read0017481/published_papers/41825088 Hiroshi Ito Yukihiro Numabe Shuichi Hashimoto Satoshi Sekino Etsuko Murakashi Hitomi Ishiguro Daisuke Sasaki Takashi Yaegashi Hideki Takai Masaru Mezawa Yorimasa Ogata Hisashi Watanabe Yuichi Izumi Jun-Ichi Kido Yuka Hiroshima Toshihiko Nagata Utility of a haemoglobin test of gingival crevicular fluid: A multicentre, observational study. OBJECTIVE: The purpose of this study was to verify the accuracy and utility of clinical parameters (plaque index, gingival crevicular fluid volume, probing depth, clinical attachment level, bleeding on probing and gingival index) and biochemical parameters (aspartate aminotransferase, protein and haemoglobin) in a longitudinal analysis during the supportive periodontal therapy period. SUBJECTS AND METHODS: A total of 279 test sites of 128 patients were investigated clinically and biochemically. After the first examination of clinical and biochemical parameters, periodontal support treatments were administered immediately and performed once every three months up to the second examination. RESULTS: All of the clinical and biochemical parameters were significantly lower at the second examination than at the first, except for the plaque index and bleeding on probing. Of these parameters, in particular, aspartate aminotransferase and haemoglobin in the gingival crevicular fluid were significantly reduced compared to those of the first examination in both the ≤4 and ≥5 mm probing depth groups, and they clearly suggested that periodontitis tended to recover. CONCLUSION: Adding the haemoglobin test to the bleeding on probing test strongly improves the accuracy of measurement of clinical parameters after periodontal treatment. Oral diseases null null 20230215 10.1111/odi.14536 36790046 read0017481/published_papers/35026214 Yuji Inagaki Jun-Ichi Kido Yasufumi Nishikawa Rie Kido Eijiro Sakamoto Mika Bando Koji Naruishi Toshihiko Nagata Hiromichi Yumoto Gan-Lu-Yin (Kanroin), Traditional Chinese Herbal Extracts, Reduces Osteoclast Differentiation In Vitro and Prevents Alveolar Bone Resorption in Rat Experimental Periodontitis Gan-Lu-Yin (GLY), a traditional Chinese herbal medicine, shows therapeutic effects on periodontitis, but that mechanism is not well known. This study aims to clarify the precise mechanism by investigating the inhibitory effects of GLY extracts on osteoclastogenesis in vitro and on bone resorption in periodontitis in vivo. RAW264.7 cells are cultured with soluble receptor activator of nuclear factor-kappa B (sRANKL) and GLY extracts (0.01-1.0 mg/mL), and stained for tartrate-resistant acid phosphatase (TRAP) to evaluate osteoclast differentiation. Experimental periodontitis is induced by placing a nylon ligature around the second maxillary molar in rats, and rats are administered GLY extracts (60 mg/kg) daily for 20 days. Their maxillae are collected on day 4 and 20, and the levels of alveolar bone resorption and osteoclast differentiation are estimated using micro-computed tomography (CT) and histological analysis, respectively. In RAW264.7 cells, GLY extracts significantly inhibit sRANKL-induced osteoclast differentiation at a concentration of more than 0.05 mg/mL. In experimental periodontitis, administering GLY extracts significantly decreases the number of TRAP-positive osteoclasts in the alveolar bone on day 4, and significantly inhibits the ligature-induced bone resorption on day 20. These results show that GLY extracts suppress bone resorption by inhibiting osteoclast differentiation in experimental periodontitis, suggesting that GLY extracts are potentially useful for oral care in periodontitis. Journal of clinical medicine 10 3 null null 20210120 10.3390/jcm10030386 33498415 read0017481/published_papers/26440929 Sakamoto E Kido JI Takagi R Inagaki Y Naruishi K Nagata T Yumoto H Advanced glycation end-product 2 and Porphyromonas gingivalis lipopolysaccharide increase sclerostin expression in mouse osteocyte-like cells. Bone 8756-3282 122 22 30 20190500 10.1016/j.bone.2019.02.001 30735798 read0017481/published_papers/26605175 Lew JH Naruishi K Kajiura Y Nishikawa Y Ikuta T Kido JI Nagata T High Glucose-Mediated Cytokine Regulation in Gingival Fibroblasts and THP-1 Macrophage: a Possible Mechanism of Severe Periodontitis with Diabetes. Diabetic conditions such as HG induce IL-1 and sIL-6R production from macrophages in inflammatory periodontal tissues and may exacerbate the periodontitis synergistically via MMP-1 production from HGFs. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 1015-8987 50 3 973 986 20181024 10.1159/000494481 30355945 read0017481/published_papers/26977865 Kohei Nonaka Yukari Kajiura Mika Bandou Eijiro Sakamoto Yuji Inagaki JH Lew Koji Naruishi Takahisa Ikuta Kaya Yoshida Tesuo Kobayashi Hiromasa Yoshie Toshihiko Nagata Jun-ichi Kido Advanced glycation end-products increase IL-6 and ICAM-1 expression via RAGE, MAPK and NF-kB pathways in human gingival fibroblasts Diabetes mellitus (DM) is a risk factor for periodontal diseases and may exacerbate the progression of the pathogenesis of periodontitis. Advanced glycation end-products (AGEs) cause DM complications relative to levels of glycemic control and larger amounts accumulate in the periodontal tissues of patients with periodontitis and DM. In the present study, we investigated the effects of AGEs on the expression of inflammation-related factors in human gingival fibroblasts (HGFs) to elucidate the impact of AGEs on DM-associated periodontitis. HGFs were cultured with or without AGEs. Cell viability was examined, and RNA and protein fractions were isolated from AGE-treated cells. The expression of interleukin (IL)-6, intercellular adhesion molecule-1 (ICAM-1), and the receptor for AGE (RAGE) was investigated using reverse transcription-polymerase chain reaction, quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, and reactive oxygen species activity was measured using a kit with 2&#039;,7&#039;-dichlorofluorescin diacetate. Human monocytic cells (THP-1) labeled with a fluorescent reagent were co-cultured with HGFs treated with AGEs and IL-6 siRNA, and the adhesive activity of THP-1 cells to HGFs was assessed. The expression of IL-6 and ICAM-1 was examined when HGFs were pretreated with recombinant human IL-6, the siRNAs of RAGE and IL-6, and inhibitors of MAPK and NF-κB, and then cultured with and without AGEs. The phosphorylation of MAPK and NF-κB was assessed using western blotting. AGEs increased the mRNA and protein expressions of RAGE, IL-6, ICAM-1 and reactive oxygen species activity in HGFs, and promoted the adhesion of THP-1 cells to HGFs, but had no effect on cell viability until 72 hours. Recombinant human IL-6 increased ICAM-1 expression in HGFs, while the siRNAs of RAGE and IL-6 inhibited AGE-induced IL6 and ICAM1 mRNA expression, and IL-6 siRNA depressed AGE-induced THP-1 cell adhesion. AGEs increased the phosphorylation of p38 and ERK MAPKs, p65 NF-κB and IκBα, while inhibitors of p38, ERK MAPKs and NF-κB significantly decreased AGE-induced IL-6 and ICAM-1 expression. AGEs increase IL-6 and ICAM-1 expression via the RAGE, MAPK and NF-κB pathways in HGFs and may exacerbate the progression of the pathogenesis of periodontal diseases. Journal of Periodontal Research 1600-0765 53 3 334 344 20180600 10.1111/jre.12518 29193068 read0017481/published_papers/26440925 K. Nonaka Nonaka K Y. Kajiura Kajiura Y M. Bando Bando M E. Sakamoto Sakamoto E Y. Inagaki Inagaki Y J. H. Lew Lew JH K. Naruishi Naruishi K T. Ikuta Ikuta T K. Yoshida Yoshida K T. Kobayashi Kobayashi T H. Yoshie Yoshie H T. Nagata Nagata T J. Kido Kido J Advanced glycation end-products increase IL-6 and ICAM-1 expression via RAGE, MAPK and NF-κB pathways in human gingival fibroblasts Advanced glycation end-products increase IL-6 and ICAM-1 expression via RAGE, MAPK and NF-κB pathways in human gingival fibroblasts. Journal of Periodontal Research Journal of periodontal research 1600-0765 53 3 334 344 20180600 10.1111/jre.12518 29193068 2-s2.0-85036593558 read0017481/published_papers/39576895 木戸 理恵 坂本 英次郎 板東 美香 稲垣 裕司 二宮 雅美 美原 智恵 生田 貴久 成石 浩司 永田 俊彦 木戸 淳一 湯本 浩通 インプラント周囲溝滲出液中のカルプロテクチンとNTxを用いたインプラント疾患の診断 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 60 132 132 20180500 read0017481/published_papers/26605180 Yukari Kajiura Jung-Hwan Lew Takahisa Ikuta Yasufumi Nishikawa Jun-Ichi Kido Toshihiko Nagata Koji Naruishi Clinical significance of GCF sIL-6R and calprotectin to evaluate the periodontal inflammation SAGE Publications Ltd Annals of Clinical Biochemistry 1758-1001 54 6 664 670 20171101 10.1177/0004563216680232 27810997 2-s2.0-85032428372 read0017481/published_papers/39576900 梶浦 由加里 二宮 雅美 生田 貴久 Lew Jung Hwan 木戸 淳一 永田 俊彦 上顎左右両側側切歯に認められた歯根嚢胞の一症例 (NPO)日本歯科保存学会 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 147 111 111 20171000 read0017481/published_papers/26605187 Yasufumi Nishikawa Yukari Kajiura Jung Hwan Lew Jun-ichi Kido Toshihiko Nagata Koji Naruishi Calprotectin Induces IL-6 and MCP-1 Production via Toll-Like Receptor 4 Signaling in Human Gingival Fibroblasts JOURNAL OF CELLULAR PHYSIOLOGY 1097-4652 232 7 1862 1871 20170700 10.1002/jcp.25724 27925202 read0017481/published_papers/26440911 J. Teramachi Y. Inagaki H. Shinohara H. Okamura D. Yang K. Ochiai R. Baba H. Morimoto T. Nagata T. Haneji PKR regulates LPS-induced osteoclast formation and bone destruction in vitro and in vivo Blackwell Publishing Ltd Oral Diseases 1601-0825 23 2 181 188 20170301 10.1111/odi.12592 27718290 2-s2.0-85005966819 read0017481/published_papers/26437584 Chie Mihara-Wada 美原(和田) 智恵 Koji Naruishi 成石 浩司 Mika Bandou 板東 美香 Nishikawa Yasufumi 西川 泰史 Jung-Hwan Lew Eijiro Sakamoto 坂本 英次郎 Ikuta Takahisa 生田 貴久 Kono Kaoru 河野 薫 Yukari Kajiura 梶浦 由加里 Hashimoto Mari 橋本 万里 Yukiko Nakajima 中島 由紀子 Yuji Inagaki 稲垣 裕司 Masami Ninomiya 二宮 雅美 Jun-ichi Kido 木戸 淳一 Toshihiko Nagata 永田 俊彦 Post clinical clerkship-OSCEを見据えた歯周病科での模擬OSCEの概要 Journal of Oral Health and Biosciences 2188-7888 29 2 49 53 20170200 10.20738/johb.29.2_49 read0017481/published_papers/26437600 Jun-ichi Kido 木戸 淳一 Mika Bandou 板東 美香 Yuji Inagaki 稲垣 裕司 生田 貴久 Yukari Kajiura 梶浦 由加里 Yukiko Nakajima 中島 由紀子 Eijiro Sakamoto 坂本 英次郎 Chie Mihara-Wada 美原(和田) 智恵 Masami Ninomiya 二宮 雅美 西川 泰史 Jung-Hwan Lew Koji Naruishi 成石 浩司 Toshihiko Nagata 永田 俊彦 Diagnosis of Periodontal Diseases using Biomarkers in Gingival Crevicular Fluid 歯肉溝滲出液中のバイオマーカーを用いた歯周病診断 Periodontal diseases cause an inflammation and degradation of periodontal tissues and missing of teeth. The incidence rate of periodontal diseases is high in middle-aged and elderly people.A reasonable diagnosis of periodontal diseases is very important to keep teeth, however, conventional examinations of periodontal diseases is not necessarily exact and objective. Gingival crevicular fluid (GCF) is an exudate secreted from periodontal tissues and contains many components including proteolytic enzymes, inflammatory cytokines, blood-associated proteins, cellular and bacterial fragments. Because some proteins in GCF are related to inflammation, tissue degradation and bone metabolism, those proteins have been studying as a diagnostic marker of periodontal diseases. GCF is noninvasively collected using a sterile paper strip and biomarkers are determined using enzyme-linked immunosorbent assay (ELISA) and enzyme activity assay. We identified calprotectin, an inflammationrelated protein, in GCF and calprotectin level in GCF from periodontitis sites was significantly higher than that of healthy control. Calprotectin level in GCF was positively correlated to gingival index and other biomarkers and decreased by periodontal treatments. Resistin is an adipocytokine and its level increases in some inflammatory diseases. Resistin level in GCF from periodontitis sites was high compared to the level of healthy control samples. Procollagen type I C-terminal peptide (PICP) is a biomarker for bone metabolism and its level was high in GCF collected from periodontitis sites. These results suggested that calprotectin, resistin and PICP are useful biomarkers for periodontal diseases. On the other hand, we showed that glycated albumin (GA), a marker of diabetes mellitus (DM), was contained in GCF and GA level in GCF from DM patients was significantly higher than that of non-DM individuals. Components in GCF may be biomarkers of systemic diseases as well as periodontal diseases and their determination will be useful diagnostic examination of some diseases. Recently, we have been studying the determining system of GCF calprotectin, including microchip ELISA, surface plasmon resonance assay and immuno-chromatography assay. When GCF biomarkers are determined using the determining systems, we will simply, exactly and objectively diagnose periodontal diseases at our dental offices. Journal of Oral Health and Biosciences 2188-7888 29 1 13 20 20161200 read0017481/published_papers/26437587 Koji Naruishi Keiji Oishi Yuuji Inagaki Masumi Horibe Mika Bandou Masami Ninomiya K Kawahara J Minakuchi S Kawashima K Shima Jun-ichi Kido Toshihiko Nagata Association between Periodontal Condition and Kidney Dysfunction in Japanese Adults: A Cross-Sectional Study Clinical and Experimental Dental Research 2057-4347 2 2 1 8 20160610 10.1002/cre2.39 read0017481/published_papers/26440905 Eijiro Sakamoto Chie Mihara-Wada Takahisa Ikuta Yuji Inagaki Jun-ichi Kido Toshihiko Nagata Inhibitory effects of advanced glycation end-products and Porphyromonas gingivalis lipopolysaccharide on the expression of osteoblastic markers of rat bone marrow cells in culture Diabetes is a major risk factor for periodontitis and there is a close relationship between the degree of hyperglycemia and the severity of periodontitis. Advanced glycation end-products (AGEs) accumulate in various tissues under diabetic conditions. AGEs in the periodontal tissues probably play a role in upregulating periodontal inflammation; however, the association of AGEs with the severity of periodontitis has not been fully clarified. Lipopolysaccharide from Porphyromonas gingivalis (P-LPS) is a potent pathogenic factor in periodontitis. Although the independent effect of AGE or P-LPS on osteoblastic cells has been reported in vitro, the effect of adding both has not been clearly elucidated. In this study, to explore factors aggravating diabetic periodontitis, we investigated the effects of AGE and P-LPS on the expression of osteoblastic markers and the expression of inflammation-related markers in vitro. Rat bone marrow cells were cultured, and alkaline phosphatase activity and bone nodule formation were evaluated as osteoblastic markers. Reverse transcription-polymerase chain reaction was performed to determine the mRNA expression of molecules associated with bone and inflammation. Protein levels of osteocalcin and interleukin-1β (IL-1β) were measured using enzyme-linked immunosorbent assay. AGEs and P-LPS independently reduced alkaline phosphatase activity and bone nodule formation. The addition of both AGE and P-LPS (AGE+P-LPS) further decreased these markers. Reverse transcription-polymerase chain reaction analysis revealed that AGE+P-LPS markedly decreased the mRNA expression of osteoblast-related molecules such as type 1 collagen, osteocalcin and Cbfa1, and markedly increased that of inflammation-related molecules such as IL1β and S100A8. AGE and P-LPS decreased the protein level of osteocalcin and increased that of IL-1β, and a further increase of IL-1β was detected for AGE+P-LPS. AGEs and P-LPS inhibited the expression of osteoblastic markers and increased the levels of inflammatory markers in rat bone marrow cells, suggesting that both AGE and P-LPS may be important factors associated with the aggravation of diabetic periodontitis. Journal of Periodontal Research 1600-0765 51 3 313 320 20160600 10.1111/jre.12310 26223811 read0017481/published_papers/26440869 Yuka Hiroshima Mika Bando Yuji Inagaki Reiko Kido Masatoshi Kataoka Toshihiko Nagata Jun-ichi Kido Effect of Hangeshashinto on calprotectin expression in human oral epithelial cells Springer-Verlag Tokyo Odontology 1618-1255 104 2 152 162 20160501 10.1007/s10266-015-0196-3 25649126 2-s2.0-84922161650 read0017481/published_papers/26440867 Jun-Ichi Kido Y. Bando M. Bando Y. Kajiura Y. Hiroshima Y. Inagaki H. Murata T. Ikuta R. Kido K. Naruishi M. Funaki T. Nagata YKL-40 level in gingival crevicular fluid from patients with periodontitis and type 2 diabetes Blackwell Publishing Ltd Oral Diseases 1601-0825 21 5 667 673 20150701 10.1111/odi.12334 25740558 2-s2.0-84930271794 read0017481/published_papers/26440865 Koji Naruishi 成石 浩司 Yukari Kajiura 梶浦 由加里 西川 泰史 Mika Bandou 板東 美香 Jun-ichi Kido 木戸 淳一 Toshihiko Nagata 永田 俊彦 Inhibitory Effects of Cryptotanshinone on Inflammation-related Molecules in Human Gingival Fibroblasts 培養歯肉線維芽細胞におけるクリプトタンシノンによる炎症関連分子の産生抑制効果 Purpose: Cryptotanshinone (CPT) isolated from the root of an Asian medicinal plant, Salvia miltiorrhiza bunge, acts as a potent anti-tumor or anti-inflammatory agent in vitro. However, the effects of CPT on the progression of periodontitis are still unknown. This study investigated the effects of CPT on the production of inflammation-related molecules in human gingival fibroblasts. Methods: Human gingival fibroblasts (HGFs), CRL-2014TM (ATCC), were used. Cytotoxicity of the cells by CPT was examined. Dead cells stained by trypan blue were counted and analyzed. Next, the levels of IL-6 and VEGF in IL-1β-treated cells with or without CPT were measured using ELISA kits. The productivity of cathepsin L in IL-1β-treated cells with or without CPT was examined using Western blotting. Results: The ratio of viable cell numbers was significantly decreased by 100 μmol/l CPT. Next, the increase of IL-6 and cathepsin L induced by IL-1β was significantly suppressed by the pretreatment of 10 μmol/l CPT in HGFs. On the other hand, productivity of VEGF induced by IL-1β was not changed by the pretreatment of 10 μmol/l CPT. Conclusion: CPT suppresses IL-1β-induced IL-6 and cathepsin L production in HGFs, and might regulate the progression of periodontitis. 目的 : 漢方薬 (生薬) タンジンの主成分であるクリプトタンシノンは, 抗がん作用や抗炎症作用をもつことが知られている. しかしながら, クリプトタンシノンが歯周組織の炎症に対してどのような影響を及ぼすのかは不明である. 本研究の目的は, 歯肉線維芽細胞を標的として, その炎症関連分子の産生に及ぼすクリプトタンシノンの影響を調べることである. 方法 : 細胞は歯肉線維芽細胞CRL-2014TM (ATCC) を用いた. クリプトタンシノンの濃度依存的な細胞障害作用の有無は, トリパンブルー染色法を用いて各濃度間における生細胞率の差を比較検討して評価した. 歯肉線維芽細胞のサイトカイン産生は, 市販のELISAキットを用いて, インターロイキン (IL) -1βで細胞を24時間刺激した後のIL-6およびVEGF産生量を測定して検討した. 一方, タンパク質分解酵素カテプシンLの産生は, IL-1βで細胞を24時間刺激した後に回収した全細胞タンパク質を用いて, ウエスタンブロット法によって検討した. なお, クリプトタンシノンはIL-1β添加の1時間前に細胞培養系に添加した. 各群における差異は, ANOVA Tukey-KramerのHSD検定によって有意差を検討した. 結果 : クリプトタンシノンは, 10μmol/lまでは歯肉線維芽細胞の生細胞率に影響は与えないが, 100μmol/lでは有意に生細胞率を低下させた. また, 歯肉線維芽細胞におけるIL-1β誘導性のIL-6産生およびカテプシンL産生は, 10μmol/lクリプトタンシノンの添加によって著明に減少した. 一方, IL-1β誘導性のVEGF産生は, クリプトタンシノンの添加によっても変化しなかった. 結論 : クリプトタンシノンは, 歯肉線維芽細胞におけるIL-1β誘導性IL-6およびカテプシンLの産生を抑制することで, 歯周組織の炎症を抑制する可能性が示唆された. The Japanese Journal of Conservative Dentistry 日本歯科保存学雑誌 0387-2343 58 3 212 218 20150600 10.11471/shikahozon.58.212 read0017481/published_papers/26440916 Y. Nakajima Y. Inagaki J. Kido T. Nagata Advanced glycation end products increase expression of S100A8 and A9 via RAGE-MAPK in rat dental pulp cells Blackwell Publishing Ltd Oral Diseases 1601-0825 21 3 328 334 20150401 10.1111/odi.12280 25098709 2-s2.0-84925124937 read0017481/published_papers/7980396 Takahisa Ikuta Ikuta T Yuji Inagaki Inagaki Y Kazuya Tanaka Tanaka K Tsuyoshi Saito Saito T Yukiko Nakajima Nakajima Y Mika Bando Bando M Jun-ichi Kido Kido JI Toshihiko Nagata Nagata T Gene polymorphism of beta-defensin-1 is associated with susceptibility to periodontitis in Japanese Gene polymorphism of β-defensin-1 is associated with susceptibility to periodontitis in Japanese. ODONTOLOGY Odontology / the Society of the Nippon Dental University 1618-1255 103 1 66 74 20150100 10.1007/s10266-013-0139-9 24276427 read0017481/published_papers/26440914 Yuji Inagaki 稲垣 裕司 Yukiko Nakajima 中島 由紀子 Masumi Horibe 堀部 ますみ 生田 貴久 Yukari Kajiura 梶浦 由加里 Koji Naruishi 成石 浩司 Jun-ichi Kido 木戸 淳一 Toshihiko Nagata 永田 俊彦 肥満糖尿病ラット歯周炎の歯肉組織におけるオステオポンチンの局在 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 67 4 149 158 20150000 10.2329/perio.57.149 read0017481/published_papers/26440873 Yukari Kajiura Mika Bandou Yuji Inagaki Toshihiko Nagata Jun-ichi Kido Glycated albumin and calprotectin levels in gingival crevicular fluid from patients with periodontitis and type 2 diabetes Patients with diabetes mellitus (DM) have a high prevalence of periodontitis. Periodontitis in these patients is characterized by severe inflammation and tissue breakdown, and its diagnosis is important for cures of periodontitis and DM. The purpose of this study is to investigate the levels of glycated albumin (GA), a DM marker, and calprotectin, an inflammatory marker, in gingival crevicular fluid (GCF) from patients with periodontitis and DM (DM-P). The 78 participants in this study were patients with DM, chronic periodontitis (CP), DM-P, and healthy individuals (H). GCF and blood were collected, and GA and calprotectin in GCF were analyzed using Western blotting and enzyme-linked immunosorbent assay. Levels were compared among H, DM, CP, and DM-P groups. Blood GA and glycated hemoglobin (HbA1c) were measured, and the correlation among GCF GA and blood HbA1c or GA levels was investigated. Receiver operating characteristic (ROC) analysis for GCF GA to predict DM was performed. GA was identified in GCF, and its amount/concentration in GCF samples from DM and DM-P were significantly higher than those of non-DM groups (H and CP). Calprotectin amounts in GCF from CP and DM-P were significantly higher than in H and DM groups. GCF GA level was positively correlated with blood HbA1c and GA level. ROC analysis of GCF GA showed an optimal cutoff value to predict DM. GA showed a high level in GCF from patients with DM. Examination of GA and calprotectin in GCF may be useful for predicting DM-P. Journal of Periodontology 1943-3670 85 12 1667 1675 20141200 10.1902/jop.2014.140241 25126693 read0017481/published_papers/39576917 梶浦 由加里 坂東 由記子 木戸 淳一 板東 美香 稲垣 裕司 中島 由紀子 生田 貴久 村田 裕美 廣島 佑香 成石 浩司 永田 俊彦 歯周病および糖尿病患者の歯肉溝滲出液中のYKL-40レベルの検討 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 56 124 124 20140900 read0017481/published_papers/39576922 梶浦 由加里 板東 美香 木戸 淳一 稲垣 裕司 生田 貴久 橋本 万里 篠原 宏貴 二宮 雅美 村田 裕美 中島 由紀子 米田 哲 美原 智恵 廣島 佑香 大石 慶二 永田 俊彦 歯肉溝浸出液中グリコアルブミンおよびカルプロテクチンを指標とした糖尿病関連歯周炎の診断 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 56 104 104 20140400 read0017481/published_papers/7980400 Hiroshi Ito Yukihiro Numabe Satoshi Sekino Etsuko Murakashi Hitomi Iguchi Shuichi Hashimoto Daisuke Sasaki Takashi Yaegashi Kazushi Kunimatsu Hideki Takai Masaru Mezawa Yorimasa Ogata Hisashi Watanabe Satsuki Hagiwara Yuichi Izumi Yuka Hiroshima Jun-Ichi Kido Toshihiko Nagata Evaluation of bleeding on probing and gingival crevicular fluid enzyme activity for detection of periodontally active sites during supportive periodontal therapy ODONTOLOGY 1618-1255 102 1 50 56 20140100 10.1007/s10266-012-0090-1 23179356 read0017481/published_papers/7980399 Yoshikazu Fukumoto Masumi Horibe Yuji Inagaki Keiji Oishi Naofumi Tamaki Hiro-O Ito Toshihiko Nagata Association of gingival recession and other factors with the presence of dentin hypersensitivity ODONTOLOGY 1618-1255 102 1 42 49 20140100 10.1007/s10266-012-0099-5 23283584 read0017481/published_papers/26027696 Yoshikazu Fukumoto Masumi Horibe Yuji Inagaki Keiji Oishi Naofumi Tamaki Hiro-O Ito Toshihiko Nagata Association of gingival recession and other factors with the presence of dentin hypersensitivity Odontology 1618-1247 102 1 42 49 20140000 10.1007/s10266-012-0099-5 23283584 2-s2.0-84892873887 read0017481/published_papers/26027682 Naofumi Tamaki Rita Cristina Orihuela-Campos Yuji Inagaki Makoto Fukui Toshihiko Nagata Hiro-O Ito Resveratrol improves oxidative stress and prevents the progression of periodontitis via the activation of the Sirt1/AMPK and the Nrf2/antioxidant defense pathways in a rat periodontitis model Elsevier Inc. Free Radical Biology and Medicine 1873-4596 75 222 229 20140000 10.1016/j.freeradbiomed.2014.07.034 25091897 2-s2.0-84907345449 read0017481/published_papers/26437589 Masami Ninomiya Tomoko Azuma Jun-ichi Kido Shinya Murakami Toshihiko Nagata Successful case of periodontal tissue repair by fibroblast growth factor-2. -Long-term follow-up and comparison with enamel matrix derivative- Clinical Advances in Periodontics 2163-0097 3 4 216 221 20131100 10.1902/cap.2012.120014 read0017481/published_papers/39576926 生田 貴久 稲垣 裕司 中島 由紀子 板東 美香 木戸 淳一 永田 俊彦 日本人歯周病患者を対象とした抗菌ペプチド遺伝子のSNPs解析と疾患感受性の検討 (NPO)日本歯科保存学会 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 139 49 49 20131000 read0017481/published_papers/7980398 Bando M Zou X Hiroshima Y Kataoka M Ross KF Shinohara Y Nagata T Herzberg MC Kido J Mechanism of interleukin-1α transcriptional regulation of S100A9 in a human epidermal keratinocyte cell line. 9 Biochimica et biophysica acta 0006-3002 1829 954 962 20130900 10.1016/j.bbagrm.2013.03.010 23563247 read0017481/published_papers/35202160 村田 裕美 前田 博史 高柴 正悟 木戸 淳一 永田 俊彦 神経変性疾患関連因子TDP-43は単球におけるLPS誘導性TNF-α転写制御に関与する (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 55 107 107 20130900 read0017481/published_papers/26440876 Mika Bando Mika Bandou Xianqiong Zou Yuka Hiroshima Masatoshi Kataoka Karen F. Ross Yasuo Shinohara Toshihiko Nagata Mark C. Herzberg Jun-ichi Kido Mechanism of interleukin-1α transcriptional regulation of S100A9 in a human epidermal keratinocyte cell line Biochimica et Biophysica Acta - Gene Regulatory Mechanisms Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms 1874-9399 1829 9 954 962 20130900 10.1016/j.bbagrm.2013.03.010 23563247 2-s2.0-84880346974 read0017481/published_papers/7980397 Yukiko Nakajima Yuji Inagaki Yuka Hiroshima Jun-ichi Kido Toshihiko Nagata Advanced Glycation End-products Enhance Calcification in Cultured Rat Dental Pulp Cells JOURNAL OF ENDODONTICS 0099-2399 39 7 873 878 20130700 10.1016/j.joen.2012.11.027 23791254 read0017481/published_papers/39576934 二宮 雅美 板東 美香 村田 裕美 生田 貴久 木戸 淳一 永田 俊彦 姉妹に認められた広汎型侵襲性歯周炎に対して包括的歯周治療を行った症例 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 55 129 129 20130400 read0017481/published_papers/39576929 木戸 淳一 板東 美香 坂東 由記子 稲垣 裕司 廣島 佑香 梶浦 由加里 美原 智恵 村田 裕美 生田 貴久 篠原 宏貴 橋本 万里 船木 真理 齋藤 晴比古 永田 俊彦 糖尿病関連歯周炎の診断マーカーとしての歯肉溝滲出液中グリコアルブミンとカルプロテクチンの有用性 (一社)日本糖尿病学会 糖尿病 1881-588X 56 Suppl.1 S 220 20130400 read0017481/published_papers/26437602 Masami Ninomiya 二宮 雅美 Toshihiko Nagata 永田 俊彦 FGF-2による歯周組織再生療法 歯の長期保存の臨床 私はこうして歯を守る 38 6 126 131 20130300 read0017481/published_papers/26440918 Yukiko Nakajima Yuji Inagaki Yuka Hiroshima Jun-ichi Kido Toshihiko Nagata Advanced glycation end-products enhance calcification in cultured rat dental pulp cells Amorphous calcification frequently appears in dental pulp tissues of diabetic patients; however, its pathologic process has not been fully elucidated. We previously found that pulp stones and thickened predentin occurred more frequently in diabetic rats. Recent findings demonstrated that accumulation of advanced glycation end-products (AGE) might be involved in vascular calcification complicated with diabetes. The aim of this study was to determine the effect of AGE on calcified nodule formation by rat dental pulp cells in culture. Rat dental pulp cells and gingival fibroblasts were independently cultured with 50 and 100 μg/mL AGE. Alkaline phosphatase activity and calcified nodule formation were measured. Expressions of receptor for AGE, osteopontin (OPN), and osteocalcin (OCN) mRNA were determined by quantitative real-time polymerase chain reaction. Protein levels of OPN and OCN secreted in culture medium were quantified by enzyme-linked immunosorbent assay. AGE (50 and 100 μg/mL) markedly increased both alkaline phosphatase activity and calcified nodule formation in dental pulp cells (P &lt; .01), whereas it did not affect those in gingival fibroblasts. Real-time polymerase chain reaction analysis revealed that AGE increased mRNA expressions of receptor for AGE, OPN, and OCN in dental pulp cells (P &lt; .05). Enzyme-linked immunosorbent assay analysis revealed that the protein levels of OPN and OCN produced by dental pulp cells were higher in AGE-treated than in untreated cells (P &lt; .05). AGE enhanced the calcification potentials of rat dental pulp cells, suggesting that it may stimulate pathologic calcification of diabetic dental pulp tissues. Journal of Endodontics 1878-3554 39 873 878 20130116 10.1016/j.joen.2012.11.027 23791254 read0017481/published_papers/26440871 Takahisa Ikuta Yuji Inagaki Kazuya Tanaka Tsuyoshi Saito Yukiko Nakajima Mika Bando Mika Bandou Jun-ichi Kido Toshihiko Nagata Gene polymorphism of β-defensin-1 is associated with susceptibility to periodontitis in Japanese Gene polymorphism of β-defensin-1 is associated with susceptibility to periodontitis in Japanese. Springer Tokyo Odontology 1618-1255 103 1 66 74 20130000 10.1007/s10266-013-0139-9 24276427 2-s2.0-84887927465 read0017481/published_papers/7980401 Jun-ichi Kido Kaori Abe Shouki Yatsushiro Mika Bando Yuka Hiroshima Toshihiko Nagata Toshihiko Ooie Masato Tanaka Masatoshi Kataoka Determination of calprotectin in gingival crevicular fluid by immunoassay on a microchip CLINICAL BIOCHEMISTRY 0009-9120 45 15 1239 1244 20121000 10.1016/j.clinbiochem.2012.05.009 22609893 read0017481/published_papers/39581605 稲垣 裕司 斉藤 剛 田中 和也 生田 貴久 中島 由紀子 木戸 淳一 永田 俊彦 日本人歯周病患者を対象とした抗菌ペプチド遺伝子のSNPs解析と疾患感受性の検討 (NPO)日本歯科保存学会 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 137 64 64 20121000 read0017481/published_papers/39581602 堀部 ますみ 稲垣 裕司 中島 由紀子 生田 貴久 木戸 淳一 永田 俊彦 糖尿病ラットの実験的歯周炎における歯周組織破壊とオステオポンチンの局在 (NPO)日本歯科保存学会 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 137 157 157 20121000 read0017481/published_papers/26440880 Y. Hiroshima M. Bando Y. Inagaki C. Mihara M. Kataoka H. Murata Y. Shinohara T. Nagata J. Kido Resistin in gingival crevicular fluid and induction of resistin release by Porphyromonas gingivalis lipopolysaccharide in human neutrophils Journal of Periodontal Research 0022-3484 47 5 554 562 20121000 10.1111/j.1600-0765.2011.01466.x 22309231 2-s2.0-84865553635 read0017481/published_papers/26440878 Jun-Ichi Kido Kaori Abe Shouki Yatsushiro Mika Bando Yuka Hiroshima Toshihiko Nagata Toshihiko Ooie Masato Tanaka Masatoshi Kataoka Determination of calprotectin in gingival crevicular fluid by immunoassay on a microchip Clinical Biochemistry 0009-9120 45 15 1239 1244 20121000 10.1016/j.clinbiochem.2012.05.009 22609893 2-s2.0-84866267825 read0017481/published_papers/39581609 生田 貴久 稲垣 裕司 坂本 英次郎 中島 由紀子 木戸 淳一 永田 俊彦 模擬無重力下での培養骨芽細胞の分化発現に及ぼす歯周病原性因子の影響 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 54 86 86 20120900 read0017481/published_papers/26440882 J. Kido M. Bando Y. Hiroshima H. Iwasaka K. Yamada N. Ohgami T. Nambu M. Kataoka T. Yamamoto Y. Shinohara I. Sagawa T. Nagata Analysis of proteins in human gingival crevicular fluid by mass spectrometry Journal of Periodontal Research 0022-3484 47 4 488 499 20120800 10.1111/j.1600-0765.2011.01458.x 22220998 2-s2.0-84862893533 read0017481/published_papers/39581612 二宮 雅美 生田 貴久 永田 俊彦 下顎前歯部の広範囲に認められた歯根嚢胞の一症例 (NPO)日本歯科保存学会 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 136 151 151 20120500 read0017481/published_papers/26927012 Purevjav Javkhlan Yuka Hiroshima Ahmad Azlina Takahiro Hasegawa Chenjuan Yao Tetsuya Akamatsu Jun-ichi Kido Toshihiko Nagata Kazuo Hosoi Lipopolysaccharide-Mediated Induction of Calprotectin in the Submandibular and Parotid Glands of Mice INFLAMMATION 0360-3997 34 6 668 680 20111200 10.1007/s10753-010-9277-1 21125321 read0017481/published_papers/26440884 Yuka Hiroshima Mika Bandou Masatoshi Kataoka Yuji Inagaki Mark C. Herzberg Karen F. Ross Kazuo Hosoi Toshihiko Nagata Jun-ichi Kido Regulation of antimicrobial peptide expression in human gingival keratinocytes by interleukin-1α In the oral cavity, mucosal keratinocytes resist bacterial infection, in part, by producing broad-spectrum antimicrobial peptides (AMPs) including defensin, adrenomedullin and calprotectin. Epidermal keratinocyte expression of many AMPs increases in response to interleukin-1α (IL-1α). IL-1α is produced by epidermal keratinocytes and regulates cell differentiation. To better understand innate immunity in the oral cavity, we sought to determine how IL-1α might regulate expression of AMPs by human gingival keratinocytes (HGKs) using DNA microarray and Western blot analyses. HGKs from three subjects expressed eleven AMPs, including S100A7, S100A8, S100A9, S100A12, secretory leucocyte protease inhibitor, lipocalin 2 (LCN2), cystatin C and β-defensin 2. Of the expressed AMPs, S100A7, S100A12 and LCN2 were up-regulated by IL-1α (inducible AMPs); the other AMPs were considered to be constitutive. Human gingival keratinocytes, therefore, express constitutive and IL-1α-inducible AMPs to provide a rapid and robust innate response to microbial infection. Archives of Oral Biology 1879-1506 56 8 761 767 20110211 10.1016/j.archoralbio.2011.01.004 21316034 read0017481/published_papers/39306444 Hiroshima Yuka 廣島 佑香 Bando Mika 板東 美香 Kido Jun-ichi 木戸 淳一 Kataoka Masatoshi 片岡 正俊 Nagata Toshihiko 永田 俊彦 The effect of Hangeshashinto on calprotectin expression in human oral epithelial cells ヒト口腔上皮細胞におけるカルプロテクチン発現に及ぼす半夏瀉心湯の影響 JAPANESE SOCIETY OF PERIODONTOLOGY 特定非営利活動法人 日本歯周病学会 Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology 特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集 2011 79 79 20110000 10.14833/amjsp.2011s.0.79.0 read0017481/published_papers/26440886 Yuji Inagaki 稲垣 裕司 Mika Bandou 板東 美香 中島 由紀子 Yuka Hiroshima 廣島 佑香 Jun-ichi Kido 木戸 淳一 Toshihiko Nagata 永田 俊彦 Osmotic Stress Affects Calcium Deposition and Osteopontin Expression in Cultured Rat Dental Pulp Cells オスモティックストレスが培養歯髄細胞の硬組織形成能とオステオポンチン産生に及ぼす影響 Osmotic stress is a stimulus caused by osmotic pressure, which is known to affect physiological functions in various tissues and cells. However, there is little information on the effect of osmotic stress on dental pulp tissues and cells. In this study, we investigated the relationship between osmotic stress and calcium deposition in cultured rat dental pulp cells. We also analyzed the effect of osmotic stress on the expression and phosphorylation of osteopontin (OPN), a multi-functional bone matrix protein, in the dental pulp cells. Both hypo- and hyper-osmotic stress affected cell-shape and -growth, and decreased calcium deposition in cultured rat dental pulp cells. Hypoosmotic stress increased OPN secretion and its protein ratio in the culture medium. Also, the secreted OPN was highly phosphorylated. On the other hand, hyper-osmotic stress decreased OPN secretion and its protein ratio and most of the OPN in the hypertoinc medium was dephosphorylated. Both hypo- and hyper-osmotic stress decreased calcium deposition in the cultured rat dental pulp cells. However, the mechanism of the inhibitory action seemed to be different in the two groups, because the amount and phosphorylation pattern of OPN were not similar between the hypo- and hyper-osmotic conditions. We concluded that osmotic stress affects calcium deposition in cultured rat dental pulp cells and that OPN expression and its phosphorylation may be a control factor of osmotic stress-associated mineralization. 浸透圧変化が惹起する刺激源は一般にオスモティックストレス(osmotic stress)と呼ばれており,種々の組織や細胞に影響をもたらすことが知られている.しかし,オスモティックストレスが歯髄の組織や細胞にどのような影響を与えるのかを論じた成書や研究報告はぼとんどない.本研究ではラット由来の歯髄細胞培養系を用いて,オスモティックストレスと歯髄細胞の硬組織形成の関連性を検証した.また,オスモティックストレスによって硬組織形成関連タンパクであるオステオポンチン(OPN)の発現やリン酸化に変化が生じるかも検証した.その結果,歯髄細胞培養系においては,低張および高張のいずれのオスモティックストレスによっても細胞の形態や増殖に影響が認められ,硬組織形成も低下した.低張ストレスを付与するとOPNの発現量や総タンパク量に対するOPNの比率は上昇し,また発現したOPNはリン酸化されていた.一方,高張ストレスを付与するとOPNの発現量や総タンパク量に対するOPNの比率は低下し,脱リン酸化されたOPNが主に発現した.このように低張と高張のオスモティックストレスではOPNの発現量やリン酸化による修飾の程度が異なっていたことから,低張および高張のいずれのオスモティックストレスによっても石灰化は抑制されたが,その抑制のメカニズムは一様でないことが明らかとなった.以上より,オスモティックストレスは歯髄細胞の硬組織形成に影響を与え,OPNの発現およびそのリン酸化が制御因子として機能している可能性が示唆された. The Japanese Society of Conservative Dentistry 特定非営利活動法人日本歯科保存学会 The Japanese Journal of Conservative Dentistry 日本歯科保存学雑誌 0387-2343 53 4 367 375 20100831 10.11471/shikahozon.53.367 read0017481/published_papers/26440923 Yuji Inagaki Kaya Yoshida Hirofumi Ohba Hiroyuki Seto Jun-ichi Kido Tatsuji Haneji Toshihiko Nagata High glucose levels increase osteopontin production and pathologic calcification in rat dental pulp tissues. INTRODUCTION: Pulp stones are frequently formed as a pathologic calcification product in dental pulp tissues, but the pathogenesis is poorly understood. We previously found that osteopontin (OPN) was produced by dental pulp cells, and its expression was associated with formation of the pulp stone matrix. It was reported that amorphous calcification appeared in the dental pulp of diabetic patients. The aim of this study was to determine the relationship between OPN expression and pathologic calcification in rat diabetic pulp. METHODS: The effect of glucose on OPN production and alkaline phosphatase activity in cultured rat dental pulp cells (RPC-C2A) was investigated, and then dental pulp calcification and OPN expression in diabetic rats were determined and compared with those in healthy rats by histologic and immunohistochemical analyses. RESULTS: In RPC-C2A cells, biochemical analysis showed that a high concentration of glucose (50 mmol/L) increased OPN protein production and alkaline phosphatase activity 1.3-fold and 1.5-fold, respectively. Histologic observations showed more calcified particles in dental pulp tissues in diabetic than in nondiabetic rats. Moreover, a thickened layer of predentin was formed in the radicular pulp of diabetic rats. OPN was more strongly stained around the calcified particles and in the odontoblast zone under the thickened predentin in diabetic rats. CONCLUSIONS: OPN might be a key molecule involved in the increase of pathologic pulp calcifications, which are frequently observed in diabetic patients. Journal of Endodontics 1878-3554 36 6 1014 1020 20100424 10.1016/j.joen.2010.03.018 20478457 read0017481/published_papers/26440891 Mika Bando Yuka Hiroshima Masatoshi Kataoka Mark C Herzberg Karen F Ross Yasuo Shinohara Takenori Yamamoto Toshihiko Nagata Jun-Ichi Kido Modulation of calprotectin in human keratinocytes by keratinocyte growth factor and interleukin-1α Immunology and Cell Biology 0818-9641 88 3 328 333 20100300 10.1038/icb.2009.104 20065999 2-s2.0-77949874587 read0017481/published_papers/26440888 Y. Hiroshima M. Bando M. Kataoka Y. Shinohara M. C. Herzberg K. F. Ross Y. Inagaki T. Nagata J. Kido Shosaikoto increases calprotectin expression in human oral epithelial cells Journal of Periodontal Research 0022-3484 45 1 79 86 20100200 10.1111/j.1600-0765.2009.01203.x 19602113 2-s2.0-74549212506 read0017481/published_papers/39306343 Hiroshima Yuka 廣島 佑香 Bando Mika 板東 美香 Kido Jun-ichi 木戸 淳一 Kataoka Masatoshi 片岡 正俊 Nagata Toshihiko 永田 俊彦 Microarray analysis of antimicrobial peptide gene expression in human gingival keratinocytes ヒト歯肉上皮細胞における抗菌ペプチド遺伝子発現のマイクロアレイ法による解析 JAPANESE SOCIETY OF PERIODONTOLOGY 特定非営利活動法人 日本歯周病学会 Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology 特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集 2009 29 29 20090000 10.14833/amjsp.2009f.0.29.0 read0017481/published_papers/26440894 Yuka Hiroshima 廣島 佑香 Yuji Inagaki 稲垣 裕司 Mika Bandou 板東 美香 Jun-ichi Kido 木戸 淳一 Toshihiko Nagata 永田 俊彦 Long-term Observation of Marked Overfilling in Root Canal Therapy : A Report of Three Cases 著明な過剰根管充填の経過を長期観察した3症例 In endodontic therapy, overfilling of gutta-percha point in root canals sometimes occurs due to wrong measurement of working length and overextension of gutta-percha point. Although a slight overfilling to periapical tissues (within 2 mm past the apical foramen) is tolerable, marked overfilling should be checked by postoperative recheck radiographs and/or clinical symptoms. In this report, three clinical cases of marked overfilling are described and long-term radiographic observation (6-14 years) was continued. Overfilled gutta-percha points on the radiographic observation were 3.0 (case 1), 2.5 (case 2), and 3.5 mm (case 3) in the left maxillary molar of a 47-year-old woman, in the left mandibular molar of a 45-year-old woman, and in the left mandibular premolar of a 39-year-old woman, respectively. In case 1, an overfilled point was cut after 1.5 years and disappeared after 3.5 years, and then apical radiolucency was improved after 6 years. In case 2, overfilled points started to minimize after 3 years and disappeared after 14 years. In case 3, an overfilled point was minimized after 13 years accompanied with improvement of apical lesion. None of the cases showed any significant clinical symptoms after the overfilling. These observations are interesting to longitudinally evaluate the disappearance of overfilled gutta-percha and the improvement of apical lesion. 歯内治療において,ときどき過剰根管充填(過剰根充)に遭遇する.一般に,過剰根充は作業長の測定ミスや根充操作時のガッタパーチャポイントの根尖孔外への突き出しが主な原因であるといわれている.根尖孔から2mm以内のわずかな過剰根充であれば根尖歯周組織に為害性はないが,それ以上の著しい過剰根充の場合は,術後の症状やX線写真によるチェックを行い注意深く対応する必要がある.本報告では,X線写真上で2.5?3.5mmのポイントの突出が認められた3症例(症例1:上顎第二大臼歯,3.0mm,症例2:下顎第一大臼歯,2.5mm,症例3:下顎第一小臼歯,3.5mm)について,最長6?14年の経過観察を行った結果を提示した.症例1では,突出ポイントは1年5カ月後に切断され3年8カ月後に消失し,6年8カ月後に根尖透過像も改善した.症例2では,突出ポイントは3年後に縮小傾向を示し,14年7カ月後には消失しており,根尖透過像の消失,歯槽硬線の明瞭化が観察された.症例3では,突出ポイントは13年6カ月後にわずかな部分を残して,ほとんどが吸収しており,同時に根尖透過像も消失していた.臨床症状は,症例1で打診痛が長期残存したほかには特記事項はほとんど認められなかった.当該歯は現在も健常に機能している.今回の症例は,術前診断,術中術後の情報を詳細に提示しながら過剰根充されたガッタパーチャポイントの経過と根尖病巣の治癒経過を長期間追跡したものであり,過剰根充の転帰を知るうえで興味深い臨床報告である. The Japanese Society of Conservative Dentistry 特定非営利活動法人日本歯科保存学会 The Japanese Journal of Conservative Dentistry 日本歯科保存学雑誌 0387-2343 51 5 485 492 20081031 10.11471/shikahozon.51.485 read0017481/published_papers/39305931 Hiroshima Yuka 廣島 佑香 Bando Mika 板東 美香 Kido Jun-ichi 木戸 淳一 Kataoka Masatoshi 片岡 正俊 Nagata Toshihiko 永田 俊彦 Sho-saiko-to increases the expression of calprotectin in human oral keratinocytes 小柴胡湯はヒト口腔上皮細胞のカルプロテクチン発現を増加する JAPANESE SOCIETY OF PERIODONTOLOGY 特定非営利活動法人 日本歯周病学会 Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology 特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集 2008 29 29 20080000 10.14833/amjsp.2008s.0.29.0 read0017481/published_papers/39304706 Ninomiya Masami 二宮 雅美 Yoneda Satoshi 米田 哲 Tanaka Hiroaki 田中 宏明 Seto Hiroyuki 瀬戸 浩行 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 Phase II Clinical Trial for Periodontal Regeneration by KCB-1D(FGF-2) -A Case Report of KCB-1D(FGF-2)-induced Periodontal Regeneration which was Verified by the Reentered Procedure- KCB-1D(FGF-2)歯周組織再生試験(後期第II相)ー治験終了後のリエントリーによって歯周組織再生を確認した一症例ー JAPANESE SOCIETY OF PERIODONTOLOGY 特定非営利活動法人 日本歯周病学会 Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology 特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集 2008 84 84 20080000 10.14833/amjsp.2008s.0.84.0 read0017481/published_papers/26440900 Mika Bando Yuka Hiroshima Masatoshi Kataoka Yasuo Shinohara Mark C. Herzberg Karen F. Ross Toshihiko Nagata Jun-Ichi Kido Interleukin-1 alpha regulates antimicrobial peptide expression in human keratinocytes IMMUNOLOGY AND CELL BIOLOGY 0818-9641 85 7 532 537 20071000 10.1038/sj.icb.7100078 17549071 read0017481/published_papers/26440897 Yuka Hiroshima Mika Bandou Masatoshi Kataoka Toshihiko Nagata Jun-ichi Kido Regulation of calprotectin expression in human keratinocytes in vitro Calprotectin, a heterodimeric complex of S100A8 and S100A9, is an antimicrobial peptide (AMP) expressed in healthy or inflamed epithelium and epidermis. The expression of calprotectin and other AMPs in keratinocytes is regulated by proinflammatory cytokines, bacterial products and keratinocyte differentiation modulators. Interleukin-1α (IL-1α), an autonomous cytokine in keratinocytes, induces keratinocyte differentiation and up-regulates calprotectin expression. Since keratinocyte differentiation is also specified&lt;KG&gt;by interactions with the basement membrane and connective tissue proteins, we sought to learn whether calprotectin expression is affected by extracellular matrix (ECM) proteins in the presence or absence of IL-1α. Human immortalized epidermal keratinocytes (HaCaT cells) were grown in vitro on different ECM substrates including type I collagen (Col I), type IV collagen (Col IV), fibronectin (FN) and laminin (LM) with or without IL-1α and were then cultured in basement membrane extract (Matrigel)-coated dishes or on a feeder layer of fibroblasts. Calprotectin expression in the cultured cells was investigated using immunohistochemical and northern blot analyses, and an enzyme-linked immunosorbent assay. The four ECM proteins (Col I, Col IV, FN and LM) had little effect on calprotectin expression. IL-1α significantly increased the expression of S100A8/ S100A9 mRNAs and calprotecin protein in the cells cultured on each of the ECM(Col I, Col IV, FN and LM)-coated dishes,but these ECM protein substrates did not show a further effect on calprotectin expression in the presence of IL-1α. On the other hand, Matrigel and the fibroblast-feeder layer slightly decreased the expression of S100A8/S100A9 mRNAs in HaCaT cells. These results suggest that calprotectin expression in keratinocytes is regulated by cytokines during epithelial-mesenchymal interactions.&lt;KG&gt;Nihon Shishubyo Gakkai Kaishi (J Jpn Periodontol) 49 : 224-232,2007. カルプロテクチンはS100A8とS100A9の2つの蛋白複合体から構成される抗菌ペプチドであり，上皮において発現している．カルプロテクチン発現は，炎症性サイトカイン，細菌産物や上皮細胞分化調節因子により調節されている．上皮細胞で恒常的に産生されるインターロイキン-1α( IL-1α) は上皮細胞分化を誘導し，カルプロテクチン発現を上昇させる．上皮細胞の分化は基底膜や結合組織の相互作用により制御されている．本研究では，細胞外基質(ECM)蛋白やIL-1αによるカルプロテクチン発現について検討した．ヒト上皮細胞株HaCaTをECM蛋白(I型とIV型コラーゲン，フィブロネクチン，ラミニン)コートディッシュに播種し，IL-1αの存在，非存在下で培養した．また，基底膜抽出物(マトリゲル)コートディッシュや線維芽細胞フィーダー層でも培養した．カルプロテクチンの発現は，免疫組織染色，ノーザンブロット法およびELISA法により分析した．その結果，4種のECM蛋白ディッシュで培養を行った場合，カルプロテクチン発現に変化は認められなかった．一方，IL-1αは培養ディッシュに関わらずカルプロテクチン発現を有意に上昇させたが，ECM蛋白はこの効果に影響を及ぼさなかった．また，マトリゲルや線維芽細胞フィーダー層ではカルプロテクチン発現の軽度の減少が認められた．これらの結果より，上皮細胞でのカルプロテクチン発現は上皮-間葉系においてサイトカインにより調節されていることが示唆された． JAPANESE SOCIETY OF PERIODONTOLOGY Journal of the Japanese Association of Periodontology 0385-0110 49 3 224 232 20070928 10.2329/perio.49.224 read0017481/published_papers/26977899 K. Yoshida H. Shinohara Suryono T. Haneji T. Nagata Arachidonic acid inhibits osteoblast differentiation through cytosolic phospholipase A(2)-dependent pathway ORAL DISEASES 1354-523X 13 1 32 39 20070100 10.1111/j.1601-0825.2006.01239.x 17241427 read0017481/published_papers/26743559 M Ogino J Kido M Bando N Hayashi C Wada T Nagata F Nishimura Y Soga S Takashiba T Kubota M Itagaki Y Shimada H Tai H Yoshie N Yamazaki Y Shinohara M Kataoka alpha 2 integrin+807 polymorphism in drug-induced gingival overgrowth JOURNAL OF DENTAL RESEARCH 0022-0345 84 12 1183 1186 20051200 10.1177/154405910508401217 16304451 read0017481/published_papers/25673830 辻 けい子 堀内 信也 二宮 雅美 永田 俊彦 森山 啓司 骨粗鬆症を有する両側性口唇口蓋裂患者に対し歯周・矯正治療を並行して行った1症例 (公社)日本矯正歯科学会 日本矯正歯科学会大会プログラム・抄録集 63 302 302 20041100 read0017481/published_papers/26437590 Masami Ninomiya 二宮 雅美 Nobuyuki Kamata 鎌田 伸之 Masaru Nagayama 長山 勝 Toshihiko Nagata 永田 俊彦 エナメル基質タンパクの歯の移植への応用 the Quintessence ザ・クインテッセンス 23 7 89 96 20040000 read0017481/published_papers/35102670 ASANO Masahiro 浅野 将宏 ASAHARA Yoji 淺原 洋士 KIRINO Akinori 桐野 晃教 OHISHI Mika 大石 美佳 AKIMARU Noriko 明丸 倫子 HAMA Hideki 濱 秀樹 SURYONO スルヨノ SHIONOYA Akemi 塩野谷 明美 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Case Report of an Early-onset Periodontitis Patient Showing Self-Arrest of Alveolar Bone Loss after Puberty 思春期後に歯槽骨吸収が自然停止した早期発症型歯周炎患者の1症例 Early-onset periodontitis (aggressive periodontitis) is characterized by rapid attachment loss and alveo-lar bone loss, despite a healthy systemic condition. The severity of this disease is closely associated with both highly virulent pathogens and highly susceptible subjects. Although the tissue destruction takes place very rapidly, the progression of attachment loss and bone loss may be self-arresting in some patients. This case report describes the 18-year clinical history of an early-onset periodontitis patient who did not receive continuous treatment but who experienced the self-arrest of alveolar bone loss after puberty. The patient first visited the Orthodontic Department of Tokushima University Dental Hospital complaining of maloc-culusion when she was 11 years old. Clinical findings at that time showed moderate gingivitis with no alveolar bone loss. She refused orthodontic therapy because of the need to undergo tooth extraction prior to the therapy. She returned to our hospital when she was 17 years old, complaining of a pain in her lower left first molar. This time, severe generalized periodontitis was found, and a radiographic examination revealed marked alveolar bone loss in several teeth, including the lower left first molar. While local irrigation and medication relieved the pain, she visited our hospital once again at the age of 21 years, complaining of the same symptoms in the same molar. The lower left first molar was extracted at this time. When the patient was 29 years old, she returned to our hospital once again because of pulpitis of her upper molar. Although gingival inflammation and deep periodontal pockets were still present, the degree of alveolar bone loss had not changed since the examinations that were performed when she was 17 and 21 years old. These data indicate that periodontal destruction progressed while she was a teen-ager and then arrested, despite the absence of cause-related therapy. These results suggest that the progression of attach-ment and bone loss may be self-arresting in some patients. J Jpn Soc Periodontol, 45 : 279∼288, 2003. 早期発症型歯周炎(侵襲性歯周炎)は, 全身的に健康であるにもかかわらず, 口腔内では比較的短期間に急激な歯周組織の破壊が起こることを特徴とする歯周炎である．疾患の重症度はビルレンスの強い細菌の関与や高い疾患感受性をもつ個人体質に依存していると考えられている．病状の進行に関しては, 若年期に急激な歯周組織の破壊が起こり, その後自然に停止する場合があると言われている．本症例報告では, 継続的な歯周治療が患者の都合によりほとんど施されなかった早期発症型歯周炎の一例について, 思春期前から18年間の臨床データを紹介する．患者は初診時11歳の女性．上顎前歯部の叢生を主訴に徳島大学歯学部附属病院矯正科を受診した．当時, 中程度の歯肉炎が認められたものの, 歯槽骨吸収像は観察されなかった．このとき患者は矯正治療の前段階として永久歯の抜歯が必要であると指摘されたため歯科矯正処置を断念した．6年後(17歳)に下顎左側第一大臼歯の疼痛を主訴に再来院した際には, 全顎にわたって重度の歯周炎が発症しており, 患歯をはじめ多数の部位に著しい歯槽骨吸収が生じていた．さらに, 21歳のときに再び下顎左側第一大臼歯に同様の症状を訴えて救急来院し, 同歯は抜歯に至った．その後, 治療は再び中断したが, 初診から18年後(29歳)に上顎大臼歯歯髄炎のため来院した際には, 歯肉の炎症や歯周ポケットが依然として残存していたものの, 歯槽骨吸収のさらなる進行はほとんど認められなかった．以上のように, この患者では思春期(10歳代)に急激な歯周組織の破壊が起こり, 思春期以後(17歳以降)に継続的な歯周基本治療を行っていないにもかかわらず, 歯周炎が大きく進行しなかったことが明らかとなった．本症例は従来言われていた「早期発症型歯周炎は自然に停止する場合がある」ことを示した興味ある報告と言える． JAPANESE SOCIETY OF PERIODONTOLOGY JOURNAL OF THE JAPANESE ORGANISATION FOR RESEARCH OF PERIODONTOLOGY 日本歯周病学会会誌 0385-0110 45 3 279 288 20030928 10.2329/perio.45.279 read0017481/published_papers/26437592 Masami Ninomiya 二宮 雅美 Akane Takemura 武村 あかね Toshihiko Nagata 永田 俊彦 MMP 阻害剤の歯槽骨吸収抑制作用 the Quintessence ザ・クインテッセンス 22 9 41 47 20030000 read0017481/published_papers/38708482 Nakamura Teruo 中村 輝夫 Suryono スルヨノ Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 Quantitative Analyses of Propeptide of Type I Procollagen and Osteocalcin in Gingival Crevicular Fluid of Periodontal Disease Patients 歯周炎患者の歯肉溝滲出液中に含まれるI型プロコラーゲンペプチドおよびオステオカルシンの定量分析 Biochemical markers in gingival crevicular fluid (GCF) have been studied to precisely and objectively diagnose periodontal disease. Carboxy terminal propeptide of type I procollagen (P I CP) and osteocalcin (OCN) are bone-related proteins produced by osteoblasts that are useful clinical indicators of metabolic bone diseases such as osteoporosis and hyperparathyroidism. To determine bone-related markers in GCF, we studied the presence of P I CP and OCN in GCF from periodontitis patients using immunoen-zymatic kits and the relationship between P I CP/OCN amounts and clinical parameters involving gingival index (GD, probing depth (PD) , and bleeding on probing (BOP). GCF samples were collected with paperstrips from 70 diseased sites (PD>4 mm) in 70 periodontitis patients and from 30 healthy sites (PD< 3 mm) in 30 healthy volunteers. A mean of 1.04 ng/site (0.91 ng/μl) P I CP was found at diseased sites and a mean of O . 17 ng/site (0.38 nglpl) at healthy sites, showing a 6 . 1-fold amount of P 1 CP at diseased sites. A mean of 56 .3 pg/site (47.6 pg/μl) OCN was found at diseased sites and a mean of 38.8 pg/site (1203.9 pg/μl) at healthy sites, showing a 1 .5-fold amount of OCN at diseased, sites. Both P 1 CP and OCN in GCF correlated positively with GI and PD, especially P 1 CP, P 1 CP greatly increased at BOP-positive sites (5.5-fold) , suggesting that P I CP may reflect disease activity in alveolar bone metabolism. These results indicate that P 1 C,P and OCN are present in GCF and that these molecules may be useful as a bone-related marker in periodontitis. P 1 CP may prove to be an especially potent marker for bone turnover in periodontitis. J Jpn Soc Periodontol, 44 : 73-81, 2002. 歯周病を正確かつ客観的に診断するために,歯肉溝滲出液(GCF)中の生化学的な指標を見出そうという研究が長年行われている．I型プロコラーゲンC末端ペプチド(P1CP)およぴオステオカルシン(OCN)は,骨芽細胞によって産生される骨代謝関連蛋白であり,骨粗髷症や副甲状腺機能亢進症のような代謝性骨疾患において有用な臨床指標として用いられている．本研究では,GCF中の骨代謝関連指標を探索するために,PICPとOCNがGCF中に存在するかどうかを酵素抗体法キットを用いて検証するとともに,P1CP童およびOCN量を定量し,それらの値と臨床パラメーターである歯肉炎指数(GI),プロービング値(PD),プロービング時の出血(BOP)の有無との関連をそれぞれ調べた．GCF試料はぺ一パーストリップスを用いて,70名の歯周炎患者から歯周ポケット70部位(PD>4mm),およぴ30名の健常者から健常歯肉溝30部位(PD<3mm)を選んで採取された．GCF中のP1CP量はポケット部位で平均1.04ng/site(0.91ng/μl),健常部位で0.17ng/site(0/38ng/μl)であり,ポケット部位で6.1倍のP1CP量が探知された．OCN量はポケット部位で平均56.3pg/site(47.6pg/μl),健常部位で38.8pg/site(123.9pg/μl)であり,ポケット部位で1.5倍のOCN量が探知された．PICP量およぴOCN量は,いずれもGIおよぴPDと正の相関関係を示した．とくに,P1CPは両方の指標と強く相関していた．さらに,P1CP量はBOPの認められる部位で高く(5.5倍値),歯槽骨代謝における疾患活動性を反映している可能性が示唆された．以上の結果は,GCF中にはP1CPおよびOCNが存在し,これらの分子は歯周炎の骨代謝関連指標になりうる可能性があることを示すものであり,とくにPICPは歯周炎における骨代謝回転を反映する指標として有カであることが示唆された． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 44 1 73 81 20020328 10.2329/perio.44.73 read0017481/published_papers/38708497 Kido Jun-ichi 木戸 淳一 Kataoka Masatoshi 片岡 正俊 Ohishi Keiji 大石 慶二 Asahara Yoji 淺原 洋士 Nagata Toshihiko 永田 俊彦 A Case Report on Gingival Overgrowth in a Patient Receiving Manidipine Hydrochloride 塩酸マンジピン服用患者にみられた歯肉増殖症の1例 Gingival overgrowth is a frequent side effect of nifedipine therapy in patients suffering from cardiovas-cular diseases such as hypertension and angina pectoris. Nifedipine is a calcium channel blocker, and other drugs having the similar action, e.g., verapamil and diltiazem, are also reported to induce gingival over-growth. We report a clinical case of gingival overgrowth in a 69-year-old man receiving manidipine hydrochloride, a calcium channel blocker. The overgrown gingiva was observed in most of the teeth and was marked in the lower anterior and lower right posterior teeth. As chronic periodontitis was complicated by overgrowth, initial therapy was thorough. Medication was changed from manidipine chloride to angiotensin converting enzyme inhibitor. After initial therapy and subsequent periodontal surgery, over-grown gingiva disappeared and the probing depth improved in all teeth (< 3 mm). No recurrence was observed in maintenance therapy. From this case, we confirmed the importance of initial therapy and the necessity of periodontal surgery vvhen gingival overgrowth appears in chronic periodontitis. It was also suggested that the number of patient with gingival overgrowth induced by calcium channel blockers other than nifedipine will increase. J. Jpn. Soc. Periodontol., 43 : 388-395, 2001. 高血圧症および狭心症治療薬ニフェジピンを服用している患者の一部に歯肉増殖症が発現することが知られている．ニフェジピンはカルシウム拮抗作用を有し,他のカル・シウム拮抗剤(ベラパミル,ジルチアゼムなど)でも同様の副作用が発現すると言われている．本症例報告ではカルシウム拮抗剤・塩酸マニジピンを服用している69歳男性にみられた歯肉増殖症の症例について紹介する．増殖歯肉は全顎的に認められ,下顎前歯部と下顎右側臼歯部で著明であった．この症例は成人性歯周炎を伴った歯肉増殖症であることから,徹底的な歯周基本治療を行い,同時に服用薬剤をアンギオテンシン変換酵素阻害剤に変更した．さらに歯周外科治療の結果,増殖歯肉は除去されプロービング値もすべて3mm以内に改善した．現在,再発も認められずメインテナンスを行っている．本症例を通じて,成人性歯周炎を伴う歯肉増殖症における歯周基本治療の重要性と歯周外科治療の必要性を確認するとともに,今後ニフェジピン以外のカルシウム拮抗剤で歯肉増殖症を起こす患者が増加する可能性が示唆された． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 43 4 388 395 20011228 10.2329/perio.43.388 read0017481/published_papers/38706942 Ohba Takanobu 大場 堂信 Akazawa Kayoko 赤沢 佳代子 Ninomiya Yohsuke 二宮 洋介 Kirino Akinori 桐野 晃教 Akimaru Noriko 明丸 倫子 Ishimoto Tomoko 石本 智子 Tono Sayuri 戸野 早由利 Nakamura Teruo 中村 輝夫 Kataoka Masatoshi 片岡 正俊 Shinohara Hiroyuki 篠原 啓之 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 Epidemiologic Study of Periodontal Conditions in Patients Undergoing Renal Dialysis 人工透析患者の歯周病罹患度に関する疫学的研究 Some 180 , OOO patients are currently undergoing renal dialysis in Japan. Dialysis does not, however, compensate for all kidney functions, e.g., erythropoietin production and vitamin D3 activation, which are very important reactions in the human body, are both inhibited. Osteoporotic disorder resulting from these functional deficiencies is a prominent side effect in renal dialysis patients. We suspect chronic renal failure to be a risk factor in periodontal disease. To determine the relationship between periodontitis and renal failure, we studied periodontal conditions in 38 subjects undergoing renal dialysis, together with a control group of 42 similar-aged healthy subjects. Community periodontal index of treatment needs (CPITN) assessment showed that dialysis patients, at 2 . 4±0. 1, scored higher than controls, at 1 . 9±0. 1 (p < 0 . 05) . No difference was seen between groups in the simplified calculus index (CI-S) score. The number of missing teeth, at 6 . 1 ± 1 . 3, was 2 . 2 times greater in dialysis patients than in controls, at 2 . 8±l 0 . 8 (p < 0 . 05) . When patients were divided into 4 groups by dialysis duration, no significant difference was observed in any index. No correlation was seen between blood concentration of parathyroid hormone (PTH) and alveolar bone height or between PTH and CPITN. Of the 38 dialysis patients, 7 suffered from diabetic nephropathy, and most suffered from severe periodontitis. Compared to the other 31 patients, those having diabetic nephropathy showed an increase in missing teeth, from 3 . 9±1 1 . 1 to 15.9±3.6 (p < 0 . 05) , and a decrease in alveolar bone height from 79.1% to 58.6% (p < 0.05) . When the 31 nondiabetic dialysis patients were compared to controls, these patients, at 2 . 3±0. 1, scored a higher CPITN than controls, at 1.9±0.1(p < 0. 05), indicating that nondiabetic patients suffered more from periodontitis than healthy subjects. This suggests that patients with chronic renal failure undergoing dialysis are at risk for periodontitis and that those with diabetic nephropathy suffer more from severe periodontitis. J. Jpn. Soc. Periodontol., 42 : 307-313, 2000. わが国で腎不全により人工透析を受けている患考は現在約18万人いると言われている．透析処置では腎臓のすべての機能を補うことはできず,例えばエリスロポエチンの産生やビタミンD_3の活性化といった生体にとって重要な反応が行われなくなる．これらの腎機能障害に由来した骨病変は透析患者にみられる主要な副作用の一つである．著者らは人工透析処置を受けている慢性腎不全患者は歯周病に対する感受性の高い集団ではないかと考え,その関連を追求するために透析患者38名の歯周組繊診査を行った．対照群として同年代の健常者42名を選び,同様の歯周組繊診査を行った．CPITN(歯周治療必要度指数)を調べた結果,透析患者群は対照群より高い値を示した(2.4±0.1 vs. l.9±0.1;p < 0.05)．CIS(簡略化歯石指数)では,2群間に有意差は認められなかった．欠損歯数では,透析群の方が2.2倍多かった(6.1±1.3 vs. 2.8±0.8;p < 0.05)．次に,透析期間の違いによって患者を4グループに分けて分析したところ,指標値に差は認められなかった．また,透析患者の血中PTH(副甲状腺ホルモン)濃度と歯槽骨レベルならびにCPITNとの相関を調べたが,有意な相関は見い出せなかった．一方,透析患者38名のうち7名が糖尿病由来で人二透析に至った患者(糖尿病性腎症)であり,これらの患者のほとんどに重度の歯周炎が認められ,残り31名の透析患者と比較すると,欠損歯数の増加(15.9±3.6 vs. 3.9±l.1;p < 0.05)および歯槽骨レベル(%)の低下(58±6 vs. 79±1; p < 0.05)が認められた．さらに,糖尿病性腎症以外の透析患者31名と対照群とを比較した場合,CPITNにおいて有意差が認められ(2.3±0.1 vs.1.9±0.l:p < 0.05),糖尿病性腎症を除いた透析患者においても健常者より歯周病罹患度が高いことが示された．以上の結果から,人工透析処置を受けている慢性腎不全患者の歯周病罹息度は健常人より高く,慢性腎不全が歯周病のリスクファクターになりうる可能性が示唆されるとともに,人工透析処置を受けている糖尿病性腎症患者はそれ以外の疾患由来の患者よりも童度の歯周病を有する傾向が強いことが示された． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 42 4 307 313 20001228 10.2329/perio.42.307 read0017481/published_papers/26437595 Masami Ninomiya 二宮 雅美 大場 堂信 二宮 洋介 中野 恵子 Mika Oishi 大石 美佳 浅原 洋士 Masatoshi Kataoka 片岡 正俊 篠原 啓之 Jun-ichi Kido 木戸 淳一 Toshihiko Nagata 永田 俊彦 ハムスター実験的歯周炎におけるプロスタグランディンE2の歯槽骨吸収抑制効果 The Japanese Society of Conservative Dentistry 日本歯科保存学会雑誌 43 6 1138 1144 20000000 read0017481/published_papers/38798844 NISHIKAWA Seiji 西川 聖二 NAGATA Toshihiko 永田 俊彦 SUMITANI Koji 住谷 光治 KIDO Jun-ichi 木戸 淳一 ISHIDA Hiroshi 石田 浩 Report of a Case of Severe Gingivitis Associated with Mouth-breathing-Long-term Follow-up 口呼吸が関連した歯肉炎の長期観察症例 We present a clinical case of severe gingivitis associated with mouth-breathing followed up for about 10 years. Oral examination showed that protrusion of both upper incisors made it impossible for the patient's lips to cover the upper anterior teeth at rest, suggesting that mouth-breathing was the result of the lack of lip seal. The gingiva was hyperplastic mainly on the upper anterior segment, where the anatomical crowns were partially covered by the enlarged gingiva. The plaque control record (PCR) was 80%, indicating poor oral hygiene. Gingivectomy was performed after instruction to brush the teeth, but the PCR remained high (40%). Thereafter, treatment was discontinued for the patient's own reasons. Five years later the patient was again referred for evaluation of her gingival condition. The gingiva was erythematous, enlarged and bled easily when probed. Extensive tooth brushing instructions, and scaling and root planing for 1.5 years resulted in satisfactory progress. After initial preparation, minor tooth movement using a sectional wire was performed to reduce protrusion of the upper incisors. Myofunction therapy in addition to tooth movement permitted easier nasal breathing. In spite of unsatisfactory plaque control, no signs fo recurrence of the severe gingivitis have been observed during a 2.5-year follow-up period. These findings suggest that mouth-breathing is a factor in aggravating preexisting gingivitis, but not in causing gingivitis. 口呼吸が関連した歯肉炎の長期観察症例を報告した．初診時の診査から口呼吸の原因が[numerical formula]の唇側への突出による口唇閉鎖困難であることが示唆された．歯肉は線維性に増殖し,上顎前歯部で顕著であった．口腔清掃状態は不良で,プラークコントロールレコード(PCR)は80%であった．ブラッシング指導に引き続いて歯肉切除を行なったが,その後患者の都合によって治療が中断した．5年後の再来院時も口腔清掃状態は不良で,歯肉は発赤を伴い増殖し,歯肉からの出血,排膿も著明であった．約1年半の徹底した初期治療によって歯肉はほぼ正常レベルまで改善された．その後,[numerical formula]の矯正移動によって口呼吸癖が改善され,メインテナンスに移行した．メインテナンス中,口腔清掃が不良な期間があったにもかかわらず,症状の再発は観察されなかった．以上の治療経過から,口呼吸は歯肉炎の発症因子ではなく,存在する歯肉炎の増悪因子であることが示唆された． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 37 1 175 184 19950328 10.2329/perio.37.175 read0017481/published_papers/26437598 Masami Ninomiya 二宮 雅美 Takanobu Ohba 大場 堂信 Yohsuke Ninomiya 二宮 洋介 Keikko Nakano 中野 恵子 Mika Oishi 大石 美佳 Yoji Asahara 淺原 洋士 Masatoshi Kataoka 片岡 正俊 Hiroyuki Shinohara 篠原 啓之 Jun-ichi Kido 木戸 淳一 Toshihiko Nagata 永田 俊彦 Inhibitory Effects of Prostaglandin E2 on Alveolar Bone Resorption in Hamster Experimental Periodontitis ハムスター実験的歯周炎におけるプロスタグランディンE2の歯槽骨吸収抑制効果 The Japanese Journal of Conservative Dentistry 日本歯科保存学雑誌 0387-2343 43 6 1138 1144 19881200 read0017481/published_papers/38798742 SHINOHARA Hiroyuki 篠原 啓之 NISHIKAWA Seiji 西川 聖二 YOSHIMURA Akihiko 吉村 明彦 KASAHARA Shinji 笠原 信治 KIDO Jun-ichi 木戸 淳一 TSUTSUI Hideshi 筒井 英士 HAMASAKI Akihiro 浜崎 章弘 KOHDA Naohiko 幸田 直彦 NAGATA Toshihiko 永田 俊彦 ISHIDA Hiroshi 石田 浩 WAKANO Yoichi 若野 洋一 Studies on Polyamine of Gingival Tissues in Marginal Periodontitis 歯周疾患患者の歯肉ポリアミン量について Polyamine contents of gingival tissues from thirteen patients of marginal periodontitis were examined. According to clinical estimations after the initial preparation, gingival tissues were divided into two groups, inflammatory and normal gingiva. Polyamine extracted from the tissues were dansylated and separated by silica gel thin-layer chromatography. Putrescine content in the inflammatory gingiva was 0.80±0.14nmol/mg protein, which was about twice that in the normal gingiva. No significant differences in the contents of spermidine and spermine were observed between inflammatory and normal tissues. These findings suggest that putrescine is related to the regulation of inflammatory process and recovery in periodontal disease. 歯周疾患に罹患した患者から,臨床的に炎症歯肉,健常歯肉と診断された歯肉組織片を採取し,含まれる各ポリアミン量をシリカゲル薄層クロマトグラフィーを用いて分離し,定量した．炎症歯肉組織のスペルミジン,スペルミン量は健常歯肉のそれと比較して有意な差は認められなかったが,プトレッシン量は炎症歯肉で約2倍に上昇していた． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 30 1 142 147 19880328 10.2329/perio.30.142 read0017481/published_papers/38162142 KASAHARA Shinji 笠原 信治 ISHIDA Hroshi 石田 浩 NAGATA Toshihiko 永田 俊彦 KIDO Jun-ichi 木戸 淳一 SHINOHARA Hiroyuki 篠原 啓之 TANIGUCHI Norio 谷口 典生 KAWAHITO Takako 川人 貴子 II Hiroshi 伊井 廣 WAKANO Yoichi 若野 洋一 γ-Carboxyglutamic Acid in Rat Alveolar Bone 歯槽骨オステオカルシンに関する研究 : 1.ラット下顎骨のγ-カルボキシグルタミン酸量について Distribution of γ-carboxyglutamic acid (Gla) in rat mandibula was examined using high-performance liquid chromatography. Rat mandibula was divided into three portions:incisor, molar and ram us. Gla could be detected in all bone samples. Both in 4 and 8 weeks-old rat, high Gla content was found in incisor-portion as compared with other two portions (1.7-2.8 fold). There was no difference in Gla contents between molar- and ramus-portions. Since the incisal alveolar bone has been indicated to show an active bone metabolism, the distribution of Gla content in this system may reflect the characteristics of alveolar bone remodeling. 歯槽骨の吸収, 形成などの骨改造におけるオステオカルシンの動態を追求するため, ラット下顎骨 (切歯部歯槽骨, 臼歯部歯槽骨, 下顎枝) のγ-カルボキシグルタミン酸 (Gla) について, 高速液体クロマトグラフィー法を用いて検出, 定量した．骨代謝が盛んであると考えられる切歯部歯槽骨は, 臼歯部歯糖骨や下顎枝と比較して有意に多く Gla を含有していたことより, 本系における Gla は歯槽骨骨代謝活性の一指標になりうるものと考えられる． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 29 2 538 544 19870628 10.2329/perio.29.538 read0017481/published_papers/38705983 NAGATA Toshihiko 永田 俊彦 ISHIDA Hiroshi 石田 浩 MASUDA Tadayuki 益田 忠幸 KASAHARA Shinji 笠原 信治 SHINOHARA Hiroyuki 篠原 啓之 KIDO Jun-ichi 木戸 淳一 WAKANO Yoichi 若野 洋一 Studies on Gingival Hyperplasia Induced by Diphenylhydantoin : Effects of Diphenylhydantoin on DNA, RNA, and Protein Synthesis in Cultured Bovine Gingival Explants Diphenylhydantoin (DPH)による歯肉増殖症に関する研究 : ウシ歯肉組織培養系におけるDPHの核酸および蛋白合成能に及ぼす影響 The effects of diphenylhydantion (DPH) on DNA, RNA, and protein synthesis in cultured bovine gingival explants were investigated. Explants were supported by siliconized lens paper at the surface of chemically defined synthetic medium (M-199). DPH, at a concentration of 5μg/ml of medium, caused increase of DNA synthesis in three cases of six separate experiments. Furthermore insulin and dexamethasone significantly augmented the stimulatory effect of DPH on DNA synthesis. These three cases in which DNA synthesis were enhanced were named " Responder group " , and other three cases " Non-Responder group " . In " Responder group " RNA synthesis was also stimulated by DPH, but not in " Non-Responder group " . Protein synthesis was not affected by DPH in either group. These results suggest that DPH is involved in the regulation of cell proliferation in cultured gingival explants. ウシ歯肉の組織培養を行い, Diphenylhydantoin(DPH)の核酸およぴ蛋白合成能に及ぼす影響を検索したところ, 実験した6例のうち3例にDPHによるDNA合成能の促進が認められ, 他の3例は変化が認められなかった．前者を応答群, 後者を非応答群とすると, 応答群におけるDPHの至適濃度は5μg/mlであった．RNA合成能は, 応答群ではDPHにより促進されたが, 非応答群では変化がなかった．蛋白合成能は, 応答群も非応答群もDPHの影響が認められなかった． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 28 1 161 167 19860328 10.2329/perio.28.161 read0017481/misc/39778405 小林 哲夫 木戸 淳一 石原 裕一 大森 一弘 三谷 章雄 高柴 正悟 永田 俊彦 吉江 弘正 歯周炎と全身疾患(糖尿病・関節リウマチ)に共通するリスク遺伝子の解析 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 59 128 128 20170400 read0017481/misc/26437606 Masami Ninomiya 二宮 雅美 Toshihiko Nagata 永田 俊彦 全顎的な歯肉のびらん・接触痛 DENTAL DIAMOND デンタルダイヤモンド 0386-2305 41 13 19 20 20161000 read0017481/misc/26437605 Toshihiko Nagata Masami Ninomiya Chie Mihara-Wada Jun-ichi Kido Seiji Nishikawa Etiology of drug-induced gingival overgrowth Current Issues in Periodontics 1 8 20161000 read0017481/misc/35026284 LEW Jung Hwan 成石浩司 梶浦由加里 西川泰史 木戸淳一 永田俊彦 カロテノイドは高グルコース下で培養したヒト歯肉線維芽細胞のIL-6誘導体蛋白分解酵素産生を抑制する 日本歯周病学会会誌(Web) 1880-408X 58 null null 20160000 read0017481/misc/26437607 Masami Ninomiya 二宮 雅美 Toshihiko Nagata 永田 俊彦 下顎前歯部の動揺 DENTAL DIAMOND デンタルダイヤモンド 0386-2305 40 15 21 22 20151100 read0017481/misc/26440903 Jun-ichi Kido 木戸 淳一 Mika Bandou 板東 美香 E Sakamoto Yukari Kajiura 梶浦 由加里 Toshihiko Nagata 永田 俊彦 Effects of advanced glycation end-products on pathogenesis of diabetes-associated periodontitis 糖尿病関連歯周炎の病態に及ぼす最終糖化産物の影響 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 56 1 17 24 20140000 10.2329/perio.56.17 read0017481/misc/26437608 Masami Ninomiya 二宮 雅美 Toshihiko Nagata 永田 俊彦 下顎前歯部の歯肉腫脹 DENTAL DIAMOND デンタルダイヤモンド 0386-2305 38 8 133 134 20130601 read0017481/misc/26437609 Toshihiko Nagata 永田 俊彦 Masami Ninomiya 二宮 雅美 2.各論 1)歯周病 (特集 透析患者の口腔衛生) 透析フロンティア 21 2 12 16 20110500 read0017481/misc/37626715 MIHARA WADA Chie 美原 和田 智恵 SETO Hiroyuki 瀬戸 浩行 HORIBE Masumi 堀部 ますみ KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Histological Analysis of Alveolar Bone in Cyclosporin A-treated Rats サイクロスポリンA投与ラットにおける歯槽骨の組織学的解析 Cyclosporin A (CsA) is used widely as an immunosuppressive agent. CsA therapy induces several side effects such as nephrotoxicity, hepatoxicity, gingival overgrowth, and osteoporosis. Investigations of CsA-induced osteoporosis have been reported from clinical and basic aspects. Osteoporosis is recently thought to be one of the risk factors of periodontal disease, which is characterized by periodontopathic bacteria infection and alveolar bone loss. In this study, we investigated morphological changes of alveolar bone in CsA-treated rats. Fifteen-day-old rats were fed a powered diet containing or lacking CsA (50-200 mg/kg) for 8-30 days. On days 8, 16, and 30, they were sacrificed. After taking out the mandiblae, histomorphometric analyses were performed to examine morphorogic changes in the alveolar bone. The alveolar bone around the mesial root of the first molar were analysed using micro CT. The distance from the cement-enamel junction (CEJ) to the lingual alveolar bone crest (ABC) and the construction of the alveolar bone proper were measured by micro CT. Decalcified semi thin sections were stained with tartarte-resistant acid phosphatase (TRAP), and then histomorphometrical measurement was performed. The distance from CEJ to ABC was similar between CsA-treated and control rats. Micro CT analysis revealed that the volume and thickness of the alveolar bone in CsA-treated rats were decreased on days 16 and 30 compared with the controls. The ratio of bone surface to bone volume in CsA-treated rats was significantly increased on days 16 and 30. There was no difference in the number of osteoblasts and osteoclasts on the bone surface during experimental periods in both CsA-treated and control rats. The present study suggests that CsA administration may be a risk factor for periodontal disease progression, because CsA induced a decrease in alveolar bone volume and thickness. サイクロスポリンA(CsA)は,免疫抑制剤として臓器移植患者や自己免疫疾患患者に広く用いられている.CsA服用の副作用として腎障害,肝障害,および歯肉増殖症が挙げられるが,これらの副作用に加えて最近,CsAによって骨粗鬆症が誘発されることが実験的にも臨床的にも報告されている.一方,骨粗鬆症を併発している歯周病患者では付着の喪失が大きく,骨粗鬆症は歯周病のリスクファクターの一つとして知られている.このことから,CsAは,歯周病において歯肉増殖症を誘発させるだけでなく,骨代謝にも影響を及ぼしていると考えられる.しかしながら,CsAの歯槽骨代謝に及ぼす影響については不明な点が多い.そこで本研究では,CsAがラット歯槽骨に及ぼす影響について,ラット実験モデルを用い,組織学的検索を行った.15日齢雄性Fischer系ラットを用い,実験群ではCsA(50-200mg/kg)を含む粉末飼料を,対照群では粉末飼料のみを食餌として与えた.飼育開始後8,16日および30日目に両群より下顎骨を採取し,CsAの下顎骨への影響を検討した.マイクロCT解析では,第一臼歯セメントエナメル境(CEJ)から歯槽骨骨頂(ABC)間の距離(CEJ-ABC)の計測,および周囲歯槽骨の三次元的な形態計測を行った.また,組織学的分析では同試料の脱灰薄切切片を作製し,酒石酸抵抗性酸性ホスファターゼ(TRAP)染色を行い,TRAP陽性破骨細胞数,さらに骨芽細胞数についても測定を行った.マイクロCT解析の結果,実験期間を通じて実験群と対照群でCEJ-ABC間距離に有意な差は認められなかった.CsA8日投与群では歯槽骨の形態に対照群と差は認められなかったが,16日および30日のCsA投与群では対照群と比較して有意に同部の骨密度および骨梁幅の減少が観察された.さらに,骨吸収の指標である骨の表面積/体積は実験群で有意な増加が認められた.一方,TRAP陽性破骨細胞数および骨芽細胞数は,飼育開始8,16日および30日のいずれも両群で差が認められなかった.これらのことから,CsAは単独では歯周炎を発症させないが,骨代謝に影響を及ぼしていることが確認できた.本研究の結果から,CsAは歯周炎における歯槽骨吸収に対して増悪因子として作用することが示唆された. The Japanese Society of Conservative Dentistry 特定非営利活動法人日本歯科保存学会 日本歯科保存学雑誌 0387-2343 51 3 246 255 20080630 read0017481/misc/38520466 NINOMIYA Masami 二宮 雅美 TANAKA Hiroaki 田中 宏明 NAGATA Toshihiko 永田 俊彦 Diagnostic Difficulty in the Case of Vertical Root Crack 診断困難な垂直性歯根破折症例 日本歯科保存学雑誌 0387-2343 51 165 165 20080507 read0017481/misc/38520153 INAGAKI Yuji 稲垣 裕司 OHBA Hirofumi 大場 博史 NAGATA Toshihiko 永田 俊彦 Mineralized Tissue Formation and Osteopontin Expression in Cultured Dental Pulp Cells Derived from Type 2-Diabetes Rat 2型糖尿病ラット由来歯髄細胞培養系における石灰化物形成とオステオポンチン産生 日本歯科保存学雑誌 0387-2343 51 138 138 20080507 read0017481/misc/25643551 MIHARA-WADA Chie 美原(和田) 智恵 SOGA Yoshihiko 曽我 賢彦 TAKASHIBA Shogo 高柴 正悟 NAGATA Toshihiko 永田 俊彦 KIDO Jun-ichi 木戸 淳一 BANDO Mika 板東 美香 KATAOKA Masatoshi 片岡 正俊 KUBOTA Takehiko 久保田 健彦 ITAGAKI Manami 板垣 真奈美 SHIMADA Yasuko 島田 靖子 TAI Hideaki 田井 秀明 YOSHIE Hiromasa 吉江 弘正 NISHIMURA Fusanori 西村 英紀 Examination of α2 Integrin Polymorphism in Drug-induced Gingival Overgrowth : α2 Integrin+1648 G/A Polymorphism 薬物誘発性歯肉増殖症の発症に関与する遺伝子多型の検索 : α2インテグリン+1648G/A遺伝子多型 Drug-induced gingival overgrowth is a side effect observed in patients taking calcium channel blockers, anti-epileptic drugs or immunosuppressant drugs. Some reports had showed different mechanisms for the incidence of gingival overgrowth, and we recently showed that gingival overgrowth was caused by collagen accumulation via drug-induced inhibition of α2 integrin expression and collagen phagocytosis in gingival fibroblasts. However, the reason for the difference in the incidence of gingival overgrowth in the reports was unclear. Single polymorphism (SNP) is known to be a hereditary factor associated with the incidence of some diseases. We showed that α2 integrin +807C/T polymorphism was correlated to the incidence of gingival overgrowth, but was not directly related to that induction because +807C/T polymorphism does not result in the change of amino acid sequence, suggesting that other SNP near the +807 site may cooperatively affect the incidence of gingival overgrowth. In the present study, we investigated the association between incidence of gingival overgrowth and α2 integrin +1648 G/A polymorphism, which is a linkage disequilibrium for +807C/T. The subjects were 98 patients who took calcium channel blockers, phenytoin or cyclosporin A. Gingival overgrowth was evaluated by the standard of McGaw et al.. Peripheral blood samples were collected from 45 subjects with gingival overgrowth (case group) and 53 subjects without it (control group). Genome DNA was extracted from blood and the α2 integrin +1648 site was analyzed by sequencing. The genotype distribution of the +1648 G/A polymorphism showed that GG genotype was highly present in 95.6% and 88.7% of the case and control groups, respectively, and GA genotype was 4.4% and 9.4%, respectively, and AA genotype occurred in only one subject in the control group. The frequency of the +1648 G allele was 97.8% and 94.7% in the case and control groups, respectively, and that of the +1648 A allele was 2.2% and 5.3%. Significant differences were not observed between the two groups. This result suggests that the incidence of drug-induced gingival overgrowth is not associated with human α2 integrin +1648 G/A polymorphism, but may be associated with a cooperation of +807C/T and other than + 1648 G/A polymorphism. 薬物誘発性歯肉増殖症は,カルシウム拮抗剤,抗てんかん剤あるいは免疫抑制剤の服用者の一部に認められる副作用である.歯肉増殖症の発症機構についてこれまでにさまざまな報告がなされてきたが,近年,歯肉増殖症は歯肉線維芽細胞におけるα2インテグリン発現の抑制とコラーゲンファゴサイトーシスの抑制を介したコラーゲン線維の蓄積により発症することが示された.しかしながら,歯肉増殖症の発症率が報告により異なっている原因については不明である.いくつかの疾患において,その発症に関与する遺伝的素因として一塩基多型が知られている.筆者らは,α2インテグリン+807C/T遺伝子多型が歯肉増殖症の発症と関連することを示したが,この遺伝子多型はアミノ酸変異を生じないため発症との直接の関与は考えられず,+807の近傍のほかの一塩基多型との協調関与の可能性が考えられた.そこで本研究では,歯肉増殖症の発症と+807遺伝子多型と連鎖不平衡の関係にあるα2インテグリン+1648G/A遺伝子多型との関連について検討を行った.被験者は,徳島大学病院および新潟大学病院に通院するカルシウム拮抗剤,フェニトイン,あるいはサイクロスポリン服用患者98名を対象とした.歯肉増殖はMcGawらの基準に基づき判定した.末梢血サンプルは増殖症患者45名(増殖症群)と非増殖症患者53名(非増殖症群)から採取した.血液からゲノムDNAを抽出し,α2インテグリン+1648遺伝子部位についてシークエンス分析を行った.その結果,α2インテグリン+1648G/Aの遺伝子型分布は,GGが増殖症群と非増殖症群でそれぞれ95.6%と88.7%と著しく高く,GAはそれぞれ4.4%と9.4%,そしてAAは非増殖症群の1例のみであった.また,アレル頻度については+1646Gアレルが増殖症群と非増殖症群でそれぞれ97.8%と94.7%で,+1648Aアレルがそれぞれ2.2%と5.3%であり,両群間には有意な差は認められなかった.これらの結果は,薬物誘発性歯肉増殖症の発症はヒトα2インテグリン+1648G/A遺伝子多型と相関しないことを示している.しかしながら,歯肉増殖症はα2インテグリン+807C/T遺伝子多型と+1648G/A以外のその他の遺伝子多型との協調を介して発症することも考えられる. The Japanese Society of Conservative Dentistry 特定非営利活動法人 日本歯科保存学会 Jpn J Conserv Dent 日歯保存誌 0387-2343 51 4 464 471 20080000 10.11471/shikahozon.51.464 read0017481/misc/39382306 INAGAKI Yuji 稲垣 裕司 OHBA Hirofumi 大場 博史 NAGATA Toshihiko 永田 俊彦 Study on Vascular Complication and Osteopontin Expression in Dental Pulp Tissues of Rats with Type 2 Diabetes Mellitus 2型糖尿病モデルラットにおける歯髄内動脈硬化巣の同定とオステオポンチンの関与 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY 0387-2343 50 191 191 20071005 read0017481/misc/39324700 NINOMIYA Masami 二宮 雅美 TANAKA Hiroaki 田中 宏明 NAGATA Toshihiko 永田 俊彦 A Case Report of Several Anterior Teeth Devitalized by Ameloblastoma in Mandible 下顎正中部のエナメル上皮腫により多数の下顎前歯が失活していた一症例 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY 0387-2343 50 151 151 20070507 read0017481/misc/38324405 INAGAKI Yuji 稲垣 裕司 OHBA Hirofumi 大場 博史 SETO Hiroyuki 瀬戸 浩行 HORIBE Masumi 堀部 ますみ YOSHIDA Kaya 吉田 賀弥 NAGATA Toshihiko 永田 俊彦 Mineralized Tissue Formation and Osteopontin Expression in Dental Pulp Tissues of Rats with Type 2 Diabetes Mellitus 2型糖尿病モデルラットにおける歯髄内石灰化物形成とオステオポンチンの関与 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY 0387-2343 49 131 131 20060421 read0017481/misc/39039289 OHBA Hirofumi 大場 博史 SETO Hiroyuki 瀬戸 浩行 TOKUNAGA Kaku 徳永 格 HAMA Hideki 濱 秀樹 INAGAKI Yuji 稲垣 裕司 HORIBE Masumi 堀部 ますみ NAGATA Toshihiko 永田 俊彦 The Stimulatory Effect of Simvastatin on Bone Mineralization in Rat Calvarial Defects ラット頭蓋骨骨欠損部におけるシンバスタチンの石灰化促進作用 日本歯周病学会会誌 0385-0110 48 185 185 20060331 read0017481/misc/39039271 BANDO Mika 板東 美香 HIROSHIMA Yuka 廣島 佑香 KIDO Jun-ichi 木戸 淳一 KATAOKA Masatoshi 片岡 正俊 NAGATA Toshihiko 永田 俊彦 Microarray analysis of antimicrobial peptide gene expression in human epithelial cells stimulated by IL-1α マイクロアレイを用いたIL-1α刺激ヒト上皮細胞における抗菌ペプチド遺伝子発現の分析 日本歯周病学会会誌 0385-0110 48 142 142 20060331 read0017481/misc/39038996 NINOMIYA Masami 二宮 雅美 NAGATA Toshihiko 永田 俊彦 A Case Report of the Periodontitis Patient with Secondary Osteoporosis and Cleft Lip and Palate 続発性骨粗鬆症および口唇口蓋裂を有する歯周炎患者に対し歯周・矯正治療を行った一症例 日本歯周病学会会誌 0385-0110 48 227 227 20060331 read0017481/misc/26437611 Hiroyuki Seto 瀬戸 浩行 Masami Ninomiya 二宮 雅美 Toshihiko Nagata 永田 俊彦 Alveolar bone resorption in animal models of periodontitis 実験的歯周炎動物モデルにおける歯槽骨吸収の動態 CLINICAL CALCIUM クリニカルカルシウム 16 2 24(248) 30(254) 20060128 read0017481/misc/39312641 HORIBE Masumi 堀部 ますみ SAWA Takamasa 澤 孝賢 KATAOKA Masatoshi 片岡 正俊 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Effect of Growth Factors on Adhesive Function of Tenascin in Cultured Dental Pulp Cells 培養歯髄細胞におけるテネイシンの細胞接着阻害作用および各種因子の影響 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY 0387-2343 47 117 117 20041018 read0017481/misc/39320377 NINOMIYA Masami 二宮 雅美 SETO Hiroyuki 瀬戸 浩行 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Phase II Clinical Trial of Periodontal Regeneration by KCB-1D (FGF-2) : A Successful Case Report of Periodontal Regeneration by KCB-1D (FGF-2) KCB-1D (FGF-2) による第II相歯周組織再生試験 : KCB-1Dにより著明な改善が認められた一症例 日本歯周病学会会誌 0385-0110 46 167 167 20040925 read0017481/misc/39320358 AZUMA Tomoko 東 智子 NINOMIYA Masami 二宮 雅美 AZUMA Hideki 東 秀樹 NAGATA Toshihiko 永田 俊彦 A case report of a tooth replantation using Emdogain^( !R)Gel エムドゲイン^【○!R】ゲルを用いた歯牙再植術の一症例 日本歯周病学会会誌 0385-0110 46 164 164 20040925 read0017481/misc/34817038 HORIBE Masumi SAWA Takamasa KATAOKA Masatoshi KIDO Jun-ichi NAGATA Toshihiko Regulation of tenascin expression in cultured rat dental pulp cells Odontology : Official journal of the Society of the Nippon Dental University = 歯学 1618-1247 92 1 22 26 20040901 read0017481/misc/38561141 WADA Chie 和田 智恵 SETO Hiroyuki 瀬戸 浩行 KATAOKA Masatoshi 片岡 正俊 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Cyclosporin A Changes Bone Matrix Protein Expression in Rats サイクロスポリンA投与ラットにおける骨基質蛋白の遺伝子発現の変化 日本歯周病学会会誌 0385-0110 46 129 129 20040425 read0017481/misc/38561118 HAMA Hideki 濱 秀樹 AZUMA Hideki 東 秀樹 SETO Hiroyuki 瀬戸 浩行 NINOMIYA Masami 二宮 雅美 OHISHI Keiji 大石 慶二 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Association of Activated TGF-β1 on Inhibition of Osteoblastic Differentiation by Enamel Matrix-Derived Protein エナメルマトリックス蛋白による骨芽細胞分化抑制作用および活性型TGF-βの関与 日本歯周病学会会誌 0385-0110 46 114 114 20040425 read0017481/misc/12942538 Substance P enhances the inhibition of osteoblastic cell differentiation induced by lipoplysaccharide from Porphyromonas gingivalis. Journal of Periodontology 75(7), 974-981 null null 20040000 10.1902/jop.2004.75.7.974 read0017481/misc/12942537 A. Suryono Jun-Ichi Kido Noriko Hayashi Masatoshi Kataoka Toshihiko Nagata Effect of Porphyromonas gingivalis lipopolysaccharide, tumor necrosis factor-α, and interleukin-1β on calprotectin release in human monocytes Journal of Periodontology 0022-3492 74 12 1719 1724 20031200 10.1902/jop.2003.74.12.1719 14974811 2-s2.0-0842280604 read0017481/misc/39324504 WADA Chie 和田 智恵 SETO Hiroyuki 瀬戸 浩行 KATAOKA Masatoshi 片岡 正俊 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Cyclosporin A Changes Osteonectin Expression on Bone Remodeling of Rats サイクロスポリンA投与ラットの骨リモデリングにおけるオステオネクチンの発現変化 日本歯周病学会会誌 0385-0110 45 119 119 20030925 read0017481/misc/39324502 Suryono スルヨノ KIDO Jun-ichi 木戸 淳一 HAYASHI Noriko 林 能理子 KATAOKA Masatoshi 片岡 正俊 NAGATA Toshihiko 永田 俊彦 Effects of Noradrenaline and Cortisol on Calprotectin Expression in Human Monocytic Cells ノルアドレナリンとコルチゾールがヒト単球由来細胞のカルプロテクチン発現に及ぼす影響 日本歯周病学会会誌 0385-0110 45 109 109 20030925 read0017481/misc/39324483 HAYASHI Noriko 林 能理子 KIDO Jun-ichi 木戸 淳一 Suryono スルヨノ KATAOKA Masatoshi 片岡 正俊 NAGATA Toshihiko 永田 俊彦 Regulation of Calprotectin Expression by IL-1α and TGF-β in Human gingival Epithelial Cells IL-1αおよびTGF-βがヒト歯肉上皮細胞のカルプロテクチン発現に及ぼす影響 日本歯周病学会会誌 0385-0110 45 143 143 20030925 read0017481/misc/35193388 NINOMIYA Masami 二宮 雅美 NAGATA Toshihiko 永田 俊彦 A Case Report of the Patient with Malalignment Following Severe Nifedipine-induced Gingival Overgrowth 重度のニフェジン歯肉増殖症により歯列不正が生じたと思われ症例 日本歯周病学会会誌 0385-0110 45 170 170 20030405 read0017481/misc/34899592 AZUMA Tomoko 東 智子 CHO Gota 張 剛太 SUZUKI Keizo 鈴木 敬三 YOSHIDA Kaya 吉田 賀弥 HORIBE Masumi 堀部 ますみ NAKAMURA Teruo 中村 輝夫 NINOMIYA Masami 二宮 雅美 OHISHI Keiji 大石 慶二 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Epidemiologic Study of Periodontal Conditions in Patients Undergoing Diabetic Nephropathy 糖尿病性腎症患者の歯周病罹患に関する疫学的研究 日本歯周病学会会誌 0385-0110 45 109 109 20030405 read0017481/misc/12942536 Calprotectin release from human neutrophils is induced by Porphyromonas gingivalis lipopolysaccharide via the CD-14-Toll-like receptor-nuclear factor kB pathway Journal of Periodontal Research 38(6), 557-563 null null 20030000 10.1034/j.1600-0765.2003.00691.x read0017481/misc/12942535 Decreased expression of α2 integrin in fibroblasts isolated from cyclosporin A-induced gingival in rats Journal of Periodontal Research 38(5), 533-537 null null 20030000 10.1034/j.1600-0765.2003.00692.x read0017481/misc/12942534 Okadaic acid stimulates the expression of receptor activator of nuclear factor-kappa B ligand (RANKL) in mouse osteobrastic cells Biochemical Research 14(2), 126-132 null null 20030000 read0017481/misc/38091907 Kataoka Masatoshi 片岡 正俊 Shimizu Yasuki 清水 保樹 Seto Hiroyuki 瀬戸 浩行 Wada Chie 和田 智恵 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 Nifedipine inhibits apoptosis in gingival epithelial cells in rats B-21-15 : 30 ニフェジピン投与ラットにおける歯肉上皮細胞のアポトーシス抑制 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 44 90 90 20020930 read0017481/misc/38092861 Kirino Akinori 桐野 晃教 Asano Masahiro 浅野 将宏 Oishi Mika 大石 美佳 Asahara Yoji 淺原 洋士 Akimaru Noriko 明丸 倫子 Hama Hideki 濱 秀樹 Syryono スルヨノ Tada Jun 多田 淳 Shionoya Akemi 塩野谷 明美 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 A Case Report on Early Onset Periodontitis Maintaining Inactive Phase after Puberty P-1 思春期後非活動期に入ったと思われる早期発症型歯周炎の一症例 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 44 150 150 20020325 read0017481/misc/38092362 Ohishi Keiji 大石 慶二 Ohishi Mika 大石 美佳 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 Phenotypical change of periodontal ligament cells co-cultured with Hertwig's epithelial root sheath cells C-11-9 : 50 ヘルトビッヒ上皮鞘細胞と歯根膜細胞の共存培養における形質の変化 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 44 128 128 20020325 read0017481/misc/12942533 Application of enamel matrix derivative in autotransplantation of an impacted maxillary premolar. A case report. Journal of Periodontology 73(3),346-351 null null 20020000 10.1902/jop.2002.73.3.346 read0017481/misc/12942532 Nifedipine induces gingival epithrial hyperplasia in rats through inhibition of apoptosis. Journal of Periodontology 73(8),861-867 null null 20020000 10.1902/jop.2002.73.8.861 read0017481/misc/12942531 Effects of TGF-β on extracellular matrix formation in rat clonal dental pulp cells. Dentistry in Japan 38(1),44-50 null null 20020000 read0017481/misc/38089636 Ninomiya Masami 二宮 雅美 Yamauchi Noriyuki 山内 規進 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 A Case Report of Multiple Hypercementosis Observed in Whole Mandibular Teeth D-25 下顎全歯に認められた著名なセメント質過形成の一症例 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 43 176 176 20010930 read0017481/misc/38089624 Azuma Hideki 東 秀樹 Ikeda Dai 池戸 大 Suryono スルヨノ Kataoka Masatoshi 片岡 正俊 Kido Jun-ich 木戸 淳一 Nagata Toshihiko 永田 俊彦 Relationship between inhibitory effects of differentiation and stimulatory effects of cytokine production in cultured osteoblastic cells B-28-9 : 20 骨芽細胞培養系におけるサブスタンスPの分化抑制作用とサイトカイン産生促進との関連 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 43 139 139 20010930 read0017481/misc/38089564 Seto Hiroyuki 瀬戸 浩行 Wada Chie 和田 智恵 Kataoka Masatoshi 片岡 正俊 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 The effect of cyclosporin A on bone amount of rat tibiae B-33-10 : 20 サイクロスポリンA投与ラットにおける脛骨の骨量変化について The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 43 144 144 20010930 read0017481/misc/38098046 Ohichi Keiji 大石 慶二 Zeichner-David M. kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 Culture and characterization of Hertwig's epithelial root sheath cells B-12-11 : 10 ヘルトビッヒ上皮鞘細胞の分離培養とその性質 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 43 98 98 20010330 read0017481/misc/12942530 Immunohistochemical localization of osteopontin in human pulp stones. Journal of Endodontics 27(4),269-272 null null 20010000 read0017481/misc/39283511 HORIBE Masumi 堀部 ますみ SAWA Takamasa 澤 孝賢 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Effects of Various Growth Factors on the Synthesis of Tenascin in Cultured Clonal Dental Pulp Cells 培養歯髄細胞のテネイシン発現に及ぼす各種因子の影響 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY 0387-2343 43 115 115 20001017 read0017481/misc/39282820 NINOMIYA Masami 二宮 雅美 AKAZAWA Kayoko 赤沢 佳代子 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Hypercementosis associated with Chronic Apical Periodontitis in Mandibular First Molars 慢性根尖性歯周炎の進行に伴って形成されたセメント質肥大の経時的変化 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY 0387-2343 43 177 177 20001017 read0017481/misc/38705012 Ninomiya Masami 二宮 雅美 Ishimoto Tomoko 石本 智子 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 Application of EMDOGAIN^>[ !R]< in Autotransplantation of an Impacted Upper Premolar : A case Report D-27 埋伏上顎小臼歯の再植術にエムドゲイン^>[○!R]<を応用した一症例 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 42 194 194 20000925 read0017481/misc/39238315 KADONO Hiroyuki 角野 裕行 KIDO Junichi 木戸 淳一 ASAHARA Yoji 淺原 洋士 SAWA Takamasa 澤 孝賢 KATAOKA Masatoshi 片岡 政俊 NAGATA Toshihiko 永田 俊彦 Inhibition of Osteoblastic Cell Differentiation by Lipopolysaccharide from Porphyromonas gingivalis and Its Mechanism Porphyromonas gingivalis由来リポ多糖による骨芽細胞の分化抑制作用およびその機構解明に関する研究 日本歯科保存学雑誌 0387-2343 43 3 592 601 20000630 read0017481/misc/38090193 Ohba Takanobu 大場 堂信 Akazawa Kayoko 赤沢 佳代子 Nakamura Teruo 中村 輝夫 Ninomiya Yohsuke 二宮 洋介 Akimaru Noriko 明丸 倫子 Ishimoto Tomoko 石本 智子 Kirino Akinori 桐野 晃教 Tono Sayuri 戸野 早由利 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 Periodontal Conditions of Patients Who Have Been on Renal Dialysis D-14 人工透析患者の歯周疾患罹患状況について The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 42 151 151 20000415 read0017481/misc/26437582 Ikedo Dai 池戸 大 Asahara Yoji 淺原 洋士 Sawa Takamasa 澤 孝賢 Ohishi Keiji 大石 慶二 Yamauchi Noriyuki 山内 規進 Simizu Yasuki 清水 保樹 Ohishi Mika 大石 美佳 Ninomiya Masami 二宮 雅美 Kataoka Masatoshi 片岡 正俊 Sinohara Hiroyuki 篠原 啓之 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 Stimulatory Effects of Phenytoin on Osteoblastic Differentiation in Cultured MC 3 T 3-E 1 Cells 培養骨芽細胞MC3T3-E1におけるフェニトインの分化促進作用(共著) Phenytoin (diphenylhydantoin, DPH) , an anticonvulsant drug for epileptic patients, has several adverse effects that include calvarial thickening and coarsening of the facial features. These effects often occur with chronic DPH therapy. While previous studies have shown DPH to heve an anabolic action on bone cells in vivo and in vitro, the basis of these effects is not fully understood. In this study, the effect of DPH on osteoblastic differentiation in cultured MC 3 T 3-E I cells was investigated by measuring mineralized bone nodule (BN) formation, alkaline phosphatase (ALPase) activity, cell growth, and the expression of matrix proteins, such as osteocalcin (OCN), and osteopontin (OPN). Continuous treatment of MC 3 T 3-E I cells with DPH for 20 days increased the BNS (9.1 to 19.7-fold) in a dose-dependent manner for concentrations of 10-50μM DPH. ALPase activity was also stimulated by DPH (1.4-3.2 hold) in a dose-dependent manner for concentrations of 10-100 μM. Although a concentration 50pM DPH increased the DNA content of the cells on days 5 , 10 , and 15 , the final cell saturation density was not affected by any concentration of DPH. When DPH was added at two different culture stages, days 1-10 (growth stage) and days 11-20 (matrix development stage) , BN formation was markedly stimulated in the case of the latter addition. A maximal effect was observed at a concentration of 200pM DPH. The expression of OCN and OPN mRNA was also augmented by continuous treatment at a concentration of 200 μM DPH on days 1-20, and a remarkable effect was also observed when DPH was added on days 11-20 . These findings demonstrate that DPH stimulates osteoblastic markers, such as BNs, ALPase activity, and OCN and OPN expression, mainly during the stage of matrix development in MC 3 T 3-E I cells. J. Jpn. Soc. Periodontol., 42 : 104-113, 2000. フェニトイン(ジフェニルヒダントイン,DPH)はてんかん治療に用いられている抗けいれん薬であり,副作用の一つとして骨形成促進現象が確認されている．しかしながら,DPHが骨芽細胞に及ぽす影響に関する細胞レベルでの詳細な現象および作用機序は十分に明らかにされていない点が多い．本研究では,DPHが骨芽細胞の分化機能発現に及ぼす影響をMC 3 T 3-E 1細胞培養系を用いて,石灰化骨様結節(BN)形成,アルカリフォスファターゼ(ALPase)活性,DNA量,およびオステオカルシン(OCN)とオステオポンチン(OPN)のmRNA発現を指標として検討した．培養開始からDPHを連続的に20日問作用させた場合,BN形成,ALPase活性はそれぞれ10∼50μM,10∼100μMの範囲で濃度に依存して増加した．細胞増殖に及ぽす影響として,50μM DPHは,5,10,15日目で細胞DNA量を増加させたが,最終的な細胞飽和密度には影響を及ぼさなかった．培養期間を変化させてDPHを作用させると,培養前期(細胞増殖期)に添加した場合よりも培養中期(基質含成期)に添加した場合にBN形成の促進が著明に現れ,200μM DPHで最大効果が認められた．OCN,OPN mRNA発現は200μM DPHを培養開始から20日間連続的に作用させた場合に著明に増強され,その作用はBN同様に培養中期に添加した場合に効果的であった．以上の結果から,DPHはMC 3 T 3-E 1細胞においてALPase,OCN,OPNといった骨芽細胞分化指標の発現促進を経てBN形成を増加させることが明らかとなり,この作用は主に基質合成が活発な培養中期にDPHが効果を発揮した結果であることが示唆された． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 42 2 104 113 20000000 10.2329/perio.42.104 read0017481/misc/12942529 Mast cells are not involved in the development of cyclosporin A-induced gingival hyperplasia : A study with mast cell-deficient mice. Journal of Periodontology 71(7),1117-1120 null null 20000000 read0017481/misc/12942528 The association of calprotectin level in gingival crevicular fluid with gingival index and the activities of collagenase and aspartate aminotransferase in adult periodontitis patients. Journal of Periodontology 71(3),361-367 null null 20000000 read0017481/misc/12942527 Sulfated glycosaminoglycan synthesis and its regulation by transforming growth factor-β in rat clonal dental pulp cells. Journal of Endodontics 26(3),169-171 null null 20000000 read0017481/misc/12942526 Cyclosporin A decreases the degradation of type I collagen in rat gingival overgrowth. Journal of Cellular Physiology 182(3),351-358 null null 20000000 10.1002/(SICI)1097-4652(200003)182:3<351::AID-JCP5>3.0.CO;2-U read0017481/misc/38338211 KADONO Hiroyuki 角野 裕行 KIDO Jun-ichi 木戸 淳一 KATAOKA Masatoshi 片岡 正俊 NAGATA Toshihiko 永田 俊彦 Effect of Bacterial Extract Derived from Porphyromonas gingivalis 381 on Differentiation of Osteoblastic Cells Porphyromonas gingivalis 381株由来菌体抽出画分が骨芽細胞の分化に及ぼす影響 日本歯科保存学雑誌 0387-2343 42 55 55 19991001 read0017481/misc/38338204 SHIMIZU Yasuki 清水 保樹 KATAOKA Masatoshi 片岡 正俊 ASAHARA Yoji 浅原 洋士 KUNIKIYO Kenji 国清 憲志 AZUMA Hideki 東 秀樹 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Relationship between apoptosis and gingival overgrowth in the experimental rats administered nifedipine ニフェジピン投与ラットにおける歯肉増殖症の発現とアポトーシスとの関連 日本歯科保存学雑誌 0387-2343 42 53 53 19991001 read0017481/misc/38090092 Nakamura Teruo 中村 輝夫 Kido Jun-ichi 木戸 淳一 Ninomiya Yohsuke 二宮 洋介 Sugimoto Tomomi 杉本 知美 Ohba Takanobu 大場 堂信 Sawa Takamasa 澤 孝賢 Shimizu Yasuki 清水 保樹 Asahara Yoji 淺原 洋士 Ninomiya Masami 二宮 雅美 Yamauchi Noriyuki 山内 規進 Kataoka Masatoshi 片岡 正俊 Shinohara Hiroyuki 篠原 啓之 Nagata Toshihiko 永田 俊彦 Identification and Determination of Osteopontin and Osteocalcin in Human Gingival Crevicular Fluid A-8-10 : 20 歯肉溝滲出液中のOsteopontinおよびOsteocalcinの同定と定量 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 41 63 63 19990925 read0017481/misc/38090036 Kataoka Masatoshi 片岡 正俊 Kunikiyo Kenji 国清 憲志 Shimizu Yasuki 清水 保樹 Asahara Yoji 淺原 洋士 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 Inhibition of collagen phagocytosis in the cyclosporin A-induced gingival overgrowth in rat B-26-9 : 10 サイクロスポリンA誘発性ラット歯肉増殖症における線維芽細胞のI型コラーゲンのファゴサイトーシスについて The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 41 111 111 19990925 read0017481/misc/38401280 HORIBE Masumi 堀部 ますみ KIDO Jun-ichi 木戸 淳一 KATAOKA Masatoshi 片岡 正俊 SAWA Takamasa 澤 孝賢 NAGATA Toshihiko 永田 俊彦 Expressions of Tenascin and Fibronectin in Clonal Dental Pulp Cells and Effects of TGF-β on their Expressions 培養歯髄細胞におけるテネイシンとフィブロネクチンの発現ならびにTGF-βの影響について 日本歯科保存学雑誌 0387-2343 42 19 19 19990401 read0017481/misc/38401092 KUNIKIYO Kenji 国清 憲志 KATAOKA Masatoshi 片岡 正俊 SHIMIZU Yasuki 清水 保樹 AZUMA Hideki 東 秀樹 ASAHARA Yoji 淺原 洋士 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Expression of keratin in the rat enlarged gingival-epithelium induced by Cyclosporine A サイクロスポリンAによって誘発されたラット増殖歯肉上皮におけるケラチンの発現について 日本歯科保存学雑誌 0387-2343 42 42 42 19990401 read0017481/misc/38090723 ASAHARA Yoji 淺原 洋士 AZUMA Hideki 東 秀樹 KATAOKA Masatoshi 片岡 正俊 SHIMIZU Yasuki 清水 保樹 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Cyclosporine A inhibits apoptosis in the rat gingiva C-5-9 : 40 ラット歯肉組織におけるサイクロスポリンAのアポトーシス抑制作用 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 41 116 116 19990330 read0017481/misc/12942525 Stimulatory effects of phenytoin on osteoblastic differentiation of fetal rat calvaria cells in culture. Bone 25(6),653-660 null null 19990000 10.1016/S8756-3282(99)00222-7 read0017481/misc/12942524 Examination of the roots of paramolar tubercles with computed tomography. Report of 3 cases. Oral Surgery Oral Medicine Oral Pathology Endodontics 88(4),479-483 null null 19990000 read0017481/misc/12942523 Effect of retinoic acid on osteopontin expression in rat clonal dental pulp cells. Journal of Endodontics 25(10),683-685 null null 19990000 read0017481/misc/12942522 Calprotectin in gingival crevicular fluid correlates with clinical and biochemical markers of periodontal disease. Journal of Clinical Periodontology 26(10),653-657 null null 19990000 read0017481/misc/12942521 Distribution of osteopontin and calprotectin as matrix protein in calcium-containing stone. Urologic Research 27(4),238-242 null null 19990000 10.1007/s002400050116 read0017481/misc/12942520 Inhibition of osteoblastic cell differentiation by lipopolysaccharide extract from Porphyromonas gingivalis. Infection and Immunity 67(6),2841-2846 null null 19990000 read0017481/misc/12942519 Pronounced palatal and mandibular tori observed in a patient with chronic phenytoin therapy : a case report. Journal of Periodontology 70(4),445-448 null null 19990000 10.1902/jop.1999.70.4.445 read0017481/misc/38337741 NAKAMURA Teruo 中村 輝夫 KIDO Jun-ichi 木戸 淳一 KIDO Reiko 木戸 玲子 OHISHI KEIJI 大石 慶二 YAMAUCHI Noriyuki 山内 規進 KATAOKA Masatoshi 片岡 正俊 NAGATA Toshihiko 永田 俊彦 Possible Utilization of Calprotectin as a Marker for Periodontal Disease Activity -Correlation with Clinical Marker and Biochemical Marker in Gingival Crevicular Fluid- 歯周疾患活動性指標としてのCalprotectin応用の可能性 -臨床指標および歯肉溝滲出液中の生化学的指標との相関について- 日本歯科保存学雑誌 0387-2343 41 74 74 19981001 read0017481/misc/38337546 OHISHI Mika 大石 美佳 OHISHI Keiji 大石 慶二 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Presence of pulp cavity in maxillary paramolar tubercles. 上顎臼傍結節内にみられた歯髄腔の存在について 日本歯科保存学雑誌 0387-2343 41 169 169 19981001 read0017481/misc/38091403 KADONO Hiroyuki 角野 裕行 YAMAUTI Noriyuki 山内 規進 KATAOKA Masatoshi 片岡 正俊 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Effects of LPS from Porphyromonas gingivalis 381 on mRNA Expression of Bone Matrix Proteins in Cultured Osteoblastic Cells B-8-10 : 10 Porphyromonas gingivalis381株由来LPSが骨基質蛋白のmRNA発現に及ぼす影響 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 40 103 103 19980915 read0017481/misc/38091380 Ikedo Dai 池戸 大 Ohishi Keiji 大石 慶二 Yamauchi Noriyuki 山内 規進 Kataoka Masatoshi 片岡 正俊 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 Effect of phenytoin on osteobiast differentiation B-18-14 : 10 フェニトインが骨芽細胞の分化に及ぼす影響 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 40 113 113 19980915 read0017481/misc/38337452 NINOMIYA Masami 二宮 雅美 OHISHI Mika 大石 美佳 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Immunohistological Study on Matrix Proteins in Human Dental Pulp Stone 歯髄結石中に存在する基質蛋白の免疫組織学的検索 日本歯科保存学雑誌 0387-2343 41 48 48 19980501 read0017481/misc/38094754 Nakamura Teruo 中村 輝夫 Kido Jun-ichi 木戸 淳一 Kido Reiko 木戸 玲子 Ohishi Keiji 大石 慶二 Yamauchi Noriyuki 山内 規進 Kataoka Masatoshi 片岡 正俊 Nagata Toshihiko 永田 俊彦 Usefulness of Calprotectin as a Marker for Periodontal Disease Activity A-20-16 : 20 歯周疾患の活動性指標としてのCalprotectinの有用性 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 40 87 87 19980415 read0017481/misc/38094683 KUNIKIYO Kenji 国清 憲志 KATAOKA Masatoshi 片岡 正俊 SHIMIZU Yasuki 清水 保樹 ASAHARA Yoji 淺原 洋士 NINOMIYA Masami 二宮 雅美 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Expression of type I collagen mRNA in Nifedipine induced gingival overgrowth in rats A-27-9 : 48 ニフェジピン誘発性ラット歯肉増殖症におけるI型コラーゲンのmRNAの経時的発現 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 40 94 94 19980415 read0017481/misc/12942518 Calprotectin, a leukocyte protein related to inflammation, in gingival crevicular fluid Journal of Periodontal Research 33(7),434-437 null null 19980000 read0017481/misc/12942517 cDNA cloning of bovine thrombospondin 1 and its expression in odontoblasts and predentin. Biochimica et Biophysica Acta 1382,17-22 null null 19980000 10.1016/S0167-4838(97)00188-X read0017481/misc/12942516 Prostaglandin E₂ inhibits alveolar bone resorption in experimental periodontitis in hamsters. Dentistry in Japan 34,108-111 null null 19980000 read0017481/misc/39367862 NISHIKAWA Hiroyuki 西川 寛之 NISHIKAWA seiji 西川 聖二 KIDO Junichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 A Study of Extracellular Matrix Protein Expressions and TGF-β Effects in Clonal Dental Pulp Cells in vitro 株化歯髄細胞が合成する細胞外基質タンパク質ならびにTGF-βの影響に関する影響 日本歯科保存学雑誌 0387-2343 40 37 37 19971013 read0017481/misc/39367643 KADONO Hiroyuki 角野 裕行 YAMAUCHI Noriyuki 山内 規進 KATAOKA Masatoshi 片岡 正俊 KIDO Jun-ichi 木戸 淳一 NAGATA Toshihiko 永田 俊彦 Effect of LPS Derived from Porphytomonas gingivalis 381 on Differentiation of Osteoblastic Cells Porphytomonas gingivalis 381株由来LPSが骨芽細胞の分化に及ぼす影響 日本歯科保存学雑誌 0387-2343 40 73 73 19971013 read0017481/misc/38090505 Ninomiya Masami 二宮 雅美 Shimizu Yasuki 清水 保樹 Kunikiyo Kenji 国清 憲志 Kataoka Masatoshi 片岡 正俊 Nishikawa Seiji 西川 聖二 Kido Jun-ichi 木戸 淳一 Ishida Hiroshi 石田 浩 Nagata Toshihiko 永田 俊彦 Histopathological Study of Nifedipine-induced Gingival Overgrowth in Rats B-5 ニフェジピン誘発性歯肉増殖症モデルラットにおける病理組織学的検索 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 39 100 100 19970905 read0017481/misc/38090493 Ikedo Dai 池戸 大 Ohishi Keiji 大石 慶二 Nakamura Teruo 中村 輝夫 Kido Jun-ichi 木戸 淳一 Nagata Toshihiko 永田 俊彦 Role of Bone Sialoprotein in Osteoblasts : Supression of BSP Expression by Anti-senseDNA B-11 骨芽細胞における骨シアロ蛋白の役割 : アンチセンスDNAを用いた蛋白発現の抑制 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 39 106 106 19970905 read0017481/misc/38401864 NAKAKO Keiko 中野 恵子 OHISHI Mika 大石 美佳 SHIRAISHI Yoshifumi 白石 良文 OHBA Takanobu 大場 堂場 SHINOHARA Yoshiyuki 篠原 啓之 KIDO Jun-ichi 木戸 淳一 NISHIKAWA Sesiji 西川 聖二 NAGATA Toshihiko 永田 俊彦 Effect of ProstaglandinE_2 on Alveolar Bone Resorption Induced by Experimental Periodontitis in Hamsters ハムスター歯周炎モデルにおけるプロスタグランディンE_2の歯槽骨吸収に与える影響 日本歯科保存学雑誌 0387-2343 40 55 55 19970501 read0017481/misc/38705450 KATAOKA Masatoshi 片岡 正俊 SHIMIZU Yasuki 清水 保樹 YAMAUCHI Noriyuki 山内 規進 NINOMIYA Masami 二宮 雅美 KIDO Jun-ichi 木戸 淳一 NISHIKAWA Seiji 西川 聖二 NAGATA Toshihiko 永田 俊彦 Expression of type I collagen mRNA in Cyclosporine A induced gingival overgrowth in rats C-6 サイクロスポリンA誘発性ラット歯肉増殖症におけるI型コラーゲンのmRNAの経時的発現 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 39 116 116 19970325 read0017481/misc/12942515 Inhibition of osteoblastic differentiation by conditioned medium derived from the human prostatic cancer cell line PC-3 in vitro. Journal of Cellular Biochemistry 67,248-256 null null 19970000 10.1002/(SICI)1097-4644(19971101)67:2<248::AID-JCB10>3.0.CO;2-B read0017481/misc/12942514 Identification of calprotectin, a calcium binding leukocyte protein, in human dental calculus matrix. Journal of Periodontal Research 32(4),355-361 null null 19970000 read0017481/misc/12942513 Noriyuki Yamauchi Seiji Nishikawa Jun-Ichi Kido Keiji Ohishi Toshihiko Nagata Masatoshi Kataoka Hiroyuki Shinohara Hiroshi Ishida Relationship between the expression of parathyroid hormone receptors and hormonal effect during rat osteoprogenitor cell differentiation Journal of Bone and Mineral Metabolism 0914-8779 15 1 17 22 19970000 10.1007/BF02439450 2-s2.0-0030961136 read0017481/misc/38141139 NINOMIYA Masami 二宮 雅美 NISHIKAWA Seiji 西川 聖二 ISHIDA Hiroshi 石田 浩 NAGATA Toshihiko 永田 俊彦 Comparative Histological Study of Mineralizations after Intramuscular Imalantation of Heat-dentrued Demineralized Bone Matrix Gelatin,Heat-denatured Demineralized Tooth and Cross-linked Collagen 熱変性脱灰骨基質ゼラチン, 熱変性脱灰歯, および架橋コラーゲンの筋肉内移植後に認められた石灰化の組織学的比較 日本歯科保存学雑誌 0387-2343 39 66 66 19961001 read0017481/misc/38091880 NAKANO Keiko 中野 恵子 YAMAUCHI Noriyuki 山内 規進 OHISHI Keiji 大石 慶二 KATAOKA Masatoshi 片岡 正俊 KIDO Jun-ichi 木戸 淳一 NISHIKAWA Seiji 西川 聖二 NAGATA Toshihiko 永田 俊彦 The Effect of Prostagladin E_2 on the Mineralization in Cultured Fetal Rat Calvaria Cells B-6-9 : 50 ラット骨原性細胞培養系におけるプロスタグランディンE_2の基質石灰化に及ぼす影響 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 38 111 111 19960905 read0017481/misc/38089365 KIDO Jun-ichi 木戸 淳一 YAMAUCHI Noriyuki 山内 規進 OHISHI Keiji 大石 慶二 NISHIKAWA Seiji 西川 聖二 ISHIDA Hiroshi 石田 浩 NAGATA Toshihiko 永田 俊彦 UENO Akemichi 上野 明道 Regulatory Factor on Osteoblastic Differentiation Derived from Human Prostatic Cancer Cells B-26 10 : 10 ヒト前立腺癌細胞の培養上清中に存在する骨原性細胞分化調節因子について The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 38 146 146 19960325 read0017481/misc/26437572 Keiko Nakano 中野 恵子 Keiji Ohishi 大石 慶二 Noriyuki Yamauchi 山内 規進 Mika Ohishi 大石 美佳 Hiroyuki Shinohara 篠原 啓之 Masatoshi Kataoka 片岡 正俊 Jun-ichi Kido 木戸 淳一 Seiji Nishikawa 西川 聖二 Hiroshi Ishida 石田 浩 Toshihiko Nagata 永田 俊彦 Stimulatory Effect of Prostaglandin E₂ on the Formation of Mineralized Bone Nodules in Cultures of Fetal Rat Calvaria Cells ラット胎児頭蓋冠由来細胞培養系におけるプロスタグランディンE_2の石灰化促進作用 Prostaglandin (PG) E_2 is known to play significant roles in the local control of bone metabolism and to stimulate both formation and resorption of bone in vivo and in vitro. We previously reported a stimulatory effect of PGE_2 on the formation of bone nodules (BNs) in cultures of fetal rat calvaria (RC) cells and we clarified that PGE_2 at 3×10^<-8>M markedly promoted the differentiation of osteoprogenitor cells in cultures of RC cells. In this study, we investigated the action of PGE_2 on BN formation by mature osteoblasts using an RC cell culture system. Treatment with PGE_2 at 3×10^<-8>M resulted in an increase in the ratio of BN mineralization which was initiated by organic phosphate. However, PGE_2 did not affect alkaline phosphatase activity during the whole experimental period. SDS-PAGE profiles showed that PGE_2 at 3×10^<-8>M increased the content of Ca^<2+>-binding proteins (58 and 62 kDa) in mineralized BN matrix. Western blot analysis revealed that the increased proteins were osteopontin. These data suggest that PGE_2 increased mineralization by stimulating the cellular function of mature osteoblasts in cultures of RC cells. プロスタグランディン(PG) E_2は,骨代謝の局所因子として重要な役割を担っており,in vivoあるいはin vitroにおいて,骨形成と骨吸収を促進するという二面性の作用を併せ持っている．我々はすでにラット胎児頭蓋冠由来細胞(RC細胞)培養系に,3×10^<-8>MのPGE_2を添加することで,骨原性細胞から骨芽細胞への分化が促進することを明らかにしている．本研究では,骨芽細胞に富んだRC細胞培養系を用いて,PGE_2の石灰化に及ぼす影響を調べた．その結果,有機リン酸を添加して形成されるBone Nodule (BN)の石灰化率は,PGE_2を添加することにより促進された．しかしながら,PGE_2はアルカリフォスファターゼ活性には影響を与えなかった．石灰化基質中のカルシウム結合性蛋白は,石灰化開始48時間以後に,58および62kDaのバンドに増強が見られた．また,ウエスタンブロット分析から,PGE_2は石灰化BN基質中のオステオポンティンの量を増加させた．これらのことより,PGE_2はRC細胞培養系において分化した骨芽細胞の機能を促進することによって,BNの石灰化を上昇させることが示唆された． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 J. Jap. Soci. Periodontol. 日本歯周病学会会誌 0385-0110 38 4 484 492 19960000 10.2329/perio.38.484 read0017481/misc/12942512 Pathogenesis of drug-induced gingival overgrowth. A review of studies in the rat model Journal of Periodontology 67,463-471 null null 19960000 read0017481/misc/12942511 Osteotropic factor-stimulated synthesis of thrombospondin in rat dental pulp cells FEBS letters 393,193-196 null null 19960000 10.1016/0014-5793(96)00882-4 read0017481/misc/12942510 Effect of calcium phosphate cement implanted in alveolar bone defects in dogs : Appearance of bone sialoprotein at the early stages of bone regeneration Journal of Hard Tissue Bilogy 5(1),23-30 null null 19960000 read0017481/misc/38401100 KIDO Jun-ichi 木戸 淳一 YAMAUCHI Noriyuki 山内 規進 NAKANO Keiko 中野 恵子 OHISHI Keiji 大石 慶二 OHISHI Mika 大石 美佳 SHINOHARA Hiroyuki 篠原 啓之 KATAOKA Masatoshi 片岡 正俊 NISHIKAWA Seiji 西川 聖二 ISHIDA Hiroshi 石田 浩 NAGATA Toshihiko 永田 俊彦 Effect of Conditioned Medium Derived from Clonal Rat Dental Pulp Cells on the Formation of Calcified Bone Nodules in Fetal Rat Calvalia Cells 株化ラット歯髄細胞培養上清がラット頭蓋冠由来骨原性細胞の石灰化骨様結接形成に及ぼす効果 日本歯科保存学雑誌 0387-2343 38 5 1143 1148 19951031 read0017481/misc/38095634 OHISHI Keiji 大石 慶二 YAMAUCHI Noriyuki 山内 規進 OHISHI Mika 大石 美佳 KIDO Jun-ichi 木戸 淳一 NISHIKAWA Seiji 西川 聖二 ISHIDA Hiroshi 石田 浩 NAGATA Toshihiko 永田 俊彦 Effect of retinoic acid on rat calvaria cell differentiation depends on the differentiated stage of the cells B-15-11 : 30 レチノイン酸が骨芽細胞の分化に及ぼす影響 : 細胞の分化段階で異なる作用について The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 37 105 105 19950906 read0017481/misc/38095627 KIDO Jun-ichi 木戸 淳一 OHISHI Keiji 大石 慶二 YAMAUCHI Noriyuki 山内 規進 SHINOHARA Hiroyuki 篠原 啓之 NISHIKAWA Seiji 西川 聖二 ISHIDA Hiroshi 石田 浩 NAGATA Toshihiko 永田 俊彦 Study of calcium binding proteins in dental calculus : Identifications of osteopontin and calprotectin B-12-11 : 00 歯石中のカルシウム結合性蛋白に関する研究 : オステオポンティン及びカルプロテクチンの同定 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 37 102 102 19950906 read0017481/misc/12942509 Physiological concentrations of retinoic acid suppress the osteoblastic differentiation of fatal rat calvaria cells in vitro. European Journal of Endocrinology 133,335-341 null null 19950000 read0017481/misc/12942508 Regulation of osteopontin expression in osteoblasts. Annals of New York Academy of Sciences 760,223-241 null null 19950000 10.1111/j.1749-6632.1995.tb44633.x read0017481/misc/12942507 Identification of osteopontin in human dental calculus matrix. Archives of Oral Biology 40(10),967-972 null null 19950000 read0017481/misc/12942506 Factors influencing nifedipine-induced gingival overgrowth in rat. Journal of Periodontology 66(5),345-350 null null 19950000 read0017481/misc/38097658 YAMAUCHI Noriyuki 山内 規進 OHISHI Keiji 大石 慶二 NISHIKAWA Seiji 西川 聖二 NAGATA Toshihiko 永田 俊彦 ISHIDA Hiroshi 石田 浩 WAKANO Yoichi 若野 洋一 The relationship between changes in the number of parathyroid hormone(PTH) receptors and PTH action during osteoblastic differentiation in rat calvaria cells A-4-9 : 30 ラット骨原性細胞の分化過程における副甲状腺ホルモンレセプター数の変化とホルモンの影響との相関 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 36 68 68 19940910 read0017481/misc/12942505 Phospholipase A₂ activity in gingival cervical fluid from patients with periodontal disease : a possible marker of disease activity. Mediators of Inflammation 3,17-21 null null 19940000 read0017481/misc/12942504 Effect of prostaglandin E₂ on mineralization of bone nodules formed by rat calvarial cells. Calcified Tissue International 55(6),451-457 null null 19940000 10.1007/BF00298559 read0017481/misc/12942503 1α25-Dihydroxyvitamin D₃ stimulation of osteopontin expression in rat clonal dental pulp cells. Archives of Oral Biology 39(9),775-782 null null 19940000 read0017481/misc/12942502 Clinical evaluation of a potassium nitrate dentifrice for the treatment of dentinal hypersensitivity. Journal of Clinical Periodontology 21(3),217-221 null null 19940000 read0017481/misc/38091032 KAHO Keiko 下保 恵子 YAMAUTI Noriyuki 山内 規進 OHISHI Keiji 大石 慶二 NISHIKAWA Seiji 西川 聖二 NAGATA Toshihiro 永田 俊彦 ISHIDA Hiroshi 石田 浩 WAKANO Yoichi 若野 洋一 Effect of prostaglandin E_2 on the formation of bone nodules in fetal rat calvarial cells in culture B-29-11 : 30 ラット頭蓋冠由来細胞培養系におけるプロスタグランディンE_2の骨様組織形成促進作用 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 35 128 128 19930915 read0017481/misc/12942497 Structure and expression of the mRNA encoding urinary stone protein(osteopontin). The Journal of Biological Chemistry 268,15180-15184 null null 19930000 read0017481/misc/12942496 Nifedipine-indnced gigival overgrowth in the presence or absence of gingival inflammation in rat Journal of Periodontal Research 28,396-403 null null 19930000 read0017481/misc/12942495 Effects of interleukin 1β, tumor necrosis factor and transforming growth factor β on group II phospholipase A₂ activity in rat gingival fibroblasts. Journal of Periodontal Research 28,517-520 null null 19930000 read0017481/misc/12942501 Actions of parathyroid hormone on cultured human dental pulp colls. Jounal of Endontics 18,482-487 null null 19920000 read0017481/misc/12942500 Phospholipase A₂ in rat gingival tissue. Journal of Periodontal Research 27,528-533 null null 19920000 read0017481/misc/12942499 Temporal studies on the tissue compartmentalization of bene sialoprotein(BSP), osteopontin(OPN)and SPARC protein during bone formation in vitro. Journal of Cellular Physiology 152,467-477 null null 19920000 10.1002/jcp.1041520305 read0017481/misc/12942498 Clonal dental pulp cells(RDP4-1, RPC-C2A)synthesize and secrete osteopontin(SPP1,2ar) Biochemical and Biophyscical Research Communications 189,892-898 null null 19920000 10.1016/0006-291X(92)92287-8 read0017481/misc/12942486 The role of 5'-methylthioadenosine on rat calvaria cell differentiation Biochemical and Biophysical Research Communications 182,2 null null 19920000 10.1016/0006-291X(92)91805-Z read0017481/misc/12942492 Effects of parathyroid hormone, 1,25-dihydroxyvitamin D3, and prostaglandin E2 on alkaline phosphatase activity in cultured dental pulp and gingiva cells of bovine calf Journal of Endodontics 17,4 null null 19910000 read0017481/misc/12942491 Nifedipine-induced gingival hyperplasia : A clinical and in vitro study Journal of Periodontology 62,1 null null 19910000 read0017481/misc/12942490 Expression of bone matrix proteins associated with mineralized tissue formation by adult rat bone marrow cells in vitro : Inductive effects of dexamethasone Journal of Cellular Physiology 147,1 null null 19910000 10.1002/jcp.1041470115 read0017481/misc/12942489 Biosynthesis of bone proteins by fetal porcine calvariae in vitro. Rapid association of sulfated sialoproteins and chondroitin sulfate proteoglycan with bone mineral Matrix 11,2 null null 19910000 read0017481/misc/12942488 Regulation of glycosaminoglycan synthesis by parathyroid hormone and prostaglandin E2 in cultured dental pulp cells Journal of Endodontics 17,12 null null 19910000 read0017481/misc/12942487 Biosynthesis of bone proteins SPP-1(secreted phospho-protein-1, osteopontin), BSP(bone sialoprotein)and SPARC in association with mineralized-tissue formation Biochemical Journal 274,2 null null 19910000 read0017481/misc/12942494 Effects of insulin and parathyroid hormone on DNA synthesis and ornithine decarboxylase activity in cultured bovine dental pulp Journal of Endodontics 15,3 null null 19890000 read0017481/misc/12942493 Sulphation of secreted phosphoproteins I(SPPI, osteopontin)is associated with mineralized tissue formation Biochemical and Biophysical Research Communications 165,1 null null 19890000 10.1016/0006-291X(89)91059-0 read0017481/misc/26437411 NISHIKAWA Seiji 西川 聖二 UENO Akemichi 上野 明道 ISHIDA Hiroshi 石田 浩 NAGATA Toshihiko 永田 俊彦 HAMASAKI Akihiro 浜崎 章弘 KODA Naohiko 幸田 直彦 KIDO Jun-ichi 木戸 淳一 WAKANO Yoichi 若野 洋一 Studies on Gingival Hyperplasia Induced by Nifedipine : Effects of Nifedipine and EGF on Cell Proliferation in Human Gingival Fibroblast ニフェジピンによる歯肉増殖症に関する研究-ヒト歯肉線維芽細胞におけるニフェジピンおよびEGFの増殖に及ぼす影響． Effects of nifedipine, an anti-anginal drug, and EGF, one of the growth factor which exists in saliva, on growth and DNA synthesis of human gingival fibroblasts were studied. Using standard cell culture technique, gingival fibroblasts were derived from patients taking nifedipine with (Responder) or without (Non-Responder) Dilantin-like gingival hyperplasia. Cells from Responder showed less proliferative activity than those from Non-Responder. The presence of nifedipine during the cultured period did not show any proliferative effects on the cells tested in this experiment. Both the Responder and Non-Responder cells were not significantly sensitive to EGF on DNA synthesis as compared to the control cells. In addition, the content of EGF in saliva did not have any difference between Responder and control. 抗狭心症薬ニフェジピン服用患者で, 歯内増殖症を認めた患者およぴ認めない患者, またニフェジピンを服用している歯周病患者, 各1名から歯肉組織を採取し, 線維芽細胞培養系を確立した後, in vitro でのニフェジピンおよぴEGFの細胞増殖に及ぼす影響を検討した．あわせて患者唾液中のEGFも定量した．ニフェジピン服用患者由来の歯肉線維芽紬胞の培地中にニフェジピンを添加しても細胞増殖率およびDNA合成能の上昇は認められなかった．他の2例についても同様であった．EGF単独添加により, 全ての細胞にDNA合成能の上昇が認められたが, その上昇率には各群の差がなく, さらにEGF存在下でニフェジピンを添加してもEGF単独添加より上昇しなかった．また, 患者唾液中のEGF濃度に差は認められなかった．以上より, 本実験ではニフェジピンの in vitro での増殖促進作用は認められず, EGFとの相互作用も認められなかった． The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会雑誌 0385-0110 28 1 168 175 19860000 10.2329/perio.28.168 3487604 read0017481/books_etc/1798745 Sialoproteins in bone remodiling In"The Biological Mechanisms of Tooth Movement and Craniofacial Adaptation" EBSCO Media 19920000 永田 俊彦 日本歯周病学会学会賞 20170500 永田 俊彦 日本歯科医学会会長賞 20170200 三木康楽賞受賞 19960000