6339428/published_papers/54127285 Takashi Uebanso Kei Kobayashi Ayumi Masuda Hitomi Iba Mutsumi Aihara Takaaki Shimohata Kazuaki Mawatari Akira Takahashi Targeted Regulation of Protein Expression in Vibrio parahaemolyticus. V. parahaemolyticus has several virulence factors, including thermostable direct hemolysin (TDH), TDH-related hemolysin (TRH), and two separate type III secretion systems (T3SSs), T3SS1 and T3SS2. T3SS1 is responsible for cytotoxicity, primarily through the activity of its effector VP1680. To gain a detailed understanding of the relationship between the amount of effector, its expression timing, and cytotoxicity, a system is required to regulate protein expression levels and timing. In the present study, we developed an effector protein expression system controlled by an arabinose-dependent transcription factor and found that cytotoxicity toward mammalian cells increased in a VP1680-dependent manner. To ensure specific protein degradation, we also established a targeted protein degradation system, including VP0917 (ClpP) and VP0918 (ClpX)-, or VP0917 and VP1014 (ClpA)-mediated degradation of ssrA-tagged proteins (proteins bearing the C-terminal degradation tag encoded by tmRNA). By combining these systems, more than 50% of the targeted protein could be degraded within 20 min. As a byproduct of creating the systems, we obtained an enhanced green fluorescent protein variant that emits strong fluorescence in V. parahaemolyticus. The protein degradation system developed in this study has demonstrated the potential to control intracellular protein levels to a certain extent. Moreover, experimentally controlling intracellular protein levels will allow for a more detailed examination of the relationship between protein quantity and cellular phenotype, potentially overcoming the limitations of the "all-or-nothing" model. Biology 15 5 null null 20260305 10.3390/biology15050430 41823855 6339428/published_papers/52797943 Kazuaki Mawatari Yushi Onoda Yasuko Kadomura-Ishikawa Takahiro Emoto Momoka Yamaguchi Nozomi Hirano Sae Toda Mina Matsubara Takashi Uebanso Toshihiko Aizawa Shigeharu Yamauchi Yasuo Fujikawa Tomotake Tanaka Xing Li Eduardo Suarez-Lopez Richard J Kuhn Ernest R Blatchley 3rd Akira Takahashi Evaluation and Comparison of the UV-LED Action Spectra for Photochemical Disinfection of Coliphages and Human Pathogenic Viruses. Ultraviolet (UV) disinfection is a powerful method for inactivating viruses. However, comparative wavelength-dependent sensitivities among human viruses and bacteriophages remain poorly characterized. Here, we evaluated the virucidal efficiencies of UV-light emitting diode (UV-LED) against multiple coliphages (MS2, Qβ, PhiX174, and T1) and mammalian viruses, including respiratory syncytial virus (RSV) and human metapneumovirus (HMPV). We used a standardized irradiation system equipped with interchangeable UV-LED modules (250-365 nm), a low-pressure mercury lamp (254 nm), and a filtered krypton-chloride excimer lamp (222 nm). All coliphages exhibited wavelength-dependent inactivation with maximal efficiency at 263-270 nm, closely matching the action spectra of RSV and HMPV (r > 0.94, p < 0.001). However, their absolute UV sensitivities were markedly lower: under 254-281 nm irradiation. RSV and HMPV were approximately 21 and 12 times more sensitive than MS2, respectively. In contrast, far-UVC (222 nm) irradiation reduced these differences, indicating simultaneous damage to viral genomes and structural proteins. These results demonstrated that coliphages and human viruses exhibit similar wavelength-dependent sensitivity to UV-LED irradiation but differ in their absolute susceptibility. Therefore, while coliphages can be conservative surrogates for evaluating UV-LED virucidal performance, their applicability to far-UVC assessments should be interpreted with caution. Microorganisms 13 12 null null 20251209 10.3390/microorganisms13122798 41472001 6339428/published_papers/54127307 北川 若奈 馬渡 一諭 上番増 喬 松原 未奈 長橋 美晴 越山 達貴 鈴村 啓 前田 祥太郎 高橋 章 紫外線照射による空調機内部の真菌増殖抑制と作用機構の検討 (一社)室内環境学会 室内環境学会学術大会講演要旨集 2025 314 315 20251200 6339428/published_papers/54127305 牧本 真奈 下畑 隆明 福島 志帆 山中 咲季 上番増 喬 馬渡 一諭 高橋 章 Campylobacter jejuniの病原性に対するコハク酸代謝の影響 日本カンピロバクター研究会 日本カンピロバクター研究会総会抄録集 18 13 13 20251200 6339428/published_papers/54127302 小野 実優 牧本 真奈 下畑 隆明 上番増 喬 馬渡 一諭 高橋 章 胆汁酸刺激に対するCampylobacter jejuniの応答解析 日本カンピロバクター研究会 日本カンピロバクター研究会総会抄録集 18 15 15 20251200 6339428/published_papers/54127299 下畑 隆明 福島 志帆 木戸 純子 牧本 真奈 上番増 喬 馬渡 一諭 高橋 章 Campylobacter jejuniは宿主アミノ酸トランスポーターを活性化し細胞内で生存する (公社)日本生化学会 日本生化学会大会プログラム・講演要旨集 98 2P 387 20251100 6339428/published_papers/54127296 牧本 真奈 下畑 隆明 福島 志帆 山中 咲季 上番増 喬 馬渡 一諭 高橋 章 Campylobacter jejuniのアミノ酸・コハク酸代謝による病原性変化 (公社)日本生化学会 日本生化学会大会プログラム・講演要旨集 98 3P 403 20251100 6339428/published_papers/52536836 Kazuaki Mawatari Yasuko Kadomura-Ishikawa Takahiro Emoto Yushi Onoda Kai Ishida Sae Toda Takashi Uebanso Toshihiko Aizawa Shigeharu Yamauchi Yasuo Fujikawa Tomotake Tanaka Xing Li Eduardo Suarez-Lopez Richard J. Kuhn Ernest R. Blatchley III Akira Takahashi Viral Inactivation by Light-Emitting Diodes: Action Spectra Reveal Genomic Damage as the Primary Mechanism Irradiation with ultraviolet light-emitting diodes (UV-LEDs) represents a promising method for viral inactivation, but a detailed understanding of the wavelength-dependent action spectra remains limited, particularly across different viral components. In this study, we established standardized UV action spectra for infectivity reduction in pathogenic viruses using a system equipped with interchangeable LEDs at 13 different peak wavelengths (250–365 nm). The reduction in viral infectivity induced by UV-LED exposure was strongly related to viral genome damage, whereas no significant degradation of viral structural proteins was detected. Peak virucidal efficiency was observed at 267–270 nm across all tested viruses, representing a slight shift from the traditionally expected 260 nm nucleic acid absorption peak. Enveloped RNA viruses, including influenza A virus, respiratory syncytial virus, and coronavirus, exhibited greater UV sensitivity than nonenveloped viruses such as feline calicivirus and adenovirus. These observations indicate that structural characteristics, such as the presence of an envelope and genome organization, influence UV susceptibility. The wavelength-specific action spectra established in this study provide critical data for optimizing UV-LED disinfection systems to achieve efficient viral inactivation while minimizing energy consumption in healthcare, food safety, and environmental sanitation. MDPI AG Viruses 1999-4915 17 8 1065 1065 20250730 10.3390/v17081065 6339428/published_papers/52392577 Kai Ishida Mina Matsubara Miharu Nagahashi Yushi Onoda Toshihiko Aizawa Shigeharu Yamauchi Yasuo Fujikawa Tomotake Tanaka Yasuko Kadomura-Ishikawa Takashi Uebanso Masatake Akutagawa Kazuaki Mawatari Akira Takahashi Efficacy of ultraviolet-light emitting diodes in bacterial inactivation and DNA damage via sensitivity evaluation using multiple wavelengths and bacterial strains Springer Science and Business Media LLC Archives of Microbiology 1432-072X 207 6 null null 20250425 10.1007/s00203-025-04324-0 6339428/published_papers/54127321 松岡 実花 井上 詩央里 武野 香澄 増田 瑠見子 上番増 喬 馬渡 一諭 高橋 章 ケトジェニック食摂取時の血糖値維持機構の解析 (公社)日本栄養・食糧学会 日本栄養・食糧学会大会講演要旨集 79 203 203 20250400 6339428/published_papers/54127316 Campylobacter jejuniのアミノ酸代謝およびスクシネート代謝が病原性に及ぼす影響(The effect of amino acid and succinate metabolism on Campylobacter jejuni virulence) 日本細菌学会 日本細菌学雑誌 1882-4110 80 2 74 74 20250400 6339428/published_papers/54127313 カンピロバクター・ジェジュニは宿主のアミノ酸トランスポーターを活性化し細胞内生存を促進する(Campylobacter jejuni activates host amino acid transporters to enhance intracellular survival) 日本細菌学会 日本細菌学雑誌 1882-4110 80 2 115 115 20250400 6339428/published_papers/54127310 カンピロバクター・ジェジュニの胆汁刺激に対する応答解析(Analysis of Campylobacter jejuni response to bile stimulation) 日本細菌学会 日本細菌学雑誌 1882-4110 80 2 145 145 20250400 6339428/published_papers/55199856 Koichi Shinoda Kazuaki Mawatari Thi Kim Ngan Bui Yasuko Kadomura-Ishikawa Takashi Uebanso Hitoshi Hirakawa Kenji Awamoto Masayuki Wakitani Tsutae Shinoda Akira Takahashi Development of Mercury-Free Far-UVC Light Source Using Luminous Array Film Technology and Its Germicidal Effects Institute of Electrical and Electronics Engineers (IEEE) IEEE Access 2169-3536 13 80612 80620 20250000 10.1109/access.2025.3561147 6339428/published_papers/52709081 Michiyo Yamashita Mutsumi Aihara Yushi Onoda Shiho Fukushima Miharu Nagahashi Toshihiko Aizawa Shigeharu Yamauchi Yasuo Fujikawa Tomotake Tanaka Yasuko Kadomura-Ishikawa Kai Ishida Mina Matsubara Takashi Uebanso Kazuaki Mawatari Akira Takahashi Wavelength-dependent differences in survival of Cladosporium sphaerospermum exposed to ultraviolet light. Fungi are one of the main sources of microbial contamination in food and residential environments. There has been reported that fungi are more resistant to drugs than are viruses and bacteria, so efficient sterilization methods are still required. In this study, we examined the effectiveness of ultraviolet (UV) sterilization against mold by focusing on the wavelength and radiant flux dependence of UV. UVC or UVA irradiation inactivated the mold species Cladosporium sphaerospermum in a dose-dependent manner. The energy required to obtain the same inactivation differed significantly between UVC irradiation and UVA irradiation. UVC irradiation induced the formation of substantial amounts of cyclobutane pyrimidine dimers, whereas UVA irradiation did not show any significant difference from the unirradiated group. Furthermore, UVC irradiation induced morphological changes that could be observed by electron microscopy. On the other hand, UVA irradiation suppressed the growth rate. These results suggest that UVC and UVA irradiation have different effects on fungi. The results suggest that both UVC and UVA irradiation may help control fungal contamination. Since the effect of UV differs depending on the wavelength of irradiation, it will be necessary to understand the wavelength-specific effect and use it to control fungal contamination in the living environment. J. Med. Invest. 72 : 260-265, August, 2025. The journal of medical investigation : JMI 72 3.4 260 265 20250000 10.2152/jmi.72.260 41183924 6339428/published_papers/54141249 牧本 真奈 下畑 隆明 福島 志帆 山中 咲季 上番増 喬 馬渡 一諭 高橋 章 コハク酸によるCampylobacter jejuni病原性への影響 腸管出血性大腸菌感染症研究会・日本カンピロバクター研究会 腸管出血性大腸菌感染症研究会・日本カンピロバクター研究会プログラム・抄録集 26·17 27 27 20241100 6339428/published_papers/54141246 下畑 隆明 福島 志帆 木戸 純子 牧本 真奈 上番増 喬 馬渡 一諭 高橋 章 Campylobacter jejuniは宿主アミノ酸トランスポーターを活性化し細胞内生存率を高める 腸管出血性大腸菌感染症研究会・日本カンピロバクター研究会 腸管出血性大腸菌感染症研究会・日本カンピロバクター研究会プログラム・抄録集 26·17 35 35 20241100 6339428/published_papers/54127324 上番増 喬 福井 萌加 内藤 千里 下畑 隆明 馬渡 一諭 高橋 章 腸管上皮細胞におけるケトン体分泌調節因子の探索 (公社)日本生化学会 日本生化学会大会プログラム・講演要旨集 97 [1P 122] 20241100 6339428/published_papers/54127334 畑山 翔 下畑 隆明 木戸 純子 天宅 あや 福島 志帆 内藤 有紀 森 加奈子 中本 晶子 粟飯原 睦美 首藤 恵泉 上番増 喬 馬渡 一諭 榎本 崇宏 芥川 正武 酒井 徹 高橋 章 UVA-LEDを用いた調理器具におけるCampylobacter jejuniの殺菌システムに関する検討 日本食品微生物学会 日本食品微生物学会雑誌 1882-5982 41 3 113 118 20240900 6339428/published_papers/54127328 LEDを用いた細菌の紫外線感受性の標準的な評価方法(A standardized evaluation method for bacterial UV sensitivity using light-emitting diodes) 日本細菌学会 日本細菌学雑誌 1882-4110 79 2 93 93 20240600 6339428/published_papers/54141273 関本 大介 内藤 有紀 村井 美岬 上番増 喬 下畑 隆明 馬渡 一諭 高橋 章 下部消化管における鉄の恒常性維持への腸内細菌叢の関連 (公社)日本栄養・食糧学会 日本栄養・食糧学会大会講演要旨集 78 180 180 20240400 6339428/published_papers/54141270 馬渡 一諭 戸田 沙慧 殿脇 壱成 平野 希美 山口 ももか 上番増 喬 高橋 章 敷島 康普 三浦 宏之 新居 佳孝 Yoo Seung-Hee Chen Zheng スダチ特有ポリメトキシフラボン類の概日リズム調整活性の比較と作用メカニズムの解明 (公社)日本栄養・食糧学会 日本栄養・食糧学会大会講演要旨集 78 251 251 20240400 6339428/published_papers/46294168 Kai Ishida Yushi Onoda Yasuko Kadomura-Ishikawa Miharu Nagahashi Michiyo Yamashita Shiho Fukushima Toshihiko Aizawa Shigeharu Yamauchi Yasuo Fujikawa Tomotake Tanaka Takashi Uebanso Masatake Akutagawa Kazuaki Mawatari Akira Takahashi Development of a standard evaluation method for microbial UV sensitivity using light-emitting diodes. Ultraviolet (UV) light is an effective disinfection method. In particular, UV light-emitting diodes (UV-LEDs) are expected to have many applications as light sources owing to their compact form factor and wide range of choices of wavelengths. However, the UV sensitivity of microorganisms for each UV wavelength has not been evaluated comprehensively because standard experimental conditions based on LED characteristics have not been established. Therefore, it is necessary to establish a standard evaluation method based on LED characteristics. Here, we developed a new UV-LED device based on strictly controlled irradiation conditions using LEDs for each wavelength (250-365 nm), checked the validity of the device characteristics and evaluated the UV sensitivity of Escherichia coli using this new evaluation method. For this new device, we considered accurate irradiance, accurate spectra, irradiance uniformity, accurate dose, beam angle, surrounding material reflections, and sample condition. From our results, the following UV irradiation conditions were established as standard: 1 mW/cm2 irradiance, bacterial solution with absorbance value of A600 = 0.5 diluted 10 times solution, solution volume of 1 mL, working distance (WD) of 100 mm. In order to compare the effects of irradiation under uniform conditions on inactivation of microorganisms, we assessed inactivation effect of E. coli by LED irradiation at each wavelength using the U280 LED as a standard wavelength. The inactivation effect for U280 LED irradiation was -0.95 ± 0.21 log at a dose of 4 mJ/cm2. Under this condition of dose, our results showed a high wavelength dependence of the inactivation effect at each UV wavelength peaking at 267 nm. Our study showed that this irradiation system was validated for the standard UV irradiation system and could be contributed to the establishment of food and water hygiene control methods and the development of equipment for the prevention of infectious diseases. Heliyon 10 6 e27456 null 20240330 10.1016/j.heliyon.2024.e27456 38509990 6339428/published_papers/54141276 馬渡 一諭 上番増 喬 下畑 隆明 高橋 章 野原 一成 Yoo Seung-Hee Chen Zheng 小池 宣也 八木田 和弘 敷島 康普 三浦 宏之 新居 佳孝 スダチ果皮特有のフラボノイド・スダチチンの概日リズム調節作用と肝脂質代謝改善作用 徳島医学会 四国医学雑誌 2758-3279 79 5-6 280 280 20240300 6339428/published_papers/47952275 Yushi Onoda Miharu Nagahashi Michiyo Yamashita Shiho Fukushima Toshihiko Aizawa Shigeharu Yamauchi Yasuo Fujikawa Tomotake Tanaka Yasuko Kadomura-Ishikawa Kai Ishida Takashi Uebanso Kazuaki Mawatari Ernest R. Blatchley Akira Takahashi Accumulated melanin in molds provides wavelength-dependent UV tolerance Photochemical and Photobiological Sciences 1474-9092 null null 20240000 10.1007/s43630-024-00632-4 2-s2.0-85204249197 6339428/published_papers/47462784 Junko Kido Takaaki Shimohata Mutsumi Aihara Akari Tsunedomi Sho Hatayama Sachie Amano Yuri Sato Shiho Fukushima Yuna Kanda Aya Tentaku Kai Ishida Hitomi Iba Yumi Harada Takashi Uebanso Kazuaki Mawatari Masatake Akutagawa Akira Takahashi Reduction of Campylobacter jejuni contamination by using UVA-LED and sodium hypochlorite on the surface of chicken meat. Campylobacter jejuni causes gastroenteritis in humans and is a major concern in food safety. Commercially prepared chicken meats are frequently contaminated with C. jejuni, which is closely associated with the diffusion of intestinal contents in poultry processing plants. Sodium hypochlorite (NaClO) is commonly used during chicken processing to prevent food poisoning; however, its antimicrobial activity is not effective in the organic-rich solutions. In this study, we investigated the potential of a new photo-disinfection system, UVA-LED, for the disinfection of C. jejuni-contaminated chicken surfaces. The data indicated that UVA irradiation significantly killed C. jejuni and that its killing ability was significantly facilitated in NaClO-treated chickens. Effective inactivation of C. jejuni was achieved using a combination of UVA and NaClO, even in the organic-rich condition. The results of this study show that synergistic disinfection using a combination of UVA and NaClO has potential beneficial effects in chicken processing systems. Journal of microorganism control 29 2 91 97 20240000 10.4265/jmc.29.2_91 38880621 6339428/published_papers/54141292 小野 実優 石田 快 牧本 真奈 下畑 隆明 上番増 喬 粟飯原 睦美 芥川 正武 榎本 崇宏 馬渡 一諭 岩田 剛敏 高橋 章 UVA照射によるCampylobacter jejuniの上皮定着性に対する影響 日本カンピロバクター研究会 日本カンピロバクター研究会総会抄録集 16 32 32 20231200 6339428/published_papers/45596349 Takashi Uebanso Moeka Fukui Chisato Naito Takaaki Shimohata Kazuaki Mawatari Akira Takahashi SLC16a6, mTORC1, and Autophagy Regulate Ketone Body Excretion in the Intestinal Cells. Ketone bodies serve several functions in the intestinal epithelium, such as stem cell maintenance, cell proliferation and differentiation, and cancer growth. Nevertheless, there is limited understanding of the mechanisms governing the regulation of intestinal ketone body concentration. In this study, we elucidated the factors responsible for ketone body production and excretion using shRNA-mediated or pharmacological inhibition of specific genes or functions in the intestinal cells. We revealed that a fasting-mimicked culture medium, which excluded glucose, pyruvate, and glutamine, augmented ketone body production and excretion in the Caco2 and HT29 colorectal cells. This effect was attenuated by glucose or glutamine supplementation. On the other hand, the inhibition of the mammalian target of rapamycin complex1 (mTORC1) recovered a fraction of the excreted ketone bodies. In addition, the pharmacological or shbeclin1-mediated inhibition of autophagy suppressed ketone body excretion. The knockdown of basigin, a transmembrane protein responsible for targeting monocarboxylate transporters (MCTs), such as MCT1 and MCT4, suppressed lactic acid and pyruvic acid excretion but increased ketone body excretion. Finally, we found that MCT7 (SLC16a6) knockdown suppressed ketone body excretion. Our findings indicate that the mTORC1-autophagy axis and MCT7 are potential targets to regulate ketone body excretion from the intestinal epithelium. Biology 12 12 null null 20231126 10.3390/biology12121467 38132294 6339428/published_papers/43355391 Kazuaki Mawatari Nobuya Koike Kazunari Nohara Marvin Wirianto Takashi Uebanso Takaaki Shimohata Yasuhiro Shikishima Hiroyuki Miura Yoshitaka Nii Mark J Burish Kazuhiro Yagita Akira Takahashi Seung-Hee Yoo Zheng Chen The Polymethoxyflavone Sudachitin Modulates the Circadian Clock and Improves Liver Physiology. SCOPE: Polymethoxylated flavones (PMFs) are a group of natural compounds known to display a wide array of beneficial effects to promote physiological fitness. Recent studies reveal circadian clocks as an important cellular mechanism mediating preventive efficacy of the major PMF Nobiletin against metabolic disorders. Sudachitin is a PMF enriched in Citrus sudachi, and its functions and mechanism of action are poorly understood. METHODS AND RESULTS: Using circadian reporter cells, it shows that Sudachitin modulates circadian amplitude and period of Bmal1 promoter-driven reporter rhythms, and real-time qPCR analysis shows that Sudachitin alters expression of core clock genes, notably Bmal1, at both transcript and protein levels. Mass-spec analysis reveals systemic exposure in vivo. In mice fed with high-fat diet with or without Sudachitin, it observes increased nighttime activity and daytime sleep, accompanied by significant metabolic improvements in a circadian time-dependent manner, including respiratory quotient, blood lipid and glucose profiles, and liver physiology. Focusing on liver, RNA-sequencing and metabolomic analyses reveal prevalent diurnal alteration in both gene expression and metabolite accumulation. CONCLUSION: This study elucidates Sudachitin as a new clock-modulating PMF with beneficial effects to improve diurnal metabolic homeostasis and liver physiology, suggesting the circadian clock as a fundamental mechanism to safeguard physiological well-being. Molecular nutrition & food research 67 9 e2200270 null 20230500 10.1002/mnfr.202200270 36829302 6339428/published_papers/41813055 Kai Ishida Takaaki Shimohata Yuna Kanda Anh Quoc Nguyen Rumiko Masuda Kohei Yamazaki Takashi Uebanso Kazuaki Mawatari Takashige Kashimoto Akira Takahashi Characteristic Metabolic Changes in Skeletal Muscle Due to Vibrio vulnificus Infection in a Wound Infection Model. V. vulnificus causes necrotizing skin and soft tissue infections (NSSTIs) in severe cases, with high mortality and sign of rapid deterioration. Despite the severity of the infection, the dysfunction of the host metabolism in skeletal muscle triggered by V. vulnificus is poorly understood. American Society for Microbiology mSystems 2379-5077 8 2 e0068222 null 20230427 10.1128/msystems.00682-22 36939368 6339428/published_papers/43355388 Shinta Aizawa Takashi Uebanso Takaaki Shimohata Kazuaki Mawatari Akira Takahashi Effects of the loss of maternal gut microbiota before pregnancy on gut microbiota, food allergy susceptibility, and epigenetic modification on subsequent generations. Maternal environments affect the health of offspring in later life. Changes in epigenetic modifications may partially explain this phenomenon. The gut microbiota is a critical environmental factor that influences epigenetic modifications of host immune cells and the development of food allergies. However, whether changes in the maternal gut microbiota affect the development of food allergies and related epigenetic modifications in subsequent generations remains unclear. Here, we investigated the effects of antibiotic treatment before pregnancy on the development of the gut microbiota, food allergies, and epigenetic modifications in F1 and F2 mice. We found that pre-conception antibiotic treatment affected the gut microbiota composition in F1 but not F2 offspring. F1 mice born to antibiotic-treated mothers had a lower proportion of butyric acid-producing bacteria and, consequently, a lower butyric acid concentration in their cecal contents. The methylation level in the DNA of intestinal lamina propria lymphocytes, food allergy susceptibility, and production of antigen-specific IgE in the F1 and F2 mice were not different between those born to control and antibiotic-treated mothers. In addition, F1 mice born to antibiotic-treated mothers showed increased fecal excretion related to the stress response in a novel environment. These results suggest that the maternal gut microbiota is effectively passed onto F1 offspring but has little effect on food allergy susceptibility or DNA methylation levels in offspring. Bioscience of microbiota, food and health 42 3 203 212 20230000 10.12938/bmfh.2022-093 37404565 6339428/published_papers/42302341 Kazuya Tanifuji Yuji Shiozaki Megumi Koike Minori Uga Aoi Komiya Mizuki Miura Ayami Higashi Takaaki Shimohata Akira Takahashi Noriko Ishizuka Hisayoshi Hayashi Yasuhiro Ichida Shuichi Ohtomo Naoshi Horiba Ken-Ichi Miyamoto Hiroko Segawa Effects of EOS789, a novel pan-phosphate transporter inhibitor, on phosphate metabolism : Comparison with a conventional phosphate binder. BACKGROUND: Inorganic phosphate (Pi) binders are the only pharmacologic treatment approved for hyperphosphatemia. However, Pi binders induce the expression of intestinal Pi transporters and have limited effects on the inhibition of Pi transport. EOS789, a novel pan-Pi transporter inhibitor, reportedly has potent efficacy in treating hyperphosphatemia. We investigated the properties of EOS789 with comparison to a conventional Pi binder. METHODS: Protein and mRNA expression levels of Pi transporters were measured in intestinal and kidney tissues from male Wistar rats fed diets supplemented with EOS789 or lanthanum carbonate (LC). 32Pi permeability was measured in intestinal tissues from normal rats using a chamber. RESULTS: Increased protein levels of NaPi-2b, an intestinal Pi transporter, and luminal Pi removal were observed in rats treated with LC but not in rats treated with EOS789. EOS789 but not LC suppressed intestinal protein levels of the Pi transporter Pit-1 and sodium/hydrogen exchanger isoform 3. 32Pi flux experiments using small intestine tissues from rats demonstrated that EOS789 may affect transcellular Pi transport in addition to paracellular Pi transport. CONCLUSION: EOS789 has differing regulatory effects on Pi metabolism compared to LC. The properties of EOS789 may compensate for the limitations of LC therapy. The combined or selective use of EOS789 and conventional Pi binders may allow tighter control of hyperphosphatemia. J. Med. Invest. 70 : 260-270, February, 2023. The journal of medical investigation : JMI 70 1.2 260 270 20230000 10.2152/jmi.70.260 37164731 6339428/published_papers/54141284 福島 志帆 下畑 隆明 牧本 真奈 上番増 喬 馬渡 一諭 高橋 章 Campylobacter jejuniの細胞内生存過程に対するリソソームの関与 日本カンピロバクター研究会 日本カンピロバクター研究会総会抄録集 15 30 30 20221000 6339428/published_papers/43062487 Tomoki Ozaki Yuta Yoshino Ayumi Tachibana Hideaki Shimizu Takaaki Mori Tomohiko Nakayama Kazuaki Mawatari Shusuke Numata Jun-Ichi Iga Akira Takahashi Tetsuro Ohmori Shu-Ichi Ueno Metabolomic alterations in the blood plasma of older adults with mild cognitive impairment and Alzheimer's disease (from the Nakayama Study). Alzheimer's disease (AD) is a progressive disease, and the number of AD patients is increasing every year as the population ages. One of the pathophysiological mechanisms of AD is thought to be the effect of metabolomic abnormalities. There have been several studies of metabolomic abnormalities of AD, and new biomarkers are being investigated. Metabolomic studies have been attracting attention, and the aim of this study was to identify metabolomic biomarkers associated with AD and mild cognitive impairment (MCI). Of the 927 participants in the Nakayama Study conducted in Iyo City, Ehime Prefecture, 106 were selected for this study as Control (n = 40), MCI (n = 26), and AD (n = 40) groups, matched by age and sex. Metabolomic comparisons were made across the three groups. Then, correlations between metabolites and clinical symptoms were examined. The blood mRNA levels of the ornithine metabolic enzymes were also measured. Of the plasma metabolites, significant differences were found in ornithine, uracil, and lysine. Ornithine was significantly decreased in the AD group compared to the Control and MCI groups (Control vs. AD: 97.2 vs. 77.4; P = 0.01, MCI vs. AD: 92.5 vs. 77.4; P = 0.02). Uracil and lysine were also significantly decreased in the AD group compared to the Control group (uracil, Control vs. AD: 272 vs. 235; P = 0.04, lysine, Control vs. AD: 208 vs. 176; P = 0.03). In the total sample, the MMSE score was significantly correlated with lysine, ornithine, thymine, and uracil. The Barthel index score was significantly correlated with lysine. The instrumental activities of daily living (IADL) score were significantly correlated with lysine, betaine, creatine, and thymine. In the ornithine metabolism pathway, the spermine synthase mRNA level was significantly decreased in AD. Ornithine was decreased, and mRNA expressions related to its metabolism were changed in the AD group compared to the Control and MCI groups, suggesting an association between abnormal ornithine metabolism and AD. Increased betaine and decreased methionine may also have the potential to serve as markers of higher IADL in elderly persons. Plasma metabolites may be useful for predicting the progression of AD. Scientific reports 12 1 15205 15205 20220908 10.1038/s41598-022-19670-y 36075959 6339428/published_papers/43062488 Toshiharu Kamishikiryo Go Okada Eri Itai Yoshikazu Masuda Satoshi Yokoyama Masahiro Takamura Manabu Fuchikami Atsuo Yoshino Kazuaki Mawatari Shusuke Numata Akira Takahashi Tetsuro Ohmori Yasumasa Okamoto Left DLPFC activity is associated with plasma kynurenine levels and can predict treatment response to escitalopram in major depressive disorder. AIM: To establish treatment response biomarkers that reflect the pathophysiology of depression, it is important to use an integrated set of features. This study aimed to determine the relationship between regional brain activity at rest and blood metabolites related to treatment response to escitalopram to identify the characteristics of depression that respond to treatment. METHODS: Blood metabolite levels and resting-state brain activity were measured in patients with moderate to severe depression (n = 65) before and after 6-8 weeks of treatment with escitalopram, and these were compared between Responders and Nonresponders to treatment. We then examined the relationship between blood metabolites and brain activity related to treatment responsiveness in patients and healthy controls (n = 36). RESULTS: Thirty-two patients (49.2%) showed a clinical response (>50% reduction in the Hamilton Rating Scale for Depression score) and were classified as Responders, and the remaining 33 patients were classified as Nonresponders. The pretreatment fractional amplitude of low-frequency fluctuation (fALFF) value of the left dorsolateral prefrontal cortex (DLPFC) and plasma kynurenine levels were lower in Responders, and the rate of increase of both after treatment was correlated with an improvement in symptoms. Moreover, the fALFF value of the left DLPFC was significantly correlated with plasma kynurenine levels in pretreatment patients with depression and healthy controls. CONCLUSION: Decreased resting-state regional activity of the left DLPFC and decreased plasma kynurenine levels may predict treatment response to escitalopram, suggesting that it may be involved in the pathophysiology of major depressive disorder in response to escitalopram treatment. Wiley Psychiatry and clinical neurosciences 1440-1819 76 8 367 376 20220800 10.1111/pcn.13373 35543406 6339428/published_papers/37832841 Thi Kim Ngan Bui Kazuaki Mawatari Takahiro Emoto Shiho Fukushima Takaaki Shimohata Takashi Uebanso Masatake Akutagawa Yohsuke Kinouchi Akira Takahashi UV-LED irradiation reduces the infectivity of herpes simplex virus type 1 by targeting different viral components depending on the peak wavelength. Herpes simplex virus type 1 (HSV-1) is an enveloped virus that mainly infects humans. Given its high global prevalence, disinfection is critical for reducing the risk of infection. Ultraviolet-light-emitting diodes (UV-LEDs) are eco-friendly irradiating modules with different peak wavelengths, but the molecules degraded by UV-LED irradiation have not been clarified. To identify the target viral molecules of UV-LEDs, we exposed HSV-1 suspensions to UV-LED irradiation at wavelengths of 260-, 280-, 310-, and 365-nm and measured viral DNA, protein, and lipid damage and infectivity in host cells. All UV-LEDs substantially reduced by inhibiting host cell transcription, but 260- and 280-nm UV-LEDs had significantly stronger virucidal efficiency than 310- and 365-nm UV-LEDs. Meanwhile, 260- and 280-nm UV-LEDs induced the formation of viral DNA photoproducts and the degradation of viral proteins and some phosphoglycerolipid species. Unlike 260- and 280-nm UV-LEDs, 310- and 365-nm UV-LEDs decreased the viral protein levels, but they did not drastically change the levels of viral DNA photoproducts and lipophilic metabolites. These results suggest that UV-LEDs reduce the infectivity of HSV-1 by targeting different viral molecules based on the peak wavelength. These findings could facilitate the optimization of UV-LED irradiation for viral inactivation. Elsevier BV Journal of photochemistry and photobiology. B, Biology 1011-1344 228 112410 112410 20220300 10.1016/j.jphotobiol.2022.112410 35193038 6339428/published_papers/40832344 Tsubasa Kondo Takashi Uebanso Natsuki Arao Takaaki Shimohata Kazuaki Mawatari Akira Takahashi Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice. Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively. Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis. In the present study, we investigated whether genetic deletion of T1R3 impacted aggravation of DSS-induced colitis in mice. We found that T1R3-KO mice showed reduction in colon damage, including reduced inflammation and colon shrinking relative to those of WT mice following DSS treatment. mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon. Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice. Together, these results indicated that deletion of T1R3 has a minor role in intestinal inflammation induced by DSS-induced acute colitis in mice. Journal of nutritional science and vitaminology 68 3 204 212 20220000 10.3177/jnsv.68.204 35768251 6339428/published_papers/37847579 Shiho Fukushima Takaaki Shimohata Yuri Inoue Junko Kido Takashi Uebanso Kazuaki Mawatari Akira Takahashi Recruitment of LC3 by Campylobacter jejuni to Bacterial Invasion Site on Host Cells via the Rac1-Mediated Signaling Pathway. Campylobacter jejuni is a leading cause of food-borne disease worldwide. The pathogenicity of C. jejuni is closely associated with the internalization process in host epithelial cells, which is related to a host immune response. Autophagy indicates a key role in the innate immune system of the host to exclude invasive pathogens. Most bacteria are captured by autophagosomes and degraded by autophagosome-lysosome fusion in host cells. However, several pathogens, such as Salmonella and Shigella, avoid and/or escape autophagic degradation to establish infection. But autophagy involvement as a host immune response to C. jejuni infection has not been clarified. This study revealed autophagy association in C. jejuni infection. During infection, C. jejuni activated the Rho family small GTPase Rac1 signaling pathway, which modulates actin remodeling and promotes the internalization of this pathogen. In this study, we found the LC3 contribution to C. jejuni invasion signaling via the Rac1 signaling pathway. Interestingly, during C. jejuni invasion, LC3 was recruited to bacterial entry site depending on Rac1 GTPase activation just at the early step of the infection. C. jejuni infection induced LC3-II conversion, and autophagy induction facilitated C. jejuni internalization. Also, autophagy inhibition attenuated C. jejuni invasion step. Moreover, Rac1 recruited LC3 to the cellular membrane, activating the invasion of C. jejuni. Altogether, our findings provide insights into the new function of LC3 in bacterial invasion. We found the interaction between the Rho family small GTPase, Rac1, and autophagy-associated protein, LC3. Frontiers Media SA Frontiers in cellular and infection microbiology 2235-2988 12 829682 829682 20220303 10.3389/fcimb.2022.829682 35310852 6339428/published_papers/35090890 Takashi Uebanso Mai Suyama Takaaki Shimohata Kazuaki Mawatari Akira Takahashi Effect of Vitamin B2-Deficient Diet on Hydroxyproline- or Obesity-Induced Hyperoxaluria in Mice. SCOPE: Hyperoxaluria is a major cause of kidney stone disease. Around half of the oxalate in mammals is supplied from the diet and the other half is endogenously synthesized from glyoxylate. Reduction of hepatic glycolate oxidase (GO) activity is one approach to reduce endogenous production of oxalate. However, there are currently few effective dietary approaches to reduce hepatic GO activity. METHODS AND RESULTS: In the present study, it is investigated whether restriction of dietary vitamin B2 (VB2) can reduce hepatic GO activity and oxalate excretion in mice with hyperoxaluria induce by hydroxyproline (Hyp) or obesity. It is found that VB2 restriction significantly reduces hepatic GO activity in both the Hyp- and obesity-induced model of hyperoxaluria in mice. However, VB2 restriction reduces urinary oxalate excretion only in the Hyp-treated mice and not the obese mice. This difference could be due to the contribution of endogenous oxalate production that manifests as increased hepatic GO activity in Hyp-treated mice but not obese mice. CONCLUSION: Together these results suggest that VB2 restriction could be a new dietary approach to improve hyperoxaluria when endogenous production of oxalate is increased. Molecular nutrition & food research 65 15 e2100226 null 20210800 10.1002/mnfr.202100226 34110671 6339428/published_papers/39265097 Kensuke Horiyama Takahiro Emoto Takeyuki Haraguchi Takashi Uebanso Yuki Naito Takuma Gyobu Kenta Kanemoto Junichi Inobe Ayumi Sano Masatake Akutagawa Akira Takahashi Bowel sound-based features to investigate the effect of coffee and soda on gastrointestinal motility Biomedical Signal Processing and Control 1746-8108 66 null null 20210400 10.1016/j.bspc.2021.102425 2-s2.0-85101785195 6339428/published_papers/35090901 Hiroki Mori Yuji Morine Kazuaki Mawatari Ayumi Chiba Shinichiro Yamada Y U Saito Hiroki Ishibashi Akira Takahashi Mitsuo Shimada Bile Metabolites and Risk of Carcinogenesis in Patients With Pancreaticobiliary Maljunction: A Pilot Study. BACKGROUND/AIM: Pancreaticobiliary maljunction (PBM), a disease with reflux of pancreatic and bile juice in the pancreaticobiliary tract, is a high-risk factor for biliary tract cancer. The aim of this study was to investigate the mechanism of carcinogenesis in PBM using a metabolomics analysis of bile sampled during surgery. PATIENTS AND METHODS: Three patients with PBM without biliary tract cancer, four patients with extrahepatic bile duct cancer (EHBC), and three controls with benign disease were enrolled. Metabolomics analysis of bile samples was performed using capillary electrophoresis-mass spectrometry and liquid chromatography-mass spectrometry to discriminate the amino acid and lipidomic profiles. RESULTS: The principal component analysis in the capillary electrophoresis-mass spectrometry and liquid chromatography-mass spectrometry revealed similar metabolites in patients with PBM and those with EHBC; furthermore, there was a clear difference between patients with PBM or EHBC compared to controls. The amino acid profiles revealed the following 20 potential carcinogenic candidates for PBM: isoleucine, phenylalanine, tyrosine, leucine, tryptophan, arginine, lysine, valine, asparagine, methionine, aspartic acid, serine, threonine, histidine, glutamine, alanine, proline, glutamic acid, and pyruvic acid. The lipidomic profiles revealed the following 11 carcinogenic candidates: lysophosphatidylcholine, lysophosphatidylethanolamine, phosphatidyl glycerol, lysophosphatidyl glycerol, triacylglycerol, diacylglycerol, ceramide, sphyngomyeline, fatty acid, hyperforin, and vitamin D. Among these characteristic metabolites, the branched-chain amino acids, methionine and lysophosphatidylcholine are known to be related to carcinogenesis. CONCLUSION: The bile metabolites were extremely similar in patients with PBM and those with EHBC. Furthermore, amino acid and lipid metabolism was markedly different in patients with PBM or EHBC compared to healthy controls. Anticancer research 41 1 327 334 20210100 10.21873/anticanres.14779 33419827 6339428/published_papers/35090895 Hitomi Iba Takaaki Shimohata Junko Kido Sho Hatayama Takashi Uebanso Kazuaki Mawatari Akira Takahashi Vibrio parahaemolyticus induces inflammation-associated fluid accumulation via activation of the cystic fibrosis transmembrane conductance regulator. Vibrio parahaemolyticus is a foodborne bacterium that causes acute gastroenteritis through the consumption of contaminated, raw, or undercooked seafood. Cystic fibrosis transmembrane conductance regulator (CFTR) is a well-characterized chloride channel that regulates several other ion channels and transporters to maintain water homeostasis in the gut lumen. Also, CFTR is a main target of bacterial infection-associated diarrhea. Hence, the aim of this study was to clarify the contribution of CFTR in V. parahaemolyticus-induced diarrhea in a mouse model of intestinal loop fluid accumulation, with CFTR inhibitors and a CFTR knockout model. The results indicated that CFTR plays a critical role in fluid accumulation in response to V. parahaemolyticus infection. We also investigated the inflammatory association in CFTR-mediated V. parahaemolyticus-induced fluid secretion with cyclooxygenase inhibitors and found that fluid accumulation was decreased by inhibition of cyclooxygenase 2 produced by neutrophils. These findings suggest that V. parahaemolyticus-inducing infiltration and activation of neutrophils also participated in CFTR mediated fluid secretion. This study reveals an important relationship between V. parahaemolyticus-induced diarrhea and inflammation in a mouse model. J. Med. Invest. 68 : 59-70, February, 2021. The journal of medical investigation : JMI 68 1.2 59 70 20210000 10.2152/jmi.68.59 33994481 6339428/published_papers/29543664 Takashi Uebanso Takaaki Shimohata Kazuaki Mawatari Akira Takahashi Functional Roles of B-Vitamins in the Gut and Gut Microbiome. The gut microbiota produce hundreds of bioactive compounds, including B-vitamins, which play significant physiological roles in hosts by supporting the fitness of symbiotic species and suppressing the growth of competitive species. B-vitamins are also essential to the host and certain gut bacterium. Although dietary B-vitamins are mainly absorbed from the small intestine, excess B-vitamins unable to be absorbed in the small intestine are supplied to the distal gut. In addition, B-vitamins are supplied from biosynthesis by distal gut microbiota. B-vitamins in the distal colon may perform many important functions in the body; they act as (1) nutrients for a host and their microbiota, (2) regulators of immune cell activity, (3) mediators of drug efficacy, (4) supporters of survival, or the fitness of certain bacterium, (5) suppressors of colonization by pathogenic bacteria, and (6) modulators of colitis. Insights into basic biophysical principles, including the bioavailability of B-vitamins and their derivatives in the distal gut are still not fully elucidated. Here we briefly review the function of single B-vitamin in the distal gut including their roles in relation to bacteria. The prospect of extending analytical methods to better understand the role of B-vitamins in the gut is also explored. This article is protected by copyright. All rights reserved. Molecular nutrition & food research 64 18 e2000426 null 20200900 10.1002/mnfr.202000426 32761878 6339428/published_papers/43062486 Toshiya Okahisa Masahiro Sogabe Tadahiko Nakagawa Kumiko Tanaka Tetsu Tomonari Tatsuya Taniguchi Akira Takahashi Yohsuke Kinouchi Junji Nishioka Naoki Igata Hiroaki Yanagawa Takatoshi Komatsu Yoshiaki Ohnishi Masashi Fukuhara Masashi Ishikawa Hiroshi Shibata Hirohiko Shinomiya Masahiko Nakasono Fumiko Kishi Keiko Komai Yayoi Tatsuki Toru Murashima Yoshihiro Deguchi Hiroshi Aramaki Hideyuki Fukumitsu Tetsuji Takayama Development of a novel automatic ascites filtration and concentration equipment with multi-ring-type roller pump units for cell-free and concentrated ascites reinfusion therapy. Cell-free and concentrated ascites reinfusion therapy (CART) is an effective therapy for refractory ascites. However, CART is difficult to perform as ascites filtration and concentration is a complicated procedure. Moreover, the procedure requires the constant assistance of a clinical engineer or/and the use of an expensive equipment for the multi-purpose blood processing. Therefore, we developed a CART specialized equipment (mobility CART [M-CART]) that could be used safely with various safety measures and automatic functions such as automatic washing of clogged filtration filter and self-regulation of the concentration ratio. Downsizing, lightning of the weight, and automatic processing in M-CART required the use of newly developed multi-ring-type roller pump units. This equipment was approved under Japanese regulations in 2018. In performing 41 sessions of CART (for malignant ascites, 22 sessions; and hepatic ascites, 19 sessions) using this equipment in 17 patients, no serious adverse event occurred. An average of 4494 g of ascites was collected and the total amount of ascites was processed in all the sessions without any trouble. The mean weight of the processed ascites was 560 g and the mean concentration ratio was 8.0. The ascites were processed at a flow rate of 50 mL/min. The mean ascites processing time was 112.5 minutes and a 106.5-minutes (95.2%) ascites processing was performed automatically. The operator responded to alarms or support information 3.2 times on average (3.1 minutes, 2.1% of ascites processing time). Human errors related to ascites processing were detected by M-CART at 0.4 times per session on average and were appropriately addressed by the operator. The frequencies of automatic washing of clogged filtration filter and self-regulation of the concentration ratio were 31.7% and 53.7%, respectively. The mean recovery rates (recovery dose) of protein, albumin, and immunoglobulin G were 72.9%, 72.9%, and 71.2% (65.9 g, 34.9 g, and 13.2 g), respectively. Steroids were administered in 92.7% of the sessions to prevent fever and the mean increase in body temperature was 0.53°C. M-CART is a compact and lightweight automatic CART specialized equipment that can safely and easily process a large quantity of ascites without the constant assistance of an operator. Artificial organs 44 8 856 872 20200800 10.1111/aor.13681 32187379 6339428/published_papers/29543667 Mizuki Kojima Kazuaki Mawatari Takahiro Emoto Risa Nishisaka-Nonaka Thi Kim Ngan Bui Takaaki Shimohata Takashi Uebanso Masatake Akutagawa Yohsuke Kinouchi Takahiro Wada Masayuki Okamoto Hiroshi Ito Kenji Tojo Tomo Daidoji Takaaki Nakaya Akira Takahashi Irradiation by a Combination of Different Peak-Wavelength Ultraviolet-Light Emitting Diodes Enhances the Inactivation of Influenza A Viruses. Influenza A viruses (IAVs) pose a serious global threat to humans and their livestock. This study aimed to determine the ideal irradiation by ultraviolet-light emitting diodes (UV-LEDs) for IAV disinfection. We irradiated the IAV H1N1 subtype with 4.8 mJ/cm2 UV using eight UV-LEDs [peak wavelengths (WL) = 365, 310, 300, 290, 280, 270, and 260 nm)] or a mercury low pressure (LP)-UV lamp (Peak WL = 254 nm). Inactivation was evaluated by the infection ratio of Madin-Darby canine kidney (MDCK) cells or chicken embryonated eggs. Irradiation by the 260 nm UV-LED showed the highest inactivation among all treatments. Because the irradiation-induced inactivation effects strongly correlated with damage to viral RNA, we calculated the correlation coefficient (RAE) between the irradiant spectrum and absorption of viral RNA. The RAE scores strongly correlated with the inactivation by the UV-LEDs and LP-UV lamp. To increase the RAE score, we combined three different peak WL UV-LEDs (hybrid UV-LED). The hybrid UV-LED (RAE = 86.3) significantly inactivated both H1N1 and H6N2 subtypes to a greater extent than 260 nm (RAE = 68.6) or 270 nm (RAE = 42.2) UV-LEDs. The RAE score is an important factor for increasing the virucidal effects of UV-LED irradiation. Microorganisms 8 7 null null 20200708 10.3390/microorganisms8071014 32650492 6339428/published_papers/25935494 Anh Quoc Nguyen Takaaki Shimohata Sho Hatayama Aya Tentaku Junko Kido Thi Mai Huong Bui Takashi Uebanso Kazuaki Mawatari Akira Takahashi Type III Secretion Effector VopQ of Vibrio parahaemolyticus Modulates Central Carbon Metabolism in Epithelial Cells. Vibrio parahaemolyticus is a Gram-negative halophilic pathogen that frequently causes acute gastroenteritis and occasional wound infection. V. parahaemolyticus contains several virulence factors, including type III secretion systems (T3SSs) and thermostable direct hemolysin (TDH). In particular, T3SS1 is a potent cytotoxic inducer, and T3SS2 is essential for causing acute gastroenteritis. Although much is known about manipulation of host signaling transductions by the V. parahaemolyticus effector, little is known about the host metabolomic changes modulated by V. parahaemolyticus To address this knowledge gap, we performed a metabolomic analysis of the epithelial cells during V. parahaemolyticus infection using capillary electrophoresis-time of flight mass spectrometry (CE-TOF/MS). Our results revealed significant metabolomic perturbations upon V. parahaemolyticus infection. Moreover, we identified that T3SS1's VopQ effector was responsible for inducing the significant metabolic changes in the infected cells. The VopQ effector dramatically altered the host cell's glycolytic, tricarboxylic acid cycle (TCA), and amino acid metabolisms. VopQ effector disrupted host cell redox homeostasis by depleting cellular glutathione and subsequently increasing the level of reactive oxygen species (ROS) production.IMPORTANCE The metabolic response of host cells upon infection is pathogen specific, and infection-induced host metabolic reprogramming may have beneficial effects on the proliferation of pathogens. V. parahaemolyticus contains a range of virulence factors to manipulate host signaling pathways and metabolic processes. In this study, we identified that the T3SS1 VopQ effector rewrites host metabolism in conjunction with the inflammation and cell death processes. Understanding how VopQ reprograms host cell metabolism during the infection could help us to identify novel therapeutic strategies to enhance the survival of host cells during V. parahaemolyticus infection. mSphere 5 2 null null 20200318 10.1128/mSphere.00960-19 32188755 6339428/published_papers/25935497 Takashi Uebanso Ayumi Yoshimoto Shinta Aizawa Maya Nakamura Rumiko Masuda Takaaki Shimohata Kazuaki Mawatari Akira Takahashi Glycolate is a Novel Marker of Vitamin B2 Deficiency Involved in Gut Microbe Metabolism in Mice. Microbes in the human gut play a role in the production of bioactive compounds, including some vitamins. Although several studies attempted to identify definitive markers for certain vitamin deficiencies, the role of gut microbiota in these deficiencies is unclear. To investigate the role of gut microbiota in deficiencies of four vitamins, B2, B6, folate, and B12, we conducted a comprehensive analysis of metabolites in mice treated and untreated with antibiotics. We identified glycolate (GA) as a novel marker of vitamin B2 (VB2) deficiency, and show that gut microbiota sense dietary VB2 deficiency and accumulate GA in response. The plasma GA concentration responded to reduced VB2 supply from both the gut microbiota and the diet. These results suggest that GA is a novel marker that can be used to assess whether or not the net supply of VB2 from dietary sources and gut microbiota is sufficient. We also found that gut microbiota can provide short-term compensation for host VB2 deficiency when dietary VB2 is withheld. Nutrients 12 3 null null 20200311 10.3390/nu12030736 32168816 6339428/published_papers/29543803 Maria Ulfa Ulfa Maria Momoyo Azuma Emoto Takahiro Masami Sato Akutagawa Masatake Takaaki Shimohata Kinouchi Yohsuke Shiho Fukushima Takahashi Akira Junko Kido Azuma Momoyo Mariko Nakamoto Sato Masami Takashi Uebanso Shimohata Takaaki Kazuaki Mawatari Fukushima Shiho Takahiro Emoto Kido Junko Masatake Akutagawa Nakamoto Mariko Yohsuke Kinouchi Uebanso Takashi Akira Takahashi Mawatari Kazuaki Inactivation of Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli by UVA-LED irradiation system. Inactivation of Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli by UVA-LED irradiation system <p>The prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli is increasing rapidly and spreading worldwide, particularly in Asia, compared to other regions. In the last ten years, in our hospital, in particular, there has been a < 30% increase. To prevent the spread of ESBL in hospitals and the community, the ultraviolet (UV) A-light-emitting diode (LED) irradiation device was used to inactivate ESBL-E. coli in human livestock and the environment. ESBL-E. coli and E. coli bacterial samples were collected from patients at Tokushima University Hospital (Tokushima City, Japan). The UVA-LED irradiation system had 365 nm single wavelength, and the current of the circuit was set to 0.23 or 0.50 A consistently. Results demonstrated that UVA-LED was useful for the inactivation of ESBL-E. coli and E. coli. The minimum energy dosage required to inactivate ESBL-E. coli and E. coli was 40.76 J/cm2 (45 min) in the first type of UVA-LED and 38.85 J/cm2 (5 min) in the second type. There were no significant differences between ESBL-E. coli and E. coli. The inactivation of ESBL-E. coli was dependent on energy. These findings suggest that UVA-LED with 365 nm single wavelength could be useful for surface decontamination in healthcare facilities. J. Med. Invest. 67 : 163-169, February, 2020</p> The University of Tokushima Faculty of Medicine 国立大学法人 徳島大学医学部 The journal of medical investigation : JMI The Journal of Medical Investigation 1343-1420 67 1.2 163 169 20200000 10.2152/jmi.67.163 32378601 6339428/published_papers/5037207 Hiromichi Yumoto Takashi Uebansou Takaaki Shimohata Akira Takahashi Current understanding of the gut microflora in subjects with nutrition-associated metabolic disorder such as obesity and/or diabetes: Is there any relevance with oral microflora? Current Oral Health Reports 2196-3002 6 2 100 109 20190600 10.1007/s40496-019-0221-7 6339428/published_papers/5037208 Takaaki Shimohata Kazuaki Mawatari Takashi Uebansou Airi Honjo Akari Tsunedomi Sho Hatayama Yuri Sato Junko Kido Risa Nishisaka Ayumi Yoshimoto Tomoko Yamashita Sachie Amano Miki Maetani-Yasui Hitomi Iba Yumi Harada Mutsumi Nakahashi Sonoko Yamada Yasuhiro Hamada Tadahiko Nakagawa Masahiro Sogabe Takahiro Emoto Masatake Akutagawa Toshiya Okahisa Yohsuke Kinouchi Akira Takahashi Bacterial Contamination of Hemodialysis Devices in Hospital Dialysis Wards. Chronic care patients undergoing hemodialysis for treatment of end-stage renal failure experience higher rates of bloodstream-associated infection due to the patients&#039; compromised immune system and management of the bloodstream through catheters. Staphylococcus species are acommon cause of hemodialysis catheterrelated bloodstream infections. We investigated environmental bacterial contamination of dialysis wards and contamination of hemodialysis devices to determine the source of bacteria for these infections. All bacterial samples were collected by the swab method and the agarose stamp method. And which bacterium were identified by BBL CRYSTAL Kit or 16s rRNA sequences. In our data, bacterial cell number of hemodialysis device was lower than environment of patient surrounds. But Staphylococcus spp. were found predominantly on the hemodialysis device (46.8%), especially on areas frequently touched by healthcare-workers (such as Touch screen). Among Staphylococcus spp., Staphylococcus epidermidis was most frequently observed (42.1% of Staphylococcus spp.), and more surprising, 48.2% of the Staphylococcus spp. indicated high resistance for methicillin. Our finding suggests that hemodialysis device highly contaminated with bloodstream infection associated bacteria. This study can be used as a source to assess the risk of contamination-related infection and to develop the cleaning system for the better prevention for bloodstream infections in patients with hemodialysis. J. Med. Invest. 66 : 148-152, February, 2019. The Journal of Medical Investigation : JMI 1349-6867 66 1.2 148 152 20190000 10.2152/jmi.66.148 31064928 6339428/published_papers/24816051 Takaaki Shimohata Kazuaki Mawatari Takashi Uebanso Airi Honjo Akari Tsunedomi Sho Hatayama Yuri Sato Junko Kido Risa Nishisaka Ayumi Yoshimoto Tomoko Yamashita Sachie Amano Miki Maetani-Yasui Hitomi Iba Yumi Harada Mutsumi Nakahashi Sonoko Yasui-Yamada Yasuhiro Hamada Tadahiko Nakagawa Masahiro Sogabe Takahiro Emoto Masatake Akutagawa Toshiya Okahisa Yohsuke Kinouchi Akira Takahashi Bacterial Contamination of Hemodialysis Devices in Hospital Dialysis Wards. The journal of medical investigation : JMI 1349-6867 66 1.2 148 152 20190000 10.2152/jmi.66.148 31064928 2-s2.0-85065758123 6339428/published_papers/24816048 Risa Nishisaka-Nonaka Kazuaki Mawatari Tomomi Yamamoto Mizuki Kojima Takaaki Shimohata Takashi Uebanso Mutsumi Nakahashi Takahiro Emoto Masatake Akutagawa Yohsuke Kinouchi Takahiro Wada Masayuki Okamoto Hiroshi Ito Ken-Ichi Yoshida Tomo Daidoji Takaaki Nakaya Akira Takahashi Irradiation by ultraviolet light-emitting diodes inactivates influenza a viruses by inhibiting replication and transcription of viral RNA in host cells. Journal of photochemistry and photobiology. B, Biology 1873-2682 189 193 200 20181200 10.1016/j.jphotobiol.2018.10.017 30391908 2-s2.0-85055909850 6339428/published_papers/5037211 Ayumi Yoshimoto Takashi Uebanso Mutsumi Nakahashi Takaaki Shimohata Kazuaki Mawatari Akira Takahashi Effect of prenatal administration of low dose antibiotics on gut microbiota and body fat composition of newborn mice. Journal of clinical biochemistry and nutrition 0912-0009 62 2 155 160 20180300 10.3164/jcbn.17-53 29610555 2-s2.0-85043523839 6339428/published_papers/5037210 Sho Hatayama Takaaki Shimohata Sachie Amano Junko Kido Anh Q. Nguyen Yuri Sato Yuna Kanda Aya Tentaku Shiho Fukushima Mutsumi Nakahashi Takashi Uebanso Kazuaki Mawatari Akira Takahashi Cellular tight junctions prevent effective Campylobacter jejuni invasion and inflammatory barrier disruption promoting bacterial invasion from lateral membrane in polarized intestinal epithelial cells Frontiers in Cellular and Infection Microbiology 2235-2988 8 JAN null null 20180130 10.3389/fcimb.2018.00015 29441328 2-s2.0-85041351089 6339428/published_papers/5037209 Aya Tentaku Takaaki Shimohata Sho Hatayama Junko Kido Anh Quoc Nguyen Yuna Kanda Shiho Fukushima Takashi Uebanso Taketoshi Iwata Kazuaki Mawatari Nagakatsu Harada Akira Takahashi Host cellular unfolded protein response signaling regulates Campylobacter jejuni invasion. PloS one 1932-6203 13 10 e0205865 null 20181001 10.1371/journal.pone.0205865 30321237 2-s2.0-85055071287 6339428/published_papers/24816049 Akari Tsunedomi Katsuyuki Miyawaki Akinori Masamura Mutsumi Nakahashi Kazuaki Mawatari Takaaki Shimohata Takashi Uebanso Yousuke Kinouchi Masatake Akutagawa Takahiro Emoto Akira Takahashi UVA-LED device to disinfect hydroponic nutrient solution. The journal of medical investigation : JMI 1349-6867 65 3.4 171 176 20180000 10.2152/jmi.65.171 30282856 2-s2.0-85054465405 6339428/published_papers/54141296 天宅 あや 下畑 隆明 畑山 翔 Nguyen Anh 木戸 純子 福島 志帆 上番増 喬 馬渡 一章 高橋 章 Campylobacter jejun感染で誘導される小胞体ストレス応答は細胞内への菌の侵入を抑制する 生命科学系学会合同年次大会運営事務局 生命科学系学会合同年次大会 2017 [3LBA 105] 20171200 6339428/published_papers/24816055 Junko Kido Kido J Takaaki Shimohata Shimohata T Sachie Amano Amano S Sho Hatayama Hatayama S Anh Quoc Nguyen Nguyen AQ Yuri Sato Sato Y Yuna Kanda Kanda Y Aya Tentaku Tentaku A Shiho Fukushima Fukushima S Mutsumi Nakahashi Nakahashi M Takashi Uebanso Uebanso T Kazuaki Mawatari Mawatari K Akira Takahashi Takahashi A Cystic Fibrosis Transmembrane Conductance Regulator Reduces Microtubule-Dependent Campylobacter jejuni Invasion. Infection and immunity 1098-5522 85 10 null null 20171000 10.1128/IAI.00311-17 28784926 2-s2.0-85029769185 6339428/published_papers/5037212 Junko Kido Takaaki Shimohata Sachie Amano Syo Hatayama Quoc Anh Nguyen Yuri Sato Yuna Kanda Aya Tentaku Shiho Fukushima Mutsumi Nakahashi Takashi Uebansou Kazuaki Mawatari Akira Takahashi CFTR reduces microtubule-dependent Campylobacter jejuni invasion. Campylobacter jejuni (C. jejuni) is gastroenteritis inducible food-born pathogen. Invasion and adhesion process are essential for leading gastroenteritis in C. jejuni infection process. As against bacterial strategy for efficacy invasion and adhesion, mucosal layer play a key role in defense systems, which modulated by several ion channels and transporters mediated water flux on the intestine. Cystic fibrosis transmembrane conductance regulator (CFTR) play the main role in waterfulux in intestine, and it closely related with bacterial clearance. We previously reported that C. jejuni infection suppresses CFTR channel activity in intestinal epithelial cells, however the mechanism and importance of this suppression is unclear. This study seeks to elucidate the role of CFTR in C. jejuni-infection. Using HEK293 cells that stably express wild type and mutated CFTR, we found that CFTR attenuated C. jejuni invasion, it was not involved bacterial adhesion or intracellular survival but associated with microtubule-dependent cellular transport. Moreover we revealed that CFTR attenuated function of microtubule motor protein but not microtubule stability, which causes inhibition of C. jejuni-invasion. Meanwhile, the CFTR mutant G551D-CFTR, which has defects in channel activity, suppressed C. jejuni-invasion, whereasF508-CFTR, which has defects in maturation, did not suppress, suggesting that CFTR suppression of C. jejuni-invasion is related to CFTR maturation but not channel activity.Taken together, mature CFTR inhibited C. jejuni invasion by regulating microtubule-mediated pathways. We suggest that CFTR plays a critical role in cellular defenses against C. jejuni-invasion, and CFTR suppression may be an initial step in promoting cellular invasion during C. jejuni-infection. Infection and Immunity 1098-5522 null null 20170807 10.1128/IAI.00311-17 28784926 6339428/published_papers/5037213 Takashi Uebanso Saki Kano Ayumi Yoshimoto Chisato Naito Takaaki Shimohata Kazuaki Mawatari Akira Takahashi Effects of consuming xylitol on gut microbiota and lipid metabolism in mice Nutrients 2072-6643 9 7 756 756 20170714 10.3390/nu9070756 28708089 2-s2.0-85025080133 6339428/published_papers/5037214 Takashi Uebanso Ai Ohnishi Reiko Kitayama Ayumi Yoshimoto Mutsumi Nakahashi Takaaki Shimohata Kazuaki Mawatari Akira Takahashi Effects of low-dose non-caloric sweetener consumption on gut microbiota in mice Nutrients 2072-6643 9 6 null null 20170600 10.3390/nu9060662 28587159 2-s2.0-85020307595 6339428/published_papers/5037215 Yanfei Hou Mutsumi Nakahashi Kazuaki Mawatari Takaaki Shimohata Takashi Uebansou Yumi Harada Akari Tsunedomi Takahiro Emoto Masatake Akutagawa Yohsuke Kinouchi Akira Takahashi Combined treatment of UVA irradiation and antibiotics induces greater bactericidal effects on Vibrio parahaemolyticus. The presence of antibiotics in the environment and their subsequent impact on the development of multi-antibiotic resistant bacteria has raised concerns globally. Consequently, much research is focused on a method to produce a better disinfectant. We have established a disinfectant system using UVA-LED that inactivates pathogenic bacteria. We assessed the bactericidal efficiency of a combination of UVA-LED and antibiotics against Vibrio parahaemolyticus. Combined use of antibiotic drugs and UVA irradiation was more bactericidal than UVA irradiation or antibacterial drugs alone. The bactericidal synergy was observed at low concentrations of each drug that are normally unable to kill the bacteria. This combination has the potential to become a sterilization technology. J. Med. Invest. 63: 63-67, February, 2016. The Journal of Medical Investigation : JMI 1349-6867 63 1-2 63 67 20160000 10.2152/jmi.63.63 27040055 6339428/published_papers/5037216 Quang Ngoc Phan Takashi Uebansou Takaaki Shimohata Mutsumi Nakahashi Kazuaki Mawatari Akira Takahashi DNA-binding protein HU coordinates pathogenicity in Vibrio parahaemolyticus. Nucleoid binding protein HU regulate cellular behaviors, including nucleoid structuring, general recombination, transposition, growth, replication, motility, metabolism, and virulence. It is concerned that both number of bacteria and number of virulence factors may affect pathogenecity of bacteria. In the present study, we investigated that which factor have dominant role during infection in one of the most rapidly growing bacteria Vibrio parahaemolyticus. We found that V. parahaemolyticus cytotoxicity is regulated, in a growth rate-independent manner, by the HU proteins through regulation of number of virulence factors including T3SS1 gene expression. Journal of Bacteriology 1098-5530 null null 20150706 10.1128/JB.00306-15 26148713 6339428/published_papers/5037217 Toshitaka Ikehara Mutsumi Nakahashi Su Zehong Masatake Akutagawa Koichiro Tsuchiya Mitsuo Kitamura Akira Takahashi Yohsuke Kinouchi Effects of UV-A LED light irradiation on growth of cultured RAW 264.7 cells TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY 1029-0486 97 2 243 255 20150200 10.1080/02772248.2015.1039771 6339428/published_papers/5037218 Yasuko Kadomura-Ishikawa Katsuyuki Miyawaki Akira Takahashi Toshiya Masuda Sumihare Noji Light and abscisic acid independently regulated FaMYB10 in Fragaria × ananassa fruit Springer Verlag Planta 1432-2048 241 4 953 965 20150000 10.1007/s00425-014-2228-6 25534946 2-s2.0-84925538505 6339428/published_papers/5037301 Noriko Matsumoto Kouzou Yoshikawa Mitsuo Shimada Nobuhiro Kurita Horohiko Sato Takashi Iwata Jun Higashijima Motoya Chikakiyo Masaaki Nishi Hideya Kashihara Chie Takasu Shohei Eto Akira Takahashi Masatake Akutagawa Takahiro Emoto Effect of light irradiation by light emitting diode on colon cancer cells. Recent studies have demonstrated the efficacy of irradiation from light emitting diodes (LED) for wound healing, anti-inflammation and anticancer therapies. However, little is known about the effects of visible light in colon cancer cells. The purpose of this study was to evaluate the biological response (including gene expression changes) of human colon cancer cells to different wavelengths of LED irradiation. Human colon cancer cells (HT29 or HCT116) were seeded onto laboratory dishes that were then put on LED irradiation equipment with a 465 nm-, 525 nm-, or 635 nm-LED. Irradiation at 15 or 30 mW was performed 10 min/day, each day for 5 days. The cell counting kit8 was then used to measure cell viability. Apoptosis and expression of several mRNAs (caspase, MAPK and autophagy pathway) in HT29 cultures irradiated with 465 nm LED were evaluated via AnnexinV/PI and RT-PCR, respectively. Viability of HT29 and HCT116 cells was lower in 465 nm-LED irradiated cultures than in control cultures, but viability of HT29 cells did not differ between control cultures and 525 nm-LED or 635 nm-LED irradiated cultures. Moreover, the expression of FAS, caspase-3, capase-8, and JUK were significantly higher in 465 nm-LED irradiated cultures than in control cultures, and expression of ERK1/2 and LC3 was lower in blue-irradiated cells. LED irradiation at 465 nm inhibited the proliferation of HT29 cells and of HCT116 cells. Notably, LED irradiation at 465 nm promoted apoptosis inHT29 cultures via the extrinsic apoptosis pathway and the MAPK pathway. Anticancer Research 1791-7530 34 9 4709 4716 20140900 25202048 6339428/published_papers/5037219 Sachie Negoro Takaaki Shimohata Syo Hatayama Yuri Sato Mari Matsumoto Hitomi Iba Mutsumi Aihara Takashi Uebansou Yasuhiro Hamada Yoshikazu Nishikawa Shinji Yamasaki Kazuaki Mawatari Akira Takahashi Campylobacter jejuni infection suppressed Cl(-) secretion induced by CFTR activation in T-84 cells. Campylobacter jejuni causes foodborne disease associated with abdominal pain, gastroenteritis, and diarrhea. These symptoms are induced by bacterial adherence and invasion of host epithelial cells. C. jejuni infection can occur with a low infective dose, suggesting that C. jejuni may have evolved strategies to cope with the bacterial clearance system in the gastrointestinal tract. The mucosa layer is the first line of defense against bacteria. Mucus conditions are maintained by water and anion (especially Cl(-)) movement. Cystic fibrosis transmembrane conductance regulator (CFTR) is the main Cl(-) channel transporting Cl(-) to the lumen. Mutations in CFTR result in dehydrated secreted mucus and bacterial accumulation in the lungs, and recent studies suggest that closely related pathogenic bacteria also may survive in the intestine. However, the relationship between C. jejuni infection and CFTR has been little studied. Here, we used an (125)I(-) efflux assay and measurement of short-circuit current to measure Cl(-) secretion in C. jejuni-infected T-84 human intestinal epithelial cells. The basic state of Cl(-) secretion was unchanged by C. jejuni infection, but CFTR activator was observed to induce Cl(-) secretion suppressed in C. jejuni-infected T-84 cells. The suppression of activated Cl(-) secretion was bacterial dose-dependent and duration-dependent. A similar result was observed during infection with other C. jejuni strains. The mechanism of suppression may occur by affecting water movement or mucus condition in the intestinal tract. A failure of mucus barrier function may promote bacterial adhesion or invasion of host intestinal epithelial cells, thereby causing bacterial preservation in the host intestinal tract. Journal of Infection and Chemotherapy 1437-7780 20 11 682 688 20140806 10.1016/j.jiac.2014.07.007 25107576 6339428/published_papers/5037220 Mutsumi Nakahashi Kazuaki Mawatari Akiko Hirata Miki Maetani Takaaki Shimohata Takashi Uebansou Yasuhiro Hamada Masatake Akutagawa Yohsuke Kinouchi Akira Takahashi Simultaneous irradiation with different wavelengths of ultraviolet light has synergistic bactericidal effect on Vibrio parahaemolyticus. Ultraviolet (UV) irradiation is an increasingly used method of water disinfection. UV rays can be classified by wavelength into UVA (320-400 nm), UVB (280-320 nm), and UVC (&lt; 280 nm). We previously developed UVA sterilization equipment with a UVA-light emitting diode (LED). The aim of this study was to establish a new water disinfection procedure using the combined irradiation of the UVA-LED and another UV wavelength. An oxidative DNA product, 8-hydroxy-2&#039;-deoxyguanosine (8-OHdG), increased after irradiation by UVA-LED alone, and the level of cyclobutane pyrimidine dimers (CPDs) was increased by UVC alone in Vibrio parahaemolyticus. Although sequential irradiation of UVA-LED and UVC induced additional bactericidal effects, simultaneous irradiation with UVA-LED and UVC induced bactericidal synergistic effects. 8-OHdG and CPDs production showed no differences between sequential and simultaneous irradiation. Interestingly, the recovery of CPDs was delayed by simultaneous irradiation. The synergistic effect was absent in SOS response-deficient mutants, such as the recA and lexA strains. Because recA- and lexA-mediated SOS responses have crucial roles in a DNA repair pathway, the synergistic bactericidal effect produced by the simultaneous irradiation could depend on suppression of the CPDs repair. The simultaneous irradiation of UVA-LED and UVC is a candidate new procedure for effective water disinfection. This article is protected by copyright. All rights reserved. Photochemistry and Photobiology 1751-1097 90 6 1397 1403 20140704 10.1111/php.12309 25041035 6339428/published_papers/5037222 Kazuaki Mawatari Emiko Yoshioka Satomi Toda Sonoko Yasui Hiroko Furukawa Takaaki Shimohata Takamasa Ohnishi Masaki Morishima Nagakatsu Harada Akira Takahashi Hiroshi Sakaue Yutaka Nakaya Enhancement of endothelial function inhibits left atrial thrombi development in an animal model of spontaneous left atrial thrombosis. Left atrial (LA) thrombosis is an important cause of systemic embolization. The SPORTS rat model of LA thrombi (Spontaneously-Running Tokushima-Shikoku), which have a unique characteristic of high voluntary wheel running, was previously established. The aim of the present study was to investigate how SPORTS rats develop LA thrombi.Methods and Results:Nitric oxide (NO) produced from cardiovascular endothelial cells plays an important protective role in the local regulation of blood flow, vascular tone, and platelet aggregation. No evidence of atrial fibrillation or hypercoagulability in SPORTS rats regardless of age was found; however, SPORTS rats demonstrated endothelial dysfunction and a decrease of NO production from a young age. In addition, endothelial NO synthase activity was significantly decreased in the LA and thoracic aorta endothelia of SPORTS rats. While voluntary wheel running was able to intermittently increase NO levels, running did not statistically decrease the incidence of LA thrombi at autopsy. However, L-arginine treatment significantly increased NO production and provided protection from the development of LA thrombi in SPORTS rats. They present study results indicate that NO has an important role in the development of LA thrombus, and endothelia pathways could provide new targets of therapy to prevent LA thrombosis. (Circ J 2014; 78: 1980-1988). Circulation Journal 1347-4820 78 8 1980 1988 20140523 10.1253/circj.CJ-13-1398 24859498 6339428/published_papers/5037223 Akihiro Shirai Mutsumi Aihara Akira Takahashi Hideaki Maseda Takeshi Omasa Synergistic antimicrobial activity based on the combined use of a gemini-quaternary ammonium compound and ultraviolet-A light Journal of Photochemistry and Photobiology B: Biology 1011-1344 130 226 233 20140105 10.1016/j.jphotobiol.2013.11.027 24362318 2-s2.0-84890840874 6339428/published_papers/5037224 Mutsumi Aihara Xin Lian Takaaki Shimohata Takashi Uebansou Kazuaki Mawatari Yumi Harada Masatake Akutagawa Yohsuke Kinouchi Akira Takahashi Vegetable surface sterilization system using UVA light-emitting diodes. Surface sterilization of fresh produce has been needed in the food manufacturing/processing industry. Here we report a UVA-LED (Ultra Violet A-Light Emitting Diode) system for surface sterilization that is safe, efficacious, low cost, and apparently harmless to fresh produce. To test the system, Escherichia coli strain DH5 was spot-inoculated onto vegetable tissues, and treated under UVA-LED. Tissues were homogenized and bacteria quantified by colony-forming assay. Possible effects of UVA-LED on vegetable quality were evaluated by HPLC. Tissue weight changes were checked after treatment at 4, 15, and 30. Bacterial inactivation by UVA-LED radiation was observed after a 10 min treatment and increased with increasing time of irradiation. The log survival ratio reached -3.23 after a 90 min treatment. Bacterial cells surviving treatment grew slowly compared to non-irradiated control cells. Cabbage tissue lost weight over time after treatment, and weight loss increased with increasing incubation temperature, but there was no difference between losses by UVA-LED treated and control tissues at any temperature tested. In addition, no differences of Vitamin C content in cabbage tissue were detected by HPLC after UVA-LED treatment. These results suggest that UVA-LED treatment has great potential for vegetable surface sterilization in the food manufacturing/processing industry. The Journal of Medical Investigation : JMI 1349-6867 61 3-4 285 290 20140000 10.2152/jmi.61.285 25264046 6339428/published_papers/5037221 Yuya Manabe 真鍋 祐矢 Miki Maetani 前谷 実希 Akihiko Nagano 長野 明彦 Kenji Teranishi 寺西 研二 Naoyuki Shimomura 下村 直行 Akira Takahashi 橋 章 Influences of pulsed electric fields on the gene expression of pathogenic bacteria 病原性細菌の遺伝子発現に対するパルス電界印加の影響に関する研究 Institute of Electrical Engineers of Japan IEEJ Transactions on Fundamentals and Materials 電気学会論文誌A (基礎・材料・共通部門誌) 1347-5533 134 6 3 396 20140000 10.1541/ieejfms.134.390 2-s2.0-84901780177 6339428/published_papers/5037225 Y Kadomura-Ishikawa Katsuyuki Miyawaki Sumihare Noji Akira Takahashi Phototropin 2 is involved in blue light-induced anthocyanin accumulation in Fragaria x ananassa fruits Anthocyanins are widespread, essential secondary metabolites in higher plants during color development in certain flowers and fruits. In strawberries, anthocyanins are also key contributors to fruit antioxidant capacity and nutritional value. However, the effects of different light qualities on anthocyanin accumulation in strawberry (Fragaria x ananassa, cv. Sachinoka) fruits remain elusive. In the present study, we showed the most efficient increase in anthocyanin content occurred by blue light irradiation. Light sensing at the molecular level was investigated by isolation of two phototropin (FaPHOT1 and FaPHOT2), two cryptochrome (FaCRY1 and FaCRY2), and two phytochrome (FaPHYA and FaPHYB) homologs. Expression analysis revealed only FaPHOT2 transcripts markedly increased depending on fruit developmental stage, and a corresponding increase in anthocyanin content was detected. FaPHOT2 knockdown resulted in decreased anthocyanin content; however, overexpression increased anthocyanin content. These findings suggested blue light induced anthocyanin accumulation, and FaPHOT2 may play a role in sensing blue light, and mediating anthocyanin biosynthesis in strawberry fruits. This is the first report to find a relationship between visible light sensing, and color development in strawberry fruits. Journal of Plant Research 1618-0860 126 6 847 857 20131100 10.1007/s10265-013-0582-2 23982948 6339428/published_papers/5037227 HASHIMOTO Atsushi MAWATARI Kazuaki KINOUCHI Yohsuke AKUTAGAWA Masatake OTA Naotomo NISHIMURA Kazuyuki HIRATA Tsuyoshi TAKAHASHI Akira Inactivation of MS2 Phage and <I>Cryptosporidium parvum</I> Oocysts Using UV-A from High-Intensity Light-Emitting Diode for Water Disinfection In this study, high-intensity, UV-A (ranging from 360 to 370 nm, peak wavelength at 365 nm) produced by a light-emitting diode was used for the inactivation of MS2 phage and <I>Cryptosporidium parvum</I> oocyst. In the irradiation experiment with MS2 phage, approximately 44 and 65 J/cm<SUP>2</SUP> of UV-A were required to obtain -2 and -3 log inactivations, respectively. The -2 and -3 log inactivations of <I>Cryptosporidium</I> oocysts required 338 and 508 J/cm<SUP>2</SUP> UV-A, respectively, which were 7.7 - 7.8 times greater than those required for MS2 phage. The possibility that high-intensity UV-A irradiation can inactivate both protozoa and viruses (phage) was demonstrated in this study. Japan Society on Water Environment 公益社団法人 日本水環境学会 Journal of Water and Environment Technology 1348-2165 11 4 299 307 20130000 10.2965/jwet.2013.299 6339428/published_papers/5037226 Takafumi Yakabe Takaaki Shimohata Akira Takahashi Lactobacillus brevis KB290 enhances IL-8 secretion by Vibrio parahaemolyticus-infected caco-2 cells Journal of Microbiology and Biotechnology 1017-7825 23 1 118 124 20130100 10.4014/jmb.1207.07020 23314377 2-s2.0-84873649315 6339428/published_papers/5037229 Tam Thi Thanh Le Kazuaki Mawatari Miki Maetani Tomomi Yamamoto Sayaka Hayashida Hitomi Iba Mutsumi Aihara Akiko Hirata Takaaki Shimohata Takashi Uebanso Akira Takahashi VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1872-8006 1820 10 1686 1692 20121000 10.1016/j.bbagen.2012.06.019 22771831 6339428/published_papers/5037228 Takaaki Shimohata Kazuaki Mawatari Hitomi Iba Masakazu Hamano Sachie Negoro Shoko Asada Mutsumi Aihara Akiko Hirata Zehong Su Akira Takahashi VopB1 and VopD1 are essential for translocation of type III secretion system 1 effectors of Vibrio parahaemolyticus. Vibrio parahaemolyticus is a pathogenic Vibrio species that causes food-borne acute gastroenteritis, often related to the consumption of raw or undercooked seafood. Vibrio parahaemolyticus has 2 type III secretion systems (T3SS1 and T3SS2). Here, we demonstrate that VP1657 (VopB1) and VP1656 (VopD1), which share sequence similarity with Pseudomonas genes popB (38%) and popD (36%), respectively, are essential for translocation of T3SS1 effectors into host cells. A VP1680CyaA fusion reporter system was constructed to observe effector translocation. Using this reporter assay we showed that the VopB1 and VopD1 deletion strains were unable to translocate VP1680 to host cell but that the secretion of VP1680 into the culture medium was not affected. VopB1 or VopD1 deletion strains did not enhance cytotoxicity and failed to activate mitogen-activated protein kinases and secretion of interleukin-8, which depend on VP1680. Thus, we conclude that VopB1 and VopD1 are essential components of the translocon. To target VopB1 and VopD1 may have therapeutic potential for the treatment or prevention in V. parahaemolyticus infection. Canadian Journal of Microbiology 1480-3275 58 8 1002 1007 20120724 10.1139/w2012-081 22827847 6339428/published_papers/5037231 Masayuki Nakano Eiki Yamasaki Akitoyo Ichinose Takaaki Shimohata Akira Takahashi Junko K. Akada Kazuyuki Nakamura Joel Moss Toshiya Hirayama Hisao Kurazono Salmonella enterotoxin (Stn) regulates membrane composition and integrity DMM Disease Models and Mechanisms 1754-8403 5 4 515 521 20120700 10.1242/dmm.009324 22301710 2-s2.0-84863450177 6339428/published_papers/5037230 Tuyet Le Thi Nhung Hirofumi Nagata Akira Takahashi Mutsumi Aihara Toshihiro Okamoto Takaaki Shimohata Kazuaki Mawatari Masatake Akutagawa Yohsuke Kinouchi Masanobu Haraguchi Sterilization effect of UV light on Bacillus spores using TiO(2) films depends on wavelength. UV light and photocatalysts such as titanium dioxide (TiO(2)) and silver (Ag) are useful for disinfection of water and surfaces. However, the effect of UV wavelength on photocatalytic disinfection of spores is not well understood. Inactivation of Bacillus spores has been examined using different UV wavelengths and TiO(2) or TiO(2)/Ag composite materials. The level of UVA disinfection of Bacillus anthracis and Bacillus brevis vegetative cells increased with the presence of the TiO(2) and Ag photocatalysts, but had little effect on their spores. B. brevis spores were slightly more sensitive to UVB and UVC than the spores of B. atrophaeus. Photocatalytic sterilization against spores was strongest in UVC and UVB and weakest in UVA. The rate of inactivation of Bacillus spores was significantly increased by the presence of TiO(2), but was not markedly different from that induced by the presence of Ag. Therefore, TiO(2)/Ag plus UVA can be used for the sterilization of vegetative cells, while TiO(2) and UVC are effective against spores. The Journal of Medical Investigation : JMI 1349-6867 59 1-2 53 58 20120308 10.2152/jmi.59.53 22449993 6339428/published_papers/5037232 Nakagawa Tadahiko Nagakatsu Harada Miyamoto Aiko Kawanishi Yukiko Yoshida Masaki Masayuki Shono Kazuaki Mawatari Akira Takahashi Hiroshi Sakaue Yutaka Nakaya Membrane topology of murine glycerol-3-phosphate acyltransferase 2. Glycerol-3-phosphate acyltransferase (GPAT) is a rate-limiting enzyme in mammalian triacylglycerol biosynthesis. GPAT is a target for the treatment of metabolic disorders associated with high lipid accumulation. Although the molecular basis for GPAT1 activation has been investigated extensively, the activation of other isoforms, such as GPAT2, is less well understood. Here the membrane topology of the GPAT2 protein was examined using an epitope-tag-based method. Exogenously expressed GPAT2 protein was present in the membrane fraction of transformed HEK293 cells even in the presence of Na(2)CO(3) (100 mM), indicating that GPAT2 is a membrane-bound protein. Trypsin treatment of the membrane fraction degraded the N-terminal (FLAG) and C-terminal (myc-epitope) protein tags of the GPAT2 protein. Bioinformatic analysis of the GPAT2 protein sequence indicated four hydrophobic sequences as potential membrane-spanning regions (TM1-TM4). Immunoblotting of the myc-epitope tag, which was inserted between each TM region of the GPAT2 protein, showed that the amino acid sequence between TM3 and TM4 was protected from trypsin digestion. These results suggest that the GPAT2 protein has two transmembrane segments and that the N-terminal and C-terminal regions of this protein face the cytoplasm. These results also suggest that the enzymatically active motifs I-III of the GPAT2 protein face the cytosol, while motif IV is within the membrane. It is expected that the use of this topological model of GPAT2 will be essential in efforts to elucidate the molecular mechanisms of GPAT2 activity in mammalian cells. Biochemical and Biophysical Research Communications 1090-2104 418 3 506 511 20120120 10.1016/j.bbrc.2012.01.055 22285183 6339428/published_papers/5037233 Sonoko Yasui Kazuaki Mawatari Ran Morizumi Hiroko Furukawa Takaaki Shimohata Nagakatsu Harada Akira Takahashi Yutaka Nakaya Hydrogen peroxide inhibits insulin-induced ATP-sensitive potassium channel activation independent of insulin signaling pathway in cultured vascular smooth muscle cells. Both reactive oxygen species (ROS) and insulin resistance have been reported to play essential pathophysiological roles in cardiovascular diseases, such as hypertension and atherosclerosis. However, the mechanistic link between ROS and insulin resistance in the vasculature remains unclear. Recently we have shown that insulin causes membrane hyperpolarization via ATP-sensitive potassium (K(ATP)) channel activation, which is mediated by phosphatidylinositol 3-kinase (PI3-K) in cultured vascular smooth muscle cells (VSMCs). K(ATP) channel in the vasculature is critical in the regulation of vascular tonus. Here we examined the effects of ROS induced by hydrogen peroxide (H(2)O(2)) on insulin-induced K(ATP) channel activities in cultured VSMCs, A10 cells. H(2)O(2) (10 μM) increased significantly intercellular ROS in A10 cells. By using a cell-attached patch clamp experiment, 10 μM H(2)O(2) suppressed significantly insulin-induced K(ATP) channel activation without inhibition of insulin receptor signal transduction component including IRS and Akt in A10 cells. Furthermore 10 μM H(2)O(2) suppressed significantly pinacidil-induced K(ATP) channel activation in A10 cells. These data suggest that H(2)O(2) might inhibit directly K(ATP) channel independent of insulin signaling pathway. This study may contribute to our understanding of mechanisms of insulin resistance-associated cardiovascular disease. The Journal of Medical Investigation : JMI 1349-6867 59 1-2 36 44 20120000 10.2152/jmi.59.36 22449991 6339428/published_papers/5037234 H. Nazari A. Khaleghian A. Takahashi N. Harada N. J.G. Webster M. Nakano K. Kishi Y. Ebina Y. Nakaya Cortactin, an actin binding protein, regulates GLUT4 translocation via actin filament remodeling Biochemistry (Moscow) 0006-2979 76 11 1262 1269 20111100 10.1134/S0006297911110083 22117553 2-s2.0-82455205856 6339428/published_papers/5037235 Shinji Kawahito Takashi Kawano Hiroshi Kitahata Jun Oto Akira Takahashi Kazumi Takaishi Nagakatsu Harada Tadahiko Nakagawa Hiroyuki Kinoshita Toshiharu Azma Yutaka Nakaya Shuzo Oshita Molecular mechanisms of the inhibitory effects of clonidine on vascular adenosine triphosphate-sensitive potassium channels. We investigated the effects of the imidazoline-derived α2-adrenoceptor agonist clonidine on vascular adenosine triphosphate-sensitive potassium (K(ATP)) channel activity in rat vascular smooth muscle cells and recombinant vascular K(ATP) channels transiently expressed in COS-7 cells. Using the patch-clamp method, we investigated the effects of clonidine on the following: (1) native vascular K(ATP) channels; (2) recombinant K(ATP) channels with different combinations of various types of inwardly rectifying potassium channel (Kir6.0 family: Kir6.1, 6.2) and sulfonylurea receptor (SUR1, 2A, 2B) subunits; (3) SUR-deficient channels derived from a truncated isoform of the Kir6.2 subunit (Kir6.2ΔC36 channels); and (4) mutant Kir6.2ΔC36 channels with diminished sensitivity to ATP (Kir6.2ΔC36-K185Q channels). Clonidine (?3 × 10(-8) M) inhibited native K(ATP) channel activity in cell-attached configurations with a half-maximal inhibitory concentration value of 1.21 × 10(-6) M and in inside-out configurations with a half-maximal inhibitory concentration value of 0.89 × 10(-6) M. With similar potency, clonidine (10(-6) or 10(-3) M) also inhibited the activities of various recombinant SUR/Kir6.0 K(ATP) channels, the Kir6.2ΔC36 channel, and the Kir6.2ΔC36-K185Q channel. Clinically relevant concentrations of clonidine inhibit K(ATP) channel activity in vascular smooth muscle cells. This inhibition seems to be the result of its effect on the Kir6.0 subunit and not on the SUR subunit. Anesthesia & Analgesia 1526-7598 113 6 1374 1380 20111014 10.1213/ANE.0b013e3182321142 22003223 6339428/published_papers/5037236 Tuyet Nhung Thi Le Hirofumi Nagata Mutsumi Aihara Akira Takahashi Toshihiro Okamoto Takaaki Shimohata Kazuaki Mawatari Yhosuke Kinouchi Masatake Akutagawa Masanobu Haraguchi Additional effects of silver nanoparticles on bactericidal efficiency depend on calcination temperature and dip-coating speed. There is an increasing interest in the application of photocatalytic properties for disinfection of surfaces, air, and water. Titanium dioxide is widely used as a photocatalyst, and the addition of silver reportedly enhances its bactericidal action. However, the synergy of silver nanoparticles and TiO(2) is not well understood. The photocatalytic elimination of Bacillus atrophaeus was examined under different calcination temperatures, dip-coating speeds, and ratios of TiO(2), SiO(2), and Ag to identify optimal production conditions for the production of TiO(2)- and/or TiO(2)/Ag-coated glass for surface disinfection. Photocatalytic disinfection of pure TiO(2) or TiO(2) plus Ag nanoparticles was dependent primarily on the calcination temperature. The antibacterial activity of TiO(2) films was optimal with a high dip-coating speed and high calcination temperature (600°C). Maximal bacterial inactivation using TiO(2)/Ag-coated glass was also observed following high-speed dip coating but with a low calcination temperature (250°C). Scanning electron microscopy (SEM) showed that the Ag nanoparticles combined together at a high calcination temperature, leading to decreased antibacterial activity of TiO(2)/Ag films due to a smaller surface area of Ag nanoparticles. The presence of Ag enhanced the photocatalytic inactivation rate of TiO(2), producing a more pronounced effect with increasing levels of catalyst loading. Applied and Environmental Microbiology 1098-5336 77 16 5629 5634 20110701 10.1128/AEM.00049-11 21724887 6339428/published_papers/5037237 Hiroko Furukawa Kazuaki Mawatari Kei Koyama Sonoko Yasui Ran Morizumi Takaaki Shimohata Nagakatsu Harada Akira Takahashi Yutaka Nakaya Telmisartan increases localization of glucose transporter 4 to the plasma membrane and increases glucose uptake via peroxisome proliferator-activated receptor γ in 3T3-L1 adipocytes. European Journal of Pharmacology 1879-0712 660 2-3 485 491 20110600 10.1016/j.ejphar.2011.04.008 21514293 2-s2.0-79956145321 6339428/published_papers/5037238 Takaaki Shimohata Masayuki Nakano Xin Lian Tomomi Shigeyama Hitomi Iba Akiko Hamamoto Masaki Yoshida Nagakatsu Harada Hironori Yamamoto Masayuki Yamato Kazuaki Mawatari Toshiaki Tamaki Yutaka Nakaya Akira Takahashi Vibrio parahaemolyticus infection induces modulation of IL-8 secretion through dual pathway via VP1680 in Caco-2 cells. Vibrio parahaemolyticus causes acute gastroenteritis and inflammations in humans. A variety of pathogenic bacteria can stimulate mitogen-activated protein kinases (MAPKs) in host cells. Phosphorylation of MAPKs leads to production of interleukin (IL)- 8 and subsequently causes inflammations. Thus, MAPK cascades were strong candidates for the main signaling pathway of V. parahaemolyticus-induced acute inflammation. The Journal of Infectious Diseases 1537-6613 203 4 537 544 20110200 10.1093/infdis/jiq070 21177635 6339428/published_papers/5037239 Akiko Hamamoto Chiyo Bandou Masayuki Nakano Kazuaki Mawatari Xin Lian Masayuki Yamato Nagakatsu Harada Masatake Akutagawa Yohsuke Kinouchi Yutaka Nakaya Akira Takahashi Differences in stress response after UVC or UVA irradiation in Vibrio parahaemolyticus ENVIRONMENTAL MICROBIOLOGY REPORTS 1758-2229 2 5 660 666 20101000 10.1111/j.1758-2229.2010.00154.x 23766253 6339428/published_papers/5037241 Takashi Kawano Katsuya Tanaka hua Yin Satoru Eguchi Hiroaki Kawano Akira Takahashi Yutaka Nakaya Shuzo Oshita Effects of ketamine on nicorandil induced ATP-sensitive potassium channel activity in cell line derived from rat aortic smooth muscle. Nicorandil opens adenosine triphosphate-sensitive potassium (K(ATP)) channels in the cardiovascular system and is being increasingly used for the treatment of angina pectoris. In the present study, we tested whether intravenous anesthetic agent ketamine affected nicorandil-induced native vascular K(ATP) channel activation. The Journal of Medical Investigation : JMI 1349-6867 57 3-4 237 244 20100800 10.2152/jmi.57.237 20847523 6339428/published_papers/5037240 Takaaki Shimohata Akira Takahashi Diarrhea induced by infection of Vibrio parahaemolyticus. Vibrio parahaemolyticus is a human pathogen that naturally inhabits marine and estuarine environments. Infection with V. parahaemolyticus is often associated with the consumption of raw or undercooked seafood, causing gastroenteritis with watery diarrhea. The presence of two type III secretion system (T3SS) proteins, thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH), has been closely associated with the severity of diarrheal illness. TDH and TRH have various biological activities including hemolytic activity, cardiotoxicity, and enterotoxicity. T3SS1 is involved in cytotoxicity to host cells and orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, and cell lysis. T3SS2 is thought to be related to the enterotoxicity of V. parahaemolyticus. The activities of inducing diarrhea of each of the virulence factors were summarized in this review. The Journal of Medical Investigation : JMI 1349-6867 57 3-4 179 182 20100800 10.2152/jmi.57.179 20847516 6339428/published_papers/5037242 Ali Khaleghian Gholam Hossein Riazi Mahmoud Ghafari Marzieh Rezaie Akira Takahashi Yutaka Nakaya Hossain Nazari EFFECT OF INGANEN ANTICANCER PROPERTIES ON MICROTOBULE ORGANIZATION PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES 1011-601X 23 3 273 278 20100700 20566439 6339428/published_papers/5037243 Zehong Su Masayuki Nakano Tetsuro Koga Xin Lian Akiko Hamamoto Takaaki Shimohata Yumi Harada Kazuaki Mawatari Nagakatsu Harada Masatake Akutagawa Yutaka Nakaya Akira Takahashi Hfq regulates anti-oxidative ability in Vibrio parahaemolyticus JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY 1349-8037 56 3 181 186 20100600 10.2323/jgam.56.181 20647674 6339428/published_papers/5037244 Xin Lian Kayo Tetsutani Mai Katayama Masayuki Nakano Kazuaki Mawatari Nagakatsu Harada Akiko Hamamoto Masayuki Yamato Masatake Akutagawa Yohsuke Kinouchi Yutaka Nakaya Akira Takahashi A New Colored Beverage Disinfection System Using UV-A Light-Emitting Diodes BIOCONTROL SCIENCE 1884-0205 15 1 33 37 20100300 10.4265/bio.15.33 20361521 6339428/published_papers/5037245 Masaki Yoshida Nagakatsu Harada Keiko Yoshida Tadahiko Nakagawa Takaaki Shimohata Kazuaki Mawatari Akira Takahashi Hiroshi Sakaue Yutaka Nakaya High density lipoprotein inhibits the activation of sterol regulatory element-binding protein-1 in cultured cells. A link between cellular uptake of high density lipoprotein (HDL) and regulation of sterol regulatory element-binding protein-1 (SREBP-1) was investigated in vitro. HDL decreased nuclear SREBP-1 levels as well as SREBP-1 target gene expression in HepG2 and HEK293 cells. However, HDL did not repress an exogenously expressed, constitutively active form of SREBP-1. HDL increased cellular cholesterol levels, and cellular cholesterol depletion by methyl-beta-cyclodextrin abolished the effects of HDL. These results suggest that HDL inhibits the activation of SREBP-1 through a cholesterol-dependent mechanism, which may play an important role in regulating lipid synthetic pathways mediated by SREBP-1. FEBS Letters 1873-3468 584 6 1217 1222 20100218 10.1016/j.febslet.2010.02.034 20171215 6339428/published_papers/5037246 Hattori Atsushi Kazuaki Mawatari Tuzuki Satomi Yoshioka Emiko Toda Satomi Yoshida Masaki Yasui Sonoko Furukawa Hiroko Morishima Masaki Ono Katsushige Takamasa Ohnishi Nakano Masayuki Nagakatsu Harada Akira Takahashi Yutaka Nakaya β-Adrenergic-AMPK Pathway Phosphorylates Acetyl-CoA Carboxylase in a High-epinephrine Rat Model, SPORTS We established a new animal model called SPORTS (Spontaneously-Running Tokushima-Shikoku) rats, which show high-epinephrine (Epi) levels. Recent reports show that Epi activates adenosine monophosphate (AMP)-activated protein kinase (AMPK) in adipocytes. Acetyl-CoA carboxylase (ACC) is the rate-limiting enzyme in fatty acid synthesis, and the enzymatic activity is suppressed when its Ser-79 is phosphorylated by AMPK. The aim of this study was to investigate the in vivo effect of Epi on ACC and abdominal visceral fat accumulation. We divided both 6-week male control and SPORTS rats into two groups, which were fed either normal diet or high fat and sucrose (HFS) diet for 16 weeks. At the end of diet treatment, retroperitoneal fat was collected for western blotting and histological analysis. Food intake was not different among the groups, but SPORTS rats showed significantly lower weight gain than control rats in both diet groups. After 10 weeks of diet treatment, glucose tolerance tests (GTTs) revealed that SPORTS rats had increased insulin sensitivity. Furthermore, SPORTS rats had lower quantities of both abdominal fat and plasma triglyceride (TG). In abdominal fat, elevated ACC Ser-79 phosphorylation was observed in SPORTS rats and suppressed by an antagonist of beta-adrenergic receptor (AR), propranolol, or an inhibitor of AMPK, Compound C. From these results, high level of Epi induced ACC phosphorylation mediated through beta-AR and AMPK signaling pathways in abdominal visceral fat of SPORTS rats, which may contribute to reduce abdominal visceral fat accumulation and increase insulin sensitivity. Our results suggest that beta-AR-regulated ACC activity would be a target for treating lifestyle-related diseases, such as obesity. Obesity 1930-739X 18 1 48 54 20100100 10.1038/oby.2009.145 19444233 6339428/published_papers/5037248 hua Yin Nagakatsu Harada Kazuaki Mawatari Yasui Sonoko Hiroko Segawa Akira Takahashi Shuzo Oshita Yutaka Nakaya L-DOPA inhibits nitric oxide-dependent vasorelaxation via production of reactive ozygen species in rat aorta To clarify the underlying mechanisms of L-DOPA induced vasoconstriction in rat aorta. Methods: The effect of L-DOPA on phenylephrine-induced contractile force of blood vessels was examined in vitro using rat aortic ring preparations by isometric tension experiment. Involvement of nitric oxide (NO) in the effect of L-DOPA on vascular smooth muscle was studied by using N(omega)-Nitro-L-arginine (L-NNA), Sodium nitroprusside (SNP) in endothelium-intact and endothelium-denuded aortic rings. L-DOPA potentiated alpha-adrenergic receptor- and depolarization-induced vascular contraction and inhibited acetylcholine-induced vasorelaxation. This effect was diminished by pretreatment of the aortic rings with L-NNA, an inhibitor of NO synthesis, or by removing the endothelium from the ring preparations. In endothelium-denuded rings, L-DOPA inhibited exogenous NO-dependent but not cGMP-mediated vasorelaxation. Increases in cGMP levels in response to an NO donor were attenuated by L-DOPA in cultured rat aortic smooth muscle cells. L-DOPA could not contract rings (without endothelium) pretreated with 3-(5&#039;-hydroxymethyl- 2&#039;-furyl)-1-benzyl indazole (YC-1), an activator of guanylyl cyclase, but SOD (150 U/ml) pretreatment of rings with endothelium inhibited contraction by L-DOPA. These results suggest that L-DOPA inhibits nitric-dependent vasorelaxation on vascular smooth muscle cells via production of reactive oxygen species. The Journal of Medical Investigation : JMI 1349-6867 56 3,4 120 129 20090800 10.2152/jmi.56.120 19763024 6339428/published_papers/5037247 Gadelmoula Mostafa Lian Xin Maeda Miku Aihara Mutsumi Kazuaki Mawatari Hamamoto Akiko Harada Yumi Masayuki Yamato Masatake Akutagawa Yutaka Nakaya Yohsuke Kinouchi Akira Takahashi Suitability of ultraviolet(A)-light emitting diode for air stream disinfection We previously developed a high powered light-emitting diode device capable of discharging germicidal ultraviolet irradiation (UVA-LED) at an approximate wavelength of 365 nm. This study examined the bactericidal activity of UVA-LED in moving air streams. Aerosols of Escherichia coli DH5alpha were exposed to UVA-LED irradiation using a stable current (0.5 A and 1.2 mW/cm(2)) or pulse current (1.0 A and 0.2 mW/cm(2)). Settle plate analysis was used for bioaerosol sampling, where results were expressed as Colony Forming Units. A -3 Log inactivation of the E. coli population occurred after 75 minutes of constant exposure to stable current. The pulse current produced inactivation within a similar timeframe. Our results might be significant as a basic study for further investigations about the effect of UVA-LED on airborne bacteria and its suitability for air disinfection applications. The Journal of Medical Investigation : JMI 1349-6867 56 3-4 150 156 20090800 10.2152/jmi.56.150 19763028 6339428/published_papers/5037249 Masaki Yoshida Nagakatsu Harada Hironori Yamamoto Yutaka Taketani Tadahiko Nakagawa Yunjie Yin Atsushi Hattori Tomoe Zenitani Sayuri Hara Haruka Yonemoto Aki Nakamura Masayuki Nakano Kazuaki Mawatari Kiyoshi Teshigawara Hidekazu Arai Toshio Hosaka Aldra Takahashi Katsuhiko Yoshimoto Yutaka Nakaya Identification of cis-acting promoter sequences required for expression of the glycerol-3-phosphate acyltransferase 1 gene in mice BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 0006-3002 1791 1 39 52 20090100 10.1016/j.bbalip.2008.09.005 18983939 6339428/published_papers/5037250 Masayuki Nakano Akira Takahashi Zehong Su Nagakatsu Harada Kazuaki Mawatari Yutaka Nakaya Hfq regulates the expression of the thermostable direct hemolysin gene in Vibrio parahaemolyticus BMC MICROBIOLOGY 1471-2180 8 155 155 20080900 10.1186/1471-2180-8-155 18803872 6339428/published_papers/5037251 Takashi Kawano Katsuya Tanaka Kazuaki Mawatari Shuzo Oshita Akira Takahashi Yutaka Nakaya Hyperglycemia impairs isoflurane-induced adenosine triphosphate-sensitive potassium channel activation in vascular smooth muscle cells Isoflurane activates vascular adenosine triphosphate sensitive potassium (K(ATP)) channels, and may induce vasodilation. In the present study, we investigated whether hyperglycemia modifies isoflurane activation of vascular K(ATP) channel. We used a cell-attached patch-clamp configuration to test the effects of isoflurane on K(ATP) channel activity in vascular smooth muscle cells (VSMCs) after incubation for 24 h in medium containing normal glucose (NG, 5.5 mM D-glucose), L-glucose (LG, 5.5 mM D-glucose plus 17.5 mM L-glucose), or high glucose (HG, 23 mM D-glucose). Superoxide levels in aortas were measured by the lucigenin-enhanced chemiluminescence technique. Isoflurane-induced open probabilities were significantly reduced in VSMCs from arteries incubated in HG (0.06 +/- 0.01) compared with NG (0.17 +/- 0.02; P &lt; 0.05) and LG (0.15 +/- 0.02; P &lt; 0.05). Pretreatment of VSMCs with protein kinase C (PKC) inhibitors, calphostin C and PKC inhibitor 20-28, greatly reduced HG inhibition of isoflurane-induced K(ATP) channel activity. In addition, a PKC activator, PMA, mimicked the effects of HG. Superoxide release was significantly increased in arteries incubated in HG (18.3 +/- 11.5 relative light units (RLU) x s(-1) x mg(-1); P &lt; 0.05 versus NG). Coincubated with polyethylene glycol-superoxide dismutase (250 U/mL), a cell-permeable superoxide scavenger, greatly reduced the HG-induced increase of superoxide, but failed to reduce HG inhibition of isoflurane-induced K(ATP) channel activity. Our results suggest that the metabolic stress of hyperglycemia can impair isoflurane-induced vascular K(ATP) channel activity mediated by excessive activation of PKC. This could impede the coronary vasodilation response to isoflurane, causing ischemia or hypoxia in patients with perioperative hyperglycemia. Anesthesia & Analgesia 1526-7598 106 3 858 864 20080500 10.1213/ane.0b013e318163fd5b 18292430 6339428/published_papers/5037252 Nagakatsu Harada Haruka Yonemoto Masaki Yoshida Hironori Yamamoto Yunjie Yin Aiko Miyamoto Atsushi Hattori Qishisan Wu Tadahiko Nakagawa Masayuki Nakano Kiyoshi Teshigawara Kazuaki Mawatari Toshio Hosaka Akira Takahashi Yutaka Nakaya Alternative splicing produces a constitutively active form of human SREBP-1. Biochemical and Biophysical Research Communications 1090-2104 368 3 820 826 20080211 10.1016/j.bbrc.2008.02.004 18267114 6339428/published_papers/5037253 Hiroaki Kawano Takashi Kawano Hua Yin Katsuya Tanaka Satoru Eguchi Akira Takahashi Yutaka Nakaya Shuzo Oshita Effects of dopamine on ATP-sensitive potassium channels in porcine coronary artery smooth-muscle cells JOURNAL OF CARDIOVASCULAR PHARMACOLOGY 0160-2446 51 2 196 201 20080200 10.1097/FJC.0b013e31815f2be7 18287888 6339428/published_papers/5037254 Wu Qishisan Lian Xin Nakano Masayuki Hosaka Toshio Takahashi Akira Nakaya Yutaka Harada Nagakatsu Nakamura Aki Yoshida Masaki Mawatari Kazuaki Hattori Atsushi Li Qinkai Shimohata Takaaki Yinhua NO-1886, a lipoprotein lipase activator, attenuates contraction of rat intestinal ring preparations Various intestinal symptoms or diseases are closely associated with intestinal motility, which may be altered by metabolic disturbances associated with diabetes and obesity. It is therefore important that drugs used in the treatment of metabolic disorders should not have any adverse effects on the intestine. In the present study, we examined whether [4-(4-bromo-2-cyano-phenylcarbamoyl)-benzyl]-phosphonic acid diethyl ester (NO-1886), a lipoprotein lipase activator with anti-diabetic and/or anti-obese activity, affects stimulant-induced intestinal contractility. Administration of NO-1886 to intestinal ring preparations of ileum, rectum and colon isolated from Wistar rats attenuated or relaxed contraction induced by a high K+ environment or acetylcholine (ACh). This effect of NO-1886 was dependent on extracellular Ca<SUP>2+</SUP> and intracellular myosin light chain kinase activity. Our results also showed that ACh-induced colonic contraction was significantly higher in the obese Otsuka Long-Evans Tokushima Fatty (OLETF) than in the non-obese Long-Evans Tokushima Otsuka (LETO) rats. The hypercontractility observed in the colons of OLETF rats occurred concomitantly with an elevation in muscarinic M3 ACh receptor protein levels. Administration of NO-1886 attenuated the obesity-induced hypercontractility of the colonic rings of OLETF rats. Thus, intestinal contractile system would be a novel pharmacological target of the lipoprotein lipase activator NO-1886. J. Med. Invest. 55: 61-70, February, 2008 The University of Tokushima Faculty of Medicine 国立大学法人 徳島大学医学部 The Journal of Medical Investigation 1343-1420 55 1 61 70 20080000 10.2152/jmi.55.61 18319547 6339428/published_papers/5037257 A. Hamamoto M. Mori A. Takahashi M. Nakano N. Wakikawa M. Akutagawa T. Ikehara Y. Nakaya Y. Kinouchi New water disinfection system using UVA light-emitting diodes JOURNAL OF APPLIED MICROBIOLOGY 1364-5072 103 6 2291 2298 20071200 10.1111/j.1365-2672.2007.03464.x 18045413 6339428/published_papers/5037256 Mirei Mori Akiko Hamamoto Akira Takahashi Masayuki Nakano Noriko Wakikawa Satoko Tachibana Toshitaka Ikehara Yutaka Nakaya Masatake Akutagawa Yohsuke Kinouchi Development of a new water sterilization device with a 365 nm UV-LED MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING 0140-0118 45 12 1237 1241 20071200 10.1007/s11517-007-0263-1 17978842 6339428/published_papers/5037255 Satoru Eguchi Takashi Kawano Yinhua Katsuya Tanaka Sonoko Yasui Kazuaki Mawatari Akira Takahashi Yutaka Nakaya Shuzo Oshita Nobuyoshi Nakajo Effects of prostaglandin E-1 on vascular ATP-sensitive potassium channels JOURNAL OF CARDIOVASCULAR PHARMACOLOGY 1533-4023 50 6 686 691 20071200 10.1097/FJC.0b013e3181583d9b 18091586 6339428/published_papers/5037258 Hirohide Yamada Takashi Kawano Katsuya Tanaka Sonoko Yasui Kazuaki Mawatari Akira Takahashi Yutaka Nakaya Shuzo Oshita Effects of intracellular MgADP and acidification on the inhibition of cardiac sarcolemmal ATP-sensitive potassium channels by propofol JOURNAL OF ANESTHESIA 1438-8359 21 4 472 479 20071100 10.1007/s00540-007-0551-9 18008114 6339428/published_papers/5037260 Masori Maria Hamamoto Akiko Kazuaki Mawatari Nagakatsu Harada Akira Takahashi Yutaka Nakaya Angiotensin Decreases Glucose Uptake by Downregulation of GLUT1 in the Cell Membrene of the Vascular Smooth Muscule Cell Line A10 Recent evidence suggests a crosstalk between angiotensin II (Ang II) and insulin. However, whether this crosstalk affects glucose uptake, particularly in terms of actin filament involvement, has not yet been studied in vascular smooth muscle cells. Pretreatment of cells with either Ang II or cytochalasin D disarranged actin filaments in a time-dependent manner and inhibited glucose uptake. However, insulin increased actin reorganization and glucose uptake. Membrane fractionation studies showed that Ang II decreased GLUT-1 at the cell membrane, whereas it increased GLUT-1 in the cytoplasm, indicating that Ang II may cause internalization of GLUT-1 via actin disorganization, consequently decreasing glucose uptake. The effects of Ang II on glucose uptake and actin reorganization were blocked by AT1 receptor antagonist, but not by AT2 antagonist. Either P38 or ERK1/2 inhibitors partially reversed the Ang II-inhibited actin reorganization and glucose uptake, suggesting that MAPK signaling pathways could be involved as downstream events in Ang II signaling, and this signaling may interfere with insulin-induced actin reorganization and glucose uptake. These data imply that Ang II induces insulin resistance by decreasing glucose uptake via disarrangement of actin filaments, which provides a novel insight into understanding of insulin resistance by Ang II at the molecular level. Journal of Cardiovascular Pharmacology 0160-2446 50 3 267 273 20070900 10.1097/FJC.0b013e318093ec74 17878754 6339428/published_papers/5037259 Xin Lian Akira Takahashi Masayuki Nakano Emiko Hori Kazuaki Mawatari Nagakatsu Harada Toshio Hosaka Yutaka Nakaya Vibrio parahaemolyticus elevates interferon alpha production in intestinal-like epithelial Caco-2 cells CANADIAN JOURNAL OF MICROBIOLOGY 0008-4166 53 9 1084 1090 20070900 10.1139/W07-075 18026229 6339428/published_papers/5037261 Ono Kaori Kazuaki Mawatari Nagakatsu Harada Akira Takahashi Tohru Sakai Ogoshi Shohei Yutaka Nakaya Nucleoside and nucleotide mixture OG-VI rescues cytotoxicity induced by chemotherapeutic agents in intestinal-like epithelial cell. Immune cells and cells undergoing rapid turn-over can obtain exogenous nucleotides via salvage synthesis. We evaluated whether or not the balanced nucleoside and nucleotide mixture OG-VI, could rescue intestinal epithelial-like Caco-2 cells from the cytotoxic effects of several chemotherapeutic agents, in the presence and absence of glutamine (Gln). Cells were exposed to 5-fluorouracil (5FU), methotrexate (MTX) or 6-mercaptopurine (6MP), after which proliferation and cell cycle analyses were performed. Following exposure to the chemotherapeutic agents, we observed that cells treated with OG-VI proliferated well, whereas those without the supplement did not proliferate. Furthermore, following treatment with either 5FU or MTX, we observed that the number of cells in the G0/G1 phase decreased and those in the S phases increased. However, these cell cycle alterations were prevented by the addition of OG-VI. With the exception of 6MP-treated cells, we did not observe any effects on proliferation or cell cycle regulation that could be ascribed to the presence of Gln. Thus, we have demonstrated that OG-VI rescues cells from the cytotoxic effects of several chemotherapeutic agents. The Journal of Medical Investigation : JMI 1343-1420 54 3,4 235 242 20070800 10.2152/jmi.54.235 17878671 6339428/published_papers/5037266 Yan Wang Masaki Morishima Mingqi Zheng Tomoko Uchino Kazuaki Mannen Akira Takahashi Yutaka Nakaya Issei Komuro Katsushige Ono Transcription factors Csx/Nkx2.5 and GATA4 distinctly regulate expression of Ca2+ channels in neonatal rat heart JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY 1095-8584 42 6 1045 1053 20070600 10.1016/j.yjmcc.2007.03.905 17498735 6339428/published_papers/5037262 M. Nakano A. Takahashi Y. Sakai M. Kawano N. Harada K. Mawatari Y. Nakaya Catecholamine-induced stimulation of growth in Vibrio species LETTERS IN APPLIED MICROBIOLOGY 0266-8254 44 6 649 653 20070600 10.1111/j.1472-765X.2007.02136.x 17576228 6339428/published_papers/5037267 Masayuki Nakano Akira Takahashi Yuko Sakai Yutaka Nakaya Modulation of pathogenicity with norepinephrine related to the type III secretion system of Vibrio parahaemolyticus JOURNAL OF INFECTIOUS DISEASES 0022-1899 195 9 1353 1360 20070500 10.1086/513275 17397007 6339428/published_papers/5037264 Katsuya Tanaka Takashi Kawano Akiyo Nakamura Hossein Nazari Shinji Kawahito Shuzo Oshita Akira Takahashi Yutaka Nakaya Isoflurane activates sarcolemmal adenosine Triphosphate-sensitive potassium channels in vascular smooth muscle cells: A role of protein kinase A Recent evidence indicates that vascular adenosine triphosphate-sensitive potassium (K(ATP)) channels in vascular smooth muscle cells are critical in the regulation of vascular tonus under both physiologic and pathophysiologic conditions. Studies of the interaction of volatile anesthetics with vascular K(ATP) channels have been limited. In the current study, the authors investigated the molecular mechanism of isoflurane&#039;s action on vascular K(ATP) channels. Electrophysiologic experiments were performed using cell-attached and inside-out patch clamp techniques to monitor native vascular K(ATP) channels, and recombinant K(ATP) channels comprised of inwardly rectifying potassium channel subunits (Kir6.1) and the sulfonylurea receptor (SUR2B). Isometric tension experiments were performed in rat thoracic aortic rings without endothelium. Application of isoflurane (0.5 mM) to the bath solution during cell-attached recordings induced a significant increase in K(ATP) channel activity, which was greatly reduced by pretreatment with a selective inhibitor of protein kinase A (PKA), Rp-cAMPS (100 microM). In inside-out patches, isoflurane did not activate K(ATP) channels. Isoflurane significantly activated wild-type recombinant SUR2B/Kir6.1 in cell-attached patches. Isoflurane-induced activation of wild-type channels was diminished in the PKA-insensitive mutant SUR2B-T633A/Kir6.1, SUR2B-S1465A/Kir6.1, and SUR2B/Kir6.1-S385A. In addition, the authors demonstrated that isoflurane-induced PKA activation was associated with isoflurane-induced decreases in isometric tension in the rat aorta. These results indicate that isoflurane activates K(ATP) channels via PKA activation. PKA-dependent vasodilation induced by isoflurane also was observed in isometric tension experiments. Analysis of expressed vascular-type K(ATP) channels suggested that PKA-mediated phosphorylation of both Kir6.1 and SUR2B subunits plays a pivotal role in isoflurane-induced vascular K(ATP) channel activation. Anesthesiology 0003-3022 106 5 984 991 20070500 10.1097/01.anes.0000265158.47556.73 17457130 6339428/published_papers/5037265 Nagakatsu Harada Akiko Kusuyama Masaki Morishima Kazuko Okada Akira Takahashi Yutaka Nakaya Bezafibrate improves bacterial lipopolysaccharide-induced dyslipidemia and anorexia in rats METABOLISM-CLINICAL AND EXPERIMENTAL 0026-0495 56 4 517 522 20070400 10.1016/j.metabol.2006.11.011 17379010 6339428/published_papers/5037271 Akira Takahashi Shin-ichi Miyoshi Noriko Takata Masayuki Nakano Akiko Hamamoto Kazuaki Mawatari Nagakatsu Harada Sumio Shinoda Yutaka Nakaya Haemolysin produced by Vibrio mimicus activates two Cl- secretory pathways in cultured intestinal-like Caco-2 cells CELLULAR MICROBIOLOGY 1462-5822 9 3 583 595 20070300 10.1111/j.1462-5822.2006.00809.x 17026482 6339428/published_papers/5037269 Nagakatsu Harada Sayuri Hara Masaki Yoshida Tomoe Zenitani Kazuaki Mawatari Masayuki Nakano Akira Takahashi Toshio Hosaka Katsuhiko Yoshimoto Yutaka Nakaya Molecular cloning of a murine glycerol-3-phosphate acyltransferase-like protein 1 (xGPAT1) MOLECULAR AND CELLULAR BIOCHEMISTRY 0300-8177 297 1-2 41 51 20070300 10.1007/s11010-006-9321-5 17013544 6339428/published_papers/5037263 Akiyo Nakamura Shinji Kawahito Takashi Kawano Hossein Nazari Akira Takahashi Hiroshi Kitahata Yutaka Nakaya Shuzo Oshita Differential effects of etomidate and midazolam on vascular adenosine triphosphate-sensitive potassium channels: Isometric tension and patch clamp studies Anesthesiology 0003-3022 106 3 515 522 20070300 10.1097/00000542-200703000-00016 17325510 2-s2.0-34247463309 6339428/published_papers/5037270 Aki Nakamura Nagakatsu Harada Akira Takahashi Kazuaki Mawatari Masayuki Nakano Kazuhiko Tsutsumi Yutaka Nakaya NO-1886, a lipoprotein lipase activator, attenuates vascular smooth muscle contraction in rat aorta EUROPEAN JOURNAL OF PHARMACOLOGY 1879-0712 554 2-3 183 190 20070100 10.1016/j.ejphar.2006.09.059 17109854 6339428/published_papers/5037268 Nazari Hossein Takahashi Akira Harada Nagakatsu Mawatari Kazuaki Nakano Masayuki Kishi Kazuhiro Ebina Yousuke Nakaya Yutaka Angiotensin II inhibits insulin-induced actin stress fiber formation and glucose uptake via ERK1/2 There is crosstalk in intracellular signaling between Angiotensin II (Ang II) and insulin. We hypothesized that the underlying mechanism might be related to changes in cytoskeleton. In the presence of 100 nM of Ang II, insulin-induced glucose uptake was decreased and insulin-induced actin filament organization was inhibited. PKC inhibitors, including GF109203x and p38MAPK inhibitor (SB203580) neither improved insulin-induced actin reorganization nor glucose uptake. In contrast, the Ang II-induced inhibition of glucose uptake and actin filament disorganization was reversed by 10 &micro;mol ERK 1/2 MAPK inhibitor (PD98059). Pretreatment of Ang II increased ERK1/2 phosphorylation and inhibited insulin-induced Akt phosphorylation. The effect of Ang II on ERK1/2 phosphorylation was blocked by Ang II type 1 receptor antagonists, RNH6270 and PD98059 but not by SB203580 or Guanosine-5&rsquo;-O-(2-ThioDiphosphate), a G-protein inhibitor. We next tested the effect of broad-spectrum matrix metalloproteinase (MMP) inhibitor (GM6001) on Ang II-inhibition of insulin signaling pathway. GM6001 did not improve Ang II-induced actin filament disorganization and did not inhibit ERK1/2 phosphorylation. From these data in L6 myotube, we conclude that Ang II negatively regulates the insulin signal not through MMP signaling pathway but specifically through MMP-independent ERK1/2 activation pathway, providing an alternative molecular mechanism for angiotensin-induced insulin resistance. J. Med. Invest. 54: 19-27, February, 2007 The University of Tokushima Faculty of Medicine 国立大学法人 徳島大学医学部 The Journal of Medical Investigation 1343-1420 54 1 19 27 20070000 10.2152/jmi.54.19 17380010 6339428/published_papers/5037275 Masaki Morishima Nagakatsu Harada Sayuri Hara Atsuko Sano Hiromasa Seno Akira Takahashi Yusuke Morita Yutaka Nakaya Monoamine oxidase A activity and norepinephrine level in hippocampus determine hyperwheel running in SPORTS rats NEUROPSYCHOPHARMACOLOGY 1740-634X 31 12 2627 2638 20061200 10.1038/sj.npp.1301028 16421512 6339428/published_papers/5037272 Akira Takahashi Chiyo Yamamoto Toshio Kodama Kanami Yamashita Nagakatsu Harada Masayuki Nakano Takeshi Honda Yutaka Nakaya Pore formation of thermostable direct hemolysin secreted from Vibrio parahaemolyticus in lipid bilayers INTERNATIONAL JOURNAL OF TOXICOLOGY 1091-5818 25 5 409 418 20060900 10.1080/10915810600868181 16940013 6339428/published_papers/5037273 A Takahashi M Nakano K Okamoto Y Fujii K Mawatari N Harada Y Nakaya Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3- FEMS MICROBIOLOGY LETTERS 0378-1097 258 1 92 95 20060500 10.1111/j.1574-6968.2006.00204.x 16630261 6339428/published_papers/5037274 R Matsushima A Takahashi Y Nakaya H Maezawa M Miki Y Nakamura F Ohgushi S Yasuoka Human airway trypsin-like protease stimulates human bronchial fibroblast proliferation in a protease-activated receptor-2-dependent pathway AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY 1040-0605 290 2 L385 L395 20060200 10.1152/ajplung.00098.2005 16199437 6339428/published_papers/5037276 森島 真幸 原 小百合 Nagakatsu Harada 原田 永勝 Atsuko Sano 佐野 敦子 Hiromasa Seno 妹尾 廣正 Akira Takahashi 橋 章 Yutaka Nakaya 中屋 豊 高運動モデルラットSPORTSの海馬におけるノルエピネフリン動態と自発運動量 Shikoku Acta Medica 四国医学雑誌 0037-3699 61 5,6 185 188 20051200 6339428/published_papers/5037277 Yutaka Nakaya Yasuko Hata Kaori Ishida Akira Takahashi Kyoko Morita Kazuhito Rokutan Approach to novel functional foods for stress control 2. Microarray assessment of exercise in healthy volunteers Journal of Medical Investigation 1343-1420 52 Suppl 242 243 20051100 10.2152/jmi.52.242 16366507 2-s2.0-30144437409 6339428/published_papers/5037278 K Tsuchiya Kawamura, I A Takahashi T Nomura C Kohda M Mitsuyama Listeriolysin O-induced membrane permeation mediates persistent interleukin-6 production in Caco-2 cells during Listeria monocytogenes infection in vitro INFECTION AND IMMUNITY 0019-9567 73 7 3869 3877 20050700 10.1128/IAI.73.7.3869-3877.2005 15972472 6339428/published_papers/5037280 M Morishima-Yamato F Hisaoka S Shinomiya N Harada H Matoba A Takahashi Y Nakaya Cloning and establishment of a line of rats for high levels of voluntary wheel running LIFE SCIENCES 0024-3205 77 5 551 561 20050600 10.1016/j.lfs.2004.10.074 15904672 6339428/published_papers/5037279 A Takahashi N Tanoue M Nakano A Hamamoto K Okamoto Y Fujii N Harada Y Nakaya A pore-forming toxin produced by Aeromonas sobria activates Ca2+ dependent Cl- secretion MICROBIAL PATHOGENESIS 0882-4010 38 4 173 180 20050400 10.1016/j.micpath.2005.01.003 15797812 6339428/published_papers/5037302 Rie Matsushima Akira Takahashi Yutaka Nakaya Mari Miki Yoichi Nakamura Fumiko Michishige Sumiko Yoshinaga Hiroshi Maezawa Susumu Yasuoka Tripsin-like activities and human airway trypsin-like protease (HAT) levels in airway secretions from healthy subjects and patients with chronic airway diseases. Proceeding of Airway Secretion Research 7 15 22 20050301 6339428/published_papers/5037283 T Kawano S Oshita A Takahashi Y Tsutsumi K Tanaka Y Tomiyama H Kitahata Y Nakaya Molecular mechanisms underlying ketamine-mediated inhibition of sarcolemmal adenosine triphosphate-sensitive potassium channels ANESTHESIOLOGY 0003-3022 102 1 93 101 20050100 10.1097/00000542-200501000-00017 15618792 6339428/published_papers/5037282 A Wada AP Wang H Isomoto Y Satomi T Takao A Takahashi S Awata T Nomura Y Fujii S Kohno K Okamoto J Moss JL Millan T Hirayama Placental and intestinal alkaline phosphatases are receptors for Aeromonas sobria hemolysin INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY 1438-4221 294 7 427 435 20050100 10.1016/j.ijmm.2004.09.012 15715171 6339428/published_papers/5037281 N Tanoue A Takahashi K Okamoto Y Fujii Y Taketani N Harada M Nakano Y Nakaya A pore-forming toxin produced by Aeromonas sobria activates cAMP-dependent Cl- secretory pathways to cause diarrhea FEMS MICROBIOLOGY LETTERS 0378-1097 242 2 195 201 20050100 10.1016/j.femsle.2004.11.009 15621437 6339428/published_papers/5037285 T Kawano S Oshita A Takahashi Y Tsutsumi Y Tomiyama H Kitahata Y Kuroda Y Nakaya Molecular mechanisms of the inhibitory effects of bupivacaine, levobupivacaine, and ropivacaine on sarcolemmal adenosine triphosphate-sensitive potassium channels in the cardiovascular system ANESTHESIOLOGY 0003-3022 101 2 390 398 20040800 10.1097/00000542-200408000-00020 15277922 6339428/published_papers/5037284 K Mawatari S Kakui N Harada T Ohnishi Y Niwa K Okada A Takahashi K Izumi Y Nakaya Endothelin-1(1-31) levels are increased in atherosclerotic lesions of the thoracic aorta of hypercholesterolemic hamsters ATHEROSCLEROSIS 0021-9150 175 2 203 212 20040800 10.1016/j.atherosclerosis.2003.10.015 15262175 6339428/published_papers/5037286 Nagakatsu Harada Chika Ninomiya Yoshie Osako Masaki Morishima Kazuaki Mawatari Akira Takahashi Yutaka Nakaya Taurine alters respiratory gas exchange and nutrient metabolism in type 2 To assess the effect of taurine supplementation on respiratory gas exchange, which might reflect the improved metabolism of glucose and/or lipid in the type 2 diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Male OLETF rats (16 weeks of age) were randomly divided into two groups: unsupplemented group and taurine-supplemented (3% in drinking water) group. After 9 weeks of treatment, indirect calorimetry and insulin tolerance tests were conducted. The amounts of visceral fat pads, tissue glycogen, the blood concentrations of glucose, triacylglycerol, taurine, and electrolytes, and the level of hematocrit were compared between groups. A nondiabetic rat strain (Long-Evans Tokushima Otsuka) was used as the age-matched normal control. The indirect calorimetry showed that the treatment of OLETF rats with taurine could reduce a part of postprandial glucose oxidation possibly responsible for the increase of triacylglycerol synthesis in the body. Taurine supplementation also improved hyperglycemia and insulin resistance and increased muscle glycogen content in the OLETF rats. Supplementation with taurine increased the blood concentration of taurine and electrolyte and fluid volume, all of which were considered to be related to the improvement of metabolic disturbance in OLETF rats. Taurine supplementation may be an effective treatment for glucose intolerance and fat/lipid accumulation observed in type 2 diabetes associated with obesity. These metabolic changes might be ascribed, in part, to the alteration of circulating blood profiles, where the improved hyperglycemia and/or the blood accumulation of taurine itself would play roles. Obesity Research 1071-7323 12 7 1077 1084 20040700 10.1038/oby.2004.135 15292471 6339428/published_papers/5037287 T Kawano S Oshita A Takahashi Y Tsutsumi Y Tomiyama H Kitahata Y Kuroda Y Nakaya Molecular mechanisms of the inhibitory effects of propofol and thiamylal on sarcolemmal adenosine triphosphate-sensitive potassium channels ANESTHESIOLOGY 0003-3022 100 2 338 346 20040200 10.1097/00000542-200402000-00024 14739809 6339428/published_papers/5037288 S Kakui K Mawatari T Ohnishi Y Niwa N Tanoue N Harada A Takahashi K Izumi Y Nakaya Localization of the 31-amino-acid endothelin-1 in hamster tissue LIFE SCIENCES 0024-3205 74 11 1435 1443 20040100 10.1016/j.lfs.2003.08.021 14706574 6339428/published_papers/5037289 S Manabe Kuroda, I K Okada M Morishima M Okamoto N Harada A Takahashi K Sakai Y Nakaya Decreased blood levels of lactic acid and urinary excretion of 3-methylhistidine after exercise by chronic taurine treatment in rats JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY 0301-4800 49 6 375 380 20031200 10.3177/jnsv.49.375 14974726 6339428/published_papers/5037290 Mari Miki Yoichi Nakamura Akira Takahashi Yutaka Nakaya Hiroshi Eguchi Tsukio Masegi Kazuo Yoneda Susumu Yasuoka Saburo Sone Effect of human airway trypsin-like protease on intracellular free Ca2+ concentration in human bronchial epithelial cells. It has been shown that human airway trypsin-like protease (HAT) is localized in human bronchial epithelial cells (HBEC), and trypsin activates protease-activated receptor-2 (PAR-2). Activation of PAR-2 activates G-protein followed by an increase of intracellular free Ca2+, [Ca2+]in. This study was undertaken to clarify whether HAT can activate PAR-2 in HBEC or not. RT-PCR showed that HAT mRNA is expressed in HBEC, and PAR-2 mRNA is the most strongly expressed of the known PARs in HBEC. Both PAR-2 agonist peptide (PAR-2 AP) and HAT increased [Ca2+]in in HBEC in a biphasic fashion; a prompt, sharp increase (peak I) and a sustained low plateau (peak II). PAR-2 AP over 100-200 microM and HAT over 200-300 mU/ml (0.08-0.12 microM) induced both peak I and II, and PAR-2 AP below 100 microM and HAT below 200 mU/ml induced only peak II. Both PAR-2 AP-induced and HAT-induced peak I were induced by Ca2+ mobilization from intracellular stores, because they appeared even in Ca2+-free medium. Both PAR-2 AP-induced and HAT-induced peak II were induced by an influx of extracellular Ca2+, because they were abolished in Ca2+-free medium. The Ca2+ response to HAT was desensitized by exposure of HBEC to PAR-2 AP. These results indicate that HBEC have a functional PAR-2, and HAT regulates cellular functions of HBEC via activation of PAR-2. The journal of medical investigation : JMI 1343-1420 50 1-2 95 107 20030200 12630574 6339428/published_papers/5037291 Y Nakaya N Harada S Kakui K Okada A Takahashi J Inoi S Ito Severe catabolic state after prolonged fasting in cirrhotic patients: effect of oral branched-chain amino-acid-enriched nutrient mixture JOURNAL OF GASTROENTEROLOGY 0944-1174 37 7 531 536 20020700 10.1007/s005350200082 12162411 6339428/published_papers/5037293 T Kawano S Oshita Y Tsutsumi Y Tomiyama H Kitahata Y Kuroda A Takahashi Y Nakaya Clinically relevant concentrations of propofol have no effect on adenosine triphosphate-sensitive potassium channels in rat ventricular myocytes ANESTHESIOLOGY 0003-3022 96 6 1472 1477 20020600 10.1097/00000542-200206000-00029 12170062 6339428/published_papers/5037294 H Segawa Kaneko, I A Takahashi M Kuwahata M Ito Ohkido, I S Tatsumi K Miyamoto Growth-related renal type IINa/P-i cotransporter JOURNAL OF BIOLOGICAL CHEMISTRY 0021-9258 277 22 19665 19672 20020500 10.1074/jbc.M200943200 11880379 6339428/published_papers/5037292 Kinoshita Manabu Nakaya Yutaka Harada Nagakatsu Takahashi Akira Nomura Masahiro Bando Shigenobu Combination therapy of exercise and angiotensin-converting enzyme inhibitor markedly improves insulin sensitivities in hypertensive patients with insulin resistance Circulation Journal 1346-9843 66 7 655 658 20020000 10.1253/circj.66.655 12135133 2-s2.0-0036306907 6339428/published_papers/5037295 Akira Takahashi Wada Akihiro Ogushi Ken-ichi Maeda Kyoko Kawahara Tsukasa Kazuaki Mawatari Kurazono Hisao Moss Joel Hirayama Toshiya Yutaka Nakaya Production of L-defensin-2 by human colonic epithelial cells induced by Salmonella enteritidis flagella filament structural protein We recently showed that FliC of Salmonella enteritidis increased human beta-defensin-2 (hBD-2) expression, and now describe the signaling responsible pathway. FliC increased the intracellular Ca(2+) concentration ([Ca(2+)](in)) in Caco-2 cells. The [Ca(2+)](in) increase induced by FliC was prevented by U73122 and heparin, but not by chelating extracellular Ca(2+) or pertussis toxin. The FliC-induced increase in hBD-2 promoter activity via nuclear factor kappaB (NF-kappaB) was also inhibited by chelation of intracellular Ca(2+) or by U73122. We conclude that FliC increased [Ca(2+)](in) via inositol 1,4,5-trisphosphate, which was followed by up-regulating hBD-2 mRNA expression via an NF-kappaB-dependent pathway. FEBS Letters 0014-5793 508 3 484 488 20011123 10.1016/S0014-5793(01)03088-5 11728477 6339428/published_papers/5037296 A TAKAHASHI T IKEHARA K HOSOKAWA R OGURA H YAMAGUCHI Y NAKAYA H MIYAMOTO PROPERTIES OF CA2+ DEPENDENT K+ CHANNELS OF HUMAN GINGIVAL FIBROBLASTS JOURNAL OF DENTAL RESEARCH 0022-0345 74 8 1507 1512 19950800 6339428/published_papers/5037297 Zhen-Lin Jiang Hisao Yamaguchi Hiroyuki Tanaka Akira Takahashi Shingo Tanabe Noboru Utsuyama Toshitaka Ikehara Keiko Hosokawa Yohsuke Kinouchi Hiroshi Miyamoto Blood flow velocity in the commom carotid artery in humans during graded exercise on a treadmill Europian Journal of Physiology 70 234 239 19950000 6339428/published_papers/5037298 JF HE ZL JIANG H TANAKA T IKEHARA A TAKAHASHI H YAMAGUCHI H MIYAMOTO T IRITANI Y KINOUCHI CHANGES IN CAROTID BLOOD-FLOW AND ELECTROCARDIOGRAM IN HUMANS DURING AND AFTER WALKING ON A TREADMILL EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY AND OCCUPATIONAL PHYSIOLOGY 0301-5548 67 6 486 491 19931200 10.1007/BF00241643 8149926 6339428/published_papers/5037299 H YAMAGUCHI K HOSOKAWA ZL JIANG A TAKAHASHI T IKEHARA H MIYAMOTO ARREST OF CELL-CYCLE PROGRESSION OF HELA-CELLS IN THE EARLY G1 PHASE IN K+-DEPLETED CONDITIONS AND ITS RECOVERY UPON ADDITION OF INSULIN AND LDL JOURNAL OF CELLULAR BIOCHEMISTRY 0730-2312 53 1 13 20 19930900 6339428/published_papers/5037300 T IKEHARA H YAMAGUCHI K HOSOKAWA A TAKAHASHI H MIYAMOTO KINETIC-STUDY ON THE EFFECTS OF INTRACELLULAR K+ AND NA+ ON NA+,K+,CL- COTRANSPORT OF HELA-CELLS BY RB+ INFLUX DETERMINATION JOURNAL OF MEMBRANE BIOLOGY 0022-2631 132 2 115 124 19930300 6339428/misc/47952157 DUC Tran Quang UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira Unraveling the Capacity and Mechanism of Gut Microbiota in Enhancing Essential Amino Acid Provision to Juvenile Mice Under Essential Amino Acid-Deficient Diet 必須アミノ酸欠乏食下の幼若マウスへの必須アミノ酸供給増強における腸Microbiotaの能力と機構の解明【JST機械翻訳】||| 四国医学雑誌(Web) 2758-3279 80 1-2 null null 20240000 6339428/misc/47952070 馬渡一諭 上番増喬 下畑隆明 高橋章 馬渡一諭 野原一成 YOO Seung-Hee CHEN Zheng 小池宣也 八木田和弘 敷島康普 三浦宏之 新居佳孝 スダチ果皮特有のフラボノイド・スダチチンの概日リズム調節作用と肝脂質代謝改善作用 四国医学雑誌(Web) 2758-3279 79 5-6 null null 20240000 6339428/misc/47952163 MAKIMOTO Mana FUKUSHIMA Shiho YAMANAKA Saki SHIMOHATA Takaaki SHIMOHATA Takaaki UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira The effect of succinic acid on Campylobacter jejuni infection Campylobacter jejuni感染へのコハク酸の影響 日本細菌学雑誌(Web) 1882-4110 78 1 null null 20230000 6339428/misc/47952160 SHIMOHATA Takaaki SHIMOHATA Takaaki TSUJIGUCHI Mai FUKUSHIMA Shiho MAKIMOTO Mana KIDO Junko YOSHIMOTO Ayumi ISHIDA Kai UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira Effect of statin treatment on Campylobacter jejuni colonization in mouse infection model 感染モデルマウスを用いたCampylobacter jejuniの腸管定着に対するスタチン投与の影響 日本細菌学雑誌(Web) 1882-4110 78 1 null null 20230000 6339428/misc/47952105 森奈津 山本光生 富久章子 和田敬宏 岡本雅之 伊藤浩 粟飯原睦美 下畑隆明 鈴木浩司 安野卓 榎本崇宏 芥川正武 上番増喬 馬渡一諭 木内陽介 高橋章 UV-LEDを使った鶏舎光環境システムの開発 徳島県立農林水産総合技術支援センター畜産研究課研究報告 1881-2619 22 null null 20230000 6339428/misc/47952099 石田快 斧田優志 斧田優志 石川寧子 田中佐保 山下路代 福島志帆 相澤俊彦 山内繁晴 藤川康夫 田中智毅 上番増喬 馬渡一諭 高橋章 UV-LEDの光学特性に適した照射装置による細菌,真菌,ウイルスに対する波長依存的不活化効果の評価 日本防菌防黴学会年次大会要旨集 50th null null 20230000 6339428/misc/47952097 下畑隆明 下畑隆明 福島志帆 牧本真奈 上番増喬 馬渡一諭 高橋章 Campylobacter jejuniの生存戦略 日本防菌防黴学会年次大会要旨集 50th null null 20230000 6339428/misc/47952094 斧田優志 斧田優志 石田快 石川寧子 田中佐保 山下路代 福島志帆 相澤俊彦 山内繁晴 藤川康夫 田中智毅 上番増喬 馬渡一諭 高橋章 UV-LEDの光学特性に適したUV感受性評価のための標準化光源の開発 日本防菌防黴学会年次大会要旨集 50th null null 20230000 6339428/misc/47952091 白石志帆 白石志帆 下畑隆明 下畑隆明 上番増喬 馬渡一諭 高橋章 Lysosomes are Utilized to the Intracellular Survival of Campylobacter jejuni Campylobacter jejuniはリソソームを細胞内生存に利用する 日本分子生物学会年会プログラム・要旨集(Web) 46th null null 20230000 6339428/misc/47952087 射場仁美 下畑隆明 上番増喬 馬渡一諭 高橋章 腸炎ビブリオの病原因子T3SS1の遺伝子発現誘導条件の検討 日本食品微生物学会学術総会講演要旨集 44th null null 20230000 6339428/misc/47952083 牧本真奈 福島志帆 山中咲季 下畑隆明 下畑隆明 上番増喬 馬渡一諭 高橋章 Campylobacter jejuni病原性に対するコハク酸の影響 日本食品微生物学会学術総会講演要旨集 44th null null 20230000 6339428/misc/47952080 下畑隆明 下畑隆明 石田快 畑山翔 畑山翔 福島志帆 牧本真奈 木戸純子 上番増喬 馬渡一諭 高橋章 コレステロール分布がCampylobacter jejuni上皮細胞への感染に与える影響 日本生化学会大会(Web) 96th null null 20230000 6339428/misc/47952073 戸田沙慧 馬渡一諭 殿脇壱成 平野希美 山口ももか BUI Thi Kim Ngan 石川寧子 篠田浩一 上番増喬 高橋章 柑橘由来ポリメトキシフラボンは新型コロナウイルスの宿主細胞内複製を抑制する 日本栄養・食糧学会中国・四国支部大会講演要旨集(CD-ROM) 56th null null 20230000 6339428/misc/47952170 MAKIMOTO Mana FUKUSHIMA Shiho YAMANAKA Saki SHIMOHATA Takaaki SHIMOHATA Takaaki UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira The effect of organic acid on Campylobacter jejuni infection 有機酸によるCampylobacter jejuni感染への影響 日本細菌学雑誌(Web) 1882-4110 77 1 null null 20220000 6339428/misc/47952167 FUKUSHIMA Shiho SHIMOHATA Takaaki SHIMOHATA Takaaki UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira LC3 is utilized to the invasion of Campylobacter jejuni via Rac1 signaling pathway Campylobacter jejuniは,Rac1シグナル伝達を介してLC3を細胞内侵入に利用する 日本細菌学雑誌(Web) 1882-4110 77 1 null null 20220000 6339428/misc/47952117 石田快 粟飯原睦美 下畑隆明 北山栞 勢川怜花 上番増喬 馬渡一諭 鈴木浩司 榎本崇宏 芥川正武 山本光生 富久章子 和田敬宏 岡本雅之 伊藤浩 安野卓 木内陽介 高橋章 鶏舎内へのUV-LED導入による鶏の生育および衛生環境の改善効果の検討 日本家禽学会誌 0029-0254 59 null null 20220000 6339428/misc/47952111 石田快 粟飯原睦美 下畑隆明 北山栞 勢川怜花 上番増喬 馬渡一諭 鈴木浩司 榎本崇宏 芥川正武 山本光生 富久章子 和田敬宏 岡本雅之 伊藤浩 安野卓 木内陽介 高橋章 鶏舎におけるUV-LED導入による衛生環境改善効果の検討 日本食品微生物学会学術総会講演要旨集 43rd null null 20220000 6339428/misc/47952173 FUKUSHIMA Shiho SHIMOHATA Takaaki UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira Campylobacter jejuni recruit LC3 to bacterial invasion site on host cells through Rac1 signaling Campylobacter jejuniは,感染のために宿主細胞の侵入部位へとLC3を動員する 日本細菌学雑誌(Web) 1882-4110 76 1 null null 20210000 6339428/misc/47952184 ISHIDA Kai SHIMOHATA Takaaki KANDA Yuna MASUDA Rumiko UEBANSO Takashi MAWATARI Kazuaki KASHIMOTO Takashige TAKAHASHI Akira Metabolism Changing of Vibrio vulnificus Infected Tissue in Wound Infection Model Mouse 創傷感染モデルマウスにおけるVibrio vulnificus感染組織の代謝変化 日本細菌学雑誌(Web) 1882-4110 75 1 null null 20200000 6339428/misc/47952177 ISHIDA Kai SHIMOHATA Takaaki KANDA Yuna MASUDA Rumiko UEBANSO Takashi MAWATARI Kazuaki KASHIMOTO Takashige TAKAHASHI Akira Metabolism Changing of Vibrio vulnificus Infected Tissue in Wound Infection Model Mouse 創傷感染症モデルマウスにおけるVibrio vulnificus感染組織の代謝変化 日本細菌学雑誌(Web) 1882-4110 75 1 null null 20200000 6339428/misc/47952190 FUKUSHIMA Shiho SHIMOHATA Takaaki KIDO Junko ISHIDA Kai UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira Host Autophagy was Exploited to Campylobacter jejuni Invasion in the Epithelial Cells オートファジーの誘導はCampylobacter jejuniの宿主細胞への侵入過程を促進する 日本細菌学雑誌(Web) 1882-4110 74 1 null null 20190000 6339428/misc/47952187 ISHIDA Kai SHIMOHATA Takaaki HATAYAMA Syou KIDO Junko KANDA Yuna TENTAKU Aya FUKUSHIMA Shiho UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira Effect of cholesterol on Campylobacter jejuni survival in host intestinal epithelial cells 宿主腸上皮細胞におけるCampylobacter jejuniの生存に対するコレステロールの影響 日本細菌学雑誌(Web) 1882-4110 74 1 null null 20190000 6339428/misc/47952180 ULFA Maria SHIMOHATA Takaaki FUKUSHIMA Shiho AZUMA Momoyo UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira The Effectiveness of UVA-LED Irradiation on ESBL Producing Escherichia coli ESBL産生大腸菌に対するUVA-LED照射の効果 日本細菌学雑誌(Web) 1882-4110 74 1 null null 20190000 6339428/misc/47952236 Akari Tsunedomi Katsuyuki Miyawaki Akinori Masamura Mutsumi Nakahashi Kazuaki Mawatari Takaaki Shimohata Takashi Uebanso Yousuke Kinouchi Masatake Akutagawa Takahiro Emoto Akira Takahashi UVA-LED device to disinfect hydroponic nutrient solution 水耕栄養液を消毒するUVA-LEDデバイス Journal of Medical Investigation (Web) 1349-6867 65 3.4 null null 20180000 6339428/misc/47952234 ULFA Maria SHIMOHATA Takaaki FUKUSHIMA Shiho UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira AZUMA Momoyo Application of UVA-LED on Extended-Spectrum β-Lactamase (ESBL) Producing Escherichia coli from Clinical Isolates 臨床分離株由来の基質特異性拡張型βラクタマーゼ(ESBL)産生大腸菌に対するUVA-LEDの応用 四国医学雑誌 0037-3699 74 5-6 null null 20180000 6339428/misc/47952202 QUOC Anh Nguyen SHIMOHATA Takaaki HATAYAMA Sho TENTAKU Aya KIDO Junko UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira Metabolomic analysis of epithelial cellular metabolism during Vibrio parahaemolyticus infection Vibrio parahaemolyticus感染時の上皮細胞代謝のメタボロミクス解析 日本細菌学雑誌(Web) 1882-4110 73 1 null null 20180000 6339428/misc/47952198 TENTAKU Aya SHIMOHATA Takaaki HATAYAMA Sho QUOC Anh Nguyen KIDO Junko FUKUSHIMA Shiho UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira ER stress induced by Campylobacter jejuni-Infection Suppresses the Bacterial Invasion Campylobacter jejuni感染における小胞体ストレス応答は菌の侵入を抑制する 日本細菌学雑誌(Web) 1882-4110 73 1 null null 20180000 6339428/misc/47952194 FUKUSHIMA Shiho SHIMOHATA Takaaki HATAYAMA Sho KIDO Junko TENTAKU Aya KANDA Yuna UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira Campylobacter jejuni Utilized Autophagy for Survival and Invasion of the Bacteria in the Host Cells Campylobacter jejuniは菌の宿主上皮細胞への侵入および生存過程にオートファジーを利用する 日本細菌学雑誌(Web) 1882-4110 73 1 null null 20180000 6339428/misc/47952217 SHIMOHATA Takaaki KIDO Junko SATO Yuri HATAYAMA Sho KANDA Yuna FUKUSHIMA Shiho TENTAKU Aya UEBANSO Takashi MAWATARI Kazuaki TAKAHASHI Akira Campylobacter jejuni infection activates amino acid uptake in HeLa cell Campylobacter jejuni感染はHeLa細胞におけるアミノ酸取り込みを活性化する 日本細菌学雑誌(Web) 1882-4110 72 1 null null 20170000 6339428/misc/47952213 UEBANSO Takashi YOSHIMOTO Ayumi SHIMOHATA Takaaki NAKAHASHI Mutsumi MAWATARI Kazuaki TAKAHASHI Akira Does metabolites in gut world reflect effect of dietary ingredients on gut microbial community? 食事成分の腸内細菌叢への影響を代謝産物から読み解けるか? 日本細菌学雑誌(Web) 1882-4110 72 1 null null 20170000 6339428/misc/47952210 HATAYAMA Sho SHIMOHATA Takaaki AMANO Sachie KIDO Junko KANDA Yuna TENTAKU Aya FUKUSHIMA Shiho UEBANSO Takeshi MAWATARI Kazuaki TAKAHASHI Akira Regulation of Campylobacter jejuni invasion by Tight Jnuctions in polarized intestinal epithelial cells 極性化腸管上皮細胞でのタイトジャンクションによるCampylobacter jejuniの侵入制御について 日本細菌学雑誌(Web) 1882-4110 72 1 null null 20170000 6339428/misc/47952205 MAWATARI Kazuaki MAETANI-YASUI Miki MAETANI-YASUI Miki HONJO Airi NISHISAKA Risa EDAGAWA Miyuki IWAMOTO Natsumi WATANABE Hitomi SHIMOHATA Takaaki UEBANSO Takashi TAKAHASHI Akira Screening of genes related with ultraviolet A-sensitivity by transposon mutagenesis in Vibrio parahaemolyticus 腸炎ビブリオにおけるトランスポゾン変異誘発による紫外線A感受性と関連する遺伝子のスクリーニング 日本細菌学雑誌(Web) 1882-4110 72 1 null null 20170000 6339428/misc/19337647 Mutsumi Nakahashi 中橋 睦美 Akira Takahashi ?橋 章 紫外線LEDの医療への応用 Pediatrics of Japan 小児科 0037-4121 57 8 1011 1016 20160700 6339428/misc/19337648 Mutsumi Nakahashi 中橋 睦美 Akira Takahashi ?橋 章 紫外線LEDを用いた殺菌システムと応用 (特集 先進的環境プロセス技術の展開) 化学工業社 ケミカルエンジニヤリング = Chemical engineering 0387-1037 61 6 428 432 20160600 6339428/misc/47952144 下畑隆明 福島志帆 佐藤優里 扶川留音 木戸純子 神田結奈 天宅あや 畑山翔 中橋睦美 上番増喬 原田永勝 馬渡一諭 高橋章 上皮細胞に侵入したCampylobacter jejuniのエネルギー獲得機構について 日本食品微生物学会学術総会講演要旨集 37th null null 20160000 6339428/misc/47952139 常冨愛香里 下畑隆明 永田早紀恵 天野幸恵 中橋睦美 原田優美 上番増喬 馬渡一諭 宮脇克行 榎本崇宏 芥川正武 木内陽介 高橋章 Campylobacter jejuni食中毒予防に対するUVA-LED照射殺菌の有効性について 日本食品微生物学会学術総会講演要旨集 37th null null 20160000 6339428/misc/47952134 中橋睦美 常冨愛香里 上番増喬 下畑隆明 馬渡一諭 芥川正武 木内陽介 高橋章 紫外線LEDと次亜塩素酸ナトリウムの併用による食品殺菌装置の開発 日本食品微生物学会学術総会講演要旨集 37th null null 20160000 6339428/misc/47952130 高橋章 下畑隆明 馬渡一諭 上番増喬 常冨愛香里 中橋睦美 芥川正武 木内陽介 飲料水の色が近紫外線殺菌に与える影響 日本食品微生物学会学術総会講演要旨集 37th null null 20160000 6339428/misc/47952251 UEBANSO Takashi AI Ohnishi SHIMOHATA Takaaki NAKAHASHI Mutsumi MAWATARI Kazuaki TAKAHASHI Akira Acceptable daily intake levels of sucralose consumption reduces fecal Clostridium cluster 4 in mouse 一日摂取許容量のスクラロース摂取はマウスにおいて糞便Clostridiumクラスタを低減する 日本栄養・食糧学会大会講演要旨集 12th Asian Congress of Nutrition null null 20150000 6339428/misc/47952247 YOSHIMOTO Ayumi UEBANSO Takashi MAWATARI Kazuaki NAKAHASHI Mutsumi SHIMOHATA Takaaki TAKAHASHI Akira Low dose antibiotics treatment in the prenatal period affect newborns health in later life 出生前の低用量抗生物質処理は新生児の後の生活の健康に影響する 日本栄養・食糧学会大会講演要旨集 12th Asian Congress of Nutrition null null 20150000 6339428/misc/47952226 PHAN Ngoc Quang UEBANSO Takashi MAWATARI Kazuaki SHIMOHATA Takaaki AIHARA Mutsumi TAKAHASHI Akira DNA-binding protein HU coordinates pathogenicity in Vibrio parahaemolyticus. DNA結合蛋白質HUはVibrio parahaemolyticusの病原性を調整する. 四国医学雑誌 0037-3699 70 5/6 null null 20140000 6339428/misc/47952147 星山哲平 芥川正武 榎本崇宏 中橋睦美 林田麻里王 高橋章 馬渡一諭 池原敏孝 小中信典 木内陽介 UV及び可視光照射に対する腸炎ビブリオの遺伝子発現解析 電気関係学会四国支部連合大会講演論文集(CD-ROM) 2014 null null 20140000 6339428/misc/47952126 常冨愛香里 正村彰規 粟飯原睦美 馬渡一諭 下畑隆明 上番増喬 石崎仁愛 芥川正武 木内陽介 高橋章 近紫外線による水耕栽培用培養液の殺菌法の開発 日本栄養・食糧学会大会講演要旨集 68th null null 20140000 6339428/misc/19337649 Akira Takahashi ? Mutsumi Aihara n ? Kazuaki Mawatari H? ?? Masatake Akutagawa 近紫外線発光ダイオードを用いた殺菌システム 食品分野における非加熱殺菌技術 150 166 20131108 6339428/misc/19337650 Mutsumi Aihara 粟飯原 睦美 Akira Takahashi 橋 章 近紫外線発光ダイオードを用いた食肉表面殺菌法の開発 養牛の友 0385-0897 445 76 79 20130400 6339428/misc/19337651 常冨 愛香里 Mutsumi Aihara 粟飯原 睦美 Akira Takahashi 橋 章 Application and development to environmental purification of new UVA-LED 新UVA-LEDの環境浄化への応用と展開 (特集 紫外線利用による環境浄化の現状と課題) 日本空気清浄協会 空気清浄 : コンタミネーションコントロール 0023-5032 51 3 33 36 20130000 6339428/misc/47997990 GADELMOULA Mostafa LIAN Xin LIAN Xin HAMAMOTO Akiko MAWATARI Kazuaki YAMATO Masayuki AKUTAGAWA Masatake KINOUCHI Yohsuke TAKAHASHI Akira Novel Ultraviolet (A)-Light Emitting Diode for Air-Stream Disinfection UVA-LEDを用いた空気殺菌システムの開発 生体・生理工学シンポジウム論文集 25th null null 20100000 6339428/misc/47997987 MAWATARI Kazuaki TAKAHASHI Yukina MORIKAWA Nami SHIMOHATA Takaaki HAMAMOTO Akiko SU Zehong YAMATO Masayuki TAKAHASHI Akira VPA1604, a protein tyrosine kinase, modulate HMW K6-polysaccharide in Vibrio parahaemolyticus チロシンタンパクキナーゼVPA1604はVibrio parahaemolyticusの高分子量K6-polysaccharideの形成に関与する 日本細菌学雑誌 0021-4930 65 1 null null 20100000 6339428/misc/19337652 Mutsumi Aihara 粟飯原 睦美 Akira Takahashi 橋 章 食品製造分野におけるLED殺菌技術の可能性 (特集 洗浄・殺菌技術の近況) 日本食品出版 ジャパンフ-ドサイエンス 0368-1122 48 6 20 26 20090600 6339428/misc/19337653 Yutaka Nakaya 中屋 豊 Kazuaki Mawatari 馬渡 一諭 Nagakatsu Harada 原田 永勝 Akira Takahashi 橋 章 侵襲時の栄養評価法 機能性食品と薬理栄養 4 4 215 218 20070900 6339428/misc/19337654 Yutaka Nakaya 中屋 豊 Nagakatsu Harada 原田 永勝 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Toshio Hosaka 保坂 利男 Control of hypertension in metabolic syndrome メタボリック症候群における高血圧の管理 Shikoku Acta Medica 四国医学雑誌 0037-3699 63 3,4 97 100 20070825 6339428/misc/19337655 Nakaya Yutaka Mawatari Kazuaki Takahashi Akira Harada Nagakatsu Hata Akiko Yasui Sonoko The phytoestrogen ginsensoside Re activates potassium channels of vascular smooth muscle cells through PI3K/Akt and nitric oxide pathways In vascular smooth muscle cells, large-conductance Ca<SUP>2+</SUP>-activated K<SUP>+</SUP> channels (K<SUB>Ca</SUB> channels) play a pivotal role in determining membrane potential, and thereby the vascular tone. Ginsenoside Re, a phytochemical from ginseng, is reported to activate this channel, but its precise mechanism is unsolved. Patch clamp studies showed that ginsenoside Re activates K<SUB>Ca</SUB> channels in the arterial smooth muscle cell line A10 in a dose-dependent manner. The channel-opening effect of ginsenoside Re was inhibited by 1 &micro;M L-NIO, an inhibitor of eNOS, but not by 3 &micro;M SMTC, an inhibitor of nNOS, indicating that ginsenoside Re activated K<SUB>Ca</SUB> channels through activation of eNOS. SH-6 (10 &micro;M), an Akt inhibitor, and wortmannin, a PI3-kinase inhibitor, completely blocked activation of K<SUB>Ca</SUB> channels by ginsenoside Re, indicating that it activates eNOS via a c-Src/PI3-kinase/Akt-dependent mechanism. In addition, the ginsenoside Re-induced activation of eNOS and K<SUB>Ca</SUB> channel was blocked by 10 &micro;M ICI 182, 780, an inhibitor of membrane estrogen receptor-&alpha;, suggesting that eNOS activation occurs via a non-genomic pathway of this receptor. In conclusion, ginsenoside Re releases NO via a membrane sex steroid receptors, resulting in K<SUB>Ca</SUB> channel activation in vascular smooth muscle cells, promoting vasodilation and preventing severe arterial contraction. J. Med. Invest. 54: 381-384, August, 2007 Faculty of Medicine Tokushima University 国立大学法人 徳島大学医学部 The Journal of Medical Investigation 1343-1420 54 3 381 384 20070000 10.2152/jmi.54.381 17878692 6339428/misc/19337656 Seiichiro Kitamura 北村 清一郎 Chihiro Shinohara 篠原 千尋 Akira Takahashi 橋 章 見える!わかる!臨床に役立つ解剖写真集?7 歯界展望 108 1 72 78 20060000 6339428/presentations/16422579 Reiko Nakao 中尾 玲子 Katsuyuki Miyawaki 宮脇 克行 Yoshihiro Deguchi 出口 祥啓 Akira Takahashi 橋 章 Takeshi Nikawa 二川 健 宇宙栄養・食糧関連技術の開発とGatewayへの期待 国際宇宙探査ワークショップ(その2) 20190301 6339428/presentations/16422748 Yohsuke Kinouchi 木内 陽介 Masanobu Haraguchi 原口 雅宣 Akira Takahashi 橋 章 Toshiya Okahisa 岡久 稔也 徳島大学ライフオプティクス研究プロジェクトの進展 LED総合フォーラム2019in徳島 20190223 6339428/presentations/16422747 Masanobu Haraguchi 原口 雅宣 Takahiro Kitada 北田 貴弘 Masao Nagase 永瀬 雅夫 Takeshi Yasui 安井 武史 Yohsuke Kinouchi 木内 陽介 Katsuyuki Miyawaki 宮脇 克行 Akira Takahashi 橋 章 Toshiya Okahisa 岡久 稔也 LEDライフイノベーション総合プラットフォーム推進事業の取り組み LED総合フォーラム2019in徳島 20190223 6339428/presentations/16422751 Mai Tsujiguchi 辻口 舞 Takaaki Shimohata 下畑 隆明 Shiho Fukushima 福島 志帆 Sho Hatayama 畑山 翔 Junko Kido 木戸 純子 Kai Ishida 石田 快 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Microarrayの解析から導くCampylobacter jejuniの侵入による宿主腸管上皮細胞での脂質代謝への影響 第11回 日本カンピロバクター研究会総会 20181201 6339428/presentations/16422750 Kai Ishida 石田 快 Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Junko Kido 木戸 純子 Yuna Kanda 神田 結奈 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuniの上皮細胞内生存とコレステロールの関連 第11回 日本カンピロバクター研究会総会 20181201 6339428/presentations/16422749 Sho Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 Junko Kido 木戸 純子 Yuna Kanda 神田 結奈 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Mutsumi Nakahashi 中橋 睦美 Akiko Nakamoto 中本 晶子 Emi Shuto 首藤 恵泉 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 UVA-LED照射による調理器具汚染Campylobacter jejuniの殺菌効果について 第11回 日本カンピロバクター研究会総会 20181201 6339428/presentations/16422580 Hiroki Mori 森 大樹 Yuji Morine 森根 裕二 横田 典子 Hiroki Ishibashi 石橋 広樹 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Mitsuo Shimada 島田 光生 膵・胆管合流異常における発がんメカニズムに関する検討 第80回日本臨床外科学会総会 20181122 6339428/presentations/16422752 Takeshi Nikawa 二川 健 Akira Takahashi ?橋 章 Katsuyuki Miyawaki 宮脇 克行 Yoshihiro Deguchi 出口 祥啓 無重力や寝たきりによる筋萎縮の分子メカニズムとその栄養学的アプローチ ナノオプティクス研究グループ 第25回研究討論会 20181120 6339428/presentations/16422582 Yuna Kanda 神田 結奈 Ryo Higashiyama 東山 諒 Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Junko Kido 木戸 純子 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Kai Ishida 石田 快 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 柏本 孝茂 Akira Takahashi 橋 章 Vibrio Vulnificusが産生するOMVの機能解析 第71回 日本細菌学会中国・四国支部総会 20181006 6339428/presentations/16422581 Shiho Fukushima 福島 志帆 Takaaki Shimohata 下畑 隆明 Junko Kido 木戸 純子 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni感染で誘導されるオートファジーは宿主細胞への菌の侵入および細胞内生存を促進する 第71回 日本細菌学会中国・四国支部総会 20181006 6339428/presentations/16422584 Sho Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 Junko Kido 木戸 純子 Yuna Kanda 神田 結奈 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Mutsumi Nakahashi 中橋 睦美 Akiko Nakamoto 中本 晶子 Emi Shuto 首藤 恵泉 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni汚染調理器具に対するUVA-LED照射殺菌の有効性について 第39回 日本食品微生物学会学術総会 20180928 6339428/presentations/16422583 Aya Tentaku 天宅 あや Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Anh Quoc Nguyen NGUYEN Junko Kido QUOC ANH Shiho Fukushima 木戸 純子 Takashi Uebansou 福島 志帆 Kazuaki Mawatari 上番増 喬 Akira Takahashi 馬渡 一諭 橋 章 Campylobacter jejuni感染によって誘導される小胞体ストレス応答は菌の細胞侵入を抑制する 第39回 日本食品微生物学会学術総会 20180928 6339428/presentations/16422586 Yuna Kanda 神田 結奈 Ryo Higashiyama 東山 諒 Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Junko Kido 木戸 純子 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Kai Ishida 石田 快 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 柏本 孝茂 Akira Takahashi 橋 章 Vibrio Vulnificusが産生するOMVの機能解析 第39回 日本食品微生物学会学術総会 20180927 6339428/presentations/16422585 Yu Saitou 齋藤 裕 Hiroki Mori 森 大樹 Yuji Morine 森根 裕二 Keigo Yada 矢田 圭吾 Hiroki Ishibashi 石橋 広樹 馬渡 一論 Akira Takahashi 橋 章 Mitsuo Shimada 島田 光生 メタボローム解析を用いた膵・胆管合流異常の胆汁中発癌物質の同定 千葉 第54回日本胆道学会学術集会 20180927 6339428/presentations/16422587 Shiho Fukushima 福島 志帆 Takaaki Shimohata 下畑 隆明 Junko Kido 木戸 純子 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 オートファジーはCampylobacter jejuniの宿主細胞への侵入過程を促進する 第91回 日本生化学会大会 20180926 6339428/presentations/16422588 Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Junko Kido 木戸 純子 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuniは宿主腸管上皮細胞の細胞側面から効率的に侵入する 第91回 日本生化学会大会 20180925 6339428/presentations/16422521 Yukiko Tomioka Hidehiro Umehara Shinya Watanabe Masahito Nakataki Masuda Rumiko Kazuaki Mawatari Takeshi Nikawa Akira Takahashi Shusuke Numata Tetsuro Ohmori Altered plasma metabolites related to one-carbon metabolism in schizophrenia. WFSBP Asia Pacific Regional Congress of Biological Psychiatry 20180909 6339428/presentations/16422753 Aya Tentaku 天宅 あや Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Anh Quoc Nguyen NGUYEN Junko Kido QUOC ANH Shiho Fukushima 木戸 純子 Kai Ishida 福島 志帆 Takashi Uebansou 石田 快 Kazuaki Mawatari 上番増 喬 Akira Takahashi 馬渡 一諭 橋 章 宿主細胞における小胞体ストレス応答はCampylobacter jejuniの細胞内侵入を抑制する 第257回 徳島医学会学術集会 20180805 6339428/presentations/16422589 Ulfa Maria Takaaki Shimohata Shiho Fukushima Momoyo Azuma Takashi Uebansou Kazuaki Mawatari Akira Takahashi Application of UVA-LED on Extended-Spectrumβ-Lactamase(ESBL) Producing Escherichia coli from Clinical Isolates 第257回 徳島医学会学術集会 20180805 6339428/presentations/16422522 Toshiya Okahisa Masahiro Sogabe Mayu Uyama Tadahiko Nakagawa Tetsu Tomonari Tatsuya Taniguchi Koichi Okamoto Tetsuji Takayama Takaaki Shimohata Takashi Uebansou Kazuaki Mawatari Akira Takahashi Takahiro Emoto Masatake Akutagawa M Yamada M Fukuhara Development Of A Multi-Ring Type Roller Pump Unit Equipped To A Compact And Convenient Ascites Purification Machine For Cell-Free And Concentrated Ascites Reinfusion Therapy (CART). ASAIO 64th Annual Conference 20180616 6339428/presentations/16422524 Shiho Fukushima Yuri Sato Takaaki Shimohata Junko Kido Yuna Kanda Aya Tentaku Takashi Uebansou Kazuaki Mawatari Akira Takahashi Campylobacter jejuni Utilized Autophagy for the Bacterial Survival in the Host Epithelial Cells 118th General Meeting of the American Society for Microbiology 20180608 6339428/presentations/16422523 Aya Tentaku Takaaki Shimohata Sho Hatayama Anh Quoc Nguyen Junko Kido Shiho Fukushima Takashi Uebansou Kazuaki Mawatari Akira Takahashi Induction of the unfolded protein response (UPR) decreased Campylobacter jejunin invasion 118th General Meeting of the American Society for Microbiology 20180608 6339428/presentations/16422591 Shiho Fukushima 福島 志帆 Takaaki Shimohata 下畑 隆明 Junko Kido 木戸 純子 Aya Tentaku 天宅 あや Kai Ishida 石田 快 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni感染で誘導されるオートファジーは宿主細胞への侵入および細胞内生存を促進する 第92回 日本感染症学会学術講演会 20180531 6339428/presentations/16422590 Kai Ishida 石田 快 Takaaki Shimohata 下畑 隆明 Junko Kido 木戸 純子 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuniの宿主腸管上皮細胞での生存に対するコレステロールの影響 第92回 日本感染症学会学術講演会 20180531 6339428/presentations/16422592 Hiroki Mori 森 大樹 Yuji Morine 森根 裕二 Keigo Yada 矢田 圭吾 Hiroki Ishibashi 石橋 広樹 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Mitsuo Shimada 島田 光生 メタボローム解析を用いた膵・胆管合流異常における胆汁発癌物質の検討 第55回日本小児外科学会学術集会 20180530 6339428/presentations/16422593 Aya Tentaku 天宅 あや Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Anh Quoc Nguyen NGUYEN Junko Kido QUOC ANH Shiho Fukushima 木戸 純子 Kai Ishida 福島 志帆 Takashi Uebansou 石田 快 Kazuaki Mawatari 上番増 喬 Akira Takahashi 馬渡 一諭 橋 章 宿主細胞内での小胞体ストレス応答(UPR)はCampylobacter jejuni感染に対する防御機構として働く 第72回 日本栄養・食糧学会大会 20180513 6339428/presentations/16422594 Hiroki Mori 森 大樹 Yuji Morine 森根 裕二 Keigo Yada 矢田 圭吾 Hiroki Ishibashi 石橋 広樹 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Mitsuo Shimada 島田 光生 メタボローム解析を用いた膵・胆管合流異常の胆汁中発癌物質の同定 第118回日本外科学会定期学術集会 20180405 6339428/presentations/16422595 Quoc Nguyen anh Takaaki Shimohata sho Hatayama aya Tentaku Junko kido Takashi Uebansou Kazuaki Mawatari Akira Takahashi Metabolic analysis of epithelial cellular metabolism during Vibrio parahaemolyticus infection 第91回 日本細菌学会総会 20180328 6339428/presentations/16422597 shiho Fukushima 福島 志帆 Takaaki Shimohata 下畑 隆明 sho Hatayama 畑山 翔 Junko KIdo 木戸 純子 aya Tentaku 天宅 あや yuna kanda 神田 結奈 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuniは菌の宿主上皮細胞への侵入および生存過程にオートファジーを利用する 第91回 日本細菌学会総会 20180327 6339428/presentations/16422596 aya tentaku 天宅 あや Takaaki Shimohata 下畑 隆明 sho Hatayama 畑山 翔 Quoc Nguyen Anh NGUYEN Junko kido QUOC ANH shiho fukushima 木戸 純子 Takashi Uebansou 福島 志帆 Kazuaki Mawatari 上番増 喬 Akira Takahashi 馬渡 一諭 橋 章 Campylobacter jejuni感染における小胞体ストレス応答は菌の侵入を抑制する 第91回 日本細菌学会総会 20180327 6339428/presentations/16422525 Junko Kido Takaaki Shimohata sachie amano sho hatayama yuri sato yuna kanda aya tentaku shiho Fukushima Mutsumi Nakahashi Takashi Uebansou Kazuaki Mawatari Akira Takahashi CFTR reduced microtubule-mediated Campylobacter jejuni invasion 52th US-Japan Cooperative Medical Sciences Program Conference on cholera and Other Bacterial Enteric Infections 20180222 6339428/presentations/16422754 Masanobu Haraguchi 原口 雅宣 Takahiro Kitada 北田 貴弘 Masao Nagase 永瀬 雅夫 Takeshi Yasui 安井 武史 Yohsuke Kinouchi 木内 陽介 Katsuyuki Miyawaki 宮脇 克行 Akira Takahashi 橋 章 Toshiya Okahisa 岡久 稔也 LEDライフイノベーション総合プラットフォーム推進事業におけるテラヘルツLED応用基盤技術に関する取り組み LED総合フォーラム2018in徳島 20180212 6339428/presentations/16422598 Shiho Fukushima 福島 志帆 Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Junko Kido 木戸 純子 Aya Tentaku 天宅 あや Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni感染で誘導されるオートファジーは，宿主上皮細胞における菌の侵入および生存を促進する 第256回 徳島医学会学術集会 20180211 6339428/presentations/16422599 junko kido 木戸 純子 Takaaki Shimohata 下畑 隆明 sachie amano 天野 幸恵 sho hatayama 畑山 翔 aya Tentaku 天宅 あや shiho fukushima 福島 志帆 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 CFTR発現はCampylobacter jejuniの微小管を介した輸送を抑制する ConBio2017 2017年度 生命科学系学会合同年次大会 20171209 6339428/presentations/16422600 aya Tentaku 天宅 あや Takaaki Shimohata 下畑 隆明 sho Hatayama 畑山 翔 Quoc Nguyen Anh NGUYEN junko kido QUOC ANH shiho fukushima 木戸 純子 Takashi Uebansou 福島 志帆 Kazuaki Mawatari 上番増 喬 Akira Takahashi 馬渡 一諭 橋 章 Campylobacter jejuni感染で誘導される小胞体ストレス応答は細胞内への菌の侵入を抑制する ConBio2017 2017年度 生命科学系学会合同年次大会 20171208 6339428/presentations/16422601 shiho Fukushima 福島 志帆 Takaaki Shimohata 下畑 隆明 sho Hatayama 畑山 翔 junko kido 木戸 純子 aya Tentaku 天宅 あや Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni感染で誘導されるオートファジーは，宿主上皮細胞における菌の侵入および生存を促進する ConBio2017 2017年度 生命科学系学会合同年次大会 20171206 6339428/presentations/16422756 Shiho Fukushima 福島 志帆 Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Junko Kido 木戸 純子 Aya Tentaku 天宅 あや Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuniが誘導するオートファジーが菌の侵入および生存に与える影響について 第10回 日本カンピロバクター研究会総会 20171201 6339428/presentations/16422755 Akari Tsunedomi 常冨 愛香里 Sho Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 Junko Kido 木戸 純子 Sachie Amano 天野 幸恵 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 Campylobacter jejuni汚染対策に向けたUVA-LEDの有用性について 第10回 日本カンピロバクター研究会総会 20171201 6339428/presentations/16422604 Aya Tentaku 天宅 あや Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Anh Quoc Nguyen NGUYEN Junko Kido QUOC ANH Shiho Fukushima 木戸 純子 Takashi Uebansou 福島 志帆 Kazuaki Mawatari 上番増 喬 Akira Takahashi 馬渡 一諭 橋 章 Cpylobacter jejuni感染によって誘導される小胞体ストレス応答が菌に与える影響 第70回 日本細菌学会中国・四国支部総会 20171014 6339428/presentations/16422603 Anh Quoc Nguyen Takaaki Shimohata Sho Hatayama Aya Tentaku Junko Kido Takashi Uebansou Kazuaki Mawatari Akira Takahashi Metabolic analysis of epithelial cellular metabolism during Vibrio parahaemolyticus infection 第70回 日本細菌学会中国・四国支部総会 20171014 6339428/presentations/16422602 Takaaki Shimohata 下畑 隆明 Junko Kido 木戸 純子 Sho Hatayama 畑山 翔 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 代謝産物の解析から紐解くCampylobacter jejuniの宿主細胞内生存戦略 第70回 日本細菌学会中国・四国支部総会 20171014 6339428/presentations/16422606 Shiho Fukushima 福島 志帆 Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Junko Kido 木戸 純子 Aya Tentaku 天宅 あや Yuna Kanda 神田 結奈 Ryoka Narutaki 鳴滝 涼香 Kai Ishida 石田 快 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni感染で誘導されるオートファジーは菌のクリアランス機構として働くのか 第38回 日本食品微生物学会学術総会 20171005 6339428/presentations/16422605 Sho Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 Junko Kido 木戸 純子 Yuna Kanda 神田 結奈 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 極性化腸管上皮細胞におけるタイトジャンクション形成はCampylobacter jejuniの細胞内侵入機構に影響する 第38回 日本食品微生物学会学術総会 20171005 6339428/presentations/16422607 Hirotaka Motoyama 本山 裕隆 Masatake Akutagawa 芥川 正武 Takahiro Emoto 榎本 崇宏 Emiko Yasuno 安野 恵実子 Takaaki Shimohata 下畑 隆明 Akira Takahashi ?橋 章 Shinsuke Konaka 小中 信典 Yohsuke Kinouchi 木内 陽介 Study on electrode structure for measurement of electrical impedance of the human intestine using the finite element method 有限要素法を用いたヒト腸管インピーダンス測定用電極の検討 2017 Shikoku-Section Joint Convention Record of the Institutes of Electrical and Related Engineers (EHIME) 平成29年度電気関係学会四国支部連合大会講演論文集(2017 愛媛大学) 20170923 6339428/presentations/16422608 Junko Kido 木戸 純子 Takaaki Shimohata 下畑 隆明 Sachie Amano 天野 幸恵 Syo Hatayama 畑山 翔 Yuri Sato 佐藤 優里 Yuna Kanda 神田 結奈 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 CFTR reduced microtubule-mediated Campylobacter jejuni invasion 日米医学協力研究会コレラ・細菌性腸管感染症専門部会 20170808 6339428/presentations/16422757 Kazuaki Mawatari 馬渡 一諭 Hitomi Watanabe 渡邊 瞳 Risa Nishisaka 西坂 理沙 Mutsumi Nakahashi 中橋 睦美 Akari Tsunedomi 常冨 愛香里 Mizuki Kojima 児島 瑞基 Natsuki Nogami 野上 夏希 Misuzu Makino 牧野 美鈴 Rumiko Masuda 増田 瑠見子 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 Bacterial contamination in dialysis fluid and bactericidal effects of near UV-light emitting diodes 透析液の細菌汚染調査と近紫外発光ダイオードによる殺菌効果の評価 Institute of Electronics, Information and Communication Engineers 電子情報通信学会 IEICE Technical Report (MBE) 電子情報通信学会技術研究報告(MEとバイオサイバネティックス) 20170729 6339428/presentations/16422610 Sho Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 Sachie Amano 天野 幸恵 Junko Kido 木戸 純子 神田 結奈 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 宿主腸管上皮細胞のTight Junction形成が食中毒原因菌Campylobacter jejuniの侵入機構に及ぼす影響について 日本栄養食糧学会 20170520 6339428/presentations/16422609 Takashi Uebansou 上番増 喬 Ai Ohnishi 大西 愛 Reiko Kitayama 北山 礼子 Ayumi Yoshimoto 吉本 亜由美 Mutsumi Nakahashi 中橋 睦美 Takaaki Shimohata 下畑 隆明 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 許容上限量の甘味料の摂取が腸内細菌叢と宿主へ及ぼす影響の解析 日本栄養食糧学会 20170520 6339428/presentations/16422611 Aya Tentaku 天宅 あや Takaaki Shimohata 下畑 隆明 Junko Kido 木戸 純子 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni感染における小胞体ストレスの誘導 第91回 日本感染症学会総会・学術講演会 20170408 6339428/presentations/16422613 Takaaki Shimohata 下畑 隆明 Junko Kido 木戸 純子 Yuri Sato 佐藤 優里 Sho Hatayama 畑山 翔 Yuna Kanda 神田 結奈 Shiho Fukushima 福島 志帆 Aya Tentaku 天宅 あや Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni 感染はHeLa細胞におけるアミノ酸取り込みを活性化する 日本細菌学会総会 20170320 6339428/presentations/16422612 Sho Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 Sachie Amano 天野 幸恵 Junko Kido 木戸 純子 Yuna Kanda 神田 結奈 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 極性化腸管上皮細胞でのタイトジャンクションによるCampylobaster Jejuniの侵入制御について 日本細菌学会総会 20170320 6339428/presentations/16422615 Takashi Uebansou 上番増 喬 吉本 亜由美 Takaaki Shimohata 下畑 隆明 Mutsumi Nakahashi 中橋 睦美 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 食事成分の腸内細菌叢への影響を代謝産物から読み解けるか? 日本細菌学会総会 20170319 6339428/presentations/16422614 Kazuaki Mawatari 馬渡 一諭 Miki Yasui 安井 実希 Airi Honjoh 本庄 アイリ Risa Nishisaka 西坂 理沙 枝川 美幸 岩本 夏美 Hitomi Watanabe 渡邊 瞳 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Akira Takahashi 橋 章 Screening of genes related with ultraviolet A-sensitivity by transposon mutagenesis in Vibrio parahaemolyticus 90th Annual Meeting of Japanese Society of bacteriology 20170319 6339428/presentations/16422526 Syo Hatayama Takaaki Shimohata Takashi Uebansou Kazuaki Mawatari Akira Takahashi The relation of cellular Tight Junctions formation and Campylobacter jejuni invasion in intestinal epithelial cells 51th US-Japan Cooperative Medical Sciences Program Conference on cholera and Other Bacterial Enteric Infections 20170209 6339428/presentations/16422758 Masanobu Haraguchi 原口 雅宣 Yohsuke Kinouchi 木内 陽介 Takahiro Kitada 北田 貴弘 Masao Nagase 永瀬 雅夫 Takeshi Yasui 安井 武史 Katsuyuki Miyawaki 宮脇 克行 Akira Takahashi 橋 章 Toshiya Okahisa 岡久 稔也 LEDライフイノベーション総合プラットフォーム推進事業におけるテラヘルツLED応用基盤技術に関する取り組み LED総合フォーラム実行委員会 LED総合フォーラム2016in徳島 論文集 20161217 6339428/presentations/16422761 Aya Tentaku 天宅 あや Takaaki Shimohata 下畑 隆明 Syo Hatayama 畑山 翔 Junko Kido 木戸 純子 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Nagakatsu Harada 原田 永勝 Akira Takahashi 橋 章 Campylobacter jejuni感染で誘導される小胞体ストレスについて 第9回 日本カンピロバクター研究会総会 20161126 6339428/presentations/16422760 Junko Kido 木戸 純子 Takaaki Shimohata 下畑 隆明 Yuri Sato 佐藤 優里 Syo Hatayama 畑山 翔 Yuna Kanda 神田 結奈 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 宿主アミノ酸輸送の変化と宿主細胞内Campylobacter jejuni生存について 第9回 日本カンピロバクター研究会総会 20161126 6339428/presentations/16422759 Syo Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 Sachie Amano 天野 幸恵 Junko Kido 木戸 純子 Yuna Kanda 神田 結奈 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuniの宿主腸管上皮細胞への侵入とTight Junctions形成の関連について 第9回 日本カンピロバクター研究会総会 20161126 6339428/presentations/16422616 Mayu Uyama 宇山 真由 Masahiro Sogabe 曽我部 正弘 平田 光里 Tadahiko Nakagawa 中川 忠彦 Akira Fukuya 福家 慧 Satoshi Teramae 寺前 智史 Daisaku Fujimoto 藤本 大策 Hironori Tanaka 田中 宏典 Yasuhiro Mitsui 三井 康裕 Shinji Kitamura 北村 晋志 Koichi Okamoto 岡本 耕一 Tetsuji Takayama 高山 哲治 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi ?橋 章 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Rumi Katashima 片島 るみ Toshiya Okahisa 岡久 稔也 模擬腹水による胸腹水濾過濃縮再静注法の教育体制の構築 第37回日本アフェレシス学会学術大会 20161126 6339428/presentations/16422617 Ryuto Tamai 玉井 瑠人 Hiroto Kasai 笠井 嘉人 Daiki Tanaka 田中 大基 Naoya Tani 谷 直也 平田 光里 Tadahiko Nakagawa 中川 忠彦 Tetsu Tomonari 友成 哲 Tatsuya Taniguchi 谷口 達哉 Koichi Okamoto 岡本 耕一 Hiroshi Miyamoto 宮本 弘志 Tetsuji Takayama 高山 哲治 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi ?橋 章 Mayu Uyama 宇山 真由 Masahiro Sogabe 曽我部 正弘 Toshiya Okahisa 岡久 稔也 胸腹水濾過濃縮再静注法のクロスフロー方式の腹水濾過時の物理的刺激が腹水に及ぼす影響に関する実験的検討. 第54回日本人工臓器学会大会 20161124 6339428/presentations/16422620 Junko Kido 木戸 純子 Takaaki Shimohata 下畑 隆明 Sachie Amano 天野 幸恵 Syo Hatayama 畑山 翔 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 CFTR発現によるCampylobacter jejuniの侵入抑制機序の解明 第69回 日本細菌学会中国・四国支部総会 20161015 6339428/presentations/16422619 Takaaki Shimohata 下畑 隆明 Shiho Fukushima 福島 志帆 Yuri Sato 佐藤 優里 Rune Fukawa 扶川 留音 Junko Kido 木戸 純子 Yuna Kanda 神田 結奈 Aya Tentaku 天宅 あや Syo Hatayama 畑山 翔 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuniの宿主細胞内生存戦力に関する検討 第69回 日本細菌学会中国・四国支部総会 20161015 6339428/presentations/16422618 Hitomi Watanabe 渡邊 瞳 Kazuaki Mawatari 馬渡 一諭 Risa Nishisaka 西坂 理沙 Mutsumi Nakahashi 中橋 睦美 Akari Tsunedomi 常冨 愛香里 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 透析液の細菌汚染調査と近視外LEDによる殺菌効果の評価 第69回 日本細菌学会中国・四国支部総会 20161015 6339428/presentations/16422623 Akari Tsunedomi 常冨 愛香里 Takaaki Shimohata 下畑 隆明 Sakie Nagata 永田 早紀恵 Sachie Amano 天野 幸恵 Mutsumi Nakahashi 中橋 睦美 Yumi Harada 原田 優美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Katsuyuki Miyawaki 宮脇 克行 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 Campylobacter jejuni食中毒予防に対するUVA-LED照射殺菌の有用性について 第37回 日本食品微生物学会学術総会 20160916 6339428/presentations/16422622 Akira Takahashi ? Takaaki Shimohata n Kazuaki Mawatari H? Takashi Uebansou ?? Akari Tsunedomi ?z? Mutsumi Nakahashi Masatake Akutagawa Yohsuke Kinouchi 飲料水の色が近紫外線殺菌にあたえる影響 第37回 日本食品微生物学会学術総会 20160916 6339428/presentations/16422621 Mutsumi Nakahashi 中橋 睦美 Akari Tsunedomi 常冨 愛香里 Takashi Uebansou 上番増 喬 Takaaki Shimohata 下畑 隆明 Kazuaki Mawatari 馬渡 一諭 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 紫外線LEDと次亜塩素酸ナトリウムの併用による食品殺菌装置の開発 第37回 日本食品微生物学会学術総会 20160916 6339428/presentations/16422624 Takaaki Shimohata 下畑 隆明 Shiho Fukushima 福島 志帆 Yuri Sato 佐藤 優里 Rune Fukawa 扶川 留音 Junko Kido 木戸 純子 Kanda Yuna 神田 結奈 Aya Tentaku 天宅 あや Syo Hatayama 畑山 翔 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Nagakatsu Harada 原田 永勝 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 上皮細胞に侵入したCampylobacter jejuniのエネルギー獲得機構について 第37回 日本食品微生物学会学術総会 20160915 6339428/presentations/16422625 Sho Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 Sachie Negoro 根来 幸恵 Junko Kido 木戸 純子 Hitomi Iba 射場 仁美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Disruption of tight junctions promote lipid raft-mediated Campylobacter jejuni invasion at lateral part of the intestinal cells 腸管上皮細胞におけるtight junctionsの破綻は細胞側面からの脂質ラフトを介したCampylobacter jejuniの侵入を促進する 日米医学協力研究会コレラ・細菌性腸管感染症専門部会 20160805 6339428/presentations/16422628 Ayumi Yoshimoto 吉本 亜由美 Takashi Uebansou 上番増 喬 Takaaki Shimohata 下畑 隆明 Mutsumi Nakahashi 中橋 睦美 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 妊娠期の母親の低用量の抗菌薬摂取が子供の健康に及ぼす影響の解析 第253回徳島医学会学術集会 20160724 6339428/presentations/16422627 Junko Kido 木戸 純子 Takaaki Shimohata 下畑 隆明 Sachie Negoro 根来 幸恵 Sho Hatayama 畑山 翔 Aya Tentaku 天宅 あや Shiho Fukushima 福島 志帆 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 CFTR発現によりCampylobacter jejuniの微小管依存性侵入機構が抑制される 第253回徳島医学会学術集会 20160724 6339428/presentations/16422626 Risa Nishisaka 西坂 理沙 Hitomi Watanabe 渡邊 瞳 Kazuaki Mawatari 馬渡 一諭 Mutsumi Nakahashi 中橋 睦美 Akari Tsunedomi 常冨 愛香里 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 県内医療施設の透析液細菌汚染調査と近紫外LEDによる殺菌効果の評価 第253回徳島医学会学術集会 20160724 6339428/presentations/16422630 Akari Tsunedomi 常冨 愛香里 Takaaki Shimohata 下畑 隆明 Marin Gotoh 後藤 茉凜 Sachie Amano 天野 幸恵 Mutsumi Nakahashi 中橋 睦美 原田 優美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Katsuyuki Miyawaki 宮脇 克行 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 UVA-LED殺菌システムによるCampylobacter jejuni食中毒の予防 第70回日本栄養・食糧学会大会 20160514 6339428/presentations/16422629 Sho Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 Michiko Yoshikane 吉兼 道子 Sachie Amano 天野 幸恵 Yuri Sato 佐藤 優里 Junko Kido 木戸 純子 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 食中毒原因菌Campylobacter jejuniは腸管上皮細胞側面の露出により宿主内へ効率的に侵入する 第70回日本栄養・食糧学会大会 20160514 6339428/presentations/16422631 Mayu Uyama 宇山 真由 Masahiro Sogabe 曽我部 正弘 Hirata Misato 平田 光里 Tadahiko Nakagawa 中川 忠彦 Takaoka Yoshifumi 高岡 慶史 Tatsuya Taniguchi 谷口 達哉 Tetsuji Takayama 高山 哲治 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Toshiya Okahisa 岡久 稔也 胸腹水濾過濃縮再静注法の手技習得のための輸液製剤を用いた模擬腹水の作成 第43回日本血液浄化技術学会学術大会 20160501 6339428/presentations/16422633 Risa Nishisaka 西坂 理沙 Kazuaki Mawatari 馬渡 一諭 Tomoko Yamashita 山下 智子 Junko Kido 木戸 純子 Akari Tsunedomi 常冨 愛香里 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Akira Takahashi 橋 章 透析液の汚染細菌調査と近紫外発光ダイオードを用いた殺菌効果の評価 第90回日本感染症学会総会・学術講演会 20160416 6339428/presentations/16422632 Akari Tsunedomi 常冨 愛香里 Junko Kido 木戸 純子 Takashi Uebansou 上番増 喬 Takaaki Shimohata 下畑 隆明 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 UVA殺菌技術を用いた植物工場における病原微生物の抑制検討 第90回日本感染症学会総会・学術講演会 20160416 6339428/presentations/16422635 Takaaki Shimohata 下畑 隆明 Sato Yuri 佐藤 優里 Fukawa Rune 扶川 留音 Kido Junko 木戸 純子 Amano Sachie 天野 幸恵 Hatayama Sho 畑山 翔 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Metabolic analysis leveled the unique amino acid metabolism in Campylobacter jejuni infected cell 代謝解析で明らかとなったCampylobacter jejuni感染による宿主細胞での特徴的なアミノ酸変動について The 89th Annual Meeting of Japanese Society for Bacteriology 第89回日本細菌学会総会 20160324 6339428/presentations/16422634 Kido Junko 木戸 純子 Takaaki Shimohata 下畑 隆明 Negoro Sachie 根来 幸恵 Hatayama Sho 畑山 翔 Sato Yuri 佐藤 優里 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 The entry of Campylobacter jejuni was regulated by cell surface CFTR Campylobacter jejuniの細胞侵入は細胞膜表面CFTRにより調節される The 89th Annual Meeting of Japanese Society for Bacteriology 第89回日本細菌学会総会 20160324 6339428/presentations/16422638 Nishisaka Risa 西坂 理沙 Kazuaki Mawatari 馬渡 一諭 Tsunedomi Akari 常冨 愛香里 Watanabe Hitomi 渡邊 瞳 Uebanso Akiko 上番増 明子 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Kanemoto Yuki 金本 優杞 Murakami Akio 村上 明男 Akira Takahashi 橋 章 Ultraviolet-A (UVA) irradiation by light emitting diode (LED) inhibits growth of cyanobacteria The 89th Annual Meeting of Japanese Society for Bacteriology 第89回日本細菌学会総会 20160323 6339428/presentations/16422637 Kazuaki Mawatari 馬渡 一諭 Miyuki Edagawa 枝川 美幸 Natsumi Iwamoto 岩本 夏実 Miki Maetani 前谷 実希 Airi Honjo 本庄 アイリ Risa Nishisaka 西坂 理沙 Hitomi Watanabe 渡邊 瞳 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Akira Takahashi 橋 章 Identification of genes associated with ultraviolet-A sensitivity in Vibrio parahaemolyticus The 89th Annual Meeting of Japanese Society for Bacteriology 第89回日本細菌学会総会 20160323 6339428/presentations/16422636 Yoshimoto Ayumi 吉本 亜由美 Takashi Uebansou 上番増 喬 Mutsumi Nakahashi 中橋 睦美 Takaaki Shimohata 下畑 隆明 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Effect of low dose antibiotics administration during prenatal period on newborns postnatal health The 89th Annual Meeting of Japanese Society for Bacteriology 第89回日本細菌学会総会 20160323 6339428/presentations/16422763 Tsunedomi Akari 常冨 愛香里 Takaaki Shimohata 下畑 隆明 Gotoh Marin 後藤 茉凜 Amano Sachie 天野 幸恵 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Katsuyuki Miyawaki 宮脇 克行 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 Campylobacter jejuniに対するUVA-LEDの有効性 第8回日本カンピロバクター研究会総会 20151204 6339428/presentations/16422762 Sato Yuri 佐藤 優里 Takaaki Shimohata 下畑 隆明 Fukawa Rune 扶川 留音 Negoro Sachie 根来 幸恵 Hatayama Syo 畑山 翔 Kido Junko 木戸 純子 Takashi Uebansou 上番増 喬 Nagakatsu Harada 原田 永勝 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 メタボローム解析を用いた宿主細胞内のCampylobacter jejuniのエネルギー源獲得機構の検索 第8回日本カンピロバクター研究会総会 20151204 6339428/presentations/16422764 Kido Junko 木戸 純子 Takaaki Shimohata 下畑 隆明 Negoro Sachie 根来 幸恵 Hatayama Sho 畑山 翔 Sato Yuri 佐藤 優里 Fukawa Rune 扶川 留音 Yoshikane Michiko 吉兼 道子 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 CFTR発現によるCampylobacter jejuniの上皮細胞侵入抑制機構について 第8回日本カンピロバクター研究会総会 20151203 6339428/presentations/16422527 Asami Iwanaka Mamiko Soga Eiichi Kotake-Nara Akihito Nagao Rie Mukai Akira Takahashi Junji Terao Effect of Fucoxanthin and Neoxanthin on Blue Light-Emitting Diode-Irradiated Photosensitized Oxidation in Liposomal Membranes andMouse Fibroblast Cells The 6th International Conference on Food Factors: Bioconvergence for Food Function 20151122 6339428/presentations/16422640 Daiki Tanaka 田中 大基 Naoya Tani 谷 直也 Masanori Takehara 武原 正典 Hironori Tanaka 田中 宏典 Takahiro Tanaka 田中 貴大 Tetsu Tomonari 友成 哲 Tatsuya Taniguchi 谷口 達哉 Tadahiko Nakagawa 中川 忠彦 Tetsuji Takayama 高山 哲治 Masaki Kawano 河野 正樹 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi ?橋 章 Mayu Uyama 宇山 真由 Masahiro Sogabe 曽我部 正弘 Toshiya Okahisa 岡久 稔也 模擬腹水を用いた胸腹水濾過濃縮装置の評価・教育システムの構築 第53回日本人工臓器学会大会 20151120 6339428/presentations/16422639 谷 直也 Daiki Tanaka 田中 大基 Tatsunao Sueuchi 末内 辰尚 高岡 慶史 Tadahiko Nakagawa 中川 忠彦 Tetsuji Takayama 高山 哲治 Masaki Kawano 河野 正樹 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi ?橋 章 Mayu Uyama 宇山 真由 Masahiro Sogabe 曽我部 正弘 Toshiya Okahisa 岡久 稔也 イノベーション対話ツールを活用した腸蠕動音収集解析システムの開発 第53回日本人工臓器学会大会 20151120 6339428/presentations/16422641 Masaki Kawano 河野 正樹 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Mayu Uyama 宇山 真由 Masahiro Sogabe 曽我部 正弘 Tadahiko Nakagawa 中川 忠彦 Tetsuji Takayama 高山 哲治 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi ?橋 章 村島 徹 出口 喜宏 荒巻 広至 Toshiya Okahisa 岡久 稔也 腹水濾過濃縮法(CART)用装置の現状と今後 第36回日本アフェレシス学会学術総会 20151031 6339428/presentations/16422642 Katsuyuki Miyawaki 宮脇 克行 常冨 愛香里 後藤 茉凜 正村 彰規 Akira Takahashi 橋 章 Sumihare Noji 野地 澄晴 完全人工光型植物工場におけるイチゴ苗大量生産システムの開発 園芸学会 20150927 6339428/presentations/16422646 Syo Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 Michiko Yoshikane 吉兼 道子 Sachie Negoro 根来 幸恵 Yuri Sato 佐藤 優里 Junko Kido 木戸 純子 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuniの上皮細胞への侵入は細胞のラテラル面の露出により増加する 第251回 徳島医学会学術集会 20150802 6339428/presentations/16422645 Risa Nishisaka 西坂 理沙 Kazuaki Mawatari 馬渡 一諭 Akari Tsunedomi 常冨 愛香里 Marin Gotoh 後藤 茉凛 Akiko Uebanso 上番増 明子 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Yuki Kanemoto 金本 優杞 Akio Murakami 村上 明男 Akira Takahashi 橋 章 近紫外線(ultrviolet-A)照射によるシアノバクテリア増殖抑制効果の解明 第251回 徳島医学会学術集会 20150802 6339428/presentations/16422644 Akari Tsunedomi 常冨 愛香里 Akinori Masamura 正村 彰規 Mutsumi Nakahashi 中橋 睦美 Risa Nishisaka 西坂 理沙 Marin Gotoh 後藤 茉凛 Kazuaki Mawatari 馬渡 一諭 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Katsuyuki Miyawaki 宮脇 克行 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 植物工場の養液殺菌システムの開発 第251回 徳島医学会学術集会 20150802 6339428/presentations/16422643 Junko Kido 木戸 純子 Takaaki Shimohata 下畑 隆明 Sachie Negoro 根来 幸恵 Syo Hatayama 畑山 翔 Yuri Sato 佐藤 優里 Rune Fukawa 扶川 留音 Michiko Yoshikane 吉兼 道子 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuniの上皮細胞侵入性に対するCFTRの関与について 第251回 徳島医学会学術集会 20150802 6339428/presentations/16422647 Takaaki Shimohata 下畑 隆明 Kido Junko 木戸 純子 Negoro Sachie 根来 幸恵 Hatayama Sho 畑山 翔 Sato Yuri 佐藤 優里 Iba Hitomi 射場 仁美 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 CFTR regulates Campylobacter jejuni invasion in cultured cells 日米医学協力研究会コレラ・細菌性腸管感染症専門部会 20150730 6339428/presentations/16422528 Tadahiko Nakagawa Yoshifumi Takaoka Hironori Tanaka Kumiko Tanaka Tetsuji Takayama Takaaki Shimohata Takashi Uebansou Kazuaki Mawatari Akira Takahashi Masatake Akutagawa Takahiro Emoto Yohsuke Kinouchi Toru Murashima Satoru Yamaji Zenji Tanaka Masahiro Sogabe Toshiya Okahisa Roller Pump Circulation System For Preventing Filter Clogging During Cell-free and Concentrated Ascites Reinfusion Therapy (cart). The 61th Annual Conference of American Society for Artificial Internal Organs(ASAIO) 20150625 6339428/presentations/16422529 Tsunedomi Akari Masamura Akinori Mutsumi Nakahashi Nishisaka Risa Kazuaki Mawatari Takaaki Shimohata Takashi Uebansou Katsuyuki Miyawaki Masatake Akutagawa Yohsuke Kinouchi Akira Takahashi Disinfection system with UVA-LED for hydroponic nutrient solution 6th FEMS Microbiology Congress 20150610 6339428/presentations/16422532 Takaaki Shimohata Negoro Sachie Kido Junko Hatayama Sho Sato Yuri Iba Hitomi Mutsumi Nakahashi Takashi Uebansou Kazuaki Mawatari Akira Takahashi INFECTION OF CAMPYLOBACTER JEJUNI REDUCES CFTR MEDIATED CL- SECRETION IN T-84 6th FEMS Microbiology Congress 20150609 6339428/presentations/16422531 Takashi Uebansou Takaaki Shimohata Iba Hitomi Nishimura Kazuya Taniguchi Yuichi Kazuki Horikawa Mutsumi Nakahashi Kazuaki Mawatari Akira Takahashi COMBINATION BETWEEN A FEW T3SS INJECTISOME AND A LOT EFFECTOR FOR KILLING HOST CELLS ON VIBRIO PARAHAEMOLYTICUS 6th FEMS Microbiology Congress 20150609 6339428/presentations/16422530 Hatayama Sho Takaaki Shimohata Negoro Sachie Sato Yuri Kido Junko Mutsumi Nakahashi Takashi Uebansou Kazuaki Mawatari Akira Takahashi The denudation of lateral part promote Campylobacter jejuni invasion in Caco-2 cells. 6th FEMS Microbiology Congress 20150609 6339428/presentations/16422534 Takashi Uebansou Ai Ohnishi Takaaki Shimohata Mutsumi Nakahashi Kazuaki Mawatari Akira Takahashi Acceptable daily intake levels of sucralose consumption reduces fecal Clostridium cluster 4 in mouse 12th Asian Congress of Nutrition 20150515 6339428/presentations/16422533 Ayumi Yoshimoto Takashi Uebansou Kazuaki Mawatari Mutsumi Nakahashi Takaaki Shimohata Akira Takahashi Low dose antibiotics treatment in the prenatal period affect newborns health in later life 12th Asian Congress of Nutrition 20150515 6339428/presentations/16422649 Junko Kido 木戸 純子 Takaaki Shimohata 下畑 隆明 Sachie Negoro 根来 幸恵 Syo Hatayama 畑山 翔 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 CFTRがCampylobacter jejuniの細胞侵入性へ与える影響 第89回 日本感染症学会総会・学術講演 20150417 6339428/presentations/16422648 Tomoko Yamashita 山下 智子 Kazuaki Mawatari 馬渡 一諭 Junko Kido 木戸 純子 Takaaki Shimohata 下畑 隆明 Akira Takahashi 橋 章 人口透析装置汚染防止を目的とした近紫外発光ダイオード(UVA-LED)による照射殺菌条件の検討 第89回 日本感染症学会総会・学術講演 20150417 6339428/presentations/16422652 Syo Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 Sachie Negoro 根来 幸恵 Yuri Sato 佐藤 優里 Junko Kido 木戸 純子 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuniは腸管上皮の側部より効率的に侵入する 第88回 日本細菌学会総会 20150326 6339428/presentations/16422651 Quang Ngoc Phan Takashi Uebansou Kazuaki Mawatari Takaaki Shimohata Mutsumi Nakahashi Akira Takahashi DNA-binding protein HU coordinates pathogenicity in Vibrio parahaemolyticus 第88回 日本細菌学会総会 20150326 6339428/presentations/16422650 Kazuaki Mawatari 馬渡 一諭 Airi Honjyo 本庄 アイリ Miki Yasui 安井 実希 Syo Hatayama 畑山 翔 Mutsumi Nakahashi 中橋 睦美 Takaaki Shimohata 下畑 隆明 Akira Takahashi 橋 章 腸炎ビブリオのVP2357遺伝子変異は近紫外線(UVA)照射耐性獲得に寄与する 第88回 日本細菌学会総会 20150326 6339428/presentations/16422766 Yuri Sato 佐藤 優里 Takaaki Shimohata 下畑 隆明 Sachie Negoro 根来 幸恵 Syo Hatayama 畑山 翔 Junko Kido 木戸 純子 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Nagakatsu Harada 原田 永勝 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni感染とAutophagyの関連について 第7回 日本カンピロバクター研究会 総会 20141211 6339428/presentations/16422765 Takaaki Shimohata 下畑 隆明 Junko Kido 木戸 純子 Sachie Negoro 根来 幸恵 Syo Hatayama 畑山 翔 Yuri Sato 佐藤 優里 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 CFTRはCampylobacter jejuniの侵入抑制に寄与する 第7回 日本カンピロバクター研究会 総会 20141211 6339428/presentations/16422655 Junko Kido 木戸 純子 Takaaki Shimohata 下畑 隆明 Sachie Negoro 根来 幸恵 Syo Hatayama 畑山 翔 Yuri Sato 佐藤 優里 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuniによる細胞侵入とCFTRの関連について 第67回 日本細菌学会中国・四国支部総会 20141004 6339428/presentations/16422654 Akari Tsunedomi 常冨 愛香里 Mutsumi Nakahashi 中橋 睦美 Risa Nishisaka 西坂 理沙 Kazuaki Mawatari 馬渡 一諭 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Katsuyuki Miyawaki 宮脇 克行 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 水耕栽培用養液の還流型殺菌システムの開発 第67回 日本細菌学会中国・四国支部総会 20141004 6339428/presentations/16422653 Phan Quang Ngoc Takashi Uebansou Kazuaki Mawatari Takaaki Shimohata Mutsumi Nakahashi Akira Takahashi DNA-binding protein HU coordinate pathogenicity in Vibrio parahaemolyticus 第67回 日本細菌学会中国・四国支部総会 20141004 6339428/presentations/16422657 Teppei Hoshiyama 星山 哲平 Masatake Akutagawa 芥川 正武 Takahiro Emoto 榎本 崇宏 Mutsumi Nakahashi 中橋 睦美 Marhio Hayashida 林田 麻里王 Akira Takahashi ?橋 章 Kazuaki Mawatari 馬渡 一諭 Toshitaka Ikehara 池原 敏孝 Shinsuke Konaka 小中 信典 Yohsuke Kinouchi 木内 陽介 UV及び可視光照射に対する腸炎ビブリオの遺伝子発現解析 腸炎ビブリオを紫外線および可視光に曝露した際，遺伝子発現がどのように変化するかについて，遺伝子チップを用いて網羅的に実験・解析を行っている．その結果，UV-A領域の光を照射した際，酸化修復に関する遺伝子の働きが抑えられること，緑色の光を照射した際に鞭毛関連の遺伝子が活性化することなどが示された． Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20140913 6339428/presentations/16422656 矢野 佑樹 Masatake Akutagawa 芥川 正武 Takahiro Emoto 榎本 崇宏 Shinsuke Konaka 小中 信典 Shinsuke Konaka 小中 信典 Yohsuke Kinouchi 木内 陽介 Takaaki Shimohata 下畑 隆明 Akira Takahashi 橋 章 Emiko Yasuno 安野 恵実子 有限要素法を用いたマウス腸管のインピーダンス測定方法の検討 クローン病などの腸の疾患で腸管の電気インピーダンスが健常者と有病者とで異なることが知られている．本研究では電気インピーダンスを用いた腸疾患の診断を目的として，マウスの腸管インピーダンスを測定する際の電極形状について，有限要素法を用いて基礎研究を行っている． Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20140913 6339428/presentations/16422660 Hatayama Syo 畑山 翔 Takaaki Shimohata 下畑 隆明 Negoro Sachie 根来 幸恵 Sato Yuri 佐藤 優里 Kido Junko 木戸 純子 Mutsumi Nakahashi 中橋 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni 感染による腸管上皮細胞のイオン輸送に関する検討 第249回徳島医学会学術集会 20140727 6339428/presentations/16422659 Phan Quang Ngoc Takashi Uebansou Kazuaki Mawatari Takaaki Shimohata Mutsumi Aihara Akira Takahashi DNA-binding protein HU coordinates pathogenicity in Vibrio parahaemolyticus. 第249回徳島医学会学術集会 20140727 6339428/presentations/16422658 Honjo Airi 本庄 アイリ Kazuaki Mawatari 馬渡 一諭 Maetani Miki 前谷 実希 Iwamoto Natsumi 岩本 夏実 Yamashita Tomoko 山下 智子 Mutsumi Nakahashi 中橋 睦美 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Akira Takahashi 橋 章 Anti-sigma factor VP2357の遺伝子変異はVibrio parahaemolyticusの近紫外線(UVA)耐性獲得に関与する 第249回徳島医学会学術集会 20140727 6339428/presentations/16422661 Teppei Hoshiyama 星山 哲平 Masatake Akutagawa 芥川 正武 Takahiro Emoto 榎本 崇宏 Mutsumi Nakahashi 中橋 睦美 Marhio Hayashida 林田 麻里王 Akira Takahashi ?橋 章 Kazuaki Mawatari 馬渡 一諭 Toshitaka Ikehara 池原 敏孝 Shinsuke Konaka 小中 信典 Yohsuke Kinouchi 木内 陽介 Gene expression analysis of Vibrio parahaemolyticus for the light irradiation 異なる波長の光照射に対する腸炎ビブリオの遺伝子発現解析 Effect of the light irradiation brings to the bacteria has been reported. For example, the sterilizing effect is obtained by the ultraviolet (UV) irradiation. Bacteria is known that they have photoreceptors in the visible light irradiation. Thus, it seems that effect of light irradiation depends on their wavelength. In this study, gene expression analysis was performed against Vibrio parahaemolyticus in order to elucidate the effect of the difference in wavelengths. As a result of them, Flagellar migration function of Vibrio parahaemolyticus was activated when it was irradiated with light of 520nm in wavelength. 本研究は細菌へどのような光を照射すると， どのような機能が活性されるかについて考察するものである． 現在， 光照射が細菌にもたらす影響が多数報告されており， 例えば紫外(UV)照射を行う事で殺菌効果が得られ， 可視光照射では光受容体という機能の存在が示されている． 本研究では， このような異なる波長の光照射に対する細菌への影響を， 遺伝子発現解析により考察した． その結果， UV及び可視光の各波長の光照射時について変動の見られた腸炎ビブリオ遺伝子を特定する事ができた． また， 520nmの光を腸炎ビブリオに照射すると， 腸炎ビブリオの鞭毛遊走機能が活性化される可能性が示唆された． Institute of Electronics, Information and Communication Engineers 電子情報通信学会 IEICE Technical Report (MBE) 電子情報通信学会技術研究報告(MEとバイオサイバネティックス) 20140726 6339428/presentations/16422664 Sachie Negoro 根耒 幸恵 Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Mari Matsumoto 松本 麻里 Yuri Sato 佐藤 優里 Mutsumi Aihara 粟飯原 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Suppression of CFTR-mediated Cl- transport in Campylobacter jejuni infection Campylobacter jejuni 感染はCFTR機能を抑制する 第68回日本栄養・食糧学会大会 20140531 6339428/presentations/16422663 本庄 アイリ Kazuaki Mawatari 馬渡 一諭 前谷 実希 常冨 愛香里 畑山 翔 Mutsumi Aihara 粟飯原 睦美 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Akira Takahashi 橋 章 Upregulation of katG gene, VPA0768, induces ultraviolet-A (UVA) resistance in Vibrio parahaemolyticus Vibrio parahaemolyticusのkatGgene, VPA0768発現上昇は近紫外線(UVA)耐性獲得に関与する 第68回日本栄養・食糧学会大会 20140531 6339428/presentations/16422662 Akari Tsunedomi 常冨 愛香里 正村 彰規 Mutsumi Aihara 粟飯原 睦美 Kazuaki Mawatari 馬渡 一諭 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Masachika Ishizaki 石崎 仁愛 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 近紫外線による水耕栽培用培養液の殺菌法の開発 第68回日本栄養・食糧学会大会 20140531 6339428/presentations/16422535 Takaaki Shimohata Sachie Negoro Sho Hatayama Yuri Sato Hitomi Iba Mutsumi Aihara Takashi Uebansou Kazuaki Mawatari Akira Takahashi Campylobacter jejuni Infection Reduces Cftr Mediated Cl- Secretion In T-84 American Society for Microbiology 114th General Meeting 20140500 6339428/presentations/16422665 Kazuaki Mawatari 馬渡 一諭 Airi Honjoh 本庄 アイリ Akari Tsunedomi 常冨 愛香里 Sho Hatayama 畑山 翔 Mutsumi Aihara 粟飯原 睦美 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Akira Takahashi 橋 章 近紫外線(UVA)照射により酸化される腸炎ビブリオタンパク質の同定 第87回日本細菌学会総会 20140327 6339428/presentations/16422666 Takaaki Shimohata 下畑 隆明 Sachie Negoro 根耒 幸恵 Sho Hatayama 畑山 翔 Yuri Sato 佐藤 優里 Mutsumi Aihara 粟飯原 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni infection reduces Cl- secretion in T-84 Campylobacter jejuni感染はT84細胞におけるCl-分泌を抑制する 第87回日本細菌学会総会 20140326 6339428/presentations/16422536 Mutsumi Aihara Takashi Uebansou Takaaki Shimohata Kazuaki Mawatari Masatake Akutagawa Yohsuke Kinouchi Akira Takahashi Simultaneous irradiation with different wavelengths of ultraviolet light has synergistic bactericidal effect on Vibrio parahaemolyticus 48th US-Japan Cooperative Medical Sciences Program Conference on cholera and Other Bacterial Enteric Infections 20140200 6339428/presentations/16422667 Takaharu Kudoh 工藤 隆治 Kenji Fujisawa 藤澤 健司 Keiko Kudoh 工藤 景子 Masako Fujioka-Kobayashi 小林 真左子 Nobuyuki Kuribayashi 栗林 伸行 Natsumi Takamaru 丸 菜都美 Go Ohe 大江 剛 Daisuke Uchida 内田 大亮 Akira Takahashi ?橋 章 Tetsuya Tamatani 玉谷 哲也 Hirokazu Nagai 永井 宏和 Hitoshi Ikushima 生島 仁史 Youji Miyamoto 宮本 洋二 当科における強度変調放射線治療(IMRT)の経験 第12回中国四国口腔癌研究会学術講演会 20131115 6339428/presentations/16422669 Airi Honjoh 本庄 アイリ Miyuki Edagawa 枝川 美幸 Kazuaki Mawatari 馬渡 一諭 Miki Maetani 前谷 実希 Mutsumi Aihara 粟飯原 睦美 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Maki Uwate 上手 麻希 Hideaki Maseda 間世田 英明 Akira Takahashi 橋 章 トランスポゾン挿入変異ライブラリを用いた腸炎ビブリオの近紫外線関連遺伝子の探索 第66回 日本細菌学会中国・四国支部総会 20131012 6339428/presentations/16422668 Mario Hayashida 林田 麻里王 Mutsumi Aihara 粟飯原 睦美 Takashi Uebansou 上番増 喬 Takaaki Shimohata 下畑 隆明 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi ?橋 章 Teppei Hoshiyama 星山 哲平 Masatake Akutagawa 芥川 正武 Takahiro Emoto 榎本 崇宏 Yohsuke Kinouchi 木内 陽介 紫外線・可視光照射により変動する微生物遺伝子の網羅的解析 第66回 日本細菌学会中国・四国支部総会 20131012 6339428/presentations/16422671 Ishizaki Masachika 石崎 仁愛 Masatake Akutagawa 芥川 正武 Akira Takahashi ?橋 章 Yohsuke Kinouchi 木内 陽介 Takahiro Emoto 榎本 崇宏 Toshitaka Ikehara 池原 敏孝 Shinsuke Konaka 小中 信典 Derivation of the model equation, that represent sterilization transition of UV-A UV-A殺菌推移を表すモデル化に関する研究 UV-Aを用いた殺菌について，UV-A照射量と菌数の関係を表すモデル式を提案し，実験結果と比較している．実験は150uL，0.7L，100Lの各容量のプレートおよびステンレスタンクについて，大腸菌を一定濃度で入れた菌液を用いて近紫外線を照射して菌数の時間的推移を測定した．その結果，菌数の時間推移の傾向をモデル式で表すことができた． 2013 Shikoku-section Joint Convention Record of the Institutes of Electrical and Related Engineers 平成25年度電気関係学会四国支部連合大会講演論文集 20130921 6339428/presentations/16422670 Teppei Hoshiyama 星山 哲平 Masatake Akutagawa 芥川 正武 Takahiro Emoto 榎本 崇宏 Shinsuke Konaka 小中 信典 Yohsuke Kinouchi 木内 陽介 Mutsumi Aihara 粟飯原 睦美 Mario Hayashida 林田 麻里王 Akira Takahashi 橋 章 Gene expression analysis of Vibrio parahaemolyticus against UV irradiation UV照射に対する腸炎ビブリオの遺伝子発現解析 腸炎ビブリオを紫外線に曝露した際，遺伝子発現がどのように変化するかについて，遺伝子チップを用いて網羅的に実験・解析を行っている．UV-A，UV-B，UV-Cそれぞれの代表的な波長について，腸炎ビブリオにUV照射した後に解析を行い，各波長ごとの遺伝子発現の違いについて調査した． 2013 Shikoku-section Joint Convention Record of the Institutes of Electrical and Related Engineers 平成25年度電気関係学会四国支部連合大会講演論文集 20130921 6339428/presentations/16422537 Sachie Negoro Takaaki Shimohata Syo Hatayama Mari Matsumoto Yuri Sato Mutsumi Aihara Takashi Uebansou Kazuaki Mawatari Akira Takahashi Suppression of CFTR-mediated Cl- transport in Campylobacter jejuni infection International Symposium Hannover - Tokushima Research Communication 20130811 6339428/presentations/16422672 Mutsumi Aihara Takashi Uebansou Takaaki Shimohata Kazuaki Mawatari Akira Takahashi Synergistic bactercidal effect of Ultraviolet light with a combination of different 日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会 20130808 6339428/presentations/16422678 Sho Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 根来 幸恵 Mari Matsumoto 松本 麻里 Yuri Sato 佐藤 優里 Mutsumi Aihara 粟飯原 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 食中毒起因菌カンピロバクターによる下痢の機序に関する研究 第247回徳島医学会学術集会 20130804 6339428/presentations/16422677 根来 幸恵 Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Mari Matsumoto 松本 麻里 Yuri Sato 佐藤 優里 Mutsumi Aihara 粟飯原 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni 感染によるCl‐輸送チャネルの活性変化 第247回徳島医学会学術集会 20130804 6339428/presentations/16422676 Airi Honjoh 本庄 アイリ Kazuaki Mawatari 馬渡 一諭 Mutsumi Aihara 粟飯原 睦美 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Akira Takahashi 橋 章 VP0221とVPA1604は腸炎ビブリオの莢膜多糖類を調節し，抗菌ペプチドと補体耐性に働く 第247回徳島医学会学術集会 20130804 6339428/presentations/16422675 Shoko Asada 浅田 翔子 Hitomi Iba 射場 仁美 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 腸炎ビブリオの3型分泌装置遺伝子発現は細胞接着により誘導される 第247回徳島医学会学術集会 20130804 6339428/presentations/16422674 Akari Tsunedomi 常冨 愛香里 Mutsumi Aihara 粟飯原 睦美 Masachika Ishizaki 石崎 仁愛 Takashi Uebansou 上番増 喬 Takaaki Shimohata 下畑 隆明 Masatake Akutagawa 芥川 正武 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 正村 彰規 UVA-LED を用いた水耕栽培用養液の殺菌 第247回徳島医学会学術集会 20130804 6339428/presentations/16422673 Mario Hayashida 林田 麻里王 Mutsumi Aihara 粟飯原 睦美 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi ?橋 章 Teppei Hoshiyama 星山 哲平 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 DNA マイクロアレイを用いた微生物遺伝子の網羅的解析 第247回徳島医学会学術集会 20130804 6339428/presentations/16422768 Sho Hatayama 畑山 翔 Takaaki Shimohata 下畑 隆明 根来 幸恵 Mari Matsumoto 松本 麻里 Yuri Sato 佐藤 優里 Mutsumi Aihara 粟飯原 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuniによる下痢誘発機構の検討 第6回日本カンピロバクター研究会 20130726 6339428/presentations/16422767 根来 幸恵 Takaaki Shimohata 下畑 隆明 Sho Hatayama 畑山 翔 Mari Matsumoto 松本 麻里 Yuri Sato 佐藤 優里 Mutsumi Aihara 粟飯原 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni感染はCFTR機能を抑制する 第6回日本カンピロバクター研究会 20130726 6339428/presentations/16422538 Takashi Uebansou Hitomi Iba Shoko Asada Takaaki Shimohata Mutsumi Aihara Kazuaki Mawatari Akira Takahashi Regulation and effects of VP1671 (VscQ) on virulence of Vibrio parahaemolyticus 5th FEMS Microbiology Congress 20130724 6339428/presentations/16422539 Mutsumi Aihara Takaaki Shimohata Takashi Uebansou Kazuaki Mawatari Akira Takahashi Synergistic bactericidal effect of ultraviolet light with a combination of different wavelength 5th FEMS Microbiology Congress 20130723 6339428/presentations/16422540 Kazuaki Mawatari Tam Thanh Thi Le Miki Maetani Tomomi Yamamoto Airi Honjo Mutsumi Aihara Takaaki Shimohata Takashi Uebansou Akira Takahashi VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress 5th FEMS Microbiology Congress 20130722 6339428/presentations/16422541 Teppei Hoshiyama Masatake Akutagawa Mutsumi Aihara Mario Hayashida Akira Takahashi Takahiro Emoto Shinsuke Konaka Yohsuke Kinouchi Gene expression analysis of Vibrio parahaemolyticus for UV irradiation 腸炎ビブリオを紫外線に曝露した際，遺伝子発現がどのように変化するかについて，遺伝子チップを用いて網羅的に実験・解析を行っている．UV-A，UV-B，UV-Cそれぞれの代表的な波長について，腸炎ビブリオにUV照射した後に解析を行い，各波長ごとの遺伝子発現の違いについて調査した． BioEM2013 20130610 6339428/presentations/16422679 根来 幸恵 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni 感染とCl-輸送の変化 第87回日本感染症学会総会・学術講演会 20130606 6339428/presentations/16422680 Takaaki Shimohata 下畑 隆明 根来 幸恵 Sho Hatayama 畑山 翔 Mari Matsumoto 松本 麻里 Mutsumi Aihara 粟飯原 睦美 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Campylobacter jejuni感染によるクロライド分泌の変化 第86回日本細菌学会総会 20130318 6339428/presentations/16422542 Kazuaki Mawatari Tam Thanh Thi Le Miki Maetani Tomomi Yamamoto Mutsumi Aihara Takaaki Shimohata Takashi Uebansou Akira Takahashi VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress US-Japan Cooperative Medical Science Program, 47th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections 20121200 6339428/presentations/16422543 Takashi Uebansou Hitomi Iba Shoko Asada Takaaki Shimohata Akira Takahashi REGULATION AND EFFECTS OF VP1671(VSCQ) ON VIRULENCE Of VIBRIO PARAHAEMOLYTICUS International Symposium on Single cell Analysis 20121127 6339428/presentations/16422682 Kazuaki Mawatari 馬渡 一諭 Kana Kurokawa 黒川 香菜 Airi Honjoh 本庄 アイリ Mami Fujii 藤井 麻未 Tomomi Yamamoto 山本 智実 Miki Maetani 前谷 実希 Mutsumi Aihara 粟飯原 睦美 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Akira Takahashi 橋 章 Vibrio parahaemolyticusを用いたUVA紫外線照射による殺菌機構の解析 第65回日本細菌学会・中国・四国支部総会 20121000 6339428/presentations/16422681 Shoko Asada 浅田 翔子 Hitomi Iba 射場 仁美 Takaaki Shimohata 下畑 隆明 Takashi Uebansou 上番増 喬 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 Vibrio parahaemolyticusの病原因子 TTSS1遺伝子発現は細胞接着によって誘導される 第65回日本細菌学会・中国・四国支部総会 20121000 6339428/presentations/16422683 Masachika Ishizaki 石崎 仁愛 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Takahiro Emoto 榎本 崇宏 Shinsuke Konaka 小中 信典 Akira Takahashi 橋 章 Toshitaka Ikehara 池原 敏孝 Modeling of sterilization using UV-A UVAによる殺菌のモデル化に関する研究 UV-Aを用いた殺菌について，UV-A照射量と菌数の関係を表すモデル式を提案し，実験結果と比較している．0.7Lステンレスタンクについて，大腸菌を一定濃度で入れた菌液を用いて近紫外線を照射して菌数の時間的推移を測定した．その結果，菌数の時間推移の傾向をモデル式で表すことができた． Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20120929 6339428/presentations/16422684 Tam Thanh Thi Le Kazuaki Mawatari Mutsumi Aihara Takaaki Shimohata Takashi Uebansou Akira Takahashi VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress 日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会 20120800 6339428/presentations/16422545 Toshitaka Ikehara Mutsumi Aihara Zehong Su Akira Takahashi Masatake Akutagawa Yohsuke Kinouchi Effects of UVA radiation on growth of RAW 264.7 cells マウス由来のRAW 264.7細胞に対してUVAを照射した際の増殖の違いについて検討した． 34th Annual Conference of the bioelectromagnetics society (34 BEMS) (Abstract) 20120620 6339428/presentations/16422544 Masachika Ishizaki Yusuke Manabe Mutsumi Aihara Akira Takahashi Masatake Akutagawa Takahiro Emoto Yohsuke Kinouchi Toshitaka Ikehara Improvement of a pipe type UVA-LED sterilizer using a condenser lens UV-Aを用いた殺菌について，UV-A照射量と菌数の関係を表すモデル式を提案し，実験結果と比較している．0.7Lステンレスタンクについて，大腸菌を一定濃度で入れた菌液を用いて近紫外線を照射して菌数の時間的推移を測定した．その結果，菌数の時間推移の傾向をモデル式で表すことができた． 34th Annual Conference of the bioelectromagnetics society (34 BEMS) (Abstract) 20120620 6339428/presentations/16422547 Mutsumi Aihara Kazuaki Mawatari Takaaki Shimohata Akira Takahashi Bactericidal Effect of Ultraviolet Light with a Combination of Different Wavelengths 112th General Meeting of the American Society for Microbiology 20120600 6339428/presentations/16422546 Kazuaki Mawatari Yuka Isumi Minami Hirayama Miki Maetani Tomomi Yamamoto Sayaka Hayashida Hitomi Iba Mutsumi Aihara Takaaki Shimohata Akira Takahashi OtnA-OtnB modulate capsular polysaccharide and polymyxin B resistance in Vibrio parahaemolyticus 112th General Meeting of the American Society for Microbiology 20120600 6339428/presentations/16422686 Tomomi Yamamoto 山本 智実 Kazuaki Mawatari 馬渡 一諭 Sayaka Hayashida 林田 沙也加 Takaaki Shimohata 下畑 隆明 Akira Takahashi 橋 章 発光ダイオードによる近紫外線照射(UVA-LED)によるウイルスの不活化 第86回日本感染症学会総会・学術講演会 20120426 6339428/presentations/16422685 Sayaka Hayashida 林田 沙也加 Takaaki Shimohata 下畑 隆明 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 UVA-LEDと塩素を併用した水殺菌法の開発 第86回日本感染症学会総会・学術講演会 20120426 6339428/presentations/16422770 Yusuke Manabe 真鍋 佑輔 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Toshitaka Ikehara 池原 敏孝 Akira Takahashi ?橋 章 Shinsuke Konaka 小中 信典 Takahiro Emoto 榎本 崇宏 石崎 仁愛 近紫外線が細菌に与える影響に関する研究 波長365nmのUVA-LEDを用いた水殺菌装置について検討した．殺菌容器は内径22mmから56mm，深さ50mmから150mmのステンレス製の円筒容器を製作し，上部からUV-LEDを照射する構造とした．またLEDから照射されたUVを有効に利用するためにフレネルレンズを用いた．非病原性大腸菌を用いた実験で殺菌効果を確認した． LED総合フォーラム2012in徳島論文集 20120420 6339428/presentations/16422769 Masachika Ishizaki 石崎 仁愛 Yusuke Manabe 真鍋 佑輔 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Toshitaka Ikehara 池原 敏孝 Shinsuke Konaka 小中 信典 Akira Takahashi 橋 章 Takahiro Emoto 榎本 崇宏 UV-A(近紫外線)照射の殺菌効果に関するモデル化 UV-Aを用いた殺菌について，UV-A照射量と菌数の関係を表すモデル式を提案し，実験結果と比較している．0.7Lステンレスタンクについて，大腸菌を一定濃度で入れた菌液を用いて近紫外線を照射して菌数の時間的推移を測定した．その結果，菌数の時間推移の傾向をモデル式で表すことができた． LED総合フォーラム2012in徳島論文集 20120420 6339428/presentations/16422687 Kazuaki Mawatari 馬渡 一諭 井角 友香 平山 みなみ 前谷 実希 山本 智実 林田 沙也加 射場 仁美 Mutsumi Aihara 粟飯原 睦美 Takaaki Shimohata 下畑 隆明 Akira Takahashi 橋 章 Vibrio parahaemolyticus capsular polysaccharide is modulated by two pathways, VP0220-1 and VPA1602-4 第85回日本細菌学会総会 20120329 6339428/presentations/16422688 Takaaki Shimohata 下畑 隆明 射場 仁美 根来 幸恵 浅田 翔子 Mutsumi Aihara 粟飯原 睦美 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 VP1680 induces IL-8 secretion in Vibrio parahaemolyticus infection 第85回日本細菌学会総会 20120327 6339428/presentations/16422689 Takaaki Shimohata 下畑 隆明 射場 仁美 根来 幸恵 浅田 翔子 Mutsumi Aihara 粟飯原 睦美 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 腸炎ビブリオT3SS1におけるトランスロコンの解析 第64回日本細菌学会中国・四国支部総会 20111023 6339428/presentations/16422690 林田 沙也加 Mutsumi Aihara 粟飯原 睦美 Takaaki Shimohata 下畑 隆明 Kazuaki Mawatari 馬渡 一諭 Masatake Akutagawa 芥川 正武 木ノ内 陽介 Akira Takahashi 橋 章 水中の細菌に対するUVA-LEDと塩素との併用殺菌効果 第64回日本細菌学会中国・四国支部総会 20111022 6339428/presentations/16422692 真鍋 祐矢 中川 亮佑 Su Zehong 前谷 実希 Kenji Teranishi 寺西 研二 Naoyuki Shimomura 下村 直行 Akira Takahashi 橋 章 病原性細菌の遺伝子発現におけるパルス電界の影響に関する研究 平成23年度電気関係学会四国支部連合大会講演論文集 20110923 6339428/presentations/16422691 Yusuke Manabe 真鍋 佑輔 Tsuyoshi Okahisa 岡久 強志 Masayuki Yamato 大和 正幸 Akira Takahashi ?橋 章 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Takahiro Emoto 榎本 崇宏 Improvement of a Pipe type UVA-LED Sterilizer Using a Condenser Lens UVA-LED を用いたパイプ型殺菌装置における レンズの効果 UV-LEDを用いた新しい殺菌装置の開発を目指し，放射照度分布と殺菌効果の関係を研究している．波長365nmのUVを発光するUVA-LEDを用いてタンク内の水を殺菌する装置を製作した．2種類のタンクを用いて，フレネルレンズを装着した場合と装着していない場合で殺菌実験を行いと殺菌効果への影響を検討した． Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20110923 6339428/presentations/16422693 Kazuaki Mawatari 馬渡 一諭 Yuka Isumi Minami Hirayama Miki Maetani Tomomi Yamamoto Sayaka Hayashida Hitomi Iba Mutsumi Aihara 粟飯原 睦美 Takaaki Shimohata 下畑 隆明 Akira Takahashi 橋 章 VP0220-0221 and VPA1602-1604 modulate capsular polysaccharides in Vibrio parahaemolyticus 日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会 20110803 6339428/presentations/16422548 Hitomi Iba Takaaki Shimohata Masayuki Yamato Kazuaki Mawatari Akira Takahashi The effect of VP1671 (vscQ) on virulence of Vibrio parahaemolyticus 4th FEMS Microbiology Congress 20110629 6339428/presentations/16422549 Kazuaki Mawatari Yumi Yoneda Manami Honda Hitomi Iba Mutsumi Aihara Zehong Su Takaaki Shimohata Masayuki Yamato Akira Takahashi VPA1604, a protein containing phosphorylated tyrosine, modulates high molecular weight capsular polysaccharide in Vibrio parahaemolyticus 4th FEMS Microbiology Congress 20110628 6339428/presentations/16422771 Tomohiro Oshita 大下 知洋 岡久 強志 真鍋 佑輔 Masayuki Yamato 大和 正幸 Takahiro Emoto 榎本 崇宏 Masatake Akutagawa 芥川 正武 Akira Takahashi 橋 章 Yohsuke Kinouchi 木内 陽介 光殺菌効果の波長依存性に関する研究 LED総合フォーラム2011in徳島論文集 20110625 6339428/presentations/16422550 Yuya Manabe Ryosuke Nakagawa Su Zhehong Miki Maetani Kenji Teranishi Naoyuki Shimomura Akira Takahashi Influences of Pulsed Electric Fields on the Gene Expression of Pathogenic Bacteria IEEE Proceedings of the 18th IEEE International Pulsed Power Conference 20110622 6339428/presentations/16422694 Takaaki Shimohata 下畑 隆明 廉 馨 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi 橋 章 腸炎ビブリオ感染は腸管上皮細胞においてMAPKsを活性化しIL-8の分泌を誘発する 第85回日本感染症学会総会・学術講演会 20110422 6339428/presentations/16422551 Kazuaki Mawatari Yumi Yoneda Manami Honda Hitomi Iba Mutsumi Aihara Zehong Su Takaaki Shimohata Masayuki Yamato Akira Takahashi VPA1604, a protein tyrosine kinase, modulates high molecular weight capsular polysaccharide in Vibrio parahaemolyticus US-Japan Cooperative Medical Science Program, 45th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections Panel 20101200 6339428/presentations/16422697 Takaaki Shimohata 下畑 隆明 中野 政之 Kazuaki Mawatari 馬渡 一諭 Masayuki Yamato 大和 正幸 Akira Takahashi 橋 章 腸炎ビブリオエフェクタータンパク質VP1680 はIL-8 の分泌を誘発する 第63回日本細菌学会・中国・四国支部総会 20101000 6339428/presentations/16422696 Mutsumi Aihara 粟飯原 睦美 Masayuki Yamato 大和 正幸 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi ?橋 章 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 異なる紫外線波長の併用による殺菌効果 第63回日本細菌学会・中国・四国支部総会 20101000 6339428/presentations/16422695 Kazuaki Mawatari 馬渡 一諭 米田 由美 本田 真奈美 射場 仁美 Mutsumi Aihara 粟飯原 睦美 Takaaki Shimohata 下畑 隆明 蘇 澤紅 Masayuki Yamato 大和 正幸 Akira Takahashi 橋 章 Vibrio parahaemolyticus のチロシンキナーゼVPA1604 は高分子量型莢膜多糖類(HMW-CPS)形成を調節する 第63回日本細菌学会・中国・四国支部総会 20101000 6339428/presentations/16422699 Tomohiro Oshita 大下 知洋 Keiko Shintani 新谷 佳子 Mai Katayama 片山 麻衣 Masayuki Yamato 大和 正幸 Masatake Akutagawa 芥川 正武 Akira Takahashi ?橋 章 Shinsuke Konaka 小中 信典 Yohsuke Kinouchi 木内 陽介 An investigation of the wavelength dependence of the sterilization effect 殺菌効果の波長依存性に関する研究 Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20100925 6339428/presentations/16422698 Tsuyoshi Okahisa 岡久 強志 Yusuke Manabe 真鍋 佑輔 Masayuki Yamato 大和 正幸 Akira Takahashi ?橋 章 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 UVA-LEDfor sterilizingwater inawater tank UV-LEDを用いたタンク型水殺菌装置 Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20100925 6339428/presentations/16422700 Kazuaki Mawatari Yumi Yoneda Manami Honda Hitomi Iba Mutsumi Aihara Zehong Su Takaaki Shimohata Masayuki Yamato Akira Takahashi VPA1604, a protein tyrosine kinase, modulates high molecular weight capsular polysaccharide in Vibrio parahaemolyticus 日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会 20100700 6339428/presentations/16422554 Tsuyosi Okahisa Masayuki Yamato Akira Takahashi Masatake Akutagawa Takahiro Emoto Yohsuke Kinouchi UVA-LED for sterilizing water in a water tank The Bioelectromagnetics Society Proceedings of The Bioelectromagnetics Society 32nd Annual Meeting 20100617 6339428/presentations/16422553 Yuhsuke Manabe Takahiro Emoto Masatake Akutagawa Akira Takahashi Yohsuke Kinouchi Simulation of UVA-LED irradiance distribution The Bioelectromagnetics Society Proceedings of The Bioelectromagnetics Society 32nd Annual Meeting 20100617 6339428/presentations/16422552 Miku Maeda Yohsuke Kinouchi Akira Takahashi Takahiro Emoto Masatake Akutagawa Xin Lian Novel sterilization device using 265nm UV-LED for escherichia coli The Bioelectromagnetics Society Proceedings of The Bioelectromagnetics Society 32nd Annual Meeting 20100617 6339428/presentations/16422555 Tomohiro Oshita Keiko Shintani Mai Katayama Takahiro Emoto Masatake Akutagawa Akira Takahashi Yohsuke Kinouchi An investigation of the sterilization effect of 385nm UVA-LED The Bioelectromagnetics Society Proceedings of The Bioelectromagnetics Society 32nd Annual Meeting 20100615 6339428/presentations/16422556 Takaaki Shimohata Masayuki Nakano Lian Xin Kazuaki Mawatari Masayuki Yamato Akira Takahashi Vibrio parahaemolyticus modulate IL-8 secretion through dual pathway 110th General Meeting of the American Society for Microbiology 20100600 6339428/presentations/16422557 Ryosuke Nakagawa Kazuki Siroyama Su Zehong Kenji Teranishi Naoyuki Shimomura Akira Takahashi Effects of Pulse electric Fields to the Pathogenic Bacteria The pulsed power technology is gaining ground on applications to biological field such as sterilization with pulse electric fields or discharges and induction of the apoptosis with pulse electric fields. Under the circumstances, the pulse electric fields clearly have some influence on organism. To figure out the influence and mechanism, we conducted an experiment. The pulse electric fields were applied to the pathogenic bacteria, and manifestation of RNAs which affected pathogenicity was examined. A system was developed to apply the pulsed electric fields to bacteria. The pulsed power generator, which consists of a Blumlein-type pulse forming network (B-PFN), outputs pulses with width of 100 ns in the case of 5 L-C stages. The electrodes chamber was filled with the solution (2 ml) with bacteria. The separation of the electrodes was 5 mm and approximately uniform electric field was applied to the solution. The manifestation of RNAs was analyzed with the method of polymerase chain reaction, PCR Vibrio parahaemolyticus (RIMD 2210633) was selected to examine. The manifestations of RNAs as VP1699, VP1680, VPA1321, VPA1327, VPA1346, TDH, and RNA16S were examined with the PCR method. Although RNA16S does not become an index for pathogenicity, evaluation of RNA16S implies a survival ratio of the bacteria. The initial density of bacteria solution was regulated to be 109 /ml. The peak voltage was approximately 6, 12 kV and the number of applied pulses was 1000 for each sample. The tendency of increase in manifestation was found by the application of pulse electric fields for VP1699 and VP1680 with relation of TTSS(1). IEEE Proceedings of the 2010 IEEE Power Modulators and High-Voltage Conference 20100526 6339428/presentations/16422702 Takaaki Shimohata 下畑 隆明 中野 政之 廉 馨 Kazuaki Mawatari 馬渡 一諭 Masayuki Yamato 大和 正幸 Akira Takahashi 橋 章 腸炎ビブリオはMAPKsを介したIL-8の分泌を誘発する 第64回日本栄養・食糧学会大会 20100500 6339428/presentations/16422701 射場 仁美 Takaaki Shimohata 下畑 隆明 Kazuaki Mawatari 馬渡 一諭 Masayuki Yamato 大和 正幸 Takamasa Ohnishi 大西 隆仁 Akira Takahashi 橋 章 腸管ループを用いた腸炎ビブリオ誘発下痢機構の検討 第64回日本栄養・食糧学会大会 20100500 6339428/presentations/16422775 Masayuki Yamato 大和 正幸 Haruka Ryouji 料治 春華 Akira Takahashi ?橋 章 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 UVA LEDによる水殺菌 LED総合フォーラム論文集 20100417 6339428/presentations/16422774 Tsuyoshi Okahisa 岡久 強志 Yusuke Manabe 眞鍋 佑輔 Masayuki Yamato 大和 正幸 Akira Takahashi ?橋 章 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 UVA-LED装置を用いた水殺菌装置の開発 LED総合フォーラム論文集 20100417 6339428/presentations/16422773 Xin Lian Miku Maeda Masayuki Yamato Kazuaki Mawatari Masatake Akutagawa Yutaka Nakaya Yohsuke Kinouchi Akira Takahashi New UVA-LED surface sterilization system for vegetables LED総合フォーラム論文集 20100417 6339428/presentations/16422772 Mostafa Gadelmoula Xin Lian Akiko Hamamoto Kazuaki Mawatari Masayuki Yamato Masatake Akutagawa Yutaka Nakaya Yohsuke Kinouchi Akira Takahashi UVA-LED suitability for air disinfection LED総合フォーラム論文集 20100417 6339428/presentations/16422703 中川 亮佑 城山 和己 Su Zehong Kenji Teranishi 寺西 研二 Naoyuki Shimomura 下村 直行 Akira Takahashi 橋 章 病原性細菌へのパルス高電界印加効果 平成22年電気学会全国大会講演論文集 20100317 6339428/presentations/16422705 Kazuaki Mawatari Yukina Takahashi Nami Morikawa Takaaki Shimohata Akiko Hamamoto Zehong Su Masayuki Yamato Akira Takahashi VPA1604, a protein tyrosine kinase, modulate HMW K6-polysaccharide in Vibrio parahaemolyticus 第83回日本細菌学会総会 20100300 6339428/presentations/16422704 Takaaki Shimohata Masayuki Nakano Lian Xin Kazuaki Mawatari Masayuki Yamato Akira Takahashi Vibrio parahaemolyticus modulate IL-8 secretion through dual pathway 第83回日本細菌学会総会 20100300 6339428/presentations/16422706 中川 忠彦 Nagakatsu Harada 原田 永勝 吉田 将紀 宮本 愛子 川西 由希子 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi ?橋 章 Hiroshi Sakaue 阪上 浩 Yutaka Nakaya 中屋 豊 脂質合成律速酵素グリセロール-3-リン酸アシルトランスフェラーゼ2の膜トポロジー解析 第240回徳島医学会学術集会 20100200 6339428/presentations/16422710 Tuyoshi Okahisa 岡久 強志 Yohsuke Kinouchi 木内 陽介 Masatake Akutagawa 芥川 正武 Akira Takahashi ?橋 章 Miku Maeda 前田 未来 Tomohiro Oshita 大下 知洋 Mai Katayama 片山 麻衣 X. Lian 廉 馨 Development of underwater sterilization device using UV-LED UV-LED を用いた水中殺菌装置の開発 Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20090926 6339428/presentations/16422709 Tomohiro Oshita 大下 知洋 Mai Katayama 片山 麻衣 Tuyoshi Okahisa 岡久 強志 Miku Maeda 前田 未来 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 X. Lian 廉 馨 Comparison of the disinfection using UVA-LED of the different wavelength 異なる波長のUVA-LEDを用いた殺菌作用の比較 Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20090926 6339428/presentations/16422708 Miku Maeda 前田 未来 Mutsumi Aihara 粟飯原 睦美 Xin Lian 廉 馨 Yohsuke Kinouchi 木内 陽介 Masatake Akutagawa 芥川 正武 Akira Takahashi 橋 章 New surface sterilization system using UV-LED for vegetables UV-LED を用いた野菜の表面殺菌 Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20090926 6339428/presentations/16422707 中川 亮佑 城山 和己 山中 雅斗 Kenji Teranishi 寺西 研二 Naoyuki Shimomura 下村 直行 Akira Takahashi 橋 章 バイオエレクトリクス実験のためのパルス電界印加システムの開発 平成21年度電気関係学会四国支部連合大会講演論文集 20090926 6339428/presentations/16422711 Masatake Akutagawa 芥川 正武 Miku Maeda 前田 未来 Tsuyoshi Okahisa 岡久 強志 Tomohiro Oshita 大下 知洋 Masayuki Nakano 中野 政之 Akira Takahashi ?橋 章 Masayuki Yamato 大和 正幸 Yohsuke Kinouchi 木内 陽介 Xin Lian 廉 馨 Development of sterilization devices using UV-LED UV-LEDを用いた殺菌装置の開発 The Institute of Electrical Engineers of Japan 電気学会 電気学会電子・情報・システム部門大会論文集 20090904 6339428/presentations/16422712 Qinkai Li Kazuaki Mawatari Nagakatsu Harada 中野 政之 Akira Takahashi Yutaka Nakaya Toshio Hosaka Bayasgalan Jambaldorj 大塚 良 Makoto Funaki Chronic 5-Hydroxytryptamine Treatment Induces Insulin Resistance in 3T3-L1 Adipocytes by mTOR-dependent modification of IRS-1 第238回徳島医学会学術集会 20090215 6339428/presentations/16422713 原口 さやか 下畑 隆明 Kazuaki Mawatari 馬渡 一諭 Nagakatsu Harada 原田 永勝 Akira Takahashi ?橋 章 Toshikazu Takahashi ?橋 利和 中尾 俊之 水口 潤 炭谷 晴雄 Toshio Doi 土井 俊夫 Yutaka Nakaya 中屋 豊 慢性腎不全患者における早期の飢餓状態に対する就寝前夜食の効果ー間接カロリーメーターを用いた検討 第12回日本病態栄養学会学術集会 20090110 6339428/presentations/16422715 廉 馨 前田 未来 Mutsumi Aihara Akira Takahashi 八木 教行 橘 聡子 濱本 晶子 Masayuki Yamato Masatake Akutagawa 中野 政之 Yutaka Nakaya Yohsuke Kinouchi UVA-LED装置を用いた野菜の表面殺菌 第23回生体・生理工学シンポジウム 20080930 6339428/presentations/16422714 Mosrafa Gadelmoula Xin Lian 前田 未来 Mutsumi Aihara 八木 教行 片山 麻衣 濱本 晶子 Masayuki Yamato Masatake Akutagawa Yohsuke Kinouchi Yutaka Nakaya Akira Takahashi UVA-LEDを用いた空気殺菌の試み 第23回生体・生理工学シンポジウム 20080930 6339428/presentations/16422717 八木 教行 濱本 晶子 中野 政之 Masatake Akutagawa 芥川 正武 Akira Takahashi ?橋 章 Toshitaka Ikehara 池原 敏孝 Masayuki Yamato 大和 正幸 Yohsuke Kinouchi 木内 陽介 Effect of Tank-type sterilization device using 365nm UV-LED UV-LEDを用いたタンク水の殺菌効果の検証 Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20080927 6339428/presentations/16422716 前田 未来 八木 教行 Lian Xin Yohsuke Kinouchi 木内 陽介 Masatake Akutagawa 芥川 正武 Akira Takahashi 橋 章 New surface sterilization system using UV-LED for vegetables UVA-LED を用いた野菜の表面殺菌 Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20080927 6339428/presentations/16422718 Gadelmoula Mosrafa Lian Xin Maeda Miku Aihara Mutsumi Hamamoto Akiko Masayuki Yamato Yutaka Nakaya Yohsuke Kinouchi Akira Takahashi 高出力UVA-LEDを用いた空気殺菌システムの開発 日本防菌防黴学会第35回年次大会 20080911 6339428/presentations/16422719 Mutsumi Aihara 粟飯原 睦美 廉 馨 前田 未来 橘 聡子 Masayuki Yamato 大和 正幸 中野 政之 Masatake Akutagawa 芥川 正武 Yohsuke Kinouchi 木内 陽介 Akira Takahashi 橋 章 Yutaka Nakaya 中屋 豊 高出力紫外線装置を用いた野菜表面の殺菌 日本防菌防黴学会第35回年次大会 20080811 6339428/presentations/16422558 Yagi Noriyuki Mirei Mori Hamamoto Akiko Lian Xin Nakano Masayuki Masatake Akutagawa Tachibana Satoko Akira Takahashi Toshitaka Ikehara Yohsuke Kinouchi EFFECTS OF 365NM UVA-LED ON BACTERIA The Bioelectromagnetics Society The Bioelectromagnetics Society 30th Annual Meeting 20080609 6339428/presentations/16422559 Akira Takahashi M. Nakano Kazuaki Mawatari Nagakatsu Harada Yutaka Nakaya Vibrio cholerae El tor Hemolysin Activates Cyclic AMT Dependent Cl-Secretory Pathways which Xaused Diarrhea 108th General Meeting of the American Society for Microbiology 20080600 6339428/presentations/16422720 八木 教行 Mirei Mori 森 美怜 濱本 晶子 中野 政之 Masatake Akutagawa 芥川 正武 橘 聡子 Akira Takahashi ?橋 章 Yohsuke Kinouchi 木内 陽介 Toshitaka Ikehara 池原 敏孝 Masayuki Yamato 大和 正幸 Development of a tank-type sterilization device and sterilizing effects using 365 nm UVA-LED UVA-LEDを用いたタンク型殺菌装置の開発と殺菌効果の検証 Japan Society of Medical Electronics and Biological Engineering 社団法人 日本生体医工学会 The 47th Annual Conference of japanese Society for Medical and Biological Engineering 第47回日本生体医工学会大会 20080509 6339428/presentations/16422721 原口 さやか 下畑 隆明 Kazuaki Mawatari 馬渡 一諭 Nagakatsu Harada 原田 永勝 Toshio Hosaka 保坂 利男 Akira Takahashi ?橋 章 中尾 俊之 Toshio Doi 土井 俊夫 Yutaka Nakaya 中屋 豊 間接カロリーメーターを用いた，慢性腎不全患者における早朝空腹時の代謝状態の検討 第62回日本栄養・食糧学会 20080504 6339428/presentations/16422722 Lian Xin Akira Takahashi Maeda Miku Yagi Noriyuki Tachibana Satoko Masayuki Yamato Tetsuro Koga Masatake Akutagawa Nakano Masayuki Yutaka Nakaya Yohsuke Kinouchi New surface sterilization system using UVA-LED for vegetables 第82回日本感染症学会総会・学術講演会 20080418 6339428/presentations/16422562 Yagi Noriyuki Mirei Mori Hamamoto Akiko Nakano Masayuki Masatake Akutagawa Tachibana Satoko Akira Takahashi Toshitaka Ikehara Yohsuke Kinouchi Development of a tank-type sterilization device using 365 nm UVA-LED The Society of Instrument and Control Engineers Proceedings of the International Symposium on Biological and Physiological Engineering /The 22nd SICE Symposium on Biological and Physiological Engineering 20080113 6339428/presentations/16422561 Lian Xin Akira Takahashi Maeda Miku Yagi Noriyuki Tachibana Satoko Masayuki Yamato Hamamoto Akiko Masatake Akutagawa Nakano Masayuki Yutaka Nakaya Yohsuke Kinouchi New surface sterilization system using UV-LED for vegetables The Society of Instrument and Control Engineers Proceedings of the International Symposium on Biological and Physiological Engineering /The 22nd SICE Symposium on Biological and Physiological Engineering 20080113 6339428/presentations/16422560 Hamamoto Akiko Akira Takahashi Nakano Masayuki Lian Xin Tachibana Satoko Yagi Noriyuki Tetutani Kayo Masayuki Yamato Masatake Akutagawa Toshitaka Ikehara Yutaka Nakaya Yohsuke Kinouchi Availability of UVA-light emitting diodes for disinfection system The Society of Instrument and Control Engineers 第22回生体・生理工学シンポジウム 20080113 6339428/presentations/16422564 Nakano Masayuki Akira Takahashi Yutaka Nakaya Adrenergic regulation of Vibrio parahaemolyticus pathogenicity by the modulation of viburence-associated gene expressions VIBRIO 2007(The second conference on the Biology of Vibrios) 20071128 6339428/presentations/16422563 Lian Xin Akira Takahashi Nakano Masayuki Yutaka Nakaya Vibrio parahaemolyticus elevates interferon alpha production in intestinal-like epithelial cells CACO-2 cells VIBRIO 2007(The second conference on the Biology of Vibrios) 20071128 6339428/presentations/16422723 八木 教行 Mirei Mori 森 美怜 濱本 晶子 橘 聡子 中野 政之 Masatake Akutagawa 芥川 正武 Akira Takahashi ?橋 章 Toshitaka Ikehara 池原 敏孝 Yohsuke Kinouchi 木内 陽介 Tank-type sterilization device using 365nm UV-LED UV-LEDを用いたタンク水の殺菌 Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20070929 6339428/presentations/16422724 濱本 晶子 中野 政之 八木 教行 Masatake Akutagawa 芥川 正武 橘 聡子 Toshitaka Ikehara 池原 敏孝 Akira Takahashi ?橋 章 Yohsuke Kinouchi 木内 陽介 Yutaka Nakaya 中屋 豊 高出力紫外線発光ダイオードを用いた殺菌 日本防菌防黴学会 第34回年次大会 20070830 6339428/presentations/16422565 Noriyuki Yagi Mirei Mori Akiko Hamamoto Masatake Akutagawa Satoko Tachibana Akira Takahashi Toshitaka Ikehara Yohsuke Kinouchi Masayuki Nakano Sterilization Using 365 nm UV-LED. 29th IEEE EMBS Annual International Conference 20070823 6339428/presentations/16422725 八木 教行 森 美玲 濱本 晶子 橘 聡子 中野 政之 Masatake Akutagawa 芥川 正武 Akira Takahashi ?橋 章 Toshitaka Ikehara 池原 敏孝 Yohsuke Kinouchi 木内 陽介 UV-LEDを用いたタンク水の殺菌 MEとバイオサイバネティックス研究会(MBE) 20070720 6339428/presentations/16422567 Akira Takahashi Nakano Masayuki Miyoshi Shinichi Hamamoto Akiko Yutaka Nakaya Pore formation of Vibrio mimicus hemolysin in lipid bilayers. 107th ASM general meeting 20070521 6339428/presentations/16422566 Hamamoto Akiko Mori Mirei Nakano Masayuki Wakikawa Noriko Toshitaka Ikehara Yohsuke Kinouchi Masatake Akutagawa Akira Takahashi Yutaka Nakaya Water disinfection system using UVA-light emitting diodes and its effects on bacterial DNA. 107th ASM general meeting 20070521 6339428/presentations/16422727 Akira Takahashi ? ナグビブリオが産生するエルトールヘモリシンの下痢誘発機構 第81回日本感染症学会総会 20070410 6339428/presentations/16422726 濱本 晶子 中野 政之 Akira Takahashi 橋 章 近紫外線発光ダイオードを用いた殺菌システム 第81回日本感染症学会総会 20070410 6339428/presentations/16422728 濱本 晶子 Akira Takahashi ?橋 章 Mirei Mori 森 美怜 中野 政之 Toshitaka Ikehara 池原 敏孝 Yohsuke Kinouchi 木内 陽介 高出力紫外線発光ダイオードを用いた殺菌装置の開発 Japanese Society for Bacteriology 日本細菌学会 日本細菌学雑誌「第80回日本細菌学会総会」 20070327 6339428/presentations/16422729 Mirei Mori 森 美怜 濱本 晶子 Nakano Masayuki 中野 政之 Masatake Akutagawa 芥川 正武 Akira Takahashi ?橋 章 Toshitaka Ikehara 池原 敏孝 Yohsuke Kinouchi 木内 陽介 Sterilization by UV-LED 紫外発光LEDによる殺菌 The Society of Instrument and Control Engineers 計測自動制御学会 BPES 2006 Proceedings of the 21th Symposium on Biological and Physiological Engineering 第21回生体・生理工学シンポジウム論文集 20061119 6339428/presentations/16422569 Nakano Masayuki Akira Takahashi Yutaka Nakaya Norepinephrine modulates the pathogencity of Vibrio parahaemolyticus. The 41st Joint Conference US-Japan Cooperative Medical Science Program Cholera Panel 20061100 6339428/presentations/16422568 Akira Takahashi Hamamoto Akiko Wakikawa Noriko Mori Mirei Nakano Masayuki Masatake Akutagawa Yohsuke Kinouchi Yutaka Nakaya New water sterilization system used by UVA-LED. The 41st Joint Conference US-Japan Cooperative Medical Science Program Cholera Panel 20061100 6339428/presentations/16422570 Morishima Masaki Akira Takahashi Yutaka Nakaya Transthyretin and serotonin levels in the hippocampus are decreased in SPORTS rats. 36th Society for Neuroscience 20061000 6339428/presentations/16422730 Mirei Mori 森 美怜 濱本 晶子 Nakano Masayuki 中野 政之 Masatake Akutagawa 芥川 正武 Akira Takahashi ?橋 章 Toshitaka Ikehara 池原 敏孝 Yohsuke Kinouchi 木内 陽介 紫外発光LEDの病原性菌への影響 Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20060926 6339428/presentations/16422731 Yutaka Nakaya 中屋 豊 安井 苑子 Kazuaki Mawatari 馬渡 一諭 Akira Takahashi ?橋 章 Nagakatsu Harada 原田 永勝 Hossein Nazari Takashi Kawano 河野 崇 Shuzo Oshita 大下 修造 古川 哲史 機能性食品の心血管系イオンチャネルに対する作用-Phytoestrogenのgenomicおよびnongenomic action 長井長義記念シンポジウム 20060900 6339428/presentations/16422571 Mirei Mori Akiko Hamamoto Masayuki Nakano Masatake Akutagawa Akira Takahashi Toshitaka Ikehara Yohsuke Kinouchi Effect of ultraviolet LED on bacteria Springer-Verlag Proceedings of 2006 World Congress on Medical Physics and Biomedical Engineering, Seoul, Korea, August 27-September 1, 2006 20060827 6339428/presentations/16422732 Mirei Mori 森 美怜 濱本 晶子 Nakano Masayuki 中野 政之 Masatake Akutagawa 芥川 正武 Akira Takahashi ?橋 章 Toshitaka Ikehara 池原 敏孝 Yohsuke Kinouchi 木内 陽介 紫外発光LEDを用いた殺菌 The Illuminationg Engineering Institute of Japan 社団法人 照明学会 Proceedings of 2006 Annual Conference of The llluminating Engineering Institute of Japan 平成18年度(第39回)照明学会全国大会講演論文集 20060824 6339428/presentations/16422733 Akira Takahashi ? New water sterilization system used by UVA-LED 日米コレラ部会 日本側総会 20060800 6339428/presentations/16422572 Mirei Mori Hamamoto Akiko Nakano Masayuki Masatake Akutagawa Akira Takahashi Toshitaka Ikehara Yohsuke Kinouchi Effects of ultraviolet LED on bacteria. World Congress on Medical Physics and Biomedical Engineering 2006 20060800 6339428/presentations/16422776 Mirei Mori 森 美怜 濱本 晶子 Nakano Masayuki 中野 政之 Masatake Akutagawa 芥川 正武 Akira Takahashi ?橋 章 Toshitaka Ikehara 池原 敏孝 Yohsuke Kinouchi 木内 陽介 Effects of Ultraviolet LED on E. coli 紫外発光LEDの細菌への影響 Institute of Electronics, Information and Communication Engineers 電子情報通信学会 IEICE Technical Report 電子情報通信学会技術研究報告 20060714 6339428/presentations/16422734 森島 真幸 Yutaka Nakaya 中屋 豊 Akira Takahashi ?橋 章 磯本 正二郎 小野 克重 自発的高運動性ラットSPORTSの海馬ノルエピネフリン神経伝達と血圧制御の異常． 第5回九州脳と高血圧研究会 20060701 6339428/presentations/16422575 Nakano Masayuki Akira Takahashi Sakai Yuko Yutaka Nakaya Response of Vibrio parahaemolytiocus to neuroendcrine hormone norepinephrine (D-026). 106th ASM general meeting 20060500 6339428/presentations/16422574 Akiko Hamamoto Masayuki Nakano Toshitaka Ikehara Masatake Akutagawa Yohsuke Kinouchi Yutaka Nakaya Akira Takahashi A new water sterilization system which use UVA-light emitting diode (Q-401). 106th ASM general meeting 20060500 6339428/presentations/16422573 Morishima Masaki Nagakatsu Harada Hara Sayuri Akira Takahashi Yutaka Nakaya Hippocampal norepinephrine (NE) level and exercise behavior in SPORTS rats. 15th Annual Meeting of the International Behavioral Neuroscience Society 20060500 6339428/presentations/16422576 Nakano Masayuki Akira Takahashi Sakai Yuko Yutaka Nakaya Norepinephrine modulates the pathogenicity of Vibrio parahameolyticus. 11th Asian conference on diarrhoeal diseases and nutrition 20060300 6339428/presentations/16422735 Mirei Mori 森 美怜 Nakano Masayuki 中野 政之 Masatake Akutagawa 芥川 正武 Akira Takahashi ?橋 章 Toshitaka Ikehara 池原 敏孝 Yohsuke Kinouchi 木内 陽介 Studies of sterilized effects of ultraviolet LED 紫外発光LEDを用いた殺菌作用に関する研究 Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers 電気関係学会四国支部連合大会講演論文集 20050928 6339428/presentations/16422577 Nakano Masayuki Akira Takahashi Sakai Yuko Yutaka Nakaya Response of enteropathogen to neuroendocrine hormone . 2005 COE international conference on biological mechanism for stress control COE international conference on biological mechanism for stress control 20050817 6339428/presentations/16422777 Mirei Mori 森 美怜 Nakano Masayuki 中野 政之 Masatake Akutagawa 芥川 正武 Akira Takahashi ?橋 章 Toshitaka Ikehara 池原 敏孝 Yohsuke Kinouchi 木内 陽介 紫外発光LEDの大腸菌への影響 Institute of Electronics, Information and Communication Engineers 電子情報通信学会 IEICE Technical Report 電子情報通信学会技術研究報告 20050730 6339428/presentations/16422578 Akira Takahashi Nakano Masayuki Miyoshi Shinichi Nagakatsu Harada Yutaka Nakaya (B-053) Hemolysin produced by Vibrio mimicus activates two Cl- secretory pathways in cultured intestinal-like cells. 105th American society for microbiology general meeting 105th American society for microbiology general meeting 20050605 6339428/presentations/16422736 森島 真幸 原 小由合 Nagakatsu Harada 原田 永勝 Atsuko Sano 佐野 敦子 Hiromasa Seno 妹尾 廣正 Akira Takahashi 橋 章 Yutaka Nakaya 中屋 豊 高運動性モデルラットSPORTSにおける海馬ノルエピネフリン動態は運動習性を調節する. 第82回日本生理学会 20050520 6339428/presentations/16422737 森島 真幸 Nagakatsu Harada 原田 永勝 原 小由合 Atsuko Sano 佐野 敦子 Hiromasa Seno 妹尾 廣正 Akira Takahashi 橋 章 Yutaka Nakaya 中屋 豊 高運動性モデルラットSPORTSにおける運動習性と脳内モノアミン制御. 第59回日本栄養食糧学会 20050515 6339428/presentations/16422738 中野 政之 児玉 年央 Akira Takahashi 橋 章 ノルエピネフリンによる腸炎ビブリオの病原性に対する影響 第78回日本細菌学会総会 20050404 6339428/presentations/16422739 Akira Takahashi ? ?V Yutaka Nakaya ? ?L 腸管内細菌感染による腸管上皮細胞のクロライド分泌変化 第1回日本消化管学会 20050117 6339428/presentations/16422740 Takuya Seike 清家 卓也 Hideki Nakanishi 中西 秀樹 Kazuya Matsumoto 松本 和也 Shingo Kawakami 川上 慎吾 Keiji Moriyama 森山 啓司 Akira Takahashi 橋 章 顎裂骨移植の評価と予後予測-裂の形態と骨塩量について- 第45回日本形成外科学会総会・学術集会 20020417 6339428/presentations/16422741 Mari Miki 三木 真理 Youichi Nakamura 中村 陽一 Hiroyuki Hino 日野 弘之 Fumitaka Okushi 大串 文隆 Akira Takahashi ?橋 章 Sumiko Yoshinaga 吉永 純子 Hiroshi Maezawa 前澤 博 Susumu Yasuoka 安岡 劭 ヒト気道トリプシン様プロテアーゼ(HAT)の気道細胞内遊離Ca2+濃度[Ca2+]に対する作用 The Japanese Respiratory Society 社団法人 日本呼吸器学会 第42回日本呼吸器学会総会プログラム 20020405 6339428/presentations/16422742 Keiko Hosokawa 細川 敬子 Hisao Yamaguchi 山口 久雄 Toshitaka Ikehara 池原 敏孝 Akira Takahashi 橋 章 Kazuo Yoshizaki 吉? 和男 低K+条件下での細胞周期調節因子の発現量とその局在 The Physiological Society of Japan 日本生理学会 第74回日本生理学会大会 19970326 6339428/presentations/16422743 Toshitaka Ikehara 池原 敏孝 Hisao Yamaguchi 山口 久雄 Keiko Hosokawa 細川 敬子 Akira Takahashi ?橋 章 Hiroshi Miyamoto 宮本 博司 Kazuo Yoshizaki 吉? 和男 HeLa細胞の利尿剤感受性K+排出とRb+取込みに及ぼす細胞内外イオンの影響 The Physiological Society of Japan 日本生理学会 第73回日本生理学会大会 19960405 6339428/presentations/16422744 Keiko Hosokawa 細川 敬子 Hisao Yamaguchi 山口 久雄 Toshitaka Ikehara 池原 敏孝 Akira Takahashi ?橋 章 Kazuo Yoshizaki 吉? 和男 Hiroshi Miyamoto 宮本 博司 同調した細胞の細胞周期調節蛋白の発現とKイオンの役割 The Physiological Society of Japan 日本生理学会 第73回日本生理学会大会 19960403 6339428/presentations/16422746 Toshitaka Ikehara 池原 敏孝 Hisao Yamaguchi 山口 久雄 Keiko Hosokawa 細川 敬子 Akira Takahashi ?橋 章 Kazuo Yoshizaki 吉? 和男 Hiroshi Miyamoto 宮本 博司 培養HeLa細胞における利尿剤感受性外向きと内向きK+輸送 The Physiological Society of Japan 日本生理学会 第72回日本生理学会大会 19950331 6339428/presentations/16422745 Keiko Hosokawa 細川 敬子 Hisao Yamaguchi 山口 久雄 Toshitaka Ikehara 池原 敏孝 Akira Takahashi 橋 章 Kazuo Yoshizaki 吉? 和男 低K+条件下におけるHeLa細胞の細胞周期調節因子の発現 The Physiological Society of Japan 日本生理学会 第72回日本生理学会大会 19950331