Kohji/published_papers/54219435 Kohji Yamada No energy, no defense: Metabolic input shapes defense signaling Elsevier BV Current Opinion in Plant Biology 1369-5266 92 102920 102920 20260800 10.1016/j.pbi.2026.102920 Kohji/published_papers/51798616 Linnan Jie Miho Sanagi Shigetaka Yasuda Kohji Yamada Saki Ejima Ayumi Sugisaki Junpei Takagi Mika Nomoto Xiu-Fang Xin Yasuomi Tada Yusuke Saijo Takeo Sato Cellular energy sensor SnRK1 suppresses salicylic acid-dependent and -independent defenses and bacterial resistance in Arabidopsis. In nature, plants cope with various pathogens that compete for cellular resources during infection. It has long been suggested that plant defense activity must be linked to cellular energy and metabolic states to optimize the balance between growth and defense. However, the molecular mechanisms that regulate immune activity in relation to cellular energy status remain unclear. Here, we demonstrate that the plant energy sensor SNF1-RELATED KINASE 1 (SnRK1) plays a critical role in modulating defense responses and bacterial resistance in Arabidopsis thaliana. Bacterial elicitor-induced expression of defense marker genes, such as PATHOGENESIS-RELATED 1 (PR1), is significantly repressed under sugar-limited conditions in wild-type seedlings, whereas this expression is markedly enhanced in the snrk1α1i/α2 knockdown mutants. SnRK1 restricts defense-related gene expression and resistance to the biotrophic bacterial pathogen Pseudomonas syringae pv. tomato DC3000, which are partly dependent on salicylic acid (SA). In addition, we found that the SnRK1 kinase activity is increased by high humidity. Consistently, SnRK1 is critical for the suppression of SA-mediated defense responses under high humidity conditions. SnRK1 physically associates with the SA-related transcription factors TGACG SEQUENCE-SPECIFIC BINDING PROTEIN 4 (TGA4) and TGA2 to attenuate PR1 expression. These findings provide valuable insight into the molecular mechanisms linking cellular energy status with immune regulation in plants. Proceedings of the National Academy of Sciences of the United States of America 122 48 e2527765122 null 20251202 10.1073/pnas.2527765122 41296736 Kohji/published_papers/50127621 山田晃嗣 糖を巡る植物‒病原菌間相互作用 生化学 97 2 231 235 20250400 10.14952/SEIKAGAKU.2025.970231 Kohji/published_papers/44516777 Kohji Yamada Akira Mine Sugar coordinates plant defense signaling. Pathogen recognition triggers energy-intensive defense systems. Although successful defense should depend on energy availability, how metabolic information is communicated to defense remains unclear. We show that sugar, especially glucose-6-phosphate (G6P), is critical in coordinating defense in Arabidopsis. Under sugar-sufficient conditions, phosphorylation levels of calcium-dependent protein kinase 5 (CPK5) are elevated by G6P-mediated suppression of protein phosphatases, enhancing defense responses before pathogen invasion. Subsequently, recognition of bacterial flagellin activates sugar transporters, leading to increased cellular G6P, which elicits CPK5-independent signaling promoting synthesis of the phytohormone salicylic acid (SA) for antibacterial defense. In contrast, while perception of fungal chitin does not promote sugar influx or SA accumulation, chitin-induced synthesis of the antifungal compound camalexin requires basal sugar influx activity. By monitoring sugar levels, plants determine defense levels and execute appropriate outputs against bacterial and fungal pathogens. Together, our findings provide a comprehensive view of the roles of sugar in defense. Science advances 10 4 eadk4131 null 20240126 10.1126/sciadv.adk4131 38266087 Kohji/published_papers/41805279 Yoshihiro Inoue Trinh Thi Phuong Vy Suthitar Singkaravanit-Ogawa Ru Zhang Kohji Yamada Taiki Ogawa Junya Ishizuka Yoshihiro Narusaka Yoshitaka Takano Selective deployment of virulence effectors correlates with host specificity in a fungal plant pathogen. The hemibiotrophic fungal plant pathogen Colletotrichum orbiculare is predicted to secrete hundreds of effector proteins when the pathogen infects cucurbit crops, such as cucumber and melon, and tobacco (Nicotiana benthamiana), a distantly related Solanaceae species. Here, we report the identification of sets of C. orbiculare effector genes that are differentially required for fungal virulence to two phylogenetically distant host species. Through targeted gene knockout screening of C. orbiculare 'core' effector candidates defined based on in planta gene expression, we identified: four host-specific virulence effectors (named effector proteins for cucurbit infection, or EPCs) that are required for full virulence of C. orbiculare to cucurbit hosts, but not to the Solanaceae host N. benthamiana; and five host-nonspecific virulence effectors, which collectively contribute to fungal virulence to both hosts. During host infection, only a small subset of genes, including the host-specific EPC effector genes, showed preferential expression on one of the hosts, while gene expression profiles of the majority of other genes, including the five host-nonspecific effector genes, were common to both hosts. This work suggests that C. orbiculare adopts a host-specific effector deployment strategy, in addition to general host-blind virulence mechanisms, for adaptation to cucurbit hosts. The New phytologist null null 20230320 10.1111/nph.18790 36939621 Kohji/published_papers/41304750 Kohji Yamada Toya Yamamoto Kanon Uwasa Keishi Osakabe Yoshitaka Takano The establishment of multiple knockout mutants of Colletotrichum orbiculare by CRISPR-Cas9 and Cre-loxP systems. Colletotrichum orbiculare is employed as a model fungus to analyze molecular aspects of plant-fungus interactions. Although gene disruption via homologous recombination (HR) was established for C. orbiculare, this approach is laborious due to its low efficiency. Here we developed methods to generate multiple knockout mutants of C. orbiculare efficiently. We first found that CRISPR-Cas9 system massively promoted gene-targeting efficiency. By transiently introducing a CRISPR-Cas9 vector, more than 90% of obtained transformants were knockout mutants. Furthermore, we optimized a self-excision Cre-loxP marker recycling system for C. orbiculare because a limited availability of desired selective markers hampers sequential gene disruption. In this system, the integrated selective marker is removable from the genome via Cre recombinase driven by a xylose-inducible promoter, enabling the reuse of the same selective marker for the next transformation. Using our CRISPR-Cas9 and Cre-loxP systems, we attempted to identify functional sugar transporters involved in fungal virulence. Multiple disruptions of putative quinate transporter genes restricted fungal growth on media containing quinate as a sole carbon source, confirming their functionality as quinate transporters. However, our analyses showed that quinate acquisition was dispensable for infection to host plants. In addition, we successfully built mutations of 17 cellobiose transporter genes in a strain. From the data of knockout mutants that we established in this study, we inferred that repetitive rounds of gene disruption using CRISPR-Cas9 and Cre-loxP systems do not cause adverse effects on fungal virulence and growth. Therefore, these systems will be powerful tools to perform a systematic loss-of-function approach for C. orbiculare. Fungal genetics and biology : FG & B 103777 103777 20230117 10.1016/j.fgb.2023.103777 36669556 Kohji/published_papers/37549824 Eliza P.-I. Loo Yuri Tajima Kohji Yamada Shota Kido Taishi Hirase Hirotaka Ariga Tadashi Fujiwara Keisuke Tanaka Teruaki Taji Imre E. Somssich Jane E. Parker Yusuke Saijo Recognition of Microbe- and Damage-Associated Molecular Patterns by Leucine-Rich Repeat Pattern Recognition Receptor Kinases Confers Salt Tolerance in Plants In plants, a first layer of inducible immunity is conferred by pattern recognition receptors (PRRs) that bind microbe- and damage-associated molecular patterns to activate pattern-triggered immunity (PTI). PTI is strengthened or followed by another potent form of immunity when intracellular receptors recognize pathogen effectors, termed effector-triggered immunity. Immunity signaling regulators have been reported to influence abiotic stress responses as well, yet the governing principles and mechanisms remain ambiguous. Here, we report that PRRs of a leucine-rich repeat ectodomain also confer salt tolerance in Arabidopsis thaliana, following recognition of cognate ligands such as bacterial flagellin (flg22 epitope) and elongation factor Tu (elf18 epitope), and the endogenous Pep peptides. Pattern-triggered salt tolerance (PTST) requires authentic PTI signaling components; namely, the PRR-associated kinases BAK1 and BIK1 and the NADPH oxidase RBOHD. Exposure to salt stress induces the release of Pep precursors, pointing to the involvement of the endogenous immunogenic peptides in developing plant tolerance to high salinity. Transcriptome profiling reveals an inventory of PTST target genes, which increase or acquire salt responsiveness following a preexposure to immunogenic patterns. In good accordance, plants challenged with nonpathogenic bacteria also acquired salt tolerance in a manner dependent on PRRs. Our findings provide insight into signaling plasticity underlying biotic or abiotic stress cross-tolerance in plants conferred by PRRs. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license . Scientific Societies Molecular Plant-Microbe Interactions® 1943-7706 35 7 554 566 20220700 10.1094/mpmi-07-21-0185-fi 34726476 Kohji/published_papers/34867579 Chihiro Abe-Hara Kohji Yamada Naoki Wada Risa Ueta Ryosuke Hashimoto Keishi Osakabe Yuriko Osakabe Effects of the sliaa9 Mutation on Shoot Elongation Growth of Tomato Cultivars. Tomato INDOLE-3-ACETIC ACID9 (SlIAA9) is a transcriptional repressor in auxin signal transduction, and SlIAA9 knockout tomato plants develop parthenocarpic fruits without fertilization. We generated sliaa9 mutants with parthenocarpy in several commercial tomato cultivars (Moneymaker, Rio Grande, and Ailsa Craig) using CRISPR-Cas9, and null-segregant lines in the T1 generation were isolated by self-pollination, which was confirmed by PCR and Southern blot analysis. We then estimated shoot growth phenotypes of the mutant plants under different light (low and normal) conditions. The shoot length of sliaa9 plants in Moneymaker and Rio Grande was smaller than those of wild-type cultivars in low light conditions, whereas there was not clear difference between the mutant of Ailsa Craig and the wild-type under both light conditions. Furthermore, young seedlings in Rio Grande exhibited shade avoidance response in hypocotyl growth, in which the hypocotyl lengths were increased in low light conditions, and sliaa9 mutant seedlings of Ailsa Craig exhibited enhanced responses in this phenotype. Fruit production and growth rates were similar among the sliaa9 mutant tomato cultivars. These results suggest that control mechanisms involved in the interaction of AUX/IAA9 and lights condition in elongation growth differ among commercial tomato cultivars. Frontiers in plant science 12 627832 627832 20210000 10.3389/fpls.2021.627832 34093603 Kohji/published_papers/43304590 Eliza P. Loo Yuri Tajima Kohji Yamada Taishi Hirase Hirotaka Ariga Tadashi Fujiwara Keisuke Tanaka Teruaki Taji Imre E. Somssich Jane E. Parker Yusuke Saijo Pattern recognition receptors confer plant salt tolerance via WRKY18/WRKY40 transcription factors <title>Abstract</title>Pattern recognition receptors (PRRs) bind microbe- and damage-associated molecular patterns (MAMPs/DAMPs, respectively) to enhance host immunity in animals and plants. Here, we report that PRRs also confer salt tolerance in the model plant <italic>Arabidopsis thaliana</italic> following recognition of cognate ligands, such as bacterial flagellin and EF-Tu and the endogenous Pep peptides. Pattern-triggered salt tolerance (PTST) requires the PRR-associated kinases <italic>BAK1</italic> and <italic>BIK1</italic>, and the NADPH oxidase <italic>RBOHD</italic>. Transcriptome profiling reveals an inventory of PTST target genes, which increase or acquire salt responsiveness following an exposure to immunogenic patterns. In their regulatory DNA sequences, specific binding sites for a subset of WRKY transcription factors are over-represented. Accordingly, PTST requires <italic>WRKY40</italic> and <italic>WRKY18</italic>, which activate salt tolerance-related genes but attenuate pathogen defense-related genes, including the <italic>EDS1</italic> immunity activator. PRR signaling leads to sustained WRKY40/WRKY18 accumulation under salt stress and utilizes both <italic>WRKY</italic>s for salt tolerance. The PRR-WRKY40/WRKY18 module also confers salt tolerance after challenge with non-pathogenic bacteria. Our findings give molecular insight into signaling plasticity underlying biotic-abiotic stress cross-tolerance in plants conferred by PRRs. Cold Spring Harbor Laboratory null null 20200507 10.1101/2020.05.07.082172 Kohji/published_papers/18982636 Irieda H Inoue Y Mori M Yamada K Oshikawa Y Saitoh H Uemura A Terauchi R Kitakura S Kosaka A Singkaravanit-Ogawa S Takano Y Conserved fungal effector suppresses PAMP-triggered immunity by targeting plant immune kinases. Plant pathogens have optimized their own effector sets to adapt to their hosts. However, certain effectors, regarded as core effectors, are conserved among various pathogens, and may therefore play an important and common role in pathogen virulence. We report here that the widely distributed fungal effector NIS1 targets host immune components that transmit signaling from pattern recognition receptors (PRRs) in plants. NIS1 from two Colletotrichum spp. suppressed the hypersensitive response and oxidative burst, both of which are induced by pathogen-derived molecules, in Nicotiana benthamianaMagnaporthe oryzae NIS1 also suppressed the two defense responses, although this pathogen likely acquired the NIS1 gene via horizontal transfer from Basidiomycota. Interestingly, the root endophyte Colletotrichum tofieldiae also possesses a NIS1 homolog that can suppress the oxidative burst in N. benthamiana We show that NIS1 of multiple pathogens commonly interacts with the PRR-associated kinases BAK1 and BIK1, thereby inhibiting their kinase activities and the BIK1-NADPH oxidase interaction. Furthermore, mutations in the NIS1-targeting proteins, i.e., BAK1 and BIK1, in Arabidopsis thaliana also resulted in reduced immunity to Colletotrichum fungi. Finally, M. oryzae lacking NIS1 displayed significantly reduced virulence on rice and barley, its hosts. Our study therefore reveals that a broad range of filamentous fungi maintain and utilize the core effector NIS1 to establish infection in their host plants and perhaps also beneficial interactions, by targeting conserved and central PRR-associated kinases that are also known to be targeted by bacterial effectors. Proceedings of the National Academy of Sciences of the United States of America 0027-8424 116 2 496 505 20190100 10.1073/pnas.1807297116 30584105 Kohji/published_papers/19431824 Kohji Yamada Yuriko Osakabe Sugar compartmentation as an environmental stress adaptation strategy in plants. The sessile nature of plants has driven their evolution to cope flexibly with ever-changing surrounding environments. The development of stress tolerance traits is complex, and a broad range of cellular processes are involved. Recent studies have revealed that sugar transporters contribute to environmental stress tolerance in plants, suggesting that sugar flow is dynamically fluctuated towards optimization of cellular conditions in adverse environments. Here, we highlight sugar compartmentation mediated by sugar transporters as an adaptation strategy against biotic and abiotic stresses. Competition for sugars between host plants and pathogens shapes their evolutionary arms race. Pathogens, which rely on host-derived carbon, manipulate plant sugar transporters to access sugars easily, while plants sequester sugars from pathogens by enhancing sugar uptake activity. Furthermore, we discuss pathogen tactics to circumvent sugar competition with host plants. Sugar transporters also play a role in abiotic stress tolerance. Exposure to abiotic stresses such as cold or drought stress induces sugar accumulation in various plants. We also discuss how plants allocate sugars under such conditions. Collectively, these findings are relevant to basic plant biology as well as potential applications in agriculture, and provide opportunities to improve crop yield for a growing population. Seminars in cell & developmental biology 1084-9521 83 106 114 20181100 10.1016/j.semcdb.2017.12.015 29287835 Kohji/published_papers/19301330 Kohji Yamada Yuriko Osakabe Kazuko Yamaguchi-Shinozaki A C-terminal motif contributes to the plasma membrane localization of Arabidopsis STP transporters PLOS ONE 1932-6203 12 10 e0186326 null 20171000 10.1371/journal.pone.0186326 29028820 Kohji/published_papers/25748492 高野義孝 入枝泰樹 Suthitar Singkaravanit-Ogawa Nur Sabrina Ahmad Azmi 山田晃嗣 奥田竜太 石塚隼也 田中左恵子 井上喜博 炭疽病菌の植物感染戦略とエフェクター: 繋がる・繋げる・植物感染生理ネットワーク 日本植物病理学会植物感染生理談話会論文集 52 53 62 20170700 Kohji/published_papers/19301331 Kohji Yamada Yusuke Saijo Hirofumi Nakagami Yoshitaka Takano Regulation of sugar transporter activity for antibacterial defense in Arabidopsis SCIENCE 1095-9203 354 6318 1427 1430 20161200 10.1126/science.aah5692 27884939 Kohji/published_papers/19301332 Kohji Yamada Misuzu Yamashita-Yamada Taishi Hirase Tadashi Fujiwara Kenichi Tsuda Kei Hiruma Yusuke Saijo Danger peptide receptor signaling in plants ensures basal immunity upon pathogen-induced depletion of BAK1 EMBO JOURNAL 1460-2075 35 1 46 61 20160100 10.15252/embj.201591807 26574534 Kohji/published_papers/25358157 Saijo Y Yamada K Fine control of plant immunity through recognition of danger-associated molecular patterns. In nature, plants live with a wide range of microbes that reside on the surface of or within plant tissues. Plants disregard or tolerate these nonpathogenic microbes, but mount inducible defenses when they encounter potentially infectious microbes. The danger hypothesis predicts that a plant can sense and respond to danger/damage-associated molecular patterns (DAMPs) associated with pathogen challenge in addition to the microbe-associated molecular patterns (MAMPs) that are largely shared by all microbes. Here we provide an overview of recent studies on DAMP sensing and signaling in plant immunity. We also introduce our studies pointing to the importance of layered crosstalk between MAMP and DAMP signaling pathways as a critical step in basal resistance and systemic acquired resistance. The Phytopathological Society of Japan 日本植物病理学会 Japan J Phytopathol 0031-9473 81 4 322 331 20150000 10.3186/jjphytopath.81.322 Kohji/published_papers/19301333 Annegret Ross Kohji Yamada Kei Hiruma Misuzu Yamashita-Yamada Xunli Lu Yoshitaka Takano Kenichi Tsuda Yusuke Saijo The Arabidopsis PEPR pathway couples local and systemic plant immunity EMBO JOURNAL 1460-2075 33 1 62 75 20140100 10.1002/embj.201284303 24357608 Kohji/published_papers/19301334 Nico Tintor Annegret Ross Kazue Kanehara Kohji Yamada Li Fan Birgit Kemmerling Thorsten Nürnberger Kenichi Tsuda Yusuke Saijo Layered pattern receptor signaling via ethylene and endogenous elicitor peptides during Arabidopsis immunity to bacterial infection. Recognition of molecular patterns characteristic of microbes or altered-self leads to immune activation in multicellular eukaryotes. In Arabidopsis thaliana, the leucine-rich-repeat receptor kinases FLAGELLIN-SENSING2 (FLS2) and EF-TU RECEPTOR (EFR) recognize bacterial flagellin and elongation factor EF-Tu (and their elicitor-active epitopes flg22 and elf18), respectively. Likewise, PEP1 RECEPTOR1 (PEPR1) and PEPR2 recognize the elicitor-active Pep epitopes conserved in Arabidopsis ELICITOR PEPTIDE PRECURSORs (PROPEPs). Here we reveal that loss of ETHYLENE-INSENSITIVE2 (EIN2), a master signaling regulator of the phytohormone ethylene (ET), lowers sensitivity to both elf18 and flg22 in different defense-related outputs. Remarkably, in contrast to a large decrease in FLS2 expression, EFR expression and receptor accumulation remain unaffected in ein2 plants. Genome-wide transcriptome profiling has uncovered an inventory of EIN2-dependent and EFR-regulated genes. This dataset highlights important aspects of how ET modulates EFR-triggered immunity: the potentiation of salicylate-based immunity and the repression of a jasmonate-related branch. EFR requires ET signaling components for PROPEP2 activation but not for PROPEP3 activation, pointing to both ET-dependent and -independent engagement of the PEPR pathway during EFR-triggered immunity. Moreover, PEPR activation compensates the ein2 defects for a subset of EFR-regulated genes. Accordingly, ein2 pepr1 pepr2 plants exhibit additive defects in EFR-triggered antibacterial immunity, compared with ein2 or pepr1 pepr2 plants. Our findings suggest that the PEPR pathway not only mediates ET signaling but also compensates for its absence in enhancing plant immunity. Proceedings of the National Academy of Sciences of the United States of America 0027-8424 110 15 6211 6 20130409 10.1073/pnas.1216780110 23431187 Kohji/published_papers/19301335 Yuriko Osakabe Naoko Arinaga Taishi Umezawa Shogo Katsura Keita Nagamachi Hidenori Tanaka Haruka Ohiraki Kohji Yamada So-Uk Seo Mitsuru Abo Etsuro Yoshimura Kazuo Shinozaki Kazuko Yamaguchi-Shinozaki Osmotic Stress Responses and Plant Growth Controlled by Potassium Transporters in Arabidopsis PLANT CELL 1040-4651 25 2 609 624 20130200 10.1105/tpc.112.105700 23396830 Kohji/published_papers/19301336 Miki Fujita Yasunari Fujita Satoshi Iuchi Kohji Yamada Yuriko Kobayashi Kaoru Urano Masatomo Kobayashi Kazuko Yamaguchi-Shinozaki Kazuo Shinozaki Natural variation in a polyamine transporter determines paraquat tolerance in Arabidopsis PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 0027-8424 109 16 6343 6347 20120400 10.1073/pnas.1121406109 22492932 Kohji/published_papers/19301337 Mario Serrano Kazue Kanehara Martha Torres Kohji Yamada Nico Tintor Erich Kombrink Paul Schulze-Lefert Yusuke Saijo Repression of Sucrose/Ultraviolet B Light-Induced Flavonoid Accumulation in Microbe-Associated Molecular Pattern-Triggered Immunity in Arabidopsis PLANT PHYSIOLOGY 0032-0889 158 1 408 422 20120100 10.1104/pp.111.183459 22080602 Kohji/published_papers/19301338 Kohji Yamada Motoki Kanai Yuriko Osakabe Haruka Ohiraki Kazuo Shinozaki Kazuko Yamaguchi-Shinozaki Monosaccharide Absorption Activity of Arabidopsis Roots Depends on Expression Profiles of Transporter Genes under High Salinity Conditions JOURNAL OF BIOLOGICAL CHEMISTRY 0021-9258 286 50 43577 43586 20111200 10.1074/jbc.M111.269712 22041897 Kohji/published_papers/19301340 Kohji Yamada Yuriko Osakabe Junya Mizoi Kazuo Nakashima Yasunari Fujita Kazuo Shinozaki Kazuko Yamaguchi-Shinozaki Functional Analysis of an Arabidopsis thaliana Abiotic Stress-inducible Facilitated Diffusion Transporter for Monosaccharides JOURNAL OF BIOLOGICAL CHEMISTRY 0021-9258 285 2 1138 1146 20100100 10.1074/jbc.M109.054288 19901034 Kohji/published_papers/19301341 Yasunari Fujita Kazuo Nakashima Takuya Yoshida Takeshi Katagiri Satoshi Kidokoro Norihito Kanamori Taishi Umezawa Miki Fujita Kyonoshin Maruyama Kanako Ishiyama Masatomo Kobayashi Shoko Nakasone Kohji Yamada Takuya Ito Kazuo Shinozaki Kazuko Yamaguchi-Shinozaki Three SnRK2 Protein Kinases are the Main Positive Regulators of Abscisic Acid Signaling in Response to Water Stress in Arabidopsis PLANT AND CELL PHYSIOLOGY 1471-9053 50 12 2123 2132 20091200 10.1093/pcp/pcp147 19880399 Kohji/published_papers/19301339 Kohji Yamada Yuichiro Nakatsu Akio Onogi Junji Ueda Tomomasa Watanabe Specific Intracellular Localization and Antiviral Property of Genetic and Splicing Variants in Bovine Mx1 VIRAL IMMUNOLOGY 0882-8245 22 6 389 395 20091200 10.1089/vim.2009.0050 19951175 Kohji/published_papers/19301342 Kyonoshin Maruyama Migiwa Takeda Satoshi Kidokoro Kohji Yamada Yoh Sakuma Kaoru Urano Miki Fujita Kyouko Yoshiwara Satoko Matsukura Yoshihiko Morishita Ryosuke Sasaki Hideyuki Suzuki Kazuki Saito Daisuke Shibata Kazuo Shinozaki Kazuko Yamaguchi-Shinozaki Metabolic Pathways Involved in Cold Acclimation Identified by Integrated Analysis of Metabolites and Transcripts Regulated by DREB1A and DREB2A PLANT PHYSIOLOGY 1532-2548 150 4 1972 1980 20090800 10.1104/pp.109.135327 19502356 Kohji/published_papers/19301343 Kohji Yamada Yuichiro Nakatsu Akio Onogi Akiko Takasuga Yoshikazu Sugimoto Junji Ueda Tomomasa Watanabe Structural and Functional Analysis of the Bovine Mx1 Promoter JOURNAL OF INTERFERON AND CYTOKINE RESEARCH 1079-9907 29 4 217 226 20090400 10.1089/jir.2008.0069 19203250 Kohji/published_papers/19301349 H. A. E. Babiker Y. Nakatsu K. Yamada A. Yoneda A. Takada J. Ueda H. Hata T. Watanabe Bovine and water buffalo Mx2 genes: polymorphism and antiviral activity IMMUNOGENETICS 0093-7711 59 1 59 67 20070100 10.1007/s00251-006-0167-5 17119954 Kohji/published_papers/19301348 LH Ju A Onogi J Ueda K Yamada Y Nakatsu M Ohe H Hata K Sasaki T Watanabe Polymorphic study of equine antiviral MXA gene BIOCHEMICAL GENETICS 0006-2928 43 5-6 299 305 20050600 10.1007/s10528-005-5221-8 16144306 Kohji/published_papers/19301346 Y Nakatsu K Yamada J Ueda A Onogi GP Ables M Nishibori H Hata A Takada K Sawai Y Tanabe M Morita M Daikohara T Watanabe Genetic polymorphisms and antiviral activity in the bovine MX1 gene ANIMAL GENETICS 0268-9146 35 3 182 187 20040600 10.1111/j.1365-2052.2004.01125.x 15147388 Kohji/misc/52464012 河岡明義 橋本諒典 城所聡 山田晃嗣 刑部敬史 刑部祐里子 Functional analysis of Arabidopsis NADK2 gene in response to water stress 乾燥ストレスにおけるシロイヌナズナNADK2遺伝子の機能解析 日本植物生理学会年会(Web) 65th null null 20240000 Kohji/misc/52667040 宇津木一陽 安達凛奈 鳴坂義弘 鳴坂真理 山田晃嗣 刑部敬史 刑部祐里子 Functional analysis of SIGMA FACTOR-BINDING PROTEIN 1 in tomato トマト葉緑体シグマ因子相互作用タンパク質SIGMA FACTOR-BINDING PROTEIN1のゲノム編集技術による改変と機能解明 日本農芸化学会大会講演要旨集(Web) 2186-7976 2022 null null 20220000 Kohji/misc/52608955 奥田竜太 石塚隼也 SINGKARAVANIT-OGAWA Suthitar GAN Pamela 山田晃嗣 鳴坂義弘 白須賢 高野義孝 ウリ類炭疽病菌の付着器において発現するエフェクター候補群ECAPの機能解析 日本植物病理学会大会プログラム・講演要旨予稿集 2016 null null 20160000 Kohji/misc/48911828 刑部祐里子 桂彰吾 有永直子 長町啓太 田中秀典 山田晃嗣 徐劭旭 篠崎一雄 篠崎和子 シロイヌナズナにおけるカリウムトランスポーターKUP6を介した浸透圧ストレス応答と成長制御 日本植物生理学会年会要旨集 52nd 259 null 20110311 10.14841/jspp.2011.0.0590.0 Kohji/presentations/48383986 Linnan Jie Ayumi Sugisaki Shigetaka Yasuda 山田 晃嗣 Miho Sanagi Mika Nomoto Susumu Uehara Yasuomi Tada Yusuke Saijo Junpei Takagi Takeo Sato Analysis of SnRK1 functions in sugar responsive modulation of immunity in Arabidopsis 第65回日本植物生理学会 20240300 Kohji/presentations/48383977 山田 美鈴 峯 彰 山田 晃嗣 Identification of a novel defense component mediated by sugar signaling 第65回日本植物生理学会 20240300 Kohji/presentations/48384089 山田晃嗣 糖は免疫応答を制御する 第8回植物の栄養研究会 20230900 Kohji/presentations/48383999 山田 晃嗣 峯 彰 糖はプロテインキナーゼの活性化を介して防御応答を活性化させる 第64回日本植物生理学会 20230300 Kohji/presentations/48384013 山田 晃嗣 峯 彰 糖吸収は防御応答を増強させる 植物病理学会関西部会 20220900 Kohji/presentations/48384099 Kohji Yamada Sugar co-ordinates plant defense signaling 12th Japan-US Seminar in Plant Pathology 20220800 Kohji/presentations/48384027 宇津木 一陽 安達 凜奈 鳴坂 義弘 鳴坂 真理 山田 晃嗣 刑部 敬史 刑部 祐里子 トマト葉緑体シグマ因子相互作用タンパク質SIGMA FACTOR-BINDING PROTEIN 1のゲノム編集技術による改変と機能解明 日本農芸化学会2022年度大会 20220400 Kohji/presentations/48384037 山田 晃嗣 峯 彰 Sugar influx via transporters enhances defense signaling 第63回日本植物生理学会 20220300 Kohji/presentations/48384050 山田 晃嗣 防御応答と糖シグナルのクロストークの分子機構 第62回日本植物生理学会 20210300 Kohji/presentations/48384044 Ruoying Shi Ryota Sakai 山田 晃嗣 Paweł Bednarek Akira Abe Hiroaki Kato Kazuyuki Mise Ryohei Terauchi Yoshitaka Takano FIO1 is involved in inappropriate cell death in Arabidopsis nsl1 mutant 日本植物病理学会 20210300 Kohji/presentations/48384065 橋本 諒典 山田 晃嗣 刑部 敬史 刑部 祐里子 CRISPR/Cas9によるトマトNADキナーゼ2遺伝子の変異体作製と機能解析 第61回日本植物生理学会年会 20200300 Kohji/presentations/48384053 刑部 祐里子 橋本 諒典 宮城 敦子 澤田 有司 佐藤 心郎 山田 晃嗣 平井 優美 川合 真紀 刑部 敬史 乾燥ストレス応答における葉緑体局在性NADキナーゼ2の機能解析 第61回日本植物生理学会年会 20200300 Kohji/presentations/48384104 Kohji Yamada Sugar influx via transporters enhances defense signaling International Symposium on the Future Direction of Plant Science by Young Researchers 20191100 Kohji/presentations/48384147 Kohji Yamada Yoshitaka Takano Sugar transporters contribute to defense activation in Arabidopsis Molecular Plant-Microbe Interactions congress 20190700 Kohji/presentations/48384076 橋本 諒典 宮城 敦子 澤田 有司 佐藤 心郎 山田 晃嗣 平井 優美 川合 真紀 刑部 敬史 刑部 祐里子 植物非生物ストレスにおける葉緑体局在性NADキナーゼ遺伝子の機能解析 第60回日本植物生理学会年会 20190300 Kohji/presentations/48384072 島田 佳南里 井内 聖 井内 敦子 山田 晃嗣 刑部 敬史 刑部 祐里子 根毛形成に異常を示すシロイヌナズナ変異体の解析 第60回日本植物生理学会年会 20190300 Kohji/presentations/26541897 阿部千尋 上田梨紗 橋本諒典 山田晃嗣 刑部祐里子 刑部敬史 栽培品種トマトにおけるCRISPR/Cas9システムを用いた育種技術基盤の構築 日本植物生理学会年会(Web) 20180000 Kohji/presentations/26541886 島田佳南里 井内聖 井内敦子 山田晃嗣 刑部敬史 刑部祐里子 根毛形成に異常を示すシロイヌナズナ変異体の原因遺伝子の同定 日本植物生理学会年会(Web) 20180000 Kohji/presentations/26541909 阿部千尋 上田梨紗 橋本諒典 山田晃嗣 刑部祐里子 刑部敬史 栽培品種トマトにおけるCRISPR/Cas9システムを用いた育種技術基盤の構築 日本植物細胞分子生物学会大会・シンポジウム講演要旨集 20170820 Kohji/presentations/37279584 Yamada Kohji Saijo Yusuke Nakagami Hirofumi Takano Yoshitaka Regulation of sugar transporter activity for antibacterial defense in Arabidopsis Microbial pathogens strategically acquire metabolites from their hosts during infection. Here we show that the host can intervene to prevent such metabolite loss to pathogens. Phosphorylation-dependent regulation of sugar transporter 13 (STP13) is required for antibacterial defense in the plant Arabidopsis thaliana. STP13 physically associates with the flagellin receptor flagellin-sensitive 2 (FLS2) and its co-receptor BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1). BAK1 phosphorylates STP13 at threonine 485, which enhances its monosaccharide uptake activity to compete with bacteria for extracellular sugars. Limiting the availability of extracellular sugar deprives bacteria of an energy source and restricts virulence factor delivery. Our results reveal that control of sugar uptake, managed by regulation of a host sugar transporter, is a defense strategy deployed against microbial infection. Competition for sugar thus shapes host-pathogen interactions. American Association for the Advancement of Science 20161124 Kohji/presentations/34617859 入枝泰樹 入枝泰樹 森正之 山田晃嗣 押川友 大木進野 齋藤宏昌 寺内良平 高野義孝 糸状菌エフェクターNIS1は高等植物のPRR複合体における複数の因子を攻撃する 日本植物病理学会大会プログラム・講演要旨予稿集 20160000 Kohji/presentations/26541870 大開暖香 黒森崇 刑部祐里子 山田晃嗣 圓山恭之進 桂彰吾 長町啓太 篠崎一雄 篠崎和子 シロイヌナズナにおけるABCトランスポーターGサブファミリー遺伝子群の機能解析 日本植物生理学会年会要旨集 20130314 Kohji/presentations/26541867 刑部祐里子 有永直子 桂彰吾 長町啓太 田中秀典 山田晃嗣 SOUK Seo 梅澤泰史 安保充 吉村悦郎 篠崎一雄 篠崎和子 カリウムトランスポーターKUP6によるシロイヌナズナの浸透圧ストレス応答と生長制御 日本植物生理学会年会要旨集 20130314 Kohji/presentations/26541872 刑部祐里子 桂彰吾 有永直子 長町啓太 田中秀典 山田晃嗣 SOUK Seo 安保充 吉村悦郎 篠崎一雄 篠崎和子 シロイヌナズナにおけるカリウムトランスポーターKUP6を介した浸透圧ストレス応答と成長制御 日本植物生理学会年会要旨集 20120309 Kohji/presentations/26541871 大開暖香 黒森崇 刑部祐里子 山田晃嗣 桂彰吾 長町啓太 篠崎一雄 篠崎和子 シロイヌナズナのABCトランスポーターGサブファミリー遺伝子群の機能解析 日本植物生理学会年会要旨集 20120309 Kohji/presentations/26541875 刑部祐里子 桂彰吾 有永直子 長町啓太 田中秀典 山田晃嗣 徐劭旭 篠崎一雄 篠崎和子 シロイヌナズナにおけるカリウムトランスポーターKUP6を介した浸透圧ストレス応答と成長制御 日本植物生理学会年会要旨集 20110311 Kohji/presentations/26528752 山田晃嗣 中津祐一郎 小野木章雄 上田純治 渡辺智正 ウシ及びスイギュウMx1遺伝子の多様性と抗ウイルス活性に関する研究 日本畜産学会大会講演要旨 20050320 Kohji/presentations/34734040 瀬山智博 香川仁志 坂本晃子 山田晃嗣 五味花子 鈴木啓太 上田純治 渡辺智正 家禽のウイルス抵抗性Mx遺伝子の多様性に関する研究 日本畜産学会大会講演要旨 20050000 Kohji/presentations/26528757 菊林花 小野木章雄 上田純治 山田晃嗣 中津祐一郎 秦寛 佐々木一八 長谷川晃久 渡辺智正 ウマにおけるウイルス抵抗性MxA遺伝子の多型に関する研究 日本畜産学会大会講演要旨 20030915 Kohji/presentations/26528754 山田晃嗣 中津祐一郎 小野木章雄 上田純治 秦寛 田辺義弘 森田光男 渡辺智正 ウシMx遺伝子の多型性と抗ウイルス活性に関する研究 日本畜産学会大会講演要旨 20030915 Kohji/presentations/26528760 中津祐一郎 山田晃嗣 小野木章雄 上田純治 秦寛 GENE A 山内健治 田辺義弘 渡辺智正 ウシにおけるMx遺伝子の多型性に関する研究 日本畜産学会大会講演要旨 20030320 山田晃嗣 公益財団法人 康楽会 康楽賞 20210200 山田 晃嗣 徳島大学 徳島大学若手研究者学長表彰 20191200 山田 晃嗣 徳島県 徳島県科学技術大賞 若手研究者部門 20191000 山田 晃嗣 文部科学省 平成31年度 文部科学大臣表彰 若手科学者賞 20190400 Kohji Yamada 山田 晃嗣 The Japanease Society of Plant Physiologists 日本植物生理学会 JSPP Young Investigator Award 2018 第25回植物生理学会奨励賞 20180300 Kohji Yamada 山田 晃嗣 Japan Bioindustry Association バイオインダストリー協会 Bioindustry Research Award 2017 第一回バイオインダストリー奨励賞 20171000