Secretion dynamics of cytotoxic granules in the cytotoxicity of the human NK-like cultured cells KHYG-1

ヒトNK様培養細胞KHYG‐1の細胞傷害における細胞傷害性顆粒の分泌動態


Evaluation of NK cell function using human NK-like cultured cell, KHYG-1

ヒトNK様培養細胞KHYG-1を用いたNK細胞機能の評価

NK cells play an important role in the elimination of viral infection and tumors. In recent years, immune cell therapy using activated NK cells has attracted attention, and the search for activators and evaluation of NK cell activity have become important. We previously reported a positive correlation between CD56antigen expression, which is the major cell membrane antigen of human NK cells, and NK cell activity or cytotoxicity, and demonstrated that it is possible to evaluate NK cell function using the CD56 antigen as an index. This simple evaluation method is useful for functional evaluation of NK cells and the search for activators. However, it requires blood sampling and preparation of NK cells because it uses human NK cells. Therefore, in this study, we examined whether the CD56 antigen functions as an activation index using KHYG‐1 human NK-like cultured cells as a substitute for NK cells. As a result, the CD56antigen on the KHYG‐1cell membrane was increased in a concentration-dependent manner by IL‐2 stimulation, as was the cytotoxicity. This suggests that the CD56 antigen on the KHYG‐1 cell membrane can be used as an evaluation index of NK activity as in human NK cells.


ABO genotyping of various hematopoietic cell lines to select model cells for research purposes


Evaluation of effects of various drugs on platelet functions using phorbol 12-myristate 13-acetate-induced megakaryocytic human erythroid leukemia cells

The hyperfunction and activation of platelets have been strongly implicated in the development and recurrence of arterial occlusive disease, and various antiplatelet drugs are used to treat and prevent such diseases. New antiplatelet drugs and many other drugs have been developed, but some drugs may have adverse effects on platelet functions. The aim of this study was to establish an evaluation method for evaluating the effect and adverse effect of various drugs on platelet functions. Human erythroid leukemia (HEL) cells were used after megakaryocytic differentiation with phorbol 12-myristate 13-acetate as an alternative to platelets. Drugs were evaluated by changes in intracellular Ca(2+) concentration ([Ca(2+)]i) mobilization in Fura2-loaded phorbol 12-myristate 13-acetate-induced HEL cells. Aspirin and cilostazol were selected as antiplatelet drugs and ibuprofen and sodium valproate as other drugs. There was a positive correlation between [Ca(2+)]i and platelet aggregation induced by thrombin. Aspirin (5.6-560 µM) and cilostazol (5-10 µM) significantly inhibited thrombin-induced increases in [Ca(2+)]i in a concentration-dependent manner. On the other hand, ibuprofen (8-200 µM) and sodium valproate (50-1,000 µg/mL) also significantly inhibited thrombin-induced increases in [Ca(2+)]i in a concentration-dependent manner. Furthermore, the interaction effects of the simultaneous combined use of aspirin and ibuprofen or sodium valproate were evaluated. When the inhibitory effect of aspirin was higher than that of ibuprofen, the effect of aspirin was reduced, whereas when the inhibitory effect of aspirin was lower than that of ibuprofen, the effect of ibuprofen was reduced. The combination of aspirin and sodium valproate synergistically inhibited thrombin-induced [Ca(2+)]i. It is possible to induce HEL cells to differentiate into megakaryocytes, which are a useful model for the study of platelet functions, and the quantification of the inhibition of thrombin-induced increases in [Ca(2+)]i is applicable to the evaluation of the effects of various drugs on platelets.


Flow Cytometric Evaluation of Surface CD56 Expression on Activated Natural Killer Cells as Functional Marker.

Surface CD56 is the most important cell marker for defining NK cells. However, the relationship between the expression of surface CD56 and NK cell activity has not yet been elucidated in detail. Thirteen healthy volunteers were enrolled in the present study. Peripheral blood mononuclear cells (PBMCs) were stimulated with rIL-2 or rIL-12 (1, 10, 100 U/mL) for 18 h at 37°C. After incubation, surface CD56 expression on NK cells was evaluated using a flow cytometric analysis. A colorimetric-based lactate dehydrogenase (LDH) assay was used for experiments on cytotoxicity. IFN- mRNA gene expression was quantified by real-time PCR. The expression level of surface CD56 on NK cells, cytotoxicity, and IFN- mRNA gene expression were significantly increased by the rIL-2 and rIL-12 stimulations. In addition, a positive correlation was found between surface CD56 expression and cytotoxic activity or IFN- mRNA gene expression. We revealed that the quantification of surface CD56 expression was applicable to the evaluation of cytotoxicity and IFN- production in activated NK cells. These results suggest that the measurement of surface CD56 expression represent an easy and rapidly reproducible technique to evaluate the activated state of NK cells and monitor NK cell activity in immunotherapy. J. Med. Invest. 63: 199-203, August, 2016.

The Journal of Medical Investigation : JMI


SNPs rs4656317 and rs12071048 located within an enhancer in FCGR3A are in strong linkage disequilibrium with rs396991 and influence NK cell-mediated ADCC by transcriptional regulation.

CD16 receptors are mainly expresses on the surface of NK cells and mediate antibody-dependent cellular cytotoxicity (ADCC). The authors previously reported that NK cell-mediated ADCC is influenced by the single nucleotide polymorphism (SNP) rs396991 (T>G; F158V), and the structure and expression levels of CD16 differed among these genotypes. The authors examined haplotype frequency distributions among rs396991 and other SNPs, rs10917571 (G>T), rs4656317 (C>G), and rs12071048 (G>A), located in an enhancer of the FCGR3A gene. A total of 101 healthy Japanese were genotyped for the presence of these SNPs. The authors also measured ADCC activity, FCGR3A transcript levels, and surface CD16 expression on NK cells. We found that the regulatory SNPs (rSNPs) rs4656317 and rs12071048 were in strong linkage disequilibrium with rs396991. These two SNPs with major alleles had higher ADCC activity than those with minor alleles. In addition, FCGR3A transcript levels and surface CD16 expression levels were regulated by these SNPs. These findings suggest that NK cell-mediated ADCC could be influenced by transcriptional regulation of these rSNPs. These findings help to clarify our understanding of the linkage disequilibrium among functional SNPs in the FCGR3A gene, and provide a resource for investigating the roles of functional SNPs in NK cell-mediated ADCC.


The influence of NK cell-mediated ADCC: Structure and expression of the CD16 molecule differ among FcRIIIa-V158F genotypes in healthy Japanese subjects

NK cells express the CD16 (FcRIIIa) receptor, which mediates antibody-dependent cellular cytotoxicity (ADCC), on their cell surface. Therefore, ADCC activity may be influenced by qualitative or quantitative changes in the CD16 molecule on NK cells. Responses to NK cell-mediated ADCC have been shown to depend on single nucleotide polymorphisms (SNPs) at FcRIIIa amino acid position 158. However, a consensus has not yet been reached regarding differences in the structure and expression levels of the CD16 molecule among FcRIIIa-V158F genotypes, which have not yet been adequately investigated in healthy Japanese individuals. We herein examined the influence of the FcRIIIa polymorphism on ADCC, binding affinity of CD16 to the Fc region, FCGR3A gene expression, and cell-surface CD16 expression in healthy Japanese subjects. FcRIIIa-V158F genotyping was performed for 101 subjects. The results obtained showed that all parameters analyzed increased in the order of V/V>V/F>F/F and were significantly higher in V/V subjects than in F/F subjects. Moreover, a positive correlation was observed between ADCC activity and binding affinity, FCGR3A transcript levels, and surface CD16 expression levels. These results suggest that the structure and expression of the CD16 molecule differs among FcRIIIa-V158F genotypes, and the FcRIIIa-V158F polymorphism may be represent a haplotype with other SNPs in regulatory regions in Japanese subjects.


Direct Determination of ABO Blood Group Genotypes from Whole Blood Using PCR-Amplification of Specific Alleles Method


Effects of lavender and grapefruit essential oil on the autonomic nervous system and immune system

ラベンダー精油とグレープフルーツ精油による自律神経系および免疫系に及ぼす影響


The evaluation of histo-blood group ABO typing by flow cytometric and PCR Amplification of Specific Alleles analyses and their application in clinical laboratories

ABO antigens are oligosaccharide antigens, and are widely distributed on red blood and tissue cells as well as in saliva and body fluid. Therefore, these antigens are important not only for blood transfusion, but also for tissue cell and organ transplantations. Also, blood, hair, and seminal fluid are important sources of evidence at crime scenes, and these antigens are some of the most important markers for personal identification in forensic investigations. Here, we describe the development and use of quantitative analysis of A, B, and H antigens on red blood cells by employing flow cytometric analysis and the ABO genotyping method based on PCR-amplification of specific alleles (PASA) within DNA, especially from blood and saliva. In this study, flow cytometric analysis could be used to compare the differences between the expression of A and/or B and H antigens on red blood cells with various phenotypes, and the PASA method was able to determine the genotype of the type cisA(2)B(3) pedigree using only DNA extracted from saliva. These analysis methods are simple and useful for judging the ABO blood group system and genotyping, and are used widely throughout research and clinical laboratories and forensic fields.


Preparation method for a specific anti-H lectin isolated from goya seeds and its application in clinical laboratories

ゴーヤ種子抽出抗Hレクチンの臨床検査への応用

After the discovery of the phenomenon of aggregation of red blood cells with castor beanextract by Peter Hermann Stillmark in1888, various plant hemagglutinins were discovered fromleguminous plant seed extracts. These plant hemagglutinins were called"lectins"by William C.Boyd et al . Lectins are sugar-binding proteins that are highly specific for their sugar moieties,physiological activity, and hemagglutinating activity. In particular, lectins displaying blood-groupspecificity are important for blood-group typing and antigen recognition. In recent years, Ulexlectin(anti-H lectin)extracted from the Ulex europaeus seed was used to subdivide blood of typeO, and the subgroup of ABO, but Ulex europaeus is now difficult to obtain, so it is necessary to buyexpensive reagents.On the other hand, Momordica charantias (goya)seed extract(goya lectin)was recentlyrevealed to show higher anti-H activity than Ulex lectin. Goya is readily available and inexpensiveto obtain. Therefore, we examined a simple method for the preparation of goya lectin.As a result, we were able to establish a simple method for preparing goya lectin isolated fromgoya seeds, and confirm its stability against long-term storage and temperature and usefulness asan anti-H lectin. We expect this method to be used in clinical practice in the future.


全国20国立大学の保健学科検査科学専攻分野のカリキュラム・卒業後進路

全国20国立大学の保健学科検査科学専攻分野のカリキュラム・卒業後進路

Japanese journal of medical technology education


Differential alterations in expressions of ryanodine receptor subtypes in cerebellar cortical neurons of an ataxic mutant, rolling mouse Nagoya

This study aimed to clarify changes in the spatial expressions of types 1, 2 and 3 ryanodine receptors (RyR1, RyR2 and RyR3) in the cerebellum of a Ca(2+) channel alpha(1A) subunit mutant, rolling mouse Nagoya. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) revealed that the mRNA signal levels of RyR1 and RyR3 were altered in the rolling cerebellum, which exhibited lower densities of RyR1 bands and higher densities of RyR3 bands than in the control cerebellum. Quite consistent with the RT-PCR results, the staining intensity of RyR1 and RyR3 was altered in the rolling cerebellum. RyR1 immunostaining appeared in somata and the proximal dendrites of Purkinje cells, and the staining intensity of both subcellular regions was equally lower in all cerebellar lobules of rolling mice than in those of controls. Although RyR3 immunostaining appeared in the dendrites of granule cells, more intense RyR3 staining in rolling mice than in controls was uniformly observed throughout all cerebellar lobules. The present study further examined co-localizations of ryanodine receptor subtypes and voltage-gated Ca(2+) channel alpha(1) subunits in the rolling cerebellum. Somatodendritic RyR1 immunostaining in Purkinje cells overlapped with either a mutated Ca(2+) channel alpha(1A) subunit (P/Q-type), or a Ca(2+) channel alpha(1C) subunit (L-type; dihydropyridine receptor) immunostaining. Immunostaining of these alpha(1) subunits also emerged in granule cells. Those results suggest non-region-related alterations in RyR1 and RyR3 expressions in the rolling mouse cerebellum. Such expressional changes in ryanodine receptor subtypes may be involved in Ca(2+) channel alpha(1A) subunit gene mutation, and may alter regulation of intracellular Ca(2+) concentrations in cerebellar cortical neurons.


シクロオキシゲナーゼII阻害薬のヒト血小板凝集に及ぼす影響

シクロオキシゲナーゼII阻害薬のヒト血小板凝集に及ぼす影響


Expression levels of H-type alpha (1,2)-fucosyl-transferase gene and histo-blood group ABO gene corresponding to hematopoietic cell differentiation

The expression of ABO antigens on the surface of RBCs is regulated by ABO gene-encoded ABO transferase activity after the formation of the H antigen. The molecular mechanisms that control the expression of the ABO blood group antigens along with erythroid differentiation are one of the most important subjects of study in transfusion science. FUT1(H), ABO, and beta 2-microglobulin mRNA were determined by semiquantitative RT-PCR from 27 hematopoietic cultured cell lines showing various differentiation stages and cell lineages and normal PBMNCs. The expression level of each cell was analyzed with computer software (Image, NIH) and was shown as the ratio of ABO mRNA or H mRNA to beta 2-microglobulin mRNA. The H antigen was also determined by immunocytochemical methods with flow cytometry in some cell lines. The highest expression of H mRNA was found in KOPM-28 and HEL cell lines and a lower expression was found in the mature cells. In contrast, it was observed that H antigen expression began at the level of HEL and PL-21 cells and increased with cell maturation. The highest expression of ABO mRNA was found in K-562 and KOPM-28 cell lines and it decreased along with cell maturation. Based on these results, it is concluded that the transcription of both H and ABO genes starts early in immature peripheral blood progenitor cells but gradually decreases during cellular maturation and also that the H antigen maintains a high level of expression thereafter. These results may reflect the active synthesis of H and ABO antigens in normal hematopoietic peripheral blood progenitor cells.


Patients with malignant hyperthermia demonstrate an altered calcium control mechanism in B lymphocytes

Altered Ca2+ homeostasis in skeletal muscle is a key molecular event triggering malignant hyperthermia (MH) in malignant hyperthermia-susceptible (MHS) individuals. Genetic studies have shown that mutations in the type 1 ryanodine receptor (RYR1) are associated with MH susceptibility. Because human B lymphocytes express the RYR1, it is hypothesized that Ca2+ homeostasis in B lymphocytes is altered in MHS individuals. This study investigated the Ca2+ response of B cells to caffeine and 4-chloro-m-cresol in 13 MHS and 21 MH-negative (MHN) individuals who had been diagnosed by caffeine halothane contracture test (CHCT) and 18 healthy volunteers. Changes in [Ca2+]i in B cells were measured directly in fluo-3 loaded cells using a dual-color flow cytometric technique. Further, B cell phenotype was correlated with CHCT results in a family with the Val2168Met (G6502A) mutation. Caffeine-induced (50 mm) increases in [Ca2+]i in B cells were significantly greater in MHS than in MHN (P = 0.0004), control (P = 0.0001) or non-MHS (MHN and control) individuals (P < 0.0001). The 4-chloro-m-cresol-induced (400 microm) increases in [Ca2+]i were also significantly different between MHS and controls (P = 0.003) or between MHS and non-MHS (MHN and control) individuals (P = 0.0078). A study of a family with the Val2168Met mutation demonstrated expression of the RYR1 mRNA mutant in B cells from the family members with MHS phenotype and a clear segregation of genotype with B-cell phenotype. The Ca2+ responses to caffeine or 4-chloro-m-cresol in B lymphocytes showed significant differences between MHS and MHN (or control) individuals. Although the molecular mechanisms of these alterations are currently undetermined, the results suggest that the enhanced Ca2+ responses are associated with mutations in the RYR1 gene in some MHS individuals.


Expression of the ryanodine receptor isoforms in immune cells

Ryanodine receptor (RYR) is a Ca(2+) channel that mediates Ca(2+) release from intracellular stores. We have used RT-PCR analysis and examined its expression in primary peripheral mononuclear cells (PBMCs) and in 164 hemopoietic cell lines. In PBMCs, type 1 RYR (RYR1) was expressed in CD19(+) B lymphocytes, but less frequently in CD3(+) T lymphocytes and in CD14(+) monocytes. Type 2 RYR (RYR2) was mainly detected in CD3(+) T cells. Induction of RYR1 and/or RYR2 mRNA was found after treatment with stromal cell-derived factor 1, macrophage-inflammatory protein-1alpha (MIP1alpha) or TGF-beta. Type 3 RYR (RYR3) was not detected in PBMCs. Many hemopoietic cell lines expressed not only RYR1 or RYR2 but also RYR3. The expression of the isoforms was not associated with specific cell lineage. We showed that the RYR-stimulating agent 4-chloro-m-cresol (4CmC) induced Ca(2+) release and thereby confirmed functional expression of the RYR in the cell lines expressing RYR mRNA. Moreover, concordant induction of RYR mRNA with Ca(2+) channel function was found in Jurkat T cells. In untreated Jurkat T cells, 4CmC (>1 mM) had no effect on Ca(2+) release, whereas 4CmC (<400 microM) caused Ca(2+) release after the induction of RYR2 and RYR3 that occurred after treatment with stromal cell-derived factor 1, macrophage-inflammatory protein-1alpha, or TGF-beta. Our results demonstrate expression of all three isoforms of RYR mRNA in hemopoietic cells. Induction of RYRs in response to chemokines and TGF-beta suggests roles in regulating Ca(2+)-mediated cellular responses during the immune response.


Effect of MDR antagonists on the cidal activity of vincristine for cells expressing MDR-1 is superior to those expressing MRP


Overcoming of cross-resistance to the cell-killing activityof oxygen radicals in VCR-resistant hematologic cell lines with cyclosporin-A


Detection of histo-blood groop ABOmRNA in human chronic myeroid leukemia cell lines using reverse transcription-polymerase chain reaction(RT-PCR)


Secretory profile of immunoreactive growth hormone-releasing hormone (IR-GHRH) during sleep in man and its clinical value

To clarify the role of growth hormone-releasing hormone (GHRH) in the regulation of the episodic growth hormone (GH) secretion which is known to occur constantly in the initial slow wave stage of nocturnal sleep in man, we studied the relation between the secretions of plasma immunoreactive(IR)-GHRH and GH while recording electroencephalograms. In subjects who showed a normal sleep pattern, the plasma IR-GHRH level increased 3- to 4-fold just before the surge of plasma GH, suggesting that GH release in the initial slow wave stage of sleep is mainly mediated by GHRH. However, when there was an apparent GH surge just before the onset of sleep, the magnitude of the GH response associated with the initial slow wave stage tended to be blunted, even when sufficient IR-GHRH was released. We also observed no appreciable fluctuations of plasma IR-GHRH during nocturnal sleep in a patient diagnosed as having GH-deficient dwarfism, suggesting the primary lesion was on the hypothalamus level, not the pituitary, in such a patient. In a case of multiple endocrine neoplasia (MEN) type I with an ectopic (GHRH-producing pancreatic tumor, no remarkable elevation of plasma IR-GHRH was detected in the initial slow wave stage of nocturnal sleep. We conclude that the present study is significant not only in demonstrating the physiology of GHRH release, but also in establishing a safe, reliable and practical test for routine clinical use to investigate intrinsic ability to release GHRH and the primary lesions in patients with disorders of GH secretion.


Correlation between permeability-related glycoprotein expression and susceptibility to oxygen radicals in vincristine-resistant hematologic cell lines


Immunoreactive growth hormone-releasing hormone(IR-GHRH) in the feto-placental circulation and differential effects of L-Dopa, L-Arginine and Somatostatin-14 on the plasma levels of IR-GHRH in normal adults

The relation of the physiological releases of growth hormone-releasing hormone (GHRH) and growth hormone (GH) into the circulation in various conditions was investigated using a sensitive and specific radioimmunoassay for plasma GHRH. The mean fasting plasma level of immunoreactive (IR)-GHRH in 72 normal adults was 10.3 +/- 0.5 (mean +/- SEM) pg/ml and there was no significant sex difference in the level. The concentrations of IR-GHRH in plasma from the umbilical artery and umbilical vein were 107.3 +/- 20.5 pg/ml and 33.6 +/- 3.8 pg/ml, respectively, and a marked arterio-venous gradient was observed in all 12 individuals examined. The plasma level of IR-GHRH in the maternal vein was significantly lower than that in the cord blood, but was similar to that in non-pregnant women. In normal adults, although there was no apparent fluctuation in the level of plasma IR-GHRH or of plasma GH during bed rest, a significant increase of plasma IR-GHRH was detected followed by, or synchronized with the surge of plasma GH after oral administration of L-dopa. In contrast, on L-arginine infusion, no proportional elevation of plasma IR-GHRH with increase in plasma GH was observed. During and after intravenous infusion of somatostatin, the circulating IR-GHRH level did not increase, but on stopping the infusion there was an immediate and marked rebound surge of GH. We conclude that 1) the elevated IR-GHRH in the cord blood plasma originates from the fetus and may have a primary role in enhancing secretion of GH which promotes growth in early human life, and 2) the participations of GHRH in the mechanisms of GH secretion seen after administrations of L-dopa, L-arginine and somatostatin are different.


ABO式血液型の遺伝子解析と臨床検査への応用

ABO式血液型の遺伝子解析と臨床検査への応用


アリル特異的増幅法(PASA法)を用いたABO式血液型およびcisAB型genotypeの遺伝子解析


PCR法を用いたパラフィン切片組織からのABO遺伝子増幅法

PCR法を用いたパラフィン切片組織からのABO遺伝子増幅法


毛髪DNAを用いたABO式血液型genotypeの遺伝子解析

毛髪DNAを用いたABO式血液型genotypeの遺伝子解析


健常者の右側胸部誘導心電図所見に関する検討

健常者の右側胸部誘導心電図所見に関する検討


Analysis of the Gsα gene in a growth hormone-secreting pituitary adenomas by the polymerase chain reaction-direct sequencing method using paraffin-embedded tissues


Demonstration of biological activity of a growth hormone-releasing hormone-like substance produced by a pheochromocytoma


Gsα遺伝子の点突然変異を認めたMENI 型の下垂体腺腫症例

Gsα遺伝子の点突然変異を認めたMENI 型の下垂体腺腫症例

ホルモンと臨床, 冬増刊号(間脳下垂体腫瘍 Ⅱ)

ホルモンと臨床, 冬増刊号(間脳下垂体腫瘍 Ⅱ)


ホルター心電図による心房細動例の心電図的検討

ホルター心電図による心房細動例の心電図的検討

徳島県臨床衛生検査技師会誌

徳島県臨床衛生検査技師会誌


ABO式血液型のAおよびB抗原の免疫細胞化学的解析

ABO式血液型のAおよびB抗原の免疫細胞化学的解析


A pituitary specific point mutation of codon 201 of the Gsα gene in a pituitary adenoma of a patient with multiple endocrine neoplasia (MEN) type 1


健常者の肺気量変動に伴う心電図変化に関する検討

健常者の肺気量変動に伴う心電図変化に関する検討

徳島県臨床衛生検査技師会誌

徳島県臨床衛生検査技師会誌


血液型A1型で抗B抗体の欠損した1例について

血液型A1型で抗B抗体の欠損した1例について


徳島県臨床衛生検査技師会誌

徳島県臨床衛生検査技師会誌


Case report: Subclinical pseudohypoparathyroidism type II: Evidence for failure of physiologic adjustment in calcium metabolism during pregnancy


ラットにおけるPGE2, 2-DGおよびL-arginineの視床下部GHRHおよびSRIFの放出に及ぼす影響とGH分泌との相関


ゼラチン粒子凝集法(PA法)による抗 HTLV-I抗体測定の検討

ゼラチン粒子凝集法(PA法)による抗 HTLV-I抗体測定の検討

徳島県臨床衛生検査技師会誌

徳島県臨床衛生検査技師会誌


静注用グロブリン製剤に含まれる抗赤血球抗体，抗HB抗体，抗HTLV-Ⅰ抗体およびHIV抗体の検討

The Japanese journal of medical technology


Influence of anesthetic agents on the release of growth hormone induced by growth hormone-releasing hormone in rats

The Tokushima Journal of Experimental Medicine


Secretion of growth hormone-releasing hormone in patients with idiopathic pituitary dwarfism and acromegaly


Ectopic Growth Hormone-Releasing Hormone (GHRH) Syndrome in a Case with Multiple Endocrine Neoplasia Type 1.


Effect of electric stimulation of the celiac vagus on gastric acid secretion and plasma concentrations of somatostatin and gastrin in the portal and gastroepiploic veins of dogs


ABO式血液型不適合妊娠の予測に関する血清学的検査法の検討

ABO式血液型不適合妊娠の予測に関する血清学的検査法の検討


LHRHアゴニスト(Buserelin)の正常成人男子におけるゴナドトロピンおよびテストステロンの分泌に及ぼす影響


手術時の血液使用状況ならびにMaximum Surgical Blood Order ScheduleとType & Screen の導入について

徳島県臨床衛生検査技師会誌

徳島県臨床衛生検査技師会誌


Production and secretion of immunoreactive growth hormone-releasing factor by pheochromocytomas


Immunoreactive Somatostatin and Calcitonin in Pulmonary Neuroendocrine Tumor

A well-differentiated neuroendocrine carcinoma of the lung that secreted immunoreactive somatostatin (IR-SRIF) and IR-calcitonin (CT) in a 72-year-old women is described. The plasma concentrations of IR-SRIF (57.5 pg/ml) and IR-CT (340 pg/ml) before operation were significantly higher than the respective normal ranges. After resection of the tumor, the plasma CT level (105 pg/ml) decreased to within the normal range, and the SRIF level (32.7 pg/ml) also decreased, but was still abnormally higher, which suggested the presence of an unidentified remnant of the tumor. Abnormal accumulation of technetium 99m (99mTc) in the lumbar vertebrae was found 6 months after the operation, which indicated a metastatic tumor. The tissue concentrations of IR-SRIF and IR-CT were 103 and 94 ng/g wet weight, respectively, and SRIF-IR tumor cells and CT-IR tumor cells were demonstrated immunohistochemically. On gel-filtration chromatography of the tumor tissue, two peaks of SRIF immunoreactivity were eluted in the positions of synthetic SRIF-28 and SRIF-14, respectively. Conversion of SRIF-28 to SRIF-14 was suggested from results on changes in the two IR-SRIF components during incubation with a crude enzyme preparation extracted from the tumor tissue.


徳島大学医学部附属病院輸血部における輸血業務の統計学的考察

徳島大学医学部附属病院輸血部における輸血業務の統計学的考察


輸血検査で見い出された興味ある4症例について

輸血検査で見い出された興味ある4症例について


最近の5年間における献血者の輸血検査異常値出現率について

最近の5年間における献血者の輸血検査異常値出現率について


血球洗浄システム(SIM WASH)による抗グロブリン試験の検討

血球洗浄システム(SIM WASH)による抗グロブリン試験の検討

徳島県臨床衛生検査技師会誌

徳島県臨床衛生検査技師会誌


濃縮第Ⅷ因子製剤の大量投与により溶血性貧血の発症を見た血友病Aの一症例について

濃縮第Ⅷ因子製剤の大量投与により溶血性貧血の発症を見た血友病Aの一症例について


Inhibitory action of somatostatin on meiotic maturation of cultured porcine follicular ova


Radioimmunoassay of an analog of luteinizing hormone-releasing hormone, [D-Ser(tBu)]6des-Gly-NH102 ethylamide (Busererin)

A sensitive and specific radioimmunoassay is described for plasma and urinary levels of [D-Ser(tBu)]6des-Gly-NH2(10) ethylamide (buserelin). No appreciable cross-reaction (less than 0.05%) was observed with LH-RH and its analogs other than buserelin fragments (1.6-45%). The sensitivity was 3 pg per tube. At buserelin concentrations of 125, 250 and 500 pg/ml, the intra- and inter-assay coefficients of variation were 7.9, 10.0 and 10.0%, and 19.0, 7.8 and 6.8% respectively. Recovery of buserelin added to plasma was quantitative (62.5 pg/ml, 101.6%; 125 pg/ml, 76.8% and 250 pg/ml, 63.4%). A dose of 5 micrograms buserelin injected subcutaneously into 5 normal male adults, reached a peak plasma level in 45 min (mean value 119.3 +/- 47.3 pg/ml) and remained detectable for at least 4 h. The half disappearance time was 118.8 +/- 26.0 min. Between 9 and 16% of the administered dose was excreted in the urine within 24 h. Buserelin could also be detected in the plasma after intranasal administration of doses of 150, 300 and 450 micrograms. There was a significant difference in the area under the curve (AUC) for plasma levels after subcutaneous injection of 5 micrograms and intranasal administration of 150 micrograms, but not between the AUC values after the three intranasal doses. These results indicate that this method for radioimmunoassay of buserelin is suitable for analyzing the pharmacokinetics and bioavailability of buserelin in man.


Production of growth hormone-releasing factor in pheochromocytoma


Co-storage and co-secretion of somatostatin and catecholamine in bovine adrenal medulla


Clinical value of radioimmunoassay of plasma growth-hormone-releasing factor.


Evidence for existence of somatostatin-like immunoreactivity with molecular heterogeneity in porcine ovaries


The Japanese journal of medical technology


徳島県における血液型分布に関する調査

徳島県における血液型分布に関する調査

徳島県臨床衛生検査技師会誌

徳島県臨床衛生検査技師会誌


Distribution and characteristics of immunoreactive somatostatin, vasoactive intestinal polypeptide and calcitonin in tumors


Fetal and maternal plasma levels of immunoreactive somatostatin at delivery: evidence for its increase in the umbilical artery and its arterio-venous gradient in the feto-placental circulation

The concentrations of immunoreactive somatostatin (IR-SRIF) in plasma samples taken from the umbilical artery (UA), umbilical vein (UV), and maternal antecubital vein (MV) were measured in 23 cases of normal delivery at term. High concentrations of IR-SRIF were detected in the plasma from the UA (mean +/- SD, 73.2 +/- 40.6 pg/ml), the value being about 2.5 times that in the UV (29.5 +/- 17.5 pg/ml; P less than 0.001). An arterio-venous gradient was observed in all 23 subjects. The plasma level of IR-SRIF in the MV (10.9 +/- 4.6 pg/ml) was significantly lower than those in the UA (P less than 0.001) and UV (P less than 0.001) and was almost the same as that in nonpregnant women (12.7 +/- 5.4 pg/ml). Chromatographic analysis of an extract of plasma from the UA gave only one peak of immunoreactive material, which was eluted in the same position as synthetic SRIF-14. A similar result was obtained in nonpregnant women. No correlation was found between the plasma level of IR-SRIF and that of GH, insulin, glucagon, or gastrin in the UA. The present findings suggest that the high concentration of IR-SRIF in the feto-placental circulation originates from the fetus and reflects a particular role of this peptide in the process of functional maturation of the neuroendocrine system in early human life.

The Journal of Clinical Endocrinology and Metabolism


第 4回日本臨床検査学教育学会学術大会イブニングセミナー「 6．免疫血清・輸血検査学」


Recovery of chemosensitivity in vincristine resistant cells showing multidrug resistance phenotypes in drug-free culture

Research Bulletin of Naruto University of Education


Prognosis-related gene expression in PBL, IL-2L, EBV-LCL, and wild type and drug resistant cultured cells


血小板凝集への白血球の関与

血小板凝集への白血球の関与

Bulletin of School of Medical Sciences, the University of Tokushima

徳島大学医療技術短期大学部紀要


Diagnostic accuracy of arrhythmias in semi-automatic analysis by Fukuda-Holter system(SCM-280)

ホルター心電計の不整脈診断精度に関する検討

徳島大学医療技術短期大学部紀要


血小板凝集能に及ぼすソマトスタチンの影響

血小板凝集能に及ぼすソマトスタチンの影響

徳島大学医療技術短期大学部紀要


臨床検査技師養成施設における基礎·臨床専門科目の実習に関する調査

臨床検査技師養成施設における基礎·臨床専門科目の実習に関する調査

全国の臨床検査技師養成施設を対象に(1)実習全般 (2)基礎専門科目の実習 (3)臨床専門科目の実習 (4)330時間の使用法の4部門16項目についてアンケート調査を実施し，約60%の施設から回答を得た．調査内容は実習の時間数，実習の内容，指導者，経費，機器，問題点，工夫している点などである．調査結果から各養成施設が学生の教育に真剣に取り組み，学生の卒後の成長，ひいては臨床検査学の将来のために非常な努力を払っていることがわかった．しかし，指導教官の不足，施設や機器の不十分，臨床実習のあり方など問題点なども多く，今後の改善が望まれる．

徳島大学医療技術短期大学部紀要


A family suspected of having Cis A2B phenotype

Bulletin of School of Medical Sciences, the University of Tokushima