Ducchenne型筋ジストロフィー剖検登録例の統計学的解析

Ducchenne型筋ジストロフィー剖検登録例の統計学的解析

筋ジストロフィー剖検例登録票447例から，デュシェンヌ型筋ジストロフィー(DMD)301例を選び出し，死因，臓器所見などを検討·解析し，全身状態の経時的変化について考察した．DMDの寿命は確実に延長しており，長期生存例は主要臓器の重量正常例が多く，栄養状態や循環動態を良好に保つことが寿命の延長につながるものと考えられた．

Bulletin of School of Medical Sciences, the University of Tokushima

徳島大学医療技術短期大学部紀要


偽血管腔がみられなかった血管腫様悪性線維組織球腫の1例

偽血管腔がみられなかった血管腫様悪性線維組織球腫の1例


筋ジストロフィー剖検登録票によるデュシェンヌ型筋ジストロフィー剖検例の統計学的解析

筋ジストロフィー剖検例登録票422例から，デュシェンヌ型筋ジストロフィー(以下DMD)295例を選び出し，死因，臓器所見などを検討·解析し，全身状態の経時的変化について考察した．DMD症例の平均寿命は1970年代では17.9歳であったが，1990年代後半では24.5歳になり，明らかな延長が認められた．30歳以上生存者は1985年以降の症例であった．死因は肺感染症が著減し，最近は心不全や呼吸不全が大半を占めており，寿命延長の理由として抗生物質や呼吸管理の進歩によることが推測された．主要臓器の重量は，25歳以上の生存例では重量正常例が多かった．栄養状態や循環動態を良好に保つことも，長期生存に関与していることを示唆している．

徳島大学医療技術短期大学部紀要


Phenotypic modulation of smooth muscle cells in human cerebral aneurysmal walls

We used immunohistochemical methods to analyze the phenotypes of smooth muscle cells (SMCs) in human cerebral arteries and aneurysmal walls. Thirty-two aneurysmal walls were studied; 31 aneurysmal walls were resected at operation and 1 aneurysm was obtained at autopsy. Seven control arteries were obtained at autopsy. Semiserial sections were subjected to immunohistochemical staining with antibodies to alpha-smooth muscle actin (alpha-SMA), desmin and smooth muscle myosin heavy chain isoforms: SM1, SM2 and SMemb. In control cerebral arteries, SMCs in the media were strongly immunostained for alpha-SMA, desmin, SM1 and SM2; immunoreactivity for SMemb was faint or weakly positive. SMCs in both non-ruptured and ruptured aneurysmal walls showed no staining for desmin; the expression of alpha-SMA was well preserved. Compared with control cerebral arteries, in 4 of 11 non-ruptured aneurysmal walls, the staining intensity of SMCs for SMemb was clearly increased. In ruptured aneurysmal walls, the expression of SM2 was lower than in control cerebral arteries and non-ruptured aneurysmal walls. Our study suggests that the phenotype of SMCs in aneurysmal walls is different from the contractile type in the media of normal cerebral arteries, at least partially changing to the synthetic type in some non-ruptured aneurysms. SMCs in ruptured aneurysmal walls may have lost both phenotypes before rupture. Phenotypic modulation of SMCs in the aneurysmal walls appears to be related to a remodeling of the aneurysmal wall and to a rupture mechanism.


Duchenne型筋ジストロフィー剖検例における梗塞病変の病理組織学的検討

Duchenne型筋ジストロフィー剖検例における梗塞病変の病理組織学的検討

Duchenne型筋ジストロフィー(DMD)剖検例の梗塞病変について一般剖検例と比較検討した．DMD44例中9例(20.4%)に1臓器以上に梗塞病変を認めた．梗塞合併DMD9例の平均年齢は20.6歳で，平均心重量は407.2±125.2gで非梗塞例の心重量255.5±115.4gと比較し有意に重量増加があった．一般剖検500例のうち54例(10.8%)に1臓器以上の梗塞病変を認めた．梗塞合併例の平均年齢は65.3で，平均心重量は353.0±91.9gであった．DMDでは若年で動脈硬化も認めないが，梗塞病変の頻度が低くないことが明らかとなった．


The utility and limitations of an ultrasonic miniprobe in the staging of gastric cancer

To determine the utility and limitations of an ultrasonic miniprobe (UMP) in the staging of gastric cancer, we evaluated 46 patients who underwent endoscopic ultrasonography (EUS) using an UMP and who were histologically determined to have gastric cancers. In every case, UMP findings were compared with histopathological findings after treatment. The total accuracy of UMP relative to the depth of tumor invasion was 71.7% (33/46 cases). Accuracy with respect to T1-m tumor diagnosis was 75.7% (22/29 cases), and for T1-sm, 76.9% (10/13 cases), but accuracy for T2 tumor diagnosis was low, due to ultrasound attenuation. When the analysis was carried out based on the size of tumor, the accuracy for UMP was 50.0% (9/18 cases) for all tumors over 20 mm and 85.7% (24/28 cases) for all tumors smaller than 20 mm. We conclude that UMP is suitable for investigation of tumor extension when the lesion is superficial and/or small gastric cancers which do not cause ultrasonic attenuation, but not when the tumor is large or located in certain sites, although conventional EUS is useful in some of these cases.

The Journal of Medical Investigation : JMI


Infrequent Detectable Somatic Mutations of the RET and Glial Cell Line-Derived Neurotrophic Factor (GDNF) Genes in Human Pituitary Adenomas.

RET is a receptor tyrosine kinase expressed in neuroendocrine cells and tumors. RET is activated by a ligand complex comprising glial cell line-derived neurotrophic factor (GDNF) and GDNF receptor-alpha (GDNFR-alpha). Activating mutations of the RET proto-oncogene were found in multiple endocrine neoplasia (MEN) 2 and in sporadic medullary thyroid carcinoma and pheochromocytoma of neuroendocrine origin. Mutations of the RET proto-oncogene and the glial cell line-derived neurotrophic factor (GDNF) gene were examined in human pituitary tumors. No mutations of the RET proto-oncogene including the cysteine-rich region or codon 768 and 918 in the tyrosine kinase domain were detected in 172 human pituitary adenomas either by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) or by PCR-restriction fragment length polymorphism (RFLP). Further, somatic mutations of the GDNF gene in 33 human pituitary adenomas were not detected by PCR-SSCP. One polymorphism of the GDNF gene at codon 145 of TGC or TGT was observed in a prolactinoma. The RET proto-oncogene message was detected in a normal human pituitary gland or 4 of 4 human pituitary adenomas with reverse transcription (RT)-PCR, and in rodent pituitary tumor cell lines with Western blotting. The expression of GDNF gene was detected in 1 of 4 human somatotroph adenomas, 1 of 2 corticotroph adenomas, and 2 of 6 rodent pituitary tumor cell lines with RT-PCR. Based on these, it is concluded that somatic mutations of the RET proto-oncogene or the GDNF gene do not appear to play a major role in the pituitary tumorigenesis in examined tumors.


9年間内視鏡的に経過を観察し得た早期胃癌の1例

9年間内視鏡的に経過を観察し得た早期胃癌の1例


An autopsy case of cytokeratin 7-positive minute adenocarcinoma of the lung with systemic metastases

We describe a 60-year-old woman with leg pain. Although metastatic bone tumor and atypical cells mimicking signet-ring cells in the bone marrow picture were observed, systemic survey revealed no primary lesion. The patient died two months after admission from systemic progress of the disease. Autopsy revealed a small focus of adenocarcinoma within the right upper lobe of the lung and systemic metastases without any particular changes in the gastrointestinal tract. The tumor cells of the lung were diffusely positive for cytokeratin 7, whereas cytokeratin 20 immunoreactivity was weak and focal, and that supported the lung origin of the present tumor. Moreover, the tumor cells in the bone marrow showed a similar pattern in immunoreactivity. These findings suggest that cytokeratin 7 and cytokeratin 20 immunoreactivity is helpful for the premortem diagnosis of the metastatic tumor of unknown origin.


Analysis of loss of heterozygosity on chromosome 11 and infrequent inactivation of the MEN1 gene in sporadic pituitary adenomas

To investigate the role of tumor suppressor genes in sporadic pituitary adenomas, we first analyzed loss of heterozygosity on 11q13 with microsatellite analysis in 31 tumors. Loss of heterozygosity on 11q13 was detected in 1 mixed GH/PRL adenoma, and the somatic 22-bp deletion of the multiple endocrine neoplasia type 1 (MEN1) gene encoding menin was detected in this tumor. Trisomy 11 suggested by the decreased mean allelic ratios of 66% or 65% for 16 or 13 microsatellite markers, respectively, in 2 of 31 pituitary adenomas was confirmed by interphase fluorescence in situ hybridization. Screening for mutations of the MEN1 gene did not find mutations with PCR-single strand conformation polymorphism analysis in other pituitary adenomas retaining heterozygosity on 11q13. Based on these, it is concluded that inactivation of the MEN1 gene comprises a rare etiology for tumorigenesis of the pituitary gland, and that trisomy 11 or another gene(s) may contribute to the pathogenesis of sporadic pituitary adenomas.

The Journal of Clinical Endocrinology and Metabolism


Abrogation of Autoimmune Diabetes in Nonobese Diabetic Mice and Preotection against Effector Lymphocytes by Transgenic Paracrine TGFb-1.


Analysis of Loss of Heterozygosity on Chromosome 11 and Infrequent Inactivation of MEN1 Gene in Sporadic Pituitary Adenomas.


Familial Isolated Primary Hyperparathyroidism with Parathyroid Carcinomas: Clinical and Molecular Features.

Familial isolated primary hyperparathyroidism (FIHP) is a rare hereditary disorder. We present four patients from a single family with FIHP, and genetic analysis of their parathyroid adenomas and parathyroid carcinoma. DNA was extracted from tumours resected at surgery. Tumours were examined for loss of heterozygosity (LOH) with microsatellite polymorphic markers. The 27-year-old proband (Patient 1) died of parathyroid carcinoma with metastases to the lungs and chest wall. Sixteen years later, his 34-year-old sister (Patient 2) presented with a neck tumour and primary hyperparathyroidism. Family screening revealed parathyroid tumours in his 36-year-old sister (Patient 3) and 29-year-old cousin (Patient 4). Histological examination of resected tumours showed parathyroid carcinoma and adenoma in Patient 2, a parathyroid adenoma in Patient 3, and an atypical parathyroid adenoma in Patient 4. Autopsy of the proband ruled out multiple endocrine neoplasia (MEN) type 1, and the three patients who underwent parathyroidectomy did not exhibit any abnormalities in the pancreas or the pituitary gland. Analysis of tumour DNA from one parathyroid carcinoma (Patient 2), the atypical parathyroid adenoma (Patient 4), and two parathyroid adenomas (Patients 2 and 3) showed limited LOH on chromosomes 13q12.3-q32 in an adenoma of Patient 2 and 9p21-p22 and 13q12.3-q32 in an adenoma of Patient 3. These results suggest the possible contribution of tumour suppressor genes including the retinoblastoma gene and the hereditary breast cancer susceptibility gene (BRCA2) on 13q to parathyroid tumours in this family.


CD34 expression by Inflammatry fibroid polyp of the stomach

Inflammatory fibrinoid polyp(IFP)は比較邸珍しい非腫瘍性病変で，消化管では胃によく見られる．今回11例12個のIFPの組織学的，免疫組織化学的検討をした．病変は平均1.0cm大の大きさで，全例前庭部に発生していた．7個は粘膜と粘膜下層に存在し，4個は粘膜内に，1個は粘膜下層にのみ存在していた．組織学的に特徴的な紡錘形細胞の増殖が見られたが，それらの細胞は免疫組織化学的にはvimentinとCD34が陽性であった．さらに7個の病変では組織球マーカーが陽性で，3個では平滑筋マーカーが陽性であった．


Purification, characterization and localization of a novel trypsin-like protease found in the human airway

A novel trypsin-like protease was purified to homogeneity from the sputum of patients with chronic airway diseases, by sequential chromatographic procedures. The enzyme migrated on SDS-polyacrylamide gel electrophoresis to a position corresponding to a molecular weight of 28 kDa under both reducing and non-reducing conditions, and showed an apparent molecular weight of 27 kDa by gel filtration, indicating that it exists as a monomer. It had an NH2-terminal sequence of Ile-Leu-Gly-Gly-Thr-Glu-Ala-Glu-Glu-Gly-Ser-Trp-Pro-Trp-Gln-Val-Ser-Leu- Arg-Leu, which differed from that of any known protease. Studies with model peptide substrates showed that the enzyme preferentially cleaves the COOH-terminal side of arginine residues at the P1 position of certain peptides, cleaving Boc-Phe-Ser-Arg-4-methylcoumaryl-7-amide most efficiently and having an optimum pH of 8.6 with this substrate. The enzyme was strongly inhibited by diisopropyl fluorophosphate, leupeptin, antipain, aprotinin, and soybean trypsin inhibitor, but hardly inhibited by secretory leukocyte protease inhibitor at 10 microM. An immunohistochemical study indicated that the enzyme is located in the cells of the submucosal serous glands of the bronchi and trachea. These results suggest that the enzyme is secreted from submucosal serous glands onto the mucous membrane in patients with chronic airway diseases.

American Journal of Respiratory Cell and Molecular Biology


Exercise training in Otsuka Long-Evans Tokushima Fatty rat, a model of spontaneous non-insulin-dependent diabetes mellitus: effects on the B-cell mass, insulin content and fibrosis in the pancreas

The effects of exercise on alterations in the amount of B-cell mass, insulin content and fibrous tissue present in the pancreas were examined for a diabetic state induced by a 70% pancreatectomy and a prediabetic state in Otsuka Long-Evans Tokushima Fatty (OLETF) rat, a model for the spontaneous development of non-insulin-dependent diabetes mellitus (NIDDM). The rats (5-weeks old) were trained either by a 6-week running program or sedentary controls, and at 6-weeks of age, received either a 70% pancreatectomy or a sham-pancreatectomy (sham). As in our previous report, persistent hyperglycemia was detected after surgery for both trained pancreatectomized (Px) and sedentary Px groups. In the nondiabetic sham rats, exercise training resulted in a significantly smaller increase in body weight and beneficial effects on the pancreas as reflected by an increase in pancreatic volume, accompanied by increases in B-cell mass and insulin content as well as less connective tissue in the pancreas compared with the sedentary nondiabetic sham rats. The effect was not sufficient to improve sustained hyperglycemia in the trained diabetic Px rats. This is probably due to a decreased capacity for B-cell proliferation in response to an increased demand for insulin. Although exercise failed to improve this inherent defect in B-cell proliferation, it ameliorated the further deterioration of the pancreas which occurred with hyperglycemia, and resulted in a higher quantity of insulin stored per milligram of B-cell mass (as function of B-cell mass) and less fibrosis in the pancreas, compared with the sedentary diabetic Px rats. The findings of the present study suggest that exercise training has a beneficial effect on the pancreas in the nondiabetic state, and also exerts some positive effects in the diabetic state in this model rat.


Infrequent Mutations of p27Kip1 Gene and Trisomy 12 in a Subset of Human Pituitary Adenomas.


Infrequent Mutations of p16INK4A and p15INK4B Genes in Human Pituitary Adenomas.


Inactivation of the Tumor Suppressor Gene on 11q13 in Brothers with Familial Acrogigantism without Multiple Endocrine Neoplasia Type 1.


Multiple endocrine neoplasms in a patient with AL amyloidosis-associated plasma cell dyscrasia

Reports on patients with systemic amyloidosis-associated plasma cell dyscrasia (PCD) who have multiple endocrine tumors are very rare. Here we describe such a case. The patient was a 74-yr-old man with amyloid light-chain (AL) amyloidosis-associated PCD who had a null-cell adenoma of the pituitary and a latent papillary carcinoma of the thyroid gland. as well as a tubular adenoma of the sigmoid colon. The amyloid protein deposits reacted with the antibody to the n-immunoglobulin light chain. Since PCD by itself may be a risk factor for the development of subsequent neoplasms, the careful clinical evaluation of PCD patients is recommended.


Impaired β-cell Function and Deposition of Fat dropleets in Pancreas as a Consequence of Hypertriglyceridemia in OLETF Rat, a Model of Spontaneous NIDDM.


Prevention of Adoptively Transferred Diabetes in Non-Obese Diabetic Mice with IL-10-Transduced Islet-Specific Th1 Lymphocytes: A Gene Therapy Model for Autoimmune Diabetes.


Obvious mRNA and Protein Expression but Absence of Mutations of the RET Proto-Oncogene in Parathyroid Tumors.


Poor capacity for proliferation of pancreatic β-cells in Otsuka-Long-Evans-Tokushima Fatty Rat: a model of spontaneous NIDDM.


Macrophage colony-stimulating factor-producing malignant histiocytosis


Two Different Pituitary Adenomas in a Patient with Multiple Endocrine Neoplasia Type 1Associated with Growth Hormone-Releasing Hormone-Producing Pancreatic Tumor: Clinical and Genetic Features.

The clinical and genetic features of a 43-year-old male patient with multiple endocrine neoplasia type 1 were reported. He developed hyperparathyroidism, a GHRH-producing pancreatic tumor, and acromegaly between 1980 and 1983. Because his pituitary gland increased in size even after resecting the GHRH-producing pancreatic tumor, transsphenoidal hypophysectomy was performed six years later. The pituitary contained two histologically-different adenomas composed of somatotroph cells and null cells. Genetic analyses revealed loss of heterozygosity on chromosome 11 in common in the pituitary adenomas, the pancreatic endocrine tumors, and a parathyroid hyperplasia. On the other hand, mutations of ras, p53, Gs alpha, and Gi2 alpha genes were not found in these tumors. The loss of the tumor suppressor gene on chromosome 11q12-13 was involved in the formation of two pituitary adenomas, two pancreatic endocrine functioning tumors, and a parathyroid hyperplasia in this patient, but the tumorigenic factors in the specific endocrine organs remain to be studied.


Transgenic Expression of IL-10 in Pancreatic Islet A cells Accelerates Autoimmune Insulitis and Diabetes in Non-Obese Diabetic Mice.


中枢性尿崩症を合併したリンパ球性下垂体炎の1例

中枢性尿崩症を合併したリンパ球性下垂体炎の1例


Analysis of the Gsα gene in a growth hormone-secreting pituitary adenomas by the polymerase chain reaction-direct sequencing method using paraffin-embedded tissues


Demonstration of biological activity of a growth hormone-releasing hormone-like substance produced by a pheochromocytoma


Gsα遺伝子の点突然変異を認めたMENI 型の下垂体腺腫症例

Gsα遺伝子の点突然変異を認めたMENI 型の下垂体腺腫症例

ホルモンと臨床, 冬増刊号(間脳下垂体腫瘍 Ⅱ)

ホルモンと臨床, 冬増刊号(間脳下垂体腫瘍 Ⅱ)


Rare Mutation of Gs Alpha Subunit Gene in Human Endocrine Tumors: Mutation Detection by Polymerase Chain Reaction-Primer-Introduced Restriction Analysis.

The Gs alpha (Gs alpha) gene can be activated to the putative oncogene gsp by specific point mutations at codons 201 or 227. Such mutations have been reported in growth hormone (GH)-secreting pituitary adenomas and thyroid tumors. To clarify the role of Gs alpha gene in human endocrine tumors, 197 tumors were screened for point mutations at codons 201 or 227 of the Gs alpha gene. Mutations were detected by primer-introduced restriction analysis (PIRA) of the polymerase chain reaction (PCR) product of genomic DNA. These Gs alpha mutations were present in 4 of 53 pituitary adenomas (4 of 43 GH-secreting adenomas; 1 of these 4 was a GH- and prolactin-secreting adenoma from a patient with familial multiple endocrine neoplasia Type 1), 4 of 66 thyroid tumors (4 of 30 papillary carcinomas), and 1 of 19 adrenocortical adenomas (1 of 6 aldosterone-secreting adenomas). In contrast, none of these Gs alpha mutations were detected in parathyroid tumors, endocrine pancreatic tumors, or pheochromocytomas. Gs alpha mutations at these two loci may play a role in the pathogenesis of a small population of GH-secreting pituitary adenomas, papillary thyroid carcinomas, and adrenocortical adenomas, but that they are not involved in the pathogenesis of other types of endocrine tumors.


下垂体腺腫を伴ったアミロイドーシスの一症例

下垂体腺腫を伴ったアミロイドーシスの一症例


A pituitary specific point mutation of codon 201 of the Gsα gene in a pituitary adenoma of a patient with multiple endocrine neoplasia (MEN) type 1


Ras Mutations in Endocrine Tumors: Mutation Detection by PCR-SSCP.


Primary adult T-cell lymphoma of the breast


風疹罹患後にIBL型病変を認めた一例

風疹罹患後にIBL型病変を認めた一例

52歳，女性．37℃台の発熱と全身の淡紅色の小斑状疹，有痛性の頸部リンパ節腫張で発症．肝，脾腫も認めた．末梢血に異型リンパ球を認め，抗風疹ウイルス抗体512倍と陽性であった．リンパ節生検でリンパ節基本構造の破壊，樹枝状血管の増生，immunoblasstの増殖がありIBL型病変と診断した．methylprednisoloneのパルス療法とγ-globulin療法により病態は改善した．本例は，ウイルス感染に伴う腫瘍性格の明確でないIBL型病変を，より良性な真のIBLとの関連で検討し，またIBLの発症機序を検討する上に貴重な症例である．

The Japanese Journal of Clinical Hematology


胆嚢摘出後に高カルシウム血症性クリーゼを来たした原発性副甲状腺機能亢進症の1例

胆嚢摘出後に高カルシウム血症性クリーゼを来たした原発性副甲状腺機能亢進症の1例


Paraganglioma of the cauda equina : An ultrastructural and immunohistochemical study of two cases

The ultrastructural and immunohistochemical features of 2 paragangliomas arising in the cauda equina are described. In both cases the tumor cells were arranged in small nests or cords and contained characteristic neurosecretory granules, lamellar stacks of rough endoplasmic reticulum (RER), and some well-developed Golgi apparatuses in their cytoplasm. The cells varied in electron density; the darker cells, occasionally resembling sustentacular cells, were probably dehydrated light cells because they contained a few neurosecretory granules. Sustentacular cells were difficult to identify by electron microscopy, but irregularly distributed S-100 protein and glial fibrillary acidic protein (GFAP) were found in these cells by immunostaining. Many tumor cells contained abundant neurofilaments. Curiously, a few cytokeratin-positive cells were found in 1 case. On microscopic examination, a small area of ganglioneuroma was found associated with the paraganglioma in 1 case. Ganglionic differentiation was concluded to be frequent in paragangliomas of the cauda equina region as in duodenal paragangliomas.

馬尾に発生した傍神経節腫について超微形態的および免疫組織化学的に検討した．2例とも腫瘍細胞は小胞巣状に配列し，細胞内に特徴的な神経分泌顆粒を含み，粗面小胞体·ゴルジ装置が発達していた．十二指腸の傍神経節腫と同様に馬尾の本腫瘍も神経節細胞への分化がしばしば見られることがわかった．


Ectopic Growth Hormone-Releasing Hormone (GHRH) Syndrome in a Case with Multiple Endocrine Neoplasia Type 1.


Production and secretion of immunoreactive growth hormone-releasing factor by pheochromocytomas


Immunoreactive Somatostatin and Calcitonin in Pulmonary Neuroendocrine Tumor

A well-differentiated neuroendocrine carcinoma of the lung that secreted immunoreactive somatostatin (IR-SRIF) and IR-calcitonin (CT) in a 72-year-old women is described. The plasma concentrations of IR-SRIF (57.5 pg/ml) and IR-CT (340 pg/ml) before operation were significantly higher than the respective normal ranges. After resection of the tumor, the plasma CT level (105 pg/ml) decreased to within the normal range, and the SRIF level (32.7 pg/ml) also decreased, but was still abnormally higher, which suggested the presence of an unidentified remnant of the tumor. Abnormal accumulation of technetium 99m (99mTc) in the lumbar vertebrae was found 6 months after the operation, which indicated a metastatic tumor. The tissue concentrations of IR-SRIF and IR-CT were 103 and 94 ng/g wet weight, respectively, and SRIF-IR tumor cells and CT-IR tumor cells were demonstrated immunohistochemically. On gel-filtration chromatography of the tumor tissue, two peaks of SRIF immunoreactivity were eluted in the positions of synthetic SRIF-28 and SRIF-14, respectively. Conversion of SRIF-28 to SRIF-14 was suggested from results on changes in the two IR-SRIF components during incubation with a crude enzyme preparation extracted from the tumor tissue.


Polypoid traumatic neuroma of the gallbladder

胆石のため胆嚢摘出術を行った症例で，摘出胆嚢内にポリープ状の外傷性神経腫を発見した．患者は約20年前，胆嚢摘出術を受けたが，癒着等のため摘出できずに手術を終えた既往があり，本腫瘍はそのときに受けた障害のためと考えられる．本例は胆嚢内に発見された最初の報告例である．

Archives of Pathology and Laboratory Medicine


Production of growth hormone-releasing factor in pheochromocytoma


Co-storage and co-secretion of somatostatin and catecholamine in bovine adrenal medulla


Immunohistochemistry of myoglobin in rhabdomyosarcomas

ミオグロビン免疫組織化学的染色の病理診断における有用性をヒト骨格筋と横紋筋肉腫で検討した．ミオグロビン陽性細胞はヒト正常骨格筋と，横紋筋肉腫26例のうちの13例に認められた．ミオグロビン陽性の横紋筋肉腫13例中，横紋が認められたのは6例で，他の7例には認められなかった．また2例では横紋が認められたにもかかわらずミオグロビンは証明されなかった．ミオグロビン免疫組織化学は横紋筋肉腫の診断を確定するのに役立つと思われた．


Distribution and characteristics of immunoreactive somatostatin, vasoactive intestinal polypeptide and calcitonin in tumors


Fetal and maternal plasma levels of immunoreactive somatostatin at delivery: evidence for its increase in the umbilical artery and its arterio-venous gradient in the feto-placental circulation

The concentrations of immunoreactive somatostatin (IR-SRIF) in plasma samples taken from the umbilical artery (UA), umbilical vein (UV), and maternal antecubital vein (MV) were measured in 23 cases of normal delivery at term. High concentrations of IR-SRIF were detected in the plasma from the UA (mean +/- SD, 73.2 +/- 40.6 pg/ml), the value being about 2.5 times that in the UV (29.5 +/- 17.5 pg/ml; P less than 0.001). An arterio-venous gradient was observed in all 23 subjects. The plasma level of IR-SRIF in the MV (10.9 +/- 4.6 pg/ml) was significantly lower than those in the UA (P less than 0.001) and UV (P less than 0.001) and was almost the same as that in nonpregnant women (12.7 +/- 5.4 pg/ml). Chromatographic analysis of an extract of plasma from the UA gave only one peak of immunoreactive material, which was eluted in the same position as synthetic SRIF-14. A similar result was obtained in nonpregnant women. No correlation was found between the plasma level of IR-SRIF and that of GH, insulin, glucagon, or gastrin in the UA. The present findings suggest that the high concentration of IR-SRIF in the feto-placental circulation originates from the fetus and reflects a particular role of this peptide in the process of functional maturation of the neuroendocrine system in early human life.

The Journal of Clinical Endocrinology and Metabolism


1. 十二指腸悪性リンパ腫と早期胃癌を合併した1例

1. 十二指腸悪性リンパ腫と早期胃癌を合併した1例


Immunoreactive somatostatin in catecholamin-producing extra-adrenal paraganglioma

副腎外の傍神経節腫患者の血清で，ソマトスタチン免疫活性が高値を示した．摘出腫瘍組織中に多量のソマトスタチンと免疫組織化学的方法でソマトスタチン検疫細胞を認めた．腫瘍抽出物のクロマトグラフィー分析で2種類のソマトスタチン免疫活性(すなわちSRIF14とSRIF28)が示された．本例は分子量の異なるソマトスタチンを産生する副腎外傍神経節腫の最初の報告である．


Somatostatin-immunoreactive cells in human retina and retinoblastoma

ヒト正常網膜および網膜芽細胞腫で，ソマトスタチン免疫活性細胞の局在を免疫組織化学的に検討した．ソマトスタチン陽性細胞は，正常網膜では内顆粒層と神経細胞層に存在し，網膜芽細胞腫では腫瘍組織内に散在しており，輪状の胞体を有しており，ときには短い突起を有していた．網膜芽細胞腫は神経ペプチド産生能力を有する網膜細胞に由来するというはじめての報告である．


Demonstration of somatostatin production in medullary carcinoma of the thyroid

7例の甲状腺髄様癌で抗ソマトスタチン特異血清を用いてソマトスタチンの局在と放出を調べた．4例で血中ソマトスタチン値とカルシトニン値が，3例で腫瘍組織内ソマトスタチン値が高値を示した．免疫組織化学的には腫瘍細胞の大部分はソマトスタチンおよびカルシトニン免疫活性細胞から成っていた．甲状腺髄様癌はソマトスタチン産生腫瘍の1つであることが示唆された．


【内分泌病理学最近の進歩2002】副腎髄質と傍神経節から同時発生した小児褐色細胞腫