type: misc

label: url

en: Recent trends in the field of lipid engineering

en: Lipid engineering related to biological functions has made remarkable progress in the fields of microbial production of functional lipids, metabolic engineering of microorganisms, elucidation of physiological functions of rare lipids, lipid-related enzyme engineering, and lipid analysis techniques. Various rare lipids are produced by utilizing microorganisms and their enzymes. It is also becoming clear that the rare lipids produced by intestinal bacteria contribute significantly to human health. Technological advances related to identification of lipid structures and quantification of lipids have led to such discoveries in the field of lipid engineering. This article reviews the latest findings that are attracting attention in the field of lipid engineering related to biological functions.

en: Journal of Bioscience and Bioengineering


en: Production of Various ω3-polyunsaturated Fatty Acid-containing Lipids by Oleaginous Microorganisms

ja: 油糧微生物による種々のω3脂肪酸含有油脂の発酵生産

en: BIO INDUSTRY

ja: 月刊バイオインダストリー


en: 高度不飽和脂肪酸・共役脂肪酸含有油脂の微生物生産

ja: 高度不飽和脂肪酸・共役脂肪酸含有油脂の微生物生産

en: 微生物を活用した新世代の有用物質生産技術《普及版》

ja: 微生物を活用した新世代の有用物質生産技術《普及版》


en: Arachidonic acid production by the oleaginous fungus Mortierella alpina 1S-4: A review

en: The filamentous fungus Mortierella alpina 1S-4 is capable of accumulating a large amount of triacylglycerol containing C20 polyunsaturated fatty acids (PUFAs). Indeed, triacylglycerol production by M. alpina 1S-4 can reach 20 g/L of culture broth, and the critical cellular signaling and structural PUFA arachidonic acid (ARA) comprises 30% 70% of the total fatty acid. The demonstrated health benefits of functional PUFAs have in turn encouraged the search for rich sources of these compounds, including fungal strains showing enhanced production of specific PUFAs. Screening for mutants and targeted gene manipulation of M. alpina 1S-4 have elucidated the functions of various enzymes involved in PUFA biosynthesis and established lines with improved PUFA productivity. In some cases, these strains have been used for indistrial-scale production of PUFAs, including ARA. In this review, we described practical ARA production through mutant breeding, functional analyses of genes encoding enzymes involved in PUFA biosynthesis, and recent advances in the production of specific PUFAs through molecular breeding of M. alpina 1S-4.

en: Journal of Advanced Research

ja: Journal of Advanced Research


en: Metabolic engineering for the production of polyunsaturated fatty acids by oleaginous fungus Mortierella alpina 1S-4

en: Researches related with the application of functional lipids such as polyunsaturated fatty acids (PUFAs) have been conducted in various fields with a view to health and dietary requirements. Novel rich sources other than known natural sources such as plant seeds and fish oils are required for increasing demands of PUFAs. The filamentous fungus Mortierella alpina 1S-4 produces triacylglycerols rich in arachidonic acid, i.e., ones reaching 20 g/l in concentration and containing 30-70% arachidonic acid as total fatty adds. Various mutants derived from M. alpina 1S-4 have led to the production of oils containing various PUFAs. Molecular breeding of M. alpina strains by means of manipulation of the genes involved in PUFA biosynthesis facilitates improvement of PUFA productivity and elucidation of the functions of their enzymes. This review describes practical PUFA production through mutant breeding, functional analyses of the genes of the enzymes involved in PUFA biosynthesis, and recent advances in unique PUFA production through molecular breeding.

ja: Researches related with the application of functional lipids such as polyunsaturated fatty acids (PUFAs) have been conducted in various fields with a view to health and dietary requirements. Novel rich sources other than known natural sources such as plant seeds and fish oils are required for increasing demands of PUFAs. The filamentous fungus Mortierella alpina 1S-4 produces triacylglycerols rich in arachidonic acid, i.e., ones reaching 20 g/l in concentration and containing 30-70% arachidonic acid as total fatty acids. Various mutants derived from M. alpina 1S-4 have led to the production of oils containing various PUFAs. Molecular breeding of M. alpina strains by means of manipulation of the genes involved in PUFA biosynthesis facilitates improvement of PUFA productivity and elucidation of the functions of their enzymes. This review describes practical PUFA production through mutant breeding, functional analyses of the genes of the enzymes involved in PUFA biosynthesis, and recent advances in unique PUFA production through molecular breeding.

ja: Journal of Bioscience and Bioengineering


en: Production and Transformation of Omega-3 Fatty Acid-containing Lipids by Microorganisms

ja: オメガ3脂肪酸含有油脂の微生物による生産と変換

ja: 月刊バイオインダストリー


en: Polyunsaturated fatty acids production and transformation by Mortierella alpina and anaerobic bacteria

en: European Journal of Lipid Science and Technology : EJLST


en: Production of Microbial Lipids Containing Arachidonic Acid and Its Related Polyunsaturated Fatty Acids

ja: アラキドン酸および関連高度不飽和脂肪酸を含む微生物油脂生産

en: The researches of the application of functional lipids such as polyunsaturated fatty acids (PUFAs) have been conducted in various fields with a view to health and nutrition. The sources of remarkable PUFAs with more than carbon 20 were arachidonic acid from eggs and n-3 PUFAs from fish oils. The filamentous fungus Mortierella alpina 1S-4, which was found on screening of microorganisms accumulating PUFAs with more than carbon 20, produces triacylglycerols rich in arachidonic acid, i.e., ones reaching 20 g/l in concentration and containing 30-70% arachidonic acid as total fatty acids. Various mutants derived from M. alpina 1S-4 have led to the production of oils containing various PUFAs. Molecular breeding of M. alpina strains by means of manipulation of the genes involved in PUFA biosynthesis facilitates improvement of PUFA productivity and elucidation of the functions of their enzymes. This review describes practical production of arachidonic acid and its related PUFAs through mutant breeding, functional analyses of the genes of the enzymes involved in PUFA biosynthesis, and recent advances in unique PUFA production through molecular breeding.

ja: 高度不飽和脂肪酸(PUFA)のような機能性脂質の応用研究は，「健康」 や 「栄養」 に関わる様々な分野で行われてきた．特に注目されてきた炭素鎖長20以上のPUFAの供給源は，鶏卵に含まれるアラキドン酸や魚油に含まれるn-3 PUFAが主なものであった．炭素鎖長20以上のPUFAを生産する微生物スクリーニングにより，アラキドン酸を豊富に含むトリアシルグリセロールを生産する油糧微生物Mortierella alpina 1S-4が見いだされた．M. alpina 1S-4において，油脂の生産量は20 g/lに達し，総脂肪酸に占めるアラキドン酸の割合は30∼70%を占める．これまでに，M. alpina 1S-4から様々な変異株が誘導され，多様なPUFAが生産されるようになった．さらに，M. alpina 1S-4の形質転換系の開発により，PUFA生合成に関わる酵素遺伝子の解明やPUFA生産の向上が可能となった．本稿では，M. alpina 1S-4の育種(変異株育種と分子育種を含む)によるアラキドン酸とその関連PUFAの実用的な微生物生産について報告する．

en: Oleoscience

ja: オレオサイエンス


en: バイオプラ最前線-バイオマスからのモノマー生産を支援するバイオリピッドプラットフォームの構築-

en: バイオプラジャーナル

ja: バイオプラジャーナル


en: 代謝的視点からの機能探索に基づく産業プロセス用酵素開発

ja: 代謝的視点からの機能探索に基づく産業プロセス用酵素開発

ja: 月刊バイオインダストリー


en: 第6回 油脂生産性糸状菌Mortierella alpinaによる機能性脂質生産 連載「微生物の産業利用-はたらく有用微生物-」

en: Microbiol. Cult. Coll.

ja: Microbiol. Cult. Coll.


en: Advances in the production of various polyunsaturated fatty acids through oleaginous fungus Mortierella alpina breeding

en: Studies of the application of functional lipids such as polyunsaturated fatty acids (PUFAs) have been conducted in various fields with a view to health and dietary requirements in a search for novel, rich sources. The filamentous fungus Mortierella alpina 1S-4 produces triacylglycerols rich in arachidonic acid, i.e., ones reaching 20 g/l in concentration and containing 30-70% arachidonic acid as total fatty acids. Various mutants derived from M. alpina 1S-4 have led to the production of oils containing various PUFAs. Molecular breeding of M. alpina strains by means of manipulation of the genes involved in PUFA biosynthesis facilitates improvement of PUFA productivity and elucidation of the functions of their enzymes. This review describes practical PUFA production through mutant breeding, functional analyses of the genes of the enzymes involved in PUFA biosynthesis, and recent advances in unique PUFA production through molecular breeding.

en: Bioscience, Biotechnology, and Biochemistry


en: Single cell oil production by Mortierella alpina

en: A filamentous fungus, Mortierella alpina 1S-4, was obtained, through extensive screening, as an potential producer of triacylglycerol containing C20 polyunsaturated fatty acids (PUFAs) such as arachidonic acid. With this discovery as a starting point, we conducted employing methods from metabolic engineering and molecular biology for controlling cultures and breeding mutant strains. These parental and mutant strains are now used for large-scale production of a variety of PUFAs.

en: Journal of Biotechnology

ja: Journal of Biotechnology


en: Improved production of various polyunsaturated fatty acids through filamentous fungus Mortierella alpina breeding

en: Studies on the application of functional lipids such as polyunsaturated fatty acids (PUFAs) have proceeded in various fields regarding health and dietary requirements in a search for novel and rich sources. Filamentous fungus Mortierella alpina 1S-4 produces triacylglycerols rich in arachidonic acid, ones reaching 20 g/L and containing 30-70% arachidonic acid as to the total fatty acids. Mutants derived from M. alpina 1S-4, defective in Delta5 and Delta6 desaturases, accumulate triacylglycerols rich in unique PUFAs, i.e., dihomo-gamma-linolenic acid and Mead acid, respectively. Furthermore, various mutants derived from M. alpina 1S-4 have led to the production of oils containing n-1, n-3, n-4, n-6, n-7, and n-9 PUFAs. A variety of genes encoding fatty acid desaturases and elongases involved in PUFA biosynthesis in M. alpina 1S-4 has been isolated and characterized. Molecular breeding of M. alpina strains by means of manipulation of these genes facilitates improvement of PUFA productivity and elucidation of the functions of enzymes involved in PUFA biosynthesis.

en: Applied Microbiology and Biotechnology

ja: Applied Microbiology and Biotechnology


en: Production of functional lipids by microorganisms: arachidonic acid and related polyunsaturated fatty acids

ja: 機能性脂質の微生物による生産. アラキドン酸に関連する油脂の発酵生産を中心として

en: Tanpakushitsu Kakusan Koso

ja: 蛋白質 核酸 酵素


en: 機能性脂質の微生物生産

ja: 機能性脂質の微生物生産

en: Seibutsu-kogaku Kaishi

ja: 生物工学会誌


en: 機能性脂質の微生物生産-アラキドン酸関連高度不飽和脂肪酸含有油脂および共役脂肪酸の生産を中心として-

en: Oleoscience


en: 微生物による機能性脂質の生産

ja: 微生物による機能性脂質の生産

en: 科学と工業

ja: 科学と工業


en: 有用油脂生産微生物の代謝工学

ja: 有用油脂生産微生物の代謝工学

en: Bioscience & Industry

ja: バイオサイエンスとインダストリー


en: Desaturase defective fungal mutants: useful tools for the regulation and overproduction of polyunsaturated fatty acids

en: Trends in Biotechnol.

ja: Trends in Biotechnol.


en: 令和2年度 徳島大学高等教育研究センターアドミッション部門 報告書

ja: 令和2年度 徳島大学高等教育研究センターアドミッション部門 報告書

en: 令和2年度 徳島大学高等教育研究センターアドミッション部門 報告書

ja: 令和2年度 徳島大学高等教育研究センターアドミッション部門 報告書

misc_type: others