The effects inhibiting the proliferation of cancer cells by far-infrared radiation (FIR) are controlled by the basal expression level of heat shock protein(HSP) 70A

We developed a tissue culture incubator that can continuously irradiate cells with far-infrared radiation (FIR) of wavelengths between 4 and 20 microm with a peak of 7-12 microm, and found that FIR caused different inhibiting effects to five human cancer cell lines, namely A431 (vulva), HSC3 (tongue), Sa3 (gingiva), A549 (lung), and MCF7 (breast). Then, in order to make clear the control system for the effect of FIR, the gene expression concerned to the inhibition effect by FIR were analyzed. In consequence, basal expression level of HSP70A mRNA was higher in A431 and MCF7 cells than in the FIR-sensitive HSC3, Sa3, and A549 cells. Also, the over expression of HSP70 inhibited FIR-induced growth arrest in HSC3 cells, and an HSP70 siRNA inhibited the proliferation of A431 cells by irradiation with FIR. These results indicate that the effect of a body temperature range of FIR suppressing the proliferation of some cancer cells is controlled by the basal expression level of heat shock protein (HSP) 70A. This finding suggested that FIR should be very effective medical treatment for some cancer cells which have a low level of HSP70. Still more, if the level of HSP70 in any cancer of a patient was measured, the effect of medical treatment by FIR can be foreseen for the cancer.


Specific-wavelength Visible Light Irradiation Inhibits the Growth of Porphyromonas gingivalis.

The effects of laser irradiation on Porphyromonas gingivalis have been reported, but the results are still controversial regarding the efficiency because of the differences of the light sources and irradiation conditions. The aim of this study was to determine the wavelength and irradiation conditions under which the most effective inhibitory effect on P. gingivalis growth was seen without any photosensitizers. Using an Okazaki large spectrograph, monochromatic light spectra ranging from 400 to 700 nm were evaluated to determine which spectra effectively inhibited bacterial growth. Moreover, using a monochromatic 405-nm irradiating device, the effects of various irradiating conditions on P. gingivalis growth were examined. Growth of bacteria irradiated at 400 nm and 410 nm was significantly suppressed compared with a nonirradiated control, whereas wavelengths of 430 nm and longer produced no significant inhibition. A constant energy density of 15 J/cm2 was found to be enough to show an inhibitory effect. Significant inhibition of bacterial growth was found after only 1 min at 50 mW/cm2 irradiation. These results indicate that P. gingivalis growth is specifically suppressed by 405-nm light irradiation, suggesting that visible blue light irradiation is a promising means for eradicating periodontopathogenic bacteria from periodontal lesions.


Effect of FGF-2 and melatonin on implant bone healing: a histomorphometric study .

Melatonin influences the release of growth hormone and cortisol in humans, and it was recently reported that it promoted bone formation. On the other hand, fibroblast growth factor-2 (FGF-2) was reported to facilitate the proliferation of osteoblasts. In the present study, we examined the effect of recombinant human FGF-2 and melatonin on the promotion of osteogenesis around titanium implants. Twenty-four 10-week-old female rats of the Wistar strain received titanium implants in both tibiae. In the experimental groups, 100 mg/kg body weight of melatonin was administered by intraperitoneal injection for 4 weeks after implantation and 10 microg of FGF-2 was locally injected around the implant sites 5 days after implantation. The control groups were administered saline only. In the control group, few newly formed bone could be seen around the implants. It was observed to be in direct contact with the implant surface, but otherwise unmineralized connective tissue was occasionally interposed. In the experimental group, newly formed bone was observed around the titanium implant. In addition, in contrast to the control group, abundant bone trabeculae were seen in the medullary canal region. Bone trabeculae were directly connected to existing cortical bone. These results strongly suggested that melatonin and FGF-2 have the potential to promote osseointegration.

Journal of Materials Science. Materials in Medicine


Possible involvement of stem cell factor and endothelin-1 in the emergence of pigmented squamous cell carcinoma in oral mucosa.

We present here the clinical, morphological and immunohistochemical features of a pigmented squamous cell carcinoma (SCC) in the oral mucosa of the hard palate of a 76-year-old Japanese man. He underwent a partial resection of the maxilla subsequent to radiotherapy. The tumor was typical, moderately well-differentiated SCC but had many melanocytes (melanocytosis) within it. Immunohistochemical analysis for stem cell factor (SCF) and endothelin-1, both of which are known to stimulate proliferation and differentiation of melanocytes, revealed prominent expression of both factors in the neoplastic squamous cells of the pigmented SCC, while the non-pigmented oral SCC showed little sign of either factor. These findings strongly suggest that SCF and endothelin-1 secreted by neoplasmic squamous cells are involved in the emergence of a rare variant of oral SCC.


Histological evaluation of apatite cement containing atelocollagen

Tissue response to apatite cement (AC) containing atelocollagen (AC (ate)) was evaluated using conventional AC (c-AC) as a control material. At one week, the only difference between AC (ate) and c-AC was found in the soft tissue response. With c-AC, a moderate inflammatory response was exhibited: small particles of c-AC were scattered in the cutaneous tissue and many foreign body giant cells were aggregated around the scattered c-AC, whereas AC (ate) showed only a slight inflammatory response with few foreign body giant cells. In terms of bone tissue response, difference between AC (ate) and c-AC was observed at four weeks. New bone formation was observed along the cement at the edge of the pre-existing cortical bone in both c-AC and AC (ate). However, in the case of AC (ate), more abundant and thicker new bone was formed along the cement in the bone marrow when compared with c-AC.


Osteopetrosis complicated by osteomyelitis of the mandible: a case report including characterization of the osteopetrotic bone

A case of a 53-year-old Japanese man with osteopetrosis complicated by osteomyelitis of the mandible is presented. The patient experienced frequent exacerbations and remissions of osteomyelitis of the mandible, despite undergoing several surgical debridements and sequesterectomies with appropriate antimicrobial therapy, for 3 years. Finally, the patient underwent mandibular segmental resection followed by reconstruction with a titanium reconstruction plate. Fifty-one months after surgery there is no evidence of recurrent osteomyelitis of the mandible, suggesting that a more radical surgical approach is preferable for patients with severe complications resulting from osteopetrosis. Also presented here are the histopathological and biochemical features of the osteopetrotic bone. The osteopetrotic cortical bone was morbidly sclerotic with compact and irregular laminations. Degradation of osteocytes in the osteopetrotic bone was due to hypoxia and lack of nutrition resulting from osteosclerosis. There were no significant differences between osteopetrotic and normal bone according to X-ray diffraction, Fourier transform infrared spectroscopy, collagen content or mineral content. Micro-Vickers hardness measurements showed that osteopetrotic bone was significantly harder than normal bone, and the standard deviation of hardness was greater in osteopetrotic bone. Such a loss of integrity in osteopetrotic bone is considered to be a primary reason for the greater risk of a variety of complications such as pathological fracture and refractory osteomyelitis.

International Journal of Oral and Maxillofacial Surgery


矯正牽引装置により治療を行った小児外傷性歯牙陥入の1例

矯正牽引装置により治療を行った小児外傷性歯牙陥入の1例

Japanese Society of Oral and Maxillofacial Traumatology


Intermaxillary Fixation using Alveolar Bone Screws in Mandiblar Fractures --- Report of Five Cases

下顎骨骨折非観血的整復固定症例における顎間固定用スクリューの応用 --- 5症例の検討

日本口腔顎顔面外傷学会誌


A New Animal Raiser: Effect of Low Temperature Narrow Wavelength Far Infrared Radiation on Tumor Growth of A431 Cells

ITE Letters on Batteries, New Technologies & Medicine


Comparison of Resonance Frequency Analyzer and Tooth Mobility Measurement Device for the Evaluation of Dental Implant Stability

インプラントの安定性評価における共鳴振動周波数分析装置と歯牙動揺度測定装置の比較

Journal of Japanese Society of Oral Implantology

日本口腔インプラント学会誌


Development of CO2 Iincubator with Limited Far-Infrared Radiation for Activation of Mitochondrial Metabolism


即時荷重インプラントに関する臨床的検討: 臨床術式と1年経過時の臨床成績

即時荷重インプラントに関する臨床的検討: 臨床術式と1年経過時の臨床成績

日本口腔インプラント学会誌


Clinical Examination of Immediate Loading Implants --- Procedure and 1-year Clinical Results

即時荷重インプラントに関する臨床的検討 --- 臨床術式と1年経過時の臨床成績

日本口腔インプラント学会誌


Proliferation and Differentiation of Cultured MC3T3-E1 Osteoblasts on Surface-layer Modified Hydroxyapatite Ceramic with Acid and Heat Treatments

Effects of functionally gradient calcium phosphate consisting of hydroxyapatite (HAP) and alpha-tricalcium phosphate (alpha-TCP) on proliferation and differentiation of osteoblasts were evaluated using MC3T3-E1 cells. There were no significant differences in the proliferation of MC3T3-E1 cells among HAP-alpha-TCP functionally gradient calcium phosphate, pure HAP, and cell culture plastic wells. mRNA expressions of type I collagen, alkaline phosphate, and osteocalcine were evaluated as indexes of initial; mid-stage, and late-stage osteoblastic differentiation. Basically, HAP-alpha-TCP functionally gradient calcium phosphate and pure HAP enhanced the expressions of the three markers when compared with that of cell culture plastic wells. For type I collagen and alkaline phosphate expressions, HAP-alpha-TCP functionally gradient calcium phosphate showed the same expression level as pure HAP. For osteocalcine expression, HAP-alpha-TCP functionally gradient calcium phosphate showed a higher level than pure HAP. We concluded, therefore, HAP-alpha-TCP functionally gradient calcium phosphate has good potential to be a bone filler material with high osteoconductivity.


A case of Tooth Extraction of the Patient with Bernard-Soulier Syndrome


Immortalization of human dental papilla, dental pulp, periodontal ligament cells and gingival fibroblasts by telomerase reverse transcriptase

Human telomerase reverse transcriptase (hTERT) is catalytic subunit of human telomerase. We studied the immortalization of a series of human dental and periodontal cells by ectopic expression of hTERT and co-expression of hTERT with human papilloma virus 16 (HPV16) or simian virus 40 (SV40). Differentiation abilities of the established cell lines were studied in terms of the mineralized matrix formation and gene expression. We established immortalized gingival fibroblasts by hTERT, dental papilla and periodontal ligament cells by hTERT and HPV16, and pulp cells by hTERT and SV40. The papilla and pulp cells showed mineralization and dentin sialophosphoprotein (DSPP) expression when cultured in the presence of beta-glycerophosphate. The immortalized periodontal ligament cells did not show mineralization or DSPP expression, although expressions of alkaline phosphatase, osteopontin and osteocalcin were detected. These cell lines will be useful tools for studying the repair and regeneration of dental and periodontal tissues and various diseases including odontogenic tumors.


A case of multiple oral cancer with aplastic anemia

再生不良性貧血患者にみられた異時性口腔多発癌の1例

We report a case of multiple oral cancer with aplastic anemia.
The patient was 25-year-old man who was referred to our department for treatment of a mass in the right side of the maxillary gingiva in July 2001. He was given a diagnosis of aplastic anemia at 7 years of age. The maximum amount of mouth opening was 15mm, and a mass with an ulcer was present in the right upper molar region. Computed tomographic examination showed a mass and bone defect in the right maxillary sinus. A biopsy was performed, and the histopathological diagnosis was squamous cell carcinoma. After radiation therapy (external irradiation with 60Co-γ rays), we conducted operation. Red cell and platelet transfusions as well as granulocyte colony-stimulating factor (G-CSF) administration were performed before operation because of decreased numbers of blood cells, and the patient underwent a pertial maxillectomy.
In October 2002, a tumorous lesion appeared in the left side of the mandibular gingiva, and a biopsy revealed squamous cell carcinoma. After the platelet transfusion, resection of the tumor was performed. There has been no evidence of recurrence or metastasis for more than 1 year after surgery.

Japanese Journal of Oral & Maxillofacial Surgery


Effect of Surface Topography on Ssseointegration Establishment of Dental Implant --- Comparison between Smooth and Rough Surfaces

インプラントの表面性状がオッセオインテグレーション獲得に及ぼす影響 --- 滑面と粗面(陽極酸化面)フィクスチャーの比較

日本口腔インプラント学会誌


The novel gene encoding a putative transmembrane protein is mutated in gnathodiaphyseal dysplasia (GDD).

Gnathodiaphyseal dysplasia (GDD) is a rare skeletal syndrome characterized by bone fragility, sclerosis of tubular bones, and cemento-osseous lesions of the jawbone. By linkage analysis of a large Japanese family with GDD, we previously mapped the GDD locus to chromosome 11p14.3-15.1. In the critical region determined by recombination mapping, we identified a novel gene (GDD1) that encodes a 913-amino-acid protein containing eight putative transmembrane-spanning domains. Two missense mutations (C356R and C356G) of GDD1 were identified in the two families with GDD (the original Japanese family and a new African American family), and both missense mutations occur at the cysteine residue at amino acid 356, which is evolutionarily conserved among human, mouse, zebrafish, fruit fly, and mosquito. Cellular localization to the endoplasmic reticulum suggests a role for GDD1 in the regulation of intracellular calcium homeostasis.


Effects of various sterilization methods on the setting and mechanical properties of apatite cement

Sterilization capability is a necessary requirement for any material that is to be used in a medical application. Therefore, it is necessary for apatite cement (AC) to be sterilized. Because there is little information on the sterilization methods of AC, the aims of this investigation were to evaluate the effects of various sterilization methods, including steam, dry heat, ethylene oxide (EtO) gas, and gamma irradiation sterilizations, on the setting and mechanical properties of AC. In the case of steam sterilization, because AC powder aggregated before setting-time measurements, the setting time could not be measured. When the powder was sterilized by dry heat or EtO gas, the setting time was prolonged significantly and the wet diametral tensile strength (DTS) value decreased significantly. Therefore, sterilizations with steam, dry heat, or EtO gas were suggested to be inappropriate methods for AC. Accordingly, the following experiments focused on gamma sterilization. The setting time of AC was retarded with an increase in gamma irradiation dose. The wet DTS value decreased with the increase in gamma irradiation dose. There was no compositional change due to the gamma irradiation. The following tests were carried out in order to examine the effect of the gamma irradiation on the setting reaction of AC in detail. Tetracalcium phosphate [TTCP: Ca(4)(PO(4))(2)O] and dicalcium phosphate anhydrous (DCPA: CaHPO(4)) were separately irradiated, and the cements were produced with the use of irradiated powder and nonirradiated powder. Although the wet DTS value of AC produced from irradiated TTCP and nonirradiated DCPA decreased with increasing gamma irradiation dose, there was no significant difference. In contrast, the wet DTS value of AC produced from irradiated DCPA and nonirradiated TTCP significantly decreased with the increase in gamma irradiation dose. In conclusion, although the detailed mechanism of the delayed setting time and decreased DTS value was not clarified by the present study, it was found that gamma irradiation affected DCPA more than TTCP.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials


Effects of neutral sodium hydrogen phosphate on the setting property and hemostatic ability of hydroxyapatite putty as a local hemostatic agent for bone

Hydroxyapatite (HAP) putty has been reported to have good hemostatic ability and excellent biocompatibility. Although the setting reaction of HAP putty and resulting transformation to HAP is the reason for this excellent biocompatibility, it also limits the handling period. In this study, the relationship between the setting reaction of HAP putty and its hemostatic ability was investigated, where neutral sodium hydrogen phosphate concentration and post-preparation time were used as indexes. A higher concentration of neutral sodium hydrogen phosphate was found to result in decreased hemostatic ability. The hemostatic ability of HAP putty decreased with time after its preparation; thus it was important to use HAP putty within 10 min after its preparation. The adhesive strength of HAP putty to bone increased with time. Therefore reliable hemostasis can be expected with HAP putty. Although the limited handling time is a drawback, HAP putty is thought to have a good potential as a hemostatic agent-it is highly reliable, and has a high hemostatic ability with excellent biocompatibility.


Effects of apatite cements on proliferation and differentiation of human osteoblasts in vitro

Although apatite cement (AC) and sintered hydroxyapatite (s-HAP) are known to show good osteoconductivity, it is not clear whether or not the degree of their osteoconductivity is the same. In addition, it has not been clarified whether or not it is dependent on the type of AC; conventional AC (c-AC), fast-setting AC (fs-AC) or anti-washout AC (aw-AC). The aim of this study was to investigate the effects of ACs on cultured human osteoblasts, as they may provide a useful index of osteoconductivity. Human osteoblasts were cultured on the surfaces of ACs and s-HAP, and were evaluated with respect to cell attachment, proliferation, and differentiation. We found that ACs and s-HAP showed similar cell attachment. No significant difference between ACs and s-HAP was found with respect to the proliferation of osteoblasts. In contrast, we found that the differentiation of osteoblasts was enhanced on the surface of ACs compared with that of s-HAP. However, there was no difference among the types of AC. We therefore concluded that AC may show better osteoconductivity than s-HAP, and that osteoconductivity of AC may be similar, regardless of the type of AC.


エナメル基質タンパクの歯の移植への応用

エナメル基質タンパクの歯の移植への応用


Two Cases of So-Called Kuttner Tumor of Submandibular Gland

顎下腺に発症した いわゆるKuttner腫瘍の2例


A Case of Quadruple Cancer Arising Synchronously in the Qpper Gingiva,Penis,and Kidney 2 Years After Colon Cancer Treatment

大腸癌治療2年後に同時性に上顎歯肉，陰茎および腎に癌を生じた4重複癌の1例


薬物療法を行った顎関節症患者の臨床的検討

薬物療法を行った顎関節症患者の臨床的検討

Journal of the Japanese Society for the Temporomandibular Joint


Effect of the additional installation of implants in the posterior region on the prognosis of treatment in the edentulous mandibular jaw

The aim of this study was to elucidate the effect of the additional installation of implants in the posterior region on the prognosis of treatment in the edentulous mandibular jaw. Fifteen patients who had received implants (Brånemark system, Nobel Biocare, Gotebörg, Sweden) in the edentulous mandible and completed a 1-year follow-up after the fitting of implant-anchored fixed prostheses were selected. In seven patients (Group A), four or five implants were installed between the mental foramina, and in eight patients (Group P), one or two implants, one on each side, were installed in the posterior regions in addition to the implants between the foramina. All implants of both groups achieved osseointegration. In Group A, there was no implant loss after loading. Six implants were lost in five patients of Group P within 1 year after loading. All of them were located in the posterior region. To elucidate whether or not the failure rate of the implants in the posterior region of Group P after loading was especially high, the failures were also compared with 89 implants, which were installed in the posterior region of the mandibles to support implant-anchored fixed partial prosthesis, during the same period (Group C). The cumulative survival rate of the implants of Group P was 60%, while that of the implants of Group C was 100% (P<0.001). When the survival rates of posterior implants with the same length of the two groups were compared, there were significant differences for the 7- and 10-mm-length implants only. These data demonstrate that the posterior implants in Group P are at greater risk. Deformation of the mandible due to jaw movement was thought to be the most likely cause of the implant loss. Therefore, when such modified treatment is chosen, it should be performed with meticulous attention.


顎関節に発生した滑膜性軟骨腫症の1例

顎関節に発生した滑膜性軟骨腫症の1例

Although synovial chondromatosis occurs frequently near the ends of long bones, such as the knee, elbow, and hip joints, it rarely affects the temporomandibular joint.<BR>A 52-year-old woman visited our hospital because of swelling in the left side of the temporomandibular joint and trismus. On magnetic resonance imaging, the lesion was found to be chondroid tissue between the left mandibular fossa and the condylar head. Magnetic resonance imaging revealed a high signal mass measuring 35×40mm around the left condylar head. The clinical diagnosis was synovial chondromatosis. Synovectomy and removal of the mass were performed with the patient under general anesthesia. Histopathologically, the mass was formed by typical chondrocytes and was diagnosed to be synovial chondromatosis. The patient has been followed up for 1 year 4 months. There has been no reccurence of the mass or temporomandibular joint disorder.

Japanese Journal of Oral & Maxillofacial Surgery


Basic fibroblast growth factor and epidermal growth factor reverse impaired ulcer healing of the rabbit oral mucosa


Down-regulation of Wnt-4 and up-regulation of Wnt-5a expression by epithelial-mesenchymal transition in human squamous carcinoma cells

Gene expression of Wnt-1, 2, 3, 4, 5a, 6 and 7a was analyzed by RT-PCR in eleven squamous cell carcinoma (SCC) cell lines and compared with that in two normal oral keratinocyte strains. There appeared to be an inverse relationship between Wnt-4 and Wnt-5a expressions, i.e., Wnt-4 was not expressed in HOC719-NE, HOC313 or TSU cells, while Wnt-5a was strongly expressed only in these cells. These cell lines showed decreased expression of E-cadherin and elevated expression of vimentin accompanied with strong expressions of Snail and deltaEF1, which have been reported to be transrepressors of E-cadherin and to trigger epithelial-mesenchymal transition (EMT), suggesting associations of Wnt-4 with epithelial phenotype and Wnt-5a with mesenchymal phenotype of SCC cells. To study whether the expressions of these Wnt genes are regulated by EMT, we transfected a Snail-expression vector into A431 and OM-1 cells, which express Wnt-4 but not Wnt-5a. The stably Snail-overexpressing clones showed spindle morphology, increased expression of vimentin and decreased expression of E-cadherin accompanied with augmented expression of deltaEF1. In these clones, down-regulation of Wnt-4 and up-regulation of Wnt-5a were clearly observed. These results indicated that Wnt-4 and Wnt-5a are oppositely affected by EMT, and down-regulation of Wnt-4 and up-regulation of Wnt-5a are possible markers of the malignant phenotype of human SCC.


Evaluation of Risk Factors Concerning Loss of Osseointegration of Dental Implant

歯科インプラントのオッセオインテグレーション喪失に関与するリスクファクターの検討

Journal of Japanese Society of Oral Implantology

日本口腔インプラント学会誌


Traumatized Alveolar Bone Defect with Recovery of Oral Functions Using Vertical Alveolar Distraction Osteogenesis and Immediate Loading Implant:A Case Report

垂直的歯槽骨延長と即時荷重インプラントによって機能回復を図った外傷性歯槽骨欠損の1例


下顎骨関節突起骨折の臨床的検討

下顎骨関節突起骨折の臨床的検討


Increased invasion and matrix metalloproteinase-2 expression by Snail-induced mesenchymal transition in squamous cell carcinomas.

Loss of E-cadherin expression is a major characteristic of highly invasive and metastatic cancers. Epithelial-mesenchymal transition (EMT) has been advocated to be a causative mechanism for the suppression of E-cadherin and tumor progression. Snail is a zinc finger transcription factor that triggers the EMT and is one of the recently identified E-cadherin repressors. The reverse correlation of Snail and E-cadherin expressions has been reported in many types of human cancers including squamous cell carcinoma (SCC). In this study, we showed that three E-cadherin negative SCC cell lines had a fibroblastic morphology, strong expressions of vimentin, a mesenchymal marker gene, and Snail. Compared to other E-cadherin positive SCC cells, these cells showed higher invasive ability and expression of MMP-2, a matrix degrading enzyme which has been demonstrated to be highly expressed in invasive cancer cells. Over-expression of Snail in A431 cells resulted in the loss of E-cadherin expression, the change of their morphology to fibroblastic, and the up-regulation of vimentin gene expression, indicating that an EMT was induced by Snail. Furthermore, these cells became more invasive and showed higher levels of MMP-2 activity and its gene expression. Luciferase analysis demonstrated that the MMP-2 promoter activity was induced by Snail transfection and the promoter region from -262 to -411 relative to the transcriptional start site was necessary for this induction. These results indicate that Snail is a new inducer of MMP-2 expression and suggest that the EMT contributes to the increased invasion not only through the inhibition of cell-cell adhesion but also the up-regulation of MMP-2 expression in SCC cells.


Use Experience with an Implant-Type Distractor for Vertical Alveolar Distraction Osteogenesis

垂直的歯槽骨延長におけるインプラント型骨延長器の使用経験

日本口腔インプラント学会誌


Autosomal Dominant Gnathodiaphyseal Dysplasia Maps to Chromosome 11p14.3-15.1

Gnathodiaphyseal dysplasia (GDD) is a syndrome characterized by bone fragility, sclerosis of tubular bones, and cemento-osseous lesions of jawbones. Although some cases of this syndrome exist in families with autosomal dominant inheritance, the underlying gene has never been identified. We analyzed a large four-generation family with GDD by linkage analysis using genomic DNA from nine affected and six nonaffected family members. A genome-wide search using a set of highly polymorphic microsatellite markers showed evidence for linkage to chromosome 11p14.3-15.1. Two-point linkage analysis of microsatellite markers spanning this locus resulted in a maximum logarithm of odds (LOD) score of 2.70 with a recombination fraction (theta) of 0 at D11S1755, D11S1759, and D11S915, and a maximum LOD score of 3.01 at D11S4114 was obtained in multipoint linkage analysis. Haplotype analysis detected no recombination between GDD and six closely linked markers (D11S928, D11S1755, D11S4114, D11S1759, D11S915, and D11S929) and established the candidate interval of 8.7 cM on chromosome 11p for GDD. Although GDD has been considered to be a variation of osteogenesis imperfecta (MIM 166260), our results indicate that this syndrome is a new and distinct disease entity from other systemic bone diseases. Furthermore, these genetic markers are useful for presymptomatic diagnosis of GDD in some families and for identification of the GDD gene.


Clinical statistical study of jaw deformity patients at the Department of Orthodontics, Tokushima University Dental Hospital

徳島大学歯学部附属病院矯正歯科における顎変形症患者の臨床統計学的検討


クローズドロックに対するパンピング·マニピュレーション，上関節腔洗浄療法について


骨折治癒過程におけるレプチンの役割

骨折治癒過程におけるレプチンの役割

日本口腔顎顔面外傷学会雑誌

日本口腔顎顔面外傷学会雑誌


Evaluation of Clinical Factors Conserning Osseointegration Establishment of a Dental Implant

歯科インプラントのオッセオインテグレーション獲得に関与する臨床的要因の検討

日本口腔インプラント学会誌


6歳児の下顎骨に発生した若年性(侵襲性)化骨性線維腫の1例

6歳児の下顎骨に発生した若年性(侵襲性)化骨性線維腫の1例

Pediatric Oral and Maxillofacial Surgery


広範な口腔粘膜病変を初発症状とした腫瘍随伴性天疱瘡の1例

広範な口腔粘膜病変を初発症状とした腫瘍随伴性天疱瘡の1例

Journal of Japanese Society for Oral Mucous Membrane


The in vitro antibiotic release from anti-washout apatite cement using chitosan (aw-AC(chi)) was investigated in a preliminary evaluation. Flomoxef sodium was employed as the antibiotic and was incorporated into the powder phase aw-AC(chi) at up to 10%. The setting times were measured for aw-AC(chi) containing various amounts of flomoxef sodium. X-ray diffraction (XRD) analysis was also conducted for the identification of products. To evaluate the drug release profile, set aw-AC was immersed in saline and the released flomoxef sodium was determined at regular intervals. The setting time was prolonged slightly with the addition of flomoxef sodium. The difference at 10% flomoxef sodium (0% vs. 10%) was not significant (p>0.05), and can be negligible in clinic. The XRD analysis revealed that formation of hydroxyapatite (HAP) from aw-AC(chi) was reduced, even after 24 h, when the aw-AC(chi) contained flomoxef sodium at 8% or more. The flomoxef sodium release from aw-AC(chi) showed the typical profile observed in skeleton type drug delivery system (DDS). Changing the concentration of chitosan can control the rate of drug release from aw-AC. Therefore, we conclude that aw-AC(chi) is a good candidate for potential use as a DDS carrier that may be useful in surgical operations.

Journal of Materials Science. Materials in Medicine


高度な骨性癒着をきたした顎関節強直症の1例

高度な骨性癒着をきたした顎関節強直症の1例

Journal of the Japanese Society for the Temporomandibular Joint


Establishment of Immortalized Human Oral Keratinocytes by Gene Transfer of a Telomerase Component

テロメラーゼ遺伝子導入による不死化ヒト口腔粘膜上皮細胞の樹立


Application of Enamel Matrix Derivative in Autotransplantation of an Impacted Maxillary Premolar

The success of tooth transplantation or replantation depends on the viability of periodontal ligament in the planted tooth. Mechanical injury to periodontal tissues frequently results in dental root resorption and dental ankylosis, which leads to the failure of transplantation or replantation. Enamel matrix derivative (EMD) has been recently used to induce periodontal regeneration. In this report, we show a clinical case of EMD application in the transplantation of an inversely impacted and immature tooth. An impacted second premolar was found in the right maxilla of a 16 year-old girl. The tooth was inversely impacted and the dental root was incomplete. When transplantation was carried out, EMD was applied to the periodontal tissues of the extracted premolar. The tooth was fixed at the correct position and the clinical condition was followed for evaluation for 6 months. Radiographs after 3 months exhibited new bone formation surrounding the transplanted tooth. After 6 months, considerable growth of dental root was evident, periodontal ligament-like radiolucency appeared, the vital reaction of the planted tooth was detected, and there were no signs of root resorption or ankylosis. Short-term results from this case indicate that EMD application was effective in the transplantation of an inversely impacted and immature tooth and that EMD might contribute to the growth of dental root and to the prevention of ankylosis.


Potential role of leptin in endochondral ossification

Leptin, a 16-kD circulating hormone secreted mainly by white adipose tissue, is a product of the obese (ob) gene. Leptin acts on human marrow stromal cells to enhance differentiation into osteoblasts and inhibit differentiation into adipocytes. Leptin also inhibits bone formation through a hypothalamic relay. To obtain a better understanding of the potential role of leptin in bone formation, the localization of leptin in endochondral ossification was examined immunohistochemically. High expression of leptin was identified in hypertrophic chondrocytes in the vicinity of capillary blood vessels invading hypertrophic cartilage and in a number of osteoblasts of the primary spongiosa beneath the growth plate. The hypertrophic chondrocytes far from the blood vessels were negative for leptin. Moreover, we detected the production and secretion of leptin by a mouse osteoblast cell line (MC3T3-E1) and a mouse chondrocyte cell line (MCC-5) by RT-PCR, immunocytochemistry, and Western blotting. Leptin enhanced the proliferation, migration, tube formation, and matrix metalloproteinase-2 (MMP-2) activity of human endothelial cells (HUVECs) in vitro. These findings suggest the possibility that leptin exerts its influence on endochondral ossification by regulating angiogenesis.

The Journal of Histochemistry and Cytochemistry


Fabrication of Zn containing apatite cement and its initial evaluation using human osteoblastic cells

Recently, the effects of Zn2+ on osteogenesis stimulation have become major topics in the research fields of bone formation and organism essential elements. Based on the fundamental finding of Zn2+ with respect to osteogenesis stimulation, Ito et al. have prepared Zn doped beta-tricalcium phosphate (ZnTCP) and have reported that ZnTCP enhances the proliferation of MC3T3-E1 cells. In this investigation, we studied the effects of ZnTCP added to apatite cement (AC) with respect to its setting reaction and proliferation of human osteoblastic cells as an initial evaluation for the feasibility of AC containing ZnTCP. Compositional analysis using powder X-ray diffractometer revealed that ZnTCP shows no reactivity with the setting reaction of AC. As a result, the mechanical strength of set AC decreased increasing amounts of added ZnTCP as if ZnTCP acts as a pore in AC. The setting time of AC was not affected by addition of ZnTCP up to 10%. When AC containing ZnTCP was immersed in alpha-MEM containing 10% bovine serum, Zn2+ was released from AC. Larger amounts of Zn2+ were released from AC containing larger amounts of ZnTCP. When human osteoblastic cells were incubated on the surface of AC discs, proliferation of human osteoblastic cells was significantly increased on the surface of AC that contained 5% ZnTCP when compared with that containing no ZnTCP. In contrast, proliferation of human osteoblastic cells decreased on the surface of AC that contained 10% ZnTCP when compared with that free from ZnTCP; indicating cytotoxicity. We concluded therefore, that addition of ZnTCP to AC is useful to enhance the osteoconductivity of AC when release of Zn2+ can be carefully regulated.


Identification of cis-elements regulating expression of Fgf10 during limb development.

Fibroblast growth factor 10 (FGF10) is known to be expressed in limb mesenchymal cells and to function as a mesenchymal signaling factor involved in epithelial-mesenchymal interactions during limb development. To elucidate regulation of Fgf10 expression, we isolated the promoter region of Fgf10 containing its 2.0 kb upstream 5'-fragment from the initiation codon and its 0.9 kb downstream fragment. Transcriptional activity of the fragment was examined with transgenic mice, using a lacZ-reporter system. Although no significant expression of the reporter gene was observed for the 0.2 kb 5'-fragment, expression was detected in the apical ectodermal ridge of the limb bud and developing cartilage of the limb for the 2.0 kb and 0.7 kb 5'-fragments, respectively. From comparison of the mouse sequences of the 2.0 kb fragment with corresponding sequences of human and chicken Fgf10, we identified 17 conserved putative enhancer motifs for AER expression and other unidentified expressions. For limb cartilage expression, we found putative enhancer sequences conserved among the three species in the 0.7 kb 5'-fragment. In the fragment, three DNA binding motifs were identified in the mouse and human sequence, although they are not conserved in the corresponding chicken sequence.

The International Journal of Developmental Biology


Autotransplantation of Impacted Teeth and Wisdom Teeth


Effects of powder phase sterilization on basic properties of apatite cement


Evaluation of Feasibility of Hydroxyapatite Putty as a Local Hemostatic Agent for Bone.

Although bone wax is known to cause an inflammatory reaction in soft tissue and a delay of bone healing, it is usually the first choice to arrest bleeding from bone. Hydroxyapatite (HAP) putty is proposed here. This study evaluated the feasibility of HAP putty with respect to whether or not it has potential value as a hemostatic agent for bone. First, the adhesive strength of HAP putty to bone was evaluated. The adhesive strength of HAP putty to wet bone was much higher when compared with that of bone wax, even though the adhesive strengths of HAP putty and bone wax to dry bone were equivalent. Next, to evaluate the hemostatic ability of HAP putty, bony defects were made in rabbits. HAP putty could arrest bleeding from bone within 3 min. However, when the hemostasis was performed with bone wax, bleeding was arrested within 8 min. Thus, HAP putty showed better hemostatic ability than bone wax. Finally, the tissue response to HAP putty in rabbit subcutaneous tissue was evaluated. Histological observation revealed a slight inflammatory response around HAP putty, whereas bone wax was surrounded by moderate inflammatory tissue. In conclusion, HAP putty has good potential value to be a hemostatic agent for bone because it has strong adhesion, good hemostatic ability, and biocompatibility.

Journal of Biomedical Materials Research


A case of aspergillosis of the maxillary sinus: discussion of the usefulness of serological examinations

上顎洞に発症したアスペルギルス症の1例 : 血清学的検査の有用性についての考察

Aspergillosis is caused by infection with Aspergilli. It is often difficult to make an exact diagnosis before identification of Aspergilli by microbial examination because aspergillosis is difficult to differentiate from other diseases. We present a case of aspergillosis of the maxillary sinus that could be diagnosed on serological examination for Aspergilli or fungi specific sugar.
A 51-year-old woman visited to our hospital because of swelling in the gingiva of the maxilla. Radical operation of the maxillary sinus was performed for a clinical diagnosis of aspergillosis of the maxillary sinus. Histopathological examination revealed Aspergilli in the extirpated tissue of the maxillary sinus. Serological examinations showed elevated serum levels of galactomannan and β-D-glucan. Galactomannan is a specific marker for Aspergilli, and β-D-glucan is specific for fungi. Both makers fell to normal levels after operation, Suggesting that serological examinations for galactomannan and β-D-glucan are useful in the diagnosis of aspergillosis of the maxillary sinus.

Japanese Journal of Oral & Maxillofacial Surgery


Tissue responses to anti-washout apatite cement using chitosan when implanted in the rat tibia

The tissue response to anti-washout apatite cement using chitosan (aw-AC(chi)) was evaluated by implanting aw-AC(chi) into bone defects of rat tibiae using conventional apatite cement (c-AC) as a control material. During the experimental period up to 16 weeks, the only difference between aw-AC and c-AC was found at two weeks in the tissue response of soft tissue. At two weeks, c-AC showed a moderate inflammatory response; small particles of c-AC were scattered in the cutaneous tissue and many foreign body giant cells were collected around the scattered c-AC, whereas aw-AC showed only a slight inflammatory response and few foreign body giant cells. We found no difference between aw-AC(chi) and c-AC with respect to bone tissue response. Both AC were almost completely surrounded by mature bone at eight weeks. No promotion or reduction of osteoconductivity was observed by chitosan even though it is considered to promote bone formation. We concluded, therefore, that enhancement of bone formation cannot be expected by employing chitosan to obtain anti-washout properties, at least in the concentration used in this study, even though aw-AC(chi) is much more useful than c-AC.

Journal of Materials Science. Materials in Medicine


Bone formation by transplanted human osteoblasts cultured within collagen sponge with dexamethasone in vitro


The stability of mesial and distal bony segments after sagittal split ramus osteotomy using PLLA screws

PLLA製スクリューを用いた下顎枝矢状分割術後の近·遠位骨片の安定性


Effect of added NaHCO3 on the basic properties of apatite cement

Alternation of a composition of set apatite cement (AC) from carbonate-free apatite (CO3-free AP) to carbonate apatite (CO3-AP) may accelerate replacement of the set AC with bone because CO3-AP can be dissolved much faster than CO3-free AP in the weak acidic solution produced by osteoclasts. In this investigation, NaHCO3 was added to the AC component and the effects of added NaHCO3 on cement-setting behavior and on set mass were studied as an initial step for the fabrication of AC, which can be replaced with bone faster than current AC. When NaHCO3 was added to AC, the resultant set mass contained CO3. Although not all CO3 was incorporated in the set AC, the amount of CO3 incorporated into the set AC increased with the amount of added NaHCO3. Powder X-ray diffraction analysis and Fourier transform infrared spectrometer measurements revealed the formation of B-type CO3-AP. The setting time measured in an incubator at 37 degrees C and 100% relative humidity slowed from 30 to 45 min, indicating that NaHCO3 has an inhibitory effect on AP formation. The diametral tensile strength of the set AC decreased significantly with the addition of NaHCO3. Scanning electron microscopic observation revealed that fine crystals were formed in the set AC when NaHCO3 was added to AC. As a result, the crystallinity indices of the set AC measured using X-ray diffraction and Fourier transform infrared spectroscopy decreased with an increase in the amount of added NaHCO3. The dissolution rate of set AC in weak acid, pH 5.5, increased with the amount of added NaHCO3. We concluded that the formation of B-type CO3-AP and the resulting faster dissolution of set AC in weak acidic solution is preferable for the faster replacement of set AC with bone even though the decreased diametral tensile strength value is a shortcoming.


In vitro resorption of three apatite cements with osteoclasts

To evaluate the replacement of apatite cement (AC) with bone, osteoclasts were incubated for 48 h on the surface of three AC types: conventional AC (c-AC), fast-setting AC (fs-AC), and anti-washout AC (aw-AC), using sintered apatite (AP) and cortical bone as control materials. We found osteoclasts attached to the surface of AC and osteoclastic resorption pits after 48 h of incubation for all experimental AC types. In contrast, no resorption pit was observed on the surface of sintered AP although osteoclasts were attached to the surface of sintered AP. There was no significant difference among the types of AC with respect to the resorption area, but the resorption areas were only approximately 1% of that on the surface of cortical bone. We concluded, therefore, that ACs could be replaced with bone regardless of the type but that it takes extensive time for the ACs to be completely replaced with bone.


Expression of telomerase components in oral keratinocytes and squamous cell carcinoma

Telomerase activity was measured using a telomeric repeat amplification protocol (TRAP), and expressions of the telomerase components, telomerase associated protein 1 (hTEP1), human telomerase RNA component (hTR), and human telomerase reverse transcriptase (hTERT) were measured by reverse transcriptase-polymerase chain reaction (RT-PCR) in cultured normal oral keratinocytes and oral squamous cell carcinoma (SCC) cells. Telomerase localization was analyzed by in situ hybridization (ISH) in normal, precancerous and cancerous oral tissues. There was a strong correlation of telomerase activity with the expression levels of hTERT but not with hTEP1 or hTR mRNA in the cultured cells. Not only hTEP1 and hTR but also hTERT expression were detected in the basal cells of normal oral mucosa, and the cells expressing these mRNAs were also seen in the upper layer of leukoplakia of gingiva, and a heterogeneous pattern of expression was observed in the oral SCC tissues. These results indicate that there are at least two steps in the increase of telomerase activity during carcinogenesis in oral squamous cells; a change in distribution of cells expressing these telomerase components and the over-expression of hTERT gene in individual cells.


Reverse correlation of E-cadherin and snail expression in oral squamous cell carcinoma cells in vitro

The loss of E-cadherin expression has been shown to correlate to the invasion and metastasis of many types of carcinomas. We established E-cadherin positive (HOC719-PE) and negative (HOC719-NE) clones from an oral squamous cell carcinoma (SCC). HOC719-PE cells showed epithelial morphology with E-cadherin expression in the cell membrane, whereas HOC719-NE cells demonstrated fibroblastic morphology without E-cadherin expression. In invasion assay and three dimensional culture, HOC719-NE showed much higher invasive ability than HOC719-PE cells. These cells expressed similar levels of mRNAs for alpha- and beta-catenin. However, HOC719-NE cells, but not HOC719-PE cells, showed strong expression of snail, a transcription factor implicated in the differentiation of epithelial cells into mesenchymal phenotype. This reverse expression of snail and E-cadherin was further observed in other SCC cells including HOC313, and TSU cells that we previously reported to show no expression of E-cadherin protein. These results indicated that the expression of snail has a key role for the acquisition of more invasive and metastatic phenotypes of SCC and the clones we reported here will be useful tools for understanding the mechanism of the transition from epithelial to mesenchymal SCC cells.


Establishment and charcterization of chondrocyte cell lines from the costal cartilage of SV40 large T antigen transgenic mice

Complete understanding of the physiology and pathology of the cartilage is essential to establish treatments for a variety of cartilage disorders and defects such as rheumatoid arthritis, congenital malformations, and tumors of cartilage. Although synthetic materials have been used in many cases, they possess inherent problems including wear of the materials and low mechanical strength. Autograft has been considered very effective to overcome these problems. However, the limitation of the transplant volume is a major problem in autograft to be overcome. The costal cartilage is the most serious candidate for donor site transplantation, since it is the largest permanent hyaline cartilage in the body. To investigate the possibility using the costal cartilage as a transplant source, we have established and characterized three mouse chondrocyte cell lines (MCC-2, MCC-5, and MCC-35) derived from the costal cartilage of 8-week-old male SV40 large T-antigen transgenic mice. At confluence, all the cell lines formed nodules that could be positively stained with alcian blue (pH 2.5). The size of nodules gradually increased during culturing time. After 2 and 6 weeks of culture, RT-PCR analysis demonstrated that all three cell lines expressed mRNA from the cartilage-specific genes for type II collagen, type XI collagen, aggrecan, and link protein. Furthermore, type X collagen expression was detected in MCC-5 and MCC-35 but not in MCC-2. Any phenotypic changes were not observed over 31 cell divisions. Immunocytochemistry showed further that MCC-2, MCC-5, and MCC-35 produced cartilage-specific proteins type II collagen and type XI collagen, while in addition MCC-5 and MCC-35 produced type X collagen. Treatment with 1alpha, 25-dihydroxyvitamin D(3) inhibited cell proliferation and differentiation of the three cell lines in a dose-dependent manner. These phenotypic characteristics have been found consistent with chondrocyte cell lines established from cartilage tissues other than costal cartilage. In conclusion, costal cartilage shows phenotypic similarities to other cartilages, i.e., articular cartilage and embryonic limbs, suggesting that costal cartilage may be very useful as the donor transplantation site for the treatment of cartilage disorders. Furthermore, the cell lines established in this study are also beneficial in basic research of cartilage physiology and pathology.


A case of tetanus associated with trismus

開口障害を主訴として来院した破傷風の1例

Japanese Journal of Oral & Maxillofacial Surgery


A case of first and second branchial arch syndrome in a patient whose profile and occlusion improved in response to orthognatic surgery

顎矯正手術により顔貌および咬合の改善が得られた第1·第2鰓弓症候群の1例

We describe a case of first and second branchial arch syndrome in a patient whose profile and occlusion improved in response to orthognatic surgery.<BR>A 2-year-9-month-old boy was referred to our clinic for the treatment of open bite. Because symptoms were mild the patient was placed under observation. However, open bite and bird face became severer as he grew older. Cephalometric analysis indicated mandibular micrognathia. Before operation, we made and analyzed computer tomography-based three-dimensional models. The models were very useful for operation planning. After preoperative orthodontic treatment, sagittal splitting ramus osteotomy and horizontal sliding advancement genioplasty were performed in two stages.


国立療養所徳島病院における筋ジストロフィー患者の口腔内所見

国立療養所徳島病院における筋ジストロフィー患者の口腔内所見


徳島県立ひのみね整肢医療センター歯科における心身障害児(者)の診療実態について

徳島県立ひのみね整肢医療センター歯科における心身障害児(者)の診療実態について


Two cases of ameloblastic fibro-odontoma

エナメル上皮線維歯牙腫の2例


Effects of an anti-angiogenic agent, TNP-470, on the growth of oral squamous cell carcinomas


Expression of E-cadherin in oral cancer cell lines and its relationship to invasiveness in SCID mice in vivo


Histological and compositional evaluations of three types of calcium phosphate cements when implanted in subcutaneous tissue immediately after mixing

To evaluate the soft tissue response of calcium phosphate cement (CPC), consisting of an equimolar mixture of tetracalcium phosphate (TTCP) and dicalcium phosphate anhydrous (DCPA) under conditions close to those encountered in actual surgical procedures, we implanted three types of CPC [conventional CPC (c-CPC), fast-setting CPC (FSCPC), and antiwashout type FSCPC (aw-FSCPC; formerly called nondecay type FSCPC or nd-FSCPC)] subcutaneously in the abdomens of rats immediately (1 min) after mixing. At 1 week after surgery, histological examination and compositional analysis were performed using light microscopy and powder X-ray diffraction (XRD), respectively. The implanted c-CPC was crumbled completely, whereas FSCPC and aw-FSCPC retained their shape. Large vesicles containing copious inflammatory effusion were subcutaneously formed around the c-CPC. Histologically, many foreign-body giant cells were collected around the c-CPC, and moderate inflammatory cell infiltration was observed at 1 week after surgery. In contrast, the FSCPC and aw-FSCPC were covered with a thin layer of granulation tissue that included few giant cells and presented slight inflammatory cell infiltration, and no effusion was observed. The XRD analysis of the c-CPC revealed the presence of some unreacted DCPA even 1 week after implantation, whereas almost no DCPA was found in the FSCPC or aw-FSCPC. In conclusion, it was found that CPC does not always show excellent tissue response. When c-CPC is implanted subcutaneously in rats immediately after mixing, it fails to set and causes a severe inflammatory response. Therefore, the type of CPC should be chosen according to the clinical particulars. CPC should be used in a manner that assures its setting reaction. We recommend the use of FSCPC and aw-FSCPC for surgical applications, such as orthopedics, plastic and reconstructive surgery, and oral and maxillofacial surgery, where the cement might otherwise crumble due to the pressure before setting.

Journal of Biomedical Materials Research


Growth of normal oral keratinocytes and aquamous cell carcinoma cells in a novel protein-free defined medium


Initiation of ectopic epithelial calicification in a calcifying odontogenic cyst


Effects of added antibiotics on the basic properties of anti-washout-type fast-setting calcium phosphate cements


Three Cases of Application of Osseointegrated Implant to Surgical Reconstructive Area in Oral Cancer Patients --- Discussion of the Management of Reconstructive Soft Tissue Surrounding Implants

口腔癌切除後の外科的再建部へオッセオインテグレーテッドインプラントを応用した3例 --- インプラント周囲の外科的再建組織の処理法に関する考察

Journal of Japanese Society of Oral Implantology

日本口腔インプラント学会誌


ウサギ口腔粘膜における創傷治癒遅延モデルの作製

ウサギ口腔粘膜における創傷治癒遅延モデルの作製


Basic properties of calcium phosphate cement containing atelocollagen in its liquid or powder phases


Initial histological evaluation of anti-washout type fast-setting calcium phosphate cement following subcutaneous implantation


Non-decay type fast-setting calcium phosphate cement: Hydroxyapatite putty containing an increased amount of sodium alginate


Blast coating method: New method of coating titanium surface with hydroxyapatite at room temperature


The association of platelet-derived growth factor (PDGF) receptor tyrosine phosphorylation to mitogenic response of human osteoblastic cells in vitro


同一患者から樹立した3種の口腔癌細胞株とその性状

同一患者から樹立した3種の口腔癌細胞株とその性状

Japanese Journal of Oral & Maxillofacial Surgery


Tissue response to fast-setting calcium phosphate cement in bone


Carcinoma in Pleomorphic Adenoma of the Tongue ; Report of a case

A carcinoma in pleomorphic adenoma occurring in the tongue of a 47-year-old Japanese woman is reported. The present report is the first to describe a carcinoma in pleomorphic adenoma derived from the posterior lingual glands.


Verruciform xanthoma occurring within oral lichen planus


Non-decay type fast-setting calcium phosphate cement: setting behaviour in calf serum and its tissue response


Soft tissue response of calcium phosphate cements


Basic properties of hydroxyapatite putty made with fast-setting calcium phosphate cement and sodium alginate


Non-decay type fast-setting calcium phosphate cement using chitosan

Journal of Materials Science Materials in Medicine


Non-decay type fast-setting calcium phosphate cement --- : Composite with sodium alginate


Evaluation of serum concentration of parathyroid hormone-related protein and its implication in hypercalcemia in squamous cell carcinoma of the head and neck

Hypercalcemia is a common and serious complication associated with squamous cell carcinoma (SCC) and is considered to be caused by a tumor-derived factor, parathyroid hormone-related protein (PTHrP). However, the correlation between serum levels of calcium and PTHrP and the kinetics of PTHrP in SCC of the head and neck is unknown, because the behavior of the circulating form of PTHrP in patients has not been determined. In the present study, the PTHrP concentrations in serum samples from 54 patients (37 with SCC and 17 with benign tumors) were measured by a recently developed radioimmunoassay directed toward the C-terminal region of PTHrP, and the laboratory data including those calcium levels in corresponding samples were reviewed retrospectively. Results showed hypercalcemia in four patients with advanced cancer and in whom elevation of the serum PTHrP concentration was observed simultaneously. The regression analysis also revealed the linear relationship of the calcium level to the PTHrP concentration, but not to the concentration of phosphorus or creatinine, suggesting that monitoring of serum PTHrP level is useful for prediction of hypercalcemia associated with head and neck cancer.

International Journal of Oral and Maxillofacial Surgery


Simultaneous production of G-and M-CSF by an oral cancer cell line and the synergistic effects on associated leucocytosis

We established a novel cell line, TSU, from a oral cancer patient with marked leucocytosis. The culture supernatant of TSU cells promoted granulocytic colony formation by mouse bone marrow cells, indicating that TSU produced granulocyte-colony stimulating factor (G-CSF). The concentration of G-CSF was 2.45 micrograms/mg protein, measured by enzyme-linked immunosorbent assay (ELISA). The maximum number of colonies induced by TSU culture supernatant was more than that achieved with recombinant human G-CSF (rhG-CSF) and the size of the colonies induced by TSU supernatant was obviously larger than those achieved with rhG-CSF. The activity of TSU supernatant was completely inhibited by antihuman G-CSF and macrophage-colony stimulating factor (M-CSF) antibodies, but was only partially inhibited by antihuman G- or M-CSF antibody alone. These results indicate that not only G-CSF but also M-CSF, both of which could be produced by TSU cells, are involved in causing leucocytosis; the results suggest that the synergistic production of G- and M-CSF could play an important role in the leucocytosis associated with oral cancer.

European Journal of Cancer. Part B, Oral Oncology


Familial hyperparathyroidism associated with jaw fibroma: case report and literature review

A 53-year-old female suffering from renal stones and hypercalcaemia was diagnosed as having primary hyperparathyroidism caused by hyperplasia of the parathyroid glands. She underwent total parathyroidectomy and implantation of parathyroid tissue. After one year, she underwent surgery for a jaw tumour. The pathological findings indicated it to be a cementifying fibroma. Jackson et al. (1990) reported the familial association of hyperparathyroidism with jaw tumours, and they suggested that this condition represents a new clinical syndrome. We believe that our case belongs to this syndrome.


In vivo setting behavior of fast-setting calcium phosphate cement

The in vivo setting behaviour of fast-setting calcium phosphate cement (FSCPC) between femoral muscles of the rat was investigated to evaluate the possible value of FSCPC for medical and dental application. Conventional CPC (c-CPC) and FSCPC were implanted between femoral muscles, and various aspects of the setting behaviour such as setting time, mechanical strength and conversion ratio of cement into hydroxyapatite (HAP: Ca10(PO4)6(OH)2) were measured by the Vicat needle method, diametral tensile strength (DTS) measurement, and quantitative powder X-ray diffraction (XRD) analysis, respectively. The setting time of FSCPC in vivo was 5-7 min, in contrast to 48 min for c-CPC. As a result of its fast setting, set specimens of FSCPC showed higher mechanical strength from the initial stage than c-CPC. Higher DTS values were observed in FSCPC than c-CPC implanted after 24 h. Powder XRD analysis revealed faster conversion of FSCPC than c-CPC into HAP, which was responsible both for the faster setting and higher mechanical strength from the initial stage. We concluded, therefore, that FSCPC may be used for a wide range of clinical applications, i.e. fields where fast setting is required such as orthopaedic, plastic and reconstructive, and oral and maxillofacial surgery.


Diagnosis Significance of Scintigraphy in Head and Neck Cancer

頭頸部悪性腫瘍におけるシンチグラフィーの診断的意義

Journal of The Japanese Stomatological Society


Effects of Human EGF on Pulmonary Metastasis of Human Squamous Cell Carcinomas in SCID Mice

SCID マウスを用いた扁平上皮癌のin vivo 転移モデルにおけるヒトEGFの影響


Establiishiment and Characterization of Four Human Oral Cancer Cell Lines

4種のヒト口腔癌細胞株の樹立とその性状


A case of cystic cemento-ossifying fibroma in the mandible

下顎に生じた嚢胞性セメント質·骨形成線維腫の1例

Japanese Journal of Oral & Maxillofacial Surgery


過去10年間のガマ腫39症例の臨床的検討

過去10年間のガマ腫39症例の臨床的検討


Osteogenesis by human osteoblastic cells in diffusion chamber in vivo


維持透析患者に生じた舌癌の1治験例

維持透析患者に生じた舌癌の1治験例


Odontogenic cyst induction by periapical infection in rats


顎下腺と肺の重複癌の1例

顎下腺と肺の重複癌の1例


Effects of human EGF on the growth of human squamous cell carcinomas in athymic mice

ヌードマウス移植扁平上皮癌の増殖に対するヒトEGFの影響


セメント質形成線維腫の4例


Lung carcinoma suspected to metastarize to the submandibular region by the autopsy --- Report of a case

剖検により原発性肺癌の顎下部転移と考えられた1例 --- Report of a case


Behaviour of human ameloblastoma cells in collagen matrix in vitro: an ultrastructural study


Mineralized nodule formation in rat bone marrow stromal cell culture without beta-glycerophosphate


A Cleft Child With Lobulated Tongue and Lingual Hamartoma: Report of a Case

Journal of Oral and Maxillofacial Surgery


The behavior of human ameloblastoma tissue in collagen matrix in vitro:an immunohistochemical study


Solitary neurofibroma of the tongue: Report of a case

舌に孤立性に生じた神経線維腫の1例


Light-and Electron-Microscopic Observations on the Mandibular Condylar Cartilages in Growing Rats on a Low-Calcium Diet

In order to obtain more insight into the physiologic mechanism of endochondral ossification, histological changes occurring in the mandibular condylar cartilage of growing rats fed on a low-calcium diet were investigated by light and electron microscopy. Twenty-three-day-old rats were fed on a normal diet or a low-calcium diet for 8 weeks. For the histological observations the mandibular condyles were dissected from each animal at 1, 2, 4, 5 and 8 weeks after the initiation of the experiment. Histological changes occurring in the mandibular condylar cartilages of the rats fed on a low-calcium diet were as follows: (1) narrow proliferative and mature cell zones and a wide hypertrophic cell zone, (2) inhibition of development of cell organelles in the mature chondrocytes, (3) decrease in dead cells in the proliferative zone, (4) decrease in glycogen accumulation in the chondrocytes and (5) inhibition of calcification in the extracellular matrix of the hypertrophic cell zone. Additionally at the end of the experimental period, the following findings were observed: (1) appearance of small light cells in the mature cell zone and the hypertrophic cell zone and (2) decrease in proteoglycan granules and appearance of large collagen fibrils in the pericellular region of the hypertrophic cell zone.


当科における5年間の小児入院患者の臨床統計的観察

当科における5年間の小児入院患者の臨床統計的観察


Journal of The Japanese Stomatological Society


Ultrasturucture of mineralized nodules formed in rai bone marrow stromal cell culture in vitro

Rat bone marrow stromal cells were cultured in vitro. At days 14-15 of culture, dense clusters of polygonal cells were formed, and they mineralized 2-3 days later. The cells resembling osteoblasts or young osteocytes were histologically observed to be embedded in mineralized or unmineralized extracellular matrices of the nodules. Next, these mineralized nodules were electron-microscopically examined. The osteoblastic cells associated with the nodules had a well-developed rough endoplasmic reticulum, an evident Golgi apparatus and some mitochondria as their intracellular organellae. Some lysosomes and microfilaments were also visible in the cytoplasms. Moreover, some cells protruded cell processes toward the neighboring cells through the extracellular matrix. The extracellular matrix consisted of numerous collagen fibrils which were striated with 60-70 nm axial periodicity and which was similar to bone tissue collagen. A large number of matrix vesicles were scattered among the collagen fibrils in the unmineralized area of the nodules. In contrast, in the mineralized area, numerous matrix vesicles at different stages of maturation and many calcified spherules were observed. That is the mineralization in this culture system was considered to be initiated in association with the matrix vesicles and to progress along the collagen fibrils. From these findings, it was confirmed by the present study that the mineralized nodules formed in this bone marrow stromal cell culture were ultrastructurally similar to bone and that the mineralization also proceeded by going through the normal calcification process. This culture system is considered to be available to study osteogenic differentiation and calcification mechanisms.


Effect of retinoic acid on proliferation and differentiation of cultured chondrocytes in terminal differentiation


In vitro mineralization of osteoblastic cells derived from human bone

Osteoblastic cells were isolated from human maxilla by embedding the bone pieces in collagen gel. The isolated cells could be maintained in monolayer culture up to 50 population doubling levels (PDLs). Both parathyroid hormone (PTH) and prostaglandin E2 (PGE2) increased intracellular cyclic AMP level of the cells. The cells also showed high level of alkaline phosphatase (ALPase) activity and formed mineralized areas in monolayer culture. Electron microscopy demonstrated that these cells were surrounded by numerous well-banded collagen fibrils, among which matrix vesicles were scattered. It was also observed that needle-shaped crystals protruded from some matrix vesicles. These protruded crystals appeared to deposit along the collagen fibrils and a mineralized matrix was formed. The minerals of mineralized matrix mainly consisted of calcium and phosphorus and had the same Ca/P ratio as hydroxyapatite. These results indicate that the cells derived from human bone have characteristics of osteoblastic cells.


顎関節症における心因性要因の検討

顎関節症における心因性要因の検討


TMJ arthrosis related to psychological factors:Report of a case

心因性要因が大きいと思われた顎関節症患者の治療経験


Oblique facial clefts: A report of two cases


A case of gigantic schwannoma of the soft palate and pharynx

軟口蓋，咽頭に発現した巨大な神経鞘腫の1例


Japanese Journal of Oral & Maxillofacial Surgery


マウス(BALB/c)二次口蓋突起癒合に関する研究 --- 変性細胞の分布と上皮細胞の移動について


A case of radicular cyst arising from deciduous tooth

乳歯に生じた歯根嚢胞の1例


扁平上皮癌の免疫組織化学的研究 --- CEA, SC, IgAについて

扁平上皮癌の免疫組織化学的研究 --- CEA, SC, IgAについて


Epidermoid Cyst of the Uvula: Report of a Case

Journal of Oral and Maxillofacial Surgery


Clinical observations on 15 cases of dermoid (or epidermoid) cysts in the oral region

類皮·類表皮嚢胞15例の臨床的観察


Median cleft of the upper lip: Report of a case


血清中免疫抑制酸性蛋白，シアル酸およびC一反応性蛋白測定試薬の基礎的検討

血清中免疫抑制酸性蛋白，シアル酸およびC一反応性蛋白測定試薬の基礎的検討

徳島県臨床衛生検査技師会誌

徳島県臨床衛生検査技師会誌


Gene expression of telomerase related proteins in human normal oral and ectocervical epithelial cells

We analyzed telomerase activities and gene expressions of telomerase components: hTERT, hTR, hTEP1, telomeric repeat binding factors: TRF1, TRF2, and c-myc, Max and Mad in human normal oral and ectocervical epithelial keratinocytes, comparing with those of squamous carcinoma cells and HPV16- or SV40-immortalized cells. Significant telomerase activity and hTERT expression were detected in primary keratinocytes. However, both were dramatically down-regulated during serial passages. The down-regulation of hTERT mRNA was associated with augmented expression of TRF1. Expression of c-myc was slightly decreased, whereas Mad was expressed in parallel with that of hTERT during passages. We also detected an alternate splicing of hTERT transcript in two of four cancer cells and normal aged epithelial cells. These results suggest that the senescence of normal oral and ectocervical keratinocytes is accompanied with up-regulation of TRF1 and down-regulation of telomerase activity due to transcriptional suppression of active form of hTERT in vitro.


Hypercementosis with the progression of chronic apical periodontitis -A case report

慢性根尖性歯周炎の進行に伴って形成されたセメント質過形成症


口腔顎顔面手術におけるバイポーラシザーズの使用経験

口腔顎顔面手術におけるバイポーラシザーズの使用経験


Expression of E-cadherin in oralcancer cell lines and its relationship to invasiveness in SCID mice in vivo


Soft tissue responses of calcium phosphate cements


Basic properties of hydroxyapatite putty made with fast-setting calcium phosphate cement and sodium alginate


Approaches for bone-replacing calcium phosphate cement --- Effect of NaHCO3 addition on the properties of calcium phosphate cement