type: misc

label: url

en: ゲノム編集技術による遺伝子改変家畜の作出

ja: ゲノム編集技術による遺伝子改変家畜の作出

en: Journal of Mammalian Ova Research

ja: Journal of Mammalian Ova Research


en: Current status of the application of gene editing in pigs.

en: Genetically modified animals, especially rodents, are widely used in biomedical research. However, non-rodent models are required for efficient translational medicine and preclinical studies. Owing to the similarity in the physiological traits of pigs and humans, genetically modified pigs may be a valuable resource for biomedical research. Somatic cell nuclear transfer (SCNT) using genetically modified somatic cells has been the primary method for the generation of genetically modified pigs. However, site-specific gene modification in porcine cells is inefficient and requires laborious and time-consuming processes. Recent improvements in gene-editing systems, such as zinc finger nucleases, transcription activator-like effector nucleases, and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (CRISPR/Cas) system, represent major advances. The efficient introduction of site-specific modifications into cells via gene editors dramatically reduces the effort and time required to generate genetically modified pigs. Furthermore, gene editors enable direct gene modification during embryogenesis, bypassing the SCNT procedure. The application of gene editors has progressively expanded, and a range of strategies is now available for porcine gene engineering. This review provides an overview of approaches for the generation of genetically modified pigs using gene editors, and highlights the current trends, as well as the limitations, of gene editing in pigs.

en: The Journal of Reproduction and Development


en: Recovery options of reproductive function of cancer patients due to transplantation of cryopreserved ovarian tissue.

en: Genes and Cells

ja: Genes and Cells


en: Studying spermatogenesis by using in vivo and in vitro models: Advantages and disadvantages of these models for practical use.

en: Journal of Veterinary Science & Technology


en: Effects of large follicle aspiration on superovulatory response of beef cows.

en: Archives Animal Breeding

ja: Archives Animal Breeding


en: In vitro growth of bovine oocytes.

ja: In vitro growth of bovine oocytes.

en: 4th Asian Symposium on Animal Biotechnology, Proceedings


en: 卵母細胞の体外発育 ―発育途上卵母細胞からの子ウシ生産の可能性―

ja: 卵母細胞の体外発育 ―発育途上卵母細胞からの子ウシ生産の可能性―

en: 畜産の研究

ja: 畜産の研究


en: 平成29年度 徳島大学総合教育センターアドミッション部門 報告書

ja: 平成29年度 徳島大学総合教育センターアドミッション部門 報告書

en: 平成29年度 徳島大学総合教育センターアドミッション部門 報告書

ja: 平成29年度 徳島大学総合教育センターアドミッション部門 報告書

misc_type: others