=== Generating (published_papers) ===
=== Generating (education) ===
=== Generating (research_experience) ===
=== Generating (misc) ===
=== Generating (research_projects) ===
=== Generating (books_etc) ===
=== Generating (industrial_property_rights) ===
=== Generating (awards) ===
=== Generating (presentations) ===
==== begin registerFile(/WWW/pub2/data/ERD/person/334009/researchmap/published_papers.jsonl) ====
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line:2, {"insert":{"user_id":"6000020075","type":"published_papers","id":"32792851"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/116611","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=376262","label":"url"}],"paper_title":{"en":"Genome editing in mammalian cells using the CRISPR type I-D nuclease","ja":"Genome editing in mammalian cells using the CRISPR type I-D nuclease"},"authors":{"en":[{"name":"Osakabe Keishi"},{"name":"Wada Naoki"},{"name":"Murakami Emi"},{"name":"Miyashita Naoyuki"},{"name":"Osakabe Yuriko"}],"ja":[{"name":"刑部 敬史"},{"name":"和田 直樹"},{"name":"村上 愛美"},{"name":"宮下 尚之"},{"name":"刑部 祐里子"}]},"publication_date":"2021-07-02","publication_name":{"en":"Nucleic Acids Research","ja":"Nucleic Acids Research"},"languages":["eng"],"referee":true,"identifiers":{"doi":["10.1093/nar/gkab348"],"issn":["1362-4962"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
line:3, {"insert":{"user_id":"6000020075","type":"published_papers","id":"32792852"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/116539","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=376263","label":"url"}],"paper_title":{"en":"Epigenetic Distribution of Recombinant Plant Chromosome Fragments in a HumanArabidopsis Hybrid Cell Line","ja":"Epigenetic Distribution of Recombinant Plant Chromosome Fragments in a HumanArabidopsis Hybrid Cell Line"},"authors":{"en":[{"name":"Liaw YengMun"},{"name":"Liu Yikun"},{"name":"Teo CheeHow"},{"name":"Cápal Petr"},{"name":"Wada Naoki"},{"name":"Fukui Kiichi"},{"name":"Doležel Jaroslav"},{"name":"Ohmido Nobuko."}],"ja":[{"name":"Liaw YengMun"},{"name":"Liu Yikun"},{"name":"Teo CheeHow"},{"name":"Cápal Petr"},{"name":"和田 直樹"},{"name":"Fukui Kiichi"},{"name":"Doležel Jaroslav"},{"name":"Ohmido Nobuko."}]},"publication_date":"2021-05-21","publication_name":{"en":"International Journal of Molecular Sciences","ja":"International Journal of Molecular Sciences"},"volume":"Vol.22","number":"No.11","starting_page":"5426","ending_page":"5426","languages":["eng"],"referee":true,"identifiers":{"doi":["10.3390/ijms22115426"],"issn":["1422-0067"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
line:4, {"insert":{"user_id":"6000020075","type":"published_papers","id":"32792853"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/116632","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=376265","label":"url"}],"paper_title":{"en":"Molecular organization of recombinant human-Arabidopsis chromosomes in hybrid cell lines","ja":"Molecular organization of recombinant human-Arabidopsis chromosomes in hybrid cell lines"},"authors":{"en":[{"name":"Liu Yikun"},{"name":"Liaw Mun Yeng"},{"name":"Teo How Chee"},{"name":"Cápal Petr"},{"name":"Wada Naoki"},{"name":"Fukui Kiichi"},{"name":"Doležel Jaroslav"},{"name":"Ohmido Nobuko"}],"ja":[{"name":"Liu Yikun"},{"name":"Liaw Mun Yeng"},{"name":"Teo How Chee"},{"name":"Cápal Petr"},{"name":"和田 直樹"},{"name":"Fukui Kiichi"},{"name":"Doležel Jaroslav"},{"name":"Ohmido Nobuko"}]},"publication_date":"2021-03-30","publication_name":{"en":"Scientific Reports","ja":"Scientific Reports"},"languages":["eng"],"referee":true,"identifiers":{"doi":["10.1038/s41598-021-86130-4"],"issn":["2045-2322"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
line:5, {"insert":{"user_id":"6000020075","type":"published_papers","id":"32792854"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/116525","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=376264","label":"url"}],"paper_title":{"en":"Effects of the sliaa9 Mutation on Shoot Elongation Growth of Tomato Cultivars","ja":"Effects of the sliaa9 Mutation on Shoot Elongation Growth of Tomato Cultivars"},"authors":{"en":[{"name":"Abe-Hara Chihiro"},{"name":"Yamada Kohji"},{"name":"Wada Naoki"},{"name":"Ueta Risa"},{"name":"Hashimoto Ryosuke"},{"name":"Osakabe Keishi"},{"name":"Osakabe Yuriko"}],"ja":[{"name":"Abe-Hara Chihiro"},{"name":"山田 晃嗣"},{"name":"和田 直樹"},{"name":"Ueta Risa"},{"name":"Hashimoto Ryosuke"},{"name":"刑部 敬史"},{"name":"Osakabe Yuriko"}]},"publication_date":"2021-03-20","publication_name":{"en":"Frontiers in Plant Science","ja":"Frontiers in Plant Science"},"languages":["eng"],"referee":true,"identifiers":{"doi":["10.3389/fpls.2021.627832"],"issn":["1664-462X"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
line:6, {"insert":{"user_id":"6000020075","type":"published_papers","id":"32792855"},"force":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/115886","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=372338","label":"url"}],"paper_title":{"en":"Genome editing in plants using CRISPR type I-D nuclease.","ja":"Genome editing in plants using CRISPR type I-D nuclease."},"authors":{"en":[{"name":"Osakabe Keishi"},{"name":"Wada Naoki"},{"name":"Tomoko Miyaji"},{"name":"Emi Murakami"},{"name":"Kazuya Marui"},{"name":"Risa Ueta"},{"name":"Ryosuke Hashimoto"},{"name":"Chihiro Abe-Hara"},{"name":"Bihe Kong"},{"name":"Kentaro Yano"},{"name":"Osakabe Yuriko"}],"ja":[{"name":"刑部 敬史"},{"name":"和田 直樹"},{"name":"Tomoko Miyaji"},{"name":"Emi Murakami"},{"name":"Kazuya Marui"},{"name":"上田 梨紗"},{"name":"橋本 諒典"},{"name":"Chihiro Abe-Hara"},{"name":"Bihe Kong"},{"name":"Kentaro Yano"},{"name":"刑部 祐里子"}]},"publication_date":"2020-11-06","publication_name":{"en":"Communications Biology","ja":"Communications Biology"},"volume":"Vol.3","starting_page":"648","ending_page":"648","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1038/s42003-020-01366-6"],"issn":["2399-3642"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
line:7, {"insert":{"user_id":"6000020075","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=337333","label":"url"}],"paper_title":{"en":"Association Study of Seed-Yield Related Traits for Jatropha curcas L. in Mexico","ja":"Association Study of Seed-Yield Related Traits for Jatropha curcas L. in Mexico"},"authors":{"en":[{"name":"Li Haiyan"},{"name":"Tsuchimoto Suguru"},{"name":"Harada Kyuya"},{"name":"Yamasaki Masanori"},{"name":"Sakai HIroe"},{"name":"Wada Naoki"},{"name":"Alipour Arefeh"},{"name":"Sasai Tomohiro"},{"name":"Tunekawa Atsushi"},{"name":"Tsujimoto Hisashi"},{"name":"Ando Takayuki"},{"name":"Tomemori Hisashi"},{"name":"Sato Shusei"},{"name":"Hirakawa HIdeaki"},{"name":"Pecina-Quintero Victor"},{"name":"Zamarripa Alfredo"},{"name":"Fukui Kiichi"}],"ja":[{"name":"Li Haiyan"},{"name":"Tsuchimoto Suguru"},{"name":"Harada Kyuya"},{"name":"Yamasaki Masanori"},{"name":"Sakai HIroe"},{"name":"和田 直樹"},{"name":"Alipour Arefeh"},{"name":"Sasai Tomohiro"},{"name":"Tunekawa Atsushi"},{"name":"Tsujimoto Hisashi"},{"name":"Ando Takayuki"},{"name":"Tomemori Hisashi"},{"name":"Sato Shusei"},{"name":"Hirakawa HIdeaki"},{"name":"Pecina-Quintero Victor"},{"name":"Zamarripa Alfredo"},{"name":"Fukui Kiichi"}]},"publication_date":"2018","publication_name":{"en":"Tropical Agriculture and Development","ja":"Tropical Agriculture and Development"},"volume":"Vol.62","number":"No.2","starting_page":"68","ending_page":"77","languages":["eng"],"referee":true,"identifiers":{"doi":["10.11248/jsta.62.68"],"issn":["1882-8450"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
line:8, {"insert":{"user_id":"6000020075","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://repo.lib.tokushima-u.ac.jp/ja/114486","label":"url"},{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/28936216","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=337334","label":"url"}],"paper_title":{"en":"Genetic Tracing of Jatropha curcas L. from Its Mesoamerican Origin to the World.","ja":"Genetic Tracing of Jatropha curcas L. from Its Mesoamerican Origin to the World."},"authors":{"en":[{"name":"Li Haiyan"},{"name":"Tsuchimoto Suguru"},{"name":"Harada Kyuya"},{"name":"Yamasaki Masanori"},{"name":"Sakai Hiroe"},{"name":"Wada Naoki"},{"name":"Alipour Atefeh"},{"name":"Sasai Tomohiro"},{"name":"Tsunekawa Atsushi"},{"name":"Tsujimoto Hisashi"},{"name":"Ando Takayuki"},{"name":"Tomemori Hisashi"},{"name":"Sato Shusei"},{"name":"Hirakawa Hideki"},{"name":"Quintero Victor P"},{"name":"Zamarripa Alfredo"},{"name":"Santos Primitivo"},{"name":"Hegazy Adel"},{"name":"Ali Abdalla M"},{"name":"Fukui Kiichi"}],"ja":[{"name":"Li Haiyan"},{"name":"Tsuchimoto Suguru"},{"name":"Harada Kyuya"},{"name":"Yamasaki Masanori"},{"name":"Sakai Hiroe"},{"name":"和田 直樹"},{"name":"Alipour Atefeh"},{"name":"Sasai Tomohiro"},{"name":"Tsunekawa Atsushi"},{"name":"Tsujimoto Hisashi"},{"name":"Ando Takayuki"},{"name":"Tomemori Hisashi"},{"name":"Sato Shusei"},{"name":"Hirakawa Hideki"},{"name":"Quintero Victor P"},{"name":"Zamarripa Alfredo"},{"name":"Santos Primitivo"},{"name":"Hegazy Adel"},{"name":"Ali Abdalla M"},{"name":"Fukui Kiichi"}]},"description":{"en":"L. (Jatropha), a shrub species of the family Euphorbiaceae, has been recognized as a promising biofuel plant for reducing greenhouse gas emissions. However, recent attempts at commercial cultivation in Africa and Asia have failed because of low productivity. It is important to elucidate genetic diversity and relationship in worldwide Jatropha genetic resources for breeding of better commercial cultivars. Here, genetic diversity was analyzed by using 246 accessions from Mesoamerica, Africa and Asia, based on 59 simple sequence repeat markers and eight retrotransposon-based insertion polymorphism markers. We found that central Chiapas of Mexico possesses the most diverse genetic resources, and the Chiapas Central Depression could be the center of origin. We identified three genetic groups in Mesoamerica, whose distribution revealed a distinct geographic cline. One of them consists mainly of accessions from central Chiapas. This suggests that it represents the original genetic group. We found two Veracruz accessions in another group, whose ancestors might be shipped from Port of Veracruz to the Old World, to be the source of all African and Asian Jatropha. Our results suggest the human selection that caused low productivity in Africa and Asia, and also breeding strategies to improve African and Asian Jatropha. Cultivars improved in the productivity will contribute to expand mass commercial cultivation of Jatropha in Africa and Asia to increase biofuel production, and finally will support in the battle against the climate change.","ja":"L. (Jatropha), a shrub species of the family Euphorbiaceae, has been recognized as a promising biofuel plant for reducing greenhouse gas emissions. However, recent attempts at commercial cultivation in Africa and Asia have failed because of low productivity. It is important to elucidate genetic diversity and relationship in worldwide Jatropha genetic resources for breeding of better commercial cultivars. Here, genetic diversity was analyzed by using 246 accessions from Mesoamerica, Africa and Asia, based on 59 simple sequence repeat markers and eight retrotransposon-based insertion polymorphism markers. We found that central Chiapas of Mexico possesses the most diverse genetic resources, and the Chiapas Central Depression could be the center of origin. We identified three genetic groups in Mesoamerica, whose distribution revealed a distinct geographic cline. One of them consists mainly of accessions from central Chiapas. This suggests that it represents the original genetic group. We found two Veracruz accessions in another group, whose ancestors might be shipped from Port of Veracruz to the Old World, to be the source of all African and Asian Jatropha. Our results suggest the human selection that caused low productivity in Africa and Asia, and also breeding strategies to improve African and Asian Jatropha. Cultivars improved in the productivity will contribute to expand mass commercial cultivation of Jatropha in Africa and Asia to increase biofuel production, and finally will support in the battle against the climate change."},"publication_date":"2017-09-07","publication_name":{"en":"Frontiers in Plant Science","ja":"Frontiers in Plant Science"},"volume":"Vol.8","number":"No.1539","languages":["eng"],"referee":true,"identifiers":{"doi":["10.3389/fpls.2017.01539"],"issn":["1664-462X"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
line:9, {"insert":{"user_id":"6000020075","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=337339","label":"url"}],"paper_title":{"en":"Agrobacterium-Mediated Transformation of Jojoba [Simmondsia chinensis (Link) Schneider]","ja":"Agrobacterium-Mediated Transformation of Jojoba [Simmondsia chinensis (Link) Schneider]"},"authors":{"en":[{"name":"Mohammed A Ismail"},{"name":"Tsuchimoto Suguru"},{"name":"Sakai Hiroe"},{"name":"Wada Naoki"},{"name":"Fukuui Kiichi"}],"ja":[{"name":"Mohammed A Ismail"},{"name":"Tsuchimoto Suguru"},{"name":"Sakai Hiroe"},{"name":"和田 直樹"},{"name":"Fukuui Kiichi"}]},"publication_date":"2017","publication_name":{"en":"Tropical Agriculture and Development","ja":"Tropical Agriculture and Development"},"volume":"Vol.61","number":"No.1","starting_page":"23","ending_page":"31","languages":["eng"],"referee":true,"identifiers":{"doi":["10.11248/jsta.61.23"],"issn":["1882-8469"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
line:10, {"insert":{"user_id":"6000020075","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=337338","label":"url"}],"paper_title":{"en":"High-frequency Shoots Regeneration of an Oil Crop, Simmondsia chinensis (Link) Schneider Using Axillary Buds.","ja":"High-frequency Shoots Regeneration of an Oil Crop, Simmondsia chinensis (Link) Schneider Using Axillary Buds."},"authors":{"en":[{"name":"Mohammed A Ismail"},{"name":"Tsuchimoto Suguru"},{"name":"Sakai Hiroe"},{"name":"Wada Naoki"},{"name":"Fukui Kiichi"}],"ja":[{"name":"Mohammed A Ismail"},{"name":"Tsuchimoto Suguru"},{"name":"Sakai Hiroe"},{"name":"和田 直樹"},{"name":"Fukui Kiichi"}]},"publication_date":"2017","publication_name":{"en":"Tropical Agriculture and Development","ja":"Tropical Agriculture and Development"},"volume":"Vol.61","number":"No.1","starting_page":"15","ending_page":"22","languages":["eng"],"referee":true,"identifiers":{"doi":["10.11248/jsta.61.15"],"issn":["1882-8469"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
line:11, {"insert":{"user_id":"6000020075","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/27696824","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=337335","label":"url"}],"paper_title":{"en":"Maintenance and Function of a Plant Chromosome in Human Cells.","ja":"Maintenance and Function of a Plant Chromosome in Human Cells."},"authors":{"en":[{"name":"Wada Naoki"},{"name":"Kazuki Yasuhiro"},{"name":"Kazuki Kanako"},{"name":"Inoue Toshiaki"},{"name":"Fukui Kiichi"},{"name":"Oshimura Mitsuo"}],"ja":[{"name":"和田 直樹"},{"name":"Kazuki Yasuhiro"},{"name":"Kazuki Kanako"},{"name":"Inoue Toshiaki"},{"name":"Fukui Kiichi"},{"name":"Oshimura Mitsuo"}]},"description":{"en":"Replication, segregation, gene expression, and inheritance are essential features of all eukaryotic chromosomes. To delineate the extent of conservation of chromosome functions between humans and plants during evolutionary history, we have generated the first human cell line containing an Arabidopsis chromosome. The Arabidopsis chromosome was mitotically stable in hybrid cells following cell division, and initially existed as a translocated chromosome. During culture, the translocated chromosomes then converted to two types of independent plant chromosomes without human DNA sequences, with reproducibility. One pair of localization signals of CENP-A, a marker of functional centromeres was detected in the Arabidopsis genomic region in independent plant chromosomes. These results suggest that the chromosome maintenance system was conserved between human and plants. Furthermore, the expression of plant endogenous genes was observed in the hybrid cells, implicating that the plant chromosomal region existed as euchromatin in a human cell background and the gene expression system is conserved between two organisms. The present study suggests that the essential chromosome functions are conserved between evolutionarily distinct organisms such as humans and plants. Systematic analyses of hybrid cells may lead to the production of a shuttle vector between animal and plant, and a platform for the genome writing.","ja":"Replication, segregation, gene expression, and inheritance are essential features of all eukaryotic chromosomes. To delineate the extent of conservation of chromosome functions between humans and plants during evolutionary history, we have generated the first human cell line containing an Arabidopsis chromosome. The Arabidopsis chromosome was mitotically stable in hybrid cells following cell division, and initially existed as a translocated chromosome. During culture, the translocated chromosomes then converted to two types of independent plant chromosomes without human DNA sequences, with reproducibility. One pair of localization signals of CENP-A, a marker of functional centromeres was detected in the Arabidopsis genomic region in independent plant chromosomes. These results suggest that the chromosome maintenance system was conserved between human and plants. Furthermore, the expression of plant endogenous genes was observed in the hybrid cells, implicating that the plant chromosomal region existed as euchromatin in a human cell background and the gene expression system is conserved between two organisms. The present study suggests that the essential chromosome functions are conserved between evolutionarily distinct organisms such as humans and plants. Systematic analyses of hybrid cells may lead to the production of a shuttle vector between animal and plant, and a platform for the genome writing."},"publication_date":"2016-10-17","publication_name":{"en":"ACS Synthetic Biology","ja":"ACS Synthetic Biology"},"volume":"Vol.6","number":"No.2","starting_page":"301","ending_page":"310","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1021/acssynbio.6b00180"],"issn":["2161-5063"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
line:12, {"insert":{"user_id":"6000020075","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=337340","label":"url"}],"paper_title":{"en":"Localization of transgene-derived friabilins in rice endosperm cells","ja":"Localization of transgene-derived friabilins in rice endosperm cells"},"authors":{"en":[{"name":"Fujiwara Misa"},{"name":"Suzuki Go"},{"name":"Kudo Daiki"},{"name":"Oba Haruna"},{"name":"Wada Yukio"},{"name":"Wada Hideo"},{"name":"Wada Naoki"},{"name":"Rahman Sadequr"},{"name":"Fukui Kiichi"},{"name":"Mukai Yasuhiko"}],"ja":[{"name":"Fujiwara Misa"},{"name":"Suzuki Go"},{"name":"Kudo Daiki"},{"name":"Oba Haruna"},{"name":"Wada Yukio"},{"name":"Wada Hideo"},{"name":"和田 直樹"},{"name":"Rahman Sadequr"},{"name":"Fukui Kiichi"},{"name":"Mukai Yasuhiko"}]},"publication_date":"2014","publication_name":{"en":"Plant Biotechnology","ja":"Plant Biotechnology"},"volume":"Vol.31","number":"No.1","starting_page":"67","ending_page":"70","languages":["eng"],"referee":true,"identifiers":{"doi":["10.5511/plantbiotechnology.13.1028a"],"issn":["1347-6114"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
line:13, {"insert":{"user_id":"6000020075","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=337341","label":"url"}],"paper_title":{"en":"Development of transgenic plants in jatropha with drought tolerance","ja":"Development of transgenic plants in jatropha with drought tolerance"},"authors":{"en":[{"name":"Tsuchimoto Suguru"},{"name":"Cartagena A Joyce"},{"name":"Naruemon Khemkladngoen"},{"name":"Suthitar Singkaravanit"},{"name":"Kohinata Tsutomu"},{"name":"Wada Naoki"},{"name":"Sakai HIroe"},{"name":"Shibata Daisuke"},{"name":"Fukui Kiichi"}],"ja":[{"name":"Tsuchimoto Suguru"},{"name":"Cartagena A Joyce"},{"name":"Naruemon Khemkladngoen"},{"name":"Suthitar Singkaravanit"},{"name":"Kohinata Tsutomu"},{"name":"和田 直樹"},{"name":"Sakai HIroe"},{"name":"Shibata Daisuke"},{"name":"Fukui Kiichi"}]},"publication_date":"2012","publication_name":{"en":"Plant Biotechnology","ja":"Plant Biotechnology"},"volume":"Vol.29","number":"No.2","starting_page":"137","ending_page":"143","languages":["eng"],"referee":true,"identifiers":{"doi":["10.5511/plantbiotechnology.12.0406d"],"issn":["1347-6114"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
line:14, {"insert":{"user_id":"6000020075","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/21149391","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=337342","label":"url"}],"paper_title":{"en":"Sequence analysis of the genome of an oil-bearing tree, Jatropha curcas L.","ja":"Sequence analysis of the genome of an oil-bearing tree, Jatropha curcas L."},"authors":{"en":[{"name":"Sato Shusei"},{"name":"Hirakawa Hideki"},{"name":"Isobe Sachiko"},{"name":"Fukai Eigo"},{"name":"Watanabe Akiko"},{"name":"Kato Midori"},{"name":"Kawashima Kumiko"},{"name":"Minami Chiharu"},{"name":"Muraki Akiko"},{"name":"Nakazaki Naomi"},{"name":"Takahashi Chika"},{"name":"Nakayama Shinobu"},{"name":"Kishida Yoshie"},{"name":"Kohara Mitsuyo"},{"name":"Yamada Manabu"},{"name":"Tsuruoka Hisano"},{"name":"Sasamoto Shigemi"},{"name":"Tabata Satoshi"},{"name":"Aizu Tomoyuki"},{"name":"Toyoda Atsushi"},{"name":"Shin-i Tadasu"},{"name":"Minakuchi Yohei"},{"name":"Kohara Yuji"},{"name":"Fujiyama Asao"},{"name":"Tsuchimoto Suguru"},{"name":"Kajiyama Shin'ichiro"},{"name":"Makigano Eri"},{"name":"Ohmido Nobuko"},{"name":"Shibagaki Nakako"},{"name":"Cartagena Joyce A"},{"name":"Wada Naoki"},{"name":"Kohinata Tsutomu"},{"name":"Atefeh Alipour"},{"name":"Yuasa Shota"},{"name":"Matsunaga Sachihiro"},{"name":"Fukui Kiichi"}],"ja":[{"name":"Sato Shusei"},{"name":"Hirakawa Hideki"},{"name":"Isobe Sachiko"},{"name":"Fukai Eigo"},{"name":"Watanabe Akiko"},{"name":"Kato Midori"},{"name":"Kawashima Kumiko"},{"name":"Minami Chiharu"},{"name":"Muraki Akiko"},{"name":"Nakazaki Naomi"},{"name":"Takahashi Chika"},{"name":"Nakayama Shinobu"},{"name":"Kishida Yoshie"},{"name":"Kohara Mitsuyo"},{"name":"Yamada Manabu"},{"name":"Tsuruoka Hisano"},{"name":"Sasamoto Shigemi"},{"name":"Tabata Satoshi"},{"name":"Aizu Tomoyuki"},{"name":"Toyoda Atsushi"},{"name":"Shin-i Tadasu"},{"name":"Minakuchi Yohei"},{"name":"Kohara Yuji"},{"name":"Fujiyama Asao"},{"name":"Tsuchimoto Suguru"},{"name":"Kajiyama Shin'ichiro"},{"name":"Makigano Eri"},{"name":"Ohmido Nobuko"},{"name":"Shibagaki Nakako"},{"name":"Cartagena Joyce A"},{"name":"和田 直樹"},{"name":"Kohinata Tsutomu"},{"name":"Atefeh Alipour"},{"name":"Yuasa Shota"},{"name":"Matsunaga Sachihiro"},{"name":"Fukui Kiichi"}]},"description":{"en":"The whole genome of Jatropha curcas was sequenced, using a combination of the conventional Sanger method and new-generation multiplex sequencing methods. Total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. They accounted for ~95% of the gene-containing regions with the average G + C content was 34.3%. A total of 40 929 complete and partial structures of protein encoding genes have been deduced. Comparison with genes of other plant species indicated that 1529 (4%) of the putative protein-encoding genes are specific to the Euphorbiaceae family. A high degree of microsynteny was observed with the genome of castor bean and, to a lesser extent, with those of soybean and Arabidopsis thaliana. In parallel with genome sequencing, cDNAs derived from leaf and callus tissues were subjected to pyrosequencing, and a total of 21 225 unigene data have been generated. Polymorphism analysis using microsatellite markers developed from the genomic sequence data obtained was performed with 12 J. curcas lines collected from various parts of the world to estimate their genetic diversity. The genomic sequence and accompanying information presented here are expected to serve as valuable resources for the acceleration of fundamental and applied research with J. curcas, especially in the fields of environment-related research such as biofuel production. Further information on the genomic sequences and DNA markers is available at http://www.kazusa.or.jp/jatropha/.","ja":"The whole genome of Jatropha curcas was sequenced, using a combination of the conventional Sanger method and new-generation multiplex sequencing methods. Total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. They accounted for ~95% of the gene-containing regions with the average G + C content was 34.3%. A total of 40 929 complete and partial structures of protein encoding genes have been deduced. Comparison with genes of other plant species indicated that 1529 (4%) of the putative protein-encoding genes are specific to the Euphorbiaceae family. A high degree of microsynteny was observed with the genome of castor bean and, to a lesser extent, with those of soybean and Arabidopsis thaliana. In parallel with genome sequencing, cDNAs derived from leaf and callus tissues were subjected to pyrosequencing, and a total of 21 225 unigene data have been generated. Polymorphism analysis using microsatellite markers developed from the genomic sequence data obtained was performed with 12 J. curcas lines collected from various parts of the world to estimate their genetic diversity. The genomic sequence and accompanying information presented here are expected to serve as valuable resources for the acceleration of fundamental and applied research with J. curcas, especially in the fields of environment-related research such as biofuel production. Further information on the genomic sequences and DNA markers is available at http://www.kazusa.or.jp/jatropha/."},"publication_date":"2010-12-13","publication_name":{"en":"DNA Research","ja":"DNA Research"},"volume":"Vol.18","number":"No.1","starting_page":"65","ending_page":"76","languages":["eng"],"referee":true,"identifiers":{"doi":["10.1093/dnares/dsq030"],"issn":["1756-1663"]},"published_paper_type":"scientific_journal"},"priority":"input_data"}
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