徳島大学 / 教育研究者総覧 --- 清水紀之

専門分野

○	環境生理学 (Environmental Physiology), 神経科学 (Neuroscience)

研究テーマ

○

著書・論文

学術論文(審査論文):

1.	P Chavan, Sachiko Chikahisa, Tetsuya Shiuchi, Noriyuki Shimizu, Junhel Dalanon, Kazuo Okura, Hiroyoshi Sei and Yoshizo Matsuka : Dual orexin receptor antagonist drug suvorexant can help in amelioration of predictable chronic mild stress-induced hyperalgesia, Brain Research Bulletin, Vol.188, 39-46, 2022. (要約) This study aimed to evaluate the involvement of the orexin system in predictable chronic mild stress (PCMS) and the effects of suvorexant, a dual orexin receptor antagonist, on nociceptive behavior in PCMS. Male C57BL/6 J mice were separated into various PCMS groups: a control group with sawdust on the floor of the rearing cage (C), a group with mesh wire on the floor (M), and a group with water just below the mesh wire (W). Activation of lateral hypothalamic orexin neurons was assessed using immunofluorescence. In another experiment, half of the mice in each group were administered an intraperitoneal injection of suvorexant (10 mg/kg), and the remaining mice were injected with the same amount of vehicle (normal saline). Thermal hyperalgesia was examined using tail immersion and hot plate tests, while mechanical hyperalgesia was investigated using the tail pinch test after 21 days of PCMS. Animals subjected to PCMS showed an increased percentage of activated orexin neurons in the lateral hypothalamic region after 21 days. Mice raised in the PCMS environment showed increased pain sensitivity in several pain tests; however, the symptoms were significantly reduced by suvorexant administration. The findings revealed that PCMS activates hypothalamic orexin neuronal activity, and the use of suvorexant can help attenuate PCMS-induced thermal and mechanical hyperalgesia. (徳島大学機関リポジトリ) ● Metadata: 118649 (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.brainresbull.2022.07.011 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35868501 ● Search Scopus @ Elsevier (PMID): 35868501 ● Search Scopus @ Elsevier (DOI): 10.1016/j.brainresbull.2022.07.011 (徳島大学機関リポジトリ: 118649, DOI: 10.1016/j.brainresbull.2022.07.011, PubMed: 35868501)
2.	Tetsuya Shiuchi, Airi Otsuka, Noriyuki Shimizu, Sachiko Chikahisa and Hiroyoshi Sei : Feeding Rhythm-Induced Hypothalamic Agouti-Related Protein Elevation via Glucocorticoids Leads to Insulin Resistance in Skeletal Muscle., International Journal of Molecular Sciences, Vol.22, No.19, 2021. (要約) Circadian phase shifts in peripheral clocks induced by changes in feeding rhythm often result in insulin resistance. However, whether the hypothalamic control system for energy metabolism is involved in the feeding rhythm-related development of insulin resistance is unknown. Here, we show the physiological significance and mechanism of the involvement of the agouti-related protein (AgRP) in evening feeding-associated alterations in insulin sensitivity. Evening feeding during the active dark period increased hypothalamic AgRP expression and skeletal muscle insulin resistance in mice. Inhibiting AgRP expression by administering an antisense oligo or a glucocorticoid receptor antagonist mitigated these effects. AgRP-producing neuron-specific glucocorticoid receptor-knockout (AgRP-GR-KO) mice had normal skeletal muscle insulin sensitivity even under evening feeding schedules. Hepatic vagotomy enhanced AgRP expression in the hypothalamus even during ad-lib feeding in wild-type mice but not in AgRP-GR-KO mice. The findings of this study indicate that feeding in the late active period may affect hypothalamic AgRP expression via glucocorticoids and induce skeletal muscle insulin resistance. (キーワード) Agouti-Related Protein / Animals / エネルギー代謝 (energy metabolism) / Feeding Behavior / Glucocorticoids / 視床下部 (hypothalamus) / インスリン抵抗性 (insulin resistance) / 男性 (male) / Mice / Mice, Inbred C57BL / Muscle, Skeletal / Receptors, Glucocorticoid (徳島大学機関リポジトリ) ● Metadata: 117270 (出版サイトへのリンク) ● Publication site (DOI): 10.3390/ijms221910831 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34639172 ● Summary page in Scopus @ Elsevier: 2-s2.0-85116446550 (徳島大学機関リポジトリ: 117270, DOI: 10.3390/ijms221910831, PubMed: 34639172, Elsevier: Scopus)
3.	Junhel Dalanon, Sachiko Chikahisa, Tetsuya Shiuchi, Noriyuki Shimizu, P Chavan, Yoshitaka Suzuki, Kazuo Okura, Hiroyoshi Sei and Yoshizo Matsuka : Pain sensitivity increases with sleep disturbance under predictable chronic mild stress in mice, Scientific Reports, Vol.11, No.1, 14231, 2021. (徳島大学機関リポジトリ) ● Metadata: 116125 (出版サイトへのリンク) ● Publication site (DOI): 10.1038/s41598-021-93560-7 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-85109362985 (徳島大学機関リポジトリ: 116125, DOI: 10.1038/s41598-021-93560-7, Elsevier: Scopus)
4.	Daisuke Tanioka, Sachiko Chikahisa, Noriyuki Shimizu, Tetsuya Shiuchi, Noriaki Sakai, Seiji Nishino and Hiroyoshi Sei : Intracranial mast cells contribute to the control of social behavior in male mice., Behavioural Brain Research, Vol.403, 113143, 2021. (要約) Mast cells (MCs) exist intracranially and have been reported to affect higher brain functions in rodents. However, the role of MCs in the regulation of emotionality and social behavior is unclear. In the present study, using male mice, we examined the relationship between MCs and social behavior and investigated the underlying mechanisms. Wild-type male mice intraventricularly injected with a degranulator of MCs exhibited a marked increase in a three-chamber sociability test. In addition, removal of MCs in Mast cell-specific Toxin Receptor-mediated Conditional cell Knock out (Mas-TRECK) male mice showed reduced social preference levels in a three-chamber sociability test without other behavioral changes, such as anxiety-like and depression-like behavior. Mas-TRECK male mice also had reduced serotonin content and serotonin receptor expression and increased oxytocin receptor expression in the brain. These results suggested that MCs may contribute to the regulation of social behavior in male mice. This effect may be partially mediated by serotonin derived from MCs in the brain. (徳島大学機関リポジトリ) ● Metadata: 116053 (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.bbr.2021.113143 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33516739 ● Search Scopus @ Elsevier (PMID): 33516739 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbr.2021.113143 (徳島大学機関リポジトリ: 116053, DOI: 10.1016/j.bbr.2021.113143, PubMed: 33516739)
5.	Yoshitsugu Kondoh, Sachiko Chikahisa, Tetsuya Shiuchi, Noriyuki Shimizu, Daisuke Tanioka, Haruo Uguisu and Hiroyoshi Sei : Sleep profile during fasting in PPAR-alpha knockout mice., Physiology & Behavior, Vol.214, 112760, 2020. (要約) Peroxisome proliferator-activated receptor alpha (PPARα) is a transcription factor that belongs to the nuclear receptor family and plays an important role in regulating gene expression associated with lipid metabolism. PPARα promotes hepatic fatty acid oxidation and ketogenesis in response to fasting. Because energy metabolism is known to affect sleep regulation, manipulations that change PPARα are likely to affect sleep and other physiological phenotypes. In this study, we examined the role of PPARα in sleep/wake regulation using PPARα knockout (KO) mice. Sleep, body temperature (BT), locomotor activity, arterial pressure (AP) and heart rate (HR) were recorded in KO mice and wild-type (WT) controls under ad libitum-fed conditions and 24-hour food deprivation (FD). KO and WT mice were identical in basal sleep amount, BT, mean AP and HR, although KO mice showed enhanced sleepiness (enhanced EEG slow-wave activity). In response to FD, KO mice showed a large drop in wakefulness and locomotor activity at the end of the dark phase, whereas WT mice did not. Similarly, AP and HR, which were suppressed by FD, decreased more in KO than in WT mice. Compared to WT mice, KO mice showed a reduced concentration of plasma ketone bodies and decreased mRNA expression of the ketogenic enzyme gene Hmgcs2 in the liver and brain under FD conditions. These results suggest that PPARα and/or lipid metabolism is involved in the maintenance of wakefulness and locomotor activity during fasting in mice. (徳島大学機関リポジトリ) ● Metadata: 114637 (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.physbeh.2019.112760 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31785270 ● Search Scopus @ Elsevier (PMID): 31785270 ● Search Scopus @ Elsevier (DOI): 10.1016/j.physbeh.2019.112760 (徳島大学機関リポジトリ: 114637, DOI: 10.1016/j.physbeh.2019.112760, PubMed: 31785270)
6.	Kazuhiro Muramatsu, Sachiko Chikahisa, Noriyuki Shimizu, Hiroyoshi Sei and Yuichi Inoue : Rotigotine suppresses sleep-related muscle activity augmented by injection of dialysis patients' sera in a mouse model of restless legs syndrome., Scientific Reports, Vol.9, No.1, 2019. (要約) Idiopathic restless legs syndrome (RLS) has a genetic basis wherein BTBD9 is associated with a higher risk of RLS. Hemodialysis patients also exhibit higher rates of RLS compared with the healthy population. However, little is known about the relationship of BTBD9 and end-stage renal disease to RLS pathophysiology. Here we evaluated sleep and leg muscle activity of Btbd9 mutant (MT) mice after administration of serum from patients with either idiopathic or RLS due to end-stage renal disease (renal RLS) and investigated the efficacy of treatment with the dopamine agonist rotigotine. At baseline, the amount of rapid eye movement (REM) sleep was decreased and leg muscle activity during non-REM (NREM) sleep was increased in MT mice compared to wild-type (WT) mice. Wake-promoting effects of rotigotine were attenuated by injection of serum from RLS patients in both WT and MT mice. Leg muscle activity during NREM sleep was increased only in MT mice injected with serum from RLS patients of ideiopatic and renal RLS. Subsequent treatment with rotigotine ameliorated this altered leg muscle activity. Together these results support previous reports showing a relationship between the Btbd9/dopamine system and RLS, and elucidate in part the pathophysiology of RLS. (徳島大学機関リポジトリ) ● Metadata: 114948 (出版サイトへのリンク) ● Publication site (DOI): 10.1038/s41598-019-52735-z (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31704978 ● Search Scopus @ Elsevier (PMID): 31704978 ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-019-52735-z (徳島大学機関リポジトリ: 114948, DOI: 10.1038/s41598-019-52735-z, PubMed: 31704978)
7.	Tetsuya Shiuchi, Takuya Masuda, Noriyuki Shimizu, Sachiko Chikahisa and Hiroyoshi Sei : Dopamine stimulation of the septum enhances exercise efficiency during complicated treadmill running in mice., The Journal of Physiological Sciences, Vol.69, No.6, 1019-1028, 2019. (要約) We aimed to identify the neurotransmitters and brain regions involved in exercise efficiency in mice during continuous complicated exercises. Male C57BL/6J mice practiced treadmill running with intermittent obstacles on a treadmill for 8 days. Oxygen uptake (VO (出版サイトへのリンク) ● Publication site (DOI): 10.1007/s12576-019-00722-4 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31664642 ● Search Scopus @ Elsevier (PMID): 31664642 ● Search Scopus @ Elsevier (DOI): 10.1007/s12576-019-00722-4 (DOI: 10.1007/s12576-019-00722-4, PubMed: 31664642)
8.	Airi Otsuka, Tetsuya Shiuchi, Sachiko Chikahisa, Noriyuki Shimizu and Hiroyoshi Sei : Sufficient intake of high-fat food attenuates stress-induced social avoidance behavior., Life Sciences, Vol.219, 219-230, 2019. (要約) Mice subjected to SDS ate either a high-fat or normal chow diet for 10 days. Behavioral tests were conducted following the completion of the SDS paradigm. The hypothalamus, liver, and blood were examined post-mortem. (キーワード) Animals / Avoidance Learning / 脳 (brain) / コレステロール (cholesterol) / Corticosterone / Diet, High-Fat / Liver / Male / Mice / Mice, Inbred C57BL / Real-Time Polymerase Chain Reaction / 社会的行動 (social behavior) / Stress, Psychological (徳島大学機関リポジトリ) ● Metadata: 113355 (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.lfs.2019.01.012 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30653972 ● Summary page in Scopus @ Elsevier: 2-s2.0-85060171119 (徳島大学機関リポジトリ: 113355, DOI: 10.1016/j.lfs.2019.01.012, PubMed: 30653972, Elsevier: Scopus)
9.	Sachiko Chikahisa, Daiki Chida, Tetsuya Shiuchi, Saki Harada, Noriyuki Shimizu, Airi Otsuka, Daisuke Tanioka and Hiroyoshi Sei : Enhancement of fear learning in PPAR knockout mice., Behavioural Brain Research, Vol.359, 664--670, 2019. (要約) Peroxisome proliferator-activated receptor alpha (PPAR) is a member of the nuclear receptor superfamily and regulates fatty acid oxidation. Although PPAR is expressed not only in the peripheral tissues but also in the brain, its role in higher brain function is unclear. In this study, we investigated the role of PPAR in the control of behavior, including memory/learning and mood change, using PPAR knockout (KO) mice. A significant difference between wild-type (WT) and KO mice was seen in the passive avoidance test, demonstrating that KO mice showed enhanced fear leaning. In the amygdala of KO mice, the levels of dopamine and its metabolites were increased, and the mRNA expression of dopamine degrading enzyme was decreased. When dopamine D1 receptor antagonist was administered, the enhanced fear learning observed in KO mice was attenuated. These results suggest that PPAR is involved in the regulation of emotional memory via the dopamine pathway in the amygdala. (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.bbr.2018.09.020 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30278189 ● Search Scopus @ Elsevier (PMID): 30278189 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbr.2018.09.020 (DOI: 10.1016/j.bbr.2018.09.020, PubMed: 30278189)
10.	Sachiko Chikahisa, Saki Harada, Noriyuki Shimizu, Tetsuya Shiuchi, Airi Otsuka, Seiji Nishino and Hiroyoshi Sei : Mast cell involvement in glucose tolerance impairment caused by chronic mild stress with sleep disturbance., Scientific Reports, Vol.7, No.1, 2017. (要約) We have developed a chronic mild stress (MS) mouse model by simply rearing mice on a wire net for 3 weeks and investigated the effects of MS on glucose homeostasis and sleep. MS mice showed impaired glucose tolerance and disturbed sleep. One-week treatment with a histamine H1 receptor antagonist (H1RA) ameliorated the glucose intolerance and improved sleep quality in MS mice. MS mice showed an increased number of mast cells in both adipose tissue and the brain. Inhibition of mast cell function ameliorated the impairment in both glucose tolerance and sleep. Together, these findings indicate that mast cells may represent an important pathophysiological mediator in sleep and energy homeostasis. (徳島大学機関リポジトリ) ● Metadata: 112403 (出版サイトへのリンク) ● Publication site (DOI): 10.1038/s41598-017-14162-w (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29057915 ● Summary page in Scopus @ Elsevier: 2-s2.0-85032017812 (徳島大学機関リポジトリ: 112403, DOI: 10.1038/s41598-017-14162-w, PubMed: 29057915, Elsevier: Scopus)
11.	Kanna Oura, Airi Otsuka, Tetsuya Shiuchi, Sachiko Chikahisa, Noriyuki Shimizu and Hiroyoshi Sei : Late feeding in the active period decreases slow-wave activity., Life Sciences, Vol.160, 18-26, 2016. (要約) Sleep and feeding behaviors closely interact to maintain energy homeostasis. While it is known that sleep disorders can lead to various metabolic issues such as insulin resistance, the mechanism for this effect is poorly understood. We thus investigated whether different feeding rhythms during the active period affect sleep-wake regulation. For 2weeks, mice were randomly assigned to 1 of 3 feeding schedules as follows: free access to lab chow during the active period (ZT12-24, Ad-lib group), free access to lab chow during the first half of the active period (ZT12-18; Morning group), or free access to lab chow during the second half of the active period (ZT18-24, Evening group). Food intake, body weight, body temperature, locomotor activity, and sleep were evaluated. The hypothalamus and cerebral cortex were examined post-mortem. No alterations in food intake or body weight were observed among the 3 groups. The Evening group showed lower slow-wave activity (SWA) than the other 2 groups, in addition to higher expression of orexin mRNA in the hypothalamus and higher concentrations of dopamine and its metabolites in the cerebral cortex. AMPK phosphorylation was increased in the hypothalamus of mice in the Evening group; however, AMPK inhibition had no effect on SWA. We concluded that late feeding reduces SWA in NREM sleep via a mechanism that involves orexin-mediated arousal in the hypothalamus and elevated monoamines in the cerebral cortex. These data have important implications for the relationship between sleep-wake disturbances and metabolic disorders. (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.lfs.2016.07.005 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27436545 ● Search Scopus @ Elsevier (PMID): 27436545 ● Search Scopus @ Elsevier (DOI): 10.1016/j.lfs.2016.07.005 (DOI: 10.1016/j.lfs.2016.07.005, PubMed: 27436545)
12.	Airi Otsuka, Tetsuya Shiuchi, Sachiko Chikahisa, Noriyuki Shimizu and Hiroyoshi Sei : Voluntary exercise and increased food intake after mild chronic stress improve social avoidance behavior in mice., Physiology & Behavior, Vol.151, 264-271, 2015. (要約) It is well-established that exercise can influence psychological conditions, cognitive function, and energy metabolism in peripheral tissues including the skeletal muscle. However, it is not clear whether exercise can influence social interaction with others and alleviate defeat stress. This study investigated the effect of voluntary wheel running on impaired social interaction induced by chronic social defeat stress (SDS) using the resident-intruder social defeat model. Mice were divided into three groups: control, stress alone, and stress+exercise. SDS was performed by exposing C57BL/6 mice to retired ICR mice for 2.5 min. The C57BL/6 mice were continuously defeated by these resident (aggressor) mice and, following 5 days of SDS, experienced 2 days of rest with no SDS. Mice in the stress+exercise group were allowed to voluntarily run on a wheel for 2h after every SDS exposure. Two weeks later, compared to the control group, the stress group showed a higher ratio of time spent in the corner zone of a social interaction paradigm even though SDS did not elicit depressive- and anxiety-like behaviors. We also observed that voluntary exercise, which did not affect muscle weight and gene expression, decreased social avoidance behavior of stressed mice without clear changes in brain monoamine levels. Interestingly, food intake in the stress+exercise group was the greatest among the three groups. To test the effect of the exercise-induced increase in food intake on social behavior, we set up a pair-fed group where food intake was restricted. We then compared these mice to mice in the stress alone group. We found that the ratio of time spent in the corner zone of the social interaction test was not different between ad libitum- and pair-fed groups, although pair-fed mice spent more time in the corner zone when an aggressor mouse was present than when it was absent. In addition, pair-feeding did not show exercise-induced reductions of adrenal gland weight and enhanced the loss of body fat. Our findings indicate that voluntary exercise reduces social avoidance behavior induced by SDS. Further, we determined that SDS and exercise-induced increases in food intake partially influence energy metabolism and social avoidance behavior. (キーワード) Animals / Anxiety / Brain / Depression / Disease Models, Animal / Dominance-Subordination / Dopamine / Eating / Gene Expression / Male / Mice, Inbred C57BL / Mice, Inbred ICR / Motor Activity / Running / Serotonin / Social Behavior / Stress, Psychological / Volition (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.physbeh.2015.07.024 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26232088 ● Summary page in Scopus @ Elsevier: 2-s2.0-84939498491 (DOI: 10.1016/j.physbeh.2015.07.024, PubMed: 26232088, Elsevier: Scopus)
13.	Noriyuki Shimizu, Sachiko Chikahisa, Yuina Nishi, Saki Harada, Yohei Iwaki, Hiroaki Fujihara, Kazuyoshi Kitaoka, Tetsuya Shiuchi and Hiroyoshi Sei : Maternal Dietary Restriction Alters Offspring's Sleep Homeostasis., PLoS ONE, Vol.8, No.5, e64263, 2013. (要約) Nutritional state in the gestation period influences fetal growth and development. We hypothesized that undernutrition during gestation would affect offspring sleep architecture and/or homeostasis. Pregnant female mice were assigned to either control (fed ad libitum; AD) or 50% dietary restriction (DR) groups from gestation day 12 to parturition. After parturition, dams were fed AD chow. After weaning, the pups were also fed AD into adulthood. At adulthood (aged 8-9 weeks), we carried out sleep recordings. Although offspring mice displayed a significantly reduced body weight at birth, their weights recovered three days after birth. Enhancement of electroencephalogram (EEG) slow wave activity (SWA) during non-rapid eye movement (NREM) sleep was observed in the DR mice over a 24-hour period without changing the diurnal pattern or amounts of wake, NREM, or rapid eye movement (REM) sleep. In addition, DR mice also displayed an enhancement of EEG-SWA rebound after a 6-hour sleep deprivation and a higher threshold for waking in the face of external stimuli. DR adult offspring mice exhibited small but significant increases in the expression of hypothalamic peroxisome proliferator-activated receptor (Ppar) and brain-specific carnitine palmitoyltransferase 1 (Cpt1c) mRNA, two genes involved in lipid metabolism. Undernutrition during pregnancy may influence sleep homeostasis, with offspring exhibiting greater sleep pressure. (キーワード) Animals / Body Weight / Caloric Restriction / Carnitine O-Palmitoyltransferase / Diet / Electroencephalography / Female / Gene Expression / Homeostasis / Hypothalamus / Mice / PPAR alpha / Pregnancy / Prenatal Exposure Delayed Effects / Sleep / Sleep, REM / Wakefulness (徳島大学機関リポジトリ) ● Metadata: 106073 (出版サイトへのリンク) ● Publication site (DOI): 10.1371/journal.pone.0064263 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23741310 ● Summary page in Scopus @ Elsevier: 2-s2.0-84878536785 (徳島大学機関リポジトリ: 106073, DOI: 10.1371/journal.pone.0064263, PubMed: 23741310, Elsevier: Scopus)

国際会議:

1.	Junhel Dalanon, LM Diano, I Fernandez, CM Estalani, MP Te, C Mana-ay, E Lagra, Sachiko Chikahisa, Noriyuki Shimizu, Rozzano De Castro Locsin and Yoshizo Matsuka : Occupational stress and sleep quality of dentists in the Philippines, International Association for Human Caring, Bergen, Norway, Jun. 2021.
2.	Sachiko Chikahisa, Kazuhiro Muramatsu, Noriyuki Shimizu, Hiroyoshi Sei and Yuichi Inoue : Sleep-related muscle activity is augmented by administration of serum from RLS patients in Btbd9 mutant mice., International RLS Study Group Meeting, Philadelphia, Aug. 2020.
3.	Junhel Dalanon, Sachiko Chikahisa, Noriyuki Shimizu, Arief Waskitho, Hiroyoshi Sei and Yoshizo Matsuka : Orofacial thermal hyperalgesia in predictable chronic mild stress, Asian Academy of Orofacial Pain and Temporomandibular Disorders, Manila, Oct. 2019.
4.	Junhel Dalanon, Sachiko Chikahisa, S Afroz, Takuma Iwasa, Arief Waskitho, Noriyuki Shimizu, Hiroyoshi Sei and Yoshizo Matsuka : Chronic mild stress exacerbates sleep quality and pain threshold, Philippine Dental Association, Manila, May 2019.
5.	Tetsuya Shiuchi, Takuya Masuda, Noriyuki Shimizu, Sachiko Chikahisa and Hiroyoshi Sei : Role of dopaminergic function in septum on exercise efficiency, FAOSP2019, Mar. 2019.
6.	Sachiko Chikahisa, Tetsuya Shiuchi, Tanioka Daisuke, Noriyuki Shimizu, Otsuka Airi and Hiroyoshi Sei : Chronic mild stress increases aggressive behavior in mice, FAOPS 2019, Kobe, Mar. 2018.
7.	Sachiko Chikahisa, Harada Saki, Noriyuki Shimizu, Tetsuya Shiuchi, Nishino Seiji and Hiroyoshi Sei : Sleep loss induces diabetes in mice model, Neuroscience 2016, San Diego, USA, Nov. 2016.
8.	Noriyuki Shimizu, Yu Yoshioka, Sachiko Chikahisa, Yuki Kito, Tetsuya Shiuchi and Hiroyoshi Sei : Short time running exercise enhances sleep pressure in mice., Neuroscience 2016, San Diego, USA, Nov. 2016.
9.	Sachiko Chikahisa, Noriyuki Shimizu, Tetsuya Shiuchi and Hiroyoshi Sei : Contribution of PPARa and Ketone Body to Sleep Homeostasis in Mice, The FASEB journal, Boston, Apr. 2015.
10.	Sachiko Chikahisa, Saki Harada, Noriyuki Shimizu, Tetsuya Shiuchi and Hiroyoshi Sei : Sleep quality affects glucose homeostasis in a mouse model, Homeodynamics in Clocks, Sleep and Metabolism, Tokyo Translational Therapeutics Meeting, Sep. 2014.
11.	Noriyuki Shimizu, Masahiro Oura, Sachiko Chikahisa and Hiroyoshi Sei : EEG delta power in NREM sleep is enhanced by refeeding after 24-hr fasting in time-dependent manner, 36th International Union of Physiological Sciences, Kyoto, Aug. 2009.

国内講演発表:

1.	Sachiko Chikahisa, Dalanon Junhel, Chavan Parimal Ravindra, Tetsuya Shiuchi, Noriyuki Shimizu, Kazuo Okura, Yoshitaka Suzuki, Yoshizo Matsuka and Hiroyoshi Sei : Effects of mild chronic stress on sleep and pain thresholds in mice, 日本生理学会, Mar. 2024.
2.	近久幸子, Junhel Dalanon, Parimal Chavan, 志内哲也, 清水紀之, 大倉一夫, 鈴木善貴, 松香芳三, 勢井宏義 : 睡眠障害を伴う軽度慢性ストレスは痛覚過敏を引き起こす, 日本睡眠学会, 2023年9月.
3.	Noriyuki Shimizu, Sachiko Chikahisa, Tetsuya Shiuchi and Hiroyoshi Sei : Paternal psychological stress exposure prior to mating may cause an impairment of emotional behavior in the next generation via glucocorticoid system, 第46回日本神経科学大会, Aug. 2023.
4.	Noriyuki Shimizu, Sachiko Chikahisa, Tetsuya Shiuchi and Hiroyoshi Sei : Exercise training of paternal mice subjected to continuous psychological stress prior to mating may modify changes in their offsprings emotional behaviors, 日本生理学会第100回記念大会, Mar. 2023.
5.	Noriyuki Shimizu, Sachiko Chikahisa, Tetsuya Shiuchi and Hiroyoshi Sei : Paternal exposure to psychological stress before mating may influence a formation of emotional behavior in next generation via effect of glucocorticoid, 第45回日本分子生物学会年会, Dec. 2022.
6.	Noriyuki Shimizu, Sachiko Chikahisa, Tetsuya Shiuchi and Hiroyoshi Sei : Glucocorticoid receptor antagonist (RU-486) prevents a defect emotional behavior observed in offspring derived from paternal mouse subjected psychological stress, NEURO2022 (456532), Jul. 2022.
7.	志内哲也, 増田拓也, 清水紀之, 近久幸子, 勢井宏義 : 複雑な運動中の運動効率に及ぼすドーパミン作用, 第74回日本体力医学会, 2019年9月.
8.	志内哲也, 清水紀之, 大塚愛理, 近久幸子, 勢井宏義 : 妊娠期における摂食リズムの違いが仔における行動に及ぼす影響, 第6回時間栄養科学研究会, 2019年8月.
9.	志内哲也, 増田拓也, 清水紀之, 近久幸子, 勢井宏義 : 運動効率を高める中隔核でのドパミン作用, 第73回日本体力医学会, 2018年9月.
10.	Noriyuki Shimizu, Sachiko Chikahisa, Tetsuya Shiuchi, Airi Otsuka, Daisuke Tanioka and Hiroyoshi Sei : Paternal psychological stress just before mating influences a formation of emotional behavior in next generation offspring mice, The 41st Annual Meeting of the Japan Neuroscience Society, Jul. 2018.
11.	Daisuke Tanioka, Sachiko Chikahisa, Noriyuki Shimizu, Tetsuya Shiuchi, Airi Otsuka and Hiroyoshi Sei : Social behavior is affected by mast cells activity in mice, The 41st Annual Meeting of the Japan Neuroscience Society, Jul. 2018.
12.	増田拓也, Tetsuya Shiuchi, Sachiko Chikahisa, Noriyuki Shimizu and Hiroyoshi Sei : 中隔核でのドパミン作用と運動効率の関係, 第41回日本神経科学学会大会, Jul. 2018.
13.	谷岡大輔, 大上路生, 近久幸子, 清水紀之, 志内哲也, 大塚愛理, 勢井宏義 : 性腺の摘出は睡眠を変化させる, 日本睡眠学会第43回定期学術集会, 2018年7月.
14.	清水紀之, 志内哲也, 近久幸子, 大塚愛理, 勢井宏義 : 視床下部Agouti-related protein (AgRP)産生神経が絶食や再摂餌による睡眠調節に果たす役割, 日本睡眠学会第43回定期学術集会, 2018年7月.
15.	Noriyuki Shimizu, Sachiko Chikahisa, Tetsuya Shiuchi, Daisuke Tanioka, Airi Otsuka and Hiroyoshi Sei : Paternal psychological stress causes an impairment of emotional behavior in offspring mice, The 95th Annual Meeting of the Physiological Society of Japan, Mar. 2018.
16.	Daisuke Tanioka, Sachiko Chikahisa, Noriyuki Shimizu, Tetsuya Shiuchi, Airi Otsuka and Hiroyoshi Sei : The role of brain mast cells in mood and behavior, The 95th Annual Meeting of the Physiological Society of Japan, Mar. 2018.
17.	清水紀之, 近久幸子, 志内哲也, 谷岡大輔, 大塚愛理, 勢井宏義 : 拘束ストレスを受けた雄マウスを父親とする次世代仔マウスは情動障害を有する, 第13回環境生理学プレコングレス, 2018年3月.
18.	志内哲也, 大塚愛理, 近久幸子, 清水紀之, 勢井宏義 : グルココルチコイドにより視床下部AgRPが発現増強する臓器連関メカニズムの解明, 2017年度生命科学系学会合同年次大会(ConBio2017), 2017年12月.
19.	谷岡大輔, 近久幸子, 原田紗希, 清水紀之, 志内哲也, 大塚愛理, 勢井宏義 : 慢性ストレスにおける睡眠・代謝異常と肥満細胞の関係, 第69回日本生理学会中国・四国地方会, 2017年10月.
20.	近久幸子, 清水紀之, 大塚愛理, 志内哲也, 勢井宏義 : 睡眠不足モデルマウスにおける情動行動と運動学習能の変化, 日本睡眠学会第42回定期学術集会, 2017年6月.
21.	近久幸子, 清水紀之, 大塚愛理, 志内哲也, 西野精治, 勢井宏義 : 脳内肥満細胞の睡眠覚醒調節における役割, 第12回環境生理プレコングレス, 2017年3月.
22.	Tetsuya Shiuchi, Noriyuki Shimizu, Airi Otsuka, Sachiko Chikahisa and Hiroyoshi Sei : Alteration of behavior in offspring born from mother lived in the different feeding rhythm during pregnancy, The 94th Annual Meeting of the Physiological Society of Japan, Mar. 2017.
23.	Noriyuki Shimizu, Yu Yoshioka, Takafumi Misaki, Yuki Kito, Sachiko Chikahisa, Tetsuya Shiuchi and Hiroyoshi Sei : Sleep depth is affected by acute running exercise in mice., The 39th Annual Meeting of the Japan Neuroscience Society, Jul. 2016.
24.	近久幸子, 清水紀之, 志内哲也, 西野精治, 勢井宏義 : 睡眠障害モデルマウスの糖代謝異常におけるマストセルの関与, 日本睡眠学会第41回定期学術集会, 2016年7月.
25.	清水紀之, 吉岡由布, 三崎貴文, 木藤有紀, 近久幸子, 志内哲也, 勢井宏義 : トレッドミルによる一過性の短時間運動に伴う睡眠への影響とケトン体代謝との関連性, 日本睡眠学会第41回定期学術集会, 2016年7月.
26.	Noriyuki Shimizu, Yu Yoshioka, Takafumi Misaki, Sachiko Chikahisa, Tetsuya Shiuchi and Hiroyoshi Sei : Acute running exercise enhances sleep in mice, The 93rd Annual Meeting of the Physiological Society of Japan, Mar. 2016.

その他・研究会:

1.	Dalanon Junhel, Sachiko Chikahisa, Noriyuki Shimizu, Waskitho Arief, Chavan Parimal, Lakshmi Swarna, Hiroyoshi Sei, Masamitsu Ohshima and Yoshizo Matsuka : The effect of predictable chronic mild stress on sleep quality and nociception, 徳島大学脳科学クラスターミニリトリート, Feb. 2020.

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

心理的ストレスを受けた父親により運命づけられる次世代の情動機能と機序解明 (研究課題/領域番号: 20K16859 )
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助教 : 清水紀之

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科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

助教 : 清水 紀之

研究活動

専門分野

研究テーマ

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学術論文(審査論文):

国際会議:

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科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

助教 : 清水紀之