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en: Proposals and evaluations for the enhancement of CAD/CAM education

en: Journal of Prosthodontic Research

ja: Journal of Prosthodontic Research


en: Bilateral transport of neuronal tracer after unilateral administration: Investigation of potential peripheral mechanism

en: Previous studies have shown pain attenuation after the administration of botulinum neurotoxin type A into the whisker pad contralateral to the nerve injury. It further showed localization in the neurons of the bilateral trigeminal ganglia, indicating a possible connection between the trigeminal nerves. Thus, this study aimed to investigate the potential link between the right and left trigeminal ganglia, considering their anatomical proximity and the possible neural interactions between these structures. The fluorogold (FG) neuronal tracer was localized in the neurons of both the right and left trigeminal ganglia (TG) after unilateral injection into the whisker pad. In contrast, there was a substantial decrease in FG localization in the contralateral ganglion following colchicine (CCh) administration or after midline incision surgery. Moreover, FG injected directly into the trigeminal ganglion was exclusively localized on the ipsilateral side. Additionally, systemic administration of FG revealed its localization not only within the bilateral TG neurons but also in the bilateral dorsal root ganglia (DRG) neurons, mirroring the findings following whisker pad injection. These findings suggest that bilateral transport of fluorogold occurs at the peripheral level, possibly through its diffusion from the injection site to the contralateral whisker pad and/or nerve axonal crossover through the midline. Further studies are required to clarify the specific peripheral pathways and explore the bilateral nerve connections.

en: Neuroscience Letters

ja: Neuroscience Letters


en: Essence of diagnosis and management of sleep bruxism

en: Journal of Oral Health and Biosciences

ja: Journal of Oral Health and Biosciences


en: Identification of microRNA signatures in peripheral blood of young women as potential biomarkers for metal allergy

en: MicroRNA (miRNA) is a short (19-24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact dermatitis, female participants with a positive metal allergy diagnosis ( = 3) were enrolled along with additional female participants with no medical history of metal allergy ( = 3). A patch test was performed on each participant. Peripheral blood was collected from all the participants before the patch test and at days 3 and 7 after starting the patch test. After total RNA was obtained from peripheral blood leukocytes and cDNA was generated, microarray analysis was performed to analyze the large-scale circulating miRNA profile. Real-time polymerase chain reaction (RT-PCR) was then used to clarify the overall target miRNA expression. Downregulation of hsa-let-7d-5p, hsa-miR-24-3p, hsa-miR-23b-3p, hsa-miR-26b-5p, and hsa-miR-150-5p was found on day 7. Certain miRNAs were confirmed using RT-PCR. These peripheral blood miRNAs could be diagnostic biomarkers for metal allergies.

en: Biomedicines

ja: Biomedicines


en: Analgesic effect of tranilast in an animal model of neuropathic pain and its role in the regulation of tetrahydrobiopterin synthesis

en: International Journal of Molecular Sciences


en: Peripherally Administered Botulinum Toxin Type A Localizes Bilaterally in Trigeminal Ganglia of Animal Model

en: Toxins

ja: Toxins


en: A case of fixed prosthodontic treatment through tooth preservation with occlusal traumatic symptoms

ja: 咬合性外傷による重度動揺歯に対して歯の保存を行い固定性補綴装置にて機能回復を行った症例

en:
Patient: A 64-year-old female patient complained of masticatory dysfunction caused by mobility of a maxillary right fixed bridge. The maxillary right canine with occlusal traumatic symptoms had been diagnosed by her previous dentist as indicated for extraction, but it was fixed with a provisional bridge to determine whether the tooth could recover. After this traumatic tooth was allowed to recover, the fabricated fixed bridge was finally attached after a follow-up observation by temporary cementation.

Discussion: There are no clear criteria for tooth preservation of occlusal traumatic teeth depending on bone resorption or tooth mobility. Therefore, it is important to plan a step-by-step prosthodontic treatment in which the availability of abutment teeth is determined after the acute traumatic symptoms have resolved.

Conclusion: A careful and step-by-step prosthodontic treatment for an occlusal traumatic tooth resulted in tooth preservation, restoration of masticatory function, and improvement of patient satisfaction.

ja:
症例の概要:患者は64歳女性．上顎右側ブリッジの動揺による咀嚼困難を主訴に来院した．咬合性外傷症状が認められた上顎右側犬歯に対して，暫間補綴装置による固定と咬合力の分散にて症状改善を図り，製作した固定性補綴装置を仮着して経過観察を行った後に装着した．

考察:咬合性外傷を有する歯の保存可否を判断するためには，適切な原因除去と急性症状の消退が得られたのちに，支台歯として利用が可能かを見極める段階的な補綴治療計画が重要であると考えられた．

結論:他院にて抜歯適応と診断された歯の保存可否を適切に判断した後に，固定性補綴装置を装着することにより，支台歯への利用と咀嚼機能の回復，患者満足度の改善に貢献できた．

en: Annals of Japan Prosthodontic Society

ja: 日本補綴歯科学会誌


en: Dentin-pulp complex tissue regeneration via three-dimensional cell sheet layering

en: The dentin-pulp complex is a unique structure in teeth that contains both hard and soft tissues. Generally, deep caries and trauma cause damage to the dentin-pulp complex, and if left untreated, this damage will progress to irreversible pulpitis. The aim of this study was to fabricate a layered cell sheet composed of rat dental pulp (DP) cells and odontogenic differentiation of pulp (OD) cells and to investigate the ability to regenerate the dentin-pulp complex in a scaffold tooth. We fabricated two single cell sheets composed of DP cells (DP cell sheet) or OD cells (OD cell sheet) and a layered cell sheet made by layering both cells. The characteristics of the fabricated cell sheets were analyzed using light microscopy, scanning electron microscope (SEM), hematoxylin-eosin (HE) staining, and immunohistochemistry (IHC). Furthermore, the cell sheets were transplanted into the subrenal capsule of immunocompromised mice for 8 weeks. After this, the regenerative capacity to form dentin-like tissue was evaluated using micro-computed tomography (micro-CT), HE staining, and IHC. The findings of SEM and IHC confirmed that layered cell sheets fabricated by stacking OD cells and DP cells maintained their cytological characteristics. Micro-CT of layered cell sheet transplants revealed a mineralized capping of the access cavity in the crown area, similar to that of natural dentin. In contrast, the OD cell sheet group demonstrated the formation of irregular fragments of mineralized tissue in the pulp cavity, and the DP cell sheet did not develop any hard tissue. Moreover, bone volume/tissue volume (BV/TV) showed a significant increase in hard tissue formation in the layered cell sheet group compared with that in the single cell sheet group ( < 0.05). HE staining also showed a combination of soft and hard tissue formation in the layered cell sheet group. Furthermore, IHC confirmed that the dentin-like tissue generated from the layered cell sheet expressed characteristic markers of dentin but not bone equivalent to that of a natural tooth. In conclusion, this study demonstrates the feasibility of regenerating dentin-pulp complex using a bioengineered tissue designed to simulate the anatomical structure. Impact statement The dentin-pulp complex can be destroyed by deep caries and trauma, which may cause pulpitis and progress to irreversible pulpitis, apical periodontitis, and even tooth loss. Current treatments cannot maintain pulp health, and teeth can become brittle. We developed a three-dimensional (3D) layered cell sheet using dental pulp cells and odontogenic differentiation of pulp cells for dentin-pulp complex regeneration. Our layered cell sheet enables the regeneration of an organized 3D dentin-pulp-like structure comparable with that of natural teeth. This layered cell sheet technology may contribute to dentin-pulp complex regeneration and provide a novel method for complex tissue engineering.

en: Tissue Engineering. Part C, Methods

ja: Tissue Engineering. Part C, Methods


en: Longitudinal comparison between flipped classroom and team-based learning in a prosthodontic class

en:
Active learning is a concept that allows students to study and learn actively by themselves to get knowledge. There are several methods of active learning, including flipped classroom (FC) and team-based learning (TBL). In FC, students are required to study before classes. In TBL, students also study before class, take individual readiness assurance test (iRAT) and team readiness assurance test (tRAT), then discuss group assignment projects (GAPs) during class. The purpose of this study was to compare the effectiveness between FC and TBL.
 The effectiveness of FC and TBL was assessed from the results of the term-end examinations, questionnaires and practice examinations. To check the difficulty of the term-end examinations, control dentists took the same examinations and we calculated the equating score with item response theory.
 Statistical analysis showed that the correct answer rate in term-end examinations was significantly different in comparison with the time of the trial, and for the participants (students and dentists). The term-end examination score of FC and TBL did not show a statistical difference. The student questionnaire showed that TBL had higher scores than FC on various factors such as student positive attitude, preparation, ingenuity of teacher and achieving the class goals. The crown & bridge score from the practice examination of 6th-year students who had FC + TBL were constantly higher than the Japanese national average score. The identification index of FC and TBL did not show the statistical difference and there was no statistical difference on item response theory between FC and TBL.


en: Risk factors for tooth loss in patients with 25 remaining teeth undergoing mid-long-term

en: International Journal of Environmental Research and Public Health


en: Mechanical homeostasis of liver sinusoid is involved in the initiation and termination of liver regeneration

en: Organogenesis and regeneration are fundamental for developmental progress and are associated with morphogenesis, size control and functional properties for whole-body homeostasis. The liver plays an essential role in maintaining homeostasis of the entire body through various functions, including metabolic functions, detoxification, and production of bile, via the three-dimensional spatial arrangement of hepatic lobules and has high regenerative capacity. The regeneration occurs as hypertrophy, which strictly controls the size and lobule structure. In this study, we established a three-dimensional sinusoidal network analysis method and determined valuable parameters after partial hepatectomy by comparison to the static phase of the liver. We found that mechanical homeostasis, which is crucial for organ morphogenesis and functions in various phenomena, plays essential roles in liver regeneration for both initiation and termination of liver regeneration, which is regulated by cytokine networks. Mechanical homeostasis plays critical roles in the initiation and termination of organogenesis, tissue repair and organ regeneration in coordination with cytokine networks.

en: Communications Biology

ja: Communications Biology


en: インドネシアの歯学部における学部教育

ja: インドネシアの歯学部における学部教育

en: The Journal of Japan Association of Oral Rehabilitation

ja: 日本口腔リハビリテーション学会雑誌


en: Immediate Effect of Masticatory Muscle Activity with Transcutaneous Electrical Nerve Stimulation in Muscle Pain of Temporomandibular Disorders Patients

en: Transcutaneous electrical nerve stimulation (TENS) is a non-invasive treatment modality for acute and chronic pain. However, little information for muscle activity is available on the immediate effects of TENS in masticatory muscle pain related to temporomandibular disorders (TMDs). The present study aimed to evaluate the immediate effects of TENS treatment on TMD-related muscle pain. Thirty-six patients with TMD-related muscle pain and 39 healthy subjects served as TMD and control groups, respectively. For objective evaluations, maximum mouth opening, and maximum bite force were measured before and after TENS. The pain intensity was assessed according to a 100-mm visual analog scale (VAS). TENS was applied to painful muscles for 20 min with frequencies of 100-200 Hz. The treatment outcome was evaluated using Global Rating of Change (GRC) scales. In the TMD group, VAS values significantly decreased after TENS. Although there was significant increase in the maximum mouth opening after TENS for only TMD group, the maximum bite force of both groups was significantly greater after TENS. According to GRC scales, one patient with TMD-related muscle pain expressed negative feelings after TENS. Conclusively, TENS treatment might quickly relieve pain in masticatory muscles and improve masticatory functions in patients with TMD-related muscle pain.

en: Journal of Clinical Medicine

ja: Journal of Clinical Medicine


en: Fibronectin-induced ductal formation in salivary gland self-organization model

en: Recent advances in tissue regeneration approaches including 3D organoids, were based on various 3D organogenesis models. However, 3D models are generally technique-sensitive and time-consuming. Thus, we utilized an existing model of submandibular salivary gland (SMG) to modify a simple and highly reproducible in vitro 3D culture model of primary SMG cells self-organization into a well-developed cell spheroid inside Matrigel substrate. We used this model to observe the collective multicellular behavior during spheroid formation. Further, we applied various quantitative approaches including real-time live imaging and immune histochemical image analysis to dissect the cellular dynamics during tissue patterning. On a time-scale of hours, we observed marked size and shape transformations in the developed 3D spheroid which resulted in a spatially-controlled growth differential from the canter to the periphery of the formed aggregates. Moreover, we investigated the effect of fibronectin (FN) on SMG cells self-organization using our simplified culture model. Interestingly, we discovered a novel role of FN in inducing duct-like elongation during initial stages of SMG bud formation. This in vitro model provides an excellent tool for analyzing the intercellular dynamics during early SMG tissue development as well as revealing a novel role of FN in SMG ductal expansion.

en: Developmental Dynamics

ja: Developmental Dynamics


en: Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model.

en: Whole-organ regeneration has great potential for the replacement of dysfunctional organs through the reconstruction of a fully functional bioengineered organ using three-dimensional cell manipulation in vitro. Recently, many basic studies of whole-tooth replacement using three-dimensional cell manipulation have been conducted in a mouse model. Further evidence of the practical application to human medicine is required to demonstrate tooth restoration by reconstructing bioengineered tooth germ using a postnatal large-animal model. Herein, we demonstrate functional tooth restoration through the autologous transplantation of bioengineered tooth germ in a postnatal canine model. The bioengineered tooth, which was reconstructed using permanent tooth germ cells, erupted into the jawbone after autologous transplantation and achieved physiological function equivalent to that of a natural tooth. This study represents a substantial advancement in whole-organ replacement therapy through the transplantation of bioengineered organ germ as a practical model for future clinical regenerative medicine.

en: Scientific Reports

ja: Scientific Reports


en: Challenges for stem cell-based "regenerative prosthodontics".

en: Journal of Prosthodontic Research