type: misc

label: url

en: ビタミンと生活習慣病(特集:生活習慣病の栄養管理)

ja: ビタミンと生活習慣病(特集:生活習慣病の栄養管理)

en: 成人病と生活習慣病

ja: 成人病と生活習慣病


en: リゾホスファチジン酸による肥満亢進抑制作用

ja: リゾホスファチジン酸による肥満亢進抑制作用

en: Vitamins

ja: ビタミン


en: ビタミンEの抗動脈硬化作用 : CD36の活性化抑制を介した機構

ja: ビタミンEの抗動脈硬化作用 : CD36の活性化抑制を介した機構


en: ビタミンE誘導体のアポトーシス誘導作用 : 構造-活性相関

ja: ビタミンE誘導体のアポトーシス誘導作用 : 構造-活性相関


en: Enhancement of Nitric Oxide and Superoxide Generations by α-Tocopheryl Succinate and Its Apoptotic and Anticancer Effects

en: Tocopheryl succinate (TS), a succinyl ester of alpha-tocopherol (alpha-T), has been reported to have various biological activities. In this communication, we review the current findings about TS including our recent studies of its effects on nitric oxide (NO) and superoxide (O2-) generations implicated in cancer and atherosclerosis. First, we investigated the effect of TS on NO production in vascular smooth muscle cells (VSMC) under atherosclerosis-like conditions using lipopolysaccharide (LPS) and interferon-gamma (IFN). TS enhanced LPS/IFN-dependent NO production, but alpha-T itself did not. The enhancement by TS of NO production was inhibited by alpha-T but not by antioxidants such as ascorbic acid and 2[3]-t-butyl-4-hydroxyanisole (BHA). TS enhanced the amount of protein kinase Calpha (PKCalpha) in VSMC, and PKC inhibitors inhibited TS-enhanced NO production, suggesting that the enhancing effect of TS on NO production is caused by up-regulation of PKC. Second, we found that TS induced apoptosis in VSMC associated with increase in O2- generation via NADPH-dependent oxidase. We further observed that a mouse breast cancer cell line C127I was more susceptible for TS-induced apoptosis than a mouse breast normal cell line NmuMG, and that superoxide dismutase, alpha-T, and BHA inhibited TS-caused morphological cell damage in C127I. From these results, O2- itself and/or other reactive oxygen species are assumed to associate with TS-induced cell toxicity, and antioxidative defense systems are supposed to be lowered in cancer cells. Finally, we found that intravenous injection of TS vesicles completely inhibited the growth of melanoma cells B16-F1 inoculated on the back of hairless mice and enhanced their survival time.

en: Biochemistry (Moscow)

ja: Biochemistry (Moscow)


en: Tocopheryl succinate-versatile function due to its unique physicochemical properties

en: Journal of Clinical Biochemistry and Nutrition


en: ビタミンEコハク酸の血管新生阻害作用 : 新規抗がん剤としての可能性

ja: ビタミンEコハク酸の血管新生阻害作用 : 新規抗がん剤としての可能性


en: 喫煙が血液中で悪玉酸化リン脂質をつくる

ja: 喫煙が血液中で悪玉酸化リン脂質をつくる


en: Generation of platelet-activating factor (PAF)-like phospholipids in blood circulation under elevated oxidative stress:its pathological significance on atherosclerosis

ja: Journal of Clinical Biochemistry and Nutrition


en: ビタミンEコハク酸 : アポトーシス誘導作用と抗ガン剤としての可能性

ja: ビタミンEコハク酸 : アポトーシス誘導作用と抗ガン剤としての可能性


en: 喫煙が血液中で悪玉リン脂質をつくるー動脈硬化との関連ー

ja: 喫煙が血液中で悪玉リン脂質をつくるー動脈硬化との関連ー

en: 日本薬学会 第122年会講演ハイライト

ja: 日本薬学会 第122年会講演ハイライト


en: 血管障害の抑制などγ-トコフェロールに新たに見いだされた作用

ja: 血管障害の抑制などγ-トコフェロールに新たに見いだされた作用


en: Kinetics and Dynamics of α-Tocopherol and Carotenoids for Deactivating Singlet Oxygen in Phospholipid Membranes

ja: α-トコフェロールおよびカロテノイドのリン脂質膜における存在様態と一重項酸素不活化反応のキネティクス

en: The singlet oxygen (^1O_2) deactivating rate constants (k_s) of α-tocopherol (α-Toc) and carotenoids such as β-carotene, astaxanthin and canthaxanthin and their inhibiting activities for ^1O_2-dependentlipid peroxidation (IC_50' that is, the concentration required for 50% inhibition of lipid peroxidation) were investigated in liposomes and compared to those in EtOH solution. Liposomes were prepared from dimyristoylphosphatidylcholine to measure the k_s and egg yolk phosphatidylcholine to measure the IC_50 values. ^1O_2 was generated by photoirradiation using three photosensitizers, Rose bengal and methylene blue, which generate ^1O_2 at the membrane surface or in bulk water, and 2-(1-pyrene) dodecanoic acid, which generates ^1O_2 in the membrane hydrophobic inner region. The k_s and IC_50 values were determined in membranes considering the following two points: (1) antioxidants exist in membranes and their active moieties are distributed in different regions of membranes at different concentrations; (2) dynamics of ^1O_2 in membranes (generation site of ^1O_2 depending on the localization of photosensitizer in membranes, and solubility of ^1O_2 which differs in the site of membranes). In conclusion, the following three factors are experimentally confirmed to be important for the consideration of ^1O_2 deactivating activities of α-Toc and carotenoids in membranes:(1) the concentration of antioxidants (EtOH solution < membrane), especially the local concentration of their active moieties in membranes (in the case of OH-group of α-Toc, 0%, 50-60%, and 40-50% at polar zone, hydrogen belt, and hydrophobic core, respectively, of the membranes); (2) the mobility of antioxidants (EtOH solution > membrane), the mobility of membrane phospholipid (liquid crystalline state > gel state), the mobility of antioxidants in membranes (antioxidants located at one half of the bilayer membrane such as α-tocopherol > antioxidants located across the bilayer such as β-carotene; antioxidants interactive with polar head group of lipid in membrane such as astaxanthin < antioxidants nointeractive with polar head group of lipid in membrane such as β-carotene), and the local mobility of their active moieties (in case of OH-group of α-Toc, membrane surface < membrane inner region); (3) the dielectric constant (micropolarity) at the domains in membranes, that is higher in membrane surface than in membrane inner region, where the active moieties of antioxidants deactivate ^1O_2 (in case of α-Toc, reactivity is high in the solution with high dielectric constant).


en: リン脂質過酸化の二次生成物が特異的受容体に作用して動脈硬化の進展に寄与する

ja: リン脂質過酸化の二次生成物が特異的受容体に作用して動脈硬化の進展に寄与する


en: Platelet-activating factor (PAF)- like oxidized phospholipids: relevance to atherosclerosis

en: Lipid peroxidation is involved in the pathogenesis of chronic diseases including atherosclerosis. Oxidized lipoprotein has diverse biological activities and is believed to initiate atheroma formation and maturate fatty plaque. The active components of oxidized lipoproteins still remain to be clarified, but a likely candidate is the phosphatidylcholine (PC) having an sn-2-short-chain acyl group with a methyl, hydroxyl, aldehydic or carboxylic terminal. These unique PCs, formed by oxidative fragmentation of the polyunsaturated acyl group of the parent PC in liposomes, low density lipoproteins and blood plasma, induce platelet aggregation through the activation of the receptor for platelet-activating factor (PAF), due to their resemblance in structure with PAF. We have found that PAF-like lipids regulate DNA synthesis and production of nitric oxide independently of the activation of the PAF receptor in vascular smooth muscle cells. Regulation of vascular cell function through two distinct signaling pathways mediated by PAF-like lipids provides new insight into the mechanism of induction of atherosclerosis.

en: BioFactors

ja: BioFactors


en: リポソーム膜においてアスコルビン酸-Fe2+により誘導される脂質過酸化反応に対するトコフェロールの抗酸化作用