Tokushima University / Educator and Researcher Directory --- Ueno, Satoru

Personal Web Page

https://researchmap.jp/read0088443 (researchmap)

Field of Study

○	Biophysical Chemistry
○	製剤物理化学

Subject of Study

○	高分子化合物の膜透過に関する研究, 生体膜に対する膜作用性薬物の作用メカニズムの研究
○	高分子電解質複合体に関する研究, DNA-脂質二分子膜相互作用に関する研究

Book / Paper

Book:

1.	Saburo Shimabayashi and Satoru Ueno : 辻彰編集 ''新薬剤学(改訂第2版)'' 第Ⅲ部物理薬剤学第4章界面化学担当, Nankodo, Tokyo, Apr. 2007. (Summary) 2002年出版の旧版を書き換え，時代に即応した新しい内容の界面科学とした．前回は嶋林の単独執筆であったのを，今回は植野と嶋林の協同執筆とした．製剤学的内容を重視するとともに，基礎分野としての界面化学およびコロイド化学の内容にも力点を置いた．薬学教育の立場から，日本薬局方および薬剤師国家試験問題も意識しつつ原稿を作成した．
2.	Saburo Shimabayashi, Hideji TANAKA and Satoru Ueno : 製剤への物理化学, 株式会社廣川書店, Tokyo, Aug. 2006. (Summary) ( ) ( ) (Keyword) physical chemistry / 製剤学 / 日本薬局方 / 初心者向き教科書

Academic Paper (Judged Full Paper):

1.	Satoru Ueno : EFFECT OF TRYPTOPHAN RESIDUES ON ADSORPTION OF A CATIONIC ARGININE RICH POLYPEPTIDE BY HYDROXYAPATITE NANOPARTICLES AND FOLLOWING TRANSLOCATION OF THE POLYPEPTIDE THROUGH A LIPID VESICLE MEMBRANE, Phosphorus Research Bulletin, Vol.25, No.1, 39-44, 2011. (Link to Publication Site) ● Publication site (DOI): 10.3363/prb.25.39 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3363/prb.25.39 (DOI: 10.3363/prb.25.39)
2.	Satoru Ueno : Formation of Poly-L-lysine - hydroxyapatite Nanoparticle Complexs and Interactions between These Complexes and Lipid Bilayer Membranes, Phosphorus Research Bulletin, Vol.24, No.1, 32-37, 2010. (Link to Publication Site) ● Publication site (DOI): 10.3363/prb.24.32 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3363/prb.24.32 (DOI: 10.3363/prb.24.32)
3.	Satoru Ueno and Saburo Shimabayashi : Poly-L-arginine-Hydroxyapatite Nanoparticle Complexes Translocate Through Lipidbilayer Membranes, Bio-Medical Materials and Engineering, Vol.19, No.2-3, 111-119, 2009. (Summary) Certain molecules, which are able to directly translocate across phospholipid bilayer membranes (cell or endosormal membrane), can be useful as carriers (vectors) for drags (especially polymeric drags). We have studied the translocationability of the hydroxyapatite nanoparticle-poly-L-arginine complex through the negatively charged phospholipid bilayer membranes by using several instruments. It was confirmed by means of a confocal laser scanning microscopy (CLSM) not only the fact that the complex can translocate through the membranes but also the fact that the complexes were still retained in the inner water layer of the liposome even after the translocation. (Keyword) Diffusion / Drug Carriers / Durapatite / Lipid Bilayers / Materials Testing / Nanoparticles / Particle Size / Peptides (Link to Publication Site) ● Publication site (DOI): 10.3233/BME-2009-0570 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19581704 ● Search Scopus @ Elsevier (PMID): 19581704 ● Search Scopus @ Elsevier (DOI): 10.3233/BME-2009-0570 (DOI: 10.3233/BME-2009-0570, PubMed: 19581704)
4.	Satoru Ueno and Saburo Shimabayashi : Formation of Poly-L-Arginine - Hydroxyapatite Nanoparticle Complexes and Interactions between Complexes and Lipidbilayer Membranes, Phosphorus Research Bulletin, Vol.23, No.1, 57-62, 2009. (Link to Publication Site) ● Publication site (DOI): 10.3363/prb.23.57 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3363/prb.23.57 (DOI: 10.3363/prb.23.57)
5.	Takeshi Murata, Satoru Ueno, Tomoaki Hino and Saburo Shimabayashi : Solubility of monoalkyl phosphate in water in the presence of arginine and Triton, and solubilization of methyl yellow through the mixed micelle, Phosphorus Research Bulletin, Vol.22, No.1, 41-47, 2008. (Summary) モノアルキルリン酸およびヂセチルリン酸は水には殆ど溶解しない．70℃の高温でTriton-XあるいはTriton-Nの存在で前者はやや溶解度が上がるが，後者はそれでも殆ど溶解しないことを指摘した．アルギニンが存在すると室温でも溶解することを確認したのち，モノアルキルリン酸ーTXあるいはTN-アルギニン混合ミセルによるメチルイエロウの可溶化量を測定した．モノアルキルリン酸の溶解機構を検討し，KOHで中和する場合とアルギニンで中和する場合の相違点について考察した． (Keyword) monoalkylphosphate / hydroxyapatite / Triton / mixed micelle / Solubilization of Methyl Yellow (Link to Publication Site) ● Publication site (DOI): 10.3363/prb.22.41 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3363/prb.22.41 (DOI: 10.3363/prb.22.41)
6.	Emiko Motoki, Satoru Ueno and Saburo Shimabayashi : Effects of ionic surfactants on the adsorption of bovine serum albumin to the surface of hydroxyapatite, Phosphorus Research Bulletin, Vol.21, No.1, 9-15, 2007. (Keyword) BSA / HAP / SDS / DTMAC (Link to Publication Site) ● Publication site (DOI): 10.3363/prb.21.9 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3363/prb.21.9 (DOI: 10.3363/prb.21.9)
7.	Satoru Ueno, Yasuko Yoshioka and Saburo Shimabayashi : Effects of Phosphate Ions on the Polypeptide-Liposome Membrane Interaction, Phosphorus Research Bulletin, Vol.21, No.1, 71-77, 2007. (Link to Publication Site) ● Publication site (DOI): 10.3363/prb.21.71 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3363/prb.21.71 (DOI: 10.3363/prb.21.71)
8.	Satoru Ueno, Saburo Shimabayashi and Akira Shibata : Polyethyleneimine can permeate through a lipid bilayer of a giant liposome, Journal of Japanese Medical Society for Biological Interface, Vol.37, No.1, 19-27, 2006. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1574231875095547520 (CiNii: 1574231875095547520)
9.	Saburo Shimabayashi, Satoru Ueno, Tomoaki Hino and Emiko Motoki : Effect of Dodecyltrimethylammonium Chloride on the Adsorption of Bovine Serum Albumin to the Surface of Hydroxyapatite, Phosphorus Research Bulletin, Vol.20, No.1, 135-140, 2006. (Keyword) hydroxyapatite / bovine serum albumin / adsorption / protein denaturation / dodecyltrimethylammonium chloride (Link to Publication Site) ● Publication site (DOI): 10.3363/prb.20.135 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3363/prb.20.135 (DOI: 10.3363/prb.20.135)
10.	Saburo Shimabayashi, Satoru Ueno, Tomoaki Hino and Kuniomi Warabino : Preparation and adsorption properties of hydroxyapatite surface-modified by cetylphosphate, Phosphorus Research Bulletin, Vol.20, No.1, 129-134, 2006. (Summary) セチル燐酸共存でヒドロキシアパタイトを合成した．その表面は疎水化されており有機化合物を容易に吸着するようになった． (Keyword) hydroxyapatite / cetylphosphate / surface modification / surface denaturation (Link to Publication Site) ● Publication site (DOI): 10.3363/prb.20.129 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3363/prb.20.129 (DOI: 10.3363/prb.20.129)
11.	Liu Shaoqian, Akira Shibata, Satoru Ueno, Huang Ying, Wang Yifan and Li Yuanjian : Translocation of positively charged copoly(Lys/Tyr) across phospholipid membranes, Biochemical and Biophysical Research Communications, Vol.339, No.3, 761-768, 2006. (Summary) Much attention has recently been paid to the study of positively charged polypeptides as a possible carrier for therapeutic protein or DNA delivery to cells. In this study, we have investigated the translocation of positively charged copoly(Lys/Tyr) (MW=72000, DP=385) across lipid membranes constituted from egg-phosphatidylcholine (EPC), dioleoyl-phosphatidylethanolamine (DOPE), as well as soybean phospholipids (SBPL) using zeta potential method, circular dichroism spectroscopy (CD), electrophysiology technique, fluorescence spectroscopy, and confocal laser scanning microscopy. Results of zeta potentials show that copoly(Lys/Tyr) associate with lipid membranes and become gradually saturated on the membranes either hydrophobically or electrostatically or both. CD studies demonstrate that the copoly(Lys/Tyr) takes and remains beta-sheet conformation during its interaction with liposome membranes, indicating that the translocation process should be carpet-mode like. Data from the electrophysiology technique reveal that positively charged copoly(Lys/Tyr) can cause transmembrane currents under an applied voltage, confirming its transfer across lipid membranes. Fluorescence spectroscopy results display a three-step mechanism of translocation across membrane: adsorption, transportation, and desorption, which has been verified by results from confocal laser scanning microscopy. We provided the first direct observation that the positively charged polypeptides, copoly(Lys/Tyr), can translocate through SBPL and EPC/DOPE lipid bilayer membranes. In addition, we found that the translocation efficiency of copoly(Lys/Tyr) was higher on the EPC/DOPE lipid membrane than on the SBPL lipid membrane. (Keyword) Electromagnetic Fields / Electroporation / Lipid Bilayers / liposomes / Membrane Fluidity / Membrane Lipids / Membranes, Artificial / Motion / Peptides / Phospholipids / Static Electricity (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbrc.2005.11.079 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16316626 ● Summary page in Scopus @ Elsevier: 2-s2.0-28844508593 (DOI: 10.1016/j.bbrc.2005.11.079, PubMed: 16316626, Elsevier: Scopus)
12.	A. Shibata, K. Maeda, H. Ikema, Satoru Ueno, Y. Suezaki, S. Liu, Y. Baba and I. Ueda : Local anesthetics facilitate ion transport across lipid planar bilayer membranes under an electric field: Dependence on type of lipid bilayer, Colloids and Surfaces B:Biointerfaces, Vol.42, No.3-4, 197-203, 2005. (Summary) In order to elucidate the role of structural change of lipid membrane bilayer in the mode of action of local anesthetic, we studied the effects of local anesthetics, charged tetracaine and uncharged benzocaine, on ion permeability across various lipid planar bilayers (PC, mixed PC/PS (4/1, mol/mol); mixed PC/PE (1/1, mol/mol); mixed PC/SM (4/1, mol/mol)) under a constant applied voltage. The membrane conductances increased in the order of PC<PC/PS<or=PC/SM<PC/PE. When the constant voltage of -100 or -70 mV was applied through the lipid bilayer membranes in the presence of positively charged tetracaine, the fluctuating current pulses with the large amplitude generated, but not appeared in the absence of tetracaine. The addition of uncharged benzocaine generated the fluctuating currents with the small amplitude. Both charged tetracaine and uncharged benzocaine facilitated electrophoretically the transport of small ions such as KCl in the buffer solution through the fluctuating pores in the lipid bilayer membranes formed by interaction with the local anesthetic under the negative applied membrane potential. The current pulses also contained actual transport of charged tetracaine together with the transport of the small ions. The amplitude and the duration time of the electrical current generated by adding the local anesthetics were dependent on the type of the lipid, the applied voltage and its voltage polarity. (Keyword) Anesthetics, Local / Benzocaine / Electromagnetic Fields / Lipid Bilayers / Membrane Potentials / Membranes, Artificial / Phospholipids / Tetracaine (Link to Publication Site) ● Publication site (DOI): 10.1016/j.colsurfb.2005.02.006 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15893219 ● Search Scopus @ Elsevier (PMID): 15893219 ● Search Scopus @ Elsevier (DOI): 10.1016/j.colsurfb.2005.02.006 (DOI: 10.1016/j.colsurfb.2005.02.006, PubMed: 15893219)
13.	Akira Shibata, Ayako Sakata, Satoru Ueno, Tsuguhide Hori, Kazushi Minami, Yoshinobu Baba and Naoki Kamo : Regeneration and inhibition of proton pumping activity of bacteriorhodopsin blue membrane by cationic amine anesthetics, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1669, No.1, 17-25, 2005. (Summary) Bacteriorhodopsin (bR) is the prototype of an integral membrane protein with seven membrane-spanning alpha-helices and serves as a model of the G-protein-coupled drug receptors. This study is aimed at reaching a greater understanding of the role of amine local anesthetic cations on the proton transport in the bR protein, and furthermore, the functional role of "the cation" in the proton pumping mechanism. The effect of the amine anesthetic cations on the proton pump in the bR blue membrane was compared with those by divalent (Ca2+, Mg2+ and Mn2+) and monovalent metal cations (Li+, Na+, K+ and Cs+), which are essential for the correct functioning of the proton pumping of the bR protein. The results suggest that the interacting site of the divalent cation to the bR membrane may differ from that of the monovalent metal cation. The electric current profile of the bR blue membrane in the presence of the amine anesthetic cations was biphasic, involving the generation and inhibition of the proton pumping activity in a concentration-dependent manner. The extent of the regeneration of the proton pump by the additives increased in the order of monovalent metal cation<monovalent amine anesthetic cation<divalent metal cation. We found that organic cations such as the amine anesthetics can also regenerate the proton pump in the bR protein. The inhibition of proton transport in the bR protein by the anesthetic cations was elucidated using the wild type, the E204Q and the D96N mutated bRs. The hydrophobic interaction of the amine anesthetics with the bR protein plays an important part in inhibiting the bR proton pump. (Keyword) Amines / Anesthetics / Bacteriorhodopsins / Cations / Cell Membrane / Halobacterium salinarum / Proton Pumps / Protons / Purple Membrane (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbamem.2005.02.002 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15842995 ● Search Scopus @ Elsevier (PMID): 15842995 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2005.02.002 (DOI: 10.1016/j.bbamem.2005.02.002, PubMed: 15842995)
14.	Kenji Fukuzawa, Yasuaki Saitoh, Kaori Akai, Kentaro Kogure, Satoru Ueno, Akira Tokumura, Masaki Otagiri and Akira Shibata : Antioxidant effect of bovine serum albumin on membrane lipid peroxidation induced by iron chelate and superoxide, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1668, No.1, 145-155, 2005. (Summary) Albumin is supposed to be the major antioxidant circulating in blood. This study examined the prevention of membrane lipid peroxidation by bovine serum albumin (BSA). Lipid peroxidation was induced by the exposing of enzymatically generated superoxide radicals to egg yolk phosphatidylcholine liposomes incorporating lipids with different charges in the presence of chelated iron catalysts. We used three kinds of Fe3+-chelates, which initiated reactions that were dependent on membrane charge: Fe3+-EDTA and Fe3+-EGTA catalyzed peroxidation in positively and negatively charged liposomes, respectively, and Fe3+-NTA, a renal carcinogen, catalyzed the reaction in liposomes of either charge. Fe3+-chelates initiated more lipid peroxidation in liposomes with increased zeta potentials, followed by an increase of their availability for the initiation of the reaction at the membrane surface. BSA inhibits lipid peroxidation by preventing the interaction of iron chelate with membranes, followed by a decrease of its availability in a charge-dependent manner depending on the iron-chelate concentration: one is accompanied and the other is unaccompanied by a change in the membrane charge. The inhibitory effect of BSA in the former at high concentrations of iron chelate would be attributed to its electrostatic binding with oppositely charged membranes. The inhibitory effect in the latter at low concentrations of iron chelate would be caused by BSA binding with iron chelates and keeping them away from membrane surface where lipid peroxidation is initiated. Although these results warrant further in vivo investigation, it was concluded that BSA inhibits membrane lipid peroxidation by decreasing the availability of iron for the initiation of membrane lipid peroxidation, in addition to trapping active oxygens and free radicals. (Keyword) Animals / Antioxidants / Cattle / Iron Chelating Agents / Lipid Peroxidation / Membrane Lipids / Peptides / Serum Albumin, Bovine / Superoxides (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbamem.2004.12.006 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15670740 ● Search Scopus @ Elsevier (PMID): 15670740 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2004.12.006 (DOI: 10.1016/j.bbamem.2004.12.006, PubMed: 15670740)
15.	Satoru Ueno, 池間秀之, 末崎幸生, 馬場嘉信 and 柴田瑩 : The local anesthetics facilitate the transport of small ions across phospholipid bilayer, Anesthesia and Resuscitation, Vol.41, No.1, 9-12, 2005. (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-71249101691 (Elsevier: Scopus)
16.	Kaori Akai, Koichiro Tsuchiya, Akira Tokumura, Kentaro Kogure, Satoru Ueno, Akira Shibata, Toshiaki Tamaki and Kenji Fukuzawa : Ability of ferric nitrilotriacetate complex with three pH-dependent conformations to induce lipid peroxidation., Free Radical Research, Vol.38, No.9, 951-962, 2004. (Link to Publication Site) ● Publication site (DOI): 10.1080/1071576042000261945 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15621713 ● Search Scopus @ Elsevier (PMID): 15621713 ● Search Scopus @ Elsevier (DOI): 10.1080/1071576042000261945 (DOI: 10.1080/1071576042000261945, PubMed: 15621713)
17.	Toyoshi Hasegawa, Atsushi Bando, Koichiro Tsuchiya, Shinji Abe, Masumi Okamoto, Kazuyoshi Kirima, Satoru Ueno, Masanori Yoshizumi, Hitoshi Houchi and Toshiaki Tamaki : Enzymatic and non-enzymatic formation of reactive oxygen species from 6-anilino-5,8-quinolineequinone, Biochimica et Biophysica Acta (BBA) - General Subjects, Vol.1670, No.1, 19-27, 2004. (Summary) The nonenzymatic and enzymatic formation of reactive oxygen species (ROS) from LY83583 (6-anilino-5,8-quinolinequinone) was investigated by electron paramagnetic resonance (EPR) spectroscopy. In the presence of thiol compounds such as glutathione and L-cysteine, LY83583 underwent a one-electron reduction due to low redox potential (-0.3+/-0.01 V vs. SCE), followed by formation of LY83583 semiquinone anion radical. This species was characterized by EPR spectroscopy under an argon atmosphere at neutral pH. Under an aerobic condition, this species interacts with molecular oxygen to form a superoxide anion radical. GSH-conjugated LY83583 was also identified by NMR and FAB-MS. When LY83583 was applied to PC12 cells, ROS formation was completely inhibited by both the flavoenzyme inhibitor DPI and the DT-diaphorase inhibitor dicumarol. On the other hand, ROS generation occurred independent of intracellular GSH level. These results indicate that LY83583 can generate ROS both enzymatically and nonenzymatically, although the enzymatic formation is dominant over the nonenzymatic system in PC12 cells. (Keyword) Aminoquinolines / Animals / Chromatography, High Pressure Liquid / Cyclic N-Oxides / Electrochemistry / Electron Spin Resonance Spectroscopy / Enzyme Inhibitors / Glutathione / Glutathione Disulfide / Magnetic Resonance Spectroscopy / Mass Spectrometry / Molecular Structure / Oxygen Consumption / PC12 Cells / Rats / Reactive Oxygen Species / Spin Trapping (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbagen.2003.10.008 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14729138 ● Search Scopus @ Elsevier (PMID): 14729138 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbagen.2003.10.008 (DOI: 10.1016/j.bbagen.2003.10.008, PubMed: 14729138)
18.	Akira Shibata, Syuichi Murata, Satoru Ueno, Shaoqiqn Liu, Shiroh Futaki and Yoshinobu Baba : Synthetic copoly(Lys/Phe) and poly(Lys) translocate through lipid bilayer membranes, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1616, No.2, 147-155, 2003. (Summary) Several membrane-transporting peptides (MTP) containing basic amino acid residues such as Lys and Arg that carry macromolecules such as DNA and proteins across cell plasma membranes by an unknown mechanism have been actively studied. On the basis of these results, we have been investigating the translocation ability of synthetic polypeptides, copoly(Lys/Phe) and poly(Lys), through negatively charged phospholipid (soybean phospholipid (SBPL)) bilayer membranes by zeta potential analysis, circular dichroism (CD) spectroscopy, fluorescence spectroscopy, an electrophysiology technique, and confocal laser scanning microscopy (CLSM). The binding of these polypeptides to the membrane, which is the first step for translocation across the membrane, resulted in the conformational transition of the polypeptide from a random coil form or helix-poor form to a helix-rich form. The fluorescence studies demonstrated that the time-dependent decrease in the fluorescence intensities of the FITC-labeled polypeptides bound to the SBPL liposome reflected translocation of the polypeptide across the lipid bilayer with the low dielectric constant. Both the rate constant and the efficiency of the polypeptide translocation across the lipid bilayer were greater for copoly(Lys/Phe) than for poly(Lys). These results suggest that the random incorporation of the hydrophobic Phe residue into the positively charged Lys chain results in a lowering of the potential barrier for passage of the polypeptide in the hydrophobic core portion of the lipid bilayer. We presented the first direct observation that the positively charged polypeptides, copoly(Lys/Phe) (MW: 41,500) and poly(Lys) (MW: 23,400), could translocate across the lipid bilayer membrane. (Keyword) Positively charged polypeptide / Lipid membrane / Translocation / Secondary structure / Micropolarity (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbamem.2003.08.003 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14561472 ● Search Scopus @ Elsevier (PMID): 14561472 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2003.08.003 (DOI: 10.1016/j.bbamem.2003.08.003, PubMed: 14561472)
19.	Satoru Ueno, Akira Shibata, Yorimitsu Ayako, Yoshinobu Baba and Naoki Kamo : Redox potentials of the oriented film of the wild-type, the E194Q-, E204Q- and D96N-mutated bacteriorhodopsin, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1609, No.1, 109-114, 2003. (Summary) The redox potentials of the oriented films of the wild-type, the E194Q-, E204Q- and D96N-mutated bacteriorhodopsins (bR), prepared by adsorbing purple membrane (PM) sheets or its mutant on a Pt electrode, have been examined. The redox potentials (V) of the wild-type bR were -470 mV for the 13-cis configuration of the retinal Shiff base in bR and -757 mV for the all-trans configuration in H(2)O, and -433 mV for the 13-cis configuration and -742 mV for the all-trans configuration in D(2)O. The solvent isotope effect (DeltaV=V(D(2)O)-V(H(2)O)), which shifts the redox potential to a higher value, originates from the cooperative rearrangements of the extensively hydrogen-bonded water molecules around the protonated C=N part in the retinal Schiff base. The redox potential of bR was much higher for the 13-cis configuration than that for the all-trans configuration. The redox potentials for the E194Q mutant in the extracellular region were -507 mV for the 13-cis configuration and -788 mV for the all-trans configuration; and for the E204Q mutant they were -491 mV for the 13-cis configuration and -769 mV for the all-trans configuration. Replacement of the Glu(194) or Glu(204) residues by Gln weakened the electron withdrawing interaction to the protonated C=N bond in the retinal Schiff base. The E204 residue is less linked with the hydrogen-bonded network of the proton release pathway compared with E194. The redox potentials of the D96N mutant in the cytoplasmic region were -471 mV for the 13-cis configuration and -760 mV for the all-trans configuration which were virtually the same as those of the wild-type bR, indicating that the D to N point mutation of the 96 residue had no influence on the interaction between the D96 residue and the C=N part in the Schiff base under the light-adapted condition. The results suggest that the redox potential of bR is closely correlated to the hydrogen-bonded network spanning from the retinal Schiff base to the extracellular surface of bR in the proton transfer pathway. (Keyword) Redox potential / Bacteriorhodopsin / Mutant / Shiff base / Hydrogen-bonded network (Link to Publication Site) ● Publication site (DOI): 10.1016/S0005-2736(02)00660-0 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12507765 ● Search Scopus @ Elsevier (PMID): 12507765 ● Search Scopus @ Elsevier (DOI): 10.1016/S0005-2736(02)00660-0 (DOI: 10.1016/S0005-2736(02)00660-0, PubMed: 12507765)
20.	Satoru Ueno and Akira Shibata : Redox potential of bacteriorhodopsin in oriented purple membrane films, Colloids and Surfaces B:Biointerfaces, Vol.23, No.4, 383-390, 2002. (Keyword) Bacteriorhodopsin / Langmuir Blodgett technique / Electrophoretic sedimentation technique / Voltammetry / Redox potential (Link to Publication Site) ● Publication site (DOI): 10.1016/S0927-7765(01)00259-4 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/S0927-7765(01)00259-4 (DOI: 10.1016/S0927-7765(01)00259-4)
21.	Akira Shibata, A. Yorimitsu, H. Ikema, K. Minami, Satoru Ueno, E. Muneyuki and Tomihiko Higuti : Photocurrent of purple membrane adsorbed onto a thin polymer film, --- action characteristics of the local anesthetics ---, Colloids and Surfaces B:Biointerfaces, Vol.23, No.4, 375-382, 2002. (Keyword) Oriented purple membrane / Photocurrent / Local anesthetic / Binding site (Link to Publication Site) ● Publication site (DOI): 10.1016/S0927-7765(01)00272-7 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/S0927-7765(01)00272-7 (DOI: 10.1016/S0927-7765(01)00272-7)
22.	Satoru Ueno, 頼光絢子, 馬場嘉信 and 柴田瑩 : 膜蛋白質のプロトンポンプ活性に対するアミン形局所麻酔薬の二相性効果, Anesthesia and Resuscitation, Vol.38, 195-197, 2002.
23.	Akira Shibata, H. Ikema, Satoru Ueno, E. Muneyuki and Tomihiko Higuti : Alkane derivative-bacteriorhodopsin interaction: proton transport and protein structure, Colloids and Surfaces B:Biointerfaces, Vol.22, No.1, 31-38, 2001. (Summary) The effects of alkane derivatives, 1-alcohols (ROH), aliphatic amine hydrochlorides (RNH(2).HCl) and sodium aliphatic carboxylates (ROONa), on the proton pumping activity of bacteriorhodopsin (bR) in a purple membrane have been examined. Photocurrents in bR in the purple membrane adsorbed onto polyester thin film were recorded before and after exposure to these test substances. The peak photocurrent in bR was reversibly suppressed by each substance. From the dose-response curve, the concentrations required to reduce the peak capacitive current by 50% were determined for each homolog and then the standard free energies per CH(2) for the adsorption of the alkane derivatives to the site of action were estimated: -3.13 kJ mol(-1) for ROH, -3.05 kJ mol(-1) for RNH(3)(+), and -2.95 kJ mol(-1) for ROO(-). The proton pumping activity of bR was mainly suppressed by the hydrophobic interaction with the additive. The relative potencies of the functional groups of the alkane derivatives were almost comparable between 1-octanol (C(8)OH) and octylamine hydrochloride (C(8)NH(3)(+)) and about 10 times less effective for sodium octanoate (C(8)OO(-)) than for others. The addition of C(8)OH or C(8)OO(-) changed the absorption spectra of bR with a maximum at 560 nm to the spectra of the intermediate state with a maximum at 480 nm, while the C(8)NH(3)(+) decreased the intensity of the 560 nm band only with no blue-shift by the 480 nm band. We conclude that the action of the alkane derivatives is nonspecific and directed to all organized purple membrane structures and that the binding sites of the ROH and ROO(-) are different from that of RNH(3)(+). (Keyword) Bacteriorhodopsin / Photocurrent / Alkane derivatives / Fluorescence spectrum / Absorption spectrum (Link to Publication Site) ● Publication site (DOI): 10.1016/S0927-7765(01)00155-2 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11438238 ● Summary page in Scopus @ Elsevier: 2-s2.0-0034955467 (DOI: 10.1016/S0927-7765(01)00155-2, PubMed: 11438238, Elsevier: Scopus)
24.	Kenji Fukuzawa, Akira Tokumura, Kentaro Kogure, Masahito Iemura, Naoto Gondoh, Masanobu Fujii, Satoru Ueno and Akira Shibata : A comparative study of the ability of ferric nitrilotriacetate and other iron chelators to assist membrane lipid peroxidation by superoxide radicals, Chemistry and Physics of Lipids, Vol.110, No.1, 69-84, 2001. (Summary) This study examined some of the variables determining the efficiency of lipid peroxidation in egg yolk phosphatidylcholine liposomes and in microsomes exposed to enzymatically-generated superoxide radicals. The initiation of peroxidation required the presence of preformed lipid peroxides and a chelated metal catalyst. Comparison of the relative effectiveness of four iron chelating agents showed that the chelate must bind to the membrane by coulombic attraction between the charged membrane and a chelate carrying an opposite net charge. Of the chelates tested, only the carcinogenic ferric nitrilotriacetate [corrected] (Fe(3+)-NTA) was an effective catalyst of oxidation of all membranes, whether carrying a net charge, or not. We postulate that the unique catalytic capacity of the ferric nitrilotriacetate [corrected] (Fe(3+)-NTA) can be explained by its existence in two forms at neutral pH, each binding to oppositely charged membranes and initiating their peroxidation. This gives the complex the unique ability to bind to any membrane, which may be a factor in its carcinogenicity. (Keyword) Animals / Ascorbic Acid / Carcinogens / Ferric Compounds / Hydrogen-Ion Concentration / Iron Chelating Agents / Lipid Peroxidation / Liposomes / Male / Microsomes, Liver / NADP / Nitrilotriacetic Acid / Oxidation-Reduction / Oxygen / Phosphatidylcholines / Rats / Rats, Wistar / Superoxides / Thiobarbituric Acid Reactive Substances (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11245836 ● Search Scopus @ Elsevier (PMID): 11245836 (PubMed: 11245836)
25.	A. Shibata, K. Yamada, H. Ikema, Satoru Ueno, E. Muneyuki and Tomihiko Higuti : Photocurrent of purple membrane adsorbed onto a thin solid film: effects of monovalent and divalent ions, Studies in Surface Science and Catalysis, Vol.132, 635-638, 2001. (Link to Publication Site) ● Publication site (DOI): 10.1016/S0167-2991(01)82171-6 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0035787129 (DOI: 10.1016/S0167-2991(01)82171-6, Elsevier: Scopus)
26.	山田桂子, 池間秀之, Satoru Ueno and 柴田瑩 : 膜蛋白質 - 麻酔薬相互作用:界面の水の効果, Anesthesia and Resuscitation, Vol.36, 29-31, 2000.
27.	Satoru Ueno, 柴田瑩 and 樋口富彦 : 揮発性麻酔薬と紫膜の相互作用:セボフルランの作用特性, Anesthesia and Resuscitation, Vol.36, 155-158, 2000.
28.	池間秀之, 柴田瑩, Satoru Ueno, 馬場嘉信 and 樋口富彦 : 好塩菌紫膜に対する電荷型および非電荷型テトラカインの作用部位, Anesthesia and Resuscitation, Vol.35, 31-34, 1999.
29.	池間秀之, 柴田瑩, Satoru Ueno and 馬場嘉信 : バクテリオロドプシンのプロトンポンプ阻害:電荷形及び非電荷形三級アミンの効果, 膜, Vol.11, 65-68, 1999.
30.	Akira Shibata, Satoru Ueno, Yoshihiro Ito and Shinsuke Yamashita : Monolayer of Poly(L-glutamate) with an n-Dodecyl Group in the Side Chain on the Water Surface and Its Multilayers, Langmuir, Vol.14, No.26, 7519-7521, 1998.
31.	Satoru Ueno, Akira Shibata and Y. Ito : Redox potential of bacteriorhodopsin in purple membrane determined by differential pulse voltammetry, Chemical & Pharmaceutical Bulletin, Vol.46, 1944-1945, 1998.
32.	Akira Shibata, 吉田淳子, Satoru Ueno, 福澤健治, 中条信義 and 上田一作 : 揮発性麻酔薬と紫膜の相互作用における水の役剤, 麻酔と蘇生, Vol.33, 267-270, 1997.
33.	Akira Shibata, Y. Iizuka, Satoru Ueno and T. Yamashita : Effect of water activity on unfolding of adsorbed protein at the interface, Thin Solid Films, 549-551, 1996.
34.	Akira Shibata, F. Ueda, E. Okamoto, Satoru Ueno and T. Yamashita : Interaction of neutral and charged local anesthetic with purple membrane, Chemical & Pharmaceutical Bulletin, Vol.44, 621-623, 1996.
35.	Akira Shibata, S. Kawanami, K. Ushio, Satoru Ueno and T. Yamashita : Anesthetic-bacteriorhodopsin interaction;alcohol induced biphasic effects, Chemical & Pharmaceutical Bulletin, Vol.43, 1831-1835, 1995.
36.	Akira Shibata, J. Kohara, Satoru Ueno and T. Yamashita : Monovalent cations depress the unfolding of the α-helical bacteriorhodopsin at the air-water interface, Chemical & Pharmaceutical Bulletin, Vol.43, No.1-4, 1995.
37.	Akira Shibata, Y. Iizuka, Satoru Ueno and T. Yamashita : Protein surface denaturation and the surface-activated water molecules, Protein Engineering, Vol.9, 1169, 1995.
38.	Akira Shibata, J. Kohara, Satoru Ueno, I. Uchida, T. Mashimo and T. Yamashita : Monovalent anions stabilize the structure of bacteriorhodopsin at the air-water interface, Thin Solid Films, Vol.244, No.1-2, 736-739, 1994.
39.	Akira Shibata, K. Nakajima, Satoru Ueno and T. Yamashita : Preparation and characterization of liposomes incorporating hydrophobic poly(amino acid)s with different secondary structure, Chemical & Pharmaceutical Bulletin, Vol.42, 1151-1153, 1994.
40.	Akira Shibata, Ritsuko Tsukamoto, Satoru Ueno and Takuya Yamashita : Dispersion polar surface free energies of polypeptide films, Colloids and Surfaces, Vol.78, 189-192, 1993.
41.	N. Ueyama, Satoru Ueno, A. Nakamura, K. Wada, H. Matsubara, S. Kumagai, S. Sakakibara and T. Tsukihara : A synthetic analog for the active site of plant-type ferrodoxin: two different coordination isomers by a four-cys-containing [20]-peptide., Biopolymers, Vol.32, 1535-1544, 1992.
42.	Akira Shibata, Yuko Hashimura, Satoru Ueno and Takuya Yamashita : Polymerization and conformation of N-carboxy anhydrides of a L-glutamic acid derivative in monolayer, Langmuir, Vol.7, No.10, 2261-2265, 1991.
43.	Norikazu Ueyama, Satoru Ueno, Takashi Sugawara, Kazuyuki Tatsumi, Akira Nakamura and Noritake Yasuoka : Synthesis, crystal structure and electronic properties of a 2Fe-2S complex with a bulky thiolato ligand, [NEt4]2[Fe2S2(SC6H2Me3-2,4,6)4], Journal of the Chemical Society, Dalton Transactions, No.10, 2723-2727, 1991.
44.	A. Shibata, K. Arii, Satoru Ueno, Y. Suezaki and T. Yamashita : Penetration of 1-alkanols into monolayers of α-helical polypeptides with hydrophobic side chains, Colloids and Surfaces, Vol.39, 281-290, 1989.

Academic Paper (Unrefereed Paper):

1.	Kenji Fukuzawa, 権藤直人, Akira Tokumura, Satoru Ueno and 柴田螢 : スーパーオキシド依存症の脂質過酸化を誘導する発癌性鉄錯体Fe-NTA(ニトリロ三酢酸)の特性, フリーラジカルの臨床, Vol.12, 70-76, 1997.

Review, Commentary:

1.	Satoru Ueno, Akira Shibata and Saburo Shimabayashi : Polyethyleneimine can permeate the lipid bilayer of giant liposome., Journal of Japanese Medical Society for Biological Interface, Vol.37, No.1, 44, Oct. 2006. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1573105975188702336 (CiNii: 1573105975188702336)
2.	Saburo Shimabayashi and Satoru Ueno : アパタイトと生体関連物質とのマイクロ/ナノ結合機構, バイオマテリアル―生体材料―, Vol.24, No.5, 311-318, Oct. 2006.

Proceeding of International Conference:

1.	Satoru Ueno and Saburo Shimabayashi : Poly(Arg)-nanoparticle complexes translocate through lipid bilayer membranes, International Symposium on Nanotoxicology Assessment and Biomedical, Environmental Application of Fine Particles and Nanotubes (ISNT2008); Proceedings - Extended Abstracts, Vol.1, No.1, Sapporo, Jun. 2008. (Summary) ポリアミノ酸Poly(Arg)とヒドロキシアパタイトの複合体が脂質2分子膜を透過する機構を，蛍光と円二色性の測定，および共焦点顕微鏡による観察結果をもとに検討した． (Keyword) poly(Arg) / nanoparticle / lipid bilayer membrane / hydroxyapatite / translocation

Proceeding of Domestic Conference:

1.	Satoru Ueno, 許斐裕正 and 中島克 : カチオン性ポリペプチドと脂質二分子膜との相互作用, 日本薬学会第140年会(広島), Mar. 2021.
2.	Satoru Ueno : カチオン性ポリペプチドと脂質二分子膜との相互作用, 日本薬学会第139年会(千葉), Mar. 2019.
3.	Satoru Ueno : カチオン性ポリペプチドと脂質二分子膜との相互作用, 日本薬学会第137年会(仙台), Mar. 2017.
4.	Satoru Ueno : 環境感受性蛍光色素を利用したカチオン性高分子-脂質二分子膜相互作用の可視化, 日本薬学会第136年会(横浜), Mar. 2016.
5.	Satoru Ueno : 環境感受性蛍光色素 laurdanを利用した脂質膜流動性変化の可視化, 日本薬学会第135年会(神戸), Mar. 2015.
6.	Satoru Ueno : 環境感受性蛍光色素 laurdan利用した脂質二分子膜流動性変化の可視化, 日本薬学会第134年会(大分), Mar. 2014.
7.	吉井遼, 中谷亮介 and Satoru Ueno : カチオン性ポリマーによって誘起された脂質二分子膜流動性変化の可視化, 日本薬学会第133年会, Mar. 2013.
8.	武知佑樹, Haruka Yoshii, 田中将史, Satoru Ueno, 川上徹, 相本三郎 and Hiroyuki Saito : 膜透過性アルギニンポリペプチドと酸性リン脂質膜の相互作用, 日本膜学会第33年会, May 2011.
9.	Satoru Ueno and Hiroyuki Saito : 正電荷型ポリペプチド-ヒドロキシアパタイトナノパーティクル複合体形成と脂質二分子膜との相互作用に及ぼす疎水性アミノ酸残基の影響, 日本薬学会第131年会, Mar. 2011.
10.	武知佑樹, Haruka Yoshii, 田中将史, Satoru Ueno, 川上徹, 相本三郎 and Hiroyuki Saito : 細胞膜透過ペプチドの物理的膜透過メカニズム解明に向けたアルギニンポリペプチド-脂質二分子膜間相互作用の解析, 第32回生体膜と薬物の相互作用シンポジウム, Nov. 2010.
11.	Satoru Ueno and Hiroyuki Saito : 疎水性アミノ酸残基を持つカチオン性コポリペプチドと脂質二分子膜との相互作用, 膜シンポジウム2010, Nov. 2010.
12.	Satoru Ueno and Hiroyuki Saito : 疎水性アミノ酸残基を持つカチオン性ポリペプチド-ヒドロキシアパタイトナノパーティクル複合体の形成と脂質二分子膜との相互作用, 無機リン化学討論会第20回大会, Oct. 2010.
13.	Satoru Ueno and 斉藤博幸 : 疎水性アミノ酸残基を持つカチオン性ポリペプチド-ヒドロキシアパタイトナノパーティクル複合体の形成と脂質二分子膜との相互作用, 第20回無機リン化学討論会講演要旨集, Vol.20, No.1, Oct. 2010.
14.	Satoru Ueno and Hiroyuki Saito : カーボンナノチューブ-カチオン性ポリペプチド複合体の形成とその脂質との相互作用, ナノ・バイオメディカル学会第3回大会, Sep. 2010.
15.	田中宏和 and Satoru Ueno : 脂質二分子膜と Poly-L-Lysine の相互作用における Poly-L-Lysine の分子量の影響, 第48回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会講演要旨集, Vol.48, No.1, Nov. 2009.
16.	武部吏 and Satoru Ueno : ポリエチレンイミンとタンパク質との複合体形成について, 第48回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会講演要旨集, Vol.48, No.1, Nov. 2009.
17.	武知佑樹 and Satoru Ueno : poly-L-arginineの脂質二分子膜透過に与える分子量の影響, 第48回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会講演要旨集, Vol.48, No.1, Nov. 2009.
18.	Satoru Ueno : poly-L-lysine-ヒドロキシアパタイトナノパーティクル複合体の形成と脂質二分子膜との相互作用, 第19回無機リン化学討論会講演要旨集, Vol.19, No.1, Oct. 2009.
19.	Satoru Ueno : poly-L-arginine - ヒドロキシアパタイトナノパーティクル複合体と脂質二分子膜との相互作用, 第62回コロイドおよび界面化学討論会講演要旨集, Vol.62, No.1, Sep. 2009.
20.	Satoru Ueno : mwct -カチオン性ポリペプチド複合体に及ぼすポリペプチド二次構造の影響, ナノ・バイオメディカル学会講演要旨集, Vol.2, No.1, Jul. 2009.
21.	Satoru Ueno and Saburo Shimabayashi : Poly-L-arginine-ヒドロキシアパタイトナノパーテイクル複合体形成と脂質二分子膜との相互作用, 日本薬学会第129年会講演要旨集(京都, 27Q-am158), Vol.129, No.4, Mar. 2009.
22.	Satoru Ueno and Saburo Shimabayashi : Poly(Arg) - nanoparticle complexes translocate through lipid bilayer membranes., 日本生物物理学会第46回年会講演予稿集(福岡), Vol.48, No.special issue #1, S160, Dec. 2008. (Keyword) poly(Arg) / nanoparticle / lipid bilayer / cell membrane model / menbrane permeation
23.	Satoru Ueno, Saburo Shimabayashi and 山崎秀朗 : ポリエチレンイミンとリポソームの相互作用の検討, 第47回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会講演要旨集, Vol.47, No.1, Nov. 2008. (Keyword) リポソーム / ポリエチレンイミン / 脂質2分子膜 / 膜透過
24.	Satoru Ueno and Saburo Shimabayashi : Permeation of a complex of cationic polypeptide with hydroxyapatite nanoparticle through a lipid bilayer membrane, 第18回無機リン化学討論会講演要旨集(October, 2008 奈良), Vol.18, No.1, Oct. 2008. (Summary) The study on the title was done. The mechanism was discussed on the basis of the data of Zeta-potential and fluorescence spectra, and on the observation data obtained by means of a confocul microscope. (Keyword) complex between cationic polypeptide and hydroxyapatite nanopa-ticle / membrane permeation / lliposome
25.	Satoru Ueno and Saburo Shimabayashi : Cationic polypeptide-nanoparticle complexes translocate through lipid bilayer membranes., 第61回コロイドおよび界面化学討論会講演要旨集(福岡), Vol.61, No.1, Sep. 2008. (Summary) カチオン性ポリペプチド·ナノパーテイクル複合体が脂質二分子膜を透過する機構を，種々の物理化学的方法で観察し解析した． (Keyword) 膜透過 / 脂質二分子膜 / カチオン性ポリペプチド / カチオン性ポリペプチド・ナノパーテイクル複合体
26.	Satoru Ueno and Saburo Shimabayashi : カチオン性ポリペプチドによるヒドロキシアパタイトナノ粒子の脂質二分子膜透過, 第24回日本DDS学会学術集会講演要旨集(東京), Vol.24, No.1, Jun. 2008.
27.	Satoru Ueno and Saburo Shimabayashi : カチオン性ポリペプチドによるヒドロキシアパタイトナノ粒子の脂質二分子膜透過, 第24回日本DDS 学会学術集会講演要旨集(東京), Vol.24, No.1, Jun. 2008. (Keyword) Cell penetrating peptides / Hydroxyapatite nanoparticle / Membrane penetration of cationic polypeptide
28.	[ Naoyuki Uratsuji, Satoru Ueno and Saburo Shimabayashi : DNA-Liposome複合体Lipoplex形成に対する脂質構成比の影響, 第24回日本DDS学会学術集会講演要旨集(東京), Vol.24, No.1, Jun. 2008. (Keyword) DNA / リポゾーム / Lipoplex / DNA細胞導入
29.	Yuuki Tanigawa, Satoru Ueno and Saburo Shimabayashi : ポリプレックスの構造と物性に対する分子量の及ぼす影響, 第24回日本DDS 学会学術集会講演要旨集(東京), Vol.24, No.1, Jun. 2008. (Summary) ポリプレックスの構造と物性に及ぼすポリーL-リジンの分子量の影響を調べた． (Keyword) ポリプレックス / 遺伝子導入 / ポリフェクション / ポリーL-リジン / DNA
30.	Satoru Ueno and Saburo Shimabayashi : カチオン性ポリペプチドと結合したタンパク質分子やナノパーティクルの脂質二分子膜透過, 第2回日本生物物理学会四国支部大会講演要旨集, Vol.1, No.1, May 2008. (Keyword) 膜透過 / ナノパーティクル / 脂質2分子膜
31.	Satoru Ueno and Saburo Shimabayashi : 正荷電型ポリアミノ酸のリポソーム脂質2分子膜透過に対する2次構造の影響, 日本薬学会第128年会講演要旨集(横浜), Vol.128, No.4, 14, Mar. 2008.
32.	浦辻直幸, Satoru Ueno and Saburo Shimabayashi : 遺伝子導入ベクターとしてのLiposomeの構造，性質の変化に及ぼす電荷比の影響, 第46回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会(高知)講演要旨集, Vol.46, No.1, Nov. 2007.
33.	金久祥史, Satoru Ueno and Saburo Shimabayashi : プルロニックによるメチルイエローの特異的な可溶化, 第46回日本薬学会・日本薬剤師会·日本病院薬剤師会中国四国支部学術大会(高知)講演要旨集, Vol.46, No.1, Nov. 2007. (Summary) プルロニックは疎水基と親水基をもつモノマーからなる共重合体である．この高分子は，ミセルようの会合体を形成してメチルイエローを可溶化する．この可溶化量は添加した食塩量によって変化する．このNaCl 添加量依存的な可溶化の機構の解明に挑戦した．
34.	谷河佑城, Satoru Ueno and Saburo Shimabayashi : Polyplexの構造と物性に及ぼすPoly-L-lysine分子量の依存性, 第46回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会(高知)講演要旨集, Vol.46, No.1, Nov. 2007.
35.	水野智, Satoru Ueno and Saburo Shimabayashi : 水中におけるアスピリンとグルコース系高分子との相互作用, 第46回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会(高知)講演要旨集, Vol.46, No.1, Nov. 2007. (Summary) 水中でアスピリンと結晶性セルロース，デンプン，低置換度ヒドロキシプロピルセルロース等との結合性について検討．高分子の広がり，水素結合の介在する結合の重要性を指摘した．
36.	小田奈央子, 村田武史, Satoru Ueno and Saburo Shimabayashi : ヒドロキシアパタイト・水界面におけるアルキルリン酸関与の有機化合物界面複合体形成, 第17回無機リン化学討論会講演要旨集(October 2007，札幌), Vol.17, No.1, Oct. 2007. (Keyword) ヒドロキシアパタイト / 界面複合体 / 表面改質 / 界面化学
37.	Satoru Ueno and Saburo Shimabayashi : カチオン性ポリペプチドの脂質二分子膜透過, 第4回「ナノトキシコロジーアセスと微粒子・ナノチューブのバイオ応用」研究会講演要旨集, Vol.4, No.1, Aug. 2007. (Summary) 水中におけるカチオン性ポリペプチドの脂質二分子膜透過を研究．植野が研究計画立案，データ整理，口頭発表を担当．嶋林は実験結果の討論に参加．植野が主催者からの参加·発表の依頼を受けて，口頭発表．
38.	Satoru Ueno and Saburo Shimabayashi : カチオン性ポリペプチドの脂質二分子膜透過, 第1回日本生物物理学会四国支部大会講演要旨集, Vol.1, 6, Jun. 2007.
39.	Satoru Ueno and Saburo Shimabayashi : 正電荷型ポリアミノ酸とリポソーム脂質2分子膜間の相互作用に対する共存イオンの影響, 日本薬学会第127年会(富山)講演要旨集, Vol.127, No.3, 22, Mar. 2007.
40.	濱本拓也, Satoru Ueno and Saburo Shimabayashi : カチオン性リポソームとDNAの複合体形成, 日本薬学会第127年会(富山)講演要旨集, Vol.127, No.3, 22, Mar. 2007.
41.	大橋幸太, Satoru Ueno and Saburo Shimabayashi : コポリアミノ酸/蛋白質複合体のリポソーム膜との相互作用, 日本薬学会第127年会(富山)講演要旨集, Vol.127, No.3, 49, Mar. 2007.
42.	Satoru Ueno and Saburo Shimabayashi : ポリアミノ酸-リポソーム膜相互作用に対するリン酸イオンの影響, 第16回無機リン化学討論会講演要旨集, Vol.16, 78-79, Nov. 2006.
43.	Saburo Shimabayashi, Satoru Ueno and Naoko Oda : 界面活性剤混合ミセルを介したヒドロキシアパタイトへの水溶性および難水溶性色素の吸着, 第16回無機リン化学討論会講演要旨集, Vol.16, 84-85, Nov. 2006.
44.	Saburo Shimabayashi, Satoru Ueno and Emiko Motoki : ヒドロキシアパタイト表面へのウシ血清アルブミンの吸着に対するイオン性界面活性剤添加の影響, 第16回無機リン化学討論会講演要旨集, Vol.16, 86-87, Nov. 2006.
45.	Kouta Ouhashi, Satoru Ueno and Saburo Shimabayashi : コポリアミノ酸/タンパク質複合体のリポソーム膜との相互作用, 第45回日本薬学会日本病院薬剤師会中国四国支部学術大会講演要旨集, Vol.45, No.1, 28B11-20, Oct. 2006.
46.	Takuya Hamamoto, Satoru Ueno and Saburo Shimabayashi : カチオン性リポソームとDNAの複合体形成, 第45回日本薬学会日本病院薬剤師会中国四国支部学術大会講演要旨集, Vol.45, No.1, 28B11-30, Oct. 2006.
47.	Satoru Ueno and Saburo Shimabayashi : カチオン性ポリペプチドと中性リポソームとの相互作用, 第59回コロイドおよび界面化学討論会講演要旨集, Vol.59, P134, Sep. 2006.
48.	Satoru Ueno and Saburo Shimabayashi : 負電荷型ポリアミノ酸とリポソーム脂質2分子膜の相互作用, 第7回長井シンポジウム講演要旨集, Vol.7, P111, Sep. 2006.
49.	Takuya Hamamoto, Satoru Ueno and Saburo Shimabayashi : カチオン性リポソームとDNAの複合体におけるDNAの構造変化, 第7回長井シンポジウム講演要旨集, Vol.7, P107, Sep. 2006.
50.	Kouta Ouhashi, Satoru Ueno and Saburo Shimabayashi : カチオン性コポリアミノ酸とタンパク質の複合体の膜透過, 第7回長井シンポジウム講演要旨集, Vol.7, P115, Sep. 2006.
51.	Naoko Oda, Satoru Ueno and Saburo Shimabayashi : ヒドロキシアパタイトに対する2種界面活性剤の同時吸着, 第7回長井シンポジウム講演要旨集, Vol.7, P113, Sep. 2006.
52.	Emiko Motoki, Satoru Ueno and Saburo Shimabayashi : ヒドロキシアパタイト表面へのウシ血清アルブミンの吸着に対する界面活性剤添加の影響, 第7回長井シンポジウム講演要旨集, Vol.7, P109, Sep. 2006.
53.	Saburo Shimabayashi, Satoru Ueno, 鍬塚理沙 and 鵜飼大 : 低分子量物質の活性炭に対する吸着に及ぼすPVPとの複合体形成の影響, 第126年会日本薬学会講演要旨集2, 17, Mar. 2006. (Summary) PVPとの複合体形成が吸着量に影響すること，その原因は静電的な反発効果，立体的な障害効果，分子間複合体形成による効果などを考慮する必要のあることなどを指摘した．
54.	Satoru Ueno, 柴田あきら and Saburo Shimabayashi : 負荷電型ポリアミノ酸のリポソーム脂質2分子膜透過, 第126年会日本薬学会講演要旨集2, 21, Mar. 2006. (Summary) リポソーム2分子膜に対するアニオンセイポリアミノ酸の透過性を検討．
55.	井上朋法, Satoru Ueno and Saburo Shimabayashi : 塩酸クロルヘキシジンの溶解度に及ぼす低分子および高分子量物質の添加の影響, 第126年会日本薬学会講演要旨集2, 20, Mar. 2006. (Summary) 塩酸クロルヘキシジンの溶解性について，低分子あるいは高分子を添加して検討した．高分子には溶解性上昇と低下の2つの効果が見られた．
56.	元木恵美子, Satoru Ueno and Saburo Shimabayashi : ヒドロキシアパタイト表面へのウシ血清アルブミン吸着に対する界面活性剤添加の影響, 第126年会日本薬学会講演要旨集2, 17, Mar. 2006. (Summary) BSAのHAPに対する吸着量は界面活性剤のBSAおよびHAPに対する吸着量に依存し，界面活性剤濃度によって，増加と減少が見られた．
57.	Saburo Shimabayashi, Satoru Ueno and 小田奈央子 : ヒドロキシアパタイトに対する2種界面活性剤の同時吸着, 第126年会日本薬学会講演要旨集2, 17, Mar. 2006. (Summary) アルキル燐酸と種々界面活性剤との協同的，競争的吸着を検討した．ヘミミセル，アドミセルの重要性を指摘した．
58.	Satoru Ueno, 柴田あきら and Saburo Shimabayashi : Polyethyleneimine can permeate the lipid bilayer of gigant liposome, 第41回日本界面医学会学術研究会プログラムおよび抄録, 16, Nov. 2005. (Summary) リポソーム膜をカチオン性高分子が透過することを種々の立場から物理化学的に検討した．

Et cetera, Workshop:

1.	Satoru Ueno and Saburo Shimabayashi : Poly(Arg)-nanoparticle complexes translocate lipidbilayer membrane, ABSTRACTS: International Symposium on Nanotoxicology Assessment and Biomedical, Environmemtal Application of Fine Particles and Nanotubes, Vol.1, No.1, Jun. 2008. (Keyword) Arg / HAP / 脂質二分子膜 / 膜透過

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

New strategic study on advanced utilization of rare phosphorus (Project/Area Number: 20241020 )
ナノテクノロジーによる1分子ゲノム解析 (Project/Area Number: 13202048 )
Search by Researcher Number (40176615)

Associate Professor, Associate Professor, Research Associate : Ueno, Satoru

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)