Pentobarbital inhibits glucose uptake, but not water transport by glucose transporter type 3

To understand the mechanisms underlying the neuroprotective efficacy of barbiturates, the effect of pentobarbital on glucose uptake and water transport was determined in Xenopus oocytes expressing glucose transporter type 3 (GLUT3). Pentobarbital induced a 50% concentration-dependent inhibition in glucose uptake, but exerted no effect on water transport by GLUT3. Eadie-Hofstee analysis showed that pentobarbital decreased Vmax significantly, but not Km of GLUT3 for 2-deoxy-D-glucose. Although the protein kinase C (PKC) activator significantly decreased glucose uptake by GLUT3, no additive or synergistic interactions were observed between the PKC activator and pentobarbital. Our results suggest that pentobarbital may play an important role in neuroprotection by inhibition of glucose uptake by GLUT3 by a mechanism involving PKC.


Beta-adrenergic blocker for hyperventilation syndrome in dentistry: A report of three cases


Effects of ketamine on glucose uptake by glucose transporter type 3 expressed in Xenopus oocytes: The role of protein kinase C

We investigated the effects of ketamine on the type 3 facilitative glucose transporter (GLUT3), which plays a major role in glucose transport across the plasma membrane of neurons. Human-cloned GLUT3 was expressed in Xenopus oocytes by injection of GLUT3 mRNA. GLUT3-mediated glucose uptake was examined by measuring oocyte radioactivity following incubation with 2-deoxy-d-[1,2-(3)H]glucose. While ketamine and S(+)-ketamine significantly increased GLUT3-mediated glucose uptake, this effect was biphasic such that higher concentrations of ketamine inhibited glucose uptake. Ketamine (10microM) significantly increased V(max) but not K(m) of GLUT3 for 2-deoxy-d-glucose. Although staurosporine (a protein kinase C inhibitor) increased glucose uptake, no additive or synergistic interactions were observed between staurosporine and racemic ketamine or S(+)-ketamine. Treatment with ketamine or S(+)-ketamine partially prevented GLUT3 inhibition by the protein kinase C activator phorbol-12-myrisate-13-acetate. Our results indicate that ketamine increases GLUT3 activity at clinically relevant doses through a mechanism involving PKC inhibition.

Biochemical and Biophysical Research Communications


Effects of prostaglandin E1 on vascular ATP-sensitive potassium channels.

BACKGROUND: Prostaglandin E1 (PGE1) has been reported to activate ATP-sensitive potassium (KATP) channels, which induces vasorelaxation. However, direct evidence of PGE1 interactions with vascular KATP channels is limited. METHODS: The present study investigated the effects and mechanisms of PGE1 on vascular KATP channels in both isometric tension and patch clamp experiments.Isometric tension experiments were performed in rat thoracic aortic rings without an endothelium. Electrophysiologic experiments were performed using patch-clamp techniques to monitor KATP channels in rat vascular smooth muscle cells. RESULTS: PGE1 significantly decreased the isometric tension in a concentration-dependent manner, which was partially inhibited by pretreating with a KATP channel inhibitor, glibenclamide (1 microM), or an inhibitor of protein kinase A (PKA), Rp-cAMPS (100 microM). Application of PGE1 to the bath solution during cell-attached recordings induced a significant increase in KATP channel activity, whereas PGE1 failed to activate KATP channels in the inside-out patches. The PGE1-induced KATP channel currents in cell-attached patches were abolished by pretreating with Rp-cAMPS (100 microM). CONCLUSIONS: The results indicate that the activation of vascular KATP channels played an important role in the PKA-dependent PGE1-induced vasorelaxation. Furthermore, an electrophysiological experiment demonstrated that PGE1 activated vascular KATP channels via PKA activation.


The Effects of General Anesthetics on Aquaporin 5 Expressed in Xenopus Oocytes

アフリカツメガエル卵母細胞に発現したアクアポリン5に対する全身麻酔薬の作用

Journal of Japanese Dental Society of Anesthesiology


ガム噛みがストレス反応に与える影響

ガム噛みがストレス反応に与える影響


熱中症と悪性症候群の既往をもつ知的障害者の全身麻酔経験

熱中症と悪性症候群の既往をもつ知的障害者の全身麻酔経験


全身麻酔中に短時間の停電に遭遇した経験

全身麻酔中に短時間の停電に遭遇した経験


歯学部学生と卒直後歯科医師に対する救急蘇生教育後の知識およびアンケート調査

歯学部学生と卒直後歯科医師に対する救急蘇生教育後の知識およびアンケート調査


[Anesthetic management of a teenage athlete who developed arrhythmia during general anesthesia].

全身麻酔時に不整脈を呈した高校生スポーツ選手の麻酔経験

A 17-year-old man with fracture of the mandible underwent open fixation under general anesthesia. He was an athlete of the rugby suffering the fracture in a match. His preoperative physical examinations were normal except for I degrees atrioventricular block on electrocardiogram (ECG). During anesthesia, atrioventricular dissociation and frequent premature ventricular contractions were induced by the stimulation of nasotracheal intubation and the administration of atropine for the reversal of muscle relaxation. We thought the cause of the arrhythmia is the athlete's heart which may be vagotonic and may induce vagal reflex or fatal arrhythmia. This case demonstrates that it is necessary to pay attention to chronotropic action associated with the intubation of nasopharynx, the handling of laryngoscope and the usage of drugs for the anesthetic management of the athlete.


Effects of Propofol and Thiamylal on Nicorandil Induced ATP-sensitive Potassium Channel Activities in Cultured Rat Aortic Smooth Muscle Cells

ニコランジルによる培養ラット血管平滑筋細胞のATP感受性Kチャネル活性に及ぼすプロポフォールとチアミラールの影響

Nicorandil, a hybrid ATP-sensitive potassium (K(ATP)) channel opener and nitrate compound, is used clinically for the treatment of angina pectoris. In the present study, we investigated the effects of propofol and thiamylal on sarcolemmal K(ATP) channels activities induced by nicorandil in cultured rat aortic smooth muscle cells. We used inside-out patch clamp configurations to investigate the effects of propofol and thiamylal on nicorandil induced K(ATP) channel activities. K(ATP) channel was not spontaneously activated by patch excision in the absence of intracellular ATP. Application of nicorandil (100 microM) induced a marked activation of KATP channel currents, which was completely blocked by 3 microM glibenclamide, the sulfonylurea that blocks K(ATP) channels. Nicorandil induced KATP channel currents were not significantly inhibited by application of 10 and 100 microM propofol to intracellular surface. However, application of 100 and 300 microM thiamylal to intracellular surface significantly inhibited the nicorandil induced K(ATP) channel currents, with relative channel activities decreasing to 0.65 +/- 0.08 and 0.46 +/- 0.10 of control, respectively. Propofol had no effect on nicorandil induced sarcolemmal KATP channel activities in rat aortic smooth muscle cells, whereas thiamylal significantly inhibited these channel activities at clinically relevant concentrations.


Anesthetic Management of the Patient with Ataxia Telangiectasia

毛細血管拡張性運動失調症患者の全身麻酔経験


低カリウム血症により心電図異常を認めた糖尿病患者の1例

低カリウム血症により心電図異常を認めた糖尿病患者の1例


Characteristics of the trigeminal depressor response in cats

Journal of Japanese Dental Society of Anesthesiology


Acceleration of Calcium-induced Calcium Release Rate in a Japanese Family

家族性にCICR速度亢進を認めた症例


Anaesthetic Study for the Management of Body Fluid and the Efficiency of Preoperative Blood Preparation in Orthognathic Surgery During 10 Years

過去10年間の外科的矯正手術における体液管理と輸血準備の効率化についての検討


全内臓逆位を伴った右胸心患者の全身麻酔経験

全内臓逆位を伴った右胸心患者の全身麻酔経験


We studied the effects of electrical stimulation of the inferior alveolar nerve (IAN) on cardiovascular responses in cats. There was statistical correlation between cardiovascular response and prestimulus mean arterial blood pressure (MABP) and heart rate (HR). A trigeminal depressor response (TDR) was induced when the prestimulus MABP and HR were above 95 mm Hg and 140 beats/min, respectively. We investigated further to identify the vasomotor regulating center and neural transmitters involved in TDR. In the medulla, electrical stimulation of the dorsomedial medulla, the infratrigeminal nucleus (IFT), and the rostral ventrolateral medulla (RVLM) induced a vasopressor response. We confirmed that neurons in the RVLM were retrogradely labeled by wheat germ agglutinin-conjugated horseradish peroxidase injection into the nucleus intermediolateralis of the spinal cord. The vasopressor response induced by IFT stimulation was similar to that induced by IAN stimulation. Vasodepressor responses were induced when the caudal ventrolateral medulla, the nucleus tractus solitarius, the lateral tegmental field, the trigeminal nucleus interpolaris, the trigeminal spinal tract, and the paramedian reticular nucleus were stimulated. These responses, however, were not similar to the vasodepressor response induced by IAN stimulation but were similar to the cardiovascular response induced by vagal afferent stimulation. After spinalization or lesion of the RVLM, MABP and HR decreased and TDR completely disappeared. Inhibitory synaptic ligands and receptors were localized using immunohistochemical techniques. Neurons immunopositive for adrenaline, noradrenaline, and gamma-aminobutyric acid (GABA), and adrenaline alpha(2A), GABA(A), GABA(B), and glycine receptors were distributed along the sympatho-reflexive route including the RVLM and IFT. These results suggest that TDR could be induced as negative feedback to sympathetic hyperactivity whenever MABP and HR are high, because of the inhibitory control of the RVLM.


Extracellular matrix formed by MC3T3-E1 osteoblast-like cells cultured on titanium 2. Collagen fiber formation

Many aspects of the structures formed between titanium and cells and the mechanism of the formation are still unknown. In order to clarify these issues, we analyzed the initial structures formed between titanium and MC3T3-E1 osteoblast-like cells cultured on titanium. The present results indicate that extracellular matrix containing collagen fibers formed at the surface of a calcined layer on the titanium. At first, a hemispherical body and a small secondary spherical structure formed at the surface, followed by more beads to form a string-of-beads structure. These string-of-beads collagen structures elongated and formed spirals. For the first time ever, we directly observed the structure of collagen fiber formed between cultured osteoblasts and titanium with microscopy. Here, we describe the formation mechanism of type I collagen fibers in extracellular bone matrix. Elucidation of the mechanism of collagen fiber formation will aid in the development of controlled tissue regeneration.


Extracellular matrix formed by MC3T3-E1 osteoblast-like cells cultured on titanium 1. Anchor structure

In order to clarify the structure of the extracellular matrix formed by osteoblasts in contact with titanium, we analyzed the structure formed by cultured clonal MC3T3-E1 osteoblasts on a calcified surface of commercial pure titanium, after exfoliating the cells from the surface of the titanium. Collagen fibers formed between neighboring cells and between cells and titanium, in the latter case with one tip of each collagen fiber bound to the cell and the other tip bound to the surface of the titanium embedded in the calcified layer. We called this structure an "anchor structure", because the collagen fibers embedded in the calcified layer anchor the cells to the titanium. This structure may be a reason for the useful biocompatibility between titanium and bone.


The Effect of Lidocaine on Type 3 Facilitative Glucose Transporter Expressed in Xenopus Oocytes

リドカインのグルコーストランスポータータイプ3に対する作用


General Anaesthetic Management of Super Aged Who was Operated Twice during Two Months : A Case Report

2ヶ月で2度，全身麻酔を受けた超高齢者の1例


Postoperative Pain Management with Continuous Intravenous Infusion of Fentanyl and Propofol in a Pediatric Patient with Mental Retardation:a Case Report

フェンタニルとプロポフォールの持続静脈内投与で術後管理を行った精神発達遅滞を伴う小児患者の1例


Clinico-statistical Observations of Outpatients for Dental Treatment at the Anesthetic Clinic in the Hospital of Tokushima University during a 17-year Period

徳島大学歯学部付属病院歯科麻酔科外来における過去17年間の臨床統計的観察

Journal of Japanese Dental Society of Anesthesiology


徳島大学歯学部附属病院歯科麻酔外来における過去17年間の臨床統計的観察

徳島大学歯学部附属病院歯科麻酔外来における過去17年間の臨床統計的観察


肝肺症候群患者の抜歯時の全身管理経験

肝肺症候群患者の抜歯時の全身管理経験


Anesthetic Management for a Patient with Aplastic Anemia

再生不良性貧血を合併する上顎骨亜全摘術の麻酔経験


双方向性リアルタイムの遠隔歯科診療支援システムの構築

双方向性リアルタイムの遠隔歯科診療支援システムの構築

徳島大学総合情報処理センター広報

徳島大学総合情報処理センター広報


Assessment on the sedative effects using bispectral index

鎮静状態の新しい判定方法を使用した経験


No genotoxic effect of propofol in Chinese hamster ovary cells: Analysis by sister chromatid exchanges

In spite of its high placental transfer, propofol is frequently used in general anesthesia and sedation during obstetric and gynecological surgery such as in vitro fertilization. This study investigated whether or not propofol has a genotoxic potential by the sister chromatid exchange assay in vitro. Sister chromatid exchanges induced after exposure to propofol were measured in Chinese hamster ovary cells with and without metabolic activation. After propofol (0.2-20 microg ml(-1)) diluted dimethyl sulfoxide was applied for 2 h with or without S9 mix, the cells having been incubated for two metaphases (34 h) in the presence of 5'-bromo-2-deoxyuridine. N-nitrosodimethylamine and mitomycin C were used as positive controls with and without metabolic activation. The chromosomes were stained with the fluorescence plus Giemsa method, and then sister chromatid exchanges in 50 cells were counted for each concentration. Although increasing concentrations of propofol inhibited cell proliferation, no concentrations of propofol used in this study increased the sister chromatid exchange values, with and without metabolic activation. It was concluded that there was no indication, from the sister chromatid exchange assay in mammalian cells, of a genotoxic effect of propofol and its metabolites.


Propofol is not effective for hyperventilation syndrome


Sturge-Weber 症候群患者の精神鎮静法経験

Sturge-Weber 症候群患者の精神鎮静法経験


リドカインの味覚刺激の種類についての検討

リドカインの味覚刺激の種類についての検討

第28回 日本歯科麻酔麻酔学会総会

第28回 日本歯科麻酔麻酔学会総会


The Effect of Hypoxia on Regional Oral Blood Flow after Local Anesthetics Administration

局所麻酔薬投与下における低酸素血症時の脳および口腔領域の臓器血流量への影響


Usefulness of NAZORCAP(TM) during intravenous sedation with propofol in dentistry

歯科治療時の静脈内鎮静法における呼気ガスサンプリングチューブNAZORCAPTM の有用性


仔牛大動脈内皮細胞による一酸化窒素産生に与える局所麻酔薬の影響

仔牛大動脈内皮細胞による一酸化窒素産生に与える局所麻酔薬の影響


低酸素血症時に局所麻酔薬が臓器血流量へ及ぼす影響について

低酸素血症時に局所麻酔薬が臓器血流量へ及ぼす影響について


Evaluation of a Nitrous Oxide Gas-Barrier Tracheal Tube Cuff,Profile Soft-Seal Cuff

亜酸化窒素抵抗性気管チューブ Profile Soft-Seal Cuffの有用性の検討


INHIBITION OF STYLOGLOSSUS MOTONEURONS DURING THE PALATALLY INDUCED JAW-CLOSING REFLEX


局所麻酔薬が血管内皮細胞へのアルギニン輸送に与える影響

局所麻酔薬が血管内皮細胞へのアルギニン輸送に与える影響


局所麻酔薬が動脈内皮細胞での一酸化窒素に与える影響

局所麻酔薬が動脈内皮細胞での一酸化窒素に与える影響


Propofol Is Effective in Chemotherapy-Induced Nausea and Vomiting:A Case Report with Quantitative Analysis

the International Anesthesia Research Society


Elimination of hypersensitive gagging reaction to dentistry by propofol at subhypnotic doses


Excitation of hypoglossal motoneurons responsible for tongue protrusions is associated with palatally induced jaw-closing reflex


Effects of Midazolam and Flumazenil on Reflex Potentials of the Hypoglossal Nerve Evoked by Stimulation of the Palate


Excitation and inhibition of trigeminal motoneurons by palatal stimulation


Synaptic efficacy of inhibitory synapses and repetitive firing in the reinnervating trigeminal and hypoglossal motoneurons