miho.inoue/published_papers/52817814 西口 翔太 鴨居 浩平 鈴木 善貴 谷脇 竜弥 新開 瑞希 井上 美穂 大倉 一夫 松香 芳三 PETGシートと常温重合レジンを用いた易調整性のハイブリッド型オクルーザルアプライアンスの製作法 テクニカルレポート (NPO)日本睡眠歯科学会 睡眠口腔医学 2433-8524 12 95 95 20250900 miho.inoue/published_papers/52817801 笠井 玲緒 鈴木 善貴 奥野 健太郎 大倉 一夫 小池 一幸 井上 美穂 新開 瑞希 柴垣 あかり 谷脇 竜弥 渡邊 亮友 高橋 一也 松香 芳三 徳島大学・大阪歯科大学歯学科生における睡眠歯科に対する関心の調査 (公社)日本補綴歯科学会 日本補綴歯科学会誌 1883-6860 17 332 332 20250500 miho.inoue/published_papers/52799773 笠井 玲緒 鈴木 善貴 奥野 健太郎 大倉 一夫 小池 一幸 井上 美穂 新開 瑞希 柴垣 あかり 谷脇 竜弥 渡邊 亮友 高橋 一也 松香 芳三 徳島大学・大阪歯科大学歯学科生における睡眠歯科に対する関心の調査 (公社)日本補綴歯科学会 日本補綴歯科学会誌 1883-6860 17 332 332 20250500 miho.inoue/published_papers/50877665 Maki Hosoki Mayu Miyagi Kazuo Okura Miho Inoue Masamitsu Oshima Yoshitaka Suzuki Aya Ozawa Akari Shibagaki Tatsuya Taniwaki Kohei Kamoi Nobuyuki Bando Yasutomo Yoshihara Mizuki Shinkai Toyoko Tajima Jaime Jr Fabillar Yoshizo Matsuka Proposals and evaluations for the enhancement of CAD/CAM education. Journal of prosthodontic research 69 2 133 135 20250414 10.2186/jpr.JPR_D_24_00132 39443134 miho.inoue/published_papers/50877664 Toyoko Tajima Maki Hosoki Mayu Miyagi Miho Inoue Aya Ozawa Mizuki Shinkai Mio Naritani Yoshiaki Kubo Swarnalakshmi Raman Parimal Ravindra Chavan Kazuyuki Koike Yoshizo Matsuka Correlation between pierced earrings and the prevalence of metal allergies at Tokushima university hospital: a 15-year retrospective analysis. In Japan, metal allergies are becoming increasingly prevalent, raising concerns for public health. This study examined metal allergy characteristics, patient histories, and clinical signs associated with patch test results over a 15-year period at the Dental Metal Allergy Clinic of Tokushima University Hospital. A retrospective analysis of 1085 patients revealed that 65.4% tested positive for at least one metal allergen, with palladium chloride, nickel sulfate, potassium dichromate, and cobalt chloride identified as the most common allergens. Female patients were disproportionately affected, accounting for 78.4% of the study population. Notably, there was a substantial increase in patients reporting inflammation due to pierced earrings, increasing from 5.0% in 2005 to 43.2% in 2020, particularly among females. Patients with a history of inflammation from earrings had an 81.3% prevalence of metal allergies, which was significantly higher than the 60.4% reported in those without such a history (chi-square test, p < 0.001). These findings suggest a strong link between earrings and metal allergies, underscoring the need for improved education, early detection, and preventive strategies to address the growing impact of metal allergies on public health. Scientific reports 15 1 10939 10939 20250329 10.1038/s41598-025-86868-1 40157960 miho.inoue/published_papers/52817832 谷脇 竜弥 細木 真紀 宮城 麻友 板東 伸幸 大島 正充 鈴木 善貴 井上 美穂 生田目 大介 小澤 彩 青木 映璃 吉原 靖智 大倉 一夫 新開 瑞希 柴垣 あかり 松香 芳三 クラウンブリッジ補綴学模型実習の改善 アンケート調査に基づく分析 (公社)日本補綴歯科学会 日本補綴歯科学会誌 1883-6860 16 333 333 20240700 miho.inoue/published_papers/52707230 小澤 彩 鈴木 善貴 大倉 一夫 田島 登誉子 谷脇 竜弥 新開 瑞希 柴垣 あかり 井上 美穂 大川 敏永 安陪 晋 重本 修伺 松香 芳三 睡眠時ブラキシズムはオクルーザルアプライアンスを変形させるのか (公社)日本補綴歯科学会 日本補綴歯科学会誌 1883-6860 16 295 295 20240700 miho.inoue/published_papers/52817869 細木 真紀 生田目 大介 宮城 麻友 大倉 一夫 井上 美穂 小澤 彩 柴垣 あかり 谷脇 竜弥 鴨居 浩平 板東 伸幸 吉原 靖智 大島 正充 鈴木 善貴 新開 瑞希 松香 芳三 デジタル化に対応したクラウンブリッジ補綴学実習の検討 (公社)日本補綴歯科学会 日本補綴歯科学会誌 1883-6860 15 294 294 20230500 miho.inoue/published_papers/52817862 小澤 彩 鈴木 善貴 田島 登誉子 大川 敏永 鴨居 浩平 大倉 一夫 谷脇 竜弥 井上 美穂 吉原 靖智 大島 正充 安陪 晋 松香 芳三 睡眠時ブラキシズム患者に装着したアプライアンスの変形を観察した症例 (公社)日本補綴歯科学会 日本補綴歯科学会誌 1883-6860 15 307 307 20230500 miho.inoue/published_papers/52817908 大倉 一夫 葉山 莉香 大島 正充 細木 眞紀 鈴木 善貴 宮城 麻友 井上 美穂 生田目 大介 田島 登誉子 吉原 靖智 小澤 彩 新開 瑞希 松香 芳三 歯科補綴学におけるアクティブラーニングの学修効果 反転授業とTBL，通常授業の比較 (公社)日本補綴歯科学会 日本補綴歯科学会誌 1883-6860 14 311 311 20220700 miho.inoue/published_papers/52817901 新開 瑞希 細木 真紀 鈴木 善貴 田島 登誉子 井上 美穂 小澤 彩 吉原 靖智 大島 正充 織田 英正 本田 常晴 松香 芳三 レジンアレルギーのリスクと歯科治療における対応 (公社)日本補綴歯科学会 日本補綴歯科学会誌 1883-6860 14 345 345 20220700 miho.inoue/published_papers/52817922 大倉 一夫 井上 美穂 鈴木 善貴 細木 眞紀 安陪 晋 河野 文昭 松香 芳三 歯科補綴学授業におけるアクティブラーニングの導入とその学修効果 オンライン授業とTBL授業の比較 (一社)日本歯科医学教育学会 日本歯科医学教育学会総会・学術大会プログラム・抄録集 40 67 67 20211100 miho.inoue/published_papers/52817917 細木 眞紀 大倉 一夫 井上 美穂 鈴木 善貴 安陪 晋 河野 文昭 松香 芳三 コロナ禍におけるデジタル機器およびウェブを利用した歯科補綴学実習の評価 (一社)日本歯科医学教育学会 日本歯科医学教育学会総会・学術大会プログラム・抄録集 40 75 75 20211100 miho.inoue/published_papers/50877666 Huijiao Yan Masamitsu Oshima Resmi Raju Swarnalakshmi Raman Kazumitsu Sekine Arief Waskitho Miho Inoue Masahisa Inoue Otto Baba Tsuyoshi Morita Mayu Miyagi Yoshizo Matsuka Dentin-Pulp Complex Tissue Regeneration via Three-Dimensional Cell Sheet Layering. The dentin-pulp complex is a unique structure in teeth that contains both hard and soft tissues. Generally, deep caries and trauma cause damage to the dentin-pulp complex, and if left untreated, this damage will progress to irreversible pulpitis. The aim of this study was to fabricate a layered cell sheet composed of rat dental pulp (DP) cells and odontogenic differentiation of pulp (OD) cells and to investigate the ability to regenerate the dentin-pulp complex in a scaffold tooth. We fabricated two single cell sheets composed of DP cells (DP cell sheet) or OD cells (OD cell sheet) and a layered cell sheet made by layering both cells. The characteristics of the fabricated cell sheets were analyzed using light microscopy, scanning electron microscope (SEM), hematoxylin-eosin (HE) staining, and immunohistochemistry (IHC). Furthermore, the cell sheets were transplanted into the subrenal capsule of immunocompromised mice for 8 weeks. After this, the regenerative capacity to form dentin-like tissue was evaluated using micro-computed tomography (micro-CT), HE staining, and IHC. The findings of SEM and IHC confirmed that layered cell sheets fabricated by stacking OD cells and DP cells maintained their cytological characteristics. Micro-CT of layered cell sheet transplants revealed a mineralized capping of the access cavity in the crown area, similar to that of natural dentin. In contrast, the OD cell sheet group demonstrated the formation of irregular fragments of mineralized tissue in the pulp cavity, and the DP cell sheet did not develop any hard tissue. Moreover, bone volume/tissue volume (BV/TV) showed a significant increase in hard tissue formation in the layered cell sheet group compared with that in the single cell sheet group (p < 0.05). HE staining also showed a combination of soft and hard tissue formation in the layered cell sheet group. Furthermore, IHC confirmed that the dentin-like tissue generated from the layered cell sheet expressed characteristic markers of dentin but not bone equivalent to that of a natural tooth. In conclusion, this study demonstrates the feasibility of regenerating dentin-pulp complex using a bioengineered tissue designed to simulate the anatomical structure. Impact statement The dentin-pulp complex can be destroyed by deep caries and trauma, which may cause pulpitis and progress to irreversible pulpitis, apical periodontitis, and even tooth loss. Current treatments cannot maintain pulp health, and teeth can become brittle. We developed a three-dimensional (3D) layered cell sheet using dental pulp cells and odontogenic differentiation of pulp cells for dentin-pulp complex regeneration. Our layered cell sheet enables the regeneration of an organized 3D dentin-pulp-like structure comparable with that of natural teeth. This layered cell sheet technology may contribute to dentin-pulp complex regeneration and provide a novel method for complex tissue engineering. Tissue engineering. Part C, Methods 27 10 559 570 20211000 10.1089/ten.TEC.2021.0171 34583551 miho.inoue/published_papers/50877667 Hiroo Kawahara Miho Inoue Kazuo Okura Masamitsu Oshima Yoshizo Matsuka Risk Factors for Tooth Loss in Patients with ≥25 Remaining Teeth Undergoing Mid-Long-Term Maintenance: A Retrospective Study. Tooth loss represents a diffused pathologic condition affecting the worldwide population. Risk factors have been identified in both general features (smoking, diabetes, economic status) and local tooth-related factors (caries, periodontitis). In this retrospective study, we examined the data of 366 patients with a large number of remaining teeth (≥25) undergoing maintenance therapy in order to identify specific risk factors for tooth loss. The number of remaining teeth, number of non-vital teeth, and number of occlusal units were investigated for their correlation with tooth loss. The mean follow-up of patients was 9.2 years (range 5 to 14). Statistically significant risk factors for tooth loss were identified as number of remaining teeth at baseline (p = 0.05), number of occlusal units (p = 0.03), and number of non-vital teeth in posterior regions (p < 0.001). Multiple logistic regression showed that the number of occlusal units and number of non-vital teeth in the posterior regions were significantly associated with a greater risk of tooth loss (odds ratio 1.88 and 3.17, respectively). These results confirm that not only the number of remaining teeth, but also their vital or non-vital status and the distribution between the anterior and posterior regions influence the long-term survival. International journal of environmental research and public health 18 13 null null 20210705 10.3390/ijerph18137174 34281111 miho.inoue/published_papers/52817933 吉原 靖智 鈴木 善貴 大倉 一夫 重本 修司 安陪 晋 大島 正充 細木 真紀 井上 美穂 坂東 永一 松香 芳三 顎運動を伴うRMMAの三次元顎運動解析を行った症例 (公社)日本補綴歯科学会 日本補綴歯科学会誌 1883-6860 13 185 185 20210600 miho.inoue/published_papers/50877668 Hiroo Kawahara Miho Inoue Kazuo Okura Masamitsu Oshima Yoshizo Matsuka Risk Factors for Tooth Loss in Patients Undergoing Mid-Long-Term Maintenance: A Retrospective Study. In this retrospective study, we identified risk factors for tooth loss in patients undergoing mid-long-term maintenance therapy. We surveyed 674 maintenance patients for ≥5 years after active treatment who visited a dental clinic between January 2015 and December 2016. Of these, 265 were men (mean age 54.6 ± 8.0 years old) and 409 were women (mean age 54.0 ± 7.9 years old). Study variables included patient compliance, sex, number of teeth lost, cause of tooth loss (dental caries, periodontal disease, root fracture, others, vital or non-vital teeth), age at start of maintenance, number of remaining teeth at start of maintenance, smoking, use of salivary secretion inhibitors, presence of diabetes mellitus, condition of periodontal bone loss, and use of a removable denture. Most lost teeth were non-vital teeth (91.7% of all cases) and the most common cause of tooth loss was tooth fracture (62.1% of all cases). A statistically significant risk factors for tooth loss was number of remaining teeth at the start of maintenance (p = 0.003). International journal of environmental research and public health 17 17 null null 20200827 10.3390/ijerph17176258 32867387 miho.inoue/published_papers/50877669 Resmi Raju Masamitsu Oshima Miho Inoue Tsuyoshi Morita Yan Huijiao Arief Waskitho Otto Baba Masahisa Inoue Yoshizo Matsuka Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet. Periodontal tissue is a distinctive tissue structure composed three-dimensionally of cementum, periodontal ligament (PDL) and alveolar bone. Severe periodontal diseases cause fundamental problems for oral function and general health, and conventional dental treatments are insufficient for healing to healthy periodontal tissue. Cell sheet technology has been used in many tissue regenerations, including periodontal tissue, to transplant appropriate stem/progenitor cells for tissue regeneration of a target site as a uniform tissue. However, it is still difficult to construct a three-dimensional structure of complex tissue composed of multiple types of cells, and the transplantation of a single cell sheet cannot sufficiently regenerate a large-scale tissue injury. Here, we fabricated a three-dimensional complex cell sheet composed of a bone-ligament structure by layering PDL cells and osteoblast-like cells on a temperature responsive culture dish. Following ectopic and orthotopic transplantation, only the complex cell sheet group was demonstrated to anatomically regenerate the bone-ligament structure along with the functional connection of PDL-like fibers to the tooth root and alveolar bone. This study represents successful three-dimensional tissue regeneration of a large-scale tissue injury using a bioengineered tissue designed to simulate the anatomical structure. Scientific reports 10 1 1656 1656 20200203 10.1038/s41598-020-58222-0 32015383 miho.inoue/published_papers/50877670 Takuma Iwasa Shaista Afroz Miho Inoue Rieko Arakaki Masamitsu Oshima Resmi Raju Arief Waskitho Masahisa Inoue Otto Baba Yoshizo Matsuka IL-10 and CXCL2 in trigeminal ganglia in neuropathic pain. Many trigeminal neuropathic pain patients suffer severe chronic pain. The neuropathic pain might be related with cross-excitation of the neighboring neurons and satellite glial cells (SGCs) in the sensory ganglia and increasing the pain signals from the peripheral tissue to the central nervous system. We induced trigeminal neuropathic pain by infraorbital nerve constriction injury (IONC) in Sprague-Dawley rats. We tested cytokine (CXCL2 and IL-10) levels in trigeminal ganglia (TGs) after trigeminal neuropathic pain induction, and the effect of direct injection of the anti-CXCL2 and recombinant IL-10 into TG. We found that IONC induced pain behavior. Additionally, IONC induced satellite glial cell activation in TG and cytokine levels of TGs were changed after IONC. CXCL2 levels increased on day 1 of neuropathic pain induction and decreased gradually, with IL-10 levels showing the opposite trend. Recombinant IL-10 or anti-CXCL2 injection into TG decreased pain behavior. Our results show that IL-10 or anti-CXCL2 are therapy options for neuropathic pain. Neuroscience letters 703 132 138 20190611 10.1016/j.neulet.2019.03.031 30904573 miho.inoue/published_papers/50877671 Shaista Afroz Rieko Arakaki Takuma Iwasa Masamitsu Oshima Maki Hosoki Miho Inoue Otto Baba Yoshihiro Okayama Yoshizo Matsuka CGRP Induces Differential Regulation of Cytokines from Satellite Glial Cells in Trigeminal Ganglia and Orofacial Nociception. Neuron-glia interactions contribute to pain initiation and sustainment. Intra-ganglionic (IG) secretion of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) modulates pain transmission through neuron-glia signaling, contributing to various orofacial pain conditions. The present study aimed to investigate the role of satellite glial cells (SGC) in TG in causing cytokine-related orofacial nociception in response to IG administration of CGRP. For that purpose, CGRP alone (10 μL of 10-5 M), Minocycline (5 μL containing 10 μg) followed by CGRP with one hour gap (Min + CGRP) were administered directly inside the TG in independent experiments. Rats were evaluated for thermal hyperalgesia at 6 and 24 h post-injection using an operant orofacial pain assessment device (OPAD) at three temperatures (37, 45 and 10 °C). Quantitative real-time PCR was performed to evaluate the mRNA expression of IL-1β, IL-6, TNF-α, IL-1 receptor antagonist (IL-1RA), sodium channel 1.7 (NaV 1.7, for assessment of neuronal activation) and glial fibrillary acidic protein (GFAP, a marker of glial activation). The cytokines released in culture media from purified glial cells were evaluated using antibody cytokine array. IG CGRP caused heat hyperalgesia between 6⁻24 h (paired-t test, p < 0.05). Between 1 to 6 h the mRNA and protein expressions of GFAP was increased in parallel with an increase in the mRNA expression of pro-inflammatory cytokines IL-1β and anti-inflammatory cytokine IL-1RA and NaV1.7 (one-way ANOVA followed by Dunnett's post hoc test, p < 0.05). To investigate whether glial inhibition is useful to prevent nociception symptoms, Minocycline (glial inhibitor) was administered IG 1 h before CGRP injection. Minocycline reversed CGRP-induced thermal nociception, glial activity, and down-regulated IL-1β and IL-6 cytokines significantly at 6 h (t-test, p < 0.05). Purified glial cells in culture showed an increase in release of 20 cytokines after stimulation with CGRP. Our findings demonstrate that SGCs in the sensory ganglia contribute to the occurrence of pain via cytokine expression and that glial inhibition can effectively control the development of nociception. International journal of molecular sciences 20 3 null null 20190207 10.3390/ijms20030711 30736422 miho.inoue/published_papers/50877672 Keisuke Nishigawa Rika Hayama Katsuhiro Omoto Kazuo Okura Toyoko Tajima Yoshitaka Suzuki Maki Hosoki Mayu Ueda Miho Inoue Omar Marianito Maningo Rodis Yoshizo Matsuka Validity of Peer Evaluation for Team-Based Learning in a Dental School in Japan. The aim of this study was to determine the validity of peer evaluation for team-based learning (TBL) classes in dental education in comparison with the term-end examination records and TBL class scores. Examination and TBL class records of 256 third- and fourth-year dental students in six fixed prosthodontics courses from 2013 to 2015 in one dental school in Japan were investigated. Results of the term-end examination during those courses, individual readiness assurance test (IRAT), group readiness assurance test (GRAT), group assignment projects (GAP), and peer evaluation of group members in TBL classes were collected. Significant positive correlations were found between all combinations of peer evaluation, IRAT, and term-end examination. Individual scores also showed a positive correlation with group score (total of GRAT and GAP). From the investigation of the correlations in the six courses, significant positive correlations between peer evaluation and individual score were found in four of the six courses. In this study, peer evaluation seemed to be a valid index for learning performance in TBL classes. To verify the effectiveness of peer evaluation, all students have to realize the significance of scoring the team member's performance. Clear criteria and detailed instruction for appropriate evaluation are also required. Journal of dental education 81 12 1451 1456 20171200 10.21815/JDE.017.106 29196333 miho.inoue/published_papers/50877673 Shintaro Sukegawa Hitoshi Higuchi Miho Inoue Hitoshi Nagatsuka Shigeru Maeda Takuya Miyawaki Locally injected dexmedetomidine inhibits carrageenin-induced inflammatory responses in the injected region. BACKGROUND: Dexmedetomidine, a highly selective agonist of α2-adrenoceptors, is a commonly used sedative; however, a potent anti-inflammatory effect has also been found. In the present study we evaluated the inhibitory effect of locally injected dexmedetomidine on inflammatory responses in the injected region. METHODS: Local inflammation was induced in the hindpaws of male mice (aged 6-8 weeks) by intraplantar injection of lambda-carrageenin. To offset the central effect of tested agents, different agents were blindly injected into the left and right paws in the pairs of comparison. The effect of dexmedetomidine on edema (increase in paw volume), the accumulation of leukocytes, and production of tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2) were evaluated after carrageenin injection, using water displacement plethysmometry, histological imaging, immunohistochemistry, and Western blotting analysis. Furthermore, we also evaluated the effect of yohimbine, a full antagonist of α2-adrenoceptors, and phenylephrine, an agonist of the α1-adrenoceptor, on dexmedetomidine's action on inflammatory responses. RESULTS: Paw volume and amount of leukocytes in the injected region significantly increased after the injection of carrageenin. Similarly, TNF-α and COX-2 production was found in the subcutaneous region injected with carrageenin, 4 hours after injection. Dexmedetomidine significantly inhibited all increases in paw volume, leukocytes, and production of TNF-α and COX-2. Furthermore, yohimbine significantly antagonized the anti-inflammatory effects of dexmedetomidine, whereas phenylephrine did not significantly alter them. CONCLUSIONS: The findings suggest that locally injected dexmedetomidine exhibits an anti-inflammatory effect against local acute inflammatory responses, mediated by α2-adrenoceptors. Anesthesia and analgesia 118 2 473 480 20140200 10.1213/ANE.0000000000000060 24445644 miho.inoue/published_papers/51032171 Miho Inoue Andrea P Rodriguez Noriyuki Nagai Hitoshi Nagatsuka Racquel Z LeGeros Hidetsugu Tsujigiwa Masahisa Inoue Etsuo Kishimoto Shin Takagi Effect of fluoride-substituted apatite on in vivo bone formation. Biological apatites are characterized by the presence of minor constituents such as magnesium (Mg), chloride (Cl), or fluoride (F) ions. These ions affect cell proliferation and osteoblastic differentiation during bone tissue formation. F-substituted apatites are being explored as potential bonegraft materials. The aim of the present study is to investigate the mechanism of bone formation induced by fluoride-substituted apatite (FAp) by analyzing the effect of FAp on the process of in vivo bone formation. FAps containing different F concentrations (l-FAp: 0.48 wt%, m-FAp: 0.91 wt%, h-FAp: 2.23 wt%) and calcium-deficient apatite (CDA), as positive control, were implanted in rat tibia and bone formation was evaluated by histological examination, immuhistochemistry, in situ hybridization and tartrate-resistant acid phosphatase examinations. The results showed that l-FAp, m-FAp, h-FAp, and CDA biomaterials allowed migration of macrophages, attachment, proliferation, and phenotypic expression of bone cells leading to new bone formation in direct apposition to the particles. However, the l-FAp preparation allowed faster bone conduction compared to the other experimental materials. These results suggest that FAp with low F concentration may be an efficient bonegraft material for dental and medical application. Journal of biomaterials applications 25 8 811 24 20110500 10.1177/0885328209357109 20219846 miho.inoue/published_papers/51032173 Andrea P Rodriguez Miho Inoue Toshiyuki Tanaka Michihiro Miyake Ana M Sfer Etsuo Kishimoto Hidetsugu Tsujigiwa Rosario S Rivera Hitoshi Nagatsuka Effect of CaTiO(3)-CaCO(3) prepared by alkoxide method on cell response. In recent years, calcium titanate (CaTiO(3)) and carbon-containing materials have gained much attention in a number of biomedical material researches. To maximize the advantages of both materials, we developed a novel alkoxide method to get "calcium titanate with calcium carbonate" (CaTiO(3)-CaCO(3)). The objective was to evaluate the crystallinity and elemental composition of CaTiO(3)-CaCO(3) prepared by alkoxide method, CaTiO(3)-aC elaborated by modified thermal decomposition method, commercially-prepared CaTiO(3), and the effect of these materials on the bone marrow stromal cell. Hydroxyapatite was used as positive control material. We examined the cellular proliferation, osteoblastic differentiation, and mineralization of KUSA/A1 cells cultured with the materials. The results showed that CaTiO(3)-CaCO(3) and CaTiO(3)-aC contained evidence of calcium carbonate enhancing cell proliferation, osteoblastic differentiation, and mineralization. On the contrary, the commercially-prepared CaTiO(3) revealed absence of calcium carbonate with lower cell response than the other groups. The results indicated that calcium carbonate could play a key role in the cell response of CaTiO(3) material. In conclusion, our findings suggest that CaTiO(3)-CaCO(3) could be considered an important candidate as a biomaterial for medical and dental applications. Journal of biomedical materials research. Part A 93 1 297 303 20100400 10.1002/jbm.a.32551 19562752 miho.inoue/published_papers/51032172 Miho Inoue Andrea P Rodriguez Tohru Takagi Naoki Katase Midori Kubota Noriyuki Nagai Hitoshi Nagatsuka Masahisa Inoue Noriyuki Nagaoka Shin Takagi Kazuomi Suzuki Effect of a new titanium coating material (CaTiO3-aC) prepared by thermal decomposition method on osteoblastic cell response. Titanium and hydroxyapatite (HA) are widely used as biomaterials for dental and medical applications. HA-coated titanium implants have excellent biocompatibility and mechanical properties. However, the adherence of HA film formed on titanium substrate is weak because of the lack of chemical interaction between HA and titanium. A solution to this problem is to form an intermediate film on titanium substrate, which provide excellent adherence to both titanium substrate and HA. We developed a novel biomaterial called calcium titanate-amorphous carbon (CaTiO(3)-aC) coating prepared by modified thermal decomposition method. The purpose of this study was to evaluate the effect of CaTiO(3)-aC and HA coating (positive control), and Ti (negative control) on osteoblastic (MT3T3-E1) cell responses. An increased cellular proliferation was observed in CaTiO(3)-aC coating compared to HA coating. The maximum expressions of ALP activity, Col I and ALP mRNA were higher and achieved in shorter period of time in CaTiO(3)-aC coating compared to others. These results demonstrated that CaTiO(3)-aC promoted better cell attachment, cellular proliferation, and osteoblastic differentiation compared with HA. In conclusion, we suggested that CaTiO(3)-aC could be considered as an important candidate as a coating material. Journal of biomaterials applications 24 7 657 72 20100300 10.1177/0885328209340334 19726530 miho.inoue/published_papers/51032175 Gulsan Ara Sathi Miho Inoue Hidemitsu Harada Andrea P Rodriguez Ryo Tamamura Hidetsugu Tsujigiwa Silvia S Borkosky Mehmet Gunduz Hitoshi Nagatsuka Secreted frizzled related protein (sFRP)-2 inhibits bone formation and promotes cell proliferation in ameloblastoma. Secreted frizzled related protein (sFRP)-2, a Wnt antagonist, was strongly expressed by both stromal and tumor cells of ameloblastoma. The aim of this study is to evaluate whether sFRP-2 secreted from tumor cells have any direct role in suppressed bone formation or not. A pre-osteoblastic cell line, KUSA/A1 cells, cultured in conditioned medium of an ameloblastoma-derived cell line (AM-1CM) was used in the study. Alkaline phosphatase (ALP) activity, alizarin red staining, mineral quantification and MTS assay was performed. Wnt-canonical pathway is a major pathway for osteoblasts. Antagonists of this pathway, sFRP-1, 2 and 3, were detected by immunohistochemistry and western blot analysis. KUSA/A1 cells cultured in AM-1CM showed high cell proliferation, low ALP activity without mineralized matrix deposition. sFRP-2 was strongly expressed in ameloblastoma tissue and AM-1 cells. After sFRP-2 depletion, the cells showed diffuse mineralization. In this study, it was confirmed that ameloblastoma cells have a major role in decreased bone formation by secreting sFRP-2 in cell culture model. Though, sFRP-2 has great effect on tumor progression, inhibition of sFRP-2's anti-bone formation activity and cell proliferative activity may reduce the invasive property of ameloblastoma and possibility of recurrence rate. Oral oncology 45 10 856 60 20091000 10.1016/j.oraloncology.2009.02.001 19362047 miho.inoue/published_papers/51032174 Andrea P Rodriguez Hidetsugu Tsujigiwa Mehmet Gunduz Beyhan Cengiz Noriyuki Nagai Ryo Tamamura Silvia S Borkosky Tohru Takagi Miho Inoue Hitoshi Nagatsuka Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells. Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cells, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo. Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al 0327-9545 33 1 39 47 20090400 19499885 miho.inoue/published_papers/51032176 Chisa Kuroda Satoshi Kubota Kazumi Kawata Eriko Aoyama Kumi Sumiyoshi Morihiko Oka Miho Inoue Shogo Minagi Masaharu Takigawa Distribution, gene expression, and functional role of EphA4 during ossification. EphA4 receptor tyrosine kinase has been shown to be critically involved in neural tissue development. Here, we found EphA4 was also distributed among hypertrophic chondrocytes and osteoblasts in the growth plate of developing mouse long bones. In vitro evaluation revealed that ephA4 expression was elevated upon hypertrophic differentiation of chondrocytes and that markedly stronger expression was observed in osteoblastic SaOS-2 than chondrocytic HCS-2/8 cells. Of note, RNAi-mediated silencing of ephA4 in SaOS-2 cells resulted in the repression of osteocalcin gene expression and alkaline phosphatase activity. Interestingly, confocal laser-scanning microscopic analysis revealed the presence of EphA4 molecules in the nucleus as well as on the surface of SaOS-2 cells. These findings are the first indication of a critical role of EphA4 in ossification, especially at the final stage in which osteoblasts and hypertrophic chondrocytes play major roles. Biochemical and biophysical research communications 374 1 22 7 20080912 10.1016/j.bbrc.2008.06.089 18601903 miho.inoue/published_papers/51032178 Mitsuhiro Hoshijima Takako Hattori Miho Inoue Daisuke Araki Hiroshi Hanagata Akira Miyauchi Masaharu Takigawa CT domain of CCN2/CTGF directly interacts with fibronectin and enhances cell adhesion of chondrocytes through integrin alpha5beta1. Searching for CCN family protein 2/connective tissue growth factor (CCN2/CTGF) interactive proteins by yeast-two-hybrid screening, we identified fibronectin 1 gene product as a major binding partner of CCN2/CTGF in the chondrosarcoma-derived chondrocytic cell line HCS-2/8. Only the CT domain of CCN2/CTGF bound directly to fibronectin (FN). CCN2/CTGF and its CT domain enhanced the adhesion of HCS-2/8 cells to FN in a dose-dependent manner. The CCN2/CTGF-enhancing effect on cell adhesion to FN was abolished by a blocking antibody against alpha5beta1 integrin (alpha5beta1), but not by one against anti-alphavbeta3 integrin. These findings suggest for the first time that CCN2/CTGF enhances chondrocyte adhesion to FN through direct interaction of its C-terminal CT domain with FN, and that alpha5beta1 is involved in this adhesion. FEBS letters 0014-5793 580 5 1376 82 20060220 16457822 miho.inoue/published_papers/51032177 Ronald G Craig Angela R Kamer Sathya P Kallur Miho Inoue Dennis P Tarnow Effects of periodontal cell grafts and enamel matrix proteins on the implant-connective tissue interface: a pilot study in the minipig. We have developed an experimental model to help identify and characterize factors necessary for periodontal connective tissue attachment formation on dental implants. In this pilot study, we report the effect of autogenous periodontal cell grafts, with and without the a pplication of enamel matrix derivative (EMD), on the implant-connective tissue interface. Periodontal ligament (PDL) and gingival connective tissue (GCT) cultures were established from an adult minipig. Implants were placed in osteotomies prepared with exaggerated countersinks that served as recipient sites for autogenous cell grafts in bilateral edentulated posterior mandibular sextants. In addition, 1 side received an application of EMD before placement of the autogenous cell grafts. A bioabsorbable membrane covering the coronal portion of the implants was placed before closure. After 8 weeks, quantitative histomorphometric and qualitative light microscopic analyses revealed that the implants that received gelatin vehicle alone were surrounded by bone, whereas the implants that received GCT cell grafts were mostly surrounded by fibrous connective tissue. In contrast, implants that received PDL cells without the application of EMD demonstrated good bone contact, but strands of epithelium were observed in the implant-connective tissue interface. Implants that received PDL cells and EMD also had good bone contact but without evidence of epithelium. A cementum-like interface was not observed in any of the groups. Results of this pilot study suggest that EMD and the type of cell populations present in the implant wound-healing environment may alter the implant-connective tissue interface. The Journal of oral implantology 0160-6972 32 5 228 36 20060000 17069167 miho.inoue/published_papers/51032181 Hidetsugu Tsujigiwa Hitoshi Nagatsuka Mehmet Gunduz Andrea Rodriguez Rosario S Rivera Racquel Z Legeros Miho Inoue Noriyuki Nagai Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells. The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features, and biocompatibility, is an effective carrier for rhBMP-2. However, the conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone-induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization; (2) studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated atelocollagen in augmenting cellular activity. Journal of biomedical materials research. Part A 1549-3296 75 1 210 5 20051001 16059889 miho.inoue/published_papers/51032179 Miho Inoue Hitoshi Nagatsuka Hidetsugu Tsujigiwa Masahisa Inoue Racquel Z LeGeros Toshio Yamamoto Noriyuki Nagai In vivo effect of fluoride-substituted apatite on rat bone. Different types of calcium phosphate compounds are commercially available for medical and dental applications as bone substitute materials. Biological apatites contain several kinds of minor elements such as carbonate (CO3), magnesium (Mg), and fluoride (F) in enamel, dentin, and bone. It has been shown that F ion and F-substituted apatite promoted osteoblast proliferation and inhibited osteoclast cell activity. The purpose of this study was to investigate the in vivo rat tibia activity on F-substituted apatite (FAp). Apatites of unsintered calcium deficient apatite (CDA), and FAps, with low, medium, and high F concentrations, were implanted in rat tibia for 1 and 2 weeks. Implanted tissues were embedded in paraffin blocks, stained with hematoxylin-eosin and histomorphometrically observed. Results showed that low F concentration induced better and faster new bone formation in vivo compared to CDA. Therefore the results suggested that F as a minor element in bone rendered a suitable effect on bone formation in vivo. Dental materials journal 0287-4547 24 3 398 402 20050900 16279730 miho.inoue/published_papers/51032180 Hiroaki Nakamura Noriyuki Nagaoka Azumi Hirata Miho Inoue Hidehiro Ozawa Toshio Yamamoto Distribution of actin filaments, non-muscle myosin, M-Ras, and extracellular signal-regulated kinase (ERK) in osteoclasts after calcitonin administration. Scanning electron microscopy (SEM) was employed to study the effect of calcitonin on the distribution of actin filaments in osteoclasts obtained from rat tibiae. Fluorescent microscopy was also applied to examine calcitonin-induced changes in the distribution of actin filaments, non-muscle myosin, M-Ras, and extracellular signal-regulated kinase (ERK) to clarify the role of ERK in the cytoskeleton of osteoclasts. SEM of control osteoclasts revealed a ring-like structure in the peripheral region. Labeled actin filaments and non-muscle myosin were detected in the peripheral region and exhibited a ring-like pattern. Immunoreactivity indicating M-Ras and ERK was also detected in the vicinity of the actin ring. After calcitonin treatment, many osteoclasts exhibited a retracted appearance and lacked a discernible actin ring. Numerous retraction fibers were found at the edge of calcitonin-treated osteoclasts. Actin filaments and non-muscle myosin were concentrated in the cytoplasm of calcitonin-treated osteoclasts, and exhibited a filamentous pattern. Labeled M-Ras and ERK also accumulated in the central region of these osteoclasts. These findings suggest that actin-myosin interaction plays an essential role in the retraction of osteoclasts induced by calcitonin. ERK may play a role in this interaction by activating myosin light chain kinase, as previously observed in smooth muscle cells. Archives of histology and cytology 0914-9465 68 2 143 50 20050600 16079460 miho.inoue/published_papers/51032183 Hiroaki Nakamura Ryoko Kato Azumi Hirata Miho Inoue Toshio Yamamoto Localization of CD44 (hyaluronan receptor) and hyaluronan in rat mandibular condyle. CD44 is a multifunctional adhesion molecule that binds to hyaluronan (HA), type I collagen, and fibronectin. We investigated localization of CD44 and HA in mandibular condylar cartilage compared with the growth plate and the articular cartilage, to clarify the characteristics of chondrocytes. We also performed Western blotting using a lysate of mandibular condyle. In mandibular condyle, CD44-positive cells were seen in the surface region of the fibrous cell layer and in the proliferative cell layer. Western blotting revealed that the molecular weight of CD44 in condyle was 78 to 86 kD. Intense reactivity for HA was detected on the surface of the condyle and the lacunae of the hypertrophic cell layer. Moderate labeling was seen in cartilage matrix of the proliferative and maturative layer. Weak labeling was also seen in the fibrous cell layer. In growth plate and articular cartilage, HA was detected in all cell layers. However, chondrocytes of these cartilages did not exhibit reactivity for CD44. These results suggest that chondrocytes in the mandibular condylar cartilage differ in expression of CD44 from those in tibial growth plate and articular cartilage. Cell-matrix interaction between CD44 and HA may play an important role in the proliferation of chondrocytes in the mandibular condyle. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 0022-1554 53 1 113 20 20050100 15637344 miho.inoue/published_papers/51032182 Toshio Yamamoto Noriyuki Nagaoka Azumi Hirata Hiroaki Nakamura Miho Inoue Mariko Kawai Mika Ikegame Ultrastructural and immunohistochemical studies of medullary bone calcification, with special reference to sulphated glycosaminoglycans. Histochemical, immunohistochemical and electron energy-loss spectroscopic studies were performed to examine the relationship between sulphated glycosaminoglycans and medullary bone calcification using oestrogen-injected male Japanese quail. Sulphated glycosaminoglycans, detected by high iron diamine (HID) or HID-thiocarbohydrazide-silver protein (HID-TCH-SP) methods, were distributed throughout the matrix of medullary bone, some periphery and extending tips of the trabeculae stained weakly, and the globular structures at osteoid areas were exclusively positive for HID-TCH-SP stain. Immunohistochemistry identified keratan sulphate located in the globular structures at osteoid areas and calcified matrix, but chondroitin-4 sulphate and chondroitin-6 sulphate were not detected in the matrix. Using electron spectroscopic imaging, sulphur was determined to be localized in the globular structures. These results demonstrate that medullary bone matrix accumulates keratan sulphate in the globular structures, which are the foci for calcification, and eventually in the calcified areas. This suggests that keratan sulphate containing sulphur is maintained in the calcified matrix. These results indicate a unique process of calcification exists in medullary bone. Journal of electron microscopy 0022-0744 54 1 29 34 20050100 15695482 miho.inoue/published_papers/51032184 Miho Inoue Racquel Z LeGeros Masahisa Inoue Hidetsugu Tsujigiwa Hitoshi Nagatsuka Toshio Yamamoto Noriyuki Nagai In vitro response of osteoblast-like and odontoblast-like cells to unsubstituted and substituted apatites. Different types of calcium phosphate compounds [calcium-deficient apatite (CDA); beta-tricalcium phosphate (beta-TCP); biphasic calcium phosphate (BCP)] are commercially available for medical and dental applications as bone substitute materials. Most of the reported in vitro studies on cell-material interactions have used osteoblast-like cells. The purpose of this study was to investigate the in vitro response of osteoblast-like (MC3T3-E1) and odontoblast-like (MDPC23) cells on unsubstituted (HA) and substituted (F-substituted) apatites. MC3T3-E1 and MDPC23 were cultured in alpha-modified medium containing 10% fetal bovine serum, ascorbic acid (50 microg/mL) and beta-glycerophosphate (2 mM). The cells were seeded on pellets made from HA, and FAp (with low, medium, and high F concentrations). Cell morphology was observed after 7 and 14 days using scanning electron microscopy (SEM). Cell attachment and differentiation were determined from the DNA content, alkaline phosphatase (ALP) activity, and total collagen content. Pellet surface composition was characterized by using Fourier Transform infrared spectroscopy. MC3T3-E1 and MDPC23 cells on HA were normal in shape and in fusion but not on FAp. Results of this study showed that the pattern of cell proliferation of osteoblast-like cells was different from that of the odontoblast-like cells. This study suggests that cell morphology, fusion, and proliferation on biomaterial surfaces depend on cell type (osteoblast-like vs odontoblast-like cell) and biomaterial composition (unsubstituted vs substituted F-apatites). Journal of biomedical materials research. Part A 1549-3296 70 4 585 93 20040915 15307163 miho.inoue/published_papers/51032185 Kumiko Nawachi Miho Inoue Satoshi Kubota Takashi Nishida Gen Yosimichi Tohru Nakanishi Manabu Kanyama Takuo Kuboki Hirofumi Yatani Tomoichiro Yamaai Masaharu Takigawa Tyrosine kinase-type receptor ErbB4 in chondrocytes: interaction with connective tissue growth factor and distribution in cartilage. In order to identify receptor molecules that participate in the growth and differentiation of chondrocytes, we cloned a number of cDNA fragments from HCS-2/8 chondrocytic cells, by using tyrosine kinase-specific primers for amplification. The mRNA expression of one such receptor, ErbB4, was increased by connective tissue growth factor/hypertrophic chondrocyte-specific gene product (CTGF/Hcs24), which promotes all stages of the endochondral ossification in vitro. ErbB4 expression was observed through all stages of chondrocytic differentiation in vitro, corresponding to the wide distribution of CTGF/Hcs24 target cells. Furthermore, positive signals for erbB4 mRNA were detectable throughout most populations of chondrocytes, in growth and articular cartilage in vivo. These results demonstrate for the first time that ErbB4 is expressed in chondrocytes and may play some roles in chondrocytic growth and differentiation along with CTGF/Hcs24. FEBS letters 0014-5793 528 1-3 109 13 20020925 12297288 miho.inoue/misc/54155634 武川大輔 鈴木善貴 鴨居浩平 小池一幸 大倉一夫 小澤彩 柴垣あかり 井上美穂 保坂啓一 松香芳三 Subjective assessment of 3D-printed music splint (MuS) shapes for woodwind players 3Dプリンターを用いて製作した木管奏者用ミュージックスプリントの形状別主観評価 日本演奏芸術医学会学術集会抄録集(Web) 4th null null 20250000 miho.inoue/misc/50877682 井上美穂 鈴木善貴 山田輝乃 井上正久 松香芳三 Effect of aromatherapy on sleep bruxism: A randomized double-blind crossover controlled tria 睡眠時ブラキシズムに対するアロマテラピーの効果-無作為化二重盲検クロスオーバー比較試験- アロマテラピー学雑誌(Web) 2189-5147 26 1 null null 20250000 miho.inoue/misc/50877689 新開瑞希 鈴木善貴 大倉一夫 小澤彩 柴垣あかり 谷脇竜弥 井上美穂 松香芳三 口腔粘膜湿潤度および唾液分泌量と睡眠時ブラキシズムの関連の調査 睡眠口腔医学 2188-6695 11 null null 20240000 miho.inoue/misc/50877688 井上美穂 鈴木善貴 新開瑞希 谷脇竜弥 大倉一夫 松香芳三 睡眠時ブラキシズムに対する各種精油の効果 睡眠口腔医学 2188-6695 11 null null 20240000 miho.inoue/misc/50877687 谷脇竜弥 大倉一夫 鈴木善貴 井上美穂 新開瑞希 松香芳三 糖質制限による睡眠時ブラキシズムへの効果の検討,中間報告 睡眠口腔医学 2188-6695 11 null null 20240000 miho.inoue/misc/50877686 小澤彩 鈴木善貴 大倉一夫 田島登誉子 谷脇竜弥 新開瑞希 柴垣あかり 井上美穂 大川敏永 安陪晋 重本修伺 松香芳三 Does sleep bruxism deform the occlusal appliance? 睡眠時ブラキシズムはオクルーザルアプライアンスを変形させるのか 日本補綴歯科学会誌(Web) 1883-6860 16 null null 20240000 miho.inoue/misc/50877685 谷脇竜弥 細木真紀 宮城麻友 板東伸幸 大島正充 鈴木善貴 井上美穂 生田目大介 小澤彩 青木映璃 吉原靖智 大倉一夫 新開瑞希 柴垣あかり 松香芳三 Improving crown-bridge prosthetics practice: Analysis based on a questionnaire survey クラウンブリッジ補綴学模型実習の改善:アンケート調査に基づく分析 日本補綴歯科学会誌(Web) 1883-6860 16 null null 20240000 miho.inoue/misc/50877684 井上正久 岡本果鈴 川上隆茂 阿部友美 瀬津弘順 井上美穂 細木眞紀 松香芳三 Immunohistochemical study of eosinophil localization in titanium- and nickel- induced allergic mice チタン及びニッケル誘発アレルギーマウスにおける好酸球局在の免疫組織化学的検討 硬組織再生生物学会学術大会・総会プログラム・抄録集 32nd null null 20240000 miho.inoue/misc/50877683 小池一幸 細木眞紀 田島登誉子 宮城麻友 井上美穂 生田目大介 吉原靖智 谷脇竜弥 大島正充 松香芳三 Pustulotic arthro-osteitis: a case report examining the significant role of dental metal removal 掌蹠膿疱症性骨関節炎における歯科金属除去の重要性:症例報告と考察 日本補綴歯科学会中国・四国支部学術大会プログラム・抄録集(Web) 2024 null null 20240000 miho.inoue/misc/52725441 青木映璃 鈴木善貴 新開瑞希 小澤彩 柴垣あかり 谷脇竜弥 吉原靖智 大倉一夫 井上美穂 池田隆志 大島正充 松香芳三 Investigation of Effect of Sleep Positional Therapy on Sleep Bruxism 睡眠体位療法による睡眠時ブラキシズムへの効果の検討 日本補綴歯科学会中国・四国支部学術大会プログラム・抄録集(Web) 2023 null null 20230000 miho.inoue/misc/50877691 細木真紀 生田目大介 宮城麻友 大倉一夫 井上美穂 小澤彩 柴垣あかり 谷脇竜弥 鴨居浩平 板東伸幸 吉原靖智 大島正充 鈴木善貴 新開瑞希 松香芳三 Study of crown-bridge prosthodontics practice in response to digitalization デジタル化に対応したクラウンブリッジ補綴学実習の検討 日本補綴歯科学会誌(Web) 1883-6860 15 null null 20230000 miho.inoue/misc/50877690 小澤彩 鈴木善貴 田島登誉子 大川敏永 鴨居浩平 大倉一夫 谷脇竜弥 井上美穂 吉原靖智 大島正充 安陪晋 松香芳三 Observed deformation of an appliance fitted to a patient with sleep braxism. 睡眠時ブラキシズム患者に装着したアプライアンスの変形を観察した症例 日本補綴歯科学会誌(Web) 1883-6860 15 null null 20230000 miho.inoue/misc/52725447 新開瑞希 細木真紀 鈴木善貴 田島登誉子 井上美穂 小澤彩 吉原靖智 大島正充 織田英正 本田常晴 松香芳三 Risk of Resin Allergy and Appropriate Dental Treatment レジンアレルギーのリスクと歯科治療における対応 日本補綴歯科学会誌(Web) 1883-6860 14 null null 20220000 miho.inoue/misc/52725456 吉原靖智 鈴木善貴 大倉一夫 重本修司 安陪晋 大島正充 細木真紀 井上美穂 坂東永一 松香芳三 A Case of Three-Dimensional Jaw Movement analysis of RMMA with Jaw Movement 顎運動を伴うRMMAの三次元顎運動解析を行った症例 日本補綴歯科学会誌(Web) 1883-6860 13 null null 20210000 miho.inoue/misc/51032436 松香 芳三 大島 正充 大倉 一夫 細木 真紀 鈴木 善貴 宮城 麻友 井上 美穂 インドネシアの歯学部における学部教育—Undergraduate Student Education at Dental Schools in Indonesia 東京 : 日本口腔リハビリテーション学会事務局 日本口腔リハビリテーション学会雑誌 = The journal of Japan Association of Oral Rehabilitation 2186-2052 33 1 55 59 20200000 1520572359516377600 miho.inoue/misc/51032437 大倉 一夫 鈴木 善貴 重本 修伺 大本 勝弘 葉山 莉香 岩浅 匠真 成谷 美緒 井上 美穂 上枝 麻友 安陪 晋 松香 芳三 スプリントによる睡眠時ブラキシズムに対する治療効果 : 予備的検討—The effect of splints on sleep bruxism : a preliminary study 東京 : 日本口腔リハビリテーション学会事務局 日本口腔リハビリテーション学会雑誌 = The journal of Japan Association of Oral Rehabilitation 2186-2052 29 1 21 27 20160000 1520572359226098048 miho.inoue/misc/51032433 山合 友一朗 中西 徹 井上 美穂 杉本 朋貞 滝川 正春 CTGF,ErbB受容体遺伝子発現の，Cbfal遺伝子ノックアウトによる影響 歯科基礎医学会 1349-0079 46 5 467 null 20040901 1570854177450353664 miho.inoue/misc/51032435 山合 友一朗 中西 徹. 縄稚 久美子 井上 美穂 杉本 朋貞 滝川 正春. ErbB2のノックアウトマウスにおける肥大軟骨のアポトーシス 歯科基礎医学会 歯科基礎医学会雑誌 0385-0137 45 5 320 null 20030901 1574231877171118976 miho.inoue/misc/51032431 平田 あずみ 中村 浩彰 井上 美穂 山本 敏男 セメント質形成におけるRunx2陽性細胞と基質タンパクの局在 歯科基礎医学会 歯科基礎医学会雑誌 0385-0137 45 5 289 null 20030901 1571980077356280448 miho.inoue/misc/51032430 中村 浩彰 平田 あずみ 井上 美穂 山本 敏男 エルカトニンによる破骨細胞の細胞骨格変化 歯科基礎医学会 歯科基礎医学会雑誌 0385-0137 45 5 340 null 20030901 1574231877170006016 miho.inoue/misc/51032434 山合 友一朗 中西 徹 縄稚 久美子 井上 美穂 杉本 朋貞 滝川 正春 硬組織発生過程でのerbB4遺伝子発現 歯科基礎医学会 歯科基礎医学会雑誌 0385-0137 44 5 435 null 20020920 1572543027310781312 miho.inoue/misc/51032479 OZAKI M. 尾崎 美穂 SHIMOHARAI K. 下佛 幸治 MURAKAMI Y. 村上 泰弘 KATO H. 加藤 久晴 ICHINOSE Y. 一瀬 勇規 SHIRAISHI T. 白石 友紀 YAMADA T. 山田 哲治 Regulation of Pea PAL Gene Expression in Transgenic Plants (116) 形質転換植物におけるエンドウPAL遺伝子の発現制御機構 (日本植物病理大会) The Phytopathological Society of Japan (PSJ) 日本植物病理学会 Annals of the Phytopathological Society of Japan 日本植物病理學會報 0031-9473 61 3 238 null 19950625 1571980077110741376