read0046145/published_papers/54167238 Daisuke Nakamura Yuji Inagaki Yuta Uemura Yuka Hiroshima Yoshimune Iwata Rie Kido Mika Bando Tomoko Sumitomo Hiromichi Yumoto Porphyromonas gingivalis outer membrane vesicles promote alveolar bone resorption by increasing the local RANKL/OPG ratio in experimental periodontitis model rats Springer Science and Business Media LLC Scientific Reports 2045-2322 16 1 null null 20260405 10.1038/s41598-026-46625-4 read0046145/published_papers/52874945 Yukari Hatada Yuji Inagaki Daisuke Nakamura Minato Akizuki Yoshimune Iwata Rie Kido Mika Bando Hiromichi Yumoto Tryptanthrin, a constituent of Persicaria tinctoria, suppresses osteoclastogenesis and enhances the expression of tight junction proteins and cell adhesion molecules in gingival epithelial cells. Tryptanthrin, an ingredient of Persicaria tinctoria, has been reported to exhibit antioxidant and anti-inflammatory effects. To elucidate the potential role of tryptanthrin in periodontal disease, we investigated its effects on bone metabolism and innate immunity, including the epithelial barrier. RAW264.7 cells were cultured with soluble receptor activator of nuclear factor-κB ligand (sRANKL) and tryptanthrin, and stained with Tartrate-Resistant Acid Phosphatase (TRAP) to evaluate osteoclastgenesis. The expression of osteoclast differentiation markers was examined using western blot analysis. OBA-9 cells were cultured with tryptanthrin, and the expression of tight junction (TJ) proteins and cell adhesion molecules (CAMs) was examined by western blot analysis. We found that tryptanthrin significantly inhibited osteoclastgenesis and suppressed the expression of osteoclast differentiation markers. Further, tryptanthrin enhanced the expression of TJ proteins and CAMs. These results indicated that tryptanthrin inhibits osteoclastgenesis by downregulating the osteoclast differentiation markers and enhances cell-cell adhesion by upregulating TJ proteins and CAMs. Bioscience, biotechnology, and biochemistry null null 20260210 10.1093/bbb/zbag025 41665875 read0046145/published_papers/52874952 Jun-Ichi Kido Rie Kido Sachiko Ando Atsuko Shinozaki Mika Bando Yuji Inagaki Yasuhiro Seki Yoshiyuki Ohiro Hiromichi Yumoto Evaluation of salivary calprotectin as a marker for screening periodontitis using a latex agglutination turbidimetric immunoassay system: a cross-sectional study. BACKGROUND: Biomolecules in body fluids such as gingival crevicular fluid and saliva are used for the diagnosis of periodontitis. Saliva is easy to collect and salivary biomarkers are useful in screening periodontitis. The suitable salivary biomarkers and their measuring system are important for screening periodontitis in mass dental examination. Therefore, this study examined the potential of few salivary biomarkers for screening and diagnosis of periodontitis and aimed to evaluate more effective biomarkers and their measuring methods in dental examination. METHODS: Ninety-three individuals with and without periodontitis participated in this clinical examination and were classified into the non-periodontal diseases or stage I periodontitis (control, n = 26) and stage II-IV periodontitis groups (periodontitis, n = 67) after periodontal examinations. Unstimulated saliva samples were collected from participants. The levels of salivary biomarkers including calprotectin, hemoglobin (Hb), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), alanine aminotransferase and aspartate aminotransferase (AST) were automatically measured using the latex agglutination turbidimetric immunoassay (LATIA) and enzyme assay systems. Differences in clinical indicator and biomarker levels in the control and periodontitis groups and their correlations were statistically analyzed. A receiver operating characteristic (ROC) analysis of the ability of salivary biomarkers to predict periodontitis was also performed. RESULTS: Salivary calprotectin, Hb, LDH, ALP and AST levels were significantly higher in the periodontitis group than that in the control group. At the initial stage of periodontitis, a significant difference was only observed in calprotectin levels. Calprotectin and LDH levels strongly correlated with clinical indicators including probing pocket depth, clinical attachment level, bleeding on probing, gingival index and periodontal inflamed surface area with high correlation coefficient (calprotectin: 0.582-0.660, LDH: 0.534-0.614). Calprotectin showed a higher area under the ROC curve value (0.894), with 91% sensitivity and 73% specificity, than the other salivary biomarkers. CONCLUSIONS: Salivary calprotectin showed a high effectiveness for the diagnosis of periodontitis, and the measurement of salivary calprotectin using the LATIA system that is a high throughput method is suitable for population-based screening of periodontal diseases. BMC oral health 26 1 240 240 20260107 10.1186/s12903-025-07622-7 41501804 read0046145/published_papers/52515256 Raras Ajeng Enggardipta Minato Akizuki Mika Bando Yuji Inagaki Kazumitsu Sekine Kenichi Hamada Tomoko Sumitomo Kanta Sato Hiromichi Yumoto Trimethyl chitosan: Antibacterial activity on Enterococcus faecalis biofilm and cytocompatibility on human periodontal ligament fibroblasts cells Elsevier BV Journal of Dental Sciences 1991-7902 null null 20250700 10.1016/j.jds.2025.06.022 read0046145/published_papers/52874955 Rie Kido Yuka Hiroshima Jun-Ichi Kido Mika Bando Kaya Yoshida Yuji Inagaki Hiromichi Yumoto Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells. OBJECTIVES: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases. METHODS: The presence of 24p3R was examined in periodontal cells, including human gingival fibroblasts, periodontal ligament fibroblasts, human oral epithelial cells (HOECs), and neutrophil-like cells (HL-60), by Western blotting. Changes in periodontal disease-associated proteins in the presence of recombinant LCN2 (rLCN2) were examined using a protein array in differentiated HL-60 (dHL-60) cells. Interleukin-8 (IL-8) and macrophage inflammatory protein-1α (MIP-1α) mRNA expressions were analyzed by qRT-PCR, and IL-8 and MIP-1α levels in dHL-60 cells treated with rLCN2 or Porphyromonas gingivalis-lipopolysaccaharide (P.g-LPS) were determined by enzyme-linked immunosorbent assay. RESULTS: We detected 24p3R in dHL-60 cells. IL-8 was highly expressed and MIP-1α was weakly expressed in dHL-60 cells using a protein array. rLCN2 significantly decreased IL-8 mRNA and protein levels and suppressed P.g-LPS-induced IL-8 production in dHL-60 cells. As dHL-60 cells were co-cultured with HOECs in which LCN2 was knocked down, IL-8 mRNA expression increased in dHL-60 cells. Furthermore, rLCN2 inhibited MIP-1α production in dHL-60 cells. CONCLUSION: LCN2 suppresses inflammatory responses by regulating IL-8 and MIP-1α expression in periodontal diseases. Journal of oral biosciences 67 1 100624 100624 20250300 10.1016/j.job.2025.100624 39892784 read0046145/published_papers/49860863 Minato Akizuki Keiji Murakami Kazumitsu Sekine Akikazu Murakami Koh Kobayashi Masaru Matsuda Haruka Matsumoto Eiji Harata Kenichi Hamada Raras Ajeng Enggardipta Hideki Fujii Hiromichi Yumoto Hydrophobic 2-methacryloyloxyethyl phosphorylcholine polymer inhibits peri-implantitis-causing bacterial adhesion on titanium materials Abstract Aims To prevent peri-implantitis, we investigated the adhesion of periodontopathogenic bacteria to titanium surfaces using a hydrophobic 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer to inhibit adhesion. Method and results We immersed titanium plates (TiPs) coated with a hydrophobic MPC polymer in a bacterial suspension for 30 min or 24 h and measured the number of adherent bacteria. Bacteria adhering to the TiPs were observed using scanning electron microscopy (SEM). Furthermore, mimicking an oral cavity, TiPs coated with MPC polymer and saliva, were immersed in bacterial suspensions of Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans for 24 h, and adenosine triphosphate in the adherent bacteria was measured. Bacterial adhesion was significantly inhibited on MPC polymer-coated TiPs after 30 min and 24 h. SEM results showed a similar trend. Bacterial adhesion was significantly inhibited on MPC polymer-treated TiPs in the presence of saliva, both before and after MPC treatment. Furthermore, their effectiveness was maintained when the MPC polymer-treated TiPs were stored in saline for 1 week. Conclusions Hydrophobic MPC polymer coating on TiP surface inhibited bacterial adhesion, indicating that it may be effective in preventing peri-implantitis. Oxford University Press (OUP) Journal of Applied Microbiology 1365-2672 136 2 null null 20250203 10.1093/jambio/lxaf033 read0046145/published_papers/48937464 Hitomi Kuramoto Tadashi Nakanishi Hiromichi Yumoto Daisuke Takegawa Katsuhiro Mieda Keiichi Hosaka Caffeic Acid Phenethyl Ester Enhances Bone Repair-related Factors in MC3T3-E1 Cells. Apical periodontitis is an inflammatory disease caused by bacterial infection in the root canal that spreads to the apical periodontal tissues, resulting in bone resorption around the root apex as the disease progresses. Vascular endothelial growth factor (VEGF), a growth factor involved in angiogenesis, plays an important role in bone remodeling. We reported that caffeic acid phenethyl ester (CAPE), a bioactive substance of propolis, induces VEGF in odontoblast-like cells and dental pulp cells. However, the effects of CAPE on bone tissues remain unclear. This study was aimed to investigate the effects of CAPE on MC3T3-E1 cells, mice preosteoblast line. As a result, CAPE up-regulated the production of VEGF and induced the phosphorylation of extracellular signal-regulated kinases (ERK), p38 mitogen-activated protein kinase (MAPK), and stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) in MC3T3-E1 cells. Furthermore, CAPE increased the expression of factors involved in osteoblast differentiation, runt-related transcription factor 2 (Runx2), Osterix, and Wnt5a/b in MC3T3-E1 cells. In this study, we show that CAPE could induce bone repair-related factors in MC3T3-E1 cells. Cell biochemistry and biophysics null null 20241221 10.1007/s12013-024-01644-8 39708213 read0046145/published_papers/48475603 Kenji Masutomi Mika Bando Yuji Inagaki Rie Kido Yuta Uemura Yukari Hatada Jun Ichi Kido Makoto Fukui Daisuke Hinode Hiromichi Yumoto Relationship between oral hypofunction and salivary biomarkers in older adults: a cross-sectional study BMC Oral Health 1472-6831 24 1 null null 20241200 10.1186/s12903-024-04556-4 38971720 2-s2.0-85197697204 read0046145/published_papers/41825080 Jun-Ichi Kido Yuka Hiroshima Rie Kido Kaya Yoshida Yuji Inagaki Koji Naruishi Kazuaki Kajimoto Masatoshi Kataoka Yasuo Shinohara Hiromichi Yumoto Lipocalin 2, synthesized using a cell-free protein synthesis system and encapsulated into liposomes, inhibits the adhesion of Porphyromonas gingivalis to human oral epithelial cells. BACKGROUND AND OBJECTIVE: Lipocalin 2 (LCN2), a glycoprotein expressed in epithelial cells and leukocytes, has an antibacterial effect and plays a role in innate immunity. The delivery of LCN2 encapsulated in liposomes to oral epithelium may be useful to prevent oral infectious diseases. This study aimed to investigate the inhibitory effect of LCN2, artificially synthesized using a cell-free protein synthesis (CFPS) system, on the adhesion of Porphyromonas gingivalis to oral epithelial cells in order to approach oral healthcare using LCN2. METHODS: LCN 2 was synthesized using a CFPS system and assayed by Western blotting, mass spectrometry and enzyme-linked immunosorbent assay (ELISA). The bilayer liposomes were prepared by the spontaneous transfer method using 1,2-dioleoyl-sn-glycero-3 phosphocholine (DOPC), 3-sn-phosphatidylcholine from Egg Yolk (Egg-PC), and 1,2-dioleoyl-sn-glycero-3 phosphoethanolamine (DOPE). The cellular and medium fractions derived from the culture of oral epithelial cells with liposome-encapsulated LCN2 were assayed by Western blotting and ELISA. The effect of the synthesized LCN2 on adhesion of the labeled P. gingivalis to oral epithelial cells was investigated as an evaluation of its antibacterial activity. RESULTS: The synthesized LCN2 protein was identified by Western blotting; its amino acid sequence was similar to that of recombinant LCN2 protein. The additions of DOPE and octa-arginine in the outer lipid-layer components of liposome significantly increased the delivery of liposomes to epithelial cells. When oral epithelial cells were cultured with the synthesized and liposome-encapsulated LCN2, LCN2 was identified in the cellular and medium fractions by Western blotting and its concentration in the cellular fraction from the culture with the synthesized LCN2 was significantly higher than that of a template DNA-free protein. The synthesized LCN2 and liposome-encapsulated LCN2 significantly inhibited the adhesion of P. gingivalis to oral epithelial cells compared with template DNA-free protein. CONCLUSION: LCN2 was artificially synthesized by a CFPS system, encapsulated in liposomes, and delivered to oral epithelial cells, and demonstrated an antibacterial action against P. gingivalis. This approach may become a useful model for oral healthcare. Journal of periodontal research 58 2 262 273 20230400 10.1111/jre.13088 36579753 read0046145/published_papers/41393811 Yasufumi Nishikawa Yoritoki Tomotake Hiromichi Kawano Koji Naruishi Jun-ichi Kido Yuka Hiroshima Akikazu Murakami Tetsuo Ichikawa Hiromichi Yumoto Effects of Candidalysin Derived from Candida albicans on the Expression of Pro-Inflammatory Mediators in Human Gingival Fibroblasts Candida albicans (Ca) is frequently detected in the peri-implant sulcus with peri-implantitis, a major postoperative complication after oral implant therapy. However, the involvement of Ca in the pathogenesis of peri-implantitis remains unclear. In this study, we aimed to clarify Ca prevalence in the peri-implant sulcus and investigated the effects of candidalysin (Clys), a toxin produced by Ca, on human gingival fibroblasts (HGFs). Peri-implant crevicular fluid (PICF) was cultured using CHROMagar and Ca colonization rate and colony numbers were calculated. The levels of interleukin (IL)-1β and soluble IL-6 receptor (sIL-6R) in PICF were quantified by enzyme-linked immunosorbent assay (ELISA). Pro-inflammatory mediator production and intracellular signaling pathway (MAPK) activation in HGFs were measured by ELISA and Western blotting, respectively. The Ca colonization rate and the average number of colonies in the peri-implantitis group tended to be higher than those in the healthy group. IL-1β and sIL-6R levels in the PICF were significantly higher in the peri-implantitis group than in the healthy group. Clys significantly induced IL-6 and pro-matrix metalloproteinase (MMP)-1 productions in HGFs, and co-stimulation with Clys and sIL-6R increased IL-6, pro-MMP-1, and IL-8 production levels in HGFs compared with Clys stimulation alone. These findings suggest that Clys from Ca plays a role in the pathogenesis of peri-implantitis by inducing pro-inflammatory mediators. MDPI AG International Journal of Molecular Sciences 1422-0067 24 4 3256 3256 20230207 10.3390/ijms24043256 read0046145/published_papers/41825076 Yuka Hiroshima Jun-Ichi Kido Rie Kido Kaya Yoshida Mika Bando Kazuaki Kajimoto Hiromichi Yumoto Yasuo Shinohara β-defensin 2 synthesized by a cell-free protein synthesis system and encapsulated in liposomes inhibits adhesion of Porphyromonas gingivalis to oral epithelial cells. β-defensin 2 (BD-2), an antimicrobial peptide (AMP), is expressed by oral epithelial cells and plays an important role in innate immunity of the oral cavity. Cell-free protein synthesis (CFPS) systems have been studied for the synthesis of various proteins, however, the synthesis of BD-2 by a CFPS system has not been extensively explored. Liposomes have been developed as tools for drug delivery. A delivery of liposome-encapsulated AMP to oral epithelium may be useful to prevent oral infectious diseases. In the present study, we investigated the antimicrobial activity of the BD-2 protein, artificially synthesized using a CFPS system and encapsulated in liposomes. BD-2 protein was artificially synthesized using template DNA and a reconstituted CFPS system and was identified by western blotting. Bilayer liposomes were prepared using 1,2-dioleoyl-sn-glycero-3-phospho-choline and 3-sn-phosphatidylcholine from egg yolk. The artificially synthesized BD-2 was encapsulated in liposomes, collected by ultrafiltration, and detected by western blotting. Human oral epithelial cells were cultured with the liposome-encapsulated BD-2 and the concentration of BD-2 in the cell lysate of the culture with the synthesized BD-2 was higher than that of the control cultures. The antimicrobial activity of the synthesized BD-2 was investigated by an adhesion assay of Porphyromonas gingivalis to oral epithelial cells. The artificially synthesized BD-2 and its liposome significantly inhibited adhesion of P. gingivalis to oral epithelial cells. These results suggest that artificially synthesized BD-2 and liposome-encapsulated BD-2 show antimicrobial activity and can potentially play a role in oral healthcare for periodontal diseases. Odontology null null 20230206 10.1007/s10266-023-00789-x 36745267 read0046145/published_papers/40746395 Yuta Uemura Yuka Hiroshima Ayano Tada Keiji Murakami Kaya Yoshida Yuji Inagaki Tomomi Kuwahara Akikazu Murakami Hideki Fujii Hiromichi Yumoto Porphyromonas gingivalis Outer Membrane Vesicles Stimulate Gingival Epithelial Cells to Induce Pro-Inflammatory Cytokines via the MAPK and STING Pathways Porphyromonas gingivalis (Pg) is a keystone pathogen associated with chronic periodontitis and produces outer membrane vesicles (OMVs) that contain lipopolysaccharide (LPS), gingipains, and pathogen-derived DNA and RNA. Pg-OMVs are involved in the pathogenesis of periodontitis. Pg-OMV-activated pathways that induce the production of the pro-inflammatory cytokines, interleukin (IL)-6, and IL-8 in the human gingival epithelial cell line, OBA-9, were investigated. The role of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB in levels of Pg-OMV-induced pro-inflammatory cytokines was investigated using Western blot analysis and specific pathway inhibitors. Pg-OMVs induced IL-6 and IL-8 production via the extracellular signal-regulated kinase (Erk) 1/2, c-Jun N-terminal kinase (JNK), p38 MAPK, and NF-κB signaling pathways in OBA-9 cells. In addition, the stimulator of interferon genes (STING), an essential innate immune signaling molecule, was triggered by a cytosolic pathogen DNA. Pg-OMV-induced IL-6 and IL-8 mRNA expression and production were significantly suppressed by STING-specific small interfering RNA. Taken together, these results demonstrated that Pg-OMV-activated Erk1/2, JNK, p38 MAPK, STING, and NF-κB signaling pathways resulting in increased IL-6 and IL-8 expression in human gingival epithelial cells. These results suggest that Pg-OMVs may play important roles in periodontitis exacerbation by stimulating various pathways. MDPI AG Biomedicines 2227-9059 10 10 2643 2643 20221020 10.3390/biomedicines10102643 read0046145/published_papers/46782942 Akihiro Wada Keiji Murakami Yumi Ishikawa Takashi Amoh Kouji Hirao Yuki Hosokawa Daisuke Hinode Yoichiro Miyake Hiromichi Yumoto Anti-Inflammatory and Protective Effects of Juncus effusus L. Water Extract on Oral Keratinocytes BioMed Research International 2314-6141 2022 null null 20220000 10.1155/2022/9770899 35028318 2-s2.0-85123567270 read0046145/published_papers/46782940 Akihiro Wada Keiji Murakami Yumi Ishikawa Takashi Amoh Kouji Hirao Yuki Hosokawa Daisuke Hinode Yoichiro Miyake Hiromichi Yumoto Anti-Inflammatory and Protective Effects of Juncus effusus L. Water Extract on Oral Keratinocytes. Periodontitis is a chronic inflammatory disease caused by periodontopathogenic bacteria that form biofilms in periodontal pockets. The gingival epithelium acts as the first physical barrier in fighting attacks by periodontopathogenic pathogens, such as the primary etiological agent Porphyromonas gingivalis, and various exogenous chemicals, as well as regulates the local innate immune responses. Therefore, the development of novel oral care products to inhibit inflammatory reactions caused by bacterial infection and protect the gingival epithelium is necessary. Juncus effusus L. has generally been used as an indigenous medicine, such as a diuretic, an antipyretic, and an analgesic, in ancient practice. In this study, we examined the effects of a water extract from J. effusus L. on the inhibition of the inflammatory reaction elicited by bacterial infection and protection of the oral epithelium by chemical irritation. Pretreatment of oral epithelial cells with the water extract from J. effusus L. significantly reduced P. gingivalis or its lipopolysaccharide- (LPS-) mediated production of chemokines (interleukin-8 and C-C-chemokine ligand20) in a concentration-dependent manner with comparable to or greater effects than epigallocatechin gallate and protected oral epithelial cells from injury by chemical irritants, cetylpyridinium chloride, and benzethonium chloride. Moreover, the water extract from J. effusus L. in the presence of antimicrobial agents or antifibrinolytics already used as ingredients in mouthwash could significantly reduce the production of chemokines from P. gingivalis LPS-stimulated oral epithelial cells in a concentration-dependent manner. These findings suggest that the water extract from J. effusus L. is potentially useful for oral care to prevent oral infections, such as periodontal infections, and maintain oral epithelial function. BioMed research international 2022 9770899 9770899 20220000 10.1155/2022/9770899 35028318 read0046145/published_papers/34931341 Wenhua Shao Natsumi Fujiwara Yasuhiro Mouri Satoru Kisoda Kayo Yoshida Kaya Yoshida Hiromichi Yumoto Kazumi Ozaki Naozumi Ishimaru Yasusei Kudo Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells <title>Abstract</title> The ability of cancer cells to undergo partial-epithelial mesenchymal transition (p-EMT), rather than complete EMT, poses a higher metastatic risk. Although <italic>Fusobacterium nucleatum</italic> mainly inhabits in oral cavity, attention has been focused on the <italic>F. nucleatum</italic> involvement in colorectal cancer development. Here we examined the p-EMT regulation by <italic>F. nucleatum</italic> in oral squamous cell carcinoma (OSCC) cells. We cultured OSCC cells with epithelial, p-EMT or EMT phenotype with live or heat-inactivated <italic>F. nucleatum</italic>. Expression of the genes involved in epithelial differentiation, p-EMT and EMT were examined in OSCC cells after co-culture with <italic>F. nucleatum</italic> by qPCR. Cell growth and invasion of OSCC cells were also examined. Both live and heat-inactivated <italic>F. nucleatum</italic> upregulated the expression of p-EMT-related genes in OSCC cells with epithelial phenotype, but not with p-EMT or EMT phenotype. Moreover, <italic>F. nucleatum</italic> promoted invasion of OSCC cells with epithelial phenotype. Co-culture with other strains of bacteria other than <italic>Porphyromonas gingivalis</italic> did not alter p-EMT-related genes in OSCC cells with epithelial phenotype. <italic>F. nucleatum</italic> infection may convert epithelial to p-EMT phenotype via altering gene expression in OSCC. Oral hygiene managements against <italic>F. nucleatum</italic> infection may contribute to reduce the risk for an increase in metastatic ability of OSCC. Springer Science and Business Media LLC Scientific Reports 2045-2322 11 1 null null 20211200 10.1038/s41598-021-94384-1 read0046145/published_papers/35184923 Rie Kido Jun-Ichi Kido Yasufumi Nishikawa Eijiro Sakamoto Yoritoki Tomotake Hiromichi Yumoto Diagnosis of inflammatory peri-implant diseases using an immunochromatographic assay for calprotectin in peri-implant crevicular fluid. BACKGROUND: The incidence rate of peri-implant diseases is increasing with implant placement. Early detection of peri-implant diseases is important to prevent and treat these diseases, and a simple and objective diagnostic method is expected. Immunochromatographic (IC) assays are used for rapid diagnostic methods for some diseases. The aim of this clinical study was to determine the amount of calprotectin, an inflammatory marker, in peri-implant crevicular fluid (PICF) using an IC chip, and estimate the possibility of this diagnostic system. METHODS: Forty-six individuals with dental implants participated in a pilot study. PICF samples were collected from the peri-implant sites with or without inflammation after clinical examinations including probing depth (PD), bleeding on probing (BOP) and gingival index (GI). Calprotectin in PICF was determined by an IC chip and enzyme-linked immunosorbent assay (ELISA) for calprotectin. The density of calprotectin line on the IC chip was measured using an IC reader (IC reader value). The relationship between IC reader value and ELISA value or clinical parameters was investigated. A receiver operating characteristic (ROC) curve analysis of IC reader value of calprotectin was performed to predict inflammation in peri-implant diseases. RESULTS: IC reader value of calprotectin was significantly correlated with its ELISA value and PD. IC reader values of calprotectin in PICF samples from periodontal sites with GI-1 and GI-2, and with BOP-positive sites were significantly higher than those of PICF samples from GI-0 sites, and BOP-negative sites, respectively. The IC reader value for calprotectin in PICF samples from inflammatory diseased sites was significantly higher than that of non-diseased sites. ROC analysis suggested that the IC reader value of PICF calprotectin was useful for predicting inflammatory peri-implant diseases. CONCLUSION: IC assay for PICF calprotectin may be a possible system for diagnosing the inflammatory peri-implant diseases. International journal of implant dentistry 7 1 106 106 20211008 10.1186/s40729-021-00386-z 34623545 read0046145/published_papers/39576865 生田 貴久 二宮 雅美 秋月 皆人 植村 勇太 成石 浩司 湯本 浩通 PCRを用いたPorphyromonas gingivalis 2型線毛迅速検出システムの有効性の検討 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 63 99 99 20210500 read0046145/published_papers/35026304 西川 泰史 二宮 雅美 川野 弘道 木戸 理恵 友竹 偉則 湯本 浩通 限局型侵襲性歯周炎患者に対してリグロスとサイトランスグラニュールを併用した歯周組織再生療法とインプラント治療を行った1症例 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 63 119 119 20210500 read0046145/published_papers/42924813 弘田 克彦 米山 武義 湯本 浩通 大野 由香 中石 裕子 野村 加代 坂本 まゆみ 和食 沙紀 濱田 美晴 内田 智子 歯周病およびNETsとがんを含む全身疾患 全国大学歯科衛生士教育協議会編集委員会 全国大学歯科衛生士教育協議会雑誌 2186-8638 10 19 27 20210300 read0046145/published_papers/41581622 湯本 浩通 弘田 克彦 三宅 洋一郎 村上 圭史 藤原 奈津美 秋月 皆人 藤猪 英樹 "撥菌" 口腔バイオフィルム感染症予防への応用 口腔バイオフィルム感染症の予防には，細菌の付着を抑制することが効果的な方策であると考えられる．近年，細菌を付着させにくくする"撥菌"という概念が提唱された．この"撥菌材"について，材料表面をコーティングすることにより，その性状を超親水性に改変する2-methacryloyloxyethyl phosphorylcholine(MPC)-polymerが開発された．筆者らが行ってきたMPC-polymerのオーラルケアへの応用に関する研究を紹介した． 全国大学歯科衛生士教育協議会編集委員会 全国大学歯科衛生士教育協議会雑誌 2186-8638 10 13 17 20210300 read0046145/published_papers/35026208 Yuji Inagaki Jun-Ichi Kido Yasufumi Nishikawa Rie Kido Eijiro Sakamoto Mika Bando Koji Naruishi Toshihiko Nagata Hiromichi Yumoto Gan-Lu-Yin (Kanroin), Traditional Chinese Herbal Extracts, Reduces Osteoclast Differentiation In Vitro and Prevents Alveolar Bone Resorption in Rat Experimental Periodontitis Gan-Lu-Yin (GLY), a traditional Chinese herbal medicine, shows therapeutic effects on periodontitis, but that mechanism is not well known. This study aims to clarify the precise mechanism by investigating the inhibitory effects of GLY extracts on osteoclastogenesis in vitro and on bone resorption in periodontitis in vivo. RAW264.7 cells are cultured with soluble receptor activator of nuclear factor-kappa B (sRANKL) and GLY extracts (0.01-1.0 mg/mL), and stained for tartrate-resistant acid phosphatase (TRAP) to evaluate osteoclast differentiation. Experimental periodontitis is induced by placing a nylon ligature around the second maxillary molar in rats, and rats are administered GLY extracts (60 mg/kg) daily for 20 days. Their maxillae are collected on day 4 and 20, and the levels of alveolar bone resorption and osteoclast differentiation are estimated using micro-computed tomography (CT) and histological analysis, respectively. In RAW264.7 cells, GLY extracts significantly inhibit sRANKL-induced osteoclast differentiation at a concentration of more than 0.05 mg/mL. In experimental periodontitis, administering GLY extracts significantly decreases the number of TRAP-positive osteoclasts in the alveolar bone on day 4, and significantly inhibits the ligature-induced bone resorption on day 20. These results show that GLY extracts suppress bone resorption by inhibiting osteoclast differentiation in experimental periodontitis, suggesting that GLY extracts are potentially useful for oral care in periodontitis. Journal of clinical medicine 10 3 null null 20210120 10.3390/jcm10030386 33498415 read0046145/published_papers/34850613 Eijiro Sakamoto Yuka Hiroshima Jun-ichi Kido Yasufumi Nishikawa Koji Naruishi Rie Kido Hiromichi Yumoto Various roles of calprotectin in periodontal disease and its possibility as a diagnostic marker for periodontal disease Japanese Society of Periodontology Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) 1880-408X 62 4 193 199 20201228 10.2329/perio.62.193 read0046145/published_papers/31372015 西川泰史 成石浩司 木戸淳一 湯本浩通 高グルコース条件下における歯肉線維芽細胞のカルプロテクチン誘導性炎症関連因子の産生におけるスダチチンの抑制効果 日本歯科保存学雑誌 63 6 503 511 20201200 read0046145/published_papers/41581624 植村 勇太 廣島 佑香 稲垣 裕司 湯本 浩通 多田 彩乃 村上 圭史 藤猪 英樹 Porphyromonas gingivalis由来メンブレンベシクルがヒト歯肉上皮細胞に及ぼす影響 (NPO)日本歯科保存学会 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 153 98 98 20201100 read0046145/published_papers/39576868 秋月 皆人 二宮 雅美 植村 勇太 生田 貴久 成石 浩司 湯本 浩通 PCRを用いたPorphyromonas gingivalis迅速検出システムの有用性 (NPO)日本歯科保存学会 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 153 97 97 20201100 read0046145/published_papers/39576870 二宮 雅美 植村 勇太 和田 明大 生田 貴久 湯本 浩通 下顎第一大臼歯の重度骨欠損に対してリグロスとサイトランスグラニュールを併用した歯周組織再生療法を行った一症例 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 62 134 134 20201000 read0046145/published_papers/29486972 Natsumi Fujiwara Keiji Murakami Kaya Yoshida Shunsuke Sakurai Yasusei Kudo Kazumi Ozaki Katsuhiko Hirota Hideki Fujii Maiko Suzuki Yoichiro Miyake Hiromichi Yumoto Suppressive effects of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer on the adherence of Candida species and MRSA to acrylic denture resin. Objectives: The effects of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer on the adherence of microorganisms such as non-Candida albicans Candida (NCAC) and methicillin-resistant Staphylococcus aureus (MRSA), frequently detected in oral infections in immunocompromised and/or elderly people, to denture resin material, are still unclear. Here, we report the effects of MPC-polymer on the adherence of C. albicans, NCAC, and MRSA to acrylic denture resin. Methods: Sixteen strains of C. albicans, seven strains of C. glabrata, two strains of C. tropicalis, one strain of C. parapsilosis, and six strains of MRSA were used. We cultured the fungal/bacterial strains and examined the cell growth and adherence of fungi/bacteria to mucin-coated acrylic denture resin plates (ADRP) with or without MPC-polymer coating, by scanning electron microscopy. The cell surface hydrophobicity of the fungal/bacterial strains was measured by the adsorption to hydrocarbons. Results: MPC-polymer did not affect the growth of all strains of Candida species and MRSA, but significantly suppressed adherence to ADRP in most strains of C. albicans and all strains of NCAC and MRSA. A significant positive correlation was found between cell hydrophobicity and the reduction rates of microbial adherence to ADRP treated with 5% of MPC-polymer. Conclusions: MPC-polymer treatment for acrylic resin material suppresses the adherence of C. albicans, NCAC and MRSA via their hydrophilicity interaction. Clinical significance: The application of MPC-polymer for denture hygiene is potent to prevent oral candidiasis, denture stomatitis and opportunistic infection, caused by Candida species and MRSA, via suppressing the adherence of those fungus/bacteria. Heliyon 6 6 e04211 null 20200600 10.1016/j.heliyon.2020.e04211 32577575 read0046145/published_papers/31371995 稲垣 幸司 菊池 毅 野口 俊英 三谷 章雄 成瀬 桂子 松原 達昭 川浪 雅光 根岸 淳 古市 保志 根本 英二 山田 聡 吉江 弘正 多部田 康一 富田 幸代 齋藤 淳 片桐 さやか 和泉 雄一 新田 浩 岩田 隆紀 沼部 幸博 山本 松男 吉成 伸夫 藤田 剛 栗原 英見 西村 英紀 永田 俊彦 湯本 浩通 内藤 徹 野口 和行 伊藤 公一 村上 伸也 西村 理明 田嶼 尚子 糖尿病データベース構築委員会 糖尿病合併症の実態とその抑制に関する大規模観察研究ベースライン時の口腔所見JDCP study 6 (一社)日本糖尿病学会 糖尿病 1881-588X 63 4 195 205 20200000 read0046145/published_papers/31371960 Saori Matsui Naomichi Yoneda Hazuki Maezono Katsutaka Kuremoto Takuya Ishimoto Takayoshi Nakano Hiromichi Yumoto Shigeyuki Ebisu Yuichiro Noiri Mikako Hayashi Assessment of the functional efficacy of root canal treatment with high frequency waves in rats The purpose of this study was to develop a high-frequency wave therapy model in rats and to investigate the influence of high-frequency waves on root canal treatment, which may provide a novel strategy for treating apical periodontitis. Root canal treatments with and without high-frequency wave irradiation were performed on the mandibular first molars of 10-week-old male Wistar rats. The mesial roots were evaluated radiologically, bacteriologically, and immunohistochemically. At 3 weeks after root canal treatment, lesion volume had decreased significantly more in the irradiated group than in the non-irradiated group, indicating successful development of the high-frequency therapy model. The use of high-frequency waves provided no additional bactericidal effect after root canal treatment. However, high-frequency wave irradiation was found to promote healing of periapical lesions on the host side through increased expression of fibroblast growth factor 2 and transforming growth factor-β1 and could therefore be useful as an adjuvant nonsurgical treatment for apical periodontitis. PLoS One 15 9 e0239660 null 20200000 10.1371/journal.pone.0239660 32991601 read0046145/published_papers/31371957 Rie Kido Yuka Hiroshima Jun-ichi Kido Takahisa Ikuta Eijiro Sakamoto Yuji Inagaki Koji Naruishi Hiromichi Yumoto Advanced glycation end-products increase lipocalin 2 expression in human oral epithelial cells Wiley Journal of Periodontal Research 1600-0765 55 4 539 550 20200000 10.1111/jre.12741 read0046145/published_papers/28425720 Ryosuke Takagi Eijiro Sakamoto Jun-Ichi Kido Yuji Inagaki Yuka Hiroshima Koji Naruishi Hiromichi Yumoto S100A9 Increases IL-6 and RANKL Expressions through MAPKs and STAT3 Signaling Pathways in Osteocyte-Like Cells. Objective: Calprotectin is a heterocomplex of S100A8 and S100A9 and is mainly secreted from neutrophils, monocytes, and chondrocytes in inflammatory condition. Calprotectin binds to RAGE and TLR4 and induces the expression of proinflammatory chemokines and cytokines in various cells. Periodontitis is a chronic inflammatory disease that leads to gingival inflammation and alveolar bone resorption. Calprotectin levels in gingival crevicular fluid of periodontitis patients are higher than healthy patients. In the present study, the effects of S100A8 and S100A9 on the expressions of proinflammatory cytokines and bone metabolism-related factors in mouse osteocyte-like cells (MLO-Y4-A2) were investigated. Design: MLO-Y4-A2 cells were treated with S100A8 and S100A9, and the expressions of RAGE, TLR4, RANKL, and several inflammatory cytokines were analyzed by PCR and Western blotting or ELISA methods. To investigate the intracellular signaling pathways, phosphorylation of MAPK and STAT3 was determined by Western blotting, and chemical specific inhibitors and siRNAs were used. Results: Expressions of IL-6 and RANKL were increased by treatment with S100A9 but not S100A8. However, both S100A8 and S100A9 did not change expression of IL-1β, IL-8, and TNF-α. Although RAGE and TLR4 expressions were not upregulated by S100A9 treatment, transfection of siRNA for RAGE and TLR4 significantly decreased IL-6 and RANKL expressions. In addition, S100A9 activated p38, ERK, and STAT3 signaling pathways, and inhibitors for these factors significantly decreased S100A9-induced IL-6 and RANKL expressions. Conclusions: These results indicated that S100A9 induces IL-6 and RANKL production via engagement with RAGE and TLR4 signalings in osteocytes and suggested that S100A9 may play important roles in the periodontal alveolar bone destruction. BioMed research international 2020 7149408 7149408 20200000 10.1155/2020/7149408 32149126 read0046145/published_papers/26667659 Yumi Kuwamura Sumiko Yoshida Kiyoe Kurahashi Masuko Sumikawa Eijiro Sakamoto Ken-ichi Aihara Hiromichi Yumoto Akio Kuroda Itsuro Endo Toshiyuki Yasui Sachi Kishida Modified diabetes oral health assessment tool (M-DiOHAT©) for nurses and their association with efficacy beliefs and outcome expectancies in patients with diabetes The Journal of Nursing Investigation 17 2 null null 20200100 read0046145/published_papers/28425724 Kohei Nonaka Mika Bando Eijiro Sakamoto Yuji Inagaki Koji Naruishi Hiromichi Yumoto Jun-Ichi Kido 6-Shogaol Inhibits Advanced Glycation End-Products-Induced IL-6 and ICAM-1 Expression by Regulating Oxidative Responses in Human Gingival Fibroblasts. Advanced glycation end-products (AGEs) cause diabetes mellitus (DM) complications and accumulate more highly in periodontal tissues of patients with periodontitis and DM. AGEs aggravate periodontitis with DM by increasing the expression of inflammation-related factors in periodontal tissues. 6-Shogaol, a major compound in ginger, has anti-inflammatory and anti-oxidative activities. However, the influence of shogaol on DM-associated periodontitis is not well known. In this study, the effects of 6-shogaol on AGEs-induced oxidative and anti-oxidative responses, and IL-6 and ICAM-1 expression in human gingival fibroblasts (HGFs) were investigated. When HGFs were cultured with 6-shogaol and AGEs, the activities of reactive oxygen species (ROS) and antioxidant enzymes (heme oxygenase-1 [HO-1] and NAD(P)H quinone dehydrogenase 1 [NQO1]), and IL-6 and ICAM-1 expressions were investigated. RAGE expression and phosphorylation of MAPKs and NF-κB were examined by western blotting. 6-Shogaol significantly inhibited AGEs-induced ROS activity, and increased HO-1 and NQO1 levels compared with the AGEs-treated cells. The AGEs-stimulated expression levels of receptor of AGE (RAGE), IL-6 and ICAM-1 and the phosphorylation of p38, ERK and p65 were attenuated by 6-shogaol. These results suggested that 6-shogaol inhibits AGEs-induced inflammatory responses by regulating oxidative and anti-oxidative activities and may have protective effects on periodontitis with DM. Molecules (Basel, Switzerland) 24 20 null null 20191015 10.3390/molecules24203705 31619000 read0046145/published_papers/39576878 二宮 雅美 生田 貴久 成石 浩司 湯本 浩通 重度慢性歯周炎を有する血液透析患者に対して包括的歯周治療を行った一症例 (NPO)日本歯科保存学会 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 151 61 61 20191000 read0046145/published_papers/26138223 Hiromichi Yumoto Takashi Uebansou Takaaki Shimohata Akira Takahashi Current understanding of the gut microflora in subjects with nutrition-associated metabolic disorder such as obesity and/or diabetes: Is there any relevance with oral microflora? Current Oral Health Reports 2196-3002 6 2 100 109 20190600 10.1007/s40496-019-0221-7 read0046145/published_papers/39576880 稲垣 裕司 生田 貴久 坂本 英次郎 木戸 淳一 湯本 浩通 全顎的歯周治療に伴って関節リウマチの状態が改善した一症例 (NPO)日本歯科保存学会 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 150 60 60 20190500 read0046145/published_papers/34329052 金崎 伸幸 二宮 雅美 湯本 浩通 矯正治療中に進行した侵襲性歯周炎患者に対し歯周組織再生療法を行った一症例 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 61 149 149 20190500 read0046145/published_papers/26440931 Sakamoto E Kido JI Takagi R Inagaki Y Naruishi K Nagata T Yumoto H Advanced glycation end-product 2 and Porphyromonas gingivalis lipopolysaccharide increase sclerostin expression in mouse osteocyte-like cells. Bone 8756-3282 122 22 30 20190500 10.1016/j.bone.2019.02.001 30735798 read0046145/published_papers/25265638 Natsumi Fujiwara Hiromichi Yumoto Koji Miyamoto Katsuhiko Hirota Hiromi Nakae Saya Tanaka Keiji Murakami Yasusei Kudo Kazumi Ozaki Yoichiro Miyake 2-Methacryloyloxyethyl phosphorylcholine (MPC)-polymer suppresses an increase of oral bacteria: a single-blind, crossover clinical trial. OBJECTIVES: The biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymers, which mimic a biomembrane, reduce protein adsorption and bacterial adhesion and inhibit cell attachment. The aim of this study is to clarify whether MPC-polymer can suppress the bacterial adherence in oral cavity by a crossover design. We also investigated the number of Fusobacterium nucleatum, which is the key bacterium forming dental plaque, in clinical samples. MATERIALS AND METHODS: This study was a randomized, placebo-controlled, single-blind, crossover study, with two treatment periods separated by a 2-week washout period. We conducted clinical trial with 20 healthy subjects to evaluate the effect of 5% MPC-polymer mouthwash after 5 h on oral microflora. PBS was used as a control. The bacterial number in the gargling sample before and after intervention was counted by an electronic bacterial counter and a culture method. DNA amounts of total bacteria and F. nucleatum were examined by q-PCR. RESULTS: The numbers of total bacteria and oral streptcocci after 5 h of 5% MPC-polymer treatment significantly decreased, compared to the control group. Moreover, the DNA amounts of total bacteria and F. nucleatum significantly decreased by 5% MPC-polymer mouthwash. CONCLUSIONS: We suggest that MPC-polymer coating in the oral cavity may suppress the oral bacterial adherence. CLINICAL RELEVANCE: MPC-polymer can be a potent compound for the control of oral microflora to prevent oral infection. Clinical oral investigations 1432-6981 23 2 739 746 20190200 10.1007/s00784-018-2490-2 29766378 read0046145/published_papers/31371955 Hitomi Kuramoto Kouji Hirao Hiromichi Yumoto Yuki Hosokawa Tadashi Nakanishi Daisuke Takegawa Ayako Washio Chiaki Kitamura Takashi Matsuo Caffeic acid phenethyl ester (CAPE) induces VEGF expression and production in rat odontoblastic cells BioMed Research International 5390720 null 20190000 read0046145/published_papers/31371952 Takuya Nii Hiromichi Yumoto Katsuhiko Hirota Yoichiro Miyake Anti-inflammatory effects of olanexidine gluconate on oral epithelial cells BMC Oral Health 19 239 null 20190000 read0046145/published_papers/31371950 Hiromichi Yumoto Katsuhiko Hirota Kouji Hirao Masami Ninomiya Keiji Murakami Hideki Fujii Yoichiro Miyake The Pathogenic Factors from Oral Streptococci for Systemic Diseases International Journal of Molecular Sciences 20 18 e4571 null 20190000 read0046145/published_papers/31371938 Keiko Aota Koichi Kani Tomoko Yamanoi Yukihiro Momota Masami Ninomiya Hiromichi Yumoto Masayuki Azuma Management of tooth extraction in a patient with ELANE gene mutation-induced cyclic neutropenia: a case report INTRODUCTION: Cyclic neutropenia (CyN) is a rare hematological disease, and patients with CyN often experience an early onset of severe periodontitis and are forced to undergo tooth extraction. Here, we report a case of a patient with CyN who showed different periodicity and oscillations of neutrophil count compared with her mother, despite sharing the same novel genetic mutation. PATIENT CONCERNS: A 17-year-old Japanese girl who had been diagnosed with CyN shortly after birth presented to our hospital with a complaint of mobility of her teeth and gingivitis. Upon presentation, an intraoral examination was performed and revealed redness and swelling of the marginal and attached gingiva. Radiographs revealed extreme resorption of the alveolar bone and apical lesions in her mandibular lateral incisors. The patient's hematologic data demonstrated a lack of blood neutrophils (0/μL). The patient had no history of dental extraction, and her mother also had a history of CyN. DIAGNOSES: The patient was diagnosed with severe periodontitis that was associated with CyN. Gene testing showed a novel heterozygous mutation in exon 4 of the ELANE gene (c.538delC, p.Leu180Ser fsX11). INTERVENTIONS: Based on the clinical findings, we planned to extract the patient's mandibular lateral incisors. Although the tooth extraction was scheduled considering the cyclic variation in neutrophil count, the patient's neutrophil count was 0/μL on the day before the planned extraction. Therefore, granulocyte-colony stimulating factor (G-CSF) was administered to increase the patient's neutrophil count. On the day of the patient's admission for the tooth extraction, she presented with fever (body temperature, 38.5°C), tonsillitis, and stomatitis. The extraction was subsequently delayed, and the patient was administered antibiotics and G-CSF for 4 days. At this time, the neutrophil count increased to 750/μL, and the tooth extraction was carried out safely. OUTCOMES: The postoperative course was uneventful, and the healing process at the extraction site was excellent. CONCLUSION: There is a possibility that the periodicity and oscillations of neutrophil count may change with growth in patients with CyN. Therefore, it is important to frequently examine and treat patients with fluctuating neutrophil levels for the management of invasive dental treatment in patients with CyN. Medicine 98 39 e17372 null 20190000 10.1097/MD.0000000000017372 31574885 read0046145/published_papers/39576889 二宮 雅美 生田 貴久 成石 浩司 湯本 浩通 口腔内細菌が原因で発症した感染性心内膜炎患者に対して包括的歯周治療を行った一症例 (NPO)日本歯科保存学会 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 149 163 163 20181000 read0046145/published_papers/39576896 木戸 理恵 坂本 英次郎 板東 美香 稲垣 裕司 二宮 雅美 美原 智恵 生田 貴久 成石 浩司 永田 俊彦 木戸 淳一 湯本 浩通 インプラント周囲溝滲出液中のカルプロテクチンとNTxを用いたインプラント疾患の診断 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 60 132 132 20180500 read0046145/published_papers/31372035 湯本浩通 口腔Biofilm感染症における病原因子と全身に及ぼす影響 Journal of Oral Health and Biosciences 31 1 1 12 20180000 read0046145/published_papers/31371933 Yoshihito Naito Hiromichi Yumoto Kiran Kumar H. S Takashi Matsuo Katsuhiko Hirota Yoichiro Miyake Kan Nagao Yoritoki Tomotake Ryo Jimbo Tetsuo Ichikawa Antifungal and mechanical properties of tissue conditioner containing plant-derived component: an in-vitro study Journal of Prosthodontics 27 665 669 20180000 read0046145/published_papers/31371928 Koji Naruishi Jun-ichi Kido Hiromichi Yumoto Clinical effects of low body mass index on geriatric status in elderly patients Experimental Gerontology 110 86 91 20180000 read0046145/published_papers/31371920 Shinya Horiuchi Hiromichi Yumoto Tomoko Kimura Minami Sato Silvia Naomi Mitsui Masahiro Hiasa Eiji Nishikawa Tarek El-Bialy Eiji Tanaka Effect of pulsed ultrasound toothbrush on Streptococcus mutans biofilm removal PURPOSE: To evaluate the effect of pulsed ultrasound toothbrush on the removal of biofilm formed by Streptococcus mutans (S. mutans). METHODS: S. mutans biofilm grown on apatite pellet was destructed with four different sonic action toothbrushes: 1) pulsed ultrasound with sonic vibration (PUV); 2) continuous ultrasound with sonic vibration (CUV); 3) sonic vibration only (SV); and 4) no ultrasound nor sonic vibration (control). After 3 minutes of noncontact brushing, the amount of water-insoluble glucan was measured, and the residual biofilm was observed by scanning electron microscopy. RESULTS: PUV group revealed the smallest amount of the residual water-insoluble glucans (32 ± 19%), followed by the CUV group (54 ± 12%) and the SV group (64 ± 13%). The PUV group showed a significantly lower amount of the residual water-insoluble glucan than the SV group, while no significant difference was found between SV and CUV. The bacterial adherence and aggregation notably decreased in the PUV group, compared to the remaining three groups. CLINICAL SIGNIFICANCE: The sonic vibration with pulsed ultrasound showed more reduction of the biofilm compared to the control and the sonic vibration with and without continuous ultrasound. Thus, pulsed ultrasound action may be beneficial for biofilm removal of interproximal regions. American Journal of Dentistry 0894-8275 31 2 67 70 20180000 29630788 read0046145/published_papers/27966735 藤原 奈津美 湯本 浩通 弘田 克彦 村上 圭史 尾崎 和美 三宅 洋一郎 宮本 幸治 中江 弘美 田中 沙弥 MPCポリマーによる口腔細菌の抑制効果 (一社)歯科基礎医学会 Journal of Oral Biosciences Supplement 2187-9109 2017 341 341 20170900 read0046145/published_papers/22768540 N. Fujiwara K. Murakami M. Nakao M. Toguchi H. Yumoto T. Amoh K. Hirota T. Matsuo S. Sano K. Ozaki Y. Miyake Novel reuterin-related compounds suppress odour by periodontopathic bacteria ORAL DISEASES 1601-0825 23 4 492 497 20170500 10.1111/odi.12638 read0046145/published_papers/22768538 K. Murakami H. Yumoto A. Murakami T. Amoh D. Viducic K. Hirota A. Tabata H. Nagamune H. Kourai T. Matsuo Y. Miyake Evaluation of the effectiveness of the potent bis-quaternary ammonium compound, 4,4-(,-hexametylenedithio) bis (1-octylpyridinium bromide) (4DTBP-6,8) on Pseudomonas aeruginosa JOURNAL OF APPLIED MICROBIOLOGY 1365-2672 122 4 893 899 20170400 10.1111/jam.13392 read0046145/published_papers/27982789 Yasuko Sekita Keiji Murakami Hiromichi Yumoto Kouji Hirao Takashi Amoh Natsumi Fujiwara Katsuhiko Hirota Hideki Fujii Takashi Matsuo Yoichiro Miyake Yoshiki Kashiwada Antibiofilm and Anti-Inflammatory Activities of Houttuynia cordata Decoction for Oral Care EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 1741-4288 null null 20170000 10.1155/2017/2850947 read0046145/published_papers/27606063 Heni Susilowati Keiji Murakami Hiromichi Yumoto Takashi Amoh Kouji Hirao Katsuhiko Hirota Takashi Matsuo Yoichiro Miyake Royal Jelly Inhibits Pseudomonas aeruginosa Adherence and Reduces Excessive Inflammatory Responses in Human Epithelial Cells BIOMED RESEARCH INTERNATIONAL 2314-6141 2017:3191752. null null 20170000 10.1155/2017/3191752 read0046145/published_papers/26084574 Kouji HIRAO 平尾 功治 Hiromichi YUMOTO 湯本 浩通 Yuki HOSOKAWA 細川 由樹 Hitomi KURAMOTO 蔵本 瞳 Ayako WASHIO 鷲尾 絢子 Tadashi NAKANISHI 中西 正 Daisuke TAKEGAWA 武川 大輔 Chiaki KITAMURA 北村 知昭 Takashi MATSUO 松尾 敬志 Anti-inflammatory Effects of Catechin on Rat Odontoblastic Cells (KN-3) ラット象牙芽細胞様細胞 (KN-3)におけるカテキンの抗炎症作用． <p> Purpose: Odontoblasts, which are located in the outermost layer of dental pulp, first recognize caries-related pathogens, sense such external mechanical and chemical irritations, and play important roles in the innate immune system of dental pulp tissues. We recently reported that nucleotide-binding oligomerization domain (NOD) 1, one of the pattern recognition receptors in innate immunity, is expressed in rat odontoblastic cells (KN-3) and functions to up-regulate the chemokines expression, and suggested that NOD1 may play important roles in the initiation and progression of pulpitis. It has been reported that epigallocatechin gallate (EGCG), one of the major active components of green tea catechin, has anti-inflammatory effects. The aim of this study was to determine the anti-inflammatory effects of EGCG on odontoblasts stimulated with NOD1-specific ligand or pro-inflammatory cytokines.</p><p> Methods: The cytotoxicity of EGCG to KN-3, rat odontoblastic cell line, was analyzed by lactate dehydrogenase (LDH) cytotoxicity assay. γ-D-diaminopimelic acid (iE-DAP), NOD1-specific ligand, tumor necrosis factor (TNF) -α- or interleukin (IL) -1β-stimulated KN-3 was treated with EGCG and then the expression and production of pro-inflammatory mediators, such as chemokines and inducible nitric oxide synthase (iNOS), were determined by real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), respectively.</p><p> Results: EGCG (up to 10μg/m<i>l</i>) had no cytotoxic effect on KN-3. EGCG significantly inhibited the production of chemokines, such as cytokine-induced neutrophil chemoattractant (CINC) -2 and C-C motif chemokine ligand (CCL) 20, in a dose-dependent manner. The expression of iNOS mRNA was also significantly reduced by treatment with EGCG at 10μg/m<i>l</i>.</p><p> Conclusions: These findings demonstrated that EGCG treatment significantly reduced the expression and production of various pro-inflammatory mediators via NOD1-mediated innate immune response as well as inflammatory response elicited by TNF-α or IL-1β stimulation in rat odontoblastic cells (KN-3), suggesting that EGCG might be useful therapeutically as an anti-inflammatory modulator of dental pulpal inflammation.</p> <p> 目的 : 象牙芽細胞は歯髄の最外層に存在し, 齲蝕関連病原因子や機械的刺激・化学的刺激に対して, 初期に反応し, 歯髄組織の自然免疫に重要な役割を担っている. われわれは, ラット象牙芽細胞様細胞 (KN-3) に自然免疫レセプターであるnucleotide-binding oligomerization domein (NOD) 1が発現, 機能していることを報告しており, NOD1は歯髄炎の発症, 進展に重要な役割を演じている可能性がある. 緑茶カテキンの一種であるepigallocatechin-3-gallate (EGCG) は抗炎症作用を有することが知られており, NOD1リガンドならびに炎症性サイトカインの刺激を受けた象牙芽細胞に対するEGCGの抗炎症作用について解析することを目的とし研究を行った. </p><p> 材料と方法 : EGCGのKN-3に対する細胞障害性を, lactate dehydrogenase (LDH) cytotoxicity assayを用いて調べた. NOD1特異的リガンドであるγ-D-diaminopimelic acid (iE-DAP) やtumor necrosis factor (TNF) -α, interleukin (IL) -1βにてKN-3を刺激し, 同時にEGCGを作用させた後, real-time polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA) を用い, ケモカインや炎症性メディエーターの発現や産生について解析を行った. </p><p> 成績 : 10μg/m<i>l</i>までのEGCGはKN-3への細胞障害性を認めなかった. iE-DAPや炎症性サイトカインで刺激したKN-3に対して, EGCGはcytokine-induced neutrophil chemoattractant (CINC) -2やC-C motif chemokine ligand (CCL) 20といったケモカインの産生を有意に抑制し, その作用は濃度依存的であった. さらに, EGCGはinducible nitric oxide synthase (iNOS) のmRNA発現も抑制した. </p><p> 結論 : 今回の研究において, EGCGがNOD1リガンドならびにサイトカイン刺激におけるラット象牙芽細胞様細胞 (KN-3) の種々の炎症性反応を抑制することが明らかとなった. この結果は, EGCGの応用が, 新たな歯髄炎の抗炎症療法となりうる可能性を示唆するものである. </p> The Japanese Society of Conservative Dentistry 特定非営利活動法人 日本歯科保存学会 The Japanese Journal of Conservative Dentistry 日本歯科保存学会雑誌 60 5 235 244 20170000 10.11471/shikahozon.60.235 read0046145/published_papers/22768539 Natsumi Fujiwara Antibacterial and antibiofilm effects of Reuterin-Related Compounds to periodontopathic bacteria Journal of Oral Health and Biosciences 30 1 8 17 20170000 10.20738/johb.30.1_8 read0046145/published_papers/28416205 藤原 奈津美 村上 圭史 湯本 浩通 弘田 克彦 尾崎 和美 三宅 洋一郎 中尾 允泰 佐野 茂樹 歯周病原菌に対する新規化合物の効果 (一社)歯科基礎医学会 Journal of Oral Biosciences Supplement 2187-9109 2016 370 370 20160900 read0046145/published_papers/46810716 弘田 克彦 湯本 浩通 村上 圭史 平尾 功治 天羽 崇 三宅 洋一郎 Streptococcus intermedius biofilm componentsの糖鎖結合能と炎症反応誘導能に関する解析 日本バイオフィルム学会 BACTERIAL ADHERENCE & BIOFILM 1348-6071 29 45 46 20160500 read0046145/published_papers/46810714 弘田 克彦 湯本 浩通 村上 圭史 平尾 功治 天羽 崇 三宅 洋一郎 常在菌と病原菌の狭間 口腔常在菌Streptococcus intermediusが呼吸器疾患重症化に関与するメカニズム 日本細菌学会 日本細菌学雑誌 1882-4110 71 1 45 45 20160200 read0046145/published_papers/22768536 Yuki Hosokawa Kouji Hirao Hiromichi Yumoto Ayako Washio Tadashi Nakanishi Daisuke Takegawa Chiaki Kitamura Takashi Matsuo Functional Roles of NOD1 in Odontoblasts on Dental Pulp Innate Immunity BIOMED RESEARCH INTERNATIONAL 2314-6141 null null 20160000 10.1155/2016/9325436 read0046145/published_papers/22768535 Yasuko Sekita Keiji Murakami Hiromichi Yumoto Takashi Amoh Natsumi Fujiwara Shohei Ogata Takashi Matsuo Yoichiro Miyake Yoshiki Kashiwada Preventive Effects of Houttuynia cordata Extract for Oral Infectious Diseases BIOMED RESEARCH INTERNATIONAL 2314-6141 null null 20160000 10.1155/2016/2581876 read0046145/published_papers/22768534 Yasuko Sekita Keiji Murakami Hiromichi Yumoto Hiroyuki Mizuguchi Takashi Amoh Satoshi Ogino Takashi Matsuo Yoichiro Miyake Hiroyuki Fukui Yoshiki Kashiwada Anti-bacterial and anti-inflammatory effects of ethanol extract from Houttuynia cordata poultice BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 1347-6947 80 6 1205 1213 20160000 10.1080/09168451.2016.1151339 read0046145/published_papers/46810718 弘田 克彦 湯本 浩通 村上 圭史 平尾 功治 天羽 崇 三宅 洋一郎 Streptococcus intermedius biofilm componentsの糖鎖結合能と炎症反応誘導能に関する解析 日本バイオフィルム学会 日本バイオフィルム学会学術集会プログラム・抄録集 29 34 34 20150700 read0046145/published_papers/46810719 弘田 克彦 湯本 浩通 村上 圭史 平尾 功治 天羽 崇 三宅 洋一郎 Streptococcus intermedius Histone-Like Proteinと細胞外ベシクルの特性 日本細菌学会 日本細菌学雑誌 1882-4110 70 1 167 167 20150200 read0046145/published_papers/18303733 Hiromichi Yumoto Katsuhiko Hirota Kouji Hirao Tsuyoshi Miyazaki Nobuyuki Yamamoto Koji Miyamoto Keiji Murakami Natsumi Fujiwara Takashi Matsuo Yoichiro Miyake Anti-inflammatory and protective effects of 2-methacryloyloxyethyl phosphorylcholine polymer on oral epithelial cells JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 1552-4965 103 2 555 563 20150200 10.1002/jbm.a.35201 read0046145/published_papers/18303734 Hiromichi Yumoto Kouji Hirao Toshihiko Tominaga Naoki Bando Kanako Takahashi Takashi Matsuo Electromagnetic Wave Irradiation Promotes Osteoblastic Cell Proliferation and Up-Regulates Growth Factors via Activation of the ERK1/2 and p38 MAPK Pathways CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 1421-9778 35 2 601 615 20150000 10.1159/000369722 read0046145/published_papers/18303732 Daisuke Takegawa 武川 大輔 Tadashi Nakanishi Kouji Hirao Hiromichi Yumoto Kanako Takahashi Takashi Matsuo Modulatory Roles of Interferon-gamma through Indoleamine 2, 3-dioxygenase Induction in Innate Immune Response of Dental Pulp Cells Modulatory Roles of Interferon-γ through Indoleamine 2, 3-dioxygenase Induction in Innate Immune Response of Dental Pulp Cells JOURNAL OF ENDODONTICS Journal of Endodontics 1878-3554 40 9 1382 1387 20140900 10.1016/j.joen.2014.03.018 read0046145/published_papers/18303730 B. Sapaar A. Nur K. Hirota H. Yumoto K. Murakami T. Amoh T. Matsuo T. Ichikawa Y. Miyake Effects of extracellular DNA from Candida albicans and pneumonia-related pathogens on Candida biofilm formation and hyphal transformation JOURNAL OF APPLIED MICROBIOLOGY 1365-2672 116 6 1531 1542 20140600 10.1111/jam.12483 read0046145/published_papers/18303731 Yiwei Wang The comparison of the ability of polysaccharide from different Porphyromonas gingivalis in inducing THP-1 cells to produce cytokines Chinese Journal of Stomatology 49 2 78 83 20140000 read0046145/published_papers/18303729 Dali Liu Yanbin Zhou Mariko Naito Hiromichi Yumoto Qiang Li Yoichiro Miyake Jingping Liang Rong Shu Draft genome sequence of Porphyromonas gingivalis strain SJD2, isolated from the periodontal pocket of a patient with periodontitis in China American Society for Microbiology Genome Announcements 2169-8287 2 1 e01091-13 null 20140000 10.1128/genomeA.e01091-13 2-s2.0-84998610618 read0046145/published_papers/18303728 A. Nur K. Hirota H. Yumoto K. Hirao D. Liu K. Takahashi K. Murakami T. Matsuo R. Shu Y. Miyake Effects of extracellular DNA and DNA-binding protein on the development of a Streptococcus intermedius biofilm Journal of Applied Microbiology 1364-5072 115 1 260 270 20130700 10.1111/jam.12202 23551549 2-s2.0-84879085342 read0046145/published_papers/27157790 松尾敬志 尾崎和美 中西 正 湯本浩通 高橋加奈子 平尾功治 武川大輔 歯髄疾患の病態と診断 歯髄疾患の原因は多様であるが，殆どの場合は齲蝕細菌に起因すると考えられる．歯の硬組織に付着した口腔内細菌が増殖し，硬組織表層を破壊して象牙質に侵入する．象牙質の細菌が更に進行すると，歯髄に炎症を惹起し，この進行が止まらなければ，やがて歯髄壊死に陥る．壊死した根管系に細菌が感染し，根管内よりこれらの細菌および病原性物質が根尖歯周組織に漏出して根尖性歯周炎が惹起されると考えられている．感染症という観点から，歯髄炎の病理・病態について検討し，診断・治療の問題点を考察した． 日本歯内療法学会 日本歯内療法学会雑誌 1347-8672 34 2 67 76 20130000 10.20817/jeajournal.34.2_67 read0046145/published_papers/47913595 Hiromichi Yumoto Kouji Hirao Takashi Matsuo Yoichiro Miyake The roles of cytokines in periodontal disease Nova Science Publishers, Inc. Cytokines: Mechanisms, Functions and Abnormalities 235 254 20120100 2-s2.0-84892088729 read0046145/published_papers/29183952 尾崎 和美 藤原 奈津美 石川 真琴 細川 育子 細川 義隆 湯本 浩通 う蝕関連細菌の家族内伝播と生活習慣による影響に関する検索 兄弟姉妹間の差異について (NPO)日本歯科保存学会 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 134 67 67 20110500 read0046145/published_papers/27921062 Kouji Hirao Hiromichi Yumoto Tadashi Nakanishi Kayo Mukai Kanako Takahashi Daisuke Takegawa Takashi Matsuo Tea catechins reduce inflammatory reactions via mitogen-activated protein kinase pathways in toll-like receptor 2 ligand-stimulated dental pulp cells. These findings suggest that catechins might be useful therapeutically as an anti-inflammatory modulator of dental pulpal inflammation. Life Sciences 1879-0631 86 17-18 654 660 20100424 10.1016/j.lfs.2010.02.017 20176036 read0046145/published_papers/27921064 Tadashi Nakanishi Kayo Mukai Hiromichi Yumoto Kouji Hirao Yoshitaka Hosokawa Takashi Matsuo Anti-inflammatory effect of catechin on cultured dental pulp cells affected by bacteria-derived factors Catechins (bioactive polyphenols in green tea) are known to exhibit potent anti-inflammatory properties. However, the anti-inflammatory effects of catechins on inflamed dental pulp tissue are not known. In this study, we investigated the effect of epigallocatechin-3-gallate (EGCG) and epicatechin gallate (ECG), the major components of green tea catechins, on the expression of pro-inflammatory cytokines and adhesion molecules in human dental pulp cells stimulated with bacteria-derived factors such as lipopolysaccharide (LPS) and peptidoglycan (PG). The expression of interleukin (IL)-6 and of IL-8 was examined using the reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assays. The expression of intercellular adhesion molecule-1 (ICAM-1) and of vascular cell adhesion molecule-1 (VCAM-1) on dental pulp cells was analyzed using flow cytometry. The presence of EGCG and ECG significantly reduced, in a concentration-dependent manner, the expression of IL-6 and IL-8 in dental pulp cells exposed to LPS or PG. Increased expression of ICAM-1 and VCAM-1 on the dental pulp cells in response to bacterial components was also decreased by treatment with EGCG and ECG. These findings suggest that green tea catechins may prevent the exacerbation of pulpitis. European Journal of Oral Sciences 1600-0722 118 2 145 150 20100400 10.1111/j.1600-0722.2010.00714.x 20487003 read0046145/published_papers/27921072 Kouji Hirao Hiromichi Yumoto Kanako Takahashi Kayo Mukai Tadashi Nakanishi Takashi Matsuo Roles of TLR2, TLR4, NOD2, and NOD1 in pulp fibroblasts. Pulp fibroblasts express various pro-inflammatory mediators leading to marked infiltration of inflammatory cells in the progression of pulpitis. We hypothesized that pulp fibroblasts play roles in the recognition of invaded caries-related bacteria and the subsequent innate immune responses. We found clear expressions of TLR2, NOD1, and NOD2 and a faint expression of TLR4 in human dental pulp fibroblasts (HDPF) by RT-PCR and flow cytometry. We also observed that various pro-inflammatory mediators, including cytokines, chemokines, adhesion molecules, prostaglandin E(2) and its key enzyme COX-2, not iNOS or caspase-1, were markedly up-regulated by stimulation with these TLR and NOD agonists. More over, the NOD2 agonist acted synergistically with the TLR2, not the TLR4, agonist to stimulate the production of pro-inflammatory mediators in HDPF. These findings indicate that TLR2, TLR4, NOD2, and NOD1 in HDPF are functional receptors, and NOD2 is a modulator of signals transmitted through TLR2 in pulpal immune responses, leading to progressive pulpitis. Journal of Dental Research 1544-0591 88 8 762 767 20090800 10.1177/0022034509341779 19734466 read0046145/published_papers/27921074 Kanako Takahashi Tadashi Nakanishi Hiromichi Yumoto Tomohiko Adachi Takashi Matsuo CCL20 production is induced in human dental pulp upon stimulation by Streptococcus mutans and pro-inflammatory cytokines Pulpitis is characterized by the marked infiltration of inflammatory cells in response to an invasion of caries-related bacteria. It is well known that chemokines regulate the trafficking of lymphocytes, and CC chemokine ligand 20 (CCL20) has been recently shown to play a crucial role in the recruitment of memory T cells and immature dendritic cells into inflammatory lesions. We previously reported that CCL20 was mainly expressed in microvascular endothelial cells and macrophages that accumulated in inflamed pulp tissues and that its specific receptor, CCR6, was expressed on infiltrated lymphocytes. However, the mechanism of CCL20 expression remains unclear. In this study, we investigated the expression of CCL20 in monocytes/macrophages, endothelial cells, and pulpal fibroblasts after stimulation with Streptococcus mutans, a representative of caries-related bacteria, or proinflammatory cytokines. CCL20 messenger RNA was detected by reverse transcription-polymerase chain reaction in inflamed pulp, but not in clinically normal pulp. By enzyme-linked immunosorbent assay, S. mutans induced a human monocytic cell line, differentiated macrophage-like THP-1 cells, and human umbilical vein endothelial cells (HUVEC) to produce an increased amount of CCL20. Lipoteichoic acid from S. mutans also elicited CCL20 production by HUVEC. Moreover, CCL20 production from pulpal fibroblasts was increased by stimulation with inetrleukin-1beta and tumor necrosis factor-alpha. Our results indicate that CCL20 expression is induced by stimulation with caries-related bacteria that have invaded deeply into the dentinal tubules as well as by proinflammatory cytokines in the inflamed pulpal lesions. It may be involved in the progression of pulpitis via accumulation of inflammatory cells. Oral Microbiology and Immunology 1399-302X 23 4 320 327 20080800 10.1111/j.1399-302X.2008.00431.x 18582332 read0046145/published_papers/34452410 Yoneda Miho 米田 美穂 Nakae Hideaki 中江 英明 Yumoto Hiromichi 湯本 浩通 Shinohara Chihiro 篠原 千尋 Matsuo Takashi 松尾 敬志 Inflammatory cytokines effect the expression patterns of claudin family, E-cadherin, JAM-A and occludin in human gingival epithelial cells. 炎症性サイトカインがヒト歯肉上皮細胞間接着機構に及ぼす影響 JAPANESE SOCIETY OF PERIODONTOLOGY 特定非営利活動法人 日本歯周病学会 Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology 特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集 2007 0 89 89 20070000 10.14833/amjsp.2007s.0.89.0 read0046145/published_papers/29184926 平尾 功治 湯本 浩通 高橋 加奈子 足立 智彦 向井 佳代 松尾 敬志 培養歯髄細胞におけるPattern Recognition Receptor(PRR)の発現と機能解析 (NPO)日本歯科保存学会 日本歯科保存学雑誌 2188-0808 49 84 84 20060400 read0046145/published_papers/29184933 足立 智彦 平尾 功治 湯本 浩通 高橋 加奈子 向井 佳代 中西 正 中江 英明 松尾 敬志 歯髄組織および細菌刺激THP-1細胞におけるCXCR3 ligands(特にIP-10)の発現解析 (NPO)日本歯科保存学会 日本歯科保存学雑誌 2188-0808 48 218 218 20051000 read0046145/published_papers/34403963 山下 美由紀 渡邊 久恵 三木 千津 石川 清美 久保 万友美 蓮井 義則 湯本 浩通 松尾 敬志 歯科衛生士の卒後研修の重要性とその意義 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 44 204 204 20020300 read0046145/published_papers/34403965 久保 万友美 渡邊 久恵 三木 千津 石川 清美 石川 利栄子 蓮井 義則 湯本 浩通 松尾 敬志 在宅患者の口腔ケアにおける歯科衛生士の役割 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 43 211 211 20010900 read0046145/published_papers/34403967 三木 千津 渡邊 久恵 石川 清美 石川 利栄子 石丸 毅 蓮井 義則 湯本 浩通 松尾 敬志 歯科衛生士の海外卒後研修に参加して 歯周組織・口腔粘膜疾患と国際協力について (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 43 189 189 20010300 read0046145/published_papers/34403969 石川 清美 渡邊 久恵 三木 千津 久保 万友美 石丸 毅 石川 利栄子 蓮井 義則 湯本 浩通 松尾 敬志 歯周病患者に対する禁煙指導について ニコチネルの応用 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 42 217 217 20000900 read0046145/published_papers/34403972 久保 万友美 渡邊 久恵 三木 千津 石川 清美 石丸 毅 石川 利栄子 蓮井 義則 湯本 浩通 松尾 敬志 瘢痕性類天疱瘡を有する患者の口腔管理 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 42 186 186 20000400 read0046145/published_papers/34403981 三木 千津 石川 清美 渡邊 久恵 石丸 毅 石川 利栄子 蓮井 義則 湯本 浩通 松尾 敬志 臼歯部の動揺を伴う歯周病患者への対応 特にメインテナンスにおける衛生士の役割 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 41 209 209 19990900 read0046145/published_papers/34403983 渡邊 久恵 三木 千津 石川 清美 石丸 毅 石川 利栄子 蓮井 義則 湯本 浩通 松尾 敬志 唾液分泌低下を伴う乳癌術後患者の口腔管理 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 41 205 205 19990300 read0046145/misc/48545046 松田 真司 湯本 浩通 小松 康高 出分 菜々衣 岩田 隆紀 長野 孝俊 両角 俊哉 後藤 亮真 加藤 幸紀 山下 元三 林 丈一朗 関野 愉 山下 明子 山下 慶子 吉村 篤利 菅谷 勉 高柴 正悟 田口 洋一郎 根本 英二 沼部 幸博 河口 浩之 学会主導型多施設共同研究 全身性疾患への影響を考慮した新たな歯周病重症度検査項目の策定 口腔内検査データ解析 (NPO)日本歯周病学会 日本歯周病学会会誌 1880-408X 66 125 125 20240400 read0046145/misc/48475608 木戸理恵 廣島佑香 木戸淳一 吉田賀弥 生田貴久 湯本浩通 人工合成したSecretory leukocyte protease inhibitorの抗菌活性とリポソーム封入 日本歯周病学会会誌(Web) 1880-408X 65 null null 20230000 read0046145/misc/48475576 廣島佑香 木戸理恵 木戸淳一 稲垣裕司 成石浩司 湯本浩通 人工合成したLipocalin 2はPorphyromonas gingivalisの口腔上皮細胞への付着を抑制する 日本歯周病学会会誌(Web) 1880-408X 63 null null 20210000 read0046145/misc/35026250 西川泰史 二宮雅美 川野弘道 木戸理恵 友竹偉則 湯本浩通 限局型侵襲性歯周炎患者に対してリグロスとサイトランスグラニュールを併用した歯周組織再生療法とインプラント治療を行った1症例 日本歯周病学会会誌(Web) 1880-408X 63 null null 20210000 read0046145/misc/48475591 木戸理恵 廣島佑香 生田貴久 稲垣裕司 板東美香 成石浩司 木戸淳一 湯本浩通 最終糖化産物による口腔上皮細胞のLipocalin2発現誘導は好中球の遊走性とサイトカイン発現を調節する 日本歯周病学会会誌(Web) 1880-408X 62 null null 20200000 read0046145/misc/35026257 木戸理恵 木戸淳一 坂本英次郎 西川泰史 廣島佑香 湯本浩通 イムノクロマト法を用いたカルプロテクチンの測定によるインプラント疾患の診断 日本歯周病学会会誌(Web) 1880-408X 62 null null 20200000 read0046145/misc/31371997 坂本英次郎 廣島佑香 木戸淳一 西川泰史 成石浩司 木戸理恵 湯本浩通 カルプロテクチンの歯周病病態における多様な役割と歯周病診断マーカーとしての可能性 日本歯周病学会会誌 62 4 193 199 20200000 read0046145/misc/31371991 湯本浩通 平尾功治 富永敏彦 松尾敬志 高周波電流の根尖性歯周炎への応用 歯内療法学会雑誌 41 2 82 90 20200000 read0046145/misc/31371988 平尾功治 二宮雅美 湯本浩通 X線写真で認められた根尖部透過像 DENTAL DIAMIND 45 663 21 22 20200000 read0046145/misc/31371982 成石浩司 坂本英次郎 生田貴久 木戸理恵 木戸淳一 湯本浩通 講義および実習試験の評価による歯周病学教育のあり方の考察 (NPO)日本歯科保存学会 日本歯科保存学雑誌 2188-0808 63 1 22 29 20200000 read0046145/misc/25893156 Masami Ninomiya 二宮 雅美 Tsutomu Iwamoto 岩本 勉 Hiromichi Yumoto 湯本 浩通 女児に認められた上顎前歯部の歯肉腫脹 DENTAL DIAMOND デンタルダイヤモンド 0386-2305 44 4 15 16 20190301 read0046145/misc/48475582 木戸理恵 廣島佑香 生田貴久 吉田賀弥 稲垣裕司 成石浩司 尾崎和美 木戸淳一 湯本浩通 最終糖化産物はヒト口腔上皮細胞のリポカリン2発現を増加する 日本歯周病学会会誌(Web) 1880-408X 61 null null 20190000 read0046145/misc/31371975 成石浩司 湯本浩通 歯周病と慢性腎疾患 月刊糖尿病 11 4 80 85 20190000 read0046145/misc/27921079 Hiromichi Yumoto Kouji Hirao Yuki Hosokawa Hitomi Kuramoto Daisuke Takegawa Tadashi Nakanishi Takashi Matsuo The Roles of Odontoblasts in Dental Pulp Innate Immunity Japanese Dental Science Review 54 3 105 117 20180800 read0046145/misc/26437603 Masami Ninomiya 二宮 雅美 Hiromichi Yumoto 湯本 浩通 歯根側面に認められたX線透過像 DENTAL DIAMOND デンタルダイヤモンド 0386-2305 43 4 17 18 20180300 read0046145/misc/48475600 木戸理恵 坂本英次郎 板東美香 稲垣裕司 二宮雅美 美原智恵 生田貴久 成石浩司 永田俊彦 木戸淳一 湯本浩通 インプラント周囲溝滲出液中のカルプロテクチンとNTxを用いたインプラント疾患の診断 日本歯周病学会会誌(Web) 1880-408X 60 null null 20180000 read0046145/misc/35026272 稲垣裕司 野中康平 高木亮輔 西川泰史 木戸理恵 坂本英次郎 成石浩司 木戸淳一 湯本浩通 甘露飲エキス配合製剤はラット実験的歯周炎において歯槽骨吸収を抑制する 日本歯周病学会会誌(Web) 1880-408X 60 null null 20180000 read0046145/misc/35026267 稲垣裕司 高木亮輔 西川泰史 木戸理恵 坂本英次郎 成石浩司 木戸淳一 湯本浩通 甘露飲エキスは破骨細胞と骨芽細胞の分化制御を介してラット実験的歯周炎の歯槽骨吸収を抑制する 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web) 149th null null 20180000 read0046145/misc/35026261 西川泰史 成石浩司 木戸淳一 湯本浩通 歯肉線維芽細胞においてカルプロテクチン誘導性炎症関連因子の産生に及ぼす高グルコースの影響 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web) 148th null null 20180000 read0046145/misc/22768537 K. Hirota H. Yumoto B. Sapaar T. Matsuo T. Ichikawa Y. Miyake Pathogenic factors in Candida biofilm-related infectious diseases JOURNAL OF APPLIED MICROBIOLOGY 1365-2672 122 2 321 330 20170200 10.1111/jam.13330 read0046145/misc/39701388 松井 沙織 呉本 勝隆 米田 直道 恵比須 繁之 湯本 浩通 的場 一成 石本 卓也 中野 貴由 野杁 由一郎 林 美加子 新規開発したラット根管治療モデルを用いた電磁波根尖療法の評価 (NPO)日本歯科保存学会 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 145 38 38 20161000 read0046145/misc/22768532 Tsuyoshi Miyagawa Tsuyoshi Fujita Hiromichi Yumoto Tetsuya Yoshimoto Mikihito Kajiya Kazuhisa Ouhara Shinji Matsuda Hideki Shiba Takashi Matsuo Hidemi Kurihara Azithromycin recovers reductions in barrier function in human gingival epithelial cells stimulated with tumor necrosis factor-alpha ARCHIVES OF ORAL BIOLOGY 1879-1506 62 64 69 20160200 10.1016/j.archoralbio.2015.11.015 read0046145/misc/22768533 Takashi matsuo 松尾 敬志 Conception of Infected Root Canal Treatment It is a well-established fact that bacterial infection of the dental pulp ultimately results in the formation of apical periodontitis. And apical periodontitis may develop periapical leisons consisting granulomas and cysts. In this article, we show the pathologic condition of infected root canal systems and periapical lesions immunohistologically. We also describe the possible causes of refractory apical periodontitis that conventional root canal therapy could not work effectively. Finally,we explain our newly developing treatment for refractory apical periodontitis named electro-magnetic apical treatment, and illustrate clinical efficacy of this treatment. 四国歯学会 Journal of Oral Health and Biosciences 2188-7888 28 2 87 92 20160000 read0046145/misc/22768531 Sang Hyuk Park Ling Ye Robert M. Love Jean-Christophe Farges Hiromichi Yumoto Inflammation of the Dental Pulp MEDIATORS OF INFLAMMATION 1466-1861 null null 20150000 10.1155/2015/980196 read0046145/misc/31372050 Asikin Nur Katsuhiko Hirota Hiromichi Yumoto Kouji Hirao Kanako Takahashi Keiji Murakami Takashi Matsuo Yoichiro Miyake Effect of extracellular DNA on pyocyanin production from Pseudomonas aeruginosa BACTERIAL ADHERENCE & BIOFILM 27 45 47 20140000 read0046145/misc/13708056 Jing Li Katsuhiko Hirota Takaharu Goto Hiromichi Yumoto Yoichiro Miyake Tetsuo Ichikawa Biofilm formation of Candida albicans on implant overdenture materials and its removal JOURNAL OF DENTISTRY 0300-5712 40 8 686 692 20120800 10.1016/j.jdent.2012.04.026 read0046145/misc/13708057 H. Yumoto T. Tominaga K. Hirao T. Kimura K. Takahashi T. Sumitomo N. Bando T. Matsuo Bactericidal activity and oral pathogen inactivation by electromagnetic wave irradiation JOURNAL OF APPLIED MICROBIOLOGY 1364-5072 113 1 181 191 20120700 10.1111/j.1365-2672.2012.05307.x 22507081 read0046145/misc/13708054 T. Fujita H. Yumoto H. Shiba K. Ouhara T. Miyagawa T. Nagahara S. Matsuda H. Kawaguchi T. Matsuo S. Murakami H. Kurihara Irsogladine maleate regulates epithelial barrier function in tumor necrosis factor-alpha-stimulated human gingival epithelial cells JOURNAL OF PERIODONTAL RESEARCH 0022-3484 47 1 55 61 20120200 10.1111/j.1600-0765.2011.01404.x 21895660 read0046145/misc/31372063 Asikin Nur Katsuhiko Hirota Hiromichi Yumoto Kouji Hirao Kanako Takahashi Keiji Murakami Takashi Matsuo Yoichiro Miyake Role of extracellular DNA and DNA-binding protein in biofilm formation of Streptococcus intermedius. BACTERIAL ADHERENCE & BIOFILM 26 25 29 20120000 read0046145/misc/13708052 Tadashi Nakanishi Daisuke Takegawa Kouji Hirao Kanako Takahashi Hiromichi Yumoto Takashi Matsuo Roles of dental pulp fibroblasts in the recognition of bacterium-related factors and subsequent development of pulpitis Japanese Dental Science Review 1882-7616 47 2 161 166 20110800 10.1016/j.jdsr.2011.02.001 2-s2.0-79960746217 read0046145/misc/13708051 K. Hirota H. Yumoto K. Miyamoto N. Yamamoto K. Murakami Y. Hoshino T. Matsuo Y. Miyake MPC-Polymer Reduces Adherence and Biofilm Formation by Oral Bacteria JOURNAL OF DENTAL RESEARCH 0022-0345 90 7 900 905 20110700 10.1177/0022034511402996 read0046145/misc/13708053 Naoki BANDO 坂東 直樹 Toshihiko TOMINAGA 富永 敏彦 Hiromichi YUMOTO 湯本 浩通 Takashi SUMITOMO 住友 孝史 Saki HIRAO 平尾 早希 Kouji HIRAO 平尾 功治 Takashi MATSUO 松尾 敬志 Application of electro-magnetic wave irradiation for endodontic treatment 電磁波照射の歯内療法への応用ーEMAT (Electro-Magnetic Apical Treatment)ー <p><b>Abstract : </b>Periapical periodontitis is considered to be caused by infectious agents persisting in the root canal system triggering the host immune reaction. Disinfection is the key point for endodontic treatment, but it is difficult to completely remove all causal agents from the root canal system because of the morphological complexities. In this study, we focused on the potential usefulness of electro-magnetic wave irradiation (EMWI) for clinical application to promote healing. The aims of this study were : 1) to determine the characteristics of EMW and 2) to evaluate the clinical application of EMWI to endodontic treatment. The analysis of EMW characteristics demonstrated that it is important to make the diameter of the active electrode tip smaller and to keep the insertion depth within 5 mm for effective generation of Joule heat, and revealed that Joule heat conduction spreads 1.0-1.5 mm wide in the horizontal direction. The success rate of clinical application of EMWI was 99.1%, which is higher than that of the EMWI-untreated group (95.1%). Moreover, the reduction rate of the diameter of periapical lesion in the EMWI-treated group (75.62%) was significantly increased. These findings demonstrate that EMWI accelerates healing of periapical lesions and suggest that it may be useful for clinical application to refractory periapical periodontitis as non-surgical treatment.</p> Japan Endodontic Association 一般社団法人 日本歯内療法学会 The Journal of Japan Endodontic Association 日本歯内療法学会雑誌 32 3 184 200 20110000 10.20817/jeajournal.32.3_184 read0046145/misc/13708050 J. Li K. Hirota H. Yumoto T. Matsuo Y. Miyake T. Ichikawa Enhanced germicidal effects of pulsed UV-LED irradiation on biofilms JOURNAL OF APPLIED MICROBIOLOGY 1364-5072 109 6 2183 2190 20101200 10.1111/j.1365-2672.2010.04850.x 20854456 read0046145/misc/23174379 Katsuhiko Hirota Takeyoshi Yoneyama Mayumi Sakamoto Hiroshi Miyamoto Masanori Kurihara Shizuo Kayama Keiji Murakami Hiromichi Yumoto Takashi Matsu Yoichiro Miyake High Prevalence of Pseudomonas aeruginosa From Oropharyngeal Biofilm in Patients With Cerebrovascular Infarction and Dysphagia CHEST 0012-3692 138 1 237 238 20100700 10.1378/chest.10-0240 read0046145/misc/23174380 Kouji Hirao Hiromichi Yumoto Tadashi Nakanishi Kayo Mukai Kanako Takahashi Daisuke Takegawa Takashi Matsuo Tea catechins reduce inflammatory reactions via mitogen-activated protein kinase pathways in toll-like receptor 2 ligand-stimulated dental pulp cells LIFE SCIENCES 0024-3205 86 17-18 654 660 20100400 10.1016/j.lfs.2010.02.017 20176036 read0046145/misc/23174378 Tadashi Nakanishi Kayo Mukai Hiromichi Yumoto Kouji Hirao Yoshitaka Hosokawa Takashi Matsuo Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors EUROPEAN JOURNAL OF ORAL SCIENCES 0909-8836 118 2 145 150 20100400 10.1111/j.1600-0722.2010.00714.x read0046145/misc/23174377 I. Hosokawa Y. Hosokawa K. Ozaki H. Yumoto H. Nakae T. Matsuo Proinflammatory effects of muramyldipeptide on human gingival fibroblasts JOURNAL OF PERIODONTAL RESEARCH 0022-3484 45 2 193 199 20100400 10.1111/j.1600-0765.2009.01217.x read0046145/misc/31372076 弘田克彦 湯本浩通 星野由美 村上圭史 宮本幸治 松尾敬志 三宅洋一郎 水溶性MPCポリマーの口腔細菌付着抑制効果 BACTERIAL ADHERENCE & BIOFILM 24 47 49 20100000 read0046145/misc/23174376 K. Hirao H. Yumoto K. Takahashi K. Mukai T. Nakanishi T. Matsuo Roles of TLR2, TLR4, NOd2, and NOd1 in Pulp Fibroblasts JOURNAL OF DENTAL RESEARCH 0022-0345 88 8 762 767 20090800 10.1177/0022034509341779 read0046145/misc/23174375 Dali Liu Hiromichi Yumoto Keiji Murakami Katsuhiko Hirota Shizuo Kayama Tomonori Taniguchi Akitake Yamamoto Tsuneko Ono Takashi Matsuo Yoichiro Miyake Heterologous expression of a histone-like protein from Streptococcus intermedius in Escherichia coli alters the nucleoid structure and inhibits the growth of E-coli FEMS MICROBIOLOGY LETTERS 0378-1097 288 1 68 75 20081100 10.1111/j.1574-6968.2008.01327.x read0046145/misc/23174373 K. Takahashi T. Nakanishi H. Yumoto T. Adachi T. Matsuo CCL20 production is induced in human dental pulp upon stimulation by Streptococcus mutans and proinflammatory cytokines ORAL MICROBIOLOGY AND IMMUNOLOGY 0902-0055 23 4 320 327 20080800 10.1111/j.1399-302X.2008.00431.x read0046145/misc/23174374 Dali Liu Hiromichi Yumoto Keiji Murakami Katsuhiko Hirota Tsuneko Ono Hideaki Nagamune Shizuo Kayama Takashi Matsuo Yoichiro Miyake The essentiality and involvement of Streptococcus intermedius histone-like DNA-binding protein in bacterial viability and normal growth MOLECULAR MICROBIOLOGY 0950-382X 68 5 1268 1282 20080600 10.1111/j.1365-2958.2008.06232.x 18410499 read0046145/misc/38520183 MUKAI Kayo 向井 佳代 NAKANISHI Tadashi 中西 正 YUMOTO Hiromichi 湯本 浩通 TAKAHASHI Kanako 高橋 加奈子 HIRAO Kouji 平尾 功治 MATSUO Takashi 松尾 敬志 Effect of catechins on cytokine production in human dental pulp cells カテキンがヒト培養歯髄細胞のサイトカイン産生に与える影響 : 石灰化誘導培地を用いた環境下において 日本歯科保存学雑誌 0387-2343 51 137 137 20080507 read0046145/misc/23174372 Takashi Ukai Hiromichi Yumoto Frank C. Gibson Caroline Attardo Genco Macrophage-elicited osteoclastogenesis in response to bacterial stimulation requires toll-like receptor 2-dependent tumor necrosis factor-alpha production INFECTION AND IMMUNITY 0019-9567 76 2 812 819 20080200 10.1128/IAI.01241-07 17998311 read0046145/misc/23174371 Xinyan Liu Takashi Ukai Hiromichi Yurnoto Michael Davey Sulip Goswami Frank C. Gibson Caroline A. Genco Toll-like receptor 2 plays a critical role in the progression of atherosclerosis that is independent of dietary lipids ATHEROSCLEROSIS 0021-9150 196 1 146 154 20080100 10.1016/j.atherosclerosis.2007.03.025 read0046145/misc/23174370 Dali Liu Hiromichi Yumoto Katsuhiko Hirota Keiji Murakami Kanako Takahashi Kouji Hirao Takashi Matsuo Kazuto Ohkura Hideaki Nagamune Yoichiro Miyake Histone-like DNA binding protein of Streptococcus intermedius induces the expression of pro-inflammatory cytokines in human monocytes via activation of ERK1/2 and JNK pathways CELLULAR MICROBIOLOGY 1462-5814 10 1 262 276 20080100 10.1111/j.1462-5822.2007.01040.x read0046145/misc/23174369 T. Adachi T. Nakanishi H. Yumoto K. Hirao K. Takahashi K. Mukai H. Nakae T. Matsuo Caries-related bacteria and cytokines induce CXCL10 in dental pulp JOURNAL OF DENTAL RESEARCH 0022-0345 86 12 1217 1222 20071200 10.1177/154405910708601215 read0046145/misc/39382213 HIRAO Kouji 平尾 功治 YUMOTO Hiromichi 湯本 浩通 MUKAI Kayo 向井 佳代 TAKAHASHI Kanako 高橋 加奈子 NAKANISHI Tadashi 中西 正 MATSUO Takashi 松尾 敬志 Catechin modulates the responses of cultured pulp cell stimulated with bacteria or bacterial components カテキンは細菌やその構成分子に対する培養歯髄細胞の反応を調節する 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY 0387-2343 50 128 128 20071005 read0046145/misc/39382194 YUMOTO Hiromichi 湯本 浩通 HIRAO Kouji 平尾 功治 TAKAHASHI Kanako 高橋 加奈子 MUKAI Kayo 向井 佳代 NAKANISHI Tadashi 中西 正 MATSUO Takashi 松尾 敬志 Expression of Cellular Adhesion Molecules via Pattern Recognition Receptor in Cultured Pulp Cells 培養歯髄細胞における Pattern Recognition Receptor (PRR) を介した接着分子の発現 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY 0387-2343 50 110 110 20071005 read0046145/misc/39392146 劉 大力 村上 圭史 湯本 浩通 弘田 克彦 三宅 洋一郎 Investigation of the Essentiality of Histone-Like Protein in Streptocuccus intermedius 日本細菌学雑誌 0021-4930 62 1 138 138 20070225 read0046145/misc/23174368 H. Yumoto M. Yamada C. Shinohara H. Nakae K. Takahashi H. Azakami S. Ebisu T. Matsuo Soluble products from Eikenella corrodens induce cell proliferation and expression of interleukin-8 and adhesion molecules in endothelial cells via mitogen-activated protein kinase pathways ORAL MICROBIOLOGY AND IMMUNOLOGY 0902-0055 22 1 36 45 20070200 10.1111/j.1399-302X.2007.00320.x read0046145/misc/39293729 HIRAO Kouji 平尾 功治 YUMOTO Hiromichi 湯本 浩通 TAKAHASHI Kanako 高橋 加奈子 MUKAI Kayo 向井 佳代 MATSUO Takashi 松尾 敬志 The effect of dental caries related bacteria on innate immune response in human dental pulp cell う蝕関連細菌が歯髄細胞における自然免疫応答に及ぼす影響 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY 0387-2343 49 173 173 20061011 read0046145/misc/23174367 Piotr Mydel Yusuke Takahashi Hiromichi Yumoto Maryta Sztukowska Malgorzata Kubica Frank C. Gibson Donald M. Kurtz Jim Travis L. Vincent Collins Ky-Anh Nguyen Caroline Attardo Genco Jan Potempa Roles of the host oxidative immune response and bacterial antioxidant rubrerythrin during Porphyromonas gingivalis infection PLOS PATHOGENS 1553-7374 2 7 712 725 20060700 10.1371/journal.ppat.0020076 read0046145/misc/23174365 Y Takahashi M Davey H Yumoto FC Gibson CA Genco Fimbria-dependent activation of pro-inflammatory molecules in Porphyromonas gingivalis infected human aortic endothelial cells CELLULAR MICROBIOLOGY 1462-5814 8 5 738 757 20060500 10.1111/j.1462-5822.2005.00661.x read0046145/misc/34452395 YONEDA Miho 米田 美穂 NAKAE Hideaki 中江 英明 YUMOTO Hiromichi 湯本 浩通 SHINOHARA Chihiro 篠原 千尋 MATSUO Takashi 松尾 敬志 Expression of Tight junction and adherens junction proteins in human periodontally diseased sites 歯周病変局所における上皮細胞間接着機構の動態 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY 0387-2343 49 33 33 20060421 read0046145/misc/23174366 FC Gibson H Yumoto Y Takahashi HH Chou CA Genco Innate immune signaling and Porphyromonas gingivalis-accelerated atherosclerosis JOURNAL OF DENTAL RESEARCH 0022-0345 85 2 106 121 20060200 10.1177/154405910608500202 read0046145/misc/23174364 T Miyamoto H Yumoto Y Takahashi M Davey FC Gibson CA Genco Pathogen-accelerated atherosclerosis occurs early after exposure and can be prevented via immunization INFECTION AND IMMUNITY 0019-9567 74 2 1376 1380 20060200 10.1128/IAI.74.2.1376-1380.2006 16428788 read0046145/misc/23174350 心臓血管疾患と歯周病 –動脈硬化症への歯周病原性細菌感染のかかわり- the Quintessence 25 5 174 183 20060000 read0046145/misc/23174331 Cardiovascular Disease and Periodontal Disease, Role of Infection from Periodontal Pathogen for the Acceleration of Atherosclerosis the Quintessence 25 5 1110 1119 20060000 read0046145/misc/23174363 H Yumoto HH Chou Y Takahashi M Davey FC Gibson CA Genco Sensitization of human aortic endothelial cells to lipopolysaccharide via regulation of Toll-like receptor 4 by bacterial fimbria-dependent invasion INFECTION AND IMMUNITY 0019-9567 73 12 8050 8059 20051200 10.1128/IAI.73.12.8050-8059.2005 read0046145/misc/23174362 HH Chou H Yumoto M Davey Y Takahashi T Miyamoto FC Gibson CA Genco Porphyromonas gingivalis fimbria-dependent activation of inflammatory genes in human aortic endothelial cells INFECTION AND IMMUNITY 0019-9567 73 9 5367 5378 20050900 10.1128/IAI.73.9.5367-5378.2005 read0046145/misc/23174360 A Wilensky Y Gabet H Yumoto Y Houri-Haddad L Shapira Three-dimensional quantification of alveolar bone loss in Porphyromonas gingivalis-infected mice using micro-computed tomography JOURNAL OF PERIODONTOLOGY 0022-3492 76 8 1282 1286 20050800 10.1902/jop.2005.76.8.1282 read0046145/misc/23174361 H Azakami H Akimichi M Usui H Yumoto S Ebisu A Kato Isolation and characterization of a plasmid DNA from periodontopathogenic bacterium, Eikenella corrodens 1073, which affects pilus formation and colony morphology GENE 0378-1119 351 143 148 20050500 10.1016/j.gene.2005.03.015 15869847 read0046145/misc/23174359 FC Gibson C Hong HH Chou H Yumoto JQ Chen E Lien J Wong CA Genco Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice CIRCULATION 0009-7322 109 22 2801 2806 20040600 10.1161/01.CIR.0000129769.17895.F0 read0046145/misc/23174349 宮本貴成，高橋祐介，湯本浩通 抗生物質利用におけるEBM(根拠に基づく医学)を考える 日本ヘルスケア歯科研究会誌 6 1 17 22 20040000 read0046145/misc/28548493 FC Gibson C Hong J Wong H Yumoto CA Genco Immunization prevents infection-induced acceleration of atherosclerosis in ApoE-Deficient mice CIRCULATION 0009-7322 108 17 129 129 20031000 read0046145/misc/23174358 T Matsuo T Shirakami K Ozaki T Nakanishi H Yumoto S Ebisu An immunohistological study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions JOURNAL OF ENDODONTICS 0099-2399 29 3 194 200 20030300 10.1097/00004770-200303000-00008 read0046145/misc/23174357 M Yamada H Nakae H Yumoto C Shinohara S Ebisu T Matsuo N-Acetyl-D-galactosamine specific lectin of Eikenella corrodens induces intercellular adhesion molecule-1 (ICAM-1) production by human oral epithelial cells JOURNAL OF MEDICAL MICROBIOLOGY 0022-2615 51 12 1080 1089 20021200 read0046145/misc/23174356 Y Hosokawa T Nakanishi D Yamaguchi K Takahashi H Yumoto K Ozaki T Matsuo Macrophage Inflammatory Protein 3 alpha-CC chemokine receptor 6 interactions play an important role in CD4(+) T-cell accumulation in periodontal diseased tissue CLINICAL AND EXPERIMENTAL IMMUNOLOGY 0009-9104 128 3 548 554 20020600 10.1046/j.1365-2249.2002.01865.x read0046145/misc/38092853 YAMAGUCHI Daisuke 山口 大輔 HOSOKAWA Yoshitaka 細川 義隆 SHINOHARA Chihiro 篠原 千尋 YUMOTO Hiromichi 湯本 浩通 NAKANISHI Tadashi 中西 正 NAKAE Hideaki 中江 英明 MATSUO Takashi 松尾 敬志 The expression of Fractalkine and CX3CR1 in human periodontally diseased tissue P-19 歯周炎病変局所におけるFractalkineおよびCX3CR1の発現 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 44 168 168 20020325 read0046145/misc/28609527 H Yumoto K Hirota H Nagamune K Ohkura K Murakami M Yamada H Nakae Y Miyake T Matsuo Molecular analysis of histone-like protein from Streptococcus intermedius. JOURNAL OF DENTAL RESEARCH 0022-0345 81 A286 A286 20020300 read0046145/misc/28603435 M Yamada H Yumoto C Shinohara H Nakae S Ebisu T Matuso Eikenella corrodens induces endothelial cells via MAPK family pathways. JOURNAL OF DENTAL RESEARCH 0022-0345 81 A114 A114 20020300 read0046145/misc/28593048 T Nakanishi K Takahashi Y Hosokawa D Yamaguchi C Shinohara H Yumoto T Matsuo Expression of macrophage inflammatori protein-3a in human pulpitis. JOURNAL OF DENTAL RESEARCH 0022-0345 81 A44 A44 20020300 read0046145/misc/28580875 Y Hosokawa T Nakanishi D Yamaguchi H Yumoto T Matsuo LARC-CCR6 Interactions play an important role in CD4+T cell accumulation in periodontal diseased tissue. JOURNAL OF DENTAL RESEARCH 0022-0345 81 A451 A451 20020300 read0046145/misc/28574610 N Ohmura K Hirota H Yumoto K Murakami Y Kanitani T Kashiwabara Y Miyake T Ichikawa Influence of denture plaque on the pharyngeal microflora. JOURNAL OF DENTAL RESEARCH 0022-0345 81 A284 A284 20020300 read0046145/misc/34404011 山下美由紀 渡辺久恵 三木千津 石川清美 久保万友美 蓮井義則 湯本浩通 松尾敬志 歯科衛生士の卒後研修の重要性とその意義 日本歯周病学会学術大会プログラムおよび講演抄録集 0385-0110 45th null null 20020000 read0046145/misc/23174348 石川 清美 渡邊 久恵 三木 千津 久保 万友美 山下 美由紀 大森 幸子 石川 利栄子 荒木 哲也 蓮井 義則 湯本 浩通 松尾 敬志 歯周病患者に対する禁煙指導について -ニコチネルの応用― クインテッセンス出版(株) 歯科衛生士 0911-9574 26 9 77 81 20020000 read0046145/misc/23174309 H Yumoto H Nakae M Yamada K Fujinaka C Shinohara S Ebisu T Matsuo Soluble products from Eikenella corrodens stimulate oral epithelial cells to induce inflammatory mediators ORAL MICROBIOLOGY AND IMMUNOLOGY 0902-0055 16 5 296 305 20011000 10.1034/j.1399-302X.2001.016005296.x read0046145/misc/38089620 YUMOTO Hiromichi 湯本 浩通 HIROTA Katsuhiko 弘田 克彦 YAMADA Masayoshi 山田 真義 NAKAE Hideaki 中江 英明 MURAKAMI Keiji 村上 圭史 MIYAKE Yoichiro 三宅 洋一郎 MATSUO Takashi 松尾 敬志 Cloning and expression of a Streptococcus intermedius gene encoding a histone-like protein D-10 Streptococcus intermedius histone-like proteinをコードする遺伝子のクローニングと発現 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 43 161 161 20010930 read0046145/misc/38089547 HOSOKAWA Yoshitaka 細川 義隆 NAKANISHI Tadashi 中西 正 YAMAGUCHI Daisuke 山口 大輔 SHIMIZU Hirotoshi 清水 洋利 YUMOTO Hiromichi 湯本 浩通 OZAKI Kazumi 尾崎 和美 MATSUO Takashi 松尾 敬志 Expression of LARC and CCR6 in periodontal diseased tissue B-17-14 : 55 歯周炎病変局所におけるLARCおよびCCR6の発現 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 43 128 128 20010930 read0046145/misc/38098124 YAMAGUCHI Daisuke 山口 大輔 HOSOKAWA Yoshitaka 細川 義隆 SHIMIZU Hirotoshi 清水 洋利 SHINOHARA Chihiro 篠原 千尋 YUMOTO Hiromichi 湯本 浩通 NAKANISHI Tadashi 中西 正 MATSUO Takashi 松尾 敬志 Expression of IL-18 and IL-18 reseptor in human periodontally diseased tissue B-23-15 : 40 歯周炎の病変局所におけるIL-18およびIL-18レセプターの発現 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 43 141 141 20010330 read0046145/misc/34404007 三木千津 渡辺久恵 石川清美 石川利栄子 石丸毅 蓮井義則 湯本浩通 松尾敬志 歯科衛生士の海外卒後研修に参加して 歯周組織・口腔粘膜疾患と国際協力について 日本歯周病学会学術大会プログラムおよび講演抄録集 0385-0110 44th null null 20010000 read0046145/misc/34403991 久保万友美 渡辺久恵 三木千津 石川清美 石川利栄子 蓮井義則 湯本浩通 松尾敬志 在宅患者の口腔ケアにおける歯科衛生士の役割 日本歯周病学会学術大会プログラムおよび講演抄録集 0385-0110 44th null null 20010000 read0046145/misc/27157784 Takashi Matsuo Kazumi Ozaki Hiromichi Yumoto Masahiro Yoshiyama Caries-A disease of bacteria infection Caries Preventive restoration 1 8 20010000 read0046145/misc/23174347 義歯とカンジダ 医薬ジャーナル 37 10 3009 3015 20010000 read0046145/misc/23174345 歯周炎発症における歯肉上皮細胞の役割 四国歯学会雑誌 13 2 259 265 20010000 read0046145/misc/34452399 OZAKI Kazumi 尾崎 和美 YUMOTO Hiromichi 湯本 浩通 SHINOHARA Chihiro 篠原 千尋 KIMOCHI Takeshi 木持 健 MATSUO Takashi 松尾 敬志 Bacterial Identification Using 16S rDNA Extracted from Resin-Embedded Carious Dentin 象牙質齲蝕に侵入した細菌種の同定 : 電顕用樹脂包埋試料から抽出した16S rDNAを用いて 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY 0387-2343 43 156 156 20001017 read0046145/misc/38704970 YUMOTO Hiromichi 湯本 浩通 SHINOHARA Chihiro 篠原 千尋 YAMADA Masayoshi 山田 真義 NAKAE Hideaki 中江 英明 EBISU Shigeyuki 恵比須 繁之 MATSUO Takashi 松尾 敬志 Analysis of activation mechanism of human endothelial cells stimulated by conditioned media from Eikenella corrodens C-25-9 : 50 Eikenella corrodens培養上清による血管内皮細胞の活性化機序の解析 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 42 162 162 20000925 read0046145/misc/38096061 YAMADA Masayoshi 山田 真義 NAKAE Hideaki 中江 英明 YUMOTO Hiromichi 湯本 浩通 SHINOHARA Chihiro 篠原 千尋 FUJINAKA Keiko 藤中 恵子 MATSUO Takashi 松尾 敬志 Induction of ICAM-1 on gingival epithelial cells by adhesins of Eikenella corrodens A-23-13 : 10 上皮細胞のICAM-1発現誘導におけるEikenella corrodens付着因子の関与について The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 42 78 78 20000415 read0046145/misc/38701994 Usui Masakatsu 臼井 将勝 Azakami Hiroyuki 阿座上 弘行 Yumoto Hiromichi 湯本 浩通 Kato Akio 加藤 昭夫 Molecular analysis of the plasmid from periodontopathogenic bacteria Eikenella corrodens. 137 歯周病原性細菌Eikenella corrodensから分離したプラスミドの解析 日本生物工学会 日本生物工学会大会講演要旨集 12 21 21 20000000 read0046145/misc/34404003 石川清美 渡辺久恵 三木千津 久保万友美 石丸毅 石川利栄子 蓮井義則 湯本浩通 松尾敬志 歯周病患者に対する禁煙指導にいて ニコチネルの応用 日本歯周病学会学術大会プログラムおよび講演抄録集 0385-0110 43rd null null 20000000 read0046145/misc/34403995 久保万友美 渡辺久恵 三木千津 石川清美 石丸毅 石川利栄子 蓮井義則 湯本浩通 松尾敬志 はん痕性類てんぽうそうを有する患者の口腔管理 日本歯周病学会学術大会プログラムおよび講演抄録集 0385-0110 43rd null null 20000000 read0046145/misc/23174346 レンサ球菌のヒストン様蛋白質の構造解析 Bacterial Adherence研究 14 6 8 20000000 read0046145/misc/23174344 臼歯部の動揺を伴う歯周病患者への対応∼特にメインテナンスにおける歯科衛生士の役割∼ 歯科衛生士 24 11 81 85 20000000 read0046145/misc/23174343 更年期障害を有する歯周病患者の一症例 歯科衛生士 24 2 81 84 20000000 read0046145/misc/23174329 Three-dimensional structural analysis of histone-like protein from streptococci The Japanese Association for Bacterial Adherence Research 14 6 8 20000000 read0046145/misc/38090748 YUMOTO Hiromichi 湯本 浩通 NAKAE Hideaki 中江 英明 YAMADA Masayoshi 山田 真義 FUJINAKA Keiko 藤中 恵子 EBISU Shigeyuki 恵比須 繁之 MATSUO Takashi 松尾 敬志 Induction of COX-2 expression and PGE_2 production by human epithelial cells in responce to exposure to Eikenella corrodens C-19-10 : 30 Eikenella corrodensによる上皮細胞のCOX-2発現誘導とPGE_2産生 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 41 130 130 19990330 read0046145/misc/34404005 渡辺久恵 三木千津 石川清美 石丸毅 石川利栄子 蓮井義則 湯本浩通 松尾敬志 唾液分泌低下を伴う乳癌術後患者の口腔管理 日本歯周病学会学術大会プログラムおよび講演抄録集 0385-0110 42nd null null 19990000 read0046145/misc/23174354 H Yumoto H Nakae K Fujinaka S Ebisu T Matsuo Interleukin-6 (IL-6) and IL-8 are induced in human oral epithelial cells in response to exposure to periodontopathic Eikenella corrodens INFECTION AND IMMUNITY 0019-9567 67 1 384 394 19990100 read0046145/misc/23174342 口腔がん患者における歯周治療および管理の重要性 歯科衛生士 23 9 65 69 19990000 read0046145/misc/38094928 ISHIKAWA Kiyomi 石川 清美 TADA Tomoyo 多田 智代 WATANABE Hisae 渡邊 久恵 ISHIMARU Tsuyoshi 石丸 毅 ISHIKAWA Rieko 石川 利栄子 HASUI Yoshinori 蓮井 義則 YUMOTO Hiromichi 湯本 浩通 MATSUO Takashi 松尾 敬志 A case report of an adult periodontitis patient with climacteric disorder C-21-8 : 40 更年期障害を有する歯周病患者の一症例 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 40 175 175 19980915 read0046145/misc/38091350 YAMADA Masayoshi 山田 真義 NAKAE Hideaki 中江 英明 YUMOTO Hiromichi 湯本 浩通 FUJITNAKA Keiko 藤中 恵子 MATSUO Takashi 松尾 敬志 Expression of ICAM-1 by human oral epithelium after Eikenella corrodens infection in vitro B-11-10 : 50 Eikenella corrodensによる上皮細胞のICAM-1発現誘導 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 40 106 106 19980915 read0046145/misc/38094713 YUMOTO Hiromichi 湯本 浩通 NAKAE Hideaki 中江 英明 FUJINAKA Keiko 藤中 恵子 EBISU Shigeyuki 恵比須 繁之 MATSUO Takashi 松尾 敬志 Induction of cytokine expression by human epithelial cells in response to exposure to Eikenella corrodens : Effects of different patterns of bacterial exposure B-8-10 : 36 Eikenella corrodensによる上皮細胞のサイトカイン発現誘導 : 刺激様式の差異による影響 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 40 110 110 19980415 read0046145/misc/38090047 WATANABE Hisae 渡邊 久恵 MIYOSHI China 三好 千奈 TADA Tomoyo 多田 智代 MITSUMORI Yasutomo 三森 康智 ISHIKAWA Rieko 石川 利栄子 HASUI Yoshinori 蓮井 義則 YUMOTO Hiromichi 湯本 浩通 MATSUO Takashi 松尾 敬志 Importance of periodontal traetment and maintenance of a patient with squamous cell carcinoma of oral floor C衛-2-14 : 42 口腔癌患者における歯周治療および管理の重要性 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 40 197 197 19980415 read0046145/misc/38701613 Azakami Hiroyuki 阿座上 弘行 Usui Masakatsu 臼井 将勝 Yumoto Hiromichi 湯本 浩通 Kato Akio 加藤 昭夫 Analysis of a plasmid from periodontopathogenic Eikenella corrodens. 1065 歯周病原性細菌Eikenella corrodensの有するプラスミドの解析 日本生物工学会 日本生物工学会大会講演要旨集 10 251 251 19980000 read0046145/misc/28480890 H Yumoto H Nakae K Fujinaka S Ebisu T Matsuo Stimulation of cytokine production in epithelial cells by Eikenella corrodens. JOURNAL OF DENTAL RESEARCH 0022-0345 77 903 903 19980000 read0046145/misc/28478088 H Nakae H Yumoto S Ebisu T Matsuo Purification of the glycoprotein on streptococci that aggregate Eikenella corrodens JOURNAL OF DENTAL RESEARCH 0022-0345 77 985 985 19980000 read0046145/misc/23174341 喫煙習慣をもつ歯周病患者への対応 (共著) 歯科衛生士 22 8 67 71 19980000 read0046145/misc/23174339 YOSHIYAMA Masahiro 吉山 昌宏 URAYAMA Akihisa 浦山 明久 SHINOHARA Chihiro 篠原 千尋 YUMOTO Hiromichi 湯本 浩通 MATSUO Takashi 松尾 敬志 X-ray Microanalysis of Resin-dentin Adhesive Interfaces X線微小分析法による象牙質接着界面の解析(共著) 日本歯科保存学雑誌 0387-2343 41 2 461 466 19980000 read0046145/misc/39367639 NAKANISHI Tadashi 中西 正 SHIMIZU Hirotoshi 清水 洋利 YUMOTO Hiromichi 湯本 浩通 NAKAE Hideaki 中江 英明 MATSUO Takashi 松尾 敬志 Diagnosis of irreversible pulpitis using pulpal blood 歯髄血液を用いた不可逆性歯髄炎の診断に関する研究 : 直接覆髄の成否と炎症メディエーターとの関連性 日本歯科保存学雑誌 0387-2343 40 139 139 19971013 read0046145/misc/38090420 YUMOTO Hiromichi 湯本 浩通 NAKAE Hideaki 中江 英明 FUJINAKA Keiko 藤中 恵子 MURAKAMI Keiji 村上 圭史 EBISU Shigeyuki 恵比須 繁之 MATSUO Takashi 松尾 敬志 Attachment of Eikenella corrodens to human epithelial cells D-31 Eikenella corrodensの上皮細胞への付着 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 39 174 174 19970905 read0046145/misc/38090396 TADA Tomoyo 多田 智代 MIYOSHI China 三好 千奈 WATANABE Hisae 渡邊 久恵 MITSUMORI Yasutomo 三森 康智 ISHIKAWA Rieko 石川 利栄子 HASUI Yoshinori 蓮井 義則 YUMOTO Hiromichi 湯本 浩通 MATSUO Takashi 松尾 敬志 Guidance to a patient with periodontitis having a habit of smoking E-1 喫煙習慣をもつ歯周病患者への対応 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 39 178 178 19970905 read0046145/misc/38708386 NAKAE Hideaki 中江 英明 YUMOTO Hiromichi 湯本 浩通 OZAKI Kazumi 尾崎 和美 MATSUO Takashi 松尾 敬志 EBISU Shigeyuki 恵比須 繁之 Coaggregation between Eikenella corrodens and oral bacteria isolated from periodontal pocket D-3 Eikenella corrodensと臨床分離細菌との共凝集 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 39 152 152 19970325 read0046145/misc/23174338 AZAKAMI Hiroyuki 阿座上 弘行 YUMOTO Hiromichi 湯本 浩通 EBISU Shigeyuki 恵比須 繁之 Mechanism of Oral Colonization by Periodontopathic Bacteria 歯周病原性細菌は口腔内にどのように定着するか新たな診断・予防法の開発に向けて (共著) 学会出版センタ- 化学と生物 0453-073X 35 12 848 853 19970000 10.1271/kagakutoseibutsu1962.35.848 read0046145/misc/23174353 H Yumoto H Azakami H Nakae T Matsuo S Ebisu Cloning, sequencing and expression of an Eikenella corrodens gene encoding a component protein of the lectin-like adhesin complex GENE 0378-1119 183 1-2 115 121 19961200 10.1016/S0378-1119(96)00522-7 8996095 read0046145/misc/23174352 H Azakami H Yumoto H Nakae T Matsuo S Ebisu Molecular analysis of the gene encoding a protein component of the Eikenella corrodens adhesin complex that is close to the carbohydrate recognition domain GENE 0378-1119 180 1-2 207 212 19961100 10.1016/S0378-1119(96)00639-7 8973368 read0046145/misc/34452404 YOSHIYAMA Masahiro 吉山 昌宏 SHINOHARA Chihiro 篠原 千尋 YUMOTO Hiromichi 湯本 浩通 MATSUO Takashi 松尾 敬志 X-ray Microanalysis of Resin-dentin Adhesive Interfaces X線微小分析法による象牙質接着界面の解析 日本歯科保存学雑誌 0387-2343 39 143 143 19961001 read0046145/misc/28207231 H Yumoto H Azakami H Nakae S Ebisu Molecular biological analysis of adhesin component protein from Eikenella corrodens JOURNAL OF DENTAL RESEARCH 0022-0345 75 1494 1494 19960000 read0046145/misc/23174340 歯周病原性細菌┣DBEikella(/)-┫DB ┣DBcorrodens(/)-┫DBの付着因子複合体を構成する蛋白質の分子生物学的解析 四国歯学会雑誌 9 1 19 42 19960000 read0046145/misc/38093450 AZAKAMI Hiroyuki 阿座上 弘行 YUMOTO Hiromichi 湯本 浩通 NAKAE Hideaki 中江 英明 EBISU Shigeyuki 恵比須 繁之 Gene expression and characterization of the component proteins of the Eikenella corrodens adhesin complex in Escherichia coli B-50-13 : 30 Eikenella corrodensの付着因子複合体を構成する蛋白質の発現とその解析 The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 37 140 140 19950906 read0046145/misc/38094507 YUMOTO Hiromichi 湯本 浩通 AZAKAMI Hiroyuki 阿座上 弘行 NAKAE Hideaki 中江 英明 EBISU Shigeyuki 恵比須 繁之 Molecular biological analysis of Eikenella corrodens adhesin : Cloning of the genes encoding the component proteins of the adhesin complex B-6-10 : 00 Eikenella corrodensの付着因子の分子生物学的解析 : 付着因子複合体構成蛋白遺伝子のクローニング The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 37 108 108 19950415 read0046145/misc/38089245 Nakae Hideaki 中江 英明 Yumoto Hiromiti 湯本 浩通 Hirao Kiyosi 平尾 清司 Ebisu Shigeyuki 恵比須 繁之 Purification of receptors on plaque bacteria which were recognized by Eikenella corrodens adhesin B-14 Eikenella corrodensの付着因子が認識するプラーク細菌の表層レセプター The Japanese Society of Periodontology 特定非営利活動法人日本歯周病学会 Journal of the Japanese Association of Periodontology 日本歯周病学会会誌 0385-0110 36 108 108 19940325 read0046145/misc/23174351 H NAKAE H YUMOTO T MATSUO S EBISU MITOGENIC STIMULATION OF MURINE B-LYMPHOCYTES BY THE N-ACETYL-D-GALACTOSAMINE SPECIFIC BACTERIAL LECTIN-LIKE SUBSTANCE FROM EIKENELLA-CORRODENS FEMS MICROBIOLOGY LETTERS 0378-1097 116 3 349 353 19940300 10.1016/0378-1097(94)90058-2 8181703 三木康楽賞 口腔Biofilm感染症の病因・病態解析と診断・治療法の開発 20190200 若野洋一歯科臨床医学奨励賞 19980000 三木康楽賞 19960000