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en: Effects of Candidalysin Derived from Candida albicans on the Expression of Pro-Inflammatory Mediators in Human Gingival Fibroblasts

en: International Journal of Molecular Sciences


en: Diagnosis of inflammatory peri-implant diseases using an immunochromatographic assay for calprotectin in peri-implant crevicular fluid

en: The incidence rate of peri-implant diseases is increasing with implant placement. Early detection of peri-implant diseases is important to prevent and treat these diseases, and a simple and objective diagnostic method is expected. Immunochromatographic (IC) assays are used for rapid diagnostic methods for some diseases. The aim of this clinical study was to determine the amount of calprotectin, an inflammatory marker, in peri-implant crevicular fluid (PICF) using an IC chip, and estimate the possibility of this diagnostic system. Forty-six individuals with dental implants participated in a pilot study. PICF samples were collected from the peri-implant sites with or without inflammation after clinical examinations including probing depth (PD), bleeding on probing (BOP) and gingival index (GI). Calprotectin in PICF was determined by an IC chip and enzyme-linked immunosorbent assay (ELISA) for calprotectin. The density of calprotectin line on the IC chip was measured using an IC reader (IC reader value). The relationship between IC reader value and ELISA value or clinical parameters was investigated. A receiver operating characteristic (ROC) curve analysis of IC reader value of calprotectin was performed to predict inflammation in peri-implant diseases. IC reader value of calprotectin was significantly correlated with its ELISA value and PD. IC reader values of calprotectin in PICF samples from periodontal sites with GI-1 and GI-2, and with BOP-positive sites were significantly higher than those of PICF samples from GI-0 sites, and BOP-negative sites, respectively. The IC reader value for calprotectin in PICF samples from inflammatory diseased sites was significantly higher than that of non-diseased sites. ROC analysis suggested that the IC reader value of PICF calprotectin was useful for predicting inflammatory peri-implant diseases. IC assay for PICF calprotectin may be a possible system for diagnosing the inflammatory peri-implant diseases.

en: International Journal of Implant Dentistry


en: Gan-Lu-Yin (Kanroin), traditional Chinese herbal extracts, reduces osteoclast differentiation in vitro and prevents alveolar bone resorption in rat experimental periodontitis

en: Gan-Lu-Yin (GLY), a traditional Chinese herbal medicine, shows therapeutic effects on periodontitis, but that mechanism is not well known. This study aims to clarify the precise mechanism by investigating the inhibitory effects of GLY extracts on osteoclastogenesis in vitro and on bone resorption in periodontitis in vivo. RAW264.7 cells are cultured with soluble receptor activator of nuclear factor-kappa B (sRANKL) and GLY extracts (0.01-1.0 mg/mL), and stained for tartrate-resistant acid phosphatase (TRAP) to evaluate osteoclast differentiation. Experimental periodontitis is induced by placing a nylon ligature around the second maxillary molar in rats, and rats are administered GLY extracts (60 mg/kg) daily for 20 days. Their maxillae are collected on day 4 and 20, and the levels of alveolar bone resorption and osteoclast differentiation are estimated using micro-computed tomography (CT) and histological analysis, respectively. In RAW264.7 cells, GLY extracts significantly inhibit sRANKL-induced osteoclast differentiation at a concentration of more than 0.05 mg/mL. In experimental periodontitis, administering GLY extracts significantly decreases the number of TRAP-positive osteoclasts in the alveolar bone on day 4, and significantly inhibits the ligature-induced bone resorption on day 20. These results show that GLY extracts suppress bone resorption by inhibiting osteoclast differentiation in experimental periodontitis, suggesting that GLY extracts are potentially useful for oral care in periodontitis.

en: Journal of Clinical Medicine

ja: Journal of Clinical Medicine


en: Clinical Statistical Study of New Patients in Oral Implant Center at Tokushima University Hospital

ja: 徳島大学病院口腔インプラントセンターにおける新来患者の臨床統計

en:
Purpose: Oral Implant Center established in April 2014 as specialized clinical section at Tokushima University Hospital. The aim of this study was to report a survey of new patients of preoperative registration at Oral Implant Center during the five years since its establishment.
 Subjects and methods: Investigation of gender and age, chief complaint, details of treatment requests, sites of missing teeth, number of missing teeth, cause of tooth extraction, and treatment status for patients registered at Oral Implant Center from April 2014 to March 2019 were performed.
 Results: Regarding the chief complaint, 605 (88.3%) patients consulted preoperatively for a request for implant treatment, and 80 (11.7%) consulted about complain of previous implant treatment or a request for continued maintenance. The average number of missing teeth for which treatment was requested was 3.8. The proportion of patients who wished to be treated for the number of missing teeth was 32.2% for missing one tooth, 56.1% for missing few teeth (2 to 6 teeth), 7.8% for missing many teeth (7 or more teeth) and edentulous 3.9%. As for the details of treatment requests of 80 patients who had already received implant treatment, 33 (41.3%) consulted on the improper superstructure, 29 (36.3%) wished to examine and treat peri-implantitis, 4 (5%) wished to remove the implant and 14 (17.5%) requested continued maintenance.
 Discussion and conclusion: The number of new patients regarding implant treatment has been about the same each year, and it was reconfirmed that it was recognized as a treatment method for dental prostheses. In particular, many patients wished to receive implant treatment for a single tooth missing, suggesting that the patients also recognized the importance of preserving the remaining teeth. On the other hand, the number of patients complaining of previous implant treatment tends to increase, and it is necessary to consider future correspondence.

en: Journal of Oral Health and Biosciences

ja: Journal of Oral Health and Biosciences


en: Inhibitory Effects of Sudachitin on the Production of Inflammation-related Molecules in Human Gingival Fibroblasts Cultured under High Glucose Conditions

ja: 高グルコース条件下における歯肉線維芽細胞のカルプロテクチン誘導性炎症関連因子の産生におけるスダチチンの抑制効果

en: The Japanese Journal of Conservative Dentistry

ja: 日本歯科保存学雑誌


en: Relationship of aspiration pneumonia to cognitive impairment and oral condition: a cross-sectional study.

en: Early and simple evaluation of the oral condition and cognitive function can predict the risk of aspiration pneumonia.

en: Clinical Oral Investigations

ja: Clinical Oral Investigations


en: Calprotectin Induces IL-6 and MCP-1 Production via Toll-Like Receptor 4 Signaling in Human Gingival Fibroblasts.

en: Calprotectin, a heterodimer of S100A8 and S100A9 molecules, is associated with inflammatory diseases such as inflammatory bowel disease. We have reported that calprotectin levels in gingival crevicular fluids of periodontitis patients are significantly higher than in healthy subjects. However, the functions of calprotectin in pathophysiology of periodontitis are still unknown. The aim of this study is to investigate the effects of calprotectin on the productivity of inflammatory cytokines in human gingival fibroblasts (HGFs). The HGFs cell line CRL-2014® (ATCC) were cultured, and total RNAs were collected to examine the expression of TLR2/4 and RAGE mRNA using RT-PCR. After the cells were treated with S100A8, S100A9, and calprotectin, supernatants were collected and the levels of IL-6 and MCP-1 were measured using ELISA methods. To examine the intracellular signals involved in calprotectin-induced cytokine production, several chemical inhibitors were used. Furthermore, after the siRNA-mediated TLR4 down-regulated cells were treated with S100A8, S100A9, and calprotectin, the levels of IL-6 and MCP-1 were also measured. HGFs showed greater expression of TLR4 mRNA, but not TLR2 and RAGE mRNA compared with human oral epithelial cells. Calprotectin increased significantly the production of MCP-1 and IL-6 in HGFs, and the cytokine productions were significantly suppressed in the cells treated with MAPKs, NF-B, and TLR4 inhibitors. Furthermore, calprotectin-mediated MCP-1 and IL-6 production were significantly suppressed in TLR4 down-regulated cells. Taken together, calprotectin induces IL-6 and MCP-1 production in HGFs via TLR4 signaling that involves MAPK and NF-B, resulting in the progression of periodontitis. J. Cell. Physiol. 232: 1862-1871, 2017. © 2016 Wiley Periodicals, Inc.

en: Journal of Cellular Physiology

ja: Journal of Cellular Physiology