ishimaruyoshiyasu/published_papers/54122251 Shintaro Inoue Ayane Shimamura Kenichiro Koshiyama Sayuri Tomonari Taiyo Takada Yoshiyasu Ishimaru Katsuyuki Miyawaki Akira Takahashi Takeshi Nikawa Sumihare Noji Takahito Watanabe Taro Mito Gene misexpression system for functional studies of genes involved in cuticle development in the cricket Gryllus bimaculatus. The insect epidermis possesses the potential to generate remarkable diversity in exoskeletal cuticle traits, including colour, thickness and mechanical properties. Genetic manipulation is essential for investigating the molecular mechanisms; however, spatiotemporally controlled gene overexpression remains technically challenging despite the widespread use of loss-of-function approaches in many insects. In this study, we established an epidermal gene misexpression system in the model hemimetabolous insect Gryllus bimaculatus by inserting a gene expression cassette into the evolutionarily conserved yellow gene, which is involved in melanin synthesis, using genome editing. Insertion of the EGFP expression cassette into yellow gene resulted in fluorescence in the epidermis of pre-hatching embryos and post-moult individuals across developmental stages, corresponding to melanin pigmentation. Using this system, we induced the misexpression of Arylalkylamine N-acetyltransferase (aaNAT), an enzyme involved in the synthesis of N-acetyldopamine (NADA)-sclerotin, which serves as both a white pigment and a crosslinker in the cuticle. Compared to the background strain, the aaNAT misexpression strain exhibited a brighter body coloration, indicating that Gryllus aaNAT has the function of producing white NADA sclerotin and suppressing melanin pigment production. Wing thickness exhibited no substantial alteration, whereas a significant reduction in puncture resistance was observed. Collectively, these results suggest that an imbalance in the molecular components essential for cuticular crosslinking leads to alterations in the mechanical properties of the cuticle. This system serves as a gain-of-function analysis tool for cuticle research and has the potential to be applicable across a wide range of insect species, thereby helping to elucidate cuticle diversity. Insect molecular biology null null 20260517 10.1111/imb.70044 42144849 ishimaruyoshiyasu/published_papers/49343919 Kohei Kawamoto Yoshiyasu Ishimaru Sayuri Tomonari Takahito Watanabe Sumihare Noji Taro Mito Myoglianin is a crucial factor for the transition to the juvenile hormone-dependent phase during hemimetabolous nymphal development Elsevier BV Insect Biochemistry and Molecular Biology 0965-1748 178 104274 104274 20250300 10.1016/j.ibmb.2025.104274 ishimaruyoshiyasu/published_papers/48681217 Shintaro Inoue Kai Fujie Taiki Hamaguchi Yoshiyasu Ishimaru Katsuyuki Miyawaki Akira Takahashi Takeshi Nikawa Sumihare Noji Takahito Watanabe Taro Mito Lineage-specific duplication and functional diversification of DOPA-decarboxylase genes in the Gryllidae family, as revealed in Gryllus bimaculatus. The DOPA-decarboxylase (DDC) gene is crucial for dopamine synthesis and influences various biological functions in insects, including body coloration, behavior, learning, and sleep. However, its evolutionary impact remains largely unexplored. This study reports on the tandem duplication of two bona fide ddc genes (ddc1 and ddc2) in the Gryllidae cricket family. We herein investigated the function of ddc1 and ddc2 using Gryllus bimaculatus (Gb) as a model. Our results revealed that Gb'ddc1 was expressed systemically, with its expression being higher immediately after molting compared to the stage following melanin pigmentation. In homozygous knockout mutants of Gb'ddc1, generated via CRISPR/Cas9, reduced body color pigmentation and had translucent cuticles, decreased dopamine levels, and over-accumulated DOPA. These mutants died shortly after hatching, likely due to cuticle defects, underscoring the essential role of dopamine, produced by Gb'ddc1, in melanin synthesis. Conversely, Gb'ddc2 expression was confined to the ovary and was not up-regulated after molting. Homozygous knockout mutants of Gb'ddc2 exhibited no body color defects, whereas hatchability and embryonic development rates were significantly reduced. Interestingly, dopamine levels in the ovaries were significantly elevated in Gb'ddc2 mutants. This suggests that normal ovarian dopamine levels, modulated by Gb'ddc2, are vital for fertility maintenance. The function of Gb'ddc2 differs from that of typical ddc, indicating neofunctionalization through evolutionary duplication. Overall, Gb'ddc1 and Gb'ddc2 have distinct functions, and precise regulation of ovarian dopamine levels using these two ddc genes may have enhanced cricket fertility. Insect biochemistry and molecular biology 104246 104246 20250200 10.1016/j.ibmb.2024.104246 39653316 ishimaruyoshiyasu/published_papers/43106098 Takahisa Yamashita Takahiro Ohde Taro Nakamura Yoshiyasu Ishimaru Takahito Watanabe Sayuri Tomonari Yuki Nakamura Sumihare Noji Taro Mito Involvement of the <i>scalloped</i> gene in morphogenesis of the wing margin via regulating cell growth in a hemimetabolous insect <i>Gryllus bimaculatus</i> Wiley Development, Growth &amp; Differentiation 1440-169X null null 20230600 10.1111/dgd.12869 ishimaruyoshiyasu/published_papers/42650355 Shintaro Inoue Takahito Watanabe Taiki Hamaguchi Yoshiyasu Ishimaru Katsuyuki Miyawaki Takeshi Nikawa Akira Takahashi Sumihare Noji Taro Mito Combinatorial expression of ebony and tan generates body color variation from nymph through adult stages in the cricket, Gryllus bimaculatus. Insect body colors and patterns change markedly during development in some species as they adapt to their surroundings. The contribution of melanin and sclerotin pigments, both of which are synthesized from dopamine, to cuticle tanning has been well studied. Nevertheless, little is known about how insects alter their body color patterns. To investigate this mechanism, the cricket Gryllus bimaculatus, whose body color patterns change during postembryonic development, was used as a model in this study. We focused on the ebony and tan genes, which encode enzymes that catalyze the synthesis and degradation, respectively, of the precursor of yellow sclerotin N-β-alanyl dopamine (NBAD). Expression of the G. bimaculatus (Gb) ebony and tan transcripts tended to be elevated just after hatching and the molting period. We found that dynamic alterations in the combined expression levels of Gb'ebony and Gb'tan correlated with the body color transition from the nymphal stages to the adult. The body color of Gb'ebony knockout mutants generated by CRISPR/Cas9 systemically darkened. Meanwhile, Gb'tan knockout mutants displayed a yellow color in certain areas and stages. The phenotypes of the Gb'ebony and Gb'tan mutants probably result from an over-production of melanin and yellow sclerotin NBAD, respectively. Overall, stage-specific body color patterns in the postembryonic stages of the cricket are governed by the combinatorial expression of Gb'ebony and Gb'tan. Our findings provide insights into the mechanism by which insects evolve adaptive body coloration at each developmental stage. PloS one 18 5 e0285934 null 20230000 10.1371/journal.pone.0285934 37200362 ishimaruyoshiyasu/published_papers/36747866 Yuki Nakamura Sayuri Tomonari Kohei Kawamoto Takahisa Yamashita Takahito Watanabe Yoshiyasu Ishimaru Sumihare Noji Taro Mito Evolutionarily conserved function of the even-skipped ortholog in insects revealed by gene knock-out analyses in Gryllus bimaculatus Elsevier BV Developmental Biology 0012-1606 485 1 8 20220500 10.1016/j.ydbio.2022.02.005 ishimaruyoshiyasu/published_papers/35195229 Tetsuya Bando Misa Okumura Yuki Bando Marou Hagiwara Yoshimasa Hamada Yoshiyasu Ishimaru Taro Mito Eri Kawaguchi Takeshi Inoue Kiyokazu Agata Sumihare Noji Hideyo Ohuchi Toll signalling promotes blastema cell proliferation during cricket leg regeneration via insect macrophages ABSTRACT Hemimetabolous insects, such as the two-spotted cricket Gryllus bimaculatus, can recover lost tissues, in contrast to the limited regenerative abilities of human tissues. Following cricket leg amputation, the wound surface is covered by the wound epidermis, and plasmatocytes, which are insect macrophages, accumulate in the wound region. Here, we studied the function of Toll-related molecules identified by comparative RNA sequencing during leg regeneration. Of the 11 Toll genes in the Gryllus genome, expression of Toll2-1, Toll2-2 and Toll2-5 was upregulated during regeneration. RNA interference (RNAi) of Toll, Toll2-1, Toll2-2, Toll2-3 or Toll2-4 produced regeneration defects in more than 50% of crickets. RNAi of Toll2-2 led to a decrease in the ratio of S- and M-phase cells, reduced expression of JAK/STAT signalling genes, and reduced accumulation of plasmatocytes in the blastema. Depletion of plasmatocytes in crickets using clodronate also produced regeneration defects, as well as fewer proliferating cells in the regenerating legs. Plasmatocyte depletion also downregulated the expression of Toll and JAK/STAT signalling genes in the regenerating legs. These results suggest that Spz-Toll-related signalling in plasmatocytes promotes leg regeneration through blastema cell proliferation by regulating the Upd-JAK/STAT signalling pathway. The Company of Biologists Development 1477-9129 149 8 null null 20220400 10.1242/dev.199916 34622924 ishimaruyoshiyasu/published_papers/39801421 Taro Mito Yoshiyasu Ishimaru Takahito Watanabe Taro Nakamura Guillem Ylla Sumihare Noji Cassandra G Extavour Cricket: The third domesticated insect. Many researchers are using crickets to conduct research on various topics related to development and regeneration in addition to brain function, behavior, and biological clocks, using advanced functional and perturbational technologies such as genome editing. Recently, crickets have also been attracting attention as a food source for the next generation of humans. In addition, crickets are increasingly being used as disease models and biological factories for pharmaceuticals. Cricket research has thus evolved over the last century from use primarily in highly important basic research, to use in a variety of applications and practical uses. These insects are now a state-of-the-art model animal that can be obtained and maintained in large quantities at low cost. We therefore suggest that crickets are useful as a third domesticated insect for scientific research, after honeybees and silkworms, contributing to the achievement of global sustainable development goals. Current topics in developmental biology 147 291 306 20220000 10.1016/bs.ctdb.2022.02.003 35337452 ishimaruyoshiyasu/published_papers/39801401 Guillem Ylla Taro Nakamura Takehiko Itoh Rei Kajitani Atsushi Toyoda Sayuri Tomonari Tetsuya Bando Yoshiyasu Ishimaru Takahito Watanabe Masao Fuketa Yuji Matsuoka Austen A Barnett Sumihare Noji Taro Mito Cassandra G Extavour Insights into the genomic evolution of insects from cricket genomes. Most of our knowledge of insect genomes comes from Holometabolous species, which undergo complete metamorphosis and have genomes typically under 2 Gb with little signs of DNA methylation. In contrast, Hemimetabolous insects undergo the presumed ancestral process of incomplete metamorphosis, and have larger genomes with high levels of DNA methylation. Hemimetabolous species from the Orthopteran order (grasshoppers and crickets) have some of the largest known insect genomes. What drives the evolution of these unusual insect genome sizes, remains unknown. Here we report the sequencing, assembly and annotation of the 1.66-Gb genome of the Mediterranean field cricket Gryllus bimaculatus, and the annotation of the 1.60-Gb genome of the Hawaiian cricket Laupala kohalensis. We compare these two cricket genomes with those of 14 additional insects and find evidence that hemimetabolous genomes expanded due to transposable element activity. Based on the ratio of observed to expected CpG sites, we find higher conservation and stronger purifying selection of methylated genes than non-methylated genes. Finally, our analysis suggests an expansion of the pickpocket class V gene family in crickets, which we speculate might play a role in the evolution of cricket courtship, including their characteristic chirping. Communications biology 4 1 733 733 20210600 10.1038/s42003-021-02197-9 34127782 ishimaruyoshiyasu/published_papers/22950653 Ishimaru Y Tomonari S Watanabe T Noji S Mito T Regulatory mechanisms underlying the specification of the pupal-homologous stage in a hemimetabolous insect. Juvenile hormones and the genetic interaction between the transcription factors Krüppel homologue 1 (Kr-h1) and Broad (Br) regulate the transformation of insects from immature to adult forms in both types of metamorphosis (holometaboly with a pupal stage versus hemimetaboly with no pupal stage); however, knowledge about the exact instar in which this occurs is limited. Using the hemimetabolous cricket Gryllus bimaculatus (Gb), we demonstrate that a genetic interaction occurs among Gb'Kr-h1, Gb'Br and the adult-specifier transcription factor Gb'E93 from the sixth to final (eighth) nymphal instar. Gb'Kr-h1 and Gb'Br mRNAs were strongly expressed in the abdominal tissues of sixth instar nymphs, with precocious adult moults being induced by Gb'Kr-h1 or Gb'Br knockdown in the sixth instar. The depletion of Gb'Kr-h1 or Gb'Br upregulates Gb'E93 in the sixth instar. By contrast, Gb'E93 knockdown at the sixth instar prevents nymphs transitioning to adults, instead producing supernumerary nymphs. Gb'E93 also represses Gb'Kr-h1 and Gb'Br expression in the penultimate nymphal instar, demonstrating its important role in adult differentiation. Our results suggest that the regulatory mechanisms underlying the pupal transition in holometabolous insects are evolutionarily conserved in hemimetabolous G. bimaculatus, with the penultimate and final nymphal periods being equivalent to the pupal stage. This article is part of the theme issue 'The evolution of complete metamorphosis'. Philosophical transactions of the Royal Society of London. Series B, Biological sciences 0962-8436 374 1783 20190225 20190225 20191000 10.1098/rstb.2019.0225 31438810 ishimaruyoshiyasu/published_papers/21711172 Ishimaru Y Bando T Ohuchi H Noji S Mito T Bone morphogenetic protein signaling in distal patterning and intercalation during leg regeneration of the cricket, Gryllus bimaculatus. Development, growth & differentiation 0012-1592 60 6 377 386 20180800 10.1111/dgd.12560 30043459 ishimaruyoshiyasu/published_papers/21711173 Tetsuya Bando Taro Mito Yoshimasa Hamada Yoshiyasu Ishimaru Sumihare Noji Hideyo Ohuchi Molecular mechanisms of limb regeneration: insights from regenerating legs of the cricket Gryllus bimaculatus. This review summarizes recent advances in leg regeneration research, focusing on the cricket Gryllus bimaculatus. Recent studies have revealed molecular mechanisms on blastema formation, establishment of positional information, and epigenetic regulation during leg regeneration. Especially, these studies have provided molecular bases in classical conceptual models such as the polar coordinate model, the intercalation model, the boundary model, the steepness model, etc., which were proposed to interpret regeneration processes of the cockroach legs. When a leg is amputated, a blastema is formed through the activation of the Janus-kinase (Jak)/Signal-Transduction-and-Activator-of-Transcription (STAT) pathway. Subsequently, the Hedgehog/Wingless/Decapentaplegic/Epidermal-growth-factor pathways instruct distalization in the blastema, designated as the molecular boundary model. Downstream targets of this pathway are transcription factors Distal-less (Dll) and dachshund (dac), functioning as key regulators of proximodistal pattern formation. Dll and dac specify the distal and proximal regions in the blastema, respectively, through the regulation of tarsal patterning genes. The expression of leg patterning genes during regeneration may be epigenetically controlled by histone H3K27 methylation via Enhancer-of-zeste and Ubiquitously-transcribed-tetratricopeptide-repeat-gene-X-chromosome. For the molecular mechanism of intercalation of the missing structures between the amputated position and the most distal one, Dachsous/Fat (Ds/Ft) steepness model has been proposed, in which the Ds/Ft pathway maintains positional information and determines leg size through dac expression. This model was theoretically verified to interpret the experimental results obtained with cricket legs. Availability of whole-genome sequence information, regeneration-dependent RNA interference, and genome editing technique will have the cricket be an ideal model system to reveal gene functions in leg regeneration. The International journal of developmental biology 0214-6282 62 6-7-8 559 569 20180000 10.1387/ijdb.180048ho 29938767 ishimaruyoshiyasu/published_papers/21711174 Hiroko Awata Ryo Wakuda Yoshiyasu Ishimaru Yuji Matsuoka Kanta Terao Satomi Katata Yukihisa Matsumoto Yoshitaka Hamanaka Sumihare Noji Taro Mito Makoto Mizunami Roles of OA1 octopamine receptor and Dop1 dopamine receptor in mediating appetitive and aversive reinforcement revealed by RNAi studies SCIENTIFIC REPORTS 2045-2322 6 29696 null 20160700 10.1038/srep29696 27412401 ishimaruyoshiyasu/published_papers/21711175 Yoshiyasu Ishimaru Ishimaru Y Sayuri Tomonari Tomonari S Yuji Matsuoka Matsuoka Y Takahito Watanabe Watanabe T Katsuyuki Miyawaki Miyawaki K Tetsuya Bando Bando T Kenji Tomioka Tomioka K Hideyo Ohuchi Ohuchi H Sumihare Noji Noji S Taro Mito Mito T TGF-beta signaling in insects regulates metamorphosis via juvenile hormone biosynthesis TGF-β signaling in insects regulates metamorphosis via juvenile hormone biosynthesis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Proceedings of the National Academy of Sciences of the United States of America 0027-8424 113 20 5634 5639 20160500 10.1073/pnas.1600612113 27140602 ishimaruyoshiyasu/published_papers/21711176 Yoshimasa Hamada Tetsuya Bando Taro Nakamura Yoshiyasu Ishimaru Taro Mito Sumihare Noji Kenji Tomioka Hideyo Ohuchi Leg regeneration is epigenetically regulated by histone H3K27 methylation in the cricket Gryllus bimaculatus DEVELOPMENT 1477-9129 142 17 2916 + 20150900 10.1242/dev.122598 26253405 ishimaruyoshiyasu/published_papers/21711177 Yuji Matsuoka Tetsuya Bando Takahito Watanabe Yoshiyasu Ishimaru Sumihare Noji Aleksandar Popadic Taro Mito Short germ insects utilize both the ancestral and derived mode of Polycomb group-mediated epigenetic silencing of Hox genes BIOLOGY OPEN 2046-6390 4 6 702 709 20150600 10.1242/bio.201411064 25948756 ishimaruyoshiyasu/published_papers/21711178 Yoshiyasu Ishimaru Taro Nakamura Tetsuya Bando Yuji Matsuoka Hideyo Ohuchi Sumihare Noji Taro Mito Involvement of dachshund and Distal-less in distal pattern formation of the cricket leg during regeneration SCIENTIFIC REPORTS 2045-2322 5 8387 null 20150200 10.1038/srep08387 25669615 ishimaruyoshiyasu/published_papers/21711179 Tetsuya Bando Yoshiyasu Ishimaru Takuro Kida Yoshimasa Hamada Yuji Matsuoka Taro Nakamura Hideyo Ohuchi Sumihare Noji Taro Mito Analysis of RNA-Seq data reveals involvement of JAK/STAT signalling during leg regeneration in the cricket Gryllus bimaculatus DEVELOPMENT 0950-1991 140 5 959 964 20130300 10.1242/dev.084590 23344706 ishimaruyoshiyasu/published_papers/21711180 Yoshiyasu Ishimaru Tomoko Komatsu Megumi Kasahara Yuko Katoh-Fukui Hidesato Ogawa Yoshiro Toyama Mamiko Maekawa Kiyotaka Toshimori Roshantha A. S. Chandraratna Ken-Ichirou Morohashi Hidefumi Yoshioka Mechanism of asymmetric ovarian development in chick embryos DEVELOPMENT 1477-9129 135 4 677 685 20080200 10.1242/dev.012856 18199582 ishimaruyoshiyasu/published_papers/21711181 H Yoshioka Y Ishimaru N Sugiyama N Tsunekawa T Noce M Kasahara K Morohashi Mesonephric FGF signaling is associated with the development of sexually indifferent gonadal primordium in chick embryos DEVELOPMENTAL BIOLOGY 0012-1606 280 1 150 161 20050400 10.1016/j.ydbio.2005.01.011 15766755 ishimaruyoshiyasu/published_papers/25758631 Yoshioka H Ishimaru Y Sugiyama N Kasahara M Morohashi K Mesonephric Wnt signaling associate with a formation of an adreno-gonadal primordium in chick embryos. Endocr Res. 30 523 null 20040000 ishimaruyoshiyasu/published_papers/25758630 Morohashi K Ishimaru Y Sugiyama N Yoshioka H Growth factors from mesonephros implicated in gonadal and adrenal differentiation. Endocr Res. 30 511 null 20040000 ishimaruyoshiyasu/published_papers/21757907 Y Ishimaru H Yoshioka H Tao B Thisse C Thisse CVE Wright H Hamada H Ohuchi S Noji Asymmetric expression of antivin/lefty1 in the early chick embryo MECHANISMS OF DEVELOPMENT 0925-4773 90 1 115 118 20000100 ishimaruyoshiyasu/published_papers/21757925 H Yoshioka C Meno K Koshiba M Sugihara H Itoh Y Ishimaru T Inoue H Ohuchi EV Semina JC Murray H Hamada S Noji Pitx2, a bicoid-type homeobox gene, is involved in a lefty-signaling pathway in determination of left-right asymmetry CELL 0092-8674 94 3 299 305 19980800 ishimaruyoshiyasu/published_papers/21757929 H Ohuchi J Takeuchi H Yoshioka Y Ishimaru K Ogura N Takahashi T Ogura S Noji Correlation of wing-leg identity in ectopic FGF-induced chimeric limbs with the differential expression of chick Tbx5 and Tbx4 DEVELOPMENT 0950-1991 125 1 51 60 19980100 ishimaruyoshiyasu/published_papers/21757976 H Ohuchi T Nakagawa A Yamamoto A Araga T Ohata Y Ishimaru H Yoshioka T Kuwana T Nohno M Yamasaki N Itoh S Noji The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor DEVELOPMENT 0950-1991 124 11 2235 2244 19970600 ishimaruyoshiyasu/published_papers/21757979 H Yoshioka H Ohuchi Y Ishimaru T Ishikawa T Nohno K Saigo S Noji A Drosophila receptor-type tyrosine kinase (DFR1) acts as a fibroblast growth factor receptor in Xenopus embryos DEVELOPMENT GROWTH & DIFFERENTIATION 0012-1592 38 6 617 624 19961200 ishimaruyoshiyasu/misc/52454962 井上智晶 石丸善康 川本晃平 井上慎太郎 渡邉崇人 野地澄晴 三戸太郎 フタホシコオロギをモデルとした不完全変態昆虫におけるE93遺伝子の発現と成虫化に関わる機能の解析 日本分子生物学会年会プログラム・要旨集(Web) 47th null null 20240000 ishimaruyoshiyasu/misc/52454958 島村彩音 井上慎太郎 石丸善康 宮脇克行 高橋章 二川健 野地澄晴 渡邉崇人 三戸太郎 フタホシコオロギの体色パターン形成における白色スクレロチン合成酵素遺伝子の発現と機能の解析 日本分子生物学会年会プログラム・要旨集(Web) 47th null null 20240000 ishimaruyoshiyasu/misc/897904 Ishimaru Yoshiyasu 石丸 善康 Noji Sumihare 野地 澄晴 Miti Taro 三戸 太郎 Molecular mechanisms of insect metamorphosis: insights from studies of the cricket Gryllus bimaculatus 昆虫変態の分子機構:コオロギの研究から 昆虫と自然 54 11 38 41 20191000 ishimaruyoshiyasu/misc/28874829 Hidefumi Yoshioka Yoshiyasu Ishimaru Tomoko Komatsu Megumi Kasahara Yuko Katoh-Fukui Ken-ichiro Morohashi Mechanism for asymmetric gonad development in birds ZOOLOGICAL SCIENCE 0289-0003 23 12 1132 1133 20061200 ishimaruyoshiyasu/misc/41593081 H Yoshioka Y Ishimaru N Sugiyama M Kasahara K Morohashi Mesonephric wnt signaling associate with a formation of an adreno-gonadal primordium in chick embryos ENDOCRINE RESEARCH 0743-5800 30 4 523 523 20040000 10.1081/ERC-200043613 15666784 2-s2.0-11244283907 ishimaruyoshiyasu/misc/38987744 ISHIMARU Yoshiyasu 石丸 善康 SUGIHARA Minoru 椙原 穂 YOSHIOKA Hidefumi 吉岡 秀文 ITOH Hiroyuki 伊藤 裕之 OHUCHI Hideyo 田尾 嘉誉 MENO Chikara 大内 淑代 HAMADA Hiroshi 目野 主税 NOJI Sumiharu 濱田 博司 野地 澄晴 A mechanism involved in determination of left-right axis in chick embryos ニワトリ胚の左右軸決定のメカニズム 日本分子生物学会年会プログラム・講演要旨集 21 603 603 19981201 ishimaruyoshiyasu/books_etc/36965355 野地, 澄晴 最先端コオロギ学 : 世界初!新しい生物学がここにある 北隆館 20220400 ishimaruyoshiyasu/books_etc/13678073 石丸 善康 伊藤 伸一 宇野 剛史 岡村 康弘 大平 健司 小野 公輔 掛井 秀一 金西 計英 澤田 麻衣子 蓮沼 徹 古屋 S. 玲 松浦 健二 松本 和幸 土岐 順子 阿部 香織 山崎 紅 辰巳 丈夫 岩元 直久 勝村 幸博 情報科学入門 2019年版 日経BP社, 日経BPマーケティング (発売) 20190000 ishimaruyoshiyasu/books_etc/13678077 石丸 善康 伊藤 伸一 宇野 剛史 岡村 康弘 大平 健司 小野 公輔 掛井 秀一 金西 計英 澤田 麻衣子 蓮沼 徹 古屋 S. 玲 松浦 健二 松本 和幸 土岐 順子 阿部 香織 山崎 紅 辰巳 丈夫 岩元 直久 勝村 幸博 情報科学入門 2018年版 日経BP社, 日経BPマーケティング (発売) 20180000 ishimaruyoshiyasu/books_etc/13678079 石丸 善康 伊藤 伸一 宇野 剛史 岡村 康弘 大平 健司 小野 公輔 掛井 秀一 金西 計英 澤田 麻衣子 蓮沼 徹 古屋 S. 玲 松浦 健二 松本 和幸 土岐 順子 阿部 香織 山崎 紅 辰巳 丈夫 岩元 直久 勝村 幸博 情報科学入門 2017年版 日経BP社, 日経BPマーケティング (発売) 20170000