fumiya/published_papers/52697103 Yang Xu Noboru Hashimoto Fumiya Kano Linze Xia Cheng Ding Houjun Lao Yifei Wang Takahiro Arai Tomonori Iwasaki Akihito Yamamoto Conditioned medium from stem cells of human exfoliated deciduous teeth ameliorates mouse ovalbumin-specific allergic dermatitis. BACKGROUND: Atopic dermatitis (AD), a chronic inflammatory skin disease, is characterized by intense itching and recurrent eczema-like lesions, requiring safer, more targeted therapies that modulate the immune response and restore skin barrier integrity. Conditioned medium derived from stem cells of human exfoliated deciduous teeth (SHED-CM) exhibits considerable immunomodulatory and regenerative effects. We evaluated the efficacy of SHED-CM in an AD-like mouse model and explored the underlying mechanisms. METHODS: Mice with ovalbumin (OVA)-specific allergic dermatitis (OSAD) were intravenously treated with SHED-CM, conditioned medium derived from fibroblast, or Dulbecco's Modified Eagle Medium, followed by antigen restimulation to induce AD-like dermatitis. Disease severity was assessed macroscopically and histologically. T cell phenotypes were analyzed using immunohistochemical staining, RT-qPCR, and flow cytometry. Total serum and OVA-specific immunoglobulin E (IgE) levels were measured using ELISA. Naïve splenic CD4⁺ T cells were activated using CD3/CD28 beads in SHED-CM to evaluate their direct effects on T cell differentiation through flow cytometry. Highly expressed proteins in SHED-CM were identified using liquid chromatography-mass spectrometry, and neutralizing antibodies were used to elucidate the therapeutic mechanisms. RESULTS: The treatment with SHED-CM significantly ameliorated AD-like symptoms, reduced epidermal hyperplasia, and restored filaggrin expression. SHED-CM created an anti-inflammatory microenvironment in OSAD skin and draining lymph nodes by promoting Treg differentiation and inhibiting Th2 and Th17 populations. SHED-CM markedly suppressed B cell maturation, antigen-specific IgE production, and mast cell activation. Furthermore, we found that SHED-CM directly promoted Treg differentiation from naïve CD4⁺ T cells stimulated by CD3/CD28, which was mediated, in part, through TGFβ contained in SHED-CM. SHED-CM exhibited little to no effect on in vitro Th1, Th2, and Th17 differentiation. CONCLUSIONS: SHED-CM shows significant therapeutic potential for AD-like symptom, effectively modulating immune response and enhancing skin barrier restoration. Our findings offer a new approach for developing novel, targeted therapies for inflammatory skin disorders. Stem cell research & therapy 16 1 605 605 20251031 10.1186/s13287-025-04735-2 41174821 fumiya/published_papers/49061581 Cheng Ding Noboru Hashimoto Fumiya Kano Hirofumi Tenshin Takahiro Arai Linze Xia Yang Xu Houjun Lao Yifei Wang Tomonori Iwasaki Hideharu Hibi Akihito Yamamoto Factors secreted from the stem cells of human exfoliated deciduous teeth inhibit osteoclastogenesis through the activation of the endogenous antioxidant system. OBJECTIVES: Systemic administration of conditioned medium (CM) from stem cells derived from human exfoliated deciduous teeth (SHED-CM) in mouse models of rheumatoid arthritis, osteoporosis, and osteoarthritis suppresses excessive osteoclast activity and restores bone integrity. However, the mechanism through which SHED-CM regulates osteoclastogenesis remains largely unknown. In the present study, we examined the anti-osteoclastogenic mechanism of SHED-CM in vitro. METHODS: Bone marrow macrophages and RAW264.7 cells were treated with receptor activator of nuclear factor kappa-Β ligand (RANKL) in the presence of SHED-CM or CM from bone marrow mesenchymal stem cells (BMSC-CM). Osteoclast differentiation was assessed using tartrate-resistant acid phosphatase staining, actin ring formation, and expression of osteoclast-specific markers. RANKL-induced reactive oxygen species (ROS) production was analyzed as a critical mediator of osteoclastogenesis. The activation of endogenous antioxidant gene expression was examined using reverse transcription quantitative PCR. Liquid chromatography with tandem mass spectrometry (LC-MS) was used to identify proteins enriched in SHED-CM, and neutralizing antibodies were used to evaluate their functional roles. RESULTS: Compared to BMSC-CM, SHED-CM effectively inhibited RANKL-induced early osteoclast differentiation and late maturation. Notably, SHED-CM but not BMSC-CM suppressed RANKL-induced ROS production. SHED-CM increased the expression of genes encoding antioxidant enzymes. The LC-MS analysis identified seven proteins uniquely enriched in SHED-CM that activated the endogenous antioxidant system. Neutralizing antibodies against some of these proteins restore RANKL-induced ROS production and osteoclast differentiation. CONCLUSIONS: SHED-CM inhibited osteoclastogenesis, partially through the activation of multiple antioxidant enzymes in osteoclast precursors, highlighting its potential for treating bone-destructive diseases. Journal of oral biosciences 100618 100618 20250122 10.1016/j.job.2025.100618 39855425 fumiya/published_papers/48276577 Linze Xia Fumiya Kano Noboru Hashimoto Yao Liu Tsendsuren Khurel-Ochir Naoko Ogasawara Cheng Ding Yang Xu Hideharu Hibi Tomonori Iwasaki Eiji Tanaka Akihito Yamamoto Conditioned Medium From Stem Cells of Human Exfoliated Deciduous Teeth Alleviates Mouse Osteoarthritis by Inducing sFRP1-Expressing M2 Macrophages. Intravenous administration of conditioned medium from stem cells of human exfoliated deciduous teeth (SHED-CM) regenerates mechanically injured osteochondral tissues in mouse temporomandibular joint osteoarthritis (TMJOA). However, the underlying therapeutic mechanisms remain unclear. Here, we showed that SHED-CM alleviated injured TMJ by inducing anti-inflammatory M2 macrophages in the synovium. Depletion of M2 by Mannosylated Clodrosome abolished the osteochondral repair activities of SHED-CM. Administration of CM from M2-induced by SHED-CM (M2-CM) effectively ameliorated mouse TMJOA by inhibiting chondrocyte inflammation and matrix degradation while enhancing chondrocyte proliferation and matrix formation. Notably, in vitro, M2-CM directly suppressed the catabolic activities while enhancing the anabolic activities of interleukin-1β-stimulated mouse primary chondrocytes. M2-CM also inhibited receptor activator of nuclear factor NF-κB ligand-induced osteoclastogenesis in RAW264.7 cells. Secretome analysis of M2-CM and M0-CM revealed that 5 proteins related to anti-inflammation and/or osteochondrogenesis were enriched in M2-CM. Of these proteins, the Wnt signal antagonist, secreted frizzled-related protein 1 (sFRP1), was the most abundant and played an essential role in the shift to anabolic chondrocytes, suggesting that M2 ameliorated TMJOA partly through sFRP1. This study suggests that secretome from SHED exerted remarkable osteochondral regeneration activities in TMJOA through the induction of sFRP1-expressing tissue-repair M2 macrophages. Stem cells translational medicine 13 4 399 413 20240415 10.1093/stcltm/szae006 38366885 fumiya/published_papers/41509418 Fumiya Kano Noboru Hashimoto Yao Liu Linze Xia Takaaki Nishihara Wakana Oki Keita Kawarabayashi Noriko Mizusawa Keiko Aota Takayoshi Sakai Masayuki Azuma Hideharu Hibi Tomonori Iwasaki Tsutomu Iwamoto Nobuyasu Horimai Akihito Yamamoto Therapeutic benefits of factors derived from stem cells from human exfoliated deciduous teeth for radiation-induced mouse xerostomia. Radiation therapy for head and neck cancers is frequently associated with adverse effects on the surrounding normal tissue. Irreversible damage to radiation-sensitive acinar cells in the salivary gland (SG) causes severe radiation-induced xerostomia (RIX). Currently, there are no effective drugs for treating RIX. We investigated the efficacy of treatment with conditioned medium derived from stem cells from human exfoliated deciduous teeth (SHED-CM) in a mouse RIX model. Intravenous administration of SHED-CM, but not fibroblast-CM (Fibro-CM), prevented radiation-induced cutaneous ulcer formation (p < 0.0001) and maintained SG function (p < 0.0001). SHED-CM treatment enhanced the expression of multiple antioxidant genes in mouse RIX and human acinar cells and strongly suppressed radiation-induced oxidative stress. The therapeutic effects of SHED-CM were abolished by the superoxide dismutase inhibitor diethyldithiocarbamate (p < 0.0001). Notably, quantitative liquid chromatography-tandem mass spectrometry shotgun proteomics of SHED-CM and Fibro-CM identified eight proteins activating the endogenous antioxidant system, which were more abundant in SHED-CM than in Fibro-CM (p < 0.0001). Neutralizing antibodies against those activators reduced antioxidant activity of SHED-CM (anti-PDGF-D; p = 0.0001, anti-HGF; p = 0.003). Our results suggest that SHED-CM may provide substantial therapeutic benefits for RIX primarily through the activation of multiple antioxidant enzyme genes in the target tissue. Scientific reports 13 1 2706 2706 20230215 10.1038/s41598-023-29176-w 36792628 fumiya/published_papers/48276581 XIA Linze KANO Fumiya HASHIMOTO Noboru DING Cheng XU Yang HIBI Hideharu IWASAKI Tomonori TANAKA Eiji YAMAMOTO Akihito Conditioned Medium from Stem Cells of Human Exfoliated Deciduous Teeth Partially Alters the Expression of Inflammation-associated Molecules of Mouse Condylar Chondrocytes via Secreted Frizzled-related Protein 1 Intravenous administration of conditioned medium from stem cells of human exfoliated deciduous teeth (SHED-CM) effectively restores mechanically injured osteochondral tissues in mouse temporomandibular joint osteoarthritis. However, the underlying therapeutic mechanisms remain elusive. Here, we investigated the direct therapeutic effects of SHED-CM on inflamed primary condylar chondrocytes <i>in vitro</i>. Immunofluorescence staining revealed that interleukin-1β-stimulated chondrocytes showed increased expression of the catabolic marker inducible nitric oxide synthase (iNOS) and reduced expression of the anabolic marker aggrecan (ACAN). We found that SHEDCM treatment, but not conditioned medium from bone marrow mesenchymal stem cells (BMSCCM), effectively suppressed iNOS expression and elevated ACAN levels, indicating that SHEDCM converted the catabolic phenotype of inflamed chondrocytes to an anabolic phenotype. Liquid chromatography with tandem mass spectrometry analysis of SHED-CM and BMSC-CM identified eight proteins enriched in SHED-CM that are related to anti-inflammatory and/or chondrogenic processes. Of these proteins, the Wnt signal inhibitor secreted frizzled-related protein 1 (SFRP1) was the most abundantly enriched in SHED-CM. We found that treatment with the selective SFRP1 inhibitor WAY-316606 abolished the anti-catabolic and pro-anabolic effects of SHED-CM. Collectively, our study suggests that SHED-CM directly suppresses catabolism and promotes anabolic responses of inflamed primary condylar chondrocytes, which partially rely on SFRP1 function in SHED-CM. The direct action of SHED-CM may be useful to treat inflammatory cartilage diseases. Shikoku Society of Dental Research Journal of Oral Health and Biosciences 2189-6682 35 2 52 60 20230000 10.20738/johb.35.2_52 1390295568860792704 fumiya/published_papers/36668795 Yao Liu Fumiya Kano Noboru Hashimoto Linze Xia Qiao Zhou Xingmei Feng Hideharu Hibi Aya Miyazaki Tsutomu Iwamoto Yoshizo Matsuka Zhijun Zhang Eiji Tanaka Akihito Yamamoto Conditioned Medium From the Stem Cells of Human Exfoliated Deciduous Teeth Ameliorates Neuropathic Pain in a Partial Sciatic Nerve Ligation Model In neuropathic pain (NP), injury or diseases of the somatosensory system often result in highly debilitating chronic pain. Currently, there is no effective drug for the complete and definitive treatment of NP. We investigated the therapeutic potential of conditioned medium (CM) derived from stem cells from human exfoliated deciduous teeth (SHED-CM) against NP using a mouse partial sciatic nerve ligation (PSL) model. Abnormal pain sensation, such as tactile allodynia and hyperalgesia, can be caused by PSL. In the behavioral test, intravenous administration of SHED-CM greatly improved the PSL-induced hypersensitivity. We found that treatment with SHED-CM resulted in the recruitment of M2 macrophages in the injured sciatic nerve and ipsilateral L4/L5 dorsal root ganglion and suppressed microglial activation in the spinal cord. Notably, specific depletion of the anti-inflammatory M2 macrophages by mannosylated-Clodrosome markedly reduced the antinociceptive effect of SHED-CM. Intravenous administration of CM from M2 induced by SHED-CM (M2-CM) ameliorated the PSL-induced hypersensitivity. We found that M2-CM directly suppressed the expression of nociceptive receptors as well as proinflammatory mediators in Schwann cells. Taken together, our data suggest that SHED-CM ameliorates NP through the induction of the analgesic anti-inflammatory M2 macrophages. Thus, SHED-CM may be a novel therapeutic candidate for NP. Frontiers Media SA Frontiers in Pharmacology 1663-9812 13 745020 745020 20220331 10.3389/fphar.2022.745020 35431971 fumiya/published_papers/33941221 Hisanori Muto Takanori Ito Taku Tanaka Shinya Yokoyama Kenta Yamamoto Norihiro Imai Yoji Ishizu Keiko Maeda Takashi Honda Tetsuya Ishikawa Asuka Kato Taichi Ohshiro Fumiya Kano Akihito Yamamoto Kiyoshi Sakai Hideharu Hibi Masatoshi Ishigami Mitsuhiro Fujishiro Conditioned medium from stem cells derived from human exfoliated deciduous teeth ameliorates NASH via the Gut-Liver axis <title>Abstract</title>Non-alcoholic steatohepatitis (NASH) occurrence has been increasing and is becoming a major cause of liver cirrhosis and liver cancer. However, effective treatments for NASH are still lacking. We examined the benefits of serum-free conditioned medium from stem cells derived from human exfoliated deciduous teeth (SHED-CM) on a murine non-alcoholic steatohepatitis (NASH) model induced by a combination of Western diet (WD) and repeated administration of low doses of carbon tetrachloride intraperitoneally, focusing on the gut-liver axis. We showed that repeated intravenous administration of SHED-CM significantly ameliorated histological liver fibrosis and inflammation in a murine NASH model. SHED-CM inhibited parenchymal cell apoptosis and reduced the activation of inflammatory macrophages. Gene expression of pro-inflammatory and pro-fibrotic mediators (such as <italic>Tnf-α</italic><italic>, </italic><italic>Tgf-β,</italic> and <italic>Ccl-2</italic>) in the liver was reduced in mice treated with SHED-CM. Furthermore, SHED-CM protected intestinal tight junctions and maintained intestinal barrier function, while suppressing gene expression of the receptor for endotoxin, <italic>Toll-like receptor 4</italic>, in the liver. SHED-CM promoted the recovery of Caco-2 monolayer dysfunction induced by IFN-γ and TNF-α in vitro. Our findings suggest that SHED-CM may inhibit NASH fibrosis via the gut-liver axis, in addition to its protective effect on hepatocytes and the induction of macrophages with unique anti-inflammatory phenotypes. Springer Science and Business Media LLC Scientific Reports 2045-2322 11 1 18778 18778 20211200 10.1038/s41598-021-98254-8 34548598 fumiya/published_papers/44411248 坂巻 拓馬 岩浅 亮彦 小笠原 直子 加納 史也 山本 朗仁 田中 栄二 変形性顎関節症に対する低出力超音波パルス照射の治療効果 (公社)日本矯正歯科学会 日本矯正歯科学会大会プログラム・抄録集 80 139 139 20211100 fumiya/published_papers/36263907 Arief Waskitho Yumiko Yamamoto Swarnalakshmi Raman Fumiya Kano Huijiao Yan Resmi Raju Shaista Afroz Tsuyoshi Morita Daisuke Ikutame Kazuo Okura Masamitsu Oshima Akihito Yamamoto Otto Baba Yoshizo Matsuka Peripherally Administered Botulinum Toxin Type A Localizes Bilaterally in Trigeminal Ganglia of Animal Model MDPI AG Toxins 2072-6651 13 10 704 704 20211005 10.3390/toxins13100704 34678997 2-s2.0-85117193943 fumiya/published_papers/49686984 坂巻 拓馬 岩浅 亮彦 Xia Linze 井澤 俊 加納 史也 山本 朗仁 田中 栄二 低出力超音波パルス照射は機械的負荷に起因して生じた下顎頭軟骨変性を修復する (一社)日本顎関節学会 日本顎関節学会雑誌 1884-4308 33 Suppl. 80 80 20211000 fumiya/published_papers/33940488 Tsendsuren Khurel-Ochir Takashi Izawa Akihiko Iwasa Fumiya Kano Akihito Yamamoto Eiji Tanaka The immunoregulatory role of p21 in the development of the temporomandibular joint‐osteoarthritis Wiley Clinical and Experimental Dental Research 2057-4347 7 3 313 322 20210600 10.1002/cre2.404 fumiya/published_papers/48276582 LIU Yao XIA Linze KANO Fumiya HASHIMOTO Noboru MATSUKA Yoshizo YAMAMOTO Akihito TANAKA Eiji Low-Intensity Pulsed Ultrasound Ameliorates Neuropathic Pain Induced by Partial Sciatic Nerve Ligation Via Regulating Macrophage Polarization Inflammatory (M1-polarized) macrophages cause neuropathic pain (NP) after nerve injury through non-resolving neuroinflammation. However, increasing evidence suggests that converting M1 to anti-inflammatory M2 macrophages may rescue NP. In the present study, the therapeutic potential of low-intensity pulsed ultrasound (LIPUS) was investigated in a partial sciatic nerve ligation (PSL)- induced NP model.<br> <b>Materials and Methods:</b> Abnormal pain sensation, such as tactile allodynia, was caused by PSL. Immediately after PSL induction, the mice were subjected to LIPUS treatment for 20 min/day for 7 days. LIPUS was used at an average intensity of 60 mW/cm² and a frequency of 1.5 MHz.<br> <b>Results:</b> In the behavioral test, the LIPUS group showed a significant improvement in the PSL-induced hypersensitivity compared to the PSL group not exposed to LIPUS. We found an increasing number of M2 macrophages in the injured sciatic nerves after LIPUS exposure. LIPUS treatment decreased expression of pro-inflammatory microglial markers in spinal cord.<br> <b>Conclusions:</b> Our data suggest that LIPUS has an anti-nociceptive effect by increasing anti-inflammatory M2 macrophage and may be a suitable therapeutic candidate for NP. Shikoku Society of Dental Research Journal of Oral Health and Biosciences 2189-6682 34 1 11 18 20210000 10.20738/johb.34.1_11 1390570784697431168 fumiya/published_papers/27745114 Eiji Tanaka Yao Liu Linze Xia Naoko Ogasawara Takuma Sakamaki Fumiya Kano Noboru Hashimoto Xingmei Feng Akihito Yamamoto Effectiveness of low-intensity pulsed ultrasound on osteoarthritis of the temporomandibular joint: A review Springer Science and Business Media LLC Annals of Biomedical Engineering 1573-9686 null null 20200608 10.1007/s10439-020-02540-x fumiya/published_papers/27745656 Keita KAWARABAYASHI Fumiya KANO Noboru HASHIMOTO Hideharu HIBI Tsutomu IWAMOTO Akihito YAMAMOTO Conditioned Media from Human Dental Pulp Stem Cells Prevent Radiation-induced Skin Injury Journal of Oral Health and Biosciences 33 1 1 7 20200600 fumiya/published_papers/27745222 N. Ogasawara F. Kano N. Hashimoto H. Mori Y. Liu L. Xia T. Sakamaki H. Hibi T. Iwamoto E. Tanaka A. Yamamoto Factors secreted from dental pulp stem cells show multifaceted benefits for treating experimental temporomandibular joint osteoarthritis Elsevier BV Osteoarthritis and Cartilage 1522-9653 28 6 831 841 20200600 10.1016/j.joca.2020.03.010 32272195 2-s2.0-85083636920 fumiya/published_papers/27745020 Kazuto Okabe Ryuji Kaneko Takamasa Kawai Fumiya Kano Yuya Ohta Hideharu Hibi Oral self-injury associated with septicaemia in a case of Cornelia de Lange syndrome Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology 2212-5566 32 2 136 139 20200300 10.1016/j.ajoms.2019.10.003 fumiya/published_papers/23497791 Jun Ishikawa Fumiya Kano Yuji Ando Hideharu Hibi Akihito Yamamoto Monocyte chemoattractant protein-1 and secreted ectodomain of sialic acid- T binding Ig-like lectin-9 enhance bone regeneration by inducing M2 macrophages Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology 2212-5566 31 3 169 174 20190000 10.1016/j.ajoms.2018.12.007 fumiya/published_papers/23497790 市村 典久 山本 憲幸 西川 雅也 山口 聡 加納 史也 日比 英晴 顎下腺に発生したびまん性大細胞型B細胞リンパ腫を含むMALTリンパ腫の1例 症例は67歳女性で，右側顎下部の腫脹を自覚し，経過観察となった．その後も症状の改善を認めなかった．MRI所見では，右側顎下腺内に31×24mm大の病変を認めた．T2強調STIR像では，淡い高信号で辺縁に索状の低信号域を認め，全体として分葉状の像を呈した．CT所見では，水平断で右側下顎骨内側から右側舌骨外側に及ぶ約30mm大の類円形の腫瘤性病変を認めた．矢状断では下方は舌骨下縁から上方は顎舌骨筋線付近に及ぶ約30mm大の腫瘤性病変を認めた．唾液腺シンチグラフィー所見では，99mTcO4-の右側顎下腺への集積は上方に変形して認めた．超音波断層検査所見では，右側顎下腺内に31×21×20mm大の比較的境界明瞭，内部やや不均一の腫瘤を認めた．超音波エコーガイド下にFNA(Fine needle Aspiration)生検を実施したが，良性・悪性の判断は困難であった．全身麻酔下にて右側顎下腺腫瘍摘出術を施行し，び漫性大細胞型B細胞リンパ腫(DLBCL)を一部に含むMALTリンパ腫との確定診断を得た．R-CHOP療法を施行し，右側頸部から顎下部にかけて放射線照射を行った．治療後2年1ヵ月経過した現在，経過良好で再発は認めていない． (公社)日本口腔外科学会 日本口腔外科学会雑誌 0021-5163 64 8 470 474 20180800 fumiya/published_papers/17954046 Ichimura N Yamamoto N Nishikawa M Sakakura H Kano F Sato K Yamaguchi S Hibi H Human papillomavirus related carcinogenesis in oral cancer Oral Science in Japan 2017 2017 83 84 20180400 fumiya/published_papers/23497792 Fumiya Kano Norihisa Ichimura Yukiko Wakayama Kazuto Okabe Hiroki Sakakura Hideharu Hibi Third molar pericoronitis in neutropenia IDCASES 2214-2509 13 2018 e00419 null 20180000 10.1016/j.idcr.2018.e00419 fumiya/published_papers/28383015 山口 聡 山本 憲幸 伊藤 善之 西川 雅也 市村 典久 加納 史也 日比 英晴 半導体レーザーを用いた扁平上皮癌の治療効果についての検討 (一社)日本ハイパーサーミア学会 Thermal Medicine 1882-3750 33 3 92 92 20171000 fumiya/published_papers/28383017 山本 憲幸 山口 聡 西川 雅也 市村 典久 加納 史也 伊藤 義之 日比 英晴 扁平上皮癌に対する半導体レーザーを用いた温熱療法 (一社)日本ハイパーサーミア学会 Thermal Medicine 1882-3750 33 Suppl. 120 120 20170900 fumiya/published_papers/23497794 Tohru Tanigawa Hirokazu Tanaka Fumiya Kano Hiromi Ueda Shigeru Inafuku Nasal hemangiopericytoma successfully treated with a combination of rIL-2 and extranasal approaches Journal of Surgical Case Reports 10 2017 1 3 20170900 fumiya/published_papers/27916326 Yuichiro Horibe Tohru Tanigawa Rei Shibata Hiroshi Nonoyama Fumiya Kano Satoshi Yamaguchi Kenta Murotani Takaki Ogawa Hiromi Ueda Efficacy of the red blood cell distribution width for predicting the prognosis of Bell palsy: a pilot study. The aim of this study was to investigate the association between RDW values and the prognosis of patients with Bell palsy in an effort to find a prognostic biomarker that predicts recovery from Bell palsy. We measured RDW and evaluated facial movement in 61 patients with Bell palsy aged 50 years and less. All patients were treated with a steroid plus an antiviral agent. Seven patients underwent surgery for facial nerve decompression. During the post-treatment period, patients with a Yanagihara grading score of 36 or more were regarded as having a satisfactory recovery. Patients were divided into two groups (recovered and unrecovered) according to their response to treatment, and several parameters, including the RDW, were measured for further analysis. RDW values were significantly higher in the unrecovered group than in the recovered group (13.5 ± 1.7 vs. 12.7 ± 0.7%, p = 0.046). In the multiple logistic regression model, RDW was the only factor associated with recovery from Bell palsy (odds ratio 1.93, 95% confidence interval 1.02-4.65, p = 0.042). Our preliminary study provides the first evidence that the red cell distribution width (RDW) can predict recovery from Bell palsy in patients aged 50 years and less. Further studies are necessary to elucidate the potential pathophysiological mechanisms for our findings. European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 274 5 2303 2306 20170500 10.1007/s00405-016-4445-z 28197706 fumiya/published_papers/23497831 Yuichiro Horibe Tohru Tanigawa Rei Shibata Hiroshi Nonoyama Fumiya Kano Satoshi Yamaguchi Kenta Murotani Takaki Ogawa Hiromi Ueda Efficacy of the red blood cell distribution width for predicting the prognosis of Bell palsy: a pilot study Eur Arch Otorhinolaryngol 32 Suppl 1 1031 1034 20170200 fumiya/published_papers/23497829 FUMIYA KANO KOHKI MATSUBARA MINORU UEDA HIDEHARU HIBI AKIHITO YAMAMOTO Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte Chemoattractant Protein-1 Synergistically Regenerate Transected Rat Peripheral Nerves by Altering Macrophage Polarity STEM CELLS 35 3 641 653 20170100 fumiya/published_papers/23497793 Tohru Tanigawa Fumiya Kano Daisuke Inukai Tessei Kuruma Adult retropharyngeal abscess Elsevier Ltd IDCases 2214-2509 10 2017 130 131 20170000 10.1016/j.idcr.2017.11.001 2-s2.0-85033373567 fumiya/published_papers/23497837 Kohki Matsubara Yoshihiro Matsushita Kiyoshi Sakai Fumiya Kano Megumi Kondo Mariko Noda Noboru Hashimoto Shiro Imagama Naoki Ishiguro Akio Suzumura Minoru Ueda Koichi Furukawa Akihito Yamamoto Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage Polarity JOURNAL OF NEUROSCIENCE 0270-6474 35 6 2452 2464 20150200 10.1523/JNEUROSCI.4088-14.2015 fumiya/published_papers/23497842 Akihito Yamamoto Kiyoshi Sakai Kohki Matsubara Fumiya Kano Minoru Ueda Multifaceted neuro-regenerative activities of human dental pulp stem cells for functional recovery after spinal Neuroscience Research 2014 78 16 20 20140000 fumiya/published_papers/23497839 Akihito Y Kohki M Fumiya K Kiyoshi S Analysis of the Neuroregenerative Activities of Mesenchymal Stem Cells in Functional Recovery After Rat Spinal Cord Injury Spinal cord injury (SCI) involves concurrent, interacting pathological processes, and requires a multifaceted therapeutic strategy. Stem cell-based transplantation holds great promise as such an approach. We have reported that stem cells derived from human dental pulp have remarkable neuroregenerative activity, and that when transplanted into animal models of SCI, these cells promote functional recovery by inhibiting massive SCI-induced apoptosis, preserving neural fibers and myelin, regenerating transected axons, and replacing damaged cells by differentiating into oligodendrocytes. Here, we introduce some details of our experimental procedures, which may serve as a guide for designing experiments to evaluate the therapeutic benefits of various types of stem cells. Animal Models for Stem Cell Therapy, Methods in Molecular Biology 1213 26 321 328 20140000 10.1007/978-1-4939-1453-1_26 25173394 fumiya/published_papers/33941287 Yao LIU Liu Yao Linze XIA 夏 霖? Fumiya KANO 加納 史也 Noboru HASHIMOTO 橋本 登 Yoshizo MATSUKA 松香 芳三 Akihito YAMAMOTO 山本 朗仁 Eiji TANAKA 田中 栄二 Low-intensity pulsed ultrasound ameliorates neuropathic pain induced by partial sciatic nerve ligation via regulating macrophage polarization Journal of Oral Health and Biosciences 34 11 18 20110900 fumiya/misc/48313197 丁程 橋本登 加納史也 山本朗仁 Conditioned medium from deciduous dental pulp stem cells inhibited RANKL-induced osteoclastogenesis 乳歯歯髄幹細胞の培養上清はRANKL誘導破骨細胞形成を阻害した 日本再生医療学会総会(Web) 23rd null null 20240000 fumiya/misc/48313195 夏霖澤 加納史也 橋本登 田中栄二 山本朗仁 M2 Macrophages induced by MSC Alleviate Osteoarthritis via sFRP1-mediated Wnt signal inhibition MSCによって誘導されるM2マクロファージはsFRP1を介したWntシグナル阻害を介して変形性関節症を軽減する 日本再生医療学会総会(Web) 23rd null null 20240000 fumiya/misc/48313193 徐陽 橋本登 加納史也 山本朗仁 Conditioned medium from stem cells of human exfoliated deciduous teeth ameliorates atopic dermatitis ヒト乳歯の幹細胞由来培養上清はアトピー性皮膚炎を改善する 日本再生医療学会総会(Web) 23rd null null 20240000 fumiya/misc/48313213 山本朗仁 橋本登 加納史也 XIA Linze XIA Linze DING Cheng 田中栄二 高橋伸典 Development of joint regeneration drugs using sialic acid recognition lectins シアル酸認識レクチンを用いた関節再生薬の開発 Journal of Oral Biosciences Supplement (Web) 2187-9109 2022 null null 20220000 fumiya/misc/17954048 Akihito Yamamoto Kohki Matsubara Kiyoshi Sakai Fumiya Kano Minoru Ueda Multifaceted Neuro-Regenerative Activities of Human Dental Pulp Stem Cells for Functional Recovery after Spinal Cord Injury. Tissue Engineering and Regenerative Medicine Chapter 6 1 14 20140000 公益社団法人 日本口腔外科学会 第6回若手口腔外科医優秀論文賞 Therapeutic benefits of factors derived from stem cells from human exfoliated deciduous teeth for radiation-induced mouse xerostomia 20241100 公益社団法人 日本口腔外科学会 2022年度口演発表賞(共著) 歯髄幹細胞培養上清の抗酸化効果による放射線性口腔乾燥症の治療メカニズム 20221100 加納史也 歯科基礎医学会 第62回歯科基礎医学会学術大会 モリタ優秀発表賞 20201000 加納史也 日本口腔外科学会 日本口腔外科学会優秀口演発表賞 20171100 加納史也 日本炎症・再生医学会 優秀演題賞 20160700 加納史也 The 11th World Congress on Inflammation Travel Award 20150800 加納史也 日本炎症・再生医学会 優秀演題賞 20150700 加納史也 日本口腔外科学会 日本口腔外科学会第14回ゴールドリボン賞(共同) 20141000