine the effects of NF-kB signals on cytokine production, Bay11-7082 was used. HG enhanced Pg LPS-induced production of TNF-α, IL-6, and MCP-1 via NF-kB signals in THP-1. β-carotene suppressed the enhancement of the Pg LPS-induced cytokine production in THP-1 via NF-κB inactivation. Our results suggest that β-carotene might be a potential anti-inflammatory nutrient for circulating Pg LPS-mediated cytokine production in diabetic patients with periodontitis.","ja":"Periodontitis is associated with development of diabetes mellitus. Although lipopolysaccharide (LPS) of Porphyromonas gingivalis (Pg), a major pathogen of periodontitis, may lead the progression of diabetes complications, the precise mechanisms are unclear. We, therefore, investigated the effects of β-carotene on production of Pg LPS-induced inflammatory cytokines in human monocytes cultured high glucose (HG) condition. THP-1 cells were cultured under 5.5 mM or 25 mM glucose conditions, and cells were stimulated with Pg LPS. To investigate the productivity of TNF-α, IL-6, and MCP-1, cell supernatants were collected for ELISA. To examine the effects of NF-kB signals on cytokine production, Bay11-7082 was used. HG enhanced Pg LPS-induced production of TNF-α, IL-6, and MCP-1 via NF-kB signals in THP-1. β-carotene suppressed the enhancement of the Pg LPS-induced cytokine production in THP-1 via NF-κB inactivation. Our results suggest that β-carotene might be a potential anti-inflammatory nutrient for circulating Pg LPS-mediated cytokine production in diabetic patients with periodontitis."},"publication_date":"2018-01-14","publication_name":{"en":"Cell Biology International","ja":"Cell Biology International"},"volume":"Vol.42","number":"No.1","starting_page":"105","ending_page":"111","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1002/cbin.10873"],"issn":["1095-8355"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/28391586","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=326804","label":"url"}],"paper_title":{"en":"Swallowing impairment is a significant factor for predicting life prognosis of elderly at the end of life.","ja":"Swallowing impairment is a significant factor for predicting life prognosis of elderly at the end of life."},"authors":{"en":[{"name":"Naruishi Koji"},{"name":"Nishikawa Yasufumi"}],"ja":[{"name":"成石 浩司"},{"name":"Nishikawa Yasufumi"}]},"description":{"en":"For judgement of life prognosis, the condition of being frail such as impaired swallowing function might be a useful factor, and the viewpoint would contribute to decide the treatment plan for the good end-of-life care of elderly.","ja":"A total of 320 elderly patients was enrolled (male/female 151/169; averaged age: male 84.7 ± 5.9 year, female 86.8 ± 6.3 year) and retrospective analyses were performed. The elderly patients were classified as either: (1) with or without past illness of aspiration pneumonia; (2) with or without incidence of cerebrovascular disorder; (3) impaired or normal cognitive function; (4) impaired or normal swallowing function, and performed Kaplan-Meier survival analysis. Swallowing function was examined using video endoscopic (VE) evaluation method. The Kaplan-Meier analysis of the number of days from implementation of VE test (day 0) to death was evaluated with the log-rank Mantel-Cox test. The maximum follow-up time recorded was 180 days."},"publication_date":"2018-01-08","publication_name":{"en":"Aging Clinical and Experimental Research","ja":"Aging Clinical and Experimental Research"},"volume":"Vol.30","number":"No.1","starting_page":"77","ending_page":"80","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1007/s40520-017-0756-1"],"issn":["1720-8319"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/29107933","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=334850","label":"url"}],"paper_title":{"en":"Local administration of calcitonin inhibits alveolar bone loss in an experimental periodontitis in rats.","ja":"Local administration of calcitonin inhibits alveolar bone loss in an experimental periodontitis in rats."},"authors":{"en":[{"name":"Wada -Mihara Chie"},{"name":"Seto Hiroyuki"},{"name":"Ohba Hirofumi"},{"name":"Tokunaga Kaku"},{"name":"Kido Jun-ichi"},{"name":"Nagata Toshihiko"},{"name":"Naruishi Koji"}],"ja":[{"name":"美原(和田) 智恵"},{"name":"瀬戸 浩行"},{"name":"Ohba Hirofumi"},{"name":"Tokunaga Kaku"},{"name":"木戸 淳一"},{"name":"永田 俊彦"},{"name":"成石 浩司"}]},"description":{"en":"Calcitonin (CTN), a calcium regulatory hormone, promotes calcium diuresis from the kidney and suppresses bone resorption. The objective of this study was to evaluate whether the topical and intermittent application of CTN inhibits alveolar bone resorption using ligature-induced experimental periodontitis in rats. Experimental periodontitis was induced by placing a nylon ligature around maxillary molars of 8-week-old male Wistar rats for 20 days. Thirty-two rats were divided into four groups: basal sham control group, periodontitis group, periodontitis plus 0.2 U CTN (low dose), and periodontitis plus 1.0 U CTN (high dose) group. To investigate the effects of CTN on alveolar bone resorption, CTN was topically injected into the palatal gingivae every 2 days after ligature removal (day 0). Micro-computed tomography (CT) analysis was performed for linear parameter assessment of alveolar bone on day 5 and day 14. Periodontal tissues were examined histo-pathologically to assess the differences among the study groups. Micro-CT images showed that alveolar bone resorption was induced statistically around the molar of ligatured rats on day 5 and day 14. The amount of bone resorption was more severe on day 14 than that on day 5. On day 5, only high-dose CTN treatment significantly suppressed bone resorption. In addition, both doses of CTN significantly suppressed bone resorption on day 14. Histological examination clarified that there were fewer TRAP-positive cells in the CTN treatment groups than in the periodontitis group on day 5. Local administration of CTN decreased alveolar bone resorption by regulating osteoclast activation in rats with periodontitis.","ja":"Calcitonin (CTN), a calcium regulatory hormone, promotes calcium diuresis from the kidney and suppresses bone resorption. The objective of this study was to evaluate whether the topical and intermittent application of CTN inhibits alveolar bone resorption using ligature-induced experimental periodontitis in rats. Experimental periodontitis was induced by placing a nylon ligature around maxillary molars of 8-week-old male Wistar rats for 20 days. Thirty-two rats were divided into four groups: basal sham control group, periodontitis group, periodontitis plus 0.2 U CTN (low dose), and periodontitis plus 1.0 U CTN (high dose) group. To investigate the effects of CTN on alveolar bone resorption, CTN was topically injected into the palatal gingivae every 2 days after ligature removal (day 0). Micro-computed tomography (CT) analysis was performed for linear parameter assessment of alveolar bone on day 5 and day 14. Periodontal tissues were examined histo-pathologically to assess the differences among the study groups. Micro-CT images showed that alveolar bone resorption was induced statistically around the molar of ligatured rats on day 5 and day 14. The amount of bone resorption was more severe on day 14 than that on day 5. On day 5, only high-dose CTN treatment significantly suppressed bone resorption. In addition, both doses of CTN significantly suppressed bone resorption on day 14. Histological examination clarified that there were fewer TRAP-positive cells in the CTN treatment groups than in the periodontitis group on day 5. Local administration of CTN decreased alveolar bone resorption by regulating osteoclast activation in rats with periodontitis."},"publication_date":"2018-01-03","publication_name":{"en":"Biomedicine & Pharmacotherapy","ja":"Biomedicine & Pharmacotherapy"},"volume":"Vol.97","number":"No.1","starting_page":"765","ending_page":"770","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1016/j.biopha.2017.10.165"],"issn":["1950-6007"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/28771806","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=327751","label":"url"}],"paper_title":{"en":"Advanced glycation end-products and Porphyromonas gingivalis lipopolysaccharide increase calprotectin expression in human gingival epithelial cells.","ja":"Advanced glycation end-products and Porphyromonas gingivalis lipopolysaccharide increase calprotectin expression in human gingival epithelial cells."},"authors":{"en":[{"name":"Hiroshima Yuka"},{"name":"Sakamoto Eijiro"},{"name":"Yoshida Kaya"},{"name":"Abe Kaori"},{"name":"Naruishi Koji"},{"name":"Yamamoto Takenori"},{"name":"Shinohara Yasuo"},{"name":"Kido Jun-ichi"},{"name":"Geczy L Carolyn"}],"ja":[{"name":"廣島 佑香"},{"name":"坂本 英次郎"},{"name":"吉田 賀弥"},{"name":"阿部 佳織"},{"name":"成石 浩司"},{"name":"山本 武範"},{"name":"篠原 康雄"},{"name":"木戸 淳一"},{"name":"Geczy L Carolyn"}]},"description":{"en":"Accumulation of advanced glycation end-products (AGEs) in periodontal tissues of patients with diabetes mellitus aggravates periodontitis, but the mechanisms are unknown. Calprotectin, a heterocomplex of S100A8 and S100A9 proteins, is a constitutive cytoplasmic component of healthy gingival epithelial cells. This study aimed at investigating the effects of AGE and Porphyromonas gingivalis lipopolysaccharide (PgLPS) on calprotectin expression in the human gingival epithelial cell line OBA-9. AGE and PgLPS increased the expression of S100A8 and S100A9 mRNAs, and AGE + PgLPS co-stimulation amplified their expression in OBA-9 cells. A higher concentration of calprotectin in cell lysates was also induced by stimulation with AGE and/or PgLPS. S100A8 was mainly translocated from the nucleus to the cytoplasm by AGE stimulation, while cytoplasmic localization of S100A9 was not altered following stimulation with AGE and/or PgLPS. Calprotectin was found in the cytoplasm of BSA-treated cells, but cytoplasmic and nuclear localization was observed following stimulation with AGE and/or PgLPS. AGE-induced S100A8, and S100A9 mRNA expression was partially suppressed by RAGE-specific siRNA. In contrast, PgLPS-induced S100A8 and S100A9 mRNA expression was strongly suppressed by TLR2-specific siRNA. Furthermore, the inhibition of p38, JNK MAPK, and NF-κB attenuated AGE- and PgLPS-induced S100A8 and S100A9 mRNA expression. Taken together, these results demonstrate that AGE acts in synergy with PgLPS to stimulate RAGE and TLR2 expression and activate p38, JNK MAPK, and NF-κB signaling pathways, resulting in increased activation of calprotectin (S100A8/S100A9) in human gingival epithelial cells. Our results suggest that calprotectin may be involved in the pathogenesis of diabetic periodontitis. This article is protected by copyright. All rights reserved.","ja":"Accumulation of advanced glycation end-products (AGEs) in periodontal tissues of patients with diabetes mellitus aggravates periodontitis, but the mechanisms are unknown. Calprotectin, a heterocomplex of S100A8 and S100A9 proteins, is a constitutive cytoplasmic component of healthy gingival epithelial cells. This study aimed at investigating the effects of AGE and Porphyromonas gingivalis lipopolysaccharide (PgLPS) on calprotectin expression in the human gingival epithelial cell line OBA-9. AGE and PgLPS increased the expression of S100A8 and S100A9 mRNAs, and AGE + PgLPS co-stimulation amplified their expression in OBA-9 cells. A higher concentration of calprotectin in cell lysates was also induced by stimulation with AGE and/or PgLPS. S100A8 was mainly translocated from the nucleus to the cytoplasm by AGE stimulation, while cytoplasmic localization of S100A9 was not altered following stimulation with AGE and/or PgLPS. Calprotectin was found in the cytoplasm of BSA-treated cells, but cytoplasmic and nuclear localization was observed following stimulation with AGE and/or PgLPS. AGE-induced S100A8, and S100A9 mRNA expression was partially suppressed by RAGE-specific siRNA. In contrast, PgLPS-induced S100A8 and S100A9 mRNA expression was strongly suppressed by TLR2-specific siRNA. Furthermore, the inhibition of p38, JNK MAPK, and NF-κB attenuated AGE- and PgLPS-induced S100A8 and S100A9 mRNA expression. Taken together, these results demonstrate that AGE acts in synergy with PgLPS to stimulate RAGE and TLR2 expression and activate p38, JNK MAPK, and NF-κB signaling pathways, resulting in increased activation of calprotectin (S100A8/S100A9) in human gingival epithelial cells. Our results suggest that calprotectin may be involved in the pathogenesis of diabetic periodontitis. This article is protected by copyright. All rights reserved."},"publication_date":"2018","publication_name":{"en":"Journal of Cellular Biochemistry","ja":"Journal of Cellular Biochemistry"},"volume":"Vol.119","number":"No.2","starting_page":"1591","ending_page":"1603","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1002/jcb.26319"],"issn":["1097-4644"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/28875823","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=334852","label":"url"}],"paper_title":{"en":"Useful Immunochromatographic Assay of Calprotectin in Gingival Crevicular Fluid for Diagnosis of Diseased Sites in Patients with Periodontal Diseases.","ja":"Useful Immunochromatographic Assay of Calprotectin in Gingival Crevicular Fluid for Diagnosis of Diseased Sites in Patients with Periodontal Diseases."},"authors":{"en":[{"name":"Kido Jun-ichi"},{"name":"Murakami Shinya"},{"name":"Kitamura Masahiro"},{"name":"Yanagita Manabu"},{"name":"Tabeta Koichi"},{"name":"Yamazaki Kazuhisa"},{"name":"Yoshie Hiromasa"},{"name":"Watanabe Hisashi"},{"name":"Izumi Yuichi"},{"name":"Suda Reiko"},{"name":"Yamamoto Matsuo"},{"name":"Shiba Hideki"},{"name":"Fujita Tsuyoshi"},{"name":"Kurihara Hidemi"},{"name":"Mizuno Mitsuharu"},{"name":"Mishima Akihiro"},{"name":"Kawahara Nobumasa"},{"name":"Hashimoto Kazuhiro"},{"name":"Naruishi Koji"},{"name":"Nagata Toshihiko"}],"ja":[{"name":"木戸 淳一"},{"name":"Murakami Shinya"},{"name":"Kitamura Masahiro"},{"name":"Yanagita Manabu"},{"name":"Tabeta Koichi"},{"name":"Yamazaki Kazuhisa"},{"name":"Yoshie Hiromasa"},{"name":"Watanabe Hisashi"},{"name":"Izumi Yuichi"},{"name":"Suda Reiko"},{"name":"Yamamoto Matsuo"},{"name":"Shiba Hideki"},{"name":"Fujita Tsuyoshi"},{"name":"Kurihara Hidemi"},{"name":"Mizuno Mitsuharu"},{"name":"Mishima Akihiro"},{"name":"Kawahara Nobumasa"},{"name":"Hashimoto Kazuhiro"},{"name":"成石 浩司"},{"name":"永田 俊彦"}]},"description":{"en":"Determination of GCF calprotectin using a novel IC chip system is useful for diagnosis of periodontal diseases.","ja":"Determination of GCF calprotectin using a novel IC chip system is useful for diagnosis of periodontal diseases."},"publication_date":"2017-09-06","publication_name":{"en":"Journal of Periodontology","ja":"Journal of Periodontology"},"starting_page":"1","ending_page":"19","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1902/jop.2017.170206"],"issn":["1943-3670"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/115109","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=332586","label":"url"}],"paper_title":{"en":"Association between Oral Conditions and Returning Home after Discharge in Elderly Patients","ja":"Association between Oral Conditions and Returning Home after Discharge in Elderly Patients"},"authors":{"en":[{"name":"Naruishi Koji"}],"ja":[{"name":"成石 浩司"}]},"publication_date":"2017-08-25","publication_name":{"en":"Geriatrics","ja":"Geriatrics"},"volume":"Vol.2","number":"No.3","starting_page":"28","ending_page":"28","languages":["eng"],"referee":true,"identifiers":{"doi":["10.3390/geriatrics2030028"],"issn":["2308-3417"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/27810997","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=326803","label":"url"}],"paper_title":{"en":"Clinical Significance of GCF sIL-6R and Calprotectin to Evaluate the Periodontal Inflammation.","ja":"Clinical Significance of GCF sIL-6R and Calprotectin to Evaluate the Periodontal Inflammation."},"authors":{"en":[{"name":"Kajiura Yukari"},{"name":"Lew Jung Hwan"},{"name":"Ikuta Takahisa"},{"name":"Nishikawa Yasufumi"},{"name":"Kido Jun-ichi"},{"name":"Nagata Toshihiko"},{"name":"Naruishi Koji"}],"ja":[{"name":"梶浦 由加里"},{"name":"Lew Jung Hwan"},{"name":"Ikuta Takahisa"},{"name":"Nishikawa Yasufumi"},{"name":"木戸 淳一"},{"name":"永田 俊彦"},{"name":"成石 浩司"}]},"description":{"en":"Both GCF sIL-6R and calprotectin levels are significant bio-markers to evaluate the periodontal inflammation.","ja":"Both GCF sIL-6R and calprotectin levels are significant bio-markers to evaluate the periodontal inflammation."},"publication_date":"2017-06-03","publication_name":{"en":"Annals of Clinical Biochemistry","ja":"Annals of Clinical Biochemistry"},"volume":"Vol.54","number":"No.6","starting_page":"664","ending_page":"670","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1177/0004563216680232"],"issn":["1758-1001"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/111059","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=330877","label":"url"}],"paper_title":{"en":"Post clinical clerkship-OSCEを見据えた歯周病科での模擬OSCEの概要","ja":"Post clinical clerkship-OSCEを見据えた歯周病科での模擬OSCEの概要"},"authors":{"en":[{"name":"Wada -Mihara Chie"},{"name":"Naruishi Koji"},{"name":"Bandou Mika"},{"name":"Nishikawa Yasufumi"},{"name":"Jung-Hwan Lew"},{"name":"Sakamoto Eijiro"},{"name":"Ikuta Takahisa"},{"name":"Kono Kaoru"},{"name":"Kajiura Yukari"},{"name":"Hashimoto Mari"},{"name":"Nakajima Yukiko"},{"name":"Inagaki Yuji"},{"name":"Ninomiya Masami"},{"name":"Kido Jun-ichi"},{"name":"Nagata Toshihiko"}],"ja":[{"name":"美原(和田) 智恵"},{"name":"成石 浩司"},{"name":"板東 美香"},{"name":"西川 泰史"},{"name":"Jung-Hwan Lew"},{"name":"坂本 英次郎"},{"name":"生田 貴久"},{"name":"河野 薫"},{"name":"梶浦 由加里"},{"name":"橋本 万里"},{"name":"中島 由紀子"},{"name":"稲垣 裕司"},{"name":"二宮 雅美"},{"name":"木戸 淳一"},{"name":"永田 俊彦"}]},"publication_date":"2017-02","publication_name":{"en":"Journal of Oral Health and Biosciences","ja":"Journal of Oral Health and Biosciences"},"volume":"Vol.29","number":"No.2","starting_page":"49","ending_page":"53","languages":["jpn"],"referee":true,"identifiers":{"doi":["10.20738/johb.29.2_49"],"issn":["2188-7888"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/111725","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/27925202","label":"url"},{"@id":"https://www.scopus.com/record/display.url?eid=2-s2.0-85008440585&origin=inward","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=326016","label":"url"}],"paper_title":{"en":"Calprotectin Induces IL-6 and MCP-1 Production via Toll-Like Receptor 4 Signaling in Human Gingival Fibroblasts.","ja":"Calprotectin Induces IL-6 and MCP-1 Production via Toll-Like Receptor 4 Signaling in Human Gingival Fibroblasts."},"authors":{"en":[{"name":"Nishikawa Yasufumi"},{"name":"Kajiura Yukari"},{"name":"Lew Jung Hwan"},{"name":"Kido Jun-ichi"},{"name":"Nagata Toshihiko"},{"name":"Naruishi Koji"}],"ja":[{"name":"西川 泰史"},{"name":"梶浦 由加里"},{"name":"Lew Jung Hwan"},{"name":"木戸 淳一"},{"name":"永田 俊彦"},{"name":"成石 浩司"}]},"description":{"en":"Calprotectin, a heterodimer of S100A8 and S100A9 molecules, is associated with inflammatory diseases such as inflammatory bowel disease. We have reported that calprotectin levels in gingival crevicular fluids of periodontitis patients are significantly higher than in healthy subjects. However, the functions of calprotectin in pathophysiology of periodontitis are still unknown. The aim of this study is to investigate the effects of calprotectin on the productivity of inflammatory cytokines in human gingival fibroblasts (HGFs). The HGFs cell line CRL-2014® (ATCC) were cultured, and total RNAs were collected to examine the expression of TLR2/4 and RAGE mRNA using RT-PCR. After the cells were treated with S100A8, S100A9, and calprotectin, supernatants were collected and the levels of IL-6 and MCP-1 were measured using ELISA methods. To examine the intracellular signals involved in calprotectin-induced cytokine production, several chemical inhibitors were used. Furthermore, after the siRNA-mediated TLR4 down-regulated cells were treated with S100A8, S100A9, and calprotectin, the levels of IL-6 and MCP-1 were also measured. HGFs showed greater expression of TLR4 mRNA, but not TLR2 and RAGE mRNA compared with human oral epithelial cells. Calprotectin increased significantly the production of MCP-1 and IL-6 in HGFs, and the cytokine productions were significantly suppressed in the cells treated with MAPKs, NF-B, and TLR4 inhibitors. Furthermore, calprotectin-mediated MCP-1 and IL-6 production were significantly suppressed in TLR4 down-regulated cells. Taken together, calprotectin induces IL-6 and MCP-1 production in HGFs via TLR4 signaling that involves MAPK and NF-B, resulting in the progression of periodontitis. J. Cell. Physiol. 232: 1862-1871, 2017. © 2016 Wiley Periodicals, Inc.","ja":"Calprotectin, a heterodimer of S100A8 and S100A9 molecules, is associated with inflammatory diseases such as inflammatory bowel disease. We have reported that calprotectin levels in gingival crevicular fluids of periodontitis patients are significantly higher than in healthy subjects. However, the functions of calprotectin in pathophysiology of periodontitis are still unknown. The aim of this study is to investigate the effects of calprotectin on the productivity of inflammatory cytokines in human gingival fibroblasts (HGFs). The HGFs cell line CRL-2014® (ATCC) were cultured, and total RNAs were collected to examine the expression of TLR2/4 and RAGE mRNA using RT-PCR. After the cells were treated with S100A8, S100A9, and calprotectin, supernatants were collected and the levels of IL-6 and MCP-1 were measured using ELISA methods. To examine the intracellular signals involved in calprotectin-induced cytokine production, several chemical inhibitors were used. Furthermore, after the siRNA-mediated TLR4 down-regulated cells were treated with S100A8, S100A9, and calprotectin, the levels of IL-6 and MCP-1 were also measured. HGFs showed greater expression of TLR4 mRNA, but not TLR2 and RAGE mRNA compared with human oral epithelial cells. Calprotectin increased significantly the production of MCP-1 and IL-6 in HGFs, and the cytokine productions were significantly suppressed in the cells treated with MAPKs, NF-B, and TLR4 inhibitors. Furthermore, calprotectin-mediated MCP-1 and IL-6 production were significantly suppressed in TLR4 down-regulated cells. Taken together, calprotectin induces IL-6 and MCP-1 production in HGFs via TLR4 signaling that involves MAPK and NF-B, resulting in the progression of periodontitis. J. Cell. Physiol. 232: 1862-1871, 2017. © 2016 Wiley Periodicals, Inc."},"publication_date":"2017-01-06","publication_name":{"en":"Journal of Cellular Physiology","ja":"Journal of Cellular Physiology"},"volume":"Vol.232","number":"No.7","starting_page":"1862","ending_page":"1871","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1002/jcp.25724"],"issn":["1097-4652"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/115061","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=326805","label":"url"}],"paper_title":{"en":"Association between Periodontal Condition and Kidney Dysfunction in Japanese Adults: A Cross-Sectional Study","ja":"Association between Periodontal Condition and Kidney Dysfunction in Japanese Adults: A Cross-Sectional Study"},"authors":{"en":[{"name":"Naruishi Koji"},{"name":"Oishi Keiji"},{"name":"Inagaki Yuuji"},{"name":"Horibe Masumi"},{"name":"Bandou Mika"},{"name":"Ninomiya Masami"},{"name":"Kawahara K"},{"name":"Minakuchi J"},{"name":"Kawashima S"},{"name":"Shima K"},{"name":"Kido Jun-ichi"},{"name":"Nagata Toshihiko"}],"ja":[{"name":"成石 浩司"},{"name":"大石 慶二"},{"name":"Inagaki Yuuji"},{"name":"堀部 ますみ"},{"name":"板東 美香"},{"name":"二宮 雅美"},{"name":"Kawahara K"},{"name":"Minakuchi J"},{"name":"Kawashima S"},{"name":"Shima K"},{"name":"木戸 淳一"},{"name":"永田 俊彦"}]},"publication_date":"2016-06-10","publication_name":{"en":"Clinical and Experimental Dental Research","ja":"Clinical and Experimental Dental Research"},"volume":"Vol.2","number":"No.2","starting_page":"1","ending_page":"8","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1002/cre2.39"],"issn":["2057-4347"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/26251312","label":"url"},{"@id":"https://www.scopus.com/record/display.url?eid=2-s2.0-84938650739&origin=inward","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=298711","label":"url"}],"paper_title":{"en":"Effects of new over-the-counter periodontal ointment-containing applicator with single-tuft brush on cytokine levels in gingival crevicular fluid during supportive periodontal therapy phase: a randomized double-blind clinical trial.","ja":"Effects of new over-the-counter periodontal ointment-containing applicator with single-tuft brush on cytokine levels in gingival crevicular fluid during supportive periodontal therapy phase: a randomized double-blind clinical trial."},"authors":{"en":[{"name":"Takeuchi-Hatanaka K"},{"name":"Yasuda T"},{"name":"Naruishi Koji"},{"name":"Katsuragi-Fuke K"},{"name":"Inubushi J"},{"name":"Ootsuki H"},{"name":"Maeda H"},{"name":"Takashiba S"}],"ja":[{"name":"Takeuchi-Hatanaka K"},{"name":"Yasuda T"},{"name":"成石 浩司"},{"name":"Katsuragi-Fuke K"},{"name":"Inubushi J"},{"name":"Ootsuki H"},{"name":"Maeda H"},{"name":"Takashiba S"}]},"description":{"en":"This OTC medication is biochemically effective for steady chronic periodontitis in the supportive periodontal therapy phase.","ja":"The levels of IL-1β, IL-6, IL-8 and TNF-α remained significantly lower in the test group compared to the placebo group. In the placebo group, when the probing pocket depth at baseline was 4 mm, IL-1β increased, particularly in the second molar tooth, and the greatest increase was seen when PPD at baseline was 5-6 mm. In the test group, IL-1β decreased markedly in cases with furcation involvement and low bleeding on probing at baseline. In both groups, IL-1β, IL-6 and TNF-α were closely correlated with each other."},"publication_date":"2016-06","publication_name":{"en":"Journal of Periodontal Research","ja":"Journal of Periodontal Research"},"volume":"Vol.51","number":"No.3","starting_page":"321","ending_page":"331","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1111/jre.12311"],"issn":["1600-0765"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"http://ci.nii.ac.jp/naid/130005086794/","label":"url"},{"@id":"https://cir.nii.ac.jp/crid/1390001205520665472/","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=297907","label":"url"}],"paper_title":{"en":"Inhibitory Effects of Cryptotanshinone on Inflammation-related Molecules in Human Gingival Fibroblasts","ja":"培養歯肉線維芽細胞におけるクリプトタンシノンによる炎症関連分子の産生抑制効果"},"authors":{"en":[{"name":"Naruishi Koji"},{"name":"Kajiura Yukari"},{"name":"西川 泰史"},{"name":"Bandou Mika"},{"name":"Kido Jun-ichi"},{"name":"Nagata Toshihiko"}],"ja":[{"name":"成石 浩司"},{"name":"梶浦 由加里"},{"name":"西川 泰史"},{"name":"板東 美香"},{"name":"木戸 淳一"},{"name":"永田 俊彦"}]},"description":{"en":"Purpose: Cryptotanshinone (CPT) isolated from the root of an Asian medicinal plant, Salvia miltiorrhiza bunge, acts as a potent anti-tumor or anti-inflammatory agent in vitro. However, the effects of CPT on the progression of periodontitis are still unknown. This study investigated the effects of CPT on the production of inflammation-related molecules in human gingival fibroblasts. Methods: Human gingival fibroblasts (HGFs), CRL-2014TM (ATCC), were used. Cytotoxicity of the cells by CPT was examined. Dead cells stained by trypan blue were counted and analyzed. Next, the levels of IL-6 and VEGF in IL-1β-treated cells with or without CPT were measured using ELISA kits. The productivity of cathepsin L in IL-1β-treated cells with or without CPT was examined using Western blotting. Results: The ratio of viable cell numbers was significantly decreased by 100 μmol/l CPT. Next, the increase of IL-6 and cathepsin L induced by IL-1β was significantly suppressed by the pretreatment of 10 μmol/l CPT in HGFs. On the other hand, productivity of VEGF induced by IL-1β was not changed by the pretreatment of 10 μmol/l CPT. Conclusion: CPT suppresses IL-1β-induced IL-6 and cathepsin L production in HGFs, and might regulate the progression of periodontitis.","ja":"目的 : 漢方薬 (生薬) タンジンの主成分であるクリプトタンシノンは, 抗がん作用や抗炎症作用をもつことが知られている. しかしながら, クリプトタンシノンが歯周組織の炎症に対してどのような影響を及ぼすのかは不明である. 本研究の目的は, 歯肉線維芽細胞を標的として, その炎症関連分子の産生に及ぼすクリプトタンシノンの影響を調べることである. 方法 : 細胞は歯肉線維芽細胞CRL-2014TM (ATCC) を用いた. クリプトタンシノンの濃度依存的な細胞障害作用の有無は, トリパンブルー染色法を用いて各濃度間における生細胞率の差を比較検討して評価した. 歯肉線維芽細胞のサイトカイン産生は, 市販のELISAキットを用いて, インターロイキン (IL) -1βで細胞を24時間刺激した後のIL-6およびVEGF産生量を測定して検討した. 一方, タンパク質分解酵素カテプシンLの産生は, IL-1βで細胞を24時間刺激した後に回収した全細胞タンパク質を用いて, ウエスタンブロット法によって検討した. なお, クリプトタンシノンはIL-1β添加の1時間前に細胞培養系に添加した. 各群における差異は, ANOVA Tukey-KramerのHSD検定によって有意差を検討した. 結果 : クリプトタンシノンは, 10μmol/lまでは歯肉線維芽細胞の生細胞率に影響は与えないが, 100μmol/lでは有意に生細胞率を低下させた. また, 歯肉線維芽細胞におけるIL-1β誘導性のIL-6産生およびカテプシンL産生は, 10μmol/lクリプトタンシノンの添加によって著明に減少した. 一方, IL-1β誘導性のVEGF産生は, クリプトタンシノンの添加によっても変化しなかった. 結論 : クリプトタンシノンは, 歯肉線維芽細胞におけるIL-1β誘導性IL-6およびカテプシンLの産生を抑制することで, 歯周組織の炎症を抑制する可能性が示唆された."},"publication_date":"2015-06","publication_name":{"en":"The Japanese Journal of Conservative Dentistry","ja":"日本歯科保存学雑誌"},"volume":"Vol.58","number":"No.3","starting_page":"212","ending_page":"218","languages":["jpn"],"referee":true,"identifiers":{"doi":["10.11471/shikahozon.58.212"],"issn":["0387-2343"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/25740558","label":"url"},{"@id":"https://www.scopus.com/record/display.url?eid=2-s2.0-84930271794&origin=inward","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=339758","label":"url"}],"paper_title":{"en":"YKL-40 level in gingival crevicular fluid from patients with periodontitis and type 2 diabetes","ja":"YKL-40 level in gingival crevicular fluid from patients with periodontitis and type 2 diabetes"},"authors":{"en":[{"name":"Kido Jun-ichi"},{"name":"Bandou Yukiko"},{"name":"Bandou Mika"},{"name":"Kajiura Yukari"},{"name":"Hiroshima Yuka"},{"name":"Inagaki Yuji"},{"name":"Murata Hiromi"},{"name":"Ikuta Takahisa"},{"name":"Kido Reiko"},{"name":"Naruishi Koji"},{"name":"Funaki Makoto"},{"name":"Nagata Toshihiko"}],"ja":[{"name":"木戸 淳一"},{"name":"坂東 由記子"},{"name":"板東 美香"},{"name":"梶浦 由加里"},{"name":"廣島 佑香"},{"name":"稲垣 裕司"},{"name":"村田 裕美"},{"name":"生田 貴久"},{"name":"木戸 玲子"},{"name":"成石 浩司"},{"name":"船木 真理"},{"name":"永田 俊彦"}]},"description":{"en":"YKL-40 is a chitin-binding glycoprotein, the level of which increases in inflammatory diseases, diabetes mellitus (DM), cardiovascular diseases, and tumors. Gingival crevicular fluid (GCF) contains many proteins and markers of periodontitis. The purpose of this study was to investigate YKL-40 level in GCF from patients with periodontitis and DM and the association between YKL-40 level and chronic periodontitis (CP) or DM. The subjects were 121 patients with DM, CP, DM and periodontitis (DM-P), and healthy subjects (H). GCF was collected using paper strips after the sites for GCF collection were clinically evaluated for probing depth (PD), gingival index (GI), and bleeding on probing (BOP). YKL-40 in GCF was identified by Western blotting, and its level was determined by ELISA. YKL-40 was contained in GCF samples from H, DM, CP, and DM-P sites, and its levels (amount and concentration) in CP and DM-P were significantly higher than those in H and DM. GCF YKL-40 level significantly correlated with PD and GI, and its level in BOP-positive sites was significantly higher than that in BOP-negative ones. GCF YKL-40 level was elevated in periodontitis, but not DM. YKL-40 in GCF may be an inflammatory marker for periodontitis.","ja":"YKL-40 is a chitin-binding glycoprotein, the level of which increases in inflammatory diseases, diabetes mellitus (DM), cardiovascular diseases, and tumors. Gingival crevicular fluid (GCF) contains many proteins and markers of periodontitis. The purpose of this study was to investigate YKL-40 level in GCF from patients with periodontitis and DM and the association between YKL-40 level and chronic periodontitis (CP) or DM. The subjects were 121 patients with DM, CP, DM and periodontitis (DM-P), and healthy subjects (H). GCF was collected using paper strips after the sites for GCF collection were clinically evaluated for probing depth (PD), gingival index (GI), and bleeding on probing (BOP). YKL-40 in GCF was identified by Western blotting, and its level was determined by ELISA. YKL-40 was contained in GCF samples from H, DM, CP, and DM-P sites, and its levels (amount and concentration) in CP and DM-P were significantly higher than those in H and DM. GCF YKL-40 level significantly correlated with PD and GI, and its level in BOP-positive sites was significantly higher than that in BOP-negative ones. GCF YKL-40 level was elevated in periodontitis, but not DM. YKL-40 in GCF may be an inflammatory marker for periodontitis."},"publication_date":"2015-04-06","publication_name":{"en":"Oral Diseases","ja":"Oral Diseases"},"volume":"Vol.21","number":"No.5","starting_page":"667","ending_page":"673","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1111/odi.12334"],"issn":["1601-0825"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://cir.nii.ac.jp/crid/1390001204416151552/","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=315232","label":"url"}],"paper_title":{"en":"肥満糖尿病ラット歯周炎の歯肉組織におけるオステオポンチンの局在","ja":"肥満糖尿病ラット歯周炎の歯肉組織におけるオステオポンチンの局在"},"authors":{"en":[{"name":"Inagaki Yuji"},{"name":"Nakajima Yukiko"},{"name":"Horibe Masumi"},{"name":"生田 貴久"},{"name":"Kajiura Yukari"},{"name":"Naruishi Koji"},{"name":"Kido Jun-ichi"},{"name":"Nagata Toshihiko"}],"ja":[{"name":"稲垣 裕司"},{"name":"中島 由紀子"},{"name":"堀部 ますみ"},{"name":"生田 貴久"},{"name":"梶浦 由加里"},{"name":"成石 浩司"},{"name":"木戸 淳一"},{"name":"永田 俊彦"}]},"publication_date":"2015","publication_name":{"en":"Journal of the Japanese Association of Periodontology","ja":"日本歯周病学会会誌"},"volume":"Vol.67","number":"No.4","starting_page":"149","ending_page":"158","languages":["jpn"],"referee":true,"identifiers":{"doi":["10.2329/perio.57.149"],"issn":["1880-408X"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/25584025","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=289597","label":"url"}],"paper_title":{"en":"Predictors of improved functional outcome in elderly inpatients after rehabilitation: a retrospective study.","ja":"Predictors of improved functional outcome in elderly inpatients after rehabilitation: a retrospective study."},"authors":{"en":[{"name":"Naruishi Koji"},{"name":"Kunita Akiko"},{"name":"Kubo Katsuyuki"},{"name":"Nagata Toshihiko"},{"name":"Takashiba Shogo"},{"name":"Adachi Seiji"}],"ja":[{"name":"成石 浩司"},{"name":"Kunita Akiko"},{"name":"Kubo Katsuyuki"},{"name":"永田 俊彦"},{"name":"Takashiba Shogo"},{"name":"Adachi Seiji"}]},"description":{"en":"Age and disorder of oral function may be significant predictors of improved functional capacity after rehabilitation for elderly inpatients.","ja":"Age and disorder of oral function may be significant predictors of improved functional capacity after rehabilitation for elderly inpatients."},"publication_date":"2014-12-05","publication_name":{"en":"Clinical Interventions in Aging","ja":"Clinical Interventions in Aging"},"volume":"Vol.9","starting_page":"2133","ending_page":"2141","languages":["eng"],"referee":true,"identifiers":{"doi":["10.2147/CIA.S73388"],"issn":["1178-1998"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/25548632","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=289596","label":"url"}],"paper_title":{"en":"Gingival overgrowth caused by vitamin C deficiency associated with metabolic syndrome and severe periodontal infection: a case report.","ja":"Gingival overgrowth caused by vitamin C deficiency associated with metabolic syndrome and severe periodontal infection: a case report."},"authors":{"en":[{"name":"Omori Kazuhiro"},{"name":"Hanayama Yoshihisa"},{"name":"Naruishi Koji"},{"name":"Akiyama Kentaro"},{"name":"Maeda Hiroshi"},{"name":"Otsuka Fumio"},{"name":"Takashiba Shogo"}],"ja":[{"name":"Omori Kazuhiro"},{"name":"Hanayama Yoshihisa"},{"name":"成石 浩司"},{"name":"Akiyama Kentaro"},{"name":"Maeda Hiroshi"},{"name":"Otsuka Fumio"},{"name":"Takashiba Shogo"}]},"description":{"en":"It has been suggested that vitamin C deficiency/scurvy is associated with gingival inflammatory changes; however, the disorder is very infrequently encountered in the modern era. Here, we report a case of extensive gingival overgrowth caused by vitamin C deficiency associated with metabolic syndrome and severe periodontal infection.","ja":"It has been suggested that vitamin C deficiency/scurvy is associated with gingival inflammatory changes; however, the disorder is very infrequently encountered in the modern era. Here, we report a case of extensive gingival overgrowth caused by vitamin C deficiency associated with metabolic syndrome and severe periodontal infection."},"publication_date":"2014-09-04","publication_name":{"en":"Clinical Case Reports","ja":"Clinical Case Reports"},"volume":"Vol.2","number":"No.6","starting_page":"286","ending_page":"295","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1002/ccr3.114"],"issn":["2050-0904"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=298084","label":"url"}],"paper_title":{"en":"ADL,認知機能の低下及び低栄養を併発した老年症候群患者は摂食嚥下機能が低下する","ja":"ADL,認知機能の低下及び低栄養を併発した老年症候群患者は摂食嚥下機能が低下する"},"authors":{"en":[{"name":"久保 克行"},{"name":"目黒 道夫"},{"name":"竹内 いずみ"},{"name":"中山 良子"},{"name":"加藤 真由美"},{"name":"Naruishi Koji"},{"name":"澤田 弘一"},{"name":"足立 誠司"},{"name":"山下 裕"}],"ja":[{"name":"久保 克行"},{"name":"目黒 道夫"},{"name":"竹内 いずみ"},{"name":"中山 良子"},{"name":"加藤 真由美"},{"name":"成石 浩司"},{"name":"澤田 弘一"},{"name":"足立 誠司"},{"name":"山下 裕"}]},"publication_date":"2014-06-01","publication_name":{"en":"全国自治体病院協議会雑誌","ja":"全国自治体病院協議会雑誌"},"volume":"Vol.53","number":"No.6","starting_page":"943","ending_page":"948","languages":["jpn"],"referee":true,"identifiers":{"issn":["0389-1070"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/23433894","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=280547","label":"url"}],"paper_title":{"en":"Combined therapeutic effects of adenoviral vector-mediated GLIPR1 gene therapy and radiotherapy in prostate and bladder cancer models.","ja":"Combined therapeutic effects of adenoviral vector-mediated GLIPR1 gene therapy and radiotherapy in prostate and bladder cancer models."},"authors":{"en":[{"name":"Fujita Tetsuo"},{"name":"Satoh Takefumi"},{"name":"Timme Terry L"},{"name":"Hirayama Takahiro"},{"name":"Zhu Julie X"},{"name":"Kusaka Nobuyuki"},{"name":"Naruishi Koji"},{"name":"Yang Guang"},{"name":"Goltsov Alexei"},{"name":"Wang Jianxiang"},{"name":"Vlachaki Maria T"},{"name":"Teh Bin S"},{"name":"Brian Butler E"},{"name":"Thompson Timothy C"}],"ja":[{"name":"Fujita Tetsuo"},{"name":"Satoh Takefumi"},{"name":"Timme Terry L"},{"name":"Hirayama Takahiro"},{"name":"Zhu Julie X"},{"name":"Kusaka Nobuyuki"},{"name":"成石 浩司"},{"name":"Yang Guang"},{"name":"Goltsov Alexei"},{"name":"Wang Jianxiang"},{"name":"Vlachaki Maria T"},{"name":"Teh Bin S"},{"name":"Brian Butler E"},{"name":"Thompson Timothy C"}]},"description":{"en":"Combining AdGlipr1 (AdGLIPR1) with radiotherapy may achieve additive or synergistic tumor control in selected prostate and bladder tumors, and additional therapeutic effects may result with repeated treatment cycles.","ja":"Combining AdGlipr1 (AdGLIPR1) with radiotherapy may achieve additive or synergistic tumor control in selected prostate and bladder tumors, and additional therapeutic effects may result with repeated treatment cycles."},"publication_date":"2014-02-20","publication_name":{"en":"Urologic Oncology: Seminars and Original Investigations","ja":"Urologic Oncology: Seminars and Original Investigations"},"volume":"Vol.32","number":"No.2","starting_page":"92","ending_page":"100","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1016/j.urolonc.2012.10.007"],"issn":["1873-2496"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/23428975","label":"url"},{"@id":"https://www.scopus.com/record/display.url?eid=2-s2.0-84874304488&origin=inward","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=280554","label":"url"}],"paper_title":{"en":"Secreted caveolin-1 enhances periodontal inflammation by targeting gingival fibroblasts.","ja":"Secreted caveolin-1 enhances periodontal inflammation by targeting gingival fibroblasts."},"authors":{"en":[{"name":"Takizawa Naoki"},{"name":"Sawada Shunsuke"},{"name":"Chosa Naoyuki"},{"name":"Ishisaki Akira"},{"name":"Naruishi Koji"}],"ja":[{"name":"Takizawa Naoki"},{"name":"Sawada Shunsuke"},{"name":"Chosa Naoyuki"},{"name":"Ishisaki Akira"},{"name":"成石 浩司"}]},"description":{"en":"Caveolin-1 (Cav-1) is a membrane protein. Recently, it has been reported that secreted Cav-1 induces angiogenesis in inflammatory microenvironment. However, it is unclear that Cav-1 regulates gingival inflammation. Therefore, we investigated the Cav-1 function to periodontal cells. Expression of Cav-1 in human periodontitis tissues was examined pathologically. Secretion of Cav-1 from human gingival fibroblasts (HGFs) or human periodontal ligament cells (HPLFs) treated with IL-1 and TNF- was examined using Western blotting. Likewise, intracellular signals induced by Cav-1 were examined. Finally, we examined whether the secreted Cav-1 induces production of inflammatory mediators in HGFs using ELISA or qRT-PCR. Pathologically, high expression of Cav-1 was observed in human periodontitis tissues. Cav-1 secretion increased in both cultured HGFs and HPLFs treated with IL-1 and TNF-. Cav-1 induced phosphorylation of JNK and ERK, but not Stat3 in HGFs. Furthermore, Cav-1 increased proMMP-1 and VEGF secretion in HGFs, and the VEGF secretion was statistically suppressed by JNK inhibitor SP600125, but not ERK inhibitor PD98059. ProMMP-1 secretion was suppressed statistically by both SP600125 and PD98059. In addition, Cav-1 increased significantly MMP-1, -10 and -14 mRNA expressions, whereas no increase of TIMPs mRNA was observed in HGFs treated with Cav-1. These data suggest that secreted Cav-1 derived from periodontal fibroblastic cells enhances inflammation-related several proteases and VEGF secretion in HGFs via MAPKs pathway, resulting in progression of periodontitis through induction of tissue degradation or angiogenesis.","ja":"Caveolin-1 (Cav-1) is a membrane protein. Recently, it has been reported that secreted Cav-1 induces angiogenesis in inflammatory microenvironment. However, it is unclear that Cav-1 regulates gingival inflammation. Therefore, we investigated the Cav-1 function to periodontal cells. Expression of Cav-1 in human periodontitis tissues was examined pathologically. Secretion of Cav-1 from human gingival fibroblasts (HGFs) or human periodontal ligament cells (HPLFs) treated with IL-1 and TNF- was examined using Western blotting. Likewise, intracellular signals induced by Cav-1 were examined. Finally, we examined whether the secreted Cav-1 induces production of inflammatory mediators in HGFs using ELISA or qRT-PCR. Pathologically, high expression of Cav-1 was observed in human periodontitis tissues. Cav-1 secretion increased in both cultured HGFs and HPLFs treated with IL-1 and TNF-. Cav-1 induced phosphorylation of JNK and ERK, but not Stat3 in HGFs. Furthermore, Cav-1 increased proMMP-1 and VEGF secretion in HGFs, and the VEGF secretion was statistically suppressed by JNK inhibitor SP600125, but not ERK inhibitor PD98059. ProMMP-1 secretion was suppressed statistically by both SP600125 and PD98059. In addition, Cav-1 increased significantly MMP-1, -10 and -14 mRNA expressions, whereas no increase of TIMPs mRNA was observed in HGFs treated with Cav-1. These data suggest that secreted Cav-1 derived from periodontal fibroblastic cells enhances inflammation-related several proteases and VEGF secretion in HGFs via MAPKs pathway, resulting in progression of periodontitis through induction of tissue degradation or angiogenesis."},"publication_date":"2013-02","publication_name":{"en":"Biomedical Research","ja":"Biomedical Research"},"volume":"Vol.34","number":"No.1","starting_page":"1","ending_page":"11","languages":["eng"],"referee":true,"identifiers":{"doi":["10.2220/biomedres.34.1"],"issn":["1880-313X"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/23428978","label":"url"},{"@id":"https://www.scopus.com/record/display.url?eid=2-s2.0-84874312974&origin=inward","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=280552","label":"url"}],"paper_title":{"en":"Enhancement of gingival inflammation induced by synergism of IL-1 and IL-6.","ja":"Enhancement of gingival inflammation induced by synergism of IL-1 and IL-6."},"authors":{"en":[{"name":"Sawada Shunsuke"},{"name":"Chosa Naoyuki"},{"name":"Ishisaki Akira"},{"name":"Naruishi Koji"}],"ja":[{"name":"Sawada Shunsuke"},{"name":"Chosa Naoyuki"},{"name":"Ishisaki Akira"},{"name":"成石 浩司"}]},"description":{"en":"Internleukin-1 (IL-1) and IL-6 are the most potent proinflammatory cytokines being involved in inflammatory diseases such as periodontitis. The objective of this study was to examine the synergistic effects of IL-1 and IL-6 on gingival inflammation by targeting cultured human gingival fibroblasts (HGFs). HGFs were treated with IL-1 or IL-6/soluble IL-6R (sIL-6R), and total RNA and total cell lysate were collected to examine expression of gp130 known as a signal transducer of IL-6 using qRT-PCR and Western blotting. IL-1-mediated IL-6 productivity in HGFs was examined using ELISA method. Likewise, after HGFs and THP-1 macrophages were treated with IL-1, TNF- and IL-6, sIL-6R productivity was examined. Next, HGFs were treated with IL-6/ sIL-6R after pretreatment of IL-1, and the intracellular signals were examined using Western blotting. Finally, various mRNA/protein expressions in HGFs treated with IL-6/sIL-6R after pretreatment of IL-1 were examined using qRT-PCR and ELISA method. IL-1 increased significantly both gp130 and IL-6 expression in HGFs. IL-6 increased significantly sIL-6R production in THP-1 macrophages but not HGFs. Co-stimulation with IL-1 and IL-6/sIL-6R induced dramatically the phosphorylation of Stat3, ERK and JNK in HGFs. Interestingly, expression of various inflammation- related molecules such as MMP-1, MCP-1, IL-1ra, bFGF and VEGF were enhanced by co-stimulation with IL-1 and IL-6/sIL-6R in HGFs. Gingival inflammation is regulated by HGFs affected by both IL-1 and IL-6/sIL-6R synergistically through induction of gp130 expression, resulting in progression of periodontitis.","ja":"Internleukin-1 (IL-1) and IL-6 are the most potent proinflammatory cytokines being involved in inflammatory diseases such as periodontitis. The objective of this study was to examine the synergistic effects of IL-1 and IL-6 on gingival inflammation by targeting cultured human gingival fibroblasts (HGFs). HGFs were treated with IL-1 or IL-6/soluble IL-6R (sIL-6R), and total RNA and total cell lysate were collected to examine expression of gp130 known as a signal transducer of IL-6 using qRT-PCR and Western blotting. IL-1-mediated IL-6 productivity in HGFs was examined using ELISA method. Likewise, after HGFs and THP-1 macrophages were treated with IL-1, TNF- and IL-6, sIL-6R productivity was examined. Next, HGFs were treated with IL-6/ sIL-6R after pretreatment of IL-1, and the intracellular signals were examined using Western blotting. Finally, various mRNA/protein expressions in HGFs treated with IL-6/sIL-6R after pretreatment of IL-1 were examined using qRT-PCR and ELISA method. IL-1 increased significantly both gp130 and IL-6 expression in HGFs. IL-6 increased significantly sIL-6R production in THP-1 macrophages but not HGFs. Co-stimulation with IL-1 and IL-6/sIL-6R induced dramatically the phosphorylation of Stat3, ERK and JNK in HGFs. Interestingly, expression of various inflammation- related molecules such as MMP-1, MCP-1, IL-1ra, bFGF and VEGF were enhanced by co-stimulation with IL-1 and IL-6/sIL-6R in HGFs. Gingival inflammation is regulated by HGFs affected by both IL-1 and IL-6/sIL-6R synergistically through induction of gp130 expression, resulting in progression of periodontitis."},"publication_date":"2013-02","publication_name":{"en":"Biomedical Research","ja":"Biomedical Research"},"volume":"Vol.34","number":"No.1","starting_page":"31","ending_page":"40","languages":["eng"],"referee":true,"identifiers":{"doi":["10.2220/biomedres.34.31"],"issn":["1880-313X"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/23018816","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=281243","label":"url"}],"paper_title":{"en":"Assessment of the plasma/serum IgG test to screen for periodontitis.","ja":"Assessment of the plasma/serum IgG test to screen for periodontitis."},"authors":{"en":[{"name":"Kudo C"},{"name":"Naruishi Koji"},{"name":"Maeda H"},{"name":"Abiko Y"},{"name":"Hino T"},{"name":"Iwata M"},{"name":"Mitsuhashi C"},{"name":"Murakami S"},{"name":"Nagasawa T"},{"name":"Nagata Toshihiko"},{"name":"Yoneda Satoshi"},{"name":"Nomura Y"},{"name":"Noguchi T"},{"name":"Numabe Y"},{"name":"Ogata Y"},{"name":"Sato T"},{"name":"Shimauchi H"},{"name":"Yamazaki K"},{"name":"Yoshimura A"},{"name":"Takashiba S"}],"ja":[{"name":"Kudo C"},{"name":"成石 浩司"},{"name":"Maeda H"},{"name":"Abiko Y"},{"name":"Hino T"},{"name":"Iwata M"},{"name":"Mitsuhashi C"},{"name":"Murakami S"},{"name":"Nagasawa T"},{"name":"永田 俊彦"},{"name":"米田 哲"},{"name":"Nomura Y"},{"name":"Noguchi T"},{"name":"Numabe Y"},{"name":"Ogata Y"},{"name":"Sato T"},{"name":"Shimauchi H"},{"name":"Yamazaki K"},{"name":"Yoshimura A"},{"name":"Takashiba S"}]},"description":{"en":"Chronic periodontitis is a silent infectious disease prevalent worldwide and affects lifestyle-related diseases. Therefore, efficient screening of patients is essential for general health. This study was performed to evaluate prospectively the diagnostic utility of a blood IgG antibody titer test against periodontal pathogens. Oral examination was performed, and IgG titers against periodontal pathogens were measured by ELISA in 1,387 individuals. The cut-off value of the IgG titer was determined in receiver operating characteristic curve analysis, and changes in periodontal clinical parameters and IgG titers by periodontal treatment were evaluated. The relationships between IgG titers and severity of periodontitis were analyzed. The best cut-off value of IgG titer against Porphyromonas gingivalis for screening periodontitis was 1.682. Both clinical parameters and IgG titers decreased significantly under periodontal treatment. IgG titers of periodontitis patients were significantly higher than those of healthy controls, especially in those with sites of probing pocket depth over 4 mm. Multiplied cut-off values were useful to select patients with severe periodontitis. A blood IgG antibody titer test for Porphyromonas gingivalis is useful to screen hitherto chronic periodontitis patients.","ja":"Chronic periodontitis is a silent infectious disease prevalent worldwide and affects lifestyle-related diseases. Therefore, efficient screening of patients is essential for general health. This study was performed to evaluate prospectively the diagnostic utility of a blood IgG antibody titer test against periodontal pathogens. Oral examination was performed, and IgG titers against periodontal pathogens were measured by ELISA in 1,387 individuals. The cut-off value of the IgG titer was determined in receiver operating characteristic curve analysis, and changes in periodontal clinical parameters and IgG titers by periodontal treatment were evaluated. The relationships between IgG titers and severity of periodontitis were analyzed. The best cut-off value of IgG titer against Porphyromonas gingivalis for screening periodontitis was 1.682. Both clinical parameters and IgG titers decreased significantly under periodontal treatment. IgG titers of periodontitis patients were significantly higher than those of healthy controls, especially in those with sites of probing pocket depth over 4 mm. Multiplied cut-off values were useful to select patients with severe periodontitis. A blood IgG antibody titer test for Porphyromonas gingivalis is useful to screen hitherto chronic periodontitis patients."},"publication_date":"2012-09-26","publication_name":{"en":"Journal of Dental Research","ja":"Journal of Dental Research"},"volume":"Vol.91","number":"No.12","starting_page":"1190","ending_page":"1195","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1177/0022034512461796"],"issn":["1544-0591"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/22792372","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=280555","label":"url"}],"paper_title":{"en":"Relationship between periodontitis-related antibody and frequent exacerbations in chronic obstructive pulmonary disease.","ja":"Relationship between periodontitis-related antibody and frequent exacerbations in chronic obstructive pulmonary disease."},"authors":{"en":[{"name":"Takahashi Tamaki"},{"name":"Muro Shigeo"},{"name":"Tanabe Naoya"},{"name":"Terada Kunihiko"},{"name":"Kiyokawa Hirofumi"},{"name":"Sato Susumu"},{"name":"Hoshino Yuma"},{"name":"Ogawa Emiko"},{"name":"Uno Kazuko"},{"name":"Naruishi Koji"},{"name":"Takashiba Shogo"},{"name":"Mishima Michiaki"}],"ja":[{"name":"Takahashi Tamaki"},{"name":"Muro Shigeo"},{"name":"Tanabe Naoya"},{"name":"Terada Kunihiko"},{"name":"Kiyokawa Hirofumi"},{"name":"Sato Susumu"},{"name":"Hoshino Yuma"},{"name":"Ogawa Emiko"},{"name":"Uno Kazuko"},{"name":"成石 浩司"},{"name":"Takashiba Shogo"},{"name":"Mishima Michiaki"}]},"description":{"en":"Normal-IgG titer for periodontitis-related antibody can be an independent predictor of frequent exacerbations. Measuring periodontitis-related antibody titers might be useful to identify patients with susceptibility to frequent exacerbations so that an aggressive prevention strategy can be designed.","ja":"The numbers of exacerbations and the rate of individuals with frequent exacerbations (at least two per year) were significantly lower in patients with higher IgG titer than those with normal IgG titer (0.8 vs. 1.2 per year, p= 0.045 and 14.3 vs. 38.6%, p= 0.009, respectively). Multivariate logistic regression analysis showed that being normal-IgG titer for periodontitis-related antibody significantly increased the risk of frequent exacerbations (relative risk, 5.27, 95% confidence interval, 1.30-25.7; p = 0.019) after adjusting for other possible confounders, such as a history of exacerbations in the past year, disease severity, COPD medication and smoking status."},"publication_date":"2012-07-11","publication_name":{"en":"PLoS ONE","ja":"PLoS ONE"},"volume":"Vol.7","number":"No.7","starting_page":"e40570","ending_page":"e40570","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1371/journal.pone.0040570"],"issn":["1932-6203"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/21525188","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=280976","label":"url"}],"paper_title":{"en":"Specific in situ visualization of plasma cells producing antibodies against Porphyromonas gingivalis in gingival radicular cyst: application of the enzyme-labeled antigen method.","ja":"Specific in situ visualization of plasma cells producing antibodies against Porphyromonas gingivalis in gingival radicular cyst: application of the enzyme-labeled antigen method."},"authors":{"en":[{"name":"Tsuge Shinya"},{"name":"Mizutani Yasuyoshi"},{"name":"Matsuoka Kazuhiro"},{"name":"Sawasaki Tatsuya"},{"name":"Endo Yaeta"},{"name":"Naruishi Koji"},{"name":"Maeda Hiroshi"},{"name":"Takashiba Shogo"},{"name":"Shiogama Kazuya"},{"name":"Inada Ken-Ichi"},{"name":"Tsutsumi Yutaka"}],"ja":[{"name":"Tsuge Shinya"},{"name":"Mizutani Yasuyoshi"},{"name":"Matsuoka Kazuhiro"},{"name":"Sawasaki Tatsuya"},{"name":"Endo Yaeta"},{"name":"成石 浩司"},{"name":"Maeda Hiroshi"},{"name":"Takashiba Shogo"},{"name":"Shiogama Kazuya"},{"name":"Inada Ken-Ichi"},{"name":"Tsutsumi Yutaka"}]},"description":{"en":"The enzyme-labeled antigen method was applied to visualize plasma cells producing antibodies to Porphyromonas gingivalis, flora of the human oral cavity. Antibodies to P. gingivalis have reportedly been detected in sera of patients with periodontitis. Biotinylated bacterial antigens, Ag53, and four gingipain domains (Arg-pro, Arg-hgp, Lys-pro, and Lys-hgp) were prepared by the cell-free protein synthesis system using the wheat germ extract. In paraformaldehyde-fixed frozen sections of rat lymph nodes experimentally immunized with Ag53-positive and Ag53-negative P. gingivalis, plasma cells were labeled with biotinylated Arg-hgp and Lys-hgp. Antibodies to Ag53 were detected only in the nodes immunized with Ag53-positive bacteria. In two of eight lesions of gingival radicular cyst with inflammatory infiltration, CD138-positive plasma cells in frozen sections were signalized for Arg-hgp and Lys-hgp. An absorption study using unlabeled antigens confirmed the specificity of staining. The AlphaScreen method identified the same-type antibodies in tissue extracts but not in sera. Antibodies to Ag53, Arg-pro, and Lys-pro were undetectable. In two cases, serum antibodies to Arg-hgp and Lys-hgp were AlphaScreen positive, whereas plasma cells were scarcely observed within the lesions. These findings indicate the validity of the enzyme-labeled antigen method. This is the very first application of this novel histochemical technique to human clinical samples.","ja":"The enzyme-labeled antigen method was applied to visualize plasma cells producing antibodies to Porphyromonas gingivalis, flora of the human oral cavity. Antibodies to P. gingivalis have reportedly been detected in sera of patients with periodontitis. Biotinylated bacterial antigens, Ag53, and four gingipain domains (Arg-pro, Arg-hgp, Lys-pro, and Lys-hgp) were prepared by the cell-free protein synthesis system using the wheat germ extract. In paraformaldehyde-fixed frozen sections of rat lymph nodes experimentally immunized with Ag53-positive and Ag53-negative P. gingivalis, plasma cells were labeled with biotinylated Arg-hgp and Lys-hgp. Antibodies to Ag53 were detected only in the nodes immunized with Ag53-positive bacteria. In two of eight lesions of gingival radicular cyst with inflammatory infiltration, CD138-positive plasma cells in frozen sections were signalized for Arg-hgp and Lys-hgp. An absorption study using unlabeled antigens confirmed the specificity of staining. The AlphaScreen method identified the same-type antibodies in tissue extracts but not in sera. Antibodies to Ag53, Arg-pro, and Lys-pro were undetectable. In two cases, serum antibodies to Arg-hgp and Lys-hgp were AlphaScreen positive, whereas plasma cells were scarcely observed within the lesions. These findings indicate the validity of the enzyme-labeled antigen method. This is the very first application of this novel histochemical technique to human clinical samples."},"publication_date":"2011-04-27","publication_name":{"en":"The Journal of Histochemistry and Cytochemistry","ja":"The Journal of Histochemistry and Cytochemistry"},"volume":"Vol.59","number":"No.7","starting_page":"673","ending_page":"689","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1369/0022155411408906"],"issn":["1551-5044"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/21880208","label":"url"},{"@id":"https://www.scopus.com/record/display.url?eid=2-s2.0-80052897323&origin=inward","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=280583","label":"url"}],"paper_title":{"en":"Immune responses to porphyromonas gingivalis infection suppress systemic inflammatory response in experimental murine model.","ja":"Immune responses to porphyromonas gingivalis infection suppress systemic inflammatory response in experimental murine model."},"authors":{"en":[{"name":"Naruishi Koji"},{"name":"Omori K"},{"name":"Maeda H"},{"name":"Sonoi N"},{"name":"Funakoshi K"},{"name":"Hirai K"},{"name":"Ishii M"},{"name":"Kubo K"},{"name":"Kobayashi H"},{"name":"Tomiyama T"},{"name":"Yamamoto D"},{"name":"Tanimoto I"},{"name":"Kunimatsu K"},{"name":"Takashiba S"}],"ja":[{"name":"成石 浩司"},{"name":"Omori K"},{"name":"Maeda H"},{"name":"Sonoi N"},{"name":"Funakoshi K"},{"name":"Hirai K"},{"name":"Ishii M"},{"name":"Kubo K"},{"name":"Kobayashi H"},{"name":"Tomiyama T"},{"name":"Yamamoto D"},{"name":"Tanimoto I"},{"name":"Kunimatsu K"},{"name":"Takashiba S"}]},"description":{"en":"Periodontitis is a localized infectious disease caused by periodontopathic bacteria such as Porphyromonas gingivalis (P. gingivalis), and the severity correlates to significance of immune responses. Recently, it has been reported that periodontitis is associated with the development of systemic disease such as diabetes and atherosclerosis because of increasing invasion of oral pathogens to the circulation. However, the association between local and systemic infectious responses is still unclear. In the present study, we examined the differences of biological responses in animals with or without bacterial infection. After Balb/c mice were infected subcutaneously with live P. gingivalis W83, serum, skin and liver were collected according to experimental protocol. The skin and liver tissues were observed pathologically by haematoxylin-eosin staining, and serum IL-6 levels were measured using ELISA method. Throughout the experimental period, conditions of the mice were observed continuously. As expected, severe infiltration of leukocytes were observed at inflamed skin corresponding to the number of bacterial challenges. Although no inflammatory appearance of skin was observed, serum IL-6 levels were increased dramatically (P <0.01, Student's t-test) and liver tissues were injured in the mice without bacterial challenge. Interestingly, although severe inflammatory appearance of the skin was observed, serum IL-6 levels were not increased and no inflammatory responses were observed in the liver of the 3-times bacterially challenged group. Importantly, immunoglobulin G against P. gingivalis W83 was detected in the blood of mice with 3-times bacterial challenge corresponding to improvement of weight loss and survival. In conclusion, although multiple infections develop severe localized inflammation, the immune system should be sufficient to protect the systemic inflammatory responses.","ja":"Periodontitis is a localized infectious disease caused by periodontopathic bacteria such as Porphyromonas gingivalis (P. gingivalis), and the severity correlates to significance of immune responses. Recently, it has been reported that periodontitis is associated with the development of systemic disease such as diabetes and atherosclerosis because of increasing invasion of oral pathogens to the circulation. However, the association between local and systemic infectious responses is still unclear. In the present study, we examined the differences of biological responses in animals with or without bacterial infection. After Balb/c mice were infected subcutaneously with live P. gingivalis W83, serum, skin and liver were collected according to experimental protocol. The skin and liver tissues were observed pathologically by haematoxylin-eosin staining, and serum IL-6 levels were measured using ELISA method. Throughout the experimental period, conditions of the mice were observed continuously. As expected, severe infiltration of leukocytes were observed at inflamed skin corresponding to the number of bacterial challenges. Although no inflammatory appearance of skin was observed, serum IL-6 levels were increased dramatically (P <0.01, Student's t-test) and liver tissues were injured in the mice without bacterial challenge. Interestingly, although severe inflammatory appearance of the skin was observed, serum IL-6 levels were not increased and no inflammatory responses were observed in the liver of the 3-times bacterially challenged group. Importantly, immunoglobulin G against P. gingivalis W83 was detected in the blood of mice with 3-times bacterial challenge corresponding to improvement of weight loss and survival. In conclusion, although multiple infections develop severe localized inflammation, the immune system should be sufficient to protect the systemic inflammatory responses."},"publication_date":"2011-04","publication_name":{"en":"Journal of Biological Regulators and Homeostatic Agents","ja":"Journal of Biological Regulators and Homeostatic Agents"},"volume":"Vol.25","number":"No.2","starting_page":"195","ending_page":"202","languages":["eng"],"referee":true,"identifiers":{"issn":["0393-974X"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://cir.nii.ac.jp/crid/1050845762641886336/","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=281505","label":"url"}],"paper_title":{"en":"臨床研究 医科歯科連携医療における歯周病原細菌に対する血清IgG抗体価検査の導入","ja":"臨床研究 医科歯科連携医療における歯周病原細菌に対する血清IgG抗体価検査の導入"},"authors":{"en":[{"name":"村井 治"},{"name":"Naruishi Koji"},{"name":"佐々木 大輔"},{"name":"大川 義人"},{"name":"八重柏 隆"},{"name":"國松 和司"}],"ja":[{"name":"村井 治"},{"name":"成石 浩司"},{"name":"佐々木 大輔"},{"name":"大川 義人"},{"name":"八重柏 隆"},{"name":"國松 和司"}]},"publication_date":"2011","publication_name":{"en":"Journal of Japanese Society for Evidence and the Dental Professional","ja":"日本口腔検査学会雑誌"},"volume":"Vol.3","number":"No.1","starting_page":"36","ending_page":"41","languages":["jpn"],"referee":true,"identifiers":{"issn":["1883-3888"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/21254239","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=280977","label":"url"}],"paper_title":{"en":"Prognosis of periodontitis recurrence after intensive periodontal treatment using examination of serum IgG antibody titer against periodontal bacteria.","ja":"Prognosis of periodontitis recurrence after intensive periodontal treatment using examination of serum IgG antibody titer against periodontal bacteria."},"authors":{"en":[{"name":"Sugi Noriko"},{"name":"Naruishi Koji"},{"name":"Kudo Chieko"},{"name":"Hisaeda-Kako Aya"},{"name":"Kono Takayuki"},{"name":"Maeda Hiroshi"},{"name":"Takashiba Shogo"}],"ja":[{"name":"Sugi Noriko"},{"name":"成石 浩司"},{"name":"Kudo Chieko"},{"name":"Hisaeda-Kako Aya"},{"name":"Kono Takayuki"},{"name":"Maeda Hiroshi"},{"name":"Takashiba Shogo"}]},"description":{"en":"Chronic periodontitis is associated with systemic diseases such as atherosclerosis. In this study, we evaluated the efficacy of serum IgG antibody titer to periodontal bacteria for prognosis of periodontitis recurrence during supportive periodontal therapy (SPT) phase. The 139 patients during SPT phase were selected and divided to two groups as follows: \"Stable\" and \"Recurrence\" group at SPT phase for case-control study: \"High IgG titer\" and \"Normal IgG titer\" group before transition to SPT phase for cohort study. We examined whether clinical findings or serum IgG antibody titers to periodontal bacteria are risk factors for the development of periodontitis recurrence. Case-control study showed that there were significant differences between the stable and recurrence groups in age and number of teeth. The serum IgG antibody titer to Eikenella corrodens FDC1073, Porphyromonas gingivalis SU63, and Campylobacter rectus ATCC33238 was significantly higher in the recurrence group. Next, we found, that the recurrence ratio in the high IgG titer group to Gram-negative obligate anaerobe, Prevotella intermedia, Treponema denticola, and C. rectus was significantly higher than that of the normal IgG titer group. Taken together, serum IgG antibody titer test is useful in the prognosis of periodontitis recurrence during the SPT phase.","ja":"Chronic periodontitis is associated with systemic diseases such as atherosclerosis. In this study, we evaluated the efficacy of serum IgG antibody titer to periodontal bacteria for prognosis of periodontitis recurrence during supportive periodontal therapy (SPT) phase. The 139 patients during SPT phase were selected and divided to two groups as follows: \"Stable\" and \"Recurrence\" group at SPT phase for case-control study: \"High IgG titer\" and \"Normal IgG titer\" group before transition to SPT phase for cohort study. We examined whether clinical findings or serum IgG antibody titers to periodontal bacteria are risk factors for the development of periodontitis recurrence. Case-control study showed that there were significant differences between the stable and recurrence groups in age and number of teeth. The serum IgG antibody titer to Eikenella corrodens FDC1073, Porphyromonas gingivalis SU63, and Campylobacter rectus ATCC33238 was significantly higher in the recurrence group. Next, we found, that the recurrence ratio in the high IgG titer group to Gram-negative obligate anaerobe, Prevotella intermedia, Treponema denticola, and C. rectus was significantly higher than that of the normal IgG titer group. Taken together, serum IgG antibody titer test is useful in the prognosis of periodontitis recurrence during the SPT phase."},"publication_date":"2011","publication_name":{"en":"Journal of Clinical Laboratory Analysis","ja":"Journal of Clinical Laboratory Analysis"},"volume":"Vol.25","number":"No.1","starting_page":"25","ending_page":"32","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1002/jcla.20381"],"issn":["1098-2825"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/21888018","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=280558","label":"url"}],"paper_title":{"en":"Cathepsin B, D, and L regulation in cyclosporin A-mediated gingival hyperplasia of a patient with sarcoidosis.","ja":"Cathepsin B, D, and L regulation in cyclosporin A-mediated gingival hyperplasia of a patient with sarcoidosis."},"authors":{"en":[{"name":"Murai Osamu"},{"name":"Naruishi Koji"},{"name":"Ogihara Satoshi"},{"name":"Suwa Nagisa"},{"name":"Kanazawa Satomi"},{"name":"Yaegashi Takashi"},{"name":"Takeda Yasunori"},{"name":"Kunimatsu Kazushi"}],"ja":[{"name":"Murai Osamu"},{"name":"成石 浩司"},{"name":"Ogihara Satoshi"},{"name":"Suwa Nagisa"},{"name":"Kanazawa Satomi"},{"name":"Yaegashi Takashi"},{"name":"Takeda Yasunori"},{"name":"Kunimatsu Kazushi"}]},"description":{"en":"Cyclosporin A (CsA) is an immunosuppressant with side effects including gingival hyperplasia. Sarcoidosis is a systemic disease characterized by granulomas. Here, we report on a rare case of sarcoidosis with gingival hyperplasia to clarify whether clinical observation corresponds to in vitro results. Gingival fibroblasts (HGFs) were isolated from healthy gingiva and cultured with CsA. Total RNA was collected and expression of mRNAs examined using semi-quantitative RT-PCR analysis. Cathepsin B, D, and L expression in overgrown gingiva of the patient was examined by immunohistochemistry. Cathepsin D, L, and vascular endothelial growth factor (VEGF)165 mRNA were markedly suppressed in CsA-treated HGFs, whereas cathepsin B, matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA were not reduced. Next, the decrease of cathepsin B and L expression in enlarged gingiva was observed, whereas an increase of cathepsin D expression was observed. Clinically, the enlarged gingival lesions were fully resolved by performing oral infection control. Cathepsins regulation might be an important factor in the development of CsA-mediated gingival hyperplasia.","ja":"Cyclosporin A (CsA) is an immunosuppressant with side effects including gingival hyperplasia. Sarcoidosis is a systemic disease characterized by granulomas. Here, we report on a rare case of sarcoidosis with gingival hyperplasia to clarify whether clinical observation corresponds to in vitro results. Gingival fibroblasts (HGFs) were isolated from healthy gingiva and cultured with CsA. Total RNA was collected and expression of mRNAs examined using semi-quantitative RT-PCR analysis. Cathepsin B, D, and L expression in overgrown gingiva of the patient was examined by immunohistochemistry. Cathepsin D, L, and vascular endothelial growth factor (VEGF)165 mRNA were markedly suppressed in CsA-treated HGFs, whereas cathepsin B, matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA were not reduced. Next, the decrease of cathepsin B and L expression in enlarged gingiva was observed, whereas an increase of cathepsin D expression was observed. Clinically, the enlarged gingival lesions were fully resolved by performing oral infection control. Cathepsins regulation might be an important factor in the development of CsA-mediated gingival hyperplasia."},"publication_date":"2011","publication_name":{"en":"Clinical Laboratory","ja":"Clinical Laboratory"},"volume":"Vol.57","number":"No.7-8","starting_page":"535","ending_page":"541","languages":["eng"],"referee":true,"identifiers":{"issn":["1433-6510"]},"published_paper_type":"scientific_journal"}} {"insert":{"user_id":"B000342791","type":"published_papers"},"force":{"see_also":[{"@id":"https://cir.nii.ac.jp/crid/1390001204411451008/","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=281535","label":"url"}],"paper_title":{"en":"Clinical and microbiological effect of newly developed OTC periodontal ointment-containing applicator with brush on the SPT phase","ja":"サポーティブペリオドンタルセラピー期における塗布用ブラシ一体型一般用歯周病薬の臨床的および細菌学的な効果"},"authors":{"en":[{"name":"INUBUSHI Junya"},{"name":"HATANAKA Kazu"},{"name":"YASUDA Takako"},{"name":"Naruishi Koji"},{"name":"OOTSUKI Hidehiko"},{"name":"TAKASHIBA Shogo"}],"ja":[{"name":"犬伏 順也"},{"name":"畑中 加珠"},{"name":"安田 多賀子"},{"name":"成石 浩司"},{"name":"大槻 秀彦"},{"name":"高柴 正悟"}]},"description":{"en":"It is important for maintaining improved periodontal tissue health during supportive periodontal therapy (SPT) to restrain infection caused by periodontopathic bacteria by the patients' own self control in addition to periodic professional intervention. We developed an over-the-counter (OTC) medication, named MC1, consisting of a one-tuft brush containing the compound cetylpyridinium chloride (CPC) as a bactericidal agent, dipotassium glycyrrhizate (GK2) as an anti-inflammatory drug, and allantoin (ALT) as a promoter of cell proliferation and wound healing, for delivery to hardly-brushed regions. In this study, we evaluated the effectiveness of MC1 clinically and microbiologically in maintaining improved periodontal tissue status during SPT.