Tokushima University / Educator and Researcher Directory --- Ikuta, Takahisa

Personal Web Page

https://researchmap.jp/namadent (researchmap)

Field of Study

Subject of Study

Book / Paper

Book:

1.	高柴正悟, 池田淳史, Hiromichi Yumoto, Masami Ninomiya and Takahisa Ikuta : WORKGROUP 2: PERIODONTITIS, 急性歯周疾患(歯周膿瘍と壊死性歯周疾患)と歯内―歯周病変, Oct. 2020.

Academic Paper (Judged Full Paper):

1.	Rie Kido, Yuka Hiroshima, Jun-ichi Kido, Takahisa Ikuta, Eijiro Sakamoto, Yuji Inagaki, Koji Naruishi and Hiromichi Yumoto : Advanced glycation end-products increase lipocalin 2 expression in human oral epithelial cells., Journal of Periodontal Research, Vol.55, No.4, 539-550, 2020. (Tokushima University Institutional Repository) ● Metadata: 114681 (Link to Publication Site) ● Publication site (DOI): 10.1111/jre.12741 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32170733 ● Summary page in Scopus @ Elsevier: 2-s2.0-85081730917 (Tokushima University Institutional Repository: 114681, DOI: 10.1111/jre.12741, PubMed: 32170733, Elsevier: Scopus)
2.	Koji Naruishi, Eijiro Sakamoto, Takahisa Ikuta, Rie Kido, Jun-ichi Kido and Hiromichi Yumoto : Evaluation of educational effects of periodontology by lectures and phantom training in dental students, The Japanese Journal of Conservative Dentistry, Vol.63, No.1, 22-29, 2020. (Summary) <p> Purpose: In the undergraduate educational curriculum of dentistry, there are two main teaching/learning methods for dental students before clinical training: traditional lectures and phantom training. The purpose of this study was to explore effective educational systems for periodontology by examining the test results of both lectures and phantom training.</p><p> Methods: Both a written test and a practical test of periodontology were administered to students of dentistry. A total of 43 dental students of Tokushima University Dental School were recruited. Written tests were performed before and after the lectures on periodontology. A practical test of scaling and root planing was performed after phantom training. Associations between the results of the written test and practical test were analyzed statistically. P values of less than 0.05 were considered statistically significant.</p><p> Results: The results of the written tests after lectures were higher than those before lectures. A significant positive correlation was found between the results of the written test and practical test.</p><p> Conclusion: In the students' education on periodontology, the results of the practical test were correlated statistically to the results of the written test. These findings may contribute to establishing effective educational systems for periodontology in dentistry.</p> (Keyword) 歯学教育 / 歯周病学 / 基礎(模型)実習試験 / 筆記試験 (Link to Publication Site) ● Publication site (DOI): 10.11471/shikahozon.63.22 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390002184879112064 ● Search Scopus @ Elsevier (DOI): 10.11471/shikahozon.63.22 (DOI: 10.11471/shikahozon.63.22, CiNii: 1390002184879112064)
3.	Kohei Nonaka, Yukari Kajiura, Mika Bandou, Eijiro Sakamoto, Yuji Inagaki, JH Lew, Koji Naruishi, Takahisa Ikuta, Kaya Yoshida, Tesuo Kobayashi, Hiromasa Yoshie, Toshihiko Nagata and Jun-ichi Kido : Advanced glycation end-products increase IL-6 and ICAM-1 expression via RAGE, MAPK and NF-kB pathways in human gingival fibroblasts, Journal of Periodontal Research, Vol.53, No.3, 334-344, 2018. (Summary) Diabetes mellitus (DM) is a risk factor for periodontal diseases and may exacerbate the progression of the pathogenesis of periodontitis. Advanced glycation end-products (AGEs) cause DM complications relative to levels of glycemic control and larger amounts accumulate in the periodontal tissues of patients with periodontitis and DM. In the present study, we investigated the effects of AGEs on the expression of inflammation-related factors in human gingival fibroblasts (HGFs) to elucidate the impact of AGEs on DM-associated periodontitis. HGFs were cultured with or without AGEs. Cell viability was examined, and RNA and protein fractions were isolated from AGE-treated cells. The expression of interleukin (IL)-6, intercellular adhesion molecule-1 (ICAM-1), and the receptor for AGE (RAGE) was investigated using reverse transcription-polymerase chain reaction, quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, and reactive oxygen species activity was measured using a kit with 2',7'-dichlorofluorescin diacetate. Human monocytic cells (THP-1) labeled with a fluorescent reagent were co-cultured with HGFs treated with AGEs and IL-6 siRNA, and the adhesive activity of THP-1 cells to HGFs was assessed. The expression of IL-6 and ICAM-1 was examined when HGFs were pretreated with recombinant human IL-6, the siRNAs of RAGE and IL-6, and inhibitors of MAPK and NF-κB, and then cultured with and without AGEs. The phosphorylation of MAPK and NF-κB was assessed using western blotting. AGEs increased the mRNA and protein expressions of RAGE, IL-6, ICAM-1 and reactive oxygen species activity in HGFs, and promoted the adhesion of THP-1 cells to HGFs, but had no effect on cell viability until 72 hours. Recombinant human IL-6 increased ICAM-1 expression in HGFs, while the siRNAs of RAGE and IL-6 inhibited AGE-induced IL6 and ICAM1 mRNA expression, and IL-6 siRNA depressed AGE-induced THP-1 cell adhesion. AGEs increased the phosphorylation of p38 and ERK MAPKs, p65 NF-κB and IκBα, while inhibitors of p38, ERK MAPKs and NF-κB significantly decreased AGE-induced IL-6 and ICAM-1 expression. AGEs increase IL-6 and ICAM-1 expression via the RAGE, MAPK and NF-κB pathways in HGFs and may exacerbate the progression of the pathogenesis of periodontal diseases. (Keyword) Antigens, Neoplasm / Diabetes Complications / Fibroblasts / Gingiva / Glycation End Products, Advanced / Humans / Intercellular Adhesion Molecule-1 / Interleukin-6 / MAP Kinase Signaling System / Mitogen-Activated Protein Kinase Kinases / Mitogen-Activated Protein Kinases / NF-kappa B / Periodontitis / Phosphorylation / Reactive Oxygen Species / THP-1 Cells (Tokushima University Institutional Repository) ● Metadata: 113617 (Link to Publication Site) ● Publication site (DOI): 10.1111/jre.12518 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29193068 ● Summary page in Scopus @ Elsevier: 2-s2.0-85036593558 (Tokushima University Institutional Repository: 113617, DOI: 10.1111/jre.12518, PubMed: 29193068, Elsevier: Scopus)

Proceeding of International Conference:

1.	Makoto Funaki, Akiko Hata, Mitsuteru Yoshida, Naoki Miyamoto, Takahisa Ikuta, Yuta Uemura, MINATO AKIZUKI, Akira Tangoku, Hiromichi Yumoto, Hirohisa Ogawa and Takeshi Oya : Quiescence Abrogates Stress-Induced Senescence-Associated Secretion Phenotype in Adipose-Derived Stem Cells and May Enhance Their Therapeutic Effects on Diabetic Complications, Diabetes, Vol.71, No.Suppl_1, 305-OR, New Orleans, Jun. 2022.

Proceeding of Domestic Conference:

1.	Masami Ninomiya, Takahisa Ikuta, AKIZUKI Minato, Yuta Uemura and Hiromichi Yumoto : PCRを用いたTreponeme denticola, Tannerella forsythia迅速検出システムの有用性, 日本歯科保存学会 2023年度春季学術大会(第158回)プログラムおよび講演抄録集, 142, Jun. 2023. (Keyword) 歯周病原細菌 / Point of Care Testing / Porphyromonas gingivalis / Treponeme denticola / Tannerella forsythia
2.	Rie Kido, Yuka Hiroshima, Jun-ichi Kido, Kaya Yoshida, Takahisa Ikuta and Hiromichi Yumoto : Antimicrobial activity of artificially synthesized secretory leukocyte protease inhibitor and its encapsulation into liposomes, 第66回春季日本歯周病学会学術大会, May 2023.
3.	Makoto Funaki, Akiko Hata, Mitsuteru Yoshida, Naoki Miyamoto, Takahisa Ikuta, Yuta Uemura, Minato Akizuki, Akira Tangoku, Hiromichi Yumoto, Hirohisa Ogawa and Takeshi Oya : 休眠状態の脂肪組織由来間葉系幹細胞による細胞老化随伴分泌現象の解除と糖尿病性合併症治療への応用の可能性, Journal of the The Japan Diabetes Society, Vol.66, No.Suppl.1, S.246, May 2023.
4.	Yuji Inagaki, Mika Bandou, Takahisa Ikuta, Jun-ichi Kido and Hiromichi Yumoto : 侵襲性歯周炎(ステージIII, グレードC)に対して歯周基本治療と歯周外科治療を行い奏功した一症例, 第65回秋季日本歯周病学会学術大会(仙台), Sep. 2022.
5.	Makoto Funaki, Mitsuteru Yoshida, Naoki Miyamoto, Takahisa Ikuta, Yuta Uemura, MINATO AKIZUKI, Akiko Hata, Akira Tangoku, Hiromichi Yumoto, Hirohisa Ogawa and Takeshi Oya : 休眠状態の導入・維持による脂肪組織由来間葉系幹細胞の若返りを通じた，糖尿病性合併症治療への応用の可能性, Journal of the The Japan Diabetes Society, Vol.65, No.Suppl.1, S257, May 2022.
6.	Rie Kido, Eijiro Sakamoto, Yuka Hiroshima, Mika Bandou, Jun-ichi Kido, Takahisa Ikuta, Masami Ninomiya, Yuji Inagaki, Yasufumi Nishikawa, Yoritoki Tomotake, Koji Naruishi and Hiromichi Yumoto : イムノクロマト法を用いたカルプロテクチン測定による歯周病およびインプラント疾患の診断, 四国歯学会第59回例会, Mar. 2022.
7.	Masami Ninomiya, Yuta Uemura, Minato Akizuki, Takahisa Ikuta and Hiromichi Yumoto : A case report of necrortizing ulcerative gingivitis that became severe after the common cold, 第64回秋季日本歯周病学会学術大会プログラムおよび演題抄録集, Vol.63, 161, Oct. 2021. (Keyword) Necrortizing ulcerative gingivitis / 免疫機能低下 / Common cold / 接触障害
8.	Takahisa Ikuta, Masami Ninomiya, 秋月皆人, Yuta Uemura, Koji Naruishi and Hiromichi Yumoto : Examination of the effectiveness of Porphyromonas gingivalis type 2 fimbria detection system by PCR, 第64回春季日本歯周病学会学術大会プログラムおよび演題抄録集, Vol.63, 24, May 2021. (Keyword) PCR / Porphyromonas gingivalis
9.	AKIZUKI Minato, Masami Ninomiya, Takahisa Ikuta, Yuta Uemura and Hiromichi Yumoto : チェアサイドでのPorphyromonas gingivalis迅速検出システムの有用性, 四国歯学会第57回例会, Mar. 2021. (Keyword) PCR検査 / 歯周病原細菌 / Porphyromonas gingivalis
10.	AKIZUKI Minato, Masami Ninomiya, Yuta Uemura, Takahisa Ikuta, Koji Naruishi and Hiromichi Yumoto : Usefulness of rapid detection system of Porphyromonas gingivalis using PCR, 日本歯科保存学会第153回 2020年度秋季学術大会, Nov. 2020. (Keyword) periodontitis / Porphyromonas gingivalis / PCR / clinical laboratory testing
11.	Masami Ninomiya, Yuta Uemura, Akihiro Wada, Takahisa Ikuta and Hiromichi Yumoto : A case report of the periodontal regeneration therapy in combination with REGROTH and CytransGranules for a severe bone defect in mandibular first molar, Journal of the Japanese Association of Periodontology, Vol.62, 134, Oct. 2020. (Keyword) Periodontal regeneration therapy / Severe periodontitis / REGROTH / CytransGranules
12.	Rie Kido, Yuka Hiroshima, Takahisa Ikuta, Yuji Inagaki, Mika Bandou, Koji Naruishi, Jun-ichi Kido and Hiromichi Yumoto : 最終糖化産物による口腔上皮細胞の Lipocalin2 発現誘導は好中球の遊走性とサイトカイン発現を調節する, 第63回春季日本歯周病学会学術大会, May 2020.
13.	植村勇太, Masami Ninomiya, 和田明大, Rie Kido, 高木亮輔, 野中康平, 谷口裕哉, Takahisa Ikuta, Eijiro Sakamoto, Mika Bandou, Yuji Inagaki, Koji Naruishi, Jun-ichi Kido and Hiromichi Yumoto : 歯周病ならびに歯周組織再生療法に関する臨床研究について, 徳島県歯科医学大会・四国歯学会第56回例会, Feb. 2020.
14.	Masami Ninomiya, Takahisa Ikuta, Koji Naruishi and Hiromichi Yumoto : 重度慢性歯周炎を有する血液透析患者に対して包括的歯周治療を行った一症例, 日本歯科保存学会 2019年度秋季学術大会(第151回), Nov. 2019. (Keyword) periodontal disease / dialysis / Periodontal treatment / Periodontal medicine
15.	Yuji Inagaki, Takahisa Ikuta, Eijiro Sakamoto, Jun-ichi Kido and Hiromichi Yumoto : 全顎的歯周治療に伴って関節リウマチの状態が改善した一症例, 日本歯科保存学会2019年度春季学術大会(第150回), Jun. 2019.
16.	Masami Ninomiya, Eijiro Sakamoto, Koji Naruishi, Takahisa Ikuta, Ryosuke Takagi, Hatanaka Kazu, Okamoto Kentaro, Ono Shintaro, Takashiba Shogo and Hiromichi Yumoto : A Familial Case of Severe Periodontitis associated with Cyclic Neutropenia, Journal of the Japanese Association of Periodontology, Vol.61, 164, May 2019. (Keyword) Cyclic Neutropenia / Familial / Severe Periodontitis
17.	Rie Kido, Yuka Hiroshima, Takahisa Ikuta, Kaya Yoshida, Yuji Inagaki, Koji Naruishi, Kazumi Ozaki, Jun-ichi Kido and Hiromichi Yumoto : 最終糖化産物はヒト口腔上皮細胞のリポカリン2発現を増加する, 第62回春季日本歯周病学会学術大会, May 2019.
18.	Rie Kido, Eijiro Sakamoto, Mika Bandou, Yuji Inagaki, Masami Ninomiya, Chie Wada -Mihara, Takahisa Ikuta, Koji Naruishi, Toshihiko Nagata, Jun-ichi Kido and Hiromichi Yumoto : インプラント周囲溝滲出液中のカルプロテクチンとNTxを用いたインプラント疾患の診断, 第61回春季日本歯周病学会学術大会, Jun. 2018.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

Study of exosomes from cells in periodontal tissues with diabetes-associated periodontitis (Project/Area Number: 21K09895 )
スクレロスチンを新規標的マーカーとした糖尿病関連歯周炎の非侵襲的診断法の開発 (Project/Area Number: 21K09874 )
Relationship between periodontal pathogen and estimated glomerular filtration rate (eGFR) in patients with chronic kidney disease (Project/Area Number: 19K10111 )
Study of effects of advanced glycation end-products and LPS under hypoxia on the pathological condition of diabetes-associated periodontitis (Project/Area Number: 18K17051 )
The effect of calprotectin on mechanism of progression of diabetes-associated periodontitis (Project/Area Number: 15K20621 )
Search by Researcher Number (00746563)

Assistant Professor : Ikuta, Takahisa

Research

Personal Web Page

Field of Study

Subject of Study

Book / Paper

Book:

Academic Paper (Judged Full Paper):

Proceeding of International Conference:

Proceeding of Domestic Conference:

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)