type: misc

label: url

en: Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering

en: Traditionally, generation of new plants with improved or desirable features has relied on laborious and time-consuming breeding techniques. Genome-editing technologies have led to a new era of genome engineering, enabling an effective, precise, and rapid engineering of the plant genomes. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) has emerged as a new genome-editing tool, extensively applied in various organisms, including plants. The use of CRISPR/Cas9 allows generating transgene-free genome-edited plants ("null segregants") in a short period of time. In this review, we provide a critical overview of the recent advances in CRISPR/Cas9 derived technologies for inducing mutations at target sites in the genome and controlling the expression of target genes. We highlight the major breakthroughs in applying CRISPR/Cas9 to plant engineering, and challenges toward the production of null segregants. We also provide an update on the efforts of engineering Cas9 proteins, newly discovered Cas9 variants, and novel CRISPR/Cas systems for use in plants. The application of CRISPR/Cas9 and related technologies in plant engineering will not only facilitate molecular breeding of crop plants but also accelerate progress in basic research.

en: BMC Plant Biology

ja: BMC Plant Biology


en: ヒトと植物細胞の細胞融合:16億年の時を超えた再会

ja: ヒトと植物細胞の細胞融合:16億年の時を超えた再会

en: 現代化学

ja: 現代化学


en: 不毛な大地で油を作る植物たち

ja: 不毛な大地で油を作る植物たち

en: 生物工学会誌

ja: 生物工学会誌