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en: Differential Expression of Key Isoflavone Synthesis Genes in Soybean Sprouts under Two LED Treatments

en: Soybean seeds and sprouts contain isoflavones, including aglycones, glucosides, and malonylated glucosides, and their contents vary across soybean varieties. During germination and photomorphogenesis, blue light-emitting diode (LED) irradiation suppressed hypocotyl growth and enhanced the expression levels of chalcone synthase 7, isoflavone synthase (IFS), and isoflavone reductase (IFR). Conversely, red LED irradiation enhanced hypocotyl growth and decreased the expression levels of IFS and IFR. The contents of some glycosides or malonylated glycosides in sprouts and young green soybeans were significantly higher under blue LED irradiation than under red LED irradiation. These results imply that blue light more effectively increased the concentrations of some isoflavones during early germination and/or hydroponics cultivation.

en: ACS Agricultural Science & Technology

ja: ACS Agricultural Science & Technology


en: Analysis of myofiber composition in myostatin monoallelic mutant pigs

en: Journal of Livestock Science

ja: Journal of Livestock Science


en: Effects of Electroporation Timing and Cumulus Cell Attachment on In Vitro Development and Genome Editing of Porcine Embryos.

en: Pig genome editing using the oviductal nucleic acid delivery (GONAD) method with electroporation would allow the efficient obtention of genetically modified pigs. However, oocytes and zygotes at various stages after ovulation must be targeted, and cumulus cell attachment and mosaic mutations are major obstacles. Therefore, we investigated whether two parameters (electroporation timing and the cumulus cell attachment) influence the effectiveness of multiplex genome editing by electroporation in porcine oocytes or zygotes. Three gRNAs targeting either GGTA1, CMAH or B4GALNT2 were introduced individually into oocytes and zygotes with and without cumulus cells at three different time points, 0 h before in vitro fertilisation (IVF) and 5 h and 10 h after IVF initiation. The introduction of gRNAs into oocytes and zygotes did not significantly affect the rates of blastocyst formation and total mutation of the resulting blastocysts irrespective of cumulus cell attachment and electroporation timing. In conclusion, the electroporation timing and the cumulus cell attachment did not interfere with the efficient delivery of the CRISPR/Cas9 system to the oocytes/zygotes, indicating that porcine genome editing in the oviduct using GONAD method may be possible.

en: Reproduction in Domestic Animals = Zuchthygiene


en: Efficient gene editing of pig embryos by combining electroporation and lipofection.

en: Electroporation combined with lipofection using gRNAs with low mutation rates may improve gene editing efficiency in pig embryos. However, the effects may vary based on the timing of gene editing.

en: Veterinary World

ja: Veterinary World


en: Optimization of embryonic stage for aggregation to generate chimeric pigs using gene-edited blastomeres.

en: In Vitro Cellular & Developmental Biology. Animal


en: Blue LED light promotes indican accumulation and flowering in indigo plant, Polygonum tinctorium

en: Industrial Crops and Products

ja: Industrial Crops and Products