read0187881/published_papers/44254812 Mahadi Hasan Anowara Khatun Kentaro Kogure Intradermal Delivery of Naked mRNA Vaccines via Iontophoresis Messenger RNA (mRNA) vaccines against infectious diseases and for anticancer immunotherapy have garnered considerable attention. Currently, mRNA vaccines encapsulated in lipid nanoparticles are administrated via intramuscular injection using a needle. However, such administration is associated with pain, needle phobia, and lack of patient compliance. Furthermore, side effects such as fever and anaphylaxis associated with the lipid nanoparticle components are also serious problems. Therefore, noninvasive, painless administration of mRNA vaccines that do not contain other problematic components is highly desirable. Antigen-presenting cells reside in the epidermis and dermis, making the skin an attractive vaccination site. Iontophoresis (ItP) uses weak electric current applied to the skin surface and offers a noninvasive permeation technology that enables intradermal delivery of hydrophilic and ionic substances. ItP-mediated intradermal delivery of biological macromolecules has also been studied. Herein, we review the literature on the use of ItP technology for intradermal delivery of naked mRNA vaccines which is expected to overcome the challenges associated with mRNA vaccination. In addition to the physical mechanism, we discuss novel biological mechanisms of iontophoresis, particularly ItP-mediated opening of the skin barriers and the intracellular uptake pathway, and how the combined mechanisms can allow for effective intradermal delivery of mRNA vaccines. MDPI AG Pharmaceutics 1999-4923 15 12 2678 2678 20231126 10.3390/pharmaceutics15122678 read0187881/published_papers/39640347 Misaki Yamasaki Yuika Seto Mizune Ozono Michiyasu Nakao Akira Shigenaga Akira Otaka Shigeki Sano Kentaro Kogure Development of a novel tocopheryl ester for suppression of lipid accumulation without cytotoxicity by optimization of dicarboxylic ester moiety Elsevier BV Biochemistry and Biophysics Reports 2405-5808 31 101329 101329 20220900 10.1016/j.bbrep.2022.101329 read0187881/published_papers/37828341 Mahadi Hasan Tatsuya Fukuta Shinya Inoue Hinako Mori Mayuko Kagawa Kentaro Kogure Iontophoresis-mediated direct delivery of nucleic acid therapeutics, without use of carriers, to internal organs via non-blood circulatory pathways Elsevier BV Journal of Controlled Release 0168-3659 343 392 399 20220300 10.1016/j.jconrel.2022.01.052 read0187881/published_papers/37828343 Mahadi Hasan Anowara Khatun Kentaro Kogure Iontophoresis of Biological Macromolecular Drugs Over the last few decades, biological macromolecular drugs (e.g., peptides, proteins, and nucleic acids) have become a significant therapeutic modality for the treatment of various diseases. These drugs are considered superior to small-molecule drugs because of their high specificity and favorable safety profiles. However, such drugs are limited by their low oral bioavailability and short half-lives. Biological macromolecular drugs are typically administrated via invasive methods, e.g., intravenous or subcutaneous injections, which can be painful and induce needle phobia. Noninvasive transdermal delivery is an alternative administration route for the local and systemic delivery of biological macromolecular drugs. However, a challenge with the noninvasive transdermal delivery of biological macromolecular drugs is the outermost layer of the skin, known as the stratum corneum, which is a physical barrier that restricts the entry of extraneous macromolecules. Iontophoresis (IP) relies on the application of a low level of electricity for transdermal drug delivery, in order to facilitate the skin permeation of hydrophilic and charged molecules. The IP of several biological macromolecular drugs has recently been investigated. Herein, we review the IP-mediated noninvasive transdermal delivery of biological macromolecular drugs, their routes of skin permeation, their underlying mechanisms, and their advance applications. MDPI AG Pharmaceutics 1999-4923 14 3 525 525 20220226 10.3390/pharmaceutics14030525 read0187881/published_papers/37828344 Anowara Khatun Mahadi Hasan Mahran Mohamed Abd El-Emam Tatsuya Fukuta Miyuki Mimura Riho Tashima Shintaro Yoneda Shintaro Yoshimi Kentaro Kogure Effective Anticancer Therapy by Combination of Nanoparticles Encapsulating Chemotherapeutic Agents and Weak Electric Current Pharmaceutical Society of Japan Biological and Pharmaceutical Bulletin 1347-5215 45 2 194 199 20220201 10.1248/bpb.b21-00714 read0187881/published_papers/29520487 Rumana Yesmin Hasi Dai Majima Katsuya Morito Hanif Ali Kentaro Kogure Meera Nanjundan Junji Hayashi Ryushi Kawakami Kaori Kanemaru Tamotsu Tanaka Isolation of glycosylinositol phosphoceramide and phytoceramide 1-phosphate in plants and their chemical stabilities. Glycosylinositol phosphoceramide (GIPC) is a sphingophospholipid in plants. Recently, we identified that GIPC is hydrolyzed to phytoceramide 1-phosphate (PC1P) by an uncharacterized phospholipase D activity following homogenization of certain plant tissues. We now developed methods for isolation of GIPC and PC1P from plant tissues and characterized their chemical stabilities. Hydrophilic solvents, namely a lower layer of a mixed solvent system consisting of isopropanol/hexane/water (55:20:25, v/v/v) was efficient solvent for extraction and eluent in column chromatography. GIPC was isolated by Sephadex column chromatography followed by TLC. A conventional method, such as the Bligh and Dyer method, was applicable for PC1P extraction. Specifically, PC1P was isolated by TLC following mild alkali treatment of lipid extracts of plants. The yields of GIPC and PC1P in our methods were both around 50-70%. We found that PC1P is tolerant against heat (up to 125 °C), strong acid (up to 10 M HCl), and mild alkali (0.1 M KOH). In contrast, significant degradation of GIPC occurred at 100 °C and 1.0 M HCl treatment, suggesting the instability of the inositol glycan moiety in these conditions. These data will be useful for further biochemical and nutritional studies on these sphingolipids. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 1152 122213 122213 20200612 10.1016/j.jchromb.2020.122213 32615533 read0187881/published_papers/26743746 Tasuku Torao Miyuki Mimura Yasufumi Oshima Koki Fujikawa Mahadi Hasan Tatsuharu Shimokawa Naoshi Yamazaki Hidenori Ando Tatsuhiro Ishida Tatsuya Fukuta Tamotsu Tanaka Kentaro Kogure Characteristics of unique endocytosis induced by weak current for cytoplasmic drug delivery. We previously reported that a weak current (WC, 0.3-0.5 mA/cm2) applied to cells can induce endocytosis to promote cytoplasmic delivery of hydrophilic macromolecules (MW: <70,000), such as dextran and siRNA, which leak from WC-induced endosomes into the cytoplasm (Hasan et al., 2016). In this study, we evaluated the characteristics of WC-mediated endocytosis for application of the technology to cytoplasmic delivery of macromolecular medicines. WC induced significantly higher cellular uptake of exogenous DNA fragments compared to untreated cells; the amount increased in a time-dependent manner, indicating that endocytosis was induced after WC. Moreover, following WC treatment of cells in the presence of an antibody (MW: 150,000) with the lysosomotropic agent chloroquine, the antibody was able to bind to its intracellular target. Thus, high molecular weight protein medicines delivered by WC-mediated endocytosis were functional in the cytoplasm. Transmission electron microscopy of cells treated by WC in the presence of gold nanoparticles covered with polyethylene glycol showed that the WC-induced endosomes exhibited an elliptical shape. In the WC-induced endosomes, ceramide, which makes pore structures in the membrane, was localized. Together, these results suggest that WC can induce unique endocytosis and that macromolecular medicines leak from endosomes through a ceramide pore. International journal of pharmaceutics 576 119010 119010 20200225 10.1016/j.ijpharm.2019.119010 31901359 read0187881/published_papers/13409553 Tatsuya Fukuta Shota Hirai Tatsusada Yoshida Takashi Maoka Kentaro Kogure Enhancement of antioxidative activity of astaxanthin by combination with an antioxidant capable of forming intermolecular interactions. We previously demonstrated that coencapsulation of the potent antioxidant astaxanthin (Asx) and tocotrienols into liposomes results in synergistically higher antioxidative activity than the calculated additive activity of each individual antioxidant-containing liposome, due to intermolecular interactions between terminal ring moieties of the two antioxidants and the polyene chain and the triene moiety. We reported that intermolecular interactions depend on the stereochemistry of Asx, and change the electronic state of the Asx polyene moiety. Based on these findings, we hypothesized that antioxidants that interact with Asx at the terminal ring and polyene moieties may enhance the antioxidative activity. Herein, we selected two candidate antioxidants, capsaicin (Cap) and resveratrol, based on their structures, in which the compounds exhibit similar characteristics to tocotrienols. We evaluated the antioxidative capacities of liposomes coencapsulating Asx and the selected candidates. Based on hydroxyl radical scavenging activity, Cap was found to synergistically enhance the antioxidative activity of Asx at an optimal Asx/Cap ratio. Intermolecular interactions between Asx and Cap are necessary for the synergistic effect, and the Asx stereoisomer 3R,3'R-form (Asx-R) was predicted to most potently interact. Liposomes coencapsulating Asx-R and Cap exhibited clear synergistic antioxidative activity at an optimal ratio, whereas liposomes coencapsulating the other Asx stereoisomer and Cap did not demonstrate such activity. Computational chemistry analysis showed that changes in the electronic state of the polyene moiety of Asx-R are crucial for the synergistic activity. These results suggest that antioxidants that can change the electronic state of Asx via intermolecular interactions may enhance the function of Asx. Free radical research 1071-5762 1 231 20191113 10.1080/10715762.2019.1693042 31718328 read0187881/published_papers/13409554 S Hama Y Okamura K Kamei S Nagao M Hayashi S Maeda Kenji Fukuzawa Kentaro Kogure α-Tocopheryl succinate stabilizes the structure of tumor vessels by inhibiting angiopoietin-2 expression α-Tocopheryl succinate (TS) is a tocopherol derivative and has multifaceted anti-cancer effects; TS not only causes cancer cell-specific apoptosis but also inhibits tumor angiogenesis. Although TS has the potential to be used as a well-tolerated anti-angiogenic drug, it is still unclear which step of the angiogenic process is inhibited by TS. Here, we show that TS inhibits the expression of angiopoietin (Ang)-2, which induces destabilization of vascular structure in the initial steps of the angiogenic process. In mouse melanoma cells, TS treatment decreased mRNA and extracellular protein levels of Ang-2; however, the mRNA level of Ang-1, which stabilizes the vascular structure, remained unchanged. Furthermore, aorta ring and Matrigel plug angiogenesis assays indicated that the conditioned medium from TS-treated cells (CM-TS) inhibited neovascularization and blood leakage from the existing blood vessels, respectively. Following immunohistochemical staining of the vessels treated with CM-TS, imaging studies showed that the vascular endothelial cells were highly packed with pericytes. In conclusion, we found that TS inhibits Ang-2 expression and, consequently, stabilizes the vascular structure during the initial step of tumor angiogenesis. Biochemical and Biophysical Research Communications 0006-291X null null 20191109 10.1016/j.bbrc.2019.11.017 31718795 read0187881/published_papers/402403 Rumana Yesmin Hasi Makoto Miyagi Katsuya Morito Toshiki Ishikawa Maki Kawai-Yamada Hiroyuki Imai Tatsuya Fukuta Kentaro Kogure Kaori Kanemaru Junji Hayashi Ryushi Kawakami Tamotsu Tanaka Glycosylinositol phosphoceramide-specific phospholipase D activity catalyzes transphosphatidylation. Glycosylinositol phosphoceramide (GIPC) is the most abundant sphingolipid in plants and fungi. Recently, we detected GIPC-specific phospholipase D (GIPC-PLD) activity in plants. Here, we found that GIPC-PLD activity in young cabbage leaves catalyzes transphosphatidylation. The available alcohol for this reaction is a primary alcohol with a chain length below C4. Neither secondary alcohol, tertiary alcohol, choline, serine nor glycerol serves as an acceptor for transphosphatidylation of GIPC-PLD. We also found that cabbage GIPC-PLD prefers GIPC containing two sugars. Neither inositol phosphoceramide, mannosylinositol phosphoceramide nor GIPC with three sugar chains served as substrate. GIPC-PLD will become a useful catalyst for modification of polar head group of sphingophospholipid. Journal of biochemistry 0021-924X 166 5 441 448 20191101 10.1093/jb/mvz056 31504617 read0187881/published_papers/402402 Katsuya Morito Ryota Shimizu Nahoko Kitamura N Kitamura Si-Bum Park B S Park Shigenobu Kishino S Kishino Jun Ogawa J Ogawa Tatsuya Fukuta Kentaro Kogure Tamotsu Tanaka Gut microbial metabolites of linoleic acid are metabolized by accelerated peroxisomal β-oxidation in mammalian cells. Gut microbial metabolites of linoleic acid are metabolized by accelerated peroxisomal β-oxidation in mammalian cells Microorganisms in animal gut produce unusual fatty acids from the ingested diet. Two types of hydroxy fatty acids (HFAs), 10-hydroxy-cis-12-octadecenoic acid (HYA) and 10-hydroxy-octadecanoic acid (HYB), are linoleic acid (LA) metabolites produced by Lactobacillus plantarum. In this study, we investigated the metabolism of these HFAs in mammalian cells. When Chinese hamster ovary (CHO) cells were cultured with HYA, approximately 50% of the supplemented HYA disappeared from the dish within 24 h. On the other hand, the amount of HYA that disappeared from the dish of peroxisome (PEX)-deficient CHO cells was lower than 20%. Significant amounts of C2- and C4-chain-shortened metabolites of HYA were detected in culture medium of HYA-supplemented CHO cells, but not in medium of PEX-deficient cells. These results suggested that peroxisomal β-oxidation is involved in the disappearance of HYA. The PEX-dependent disappearance was observed in the experiment with HYB, but not with LA. We also found that HYA treatment up-regulates peroxisomal β-oxidation activity of human gastric MKN74 cells and intestinal Caco-2 cells. These results indicate a possibility that HFAs produced from gut bacteria affect lipid metabolism of host via modulation of peroxisomal β-oxidation activity. Microorganisms in animal gut produce unusual fatty acids from the ingested diet. Two types of hydroxy fatty acids (HFAs), 10-hydroxy-cis-12-octadecenoic acid (HYA) and 10-hydroxy-octadecanoic acid (HYB), are linoleic acid (LA) metabolites produced by Lactobacillus plantarum. In this study, we investigated the metabolism of these HFAs in mammalian cells. When Chinese hamster ovary (CHO) cells were cultured with HYA, approximately 50% of the supplemented HYA disappeared from the dish within 24 h. On the other hand, the amount of HYA that disappeared from the dish of peroxisome (PEX)-deficient CHO cells was lower than 20%. Significant amounts of C2- and C4-chain-shortened metabolites of HYA were detected in culture medium of HYA-supplemented CHO cells, but not in medium of PEX-deficient cells. These results suggested that peroxisomal β-oxidation is involved in the disappearance of HYA. The PEX-dependent disappearance was observed in the experiment with HYB, but not with LA. We also found that HYA treatment up-regulates peroxisomal β-oxidation activity of human gastric MKN74 cells and intestinal Caco-2 cells. These results indicate a possibility that HFAs produced from gut bacteria affect lipid metabolism of host via modulation of peroxisomal β-oxidation activity. Biochimica et biophysica acta. Molecular and cell biology of lipids Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1388-1981 1864 11 1619 1628 20191100 10.1016/j.bbalip.2019.07.010 31351225 read0187881/published_papers/25126720 Suzuki T Wakao Y Watanabe T Hori M Ikeda Y Tsuchiya H Kogure K Harada-Shiba M Fujimuro M Kamiya H No enhancing effects of plasmid-specific histone acetyltransferase recruitment system on transgene expression in vivo. Nucleosides, nucleotides & nucleic acids 1532-2335 38 12 1 8 20190700 10.1080/15257770.2019.1638514 31299884 2-s2.0-85068907161 read0187881/published_papers/34202642 紙谷 浩之 鈴木 哲矢 若尾 祐介 渡部 匡史 堀 美香 池田 義人 土谷 博之 小暮 健太朗 斯波 真理子 藤室 雅弘 プラスミド特異的ヒストンアセチル化酵素リクルートシステムの効果はin vivoでは観察されない 日本DDS学会 日本DDS学会学術集会プログラム予稿集 35 182 182 20190600 read0187881/published_papers/402405 Tatsuya Fukuta Shintaroh Yoshimi Tamotsu Tanaka Kentaro Kogure Leukocyte-mimetic liposomes possessing leukocyte membrane proteins pass through inflamed endothelial cell layer by regulating intercellular junctions Nanoparticles such as liposomes have been applied for the treatment of various diseases such as cancer and inflammatory diseases by utilizing the enhanced permeability and retention effect. However, their entry into inflammation sites is still limited since passive delivery of nanoparticles is often hampered by the presence of endothelial barriers. As leukocytes can pass through the inflamed endothelium via utilizing membrane protein functions, we hypothesized that incorporating leukocyte membrane proteins onto liposomal membranes may impart leukocyte-mimicking functions to liposomes, allowing for their adherence to and active passage through the inflamed endothelium. Herein, we developed leukocyte-mimetic liposomes (LM-Lipo) by leukocyte membrane protein transfer and evaluated their function in vitro. Transfer of membrane proteins from human leukemia cells onto liposomal membranes allowed for significant association of the liposomes with inflamed human endothelial cells, and subsequent passage through inflamed endothelial cell layer. The confocal images showed that LM-Lipo significantly induced vascular endothelial-cadherin displacement. These results indicate that LM-Lipo adhered to and regulated intercellular junctions of inflamed endothelial cell layer, resulting in passage through the layer, by mimicking the function of leukocytes. Furthermore, it is suggested that liposomes possessing leukocyte-like functions could be useful for drug delivery to inflammation sites by overcoming endothelial barriers. International Journal of Pharmaceutics 0378-5173 563 314 323 20190500 10.1016/j.ijpharm.2019.04.027 30978483 read0187881/published_papers/13409555 Mahadi Hasan Susumu Hama Kentaro Kogure Low electric treatment activates Rho GTPase via heat shock protein 90 and protein kinase c for intracellular delivery of siRNA Springer Science and Business Media LLC Scientific Reports 2045-2322 9 1 4114 4114 20190300 10.1038/s41598-019-40904-z 30858501 read0187881/published_papers/402404 Tetsuya Suzuki Yusuke Wakao Tadashi Watanabe Mika Hori Yoshito Ikeda Hiroyuki Tsuchiya Kentaro Kogure Mariko Harada-Shiba Masahiro Fujimuro Hiroyuki Kamiya No enhancing effects of plasmid-specific histone acetyltransferase recruitment system on transgene expression in vivo. Altered levels of histone acetylation are associated with changes in chromosomal gene expression. Thus, the specific acetylation of histones bound to plasmid DNA might increase transgene expression. Previously, the expression of the histone acetyltransferase domain of CREB-binding protein fused to the sequence-dependent DNA binding domain of GAL4 (GAL4-HAT) successfully improved reporter gene expression in cultured cells [J. Biosci. Bioengng. 123, 277-280 (2017)]. In this study, the same approach was applied for transgene expression in mice. The activator and reporter plasmid DNAs bearing the genes for GAL4-HAT and Gaussia princeps luciferase, respectively, were co-administered into the mouse liver by hydrodynamics-based tail vein injection, and the Gaussia luciferase activity in serum was measured for two weeks. Unexpectedly, the co-injection of the GAL4-HAT and luciferase plasmid DNAs seemed to decrease, rather than increase, luciferase expression. Moreover, the co-injection apparently reduced the amount of luciferase DNA in the liver. These results indicated that this system is ineffective in vivo and suggested the exclusion of hepatic cells expressing GAL4-HAT. Nucleosides, nucleotides & nucleic acids 1532-2335 38 12 942 949 20190000 10.1080/15257770.2019.1638514 31299884 read0187881/published_papers/34114366 Dai Majima Ryosuke Mitsuhashi Tatsuya Fukuta Tamotsu Tanaka Kentaro Kogure Biological Functions of α-Tocopheryl Succinate. α-Tocopheryl succinate (TS) is a succinic acid ester of a well-known natural antioxidant α-tocopherol (α-T). Physicochemical characteristics of TS are entirely different from the original compound α-T. TS becomes vesicles via forming a lamella structure. Furthermore, although the antioxidative activity of α-T is lacked by esterification of phenolic hydroxyl (OH) moiety with succinate, TS has versatile biological functions, such as inhibition of cholinesterase activity, inhibition of nuclear factor-kappa B (NF-κB) activation, enhancement of lipopolysaccharide-induced nitric oxide production, and anticancer effect. Especially, we expect TS as a novel anticancer agent. TS nanovesicle shows significant anticancer activity in vitro and in vivo. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase produces superoxide which mediates the anticancer activity of TS. Moreover, it suggests that TS activates protein kinase C via direct interaction. Based on the analysis of structure and activity relationship, it ensures that succinate moiety of TS plays a vital role in anticancer activity. This review introduces the detail and mechanism of versatile biological functions of TS. Journal of nutritional science and vitaminology 65 Supplement S104-S108 null 20190000 10.3177/jnsv.65.S104 31619606 read0187881/published_papers/25696788 Tetsuya Suzuki Yusuke Wakao Tadashi Watanabe Mika Hori Yoshito Ikeda Hiroyuki Tsuchiya Kentaro Kogure Mariko Harada-Shiba Masahiro Fujimuro Hiroyuki Kamiya Effects of plasmid-specific histone acetyltransferase recruitment system in vivo. Nucleosides Nucleotides Nucleic Acids 38 12 942 949 20190000 read0187881/published_papers/15293349 Tatsuharu Shimokawa Tatsuya Fukuta T Inagi Kentaro Kogure Protective effect of high-affinity liposomes encapsulating astaxanthin against corneal disorder in the in vivo rat dry eye disease model Journal of Clinical Biochemistry and Nutrition 0912-0009 null null 20190000 read0187881/published_papers/13409556 Tatsuya Fukuta Akina Nishikawa Kentaro Kogure Low level electricity increases the secretion of extracellular vesicles from cultured cells Biochemistry and Biophysics Reports 2405-5808 null null 20190000 read0187881/published_papers/28399724 立花 洸季 西辻 和親 田中 保 小暮 健太朗 石田 竜弘 奥平 桂一郎 スフィンゴシン-1-リン酸(S1P)による高密度リポプロテイン(HDL)構成タンパク質分泌への影響 (公社)日本生化学会 日本生化学会大会プログラム・講演要旨集 91 [1P 058] 20180900 read0187881/published_papers/26743499 Misuzu Ishikawa Shota Hirai Tatsusada Yoshida Natsumi Shibuya Susumu Hama Yu Takahashi Tatsuya Fukuta Tamotsu Tanaka Shinzo Hosoi Kentaro Kogure Carotenoid Stereochemistry Affects Antioxidative Activity of Liposomes Co-encapsulating Astaxanthin and Tocotrienol We previously found that antioxidative activity of liposomes co-encapsulating astaxanthin (Asx) and tocotrienols (T3s) was higher than the calculated additive activity, which results from intermolecular interactions between both antioxidants (J. Clin. Biochem. Nutr., 59, 2016, Kamezaki et al.). Herein, we conducted experiments to optimize Asx/α-T3 ratio for high antioxidative activity, and tried to elucidate details of intermolecular interaction of Asx with α-T3. Higher activity than calculated additive value was clearly observed at an Asx/α-T3 ratio of 2 : 1, despite two α-T3 would potentially interact with two terminal rings of one Asx. The synthetic Asx used in this study was a mixture of three stereoisomers, 3R,3&#039;R-form (Asx-R), 3S,3&#039;S-form (Asx-S) and 3R,3&#039;S-meso form (Asx-meso). The calculated binding energy of the Asx-S/α-T3 complex was higher than those of Asx-R/α-T3 and Asx-meso/α-T3, suggesting that Asx-S and α-T3 is the most preferable combination for the intermolecular interaction. The optimal Asx-S/α-T3 ratio for antioxidation was shown to be 1 : 2. These results suggest that the Asx stereochemistry affects the intermolecular interaction of Asx/α-T3. Moreover, the absorption spectrum changes of Asx-S upon co-encapsulation with α-T3 in liposomes indicate that the electronic state of Asx-S is affected by intermolecular interactions with α-T3. Further, intermolecular interactions with α-T3 affected the electronic charges on the C9, C10 and C15 atoms in the polyene moiety of Asx-S. In conclusion, the intermolecular interaction of Asx/T3 depends on the Asx stereochemistry, and caused a change in the electronic state of the Asx polyene moiety by the presence of double bond in the T3 triene moiety. Chemical & Pharmaceutical Bulletin 1347-5223 66 7 714 720 20180500 10.1248/cpb.c18-00035 29962454 read0187881/published_papers/1797001 Ishikawa M 小暮 健太朗 Hirai S Yoshida T Shibuya N Hama S Takahashi Y Fukuta T Tanaka T Hosoi S Kogure K Carotenoid stereochemistry affects antioxidative activity of liposomes co-encapsulating astaxanthin and tocotrienol. We previously found that antioxidative activity of liposomes co-encapsulating astaxanthin (Asx) and tocotrienols (T3s) was higher than the calculated additive activity, which results from intermolecular interactions between both antioxidants (J. Clin. Biochem. Nutr., 59, 2016, Kamezaki et al.). Herein, we conducted experiments to optimize Asx/α-T3 ratio for high antioxidative activity, and tried to elucidate details of intermolecular interaction of Asx with α-T3. Higher activity than calculated additive value was clearly observed at an Asx/α-T3 ratio of 2 : 1, despite two α-T3 would potentially interact with two terminal rings of one Asx. The synthetic Asx used in this study was a mixture of three stereoisomers, 3R,3&#039;R-form (Asx-R), 3S,3&#039;S-form (Asx-S) and 3R,3&#039;S-meso form (Asx-meso). The calculated binding energy of the Asx-S/α-T3 complex was higher than those of Asx-R/α-T3 and Asx-meso/α-T3, suggesting that Asx-S and α-T3 is the most preferable combination for the intermolecular interaction. The optimal Asx-S/α-T3 ratio for antioxidation was shown to be 1 : 2. These results suggest that the Asx stereochemistry affects the intermolecular interaction of Asx/α-T3. Moreover, the absorption spectrum changes of Asx-S upon co-encapsulation with α-T3 in liposomes indicate that the electronic state of Asx-S is affected by intermolecular interactions with α-T3. Further, intermolecular interactions with α-T3 affected the electronic charges on the C9, C10 and C15 atoms in the polyene moiety of Asx-S. In conclusion, the intermolecular interaction of Asx/T3 depends on the Asx stereochemistry, and caused a change in the electronic state of the Asx polyene moiety by the presence of double bond in the T3 triene moiety. Chem. Pharm. Bull. 0009-2363 66 7 714 720 20180500 10.1248/cpb.c18-00035 29962454 read0187881/published_papers/13781074 Shimokawa T 小暮 健太朗 Yoshida M Fukuta T Tanaka T Inagi T Kogure K Efficacy of high-affinity liposomal astaxanthin on up-regulation of age-related markers induced by oxidative stress in human corneal epithelial cells Decreases in tear volume, unstable tear films and excessive tear evaporation are known to cause desiccation and hyperosmolar stress. These, in turn, induce oxidative stress that is thought to cause dry eye, which is also considered to be age-related disease. We hypothesized that oxidative stress induces up-regulation of age-related markers, and that the antioxidant astaxanthin prepared as a liposomal formulation may be a candidate for the treatment of dry eye. Herein, we examined age-related markers in an dry eye model, and evaluated the efficacy of high-affinity liposomes containing astaxanthin. The dry eye model showed desiccation time-dependent increases in reactive oxygen species. We confirmed the up-regulation of p53, p21 and p16 as a function of desiccation time. Pretreatment with both neutral and slightly-positively-charged astaxanthin liposomal formulations showed significant suppression of up-regulation of all markers, with the positively-charged liposomes exhibiting the greatest efficacy. Furthermore, positively-charged liposomes labeled with fluorescent dyes demonstrated much higher affinity to normal human corneal epithelial cells (HCECs) than neutral liposomes. Taken together, we confirmed the up-regulation of age-related markers, especially p16, in an dry eye model, and demonstrated the potential of high-affinity liposomal astaxanthin for the treatment of dry eye. J. Clin. Biochem. Nutr 0912-0009 64 1 27 35 20180400 10.3164/jcbn.18-27 30705509 read0187881/published_papers/13781126 Sheuli Afroz Ayano Yagi Kouki Fujikawa M Motiur Rahman Katsuya Morito Tatsuya Fukuta Shiro Watanabe Emi Kiyokage Kazunori Toida Taro Shimizu Tatsuhiro Ishida Kentaro Kogure Akira Tokumura Tamotsu Tanaka Lysophosphatidic acid in medicinal herbs enhances prostaglandin E2 and protects against indomethacin-induced gastric cell damage in vivo and in vitro. Lysophosphatidic acid (LPA) is a bioactive phospholipid that induces diverse biological responses. Recently, we found that LPA ameliorates NSAIDs-induced gastric ulcer in mice. Here, we quantified LPA in 21 medicinal herbs used for treatment of gastrointestinal (GI) disorders. We found that half of them contained LPA at relatively high levels (40-240 μg/g) compared to soybean seed powder (4.6 μg/g), which we previously identified as an LPA-rich food. The LPA in peony (Paeonia lactiflora) root powder is highly concentrated in the lipid fraction that ameliorates indomethacin-induced gastric ulcer in mice. Synthetic 18:1 LPA, peony root LPA and peony root lipid enhanced prostaglandin E2 production in a gastric cancer cell line, MKN74 cells that express LPA2 abundantly. These materials also prevented indomethacin-induced cell death and stimulated the proliferation of MKN74 cells. We found that LPA was present in stomach fluids at 2.4 μM, which is an effective LPA concentration for inducing a cellular response in vitro. These results indicated that LPA is one of the active components of medicinal herbs for the treatment of GI disorder and that orally administered LPA-rich herbs may augment the protective actions of endogenous LPA on gastric mucosa. Prostaglandins & other lipid mediators 1098-8823 135 36 44 20180300 10.1016/j.prostaglandins.2018.01.003 29462674 read0187881/published_papers/13781154 Satoko Suzuki Shoko Itakura Ryo Matsui Kayoko Nakayama Takayuki Nishi Akinori Nishimoto Susumu Hama Kentaro Kogure Tumor Microenvironment-Sensitive Liposomes Penetrate Tumor Tissue via Attenuated Interaction of the Extracellular Matrix and Tumor Cells and Accompanying Actin Depolymerization American Chemical Society Biomacromolecules 1526-4602 18 2 535 543 20170213 10.1021/acs.biomac.6b01688 28055201 2-s2.0-85012306090 read0187881/published_papers/28027650 Takashi Kida Aoi Itoh Akari Kimura Hisatsugu Matsuoka Hiroyuki Imai Kentaro Kogure Akira Tokumura Tamotsu Tanaka Distribution of glycosylinositol phosphoceramide-specific phospholipase D activity in plants JOURNAL OF BIOCHEMISTRY 1756-2651 161 2 187 195 20170200 10.1093/jb/mvw060 read0187881/published_papers/25339498 Satoko Suzuki Shoko Itakura Ryo Matsui Kayoko Nakayama Takayuki Nishi Akinori Nishimoto Susumu Hama Kentaro Kogure Tumor Microenvironment-Sensitive Liposomes Penetrate Tumor Tissue via Attenuated Interaction of the Extracellular Matrix and Tumor Cells and Accompanying Actin Depolymerization BIOMACROMOLECULES 1526-4602 18 2 535 543 20170200 10.1021/acs.biomac.6b01688 28055201 2-s2.0-85012306090 read0187881/published_papers/13781170 Takashi Kida Aoi Itoh Akari Kimura Hisatsugu Matsuoka Hiroyuki Imai Kentaro Kogure Akira Tokumura Tamotsu Tanaka Distribution of glycosylinositol phosphoceramide-specific phospholipase D activity in plants Oxford University Press Journal of Biochemistry 1756-2651 161 2 187 195 20170201 10.1093/jb/mvw060 28175321 2-s2.0-85015919191 read0187881/published_papers/13781189 Hyunkyung Jung Yuri Shimatani Mahadi Hasan Kohei Uno Susumu Hama Kentaro Kogure Development of flexible nanocarriers for siRNA delivery into tumor tissue Elsevier B.V. International Journal of Pharmaceutics 1873-3476 516 1-2 258 265 20170110 10.1016/j.ijpharm.2016.11.042 27871835 2-s2.0-84997108932 read0187881/published_papers/25339499 Hyunkyung Jung Yuri Shimatani Mahadi Hasan Kohei Uno Susumu Hama Kentaro Kogure Development of flexible nanocarriers for siRNA delivery into tumor tissue INTERNATIONAL JOURNAL OF PHARMACEUTICS 1873-3476 516 1-2 258 265 20170100 10.1016/j.ijpharm.2016.11.042 27871835 2-s2.0-84997108932 read0187881/published_papers/1797040 Kanako Shiota Susumu Hama Toru Yoshitomi Yukio Nagasaki Kentaro Kogure Prevention of UV-induced melanin production by accumulation of redox nanoparticles in the epidermal layer via iontophoresis Pharmaceutical Society of Japan Biological and Pharmaceutical Bulletin 1347-5215 40 6 941 944 20170000 10.1248/bpb.b17-00155 28566638 2-s2.0-85020052387 read0187881/published_papers/13781194 Sheuli Afroz Teru Ikoma Ayano Yagi Kentaro Kogure Akira Tokumura Tamotsu Tanaka Concentrated Phosphatidic Acid in Cereal Brans as Potential Protective Agents against Indomethacin-Induced Stomach Ulcer American Chemical Society Journal of Agricultural and Food Chemistry 1520-5118 64 37 6950 6957 20160921 10.1021/acs.jafc.6b02884 27561232 2-s2.0-84988649159 read0187881/published_papers/28144692 Sheuli Afroz Tern Ikoma Ayano Yagi Kentaro Kogure Akira Tokumura Tamotsu Tanaka Concentrated Phosphatidic Acid in Cereal Brans as Potential Protective Agents against Indomethacin-Induced Stomach Ulcer JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 1520-5118 64 37 6950 6957 20160900 10.1021/acs.jafc.6b02884 read0187881/published_papers/1851074 Chihiro Kamezaki Ami Nakashima Asako Yamada Sachiko Uenishi Hiroshi Ishibashi Natsumi Shibuya Susumu Hama Shinzo Hosoi Eiji Yamashita Kentaro Kogure Synergistic antioxidative effect of astaxanthin and tocotrienol by co-encapsulated in liposomes JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION 1880-5086 59 2 100 106 20160900 10.3164/jcbn.15-153 27698536 read0187881/published_papers/13781197 Shoko Itakura Susumu Hama Ryo Matsui Kentaro Kogure Effective cytoplasmic release of siRNA from liposomal carriers by controlling the electrostatic interaction of siRNA with a charge-invertible peptide, in response to cytoplasmic pH Royal Society of Chemistry Nanoscale 2040-3372 8 20 10649 10658 20160528 10.1039/c5nr08365f 27145993 2-s2.0-84971232259 read0187881/published_papers/28172564 Keita Takagi Takashi Ohgita Takenori Yamamoto Yasuo Shinohara Kentaro Kogure Transmission of External Environmental pH Information to the Inside of Liposomes via Pore-Forming Proteins Embedded within the Liposomal Membrane CHEMICAL & PHARMACEUTICAL BULLETIN 0009-2363 64 5 432 438 20160500 read0187881/published_papers/1851090 Mahadi Hasan Akinori Nishimoto Takashi Ohgita Susumu Hama Hiromu Kashida Hiroyuki Asanuma Kentaro Kogure Faint electric treatment-induced rapid and efficient delivery of extraneous hydrophilic molecules into the cytoplasm Elsevier B.V. Journal of Controlled Release 1873-4995 228 20 25 20160428 10.1016/j.jconrel.2016.02.048 26944781 2-s2.0-84961631654 read0187881/published_papers/26927274 Mahadi Hasan Akinori Nishimoto Takashi Ohgita Susumu Hama Hiromu Kashida Hiroyuki Asanuma Kentaro Kogure Faint electric treatment-induced rapid and efficient delivery of extraneous hydrophilic molecules into the cytoplasm JOURNAL OF CONTROLLED RELEASE 1873-4995 228 20 25 20160400 10.1016/j.jconrel.2016.02.048 26944781 2-s2.0-84961631654 read0187881/published_papers/24911530 Yamada A Mitsueda A Hasan M Ueda M Hama S Warashina S Nakamura T Harashima H Kogure K Tri-membrane nanoparticles produced by combining liposome fusion and a novel patchwork of bicelles to overcome endosomal and nuclear membrane barriers to cargo delivery. <p>Tri-membrane PD-MEND deliver cargo by sophisticated membrane fusions.</p> Royal Society of Chemistry (RSC) Biomaterials science 2047-4849 4 3 439 447 20160300 10.1039/c5bm00327j 26667208 read0187881/published_papers/1851104 Asako Yamada Asako Mitsueda Mahadi Hasan Miho Ueda Susumu Hama Shota Warashina Takashi Nakamura Hideyoshi Harashima Kentaro Kogure Tri-membrane nanoparticles produced by combining liposome fusion and a novel patchwork of bicelles to overcome endosomal and nuclear membrane barriers to cargo delivery Royal Society of Chemistry Biomaterials Science 2047-4849 4 3 439 447 20160301 10.1039/c5bm00327j 26667208 2-s2.0-84959111172 read0187881/published_papers/27247351 Ryouhei Yamashita Yumika Tabata Erina Iga Michiyasu Nakao Shigeki Sano Kentaro Kogure Akira Tokumura Tamotsu Tanaka Analysis of Molecular Species Profiles of Ceramide-1-phosphate and Sphingomyelin Using MALDI-TOF Mass Spectrometry LIPIDS 1558-9307 51 2 263 270 20160200 10.1007/s11745-015-4082-0 read0187881/published_papers/13781222 Ryouhei Yamashita Yumika Tabata Erina Iga Michiyasu Nakao Shigeki Sano Kentaro Kogure Akira Tokumura Tamotsu Tanaka Analysis of Molecular Species Profiles of Ceramide-1-phosphate and Sphingomyelin Using MALDI-TOF Mass Spectrometry Springer Verlag Lipids 1558-9307 51 2 263 270 20160201 10.1007/s11745-015-4082-0 26694604 2-s2.0-84957439211 read0187881/published_papers/26927275 Shoko Itakura Susumu Hama Ryo Matsui Kentaro Kogure Effective cytoplasmic release of siRNA from liposomal carriers by controlling the electrostatic interaction of siRNA with a charge-invertible peptide, in response to cytoplasmic pH NANOSCALE 2040-3372 8 20 10649 10658 20160000 10.1039/c5nr08365f 27145993 2-s2.0-84971232259 read0187881/published_papers/1797050 Mahadi Hasan Noriko Tarashima Koki Fujikawa Takashi Ohgita Susumu Hama Tamotsu Tanaka Hiroyuki Saito Noriaki Minakawa Kentaro Kogure The novel functional nucleic acid iRed effectively regulates target genes following cytoplasmic delivery by faint electric treatment SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS 1878-5514 17 1 554 562 20160000 10.1080/14686996.2016.1221726 27877903 read0187881/published_papers/13929687 喜田 孝史 木村 朱里 伊藤 葵 山下 量平 Kentaro Kogure 小暮 健太朗 Akira Tokumura 村 彰 Tamotsu Tanaka 田中 保 食品に含まれるグリコシルイノシトールホスホセラミドおよびフィトセラミド-1-リン酸 脂質栄養学 25 75 85 20160000 10.4010/jln.25.75 read0187881/published_papers/13781210 Keita Takagi Takashi Ohgita Takenori Yamamoto Yasuo Shinohara Kentaro Kogure Transmission of external environmental pH information to the inside of liposomes via pore-forming proteins embedded within the liposomal membrane Pharmaceutical Society of Japan Chemical and Pharmaceutical Bulletin 1347-5223 64 5 432 438 20160000 10.1248/cpb.c15-00985 27150475 2-s2.0-84969579933 read0187881/published_papers/1851123 Susumu Hama Shoko Itakura Mayumi Nakai Kayoko Nakayama Satoshi Morimoto Satoko Suzuki Kentaro Kogure Overcoming the polyethylene glycol dilemma via pathological environment-sensitive change of the surface property of nanoparticles for cellular entry JOURNAL OF CONTROLLED RELEASE 1873-4995 206 67 74 20150500 10.1016/j.jconrel.2015.03.011 read0187881/published_papers/1851125 Mao Toyoda Susumu Hama Yutaka Ikeda Yukio Nagasaki Kentaro Kogure Anti-cancer vaccination by transdermal delivery of antigen peptide-loaded nanogels via iontophoresis INTERNATIONAL JOURNAL OF PHARMACEUTICS 1873-3476 483 1-2 110 114 20150400 10.1016/j.ijpharm.2015.02.024 read0187881/published_papers/1851132 Shoko Itakura Susumu Hama Hisafumi Ikeda Naoto Mitsuhashi Eiji Majima Kentaro Kogure Effective capture of proteins inside living cells by antibodies indirectly linked to a novel cell-penetrating polymer-modified protein A derivative FEBS JOURNAL 1742-4658 282 1 142 152 20150100 10.1111/febs.13111 read0187881/published_papers/13781259 Ibuki Nakamura Susumu Hama Shoko Itakura Ichiro Takasaki Takayuki Nishi Yoshiaki Tabuchi Kentaro Kogure Lipocalin2 as a plasma marker for tumors with hypoxic regions SCIENTIFIC REPORTS 2045-2322 4 7235 null 20141200 10.1038/srep07235 read0187881/published_papers/1851135 Shoko Itakura Susumu Hama Takashi Ohgita Kentaro Kogure Development of Nanoparticles Incorporating a Novel Liposomal Membrane Destabilization Peptide for Efficient Release of Cargos into Cancer Cells PLOS ONE 1932-6203 9 10 e111181 null 20141000 10.1371/journal.pone.0111181 read0187881/published_papers/13781261 Takashi Nakamura Kouhei Ono Yoshiteru Suzuki Rumiko Moriguchi Kentaro Kogure Hideyoshi Harashima Octaarginine-Modified Liposomes Enhance Cross-Presentation by Promoting the C-Terminal Trimming of Antigen Peptide MOLECULAR PHARMACEUTICS 1543-8384 11 8 2787 2795 20140800 10.1021/mp500147y read0187881/published_papers/1851138 Yoshito Ikeda Hiroyuki Tsuchiya Susumu Hama Kazuaki Kajimoto Kentaro Kogure Resistin regulates the expression of plasminogen activator inhibitor-1 in 3T3-L1 adipocytes BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1090-2104 448 2 129 133 20140500 10.1016/j.bbrc.2014.03.076 read0187881/published_papers/29585219 Kentaro Kogure Biological and pharmaceutical bulletin: Foreword Biological and Pharmaceutical Bulletin 0918-6158 37 2 195 null 20140200 10.1248/bpb.b13-ctf3702 24492714 2-s2.0-84893289073 read0187881/published_papers/26927271 Susumu Hama Kentaro Kogure Nanoparticles Consisting of Tocopheryl Succinate Are a Novel Drug-Delivery System with Multifaceted Antitumor Activity BIOLOGICAL & PHARMACEUTICAL BULLETIN 0918-6158 37 2 196 200 20140200 10.1248/bpb.b13-00848 24492715 2-s2.0-84893224094 read0187881/published_papers/37403375 Kogure Kentaro 小暮 健太朗 Report on the Symposium on APSTJ2025 APSTJ2025シンポジウム「バイオとナノが拓く医療イノベーション」開催報告 The Academy of Pharmaceutical Science and Technology, Japan Journal of Pharmaceutical Science and Technology, Japan 薬剤学 0372-7629 74 4 287 288 20140000 10.14843/jpstj.74.287 read0187881/published_papers/1851152 Susumu Hama Yuki Kimura Aya Mikami Kanako Shiota Mao Toyoda Atsushi Tamura Yukio Nagasaki Kiyoshi Kanamura Kazuaki Kajimoto Kentaro Kogure Electric Stimulus Opens Intercellular Spaces in Skin JOURNAL OF BIOLOGICAL CHEMISTRY 1083-351X 289 4 2450 2456 20140100 10.1074/jbc.M113.514414 read0187881/published_papers/1851156 Takashi Ohgita Naoki Hayashi Naomasa Gotoh Kentaro Kogure Suppression of type III effector secretion by polymers OPEN BIOLOGY 2046-2441 3 12 130133 null 20131200 10.1098/rsob.130133 read0187881/published_papers/1851176 Asako Mitsueda Yuri Shimatani Masahiro Ito Takashi Ohgita Asako Yamada Susumu Hama Astrid Graslund Staffan Lindberg Ulo Langel Hideyoshi Harashima Ikuhiko Nakase Shiroh Futaki Kentaro Kogure Development of a Novel Nanoparticle by Dual Modification With the Pluripotential Cell-Penetrating Peptide PepFect6 for Cellular Uptake, Endosomal Escape, and Decondensation of an siRNA Core Complex BIOPOLYMERS 1097-0282 100 6 698 704 20131100 10.1002/bip.22310 read0187881/published_papers/1851162 Yoshito Ikeda Hiroyuki Tsuchiya Susumu Hama Kazuaki Kajimoto Kentaro Kogure Resistin affects lipid metabolism during adipocyte maturation of 3T3-L1 cells FEBS JOURNAL 1742-4658 280 22 5884 5895 20131100 10.1111/febs.12514 read0187881/published_papers/29571013 Hiroyuki Tsuchiya Kentaro Kogure Goshi Shiota Reply Hepatology 0270-9139 58 3 1186 null 20130900 10.1002/hep.26217 23280494 2-s2.0-84883242593 read0187881/published_papers/13948707 Arisa Kawahara 小暮 健太朗 Naoko Haraguchi Hiroyuki Tsuchiya Yoshito Ikeda Susumu Hama Kentaro Kogure Peroxisome Proliferator-Activated Receptor gamma (PPAR gamma)-Independent Specific Cytotoxicity against Immature Adipocytes Induced by PPAR gamma Antagonist T0070907 Peroxisome proliferator-activated receptor(PPAR)γ-independent specific cytotoxicity against immature adipocytes induced by PPARγ antagonist T0070907 BIOLOGICAL & PHARMACEUTICAL BULLETIN Biol. Pharm. Bull 0918-6158 36 9 1428 1434 20130900 10.1248/bpb.b13-00024 read0187881/published_papers/13948713 Takanori Tsukamoto Susumu Hama Kentaro Kogure Hiroyuki Tsuchiya Selenate induces epithelial-mesenchymal transition in a colorectal carcinoma cell line by ART activation EXPERIMENTAL CELL RESEARCH 0014-4827 319 13 1913 1921 20130800 10.1016/j.yexcr.2013.05.031 read0187881/published_papers/29564558 Takashi Ohgita Naoki Hayashi Susumu Hama Hiroyuki Tsuchiya Naomasa Gotoh Kentaro Kogure A novel effector secretion mechanism based on proton-motive force-dependent type III secretion apparatus rotation FASEB Journal 1530-6860 27 7 2862 2872 20130700 10.1096/fj.13-229054 23515444 2-s2.0-84879650647 read0187881/published_papers/1851178 Takashi Ohgita Naoki Hayashi Susumu Hama Hiroyuki Tsuchiya Naomasa Gotoh Kentaro Kogure A novel effector secretion mechanism based on proton-motive force-dependent type III secretion apparatus rotation FASEB JOURNAL 1530-6860 27 7 2862 2872 20130700 10.1096/fj.13-229054 read0187881/published_papers/13948715 Yu Ebata Junichi Takino Hiroyuki Tsuchiya Tomohiko Sakabe Yoshito Ikeda Susumu Hama Kentaro Kogure Masayoshi Takeuchi Goshi Shiota Presence of Glyceraldehyde-Derived Advanced Glycation End-Products in the Liver of Insulin-Resistant Mice INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH 1664-2821 83 2 137 141 20130400 10.1024/0300-9831/a000150 read0187881/published_papers/13948730 Hiroyuki Tsuchiya Yu Ebata Tomohiko Sakabe Susumu Hama Kentaro Kogure Goshi Shiota High-fat, high-fructose diet induces hepatic iron overload via a hepcidin-independent mechanism prior to the onset of liver steatosis and insulin resistance in mice METABOLISM-CLINICAL AND EXPERIMENTAL 0026-0495 62 1 62 69 20130100 10.1016/j.metabol.2012.06.008 read0187881/published_papers/13948726 Osamu Yoshikawa Yu Ebata Hiroyuki Tsuchiya Arisa Kawahara Chihiro Kojima Yoshito Ikeda Susumu Hama Kentaro Kogure Koichi Shudo Goshi Shiota A Retinoic Acid Receptor Agonist Tamibarotene Suppresses Iron Accumulation in the Liver OBESITY 1930-7381 21 1 E22 E25 20130100 10.1002/oby.20013 read0187881/published_papers/13948719 Takashi Nakamura Rumiko Moriguchi Kentaro Kogure Hideyoshi Harashima Incorporation of polyinosine-polycytidylic acid enhances cytotoxic T cell activity and antitumor effects by octaarginine-modified liposomes encapsulating antigen, but not by octaarginine-modified antigen complex INTERNATIONAL JOURNAL OF PHARMACEUTICS 0378-5173 441 1-2 476 481 20130100 10.1016/j.ijpharm.2012.11.006 read0187881/published_papers/1851181 Susumu Hama Sachiko Uenishi Asako Yamada Takashi Ohgita Hiroyuki Tsuchiya Eiji Yamashita Kentaro Kogure Scavenging of Hydroxyl Radicals in Aqueous Solution by Astaxanthin Encapsulated in Liposomes BIOLOGICAL & PHARMACEUTICAL BULLETIN 0918-6158 35 12 2238 2242 20121200 10.1248/bpb.b12-00715 read0187881/published_papers/13948747 Hiroyuki Tsuchiya Yoshito Ikeda Yu Ebata Chihiro Kojima Rikutaro Katsuma Tatsuaki Tsuruyama Tomohiko Sakabe Kohei Shomori Noriko Komeda Shoko Oshiro Hideharu Okamoto Kazuko Takubo Susumu Hama Koichi Shudo Kentaro Kogure Goshi Shiota Retinoids ameliorate insulin resistance in a leptin-dependent manner in mice HEPATOLOGY 0270-9139 56 4 1319 1330 20121000 10.1002/hep.25798 read0187881/published_papers/1851187 Susumu Hama Satoru Utsumi Yuki Fukuda Kayoko Nakayama Yuriko Okamura Hiroyuki Tsuchiya Kenji Fukuzawa Hedeyoshi Harashima Kentaro Kogure Development of a novel drug delivery system consisting of an antitumor agent tocopheryl succinate JOURNAL OF CONTROLLED RELEASE 0168-3659 161 3 843 851 20120800 10.1016/j.jconrel.2012.05.031 read0187881/published_papers/13948785 S. Hama M. Arata I. Nakamura T. Kasetani S. Itakura H. Tsuchiya T. Yoshiki K. Kogure Prevention of tumor growth by needle-free jet injection of anti-C7orf24 siRNA CANCER GENE THERAPY 0929-1903 19 8 553 557 20120800 10.1038/cgt.2012.31 read0187881/published_papers/13948759 Susumu Hama Kanako Takahashi Yuko Inai Kanako Shiota Ryota Sakamoto Asako Yamada Hiroyuki Tsuchiya Kiyoshi Kanamura Eiji Yamashita Kentaro Kogure Protective effects of topical application of a poorly soluble antioxidant astaxanthin liposomal formulation on ultraviolet-induced skin damage JOURNAL OF PHARMACEUTICAL SCIENCES 0022-3549 101 8 2909 2916 20120800 10.1002/jps.23216 read0187881/published_papers/13948753 Katsuma Kitazoe Yeon-Su Park Noritada Kaji Yukihiro Okamoto Manabu Tokeshi Kentaro Kogure Hideyoshi Harashima Yoshinobu Baba Fabrication of Functionalized Double-Lamellar Multifunctional Envelope-Type Nanodevices Using a Microfluidic Chip with a Chaotic Mixer Array PLOS ONE 1932-6203 7 6 e39057 null 20120600 10.1371/journal.pone.0039057 read0187881/published_papers/1851190 Kaoru Kigasawa Moeko Miyashita Kazuaki Kajimoto Kiyoshi Kanamura Hideyoshi Harashima Kentaro Kogure Efficient Intradermal Delivery of Superoxide Dismutase Using a Combination of Liposomes and Iontophoresis for Protection against UV-Induced Skin Damage BIOLOGICAL & PHARMACEUTICAL BULLETIN 0918-6158 35 5 781 785 20120500 10.1248/bpb.35.781 read0187881/published_papers/35353616 小暮 健太朗 濱 進 福澤 健治 2.トコフェロールコハク酸の抗腫瘍効果とDDSへの展開(第23回研究会研究発表要旨,ビタミンE研究会) 公益社団法人 日本ビタミン学会 ビタミン 86 5 331 331 20120000 10.20632/vso.86.5-6_331 read0187881/published_papers/24836798 Ikuhiko Nakase Hidetaka Akita Kentaro Kogure Astrid Gräslund Ulo Langel Hideyoshi Harashima Shiroh Futaki (Review) Efficient intracellular delivery of nucleic acid pharmaceuticals using cell-penetrating peptides Over the last 20 years, researchers have designed or discovered peptides that can permeate membranes and deliver exogenous molecules inside a cell. These peptides, known as cell-penetrating peptides (CPPs), typically consist of 6-30 residues, including HIV TAT peptide, penetratin, oligoarginine, transportan, and TP10. Through chemical conjugation or noncovalent complex formation, these structures successfully deliver bioactive and membrane-impermeable molecules into cells. CPPs have also gained attention as an attractive vehicle for the delivery of nucleic acid pharmaceuticals (NAPs), including genes/plasmids, short oligonucleotides, and small interference RNAs and their analogues, due to their high internalization efficacy, low cytotoxicity, and flexible structural design. In this Account, we survey the potential of CPPs for the design and optimization of NAP delivery systems. First, we describe the impact of the N-terminal stearylation of CPPs. Endocytic pathways make a major contribution to the cellular uptake of NAPs. Stearylation at the N-terminus of CPPs with stearyl-octaarginine (R8), stearyl-(RxR)(4), and stearyl-TP10 prompts the formation of a self-assembled core-shell nanoparticle with NAPs, a compact structure that promotes cellular uptake. Researchers have designed modifications such as the addition of trifluoromethylquinoline moieties to lysine residues to destabilize endosomes, as exemplified by PepFect 6, and these changes further improve biological responsiveness. Alternatively, stearylation also allows implantation of CPPs onto the surface of liposomes. This feature facilitates "programmed packaging" to establish multifunctional envelope-type nanodevices (MEND). The R8-MEND showed high transfection efficiency comparable to that of adenovirus in non-dividing cells. Understanding the cellular uptake mechanisms of CPPs will further improve CPP-mediated NAP delivery. The cellular uptake of CPPs and their NAP complex involves various types of endocytosis. Macropinocytosis, a mechanism which is also activated in response to stimuli such as growth factors or viruses, is a primary pathway for arginine-rich CPPs because high cationic charge density promotes this endocytic pathway. The use of larger endosomes (known as macropinosomes) rather than clathrin- or caveolae-mediated endocytosis has been reported in macropinocytosis which would also facilitate the endocytosis of NAP nanoparticles into cells. Accounts of Chemical Research 45 7 1132 1139 20120000 10.1021/ar200256e 22208383 read0187881/published_papers/13948812 Yoshito Ikeda Susumu Hama Kazuaki Kajimoto Takashi Okuno Hiroyuki Tsuchiya Kentaro Kogure Quantitative Comparison of Adipocytokine Gene Expression during Adipocyte Maturation in Non-obese and Obese Rats BIOLOGICAL & PHARMACEUTICAL BULLETIN 0918-6158 34 6 865 870 20110600 10.1248/bpb.34.865 read0187881/published_papers/13948824 Takashi Ohgita Takashi Okuno Susumu Hama Hiroyuki Tsuchiya Kentaro Kogure A New Method to Evaluate the Unfolding Activity of Chaperone Unit ClpA Based on Fe-S Cluster Disruption CHEMICAL & PHARMACEUTICAL BULLETIN 0009-2363 59 5 657 661 20110500 10.1248/cpb.59.657 read0187881/published_papers/1851354 Kaoru Kigasawa Kazuaki Kajimoto Takashi Nakamura Susumu Hama Kiyoshi Kanamura Hideyoshi Harashima Kentaro Kogure Noninvasive and efficient transdermal delivery of CpG-oligodeoxynucleotide for cancer immunotherapy JOURNAL OF CONTROLLED RELEASE 0168-3659 150 3 256 265 20110300 10.1016/j.jconrel.2011.01.018 read0187881/published_papers/25556799 H. Akita Akita H K. Kogure Kogure K R. Moriguchi Moriguchi R Y. Nakamura Nakamura Y T. Higashi Higashi T T. Nakamura Nakamura T S. Serada Serada S M. Fujimoto Fujimoto M T. Naka Naka T S. Futaki Futaki S H. Harashima Harashima H Reprint of: Nanoparticles for ex vivo siRNA delivery to dendritic cells for cancer vaccines: Programmed endosomal escape and dissociation Reprint of: Nanoparticles for ex vivo siRNA delivery to dendritic cells for cancer vaccines: Programmed endosomal escape and dissociation. Journal of Controlled Release Journal of controlled release : official journal of the Controlled Release Society 0168-3659 149 1 58 64 20110105 10.1016/j.jconrel.2010.08.023 20826039 read0187881/published_papers/38061130 Ohgita Takashi Sasa Tatsunori Okuno Takashi Hama Susumu Tsuchiya Hiroyuki Kogure Kentaro 2D1636 Development of an improved method for evaluating the unfolding activity of Escherichia coli ClpA by using Ferredoxin as a model substrate(Protein: Structure & Function 1,The 48th Annual Meeting of the Biophysical Society of Japan) 2D1636 Ferredoxinをモデル基質とする大腸菌シャペロンClpAの新規アンフォールディング活性評価法の確立(蛋白質_構造機能相関1,第49回日本生物物理学会年会) The Biophysical Society of Japan General Incorporated Association 一般社団法人 日本生物物理学会 Seibutsu Butsuri 生物物理 51 S81 null 20110000 10.2142/biophys.51.S81_4 read0187881/published_papers/24836797 Ikuhiko Nakase Kentaro Kogure Hideyoshi Harashima Shiroh Futaki (Review) Application of a fusiogenic peptide GALA for intracellular delivery Methods in Molecular Biology 683 525 533 20110000 read0187881/published_papers/1851362 Kazuaki Kajimoto Masahiko Yamamoto Misuzu Watanabe Kaoru Kigasawa Kiyoshi Kanamura Hideyoshi Harashima Kentaro Kogure Noninvasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis INTERNATIONAL JOURNAL OF PHARMACEUTICS 0378-5173 403 1-2 57 65 20110100 10.1016/j.ijpharm.2010.10.021 read0187881/published_papers/13948800 Katsuma Kitazoe Jun Wang Noritada Kaji Yukihiro Okamoto Manabu Tokeshi Kentaro Kogure Hideyoshi Harashima Yoshinobu Baba A touch-and-go lipid wrapping technique in microfluidic channels for rapid fabrication of multifunctional envelope-type gene delivery nanodevices LAB ON A CHIP 1473-0189 11 19 3256 3262 20110000 10.1039/c1lc20392d read0187881/published_papers/13948834 Takashi Okuno Takashi Ohgita Tatsunori Sasa Aoi Nonaka Noriaki Funasaki Kentaro Kogure Fluorescence Polarization Analysis for Revealing Molecular Mechanism of Nucleotide-Dependent Phospholipid Membrane Binding of MinD Adenosine 5 '-Triphosphate, Adenosine Triphosphatase BIOLOGICAL & PHARMACEUTICAL BULLETIN 0918-6158 33 10 1746 1750 20101000 10.1248/bpb.33.1746 read0187881/published_papers/13948841 Hidetaka Akita Kentaro Kogure Rumiko Moriguchi Yoshio Nakamura Tomoko Higashi Takashi Nakamura Satoshi Serada Minoru Fujimoto Tetsuji Naka Shiroh Futaki Hideyoshi Harashima Nanoparticles for ex vivo siRNA delivery to dendritic cells for cancer vaccines: Programmed endosomal escape and dissociation JOURNAL OF CONTROLLED RELEASE 0168-3659 143 3 311 317 20100500 10.1016/j.jconrel.2010.01.012 read0187881/published_papers/13948850 Takashi Okuno Hiroko Yamabayashi Kentaro Kogure Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins MOLECULAR BIOLOGY REPORTS 0301-4851 37 3 1165 1168 20100300 10.1007/s11033-009-9477-7 read0187881/published_papers/26160823 Hatakeyama H Akita H Kogure K Harashima H A novel nonviral gene delivery system: multifunctional envelope-type nano device. In this review we introduce a new concept for developing a nonviral gene delivery system which we call "Programmed Packaging." Based on this concept, we succeeded in developing a multifunctional envelope-type nano device (MEND), which exerts high transfection activities equivalent to those of an adenovirus in a dividing cell. The use of MEND has been extended to in vivo applications. PEG/peptide/DOPE ternary conjugate (PPD)-MEND, a new in vivo gene delivery system for the targeting of tumor cells that dissociates surface-modified PEG in tumor tissue by matrix metalloproteinase (MMP) and exerts significant transfection activities, was developed. In parallel with the development of MEND, a quantitative gene delivery system, Confocal Image-assisted 3-dimensionally integrated quantification (CIDIQ), also was developed. This method identified the rate-limiting step of the nonviral gene delivery system by comparing it with adenoviral-mediated gene delivery. The results of this analysis provide a new direction for the development of rational nonviral gene delivery systems. Advances in biochemical engineering/biotechnology 0724-6145 119 197 230 20100000 10.1007/10_2008_40 19343308 read0187881/published_papers/1851364 K. Kigasawa K. Kajimoto S. Hama A. Saito K. Kanamura K. Kogure Noninvasive delivery of siRNA into the epidermis by iontophoresis using an atopic dermatitis-like model rat INTERNATIONAL JOURNAL OF PHARMACEUTICS 0378-5173 383 1-2 157 160 20100100 10.1016/j.ijpharm.2009.08.036 read0187881/published_papers/13948861 Takashi Okuno Machiko Ogoh Hiromitsu Tanina Noriaki Funasaki Kentaro Kogure Direct Monitoring of Interaction between Escherichia coli Proteins, MinC and Monomeric FtsZ, in Solution BIOLOGICAL & PHARMACEUTICAL BULLETIN 0918-6158 32 8 1473 1475 20090800 10.1248/bpb.32.1473 read0187881/published_papers/13948866 Hidetaka Akita Asako Kudo Arisa Minoura Masaya Yamaguti Ikrarny A. Khalil Rumiko Moriguchi Tomoya Masuda Radostin Danev Kuniaki Nagayama Kentaro Kogure Hideyoshi Harashima Multi-layered nanoparticles for penetrating the endosome and nuclear membrane via a step-wise membrane fusion process BIOMATERIALS 0142-9612 30 15 2940 2949 20090500 10.1016/j.biomaterials.2009.02.009 read0187881/published_papers/13948863 Atthachai Homhuan Kentaro Kogure Takashi Nakamura Nilabh Shastri Hideyoshi Harashima Enhanced antigen presentation and CTL activity by transduction of mature rather than immature dendritic cells with octaarginine-modified liposomes JOURNAL OF CONTROLLED RELEASE 0168-3659 136 1 79 85 20090500 10.1016/j.jconrel.2009.01.004 read0187881/published_papers/13948870 Kaoru Kigasawa Kazuaki Kajimoto Misuzu Watanabe Kiyoshi Kanamura Akiyoshi Saito Kentaro Kogure In Vivo Transdermal Delivery of Diclofenac by Ion-Exchange Iontophoresis with Geraniol BIOLOGICAL & PHARMACEUTICAL BULLETIN 0918-6158 32 4 684 687 20090400 10.1248/bpb.32.684 read0187881/published_papers/13948872 Akira Joraku 小暮 健太朗 Atthachai Homhuan Koji Kawai Takahiro Yamamoto Jun Miyazaki Kentaro Kogure Ikuya Yano Hideyoshi Harashima Hideyuki Akaza Immunoprotection against murine bladder carcinoma by octaarginine-modified liposomes incorporating cell wall of Mycobacterium bovis bacillus Calmette-GuErin Immunoprotection against murine bladder carcinoma by octaarginine-modified liposomes incorporating cell wall of Mycobacterium bovis bacillus Calmette-Guérin BJU INTERNATIONAL BJU Int. 1464-4096 103 5 686 693 20090300 10.1111/j.1464-410X.2008.08235.x read0187881/published_papers/13948912 Rumiko Moriguchi Kentaro Kogure Hideyoshi Harashima Non-linear pharmacodynamics in the transfection efficiency of a non-viral gene delivery system INTERNATIONAL JOURNAL OF PHARMACEUTICS 0378-5173 363 1-2 192 198 20081100 10.1016/j.ijpharm.2008.07.020 read0187881/published_papers/13948874 Kenichi Niikura Shota Sekiguchi Takashi Nishio Tomoya Masuda Hidetaka Akita Yasutaka Matsuo Kentaro Kogure Hideyoshi Harashima Kuniharu Ijiro Oligosaccharide-Mediated Nuclear Transport of Nanoparticles CHEMBIOCHEM 1439-4227 9 16 2623 2627 20081100 10.1002/cbic.200800464 read0187881/published_papers/13948906 Atsushi Yamashita Daizo Kanda Ryo Katoono Nobuhiko Yui Tooru Ooya Atsushi Maruyama Hidetaka Akita Kentaro Kogure Hideyoshi Harashima Supramolecular control of polyplex dissociation and cell transfection: Efficacy of amino groups and threading cyclodextrins in biocleavable polyrotaxanes JOURNAL OF CONTROLLED RELEASE 0168-3659 131 2 137 144 20081000 10.1016/j.jconrel.2008.07.011 read0187881/published_papers/13948955 Kentaro Sasaki Kentaro Kogure Shinji Chaki Yoshio Nakamura Rumiko Moriguchi Hirofumi Hamada Radostin Danev Kuniaki Nagayama Shiroh Futaki Hideyoshi Harashima An artificial virus-like nano carrier system: enhanced endosomal escape of nanoparticles via synergistic action of pH-sensitive fusogenic peptide derivatives ANALYTICAL AND BIOANALYTICAL CHEMISTRY 1618-2642 391 8 2717 2727 20080800 10.1007/s00216-008-2012-1 read0187881/published_papers/13948939 Hiroshi Kuramoto Yeon-Su Park Noritada Kaji Manabu Tokeshi Kentaro Kogure Yasuo Shinohara Hideyoshi Harashima Yoshinobu Baba On-chip fabrication of mutifunctional envelope-type nanodevices for gene delivery ANALYTICAL AND BIOANALYTICAL CHEMISTRY 1618-2650 391 8 2729 2733 20080800 10.1007/s00216-008-2124-7 read0187881/published_papers/13948928 Takashi Nakamura Rumiko Moriguchi Kentaro Kogure Nilabh Shastri Hideyoshi Harashima Efficient MHC class I presentation by controlled intracellular trafficking of antigens in octaarginine-modified liposomes MOLECULAR THERAPY 1525-0016 16 8 1507 1514 20080800 10.1038/mt.2008.122 read0187881/published_papers/13948919 Ayman El-Sayed Ikramy A. Khalil Kentaro Kogure Shiroh Futaki Hideyoshi Harashima Octaarginine- and octalysine-modified nanoparticles have different modes of endosomal escape JOURNAL OF BIOLOGICAL CHEMISTRY 0021-9258 283 34 23450 23461 20080800 10.1074/jbc.M709387200 read0187881/published_papers/25294855 I.A. Khalil Khalil IA K. Kogure Kogure K S. Futaki Futaki S H. Harashima Harashima H Octaarginine-modified liposomes: Enhanced cellular uptake and controlled intracellular trafficking Octaarginine-modified liposomes: enhanced cellular uptake and controlled intracellular trafficking. International Journal of Pharmaceutics International journal of pharmaceutics 0378-5173 354 1-2 39 48 20080416 10.1016/j.ijpharm.2007.12.003 18242018 read0187881/published_papers/26295448 Kogure K Akita H Yamada Y Harashima H Multifunctional envelope-type nano device (MEND) as a non-viral gene delivery system. In this review, we describe a key role of octaarginine (R8) in developing our new concept of "Programmed Packaging", by which we succeeded in creating a multifunctional envelope-type nano device (MEND) as a non-viral gene-delivery system. This concept can be applied not only to nuclear targeting of plasmid DNA (pDNA) but also to cytosolic delivery of functional nucleic acids such as oligonucleotides or siRNA. This concept has been extended to other organelles such as mitochondria as a foundation for innovative nanomedicine. Finally, we discuss the rate-limiting step in gene delivery by comparing non-viral and viral gene delivery systems, which clearly indicates the importance of nuclear disposition of pDNA for efficient transfection. 4-5 Advanced drug delivery reviews 0169-409X 60 4-5 559 571 20080300 10.1016/j.addr.2007.10.007 18037529 read0187881/published_papers/26102363 Kentaro Kogure Hidetaka Akita Hiroyuki Kamiya Hideyoshi Harashima Programmed Packaging: A New Drug Delivery System and its Application to Gene Therapy Modern Biopharmaceuticals: Design, Development and Optimization 4 1521 1536 20080205 10.1002/9783527620982.ch63 2-s2.0-34247170389 read0187881/published_papers/34633296 Yuma Yamada Hidetaka Akita Hiroyuki Kamiya Kentaro Kogure Takenori Yamamoto Yasuo Shinohara Kikuji Yamashita Hideo Kobayashi Hiroshi Kikuchi Hideyoshi Harashima MITO-Porter: A liposome-based carrier system for delivery of macromolecules into mitochondria via membrane fusion. Mitochondria are the principal producers of energy in higher cells. Mitochondrial dysfunction is implicated in a variety of human diseases, including cancer and neurodegenerative disorders. Effective medical therapies for such diseases will ultimately require targeted delivery of therapeutic proteins or nucleic acids to the mitochondria, which will be achieved through innovations in the nanotechnology of intracellular trafficking. Here we describe a liposome-based carrier that delivers its macromolecular cargo to the mitochondrial interior via membrane fusion. These liposome particles, which we call MITO-Porters, carry octaarginine surface modifications to stimulate their entry into cells as intact vesicles (via macropinocytosis). We identified lipid compositions for the MITO-Porter which promote both its fusion with the mitochondrial membrane and the release of its cargo to the intra-mitochondrial compartment in living cells. Thus, the MITO-Porter holds promise as an efficacious system for the delivery of both large and small therapeutic molecules into mitochondria. Biochimica et biophysica acta 0006-3002 1778 2 423 32 20080200 18054323 read0187881/published_papers/13948959 Tomoya Masuda Hidetaka Akita Takashi Nishio Kenichi Niikura Kentaro Kogure Kuniharu Ijiro Hideyoshi Harashima Development of lipid particles targeted via sugar-lipid conjugates as novel nuclear gene delivery system BIOMATERIALS 0142-9612 29 6 709 723 20080200 10.1016/j.biomaterials.2007.09.039 read0187881/published_papers/13929688 Yuma Yamada Hidetaka Akita Hiroyuki Kamiya Kentaro Kogure Takenori Yamamoto Yasuo Shinohara Kikuji Yamashita Hideo Kobayashi Hiroshi Kikuchi Hideyoshi Harashima MITO-Porter: A liposome-based carrier system for delivery of macromolecules into mitochondria via membrane fusion BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 0005-2736 1778 2 423 432 20080200 10.1016/j.bbamem.2007.11.002 18054323 read0187881/published_papers/38166498 Yamashita A Kanda D Katoono R Yui N Ooya T Maruyama A Akita H Kogure K Harashima H Supramolecular control of polyplex dissociation and cell transfection: Efficacy of amine and threading cyclodextrin in biocleavable polyrotaxanes 8th World Biomaterials Congress 2008 4 null null 20080000 2-s2.0-84869062833 read0187881/published_papers/25294913 El-Sayed Ayman Khalil Ikramy A Kogure Kentaro Futaki Shiroh Harashima Hideyoshi Effect of surface-modification with octaarginine versus octalysine on cellular uptake and endosomal escape of nanoparticles YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 0031-6903 128 111 112 20080000 read0187881/published_papers/28805564 Kentaro Kogure Hidetaka Akita Hideyoshi Harashima Multifunctional envelope-type nano device for non-viral gene delivery: Concept and application of Programmed Packaging JOURNAL OF CONTROLLED RELEASE 0168-3659 122 3 246 251 20071000 10.1016/j.jconrel.2007.06.018 read0187881/published_papers/25294853 A. Homhuan Homhuan A K. Kogure Kogure K H. Akaza Akaza H S. Futaki Futaki S T. Naka Naka T Y. Fujita Fujita Y I. Yano Yano I H. Harashima Harashima H New packaging method of mycobacterial cell wall using octaarginine-modified liposomes: Enhanced uptake by and immunostimulatory activity of dendritic cells New packaging method of mycobacterial cell wall using octaarginine-modified liposomes: enhanced uptake by and immunostimulatory activity of dendritic cells. Journal of Controlled Release Journal of controlled release : official journal of the Controlled Release Society 0168-3659 120 1-2 60 69 20070713 10.1016/j.jconrel.2007.03.017 17467840 read0187881/published_papers/25294851 Y. Nakamura Nakamura Y K. Kogure Kogure K S. Futaki Futaki S H. Harashima Harashima H Octaarginine-modified multifunctional envelope-type nano device for siRNA Octaarginine-modified multifunctional envelope-type nano device for siRNA. Journal of Controlled Release Journal of controlled release : official journal of the Controlled Release Society 0168-3659 119 3 360 367 20070622 10.1016/j.jconrel.2007.03.010 17478000 read0187881/published_papers/25294799 I.A. Khalil K. Kogure S. Futaki S. Hama H. Akita M. Ueno H. Kishida M. Kudoh Y. Mishina K. Kataoka M. Yamada H. Harashima Octaarginine-modified multifunctional envelope-type nanoparticles for gene delivery Gene Therapy 14 8 682 689 20070400 10.1038/sj.gt.3302910 read0187881/published_papers/25863031 Kenichi Niikura Takashi Nishio Hidetaka Akita Yasutaka Matsuo Ryosuke Kamitani Kentaro Kogure Hideyoshi Harashima Kuniharu Ijiro Accumulation of O-GlcNAc-displaying CdTe Quantum Dots in Cells in the presence of ATP ChemBioChem 1439-7633 8 4 379 384 20070100 10.1002/cbic.200600496 read0187881/published_papers/25340614 Hatakeyama, H Akita, H Kogure, K Oishi, M Nagasaki, Y Kihira, Y Ueno, M Kobayashi, H Kikuchi, H Harashima, H Development of a novel systemic gene delivery system for cancer therapy with a tumor-specific cleavable PEG-lipid NATURE PUBLISHING GROUP GENE THERAPY 0969-7128 14 1 68 77 20070100 10.1038/sj.gt.3302843 read0187881/published_papers/25173175 Yamashita A Yui N Ooya T Kano A Maruyama A Akita H Kogure K Harashima H Synthesis of a biocleavable polyrotaxane-plasmid DNA (pDNA) polyplex and its use for the rapid nonviral delivery of pDNA to cell nuclei Nature Protocols 1 6 2861 2869 20070000 10.1038/nprot.2006.438 2-s2.0-34548848796 read0187881/published_papers/34633302 Akitada Iwasa Hidetaka Akita Ikramy Khalil Kentaro Kogure Shiroh Futaki Hideyoshi Harashima Cellular uptake and subsequent intracellular trafficking of R8-liposomes introduced at low temperature. Intracellular trafficking is a determining factor in the transgene expression efficiency of gene vectors. In the present study, the mechanism of the cellular uptake of octaarginine (R8)-modified liposomes, when introduced at 37 degrees C and 4 degrees C, was investigated in living cells. Compared with 37 degrees C, the uptake of R8-liposomes was only slightly reduced at 4 degrees C. Dual imaging of liposomes and plasma membranes revealed that R8-liposomes were internalized by vesicular transport, and partially escaped to the cytosol at the perinuclear region at 37 degrees C. When introduced at 4 degrees C, intracellular liposomes were observed within a specific region close to the plasma membrane, and internalization of the plasma membrane was completely inhibited. Therefore, at 4 degrees C, R8-liposomes appear to enter cells via unique pathway, which is separate and distinct from energy-dependent vesicular transport. The subsequent nuclear delivery of encapsulated pDNA, when introduced at 4 degrees C, was less prominent compared with those introduced at 37 degrees C. Collectively, these findings demonstrate that a vesicular transport-independent pathway is responsible for the cellular uptake of liposomes. In addition, the uptake route is closely related to the subsequent nuclear delivery process; the operation of an endogenous vesicular sorting system is advantageous for the nuclear delivery of pDNA. Biochimica et biophysica acta 0006-3002 1758 6 713 20 20060600 16780792 read0187881/published_papers/25294868 A. Iwasa Iwasa Akitada H. Akita Akita Hidetaka I. Khalil Khalil Ikramy K. Kogure Kogure Kentaro S. Futaki Futaki Shiroh H. Harashima Harashima Hideyoshi Cellular uptake and subsequent intracellular trafficking of R8-liposomes introduced at low temperature Biochimica et Biophysica Acta - Biomembranes BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 0005-2736 1758 6 713 720 20060600 10.1016/j.bbamem.2006.04.015 read0187881/published_papers/25294880 Kogure Kentaro Nakamura Yoshio Khalil Ikramy A Futaki Shiroh Harashima Hideyoshi Significant Silencing Effect of a Multifunctional Envelope-Type Nano Device Encapsulating Antisense Oligodeoxynucleotide and Double-Stranded siRNA MOLECULAR THERAPY 1525-0016 13 S202 S203 20060500 10.1016/j.ymthe.2006.08.599 read0187881/published_papers/25294878 Iwasa Akitada Akita Hidetaka Khalil Ikramy Kogure Kentaro Futaki Shiroh Harashima Hideyoshi Cellular Uptake and Subsequent Intracellular of R8-Liposomes Introduced at Low Temperature MOLECULAR THERAPY 1525-0016 13 S129 null 20060500 10.1016/j.ymthe.2006.08.397 read0187881/published_papers/26160827 Khalil IA Kogure K Akita H Harashima H Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery. The successful delivery of therapeutic genes to the designated target cells and their availability at the intracellular site of action are crucial requirements for successful gene therapy. Nonviral gene delivery is currently a subject of increasing attention because of its relative safety and simplicity of use; however, its use is still far from being ideal because of its comparatively low efficiency. Most of the currently available nonviral gene vectors rely on two main components, cationic lipids and cationic polymers, and a variety of functional devices can be added to further optimize the systems. The design of these functional devices depends mainly on our understanding of the mechanisms involved in the cellular uptake and intracellular disposition of the therapeutic genes as well as their carriers. Macromolecules are internalized into cells by a variety of mechanisms, and their intracellular fate is usually linked to the entry mechanism. Therefore, the successful design of a nonviral gene delivery system requires a deep understanding of gene/carrier interactions as well as the mechanisms involved in the interaction of the systems with the target cells. In this article, we review the different uptake pathways that are involved in nonviral gene delivery from a gene delivery point of view. In addition, available knowledge concerning cellular entry and the intracellular trafficking of cationic lipid-DNA complexes (lipoplexes) and cationic polymer-DNA complexes (polyplexes) is summarized. Pharmacological reviews 0031-6997 58 1 32 45 20060300 10.1124/pr.58.1.8 16507881 read0187881/published_papers/25294798 I.A. Khalil K. Kogure S. Futaki H. Harashima High density of octaarginine stimulates macropinocytosis leading to efficient intracellular trafficking for gene expression Journal of Biological Chemistry 281 6 3544 3551 20060210 10.1074/jbc.M503202200 read0187881/published_papers/26160829 Kogure K Akita H Harashima H [Envelope-type nano device for gene delivery]. We introduced a new concept "Programmed Packaging" for the rational development of non viral gene delivery system. There are many non-viral gene delivery system, however, their utility is limited in the in vitro experiments. The major reason is considered that it is difficult to package many functional devices into a nanoparticle. The new concept which we introduce here is named as Programmed Packaging, which is composed of the strategy to targeting, molecular design of functional devices to overcome barriers, and assembly technique to package them into a nanoparticle. In this article, we explain this concept and provide newly developed gene delivery systems based on this concept. Nihon rinsho. Japanese journal of clinical medicine 0047-1852 64 2 258 262 20060200 16454179 read0187881/published_papers/26076395 Atsushi Yamashita Hak Soo Choi Tooru Ooya Nobuhiko Yui Hidetaka Akita Kentaro Kogure Hideyoshi Harashima Improved cell viability of linear polyethylenimine through gamma-cyclodextrin inclusion for effective gene delivery. A polypseudorotaxane consisting of a linear polyethylenimine with Mn of 22,000 (LPEI22k) and gamma-cyclodextrins (gamma-CDs; LPEI22k/gamma-CD) has been examined as a gene carrier. The polyplex formation with luciferase-encoding plasmid DNA (pDNA), intracellular trafficking of polyplex, cytotoxicity, and transfection efficiency were evaluated by various characteristic methods. LPEI22k/gamma-CD formed a pDNA polyplex at higher N/P ratios than LPEI22k; this suggests that the gamma-CD threading sterically interfered with the polyplex formation. In addition, the zeta potentials of the polyplex significantly decreased due to the reduction in charge density of LPEI22k caused by gamma-CD threading. The cellular uptake of pDNA in the LPEI22k/gamma-CD polyplex was enhanced by free gamma-CDs released from the polyplex that might accelerate the cellular uptake through enhanced membrane affinity. LPEI22k/gamma-CD significantly increased cell viability even at high N/P ratios, and the polyplex showed high transfection efficacy. The low cytotoxicity and high gene expression of LPEI22k/gamma-CD are advantageous to polyplex administration in vivo. Chembiochem 1439-4227 7 2 297 302 20060200 10.1002/cbic.200500348 16408311 read0187881/published_papers/47666697 Kenichi Niikura Takashi Nishio Yasutaka Matsuo Hidetaka Akita Kentaro Kogure Hideyoshi Harashima Kuniharu Ijiro Sugar-displaying quantum dots for cellular stress sensing Polymer Preprints, Japan 55 2 5451 5452 20060000 2-s2.0-33846127157 read0187881/published_papers/47652570 Takashi Nishio Kenichi Niikura Yasutaka Matsuo Hidetaka Akita Kentaro Kogure Hideyoshi Harashima Kuniharu Ijiro Synthesis of sugar-displaying quantum dots and their intracellular dynamics Polymer Preprints, Japan 55 1 2145 null 20060000 2-s2.0-33749827431 read0187881/published_papers/38166505 Yamashita A Ooya T Kanda D Choi H.S Yui N Maruyama A Akita H Kogure K Harashima H Effects of supramolecular structure of cationic-polyrotaxanes on pDNA compaction Polymer Preprints, Japan 55 1 null null 20060000 2-s2.0-33749821511 read0187881/published_papers/25294796 H. Akita M. Tanimoto T. Masuda K. Kogure S. Hama K. Ninomiya S. Futaki H. Harashima Evaluation of the nuclear delivery and intra-nuclear transcription of plasmid DNA condensed with μ (mu) and NLS-μ by cytoplasmic and nuclear microinjection: A comparative study with poly-L-lysine Journal of Gene Medicine 8 2 198 206 20060000 10.1002/jgm.839 read0187881/published_papers/25173185 Kanda D Yamashita A Katoono R Ooya T Yui N Maruyama A Akita H Kogure K Harashima H In vitro evaluation of cationic polyrotaxanes as gene carrier Polymer Preprints, Japan 55 2 5367 5368 20060000 2-s2.0-33846129461 read0187881/published_papers/25173177 Ooya T Choi H.S Yamashita A Yui N Sugaya Y Kano A Maruyama A Akita H Ito R Kogure K Harashima H Biocleavable polyrotaxane-plasmid DNA polyplex for enhanced gene delivery Journal of the American Chemical Society 0002-7863 128 12 3852 3853 20060000 10.1021/ja055868+ 16551060 2-s2.0-33645460372 read0187881/published_papers/13929689 K Kogure Kentaro Kogure S Manabe Sachie Manabe Suzuki, I Ichiro Suzuki A Tokumura Akira Tokumura K Fukuzawa Kenji Fukuzawa Cytotoxicity of alpha-tocopheryl succinate, malonate and oxalate in normal and cancer cells in vitro and their anti-cancer effects on mouse melanoma in vivo Cytotoxicity of α-tocopheryl succinate, malonate and oxalate in normal and cancer cells in vitro and their anti-cancer effects on mouse melanoma in vivo JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY Journal of Nutritional Science and Vitaminology 0301-4800 51 6 392 397 20051200 10.3177/jnsv.51.392 16521697 read0187881/published_papers/26076396 Hak Soo Choi Atsushi Yamashita Tooru Ooya Nobuhiko Yui Hidetaka Akita Kentaro Kogure Rie Ito Hideyoshi Harashima Sunflower-shaped cyclodextrin-conjugated poly(epsilon-lysine) polyplex as a controlled intracellular trafficking device. Chembiochem 1439-4227 6 11 1986 1990 20051100 10.1002/cbic.200500242 16222730 read0187881/published_papers/28701737 K Sasaki K Kogure S Chaki Y Kihira M Ueno H Harashima Construction of a multifunctional envelope-type nano device by a SUV*-fusion method INTERNATIONAL JOURNAL OF PHARMACEUTICS 0378-5173 296 1-2 142 150 20050500 10.1016/j.ijpharm.2005.02.020 read0187881/published_papers/26614788 Fukuzawa K Saitoh Y Akai K Kogure K Ueno S Tokumura A Otagiri M Shibata A Antioxidant effect of bovine serum albumin on membrane lipid peroxidation induced by iron chelate and superoxide. Biochimica et biophysica acta 0006-3002 1668 1 145 155 20050200 10.1016/j.bbamem.2004.12.006 15670740 read0187881/published_papers/13929690 K Fukuzawa Y Saitoh K Akai K Kogure S Ueno A Tokumura M Otagiri A Shibata Antioxidant effect of bovine serum albumin on membrane lipid peroxidation induced by iron chelate and superoxide BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 0006-3002 1668 1 145 155 20050200 10.1016/j.bbamem.2004.12.006 15670740 read0187881/published_papers/29875314 Hidetaka Akita Ikramy A. Khalil Kentaro Kogure Hideyoshi Harashima Pharmacokinetics of gene delivery in cells Springer-Verlag Tokyo Non-viral Gene Therapy: Gene Design and Delivery 135 154 20050000 10.1007/4-431-27879-6_11 2-s2.0-84876848321 read0187881/published_papers/28715663 IA Khalil K Kogure S Futaki H Harashima Octaarginine-mediated stimulation of clathrin-independent endocytic uptake leading to enhanced gene expression through avoiding lysosomal degradation YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 0031-6903 125 94 95 20050000 read0187881/published_papers/25849413 Moriguchi R Kogure K Akita H Futaki S Miyagishi M Taira K Harashima H A multifunctional envelope-type nano device for novel gene delivery of siRNA plasmids International Journal of Pharmaceutics 0378-5173 301 1-2 277 285 20050000 10.1016/j.ijpharm.2005.05.021 16019173 read0187881/published_papers/24836786 Shiroh Futaki Yumi Masui Ikuhiko Nakase Yukio Sugiura Takashi Nakamura Kentaro Kogure Hideyoshi Harashima Unique features of a pH-sensitive fusogenic peptide that improves the transfection efficiency of cationic liposomes Harashima 7 1450 1458 20050000 10.1002/jgm.796 read0187881/published_papers/13929691 K Kogure Yamauchi, I A Tokumura K Kondou N Tanaka Y Takaishi K Fukuzawa Novel antioxidants isolated from plants of the genera Ferula, Inula, Prangos and Rheum collected in Uzbekistan PHYTOMEDICINE 0944-7113 11 7-8 645 651 20041100 10.1016/j.phymed.2003.09.004 15636179 read0187881/published_papers/13929692 K Akai K Tsuchiya A Tokumura K Kogure S Ueno A Shibata T Tamaki K Fukuzawa Ability of ferric nitrilotriacetate complex with three pH-dependent conformations to induce lipid peroxidation FREE RADICAL RESEARCH 1071-5762 38 9 951 962 20040900 10.1080/1071576042000261945 15621713 read0187881/published_papers/25294792 K. Kogure R. Moriguchi K. Sasaki M. Ueno S. Futaki H. Harashima Development of a non-viral multifunctional envelope-type nano device by a novel lipid film hydration method Journal of Controlled Release 98 2 317 323 20040811 10.1016/j.jconrel.2004.04.024 read0187881/published_papers/13929693 M Okasaka Y Takaishi K Kogure K Fukuzawa H Shibata T Higuti G Honda M Ito OK Kodzhimatov O Ashurmetov New stilbene derivatives from Calligonum leucocladum JOURNAL OF NATURAL PRODUCTS 0163-3864 67 6 1044 1046 20040600 10.1021/np0304823 15217293 read0187881/published_papers/13929694 K Kogure Kentaro Kogure S Hama Susumu Hama M Kisaki Mayumi Kisaki H Takemasa Hideaki Takemasa A Tokumura Akira Tokumura Suzuki, I Ichiro Suzuki K Fukuzawa Kenji Fukuzawa Structural characteristic of terminal dicarboxylic moiety required for apoptogenic activity of alpha-tocopheryl esters Structural characteristic of terminal dicarboxyric moiety required for apoptogenic activity of α-Tocopheryl esters. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS Biochimica et Biophysica Acta (BBA) - General Subjects 0304-4165 1672 2 93 99 20040500 10.1016/j.bbagen.2004.03.001 15110091 read0187881/published_papers/47540970 K. Fukuzawa K. Kogure M. Morita S. Hama S. Manabe A. Tokumura Review: Enhancement of nitric oxide and superoxide generations by alpha-tocopheryl succinate and its apoptotic and anticancer effects Biokhimiya 0320-9725 69 1 64 73 20040000 2-s2.0-1942489375 read0187881/published_papers/28528332 LA Khalil K Kogure S Futaki H Harashima Factors affecting membrane transport of octaarginine-modified liposomes for gene delivery YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 0031-6903 124 113 116 20040000 read0187881/published_papers/13929704 真鍋 幸恵 Kentaro Kogure 小暮 健太朗 森田 健之 Akira Tokumura 村 彰 Kenji Fukuzawa 福澤 健治 抗ガン作用の増強を志向したビタミンEコハク酸ベシクル ビタミンE研究の進歩? 45 49 20040000 read0187881/published_papers/13929703 Kenji Fukuzawa 福澤 健治 Kentaro Kogure 小暮 健太朗 中島 佐和 土江 明子 Akira Tokumura 村 彰 リン脂質酸化二次作物による血管平滑筋細胞のアポトーシス誘導とビタミンEによる抑制作用 ビタミンE研究の進歩? 115 127 20040000 read0187881/published_papers/13929696 K Kogure S Nakashima A Tsuchie A Tokumura K Fukuzawa Temporary membrane distortion of vascular smooth muscle cells is responsible for their apoptosis induced by platelet-activating factor-like oxidized phospholipids and their degradation product, lysophosphatidylcholine CHEMISTRY AND PHYSICS OF LIPIDS 0009-3084 126 1 29 38 20031100 10.1016/S0009-3084(03)00091-4 14580708 read0187881/published_papers/13929695 A Suga Y Takaishi S Goto T Munakata Yamauchi, I K Kogure Two lignan dimers from bamboo stems (Phyllostachys edulis) PHYTOCHEMISTRY 0031-9422 64 5 991 996 20031100 10.1016/50031-9422(03)00422-9 14561516 read0187881/published_papers/13929697 K Kogure S Hama S Goto T Munakata A Tokumura K Fukuzawa alpha-tocopheryl succinate activates protein kinase C in cellular and cell-free systems JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY 0301-4800 49 5 310 314 20031000 10.3177/jnsv.49.310 14703304 read0187881/published_papers/28553707 T Ochi Y Takaishi K Kogure Yamauti, I Antioxidant activity of a new capsaicin derivative from capsicum annuum JOURNAL OF NATURAL PRODUCTS 0163-3864 66 8 1094 1096 20030800 10.1021/np020465y read0187881/published_papers/13929705 Akira Tokumura b ێR Sj N, V Kentaro Kogure c F, _ G Kenji Fukuzawa 正常および異常妊娠時のヒト血清リゾホスホリパーゼD活性 Proceedings of the Japanese Conference on the Biochemistry of Lipids 脂質生化学研究 0285-1520 45 0 95 97 20030620 read0187881/published_papers/27848257 Kogure K Tsuchiya K Abe K Akasu M Tamaki T Fukuzawa K Terada H Direct radical scavenging by the bisbenzylisoquinoline alkaloid cepharanthine. Biochimica et biophysica acta 0006-3002 1622 1 1 5 20030600 12829254 read0187881/published_papers/13929698 K Kogure K Tsuchiya K Abe M Akasu T Tamaki K Fukuzawa H Terada Direct radical scavenging by the bisbenzylisoquinoline alkaloid cepharanthine BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 0304-4165 1622 1 1 5 20030600 10.1016/S0304-4165(03)00095-3 12829254 read0187881/published_papers/29086521 S Goto K Kogure K Abe Y Kimata K Kitahama E Yamashita H Terada Efficient radical trapping at the surface and inside the phospholipid membrane is responsible for highly potent antiperoxidative activity of the carotenoid astaxanthin BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 0005-2736 1512 2 251 258 20010600 read0187881/published_papers/13929707 Akira Tokumura xi q J R N, V Kentaro Kogure Kenji Fukuzawa モルモット腹腔洗浄液のリゾホスホリパーゼD活性 Proceedings of the Japanese Conference on the Biochemistry of Lipids 脂質生化学研究 0285-1520 43 82 85 20010510 read0187881/published_papers/13929699 K Fukuzawa A Tokumura K Kogure M Iemura N Gondoh M Fujii S Ueno A Shibata A comparative study of the ability of ferric nitriloacetate and other iron chelators to assist membrane lipid peroxidation by superoxide radicals CHEMISTRY AND PHYSICS OF LIPIDS 0009-3084 110 1 69 84 20010300 10.1016/S0009-3084(01)00127-X 11245836 read0187881/published_papers/29645414 Kenji Fukuzawa Akira Tokumura Kentaro Kogure Masahito Iemura Naoto Gondoh Masanobu Fujii Satoru Ueno Akira Shibata Erratum: To "A comparative study of the ability of ferric nitrilotriacetate and other iron chelators to assist membrane lipid peroxidation by superoxide radicals" (Chemistry and Physics of Lipids (2001) 110 (69-84) PII: S000930840100127X) Chemistry and Physics of Lipids 0009-3084 111 2 195 null 20010000 10.1016/S0009-3084(01)00159-1 2-s2.0-0034922322 read0187881/published_papers/13929708 Kenji Fukuzawa Kentaro Kogure M Morita S Hama S Nakashima Akira Tokumura Enhancement by α-tocopherol hemisuccinate of nitric oxide production induced by lipopolysaccharide and interferon-γ through up-regulation of protein kinase C in rat vascular smooth muscle cells Proc.Of the second China-Japan international Conference on Vitamins 262 267 20010000 read0187881/published_papers/13929700 T Chikawa T Ikata S Katoh Y Hamada K Kogure K Fukuzawa Preventive effects of lecithinized superoxide dismutase and methylprednisolone on spinal cord injury in rats: Transcriptional regulation of inflammatory and neurotrophic genes JOURNAL OF NEUROTRAUMA 0897-7151 18 1 93 103 20010100 10.1089/089771501750055802 11200253 read0187881/published_papers/13929709 Akira Tokumura J R xi q N, V Kentaro Kogure k ^, c F Kenji Fukuzawa 血清リゾホスホリパーゼDによるリゾホスファチジン酸産生の病態下での変動 Proceedings of the Japanese Conference on the Biochemistry of Lipids 脂質生化学研究 0285-1520 42 41 44 20000000 read0187881/published_papers/13929710 Akira Tokumura l N Kentaro Kogure V Kenji Fukuzawa c 高度不飽和脂肪酸を有するリゾホスファチジン酸:血漿中での生成と血小板凝集活性 Proceedings of the Japanese Conference on the Biochemistry of Lipids 脂質生化学研究 0285-1520 41 193 195 19990000 read0187881/published_papers/13929701 J ICHIHARA Y SHINOHARA K KOGURE H TERADA NUCLEOTIDE-SEQUENCE OF THE 5'-FLANKING REGION OF THE RAT TYPE-II HEXOKINASE GENE BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 0167-4781 1260 3 365 368 19950200 10.1016/0167-4781(94)00226-S 7873617 read0187881/published_papers/13929702 Y SHINOHARA K YAMAMOTO K KOGURE J ICHIHARA H TERADA STEADY-STATE TRANSCRIPT LEVELS OF THE TYPE-II HEXOKINASE AND TYPE-1 GLUCOSE-TRANSPORTER IN HUMAN TUMOR-CELL LINES CANCER LETTERS 0304-3835 82 1 27 32 19940700 read0187881/misc/43989682 高橋 尚子 清水 良多 森戸 克弥 東 桃代 下澤 伸行 福田 達也 小暮 健太朗 田中 保 液体クロマトグラフィー/タンデム質量分析による副腎白質ジストロフィー患者の血漿中セラミド分子種及び濃度の解析 (公社)日本生化学会 日本生化学会大会プログラム・講演要旨集 92 [2T15a 05] 20190900 read0187881/misc/43989679 森戸 克弥 島田 明奈 宮崎 徹 清水 良多 高橋 尚子 下澤 伸行 東 桃代 福田 達也 小暮 健太朗 田中 保 ヒト血漿の主要なセラミド及びセラミド1-リン酸分子種の動物細胞への取り込みと作用 (公社)日本生化学会 日本生化学会大会プログラム・講演要旨集 92 [2T15a 04] 20190900 read0187881/misc/43989668 田中 保 森戸 克弥 YESMIN Hasi Rumana Yesmin Hasi Rumana 林 順司 川上 竜巳 金丸 芳 若山 睦 近藤 千恵子 福田 達也 小暮 健太朗 A convenient method for determination of creamed in foodstuffs 食品素材に含まれるセラミドの簡便な定量方法 日本脂質栄養学会 脂質栄養学 1883-2237 28 2 125 125 20190900 read0187881/misc/43989692 森戸 克弥 島田 明奈 宮崎 徹 清水 良多 高橋 尚子 東 桃代 下澤 伸行 西岡 安彦 福田 達也 小暮 健太朗 田中 保 ヒト血漿中セラミド及びセラミド1-リン酸の分子種組成と動物細胞への取り込みと作用 日本脂質生化学会 脂質生化学研究 0285-1520 61 98 99 20190600 read0187881/misc/47573518 福田達也 小暮健太朗 Development of Leukocyte-mimetic Nanoparticles to Overcome the Blood-Brain Barrier in the Region of Ischemic Stroke 脳梗塞部位の血液脳関門突破を目指した 白血球模倣ナノ粒子の開発 膜 0385-1036 44 5 null null 20190000 read0187881/misc/43994423 森戸克弥 島田明奈 宮崎徹 清水良多 高橋尚子 下澤伸行 東桃代 小山壱也 西岡安彦 福田達也 小暮健太朗 田中保 ヒト血漿中セラミド及びセラミド1-リン酸の分子種組成と動物細胞へ作用 日本農芸化学会大会講演要旨集(Web) 2186-7976 2019 null null 20190000 read0187881/misc/43989696 森戸 克弥 清水 良多 高橋 尚子 下澤 伸行 東 桃代 河野 弘 西岡 安彦 福田 達也 小暮 健太朗 田中 保 ヒト血漿中セラミドおよびセラミド1-リン酸の分子種組成と細胞への取り込み (公社)日本生化学会 日本生化学会大会プログラム・講演要旨集 91 [3P 063] 20180900 read0187881/misc/43989688 田中 保 森戸 克弥 清水 良多 北村 苗穂子 朴 時範 岸野 重信 小川 順 福田 達也 小暮 健太朗 腸内細菌が産生するヒドロキシ脂肪酸の動物細胞における代謝 日本脂質栄養学会 脂質栄養学 1883-2237 27 2 141 141 20180800 read0187881/misc/25258405 小暮健太朗 石川みすず 平井将太 浜進 吉田達貞 高橋侑 細井信造 福田達也 田中保 α‐トコトリエノールとアスタキサンチンの相乗的抗酸化効果 ビタミン 0006-386X 92 5/6 284-285 null 20180625 read0187881/misc/43989700 宮崎 徹 島田 明奈 高橋 尚子 Rahman Md.Motiur 清水 良多 辻 和樹 森戸 克弥 山下 量平 佐野 茂樹 中尾 允泰 福田 達也 小暮 健太朗 田中 保 外因的に加えた極長鎖脂肪酸および極長鎖脂肪酸含有セラミドのアポトーシスへの影響 日本脂質生化学会 脂質生化学研究 0285-1520 60 237 239 20180500 read0187881/misc/43994444 田中保 宮城諒 辻和樹 藤原美奈 森戸克弥 石川寿樹 今井博之 川合真紀 福田達也 小暮健太朗 植物に見出されたグリコシルイノシトールホスホセラミド特異的ホスホリパーゼDの性質 日本農芸化学会中四国支部講演会講演要旨集(Web) 51st null null 20180000 read0187881/misc/43994440 宮城諒 辻和樹 藤原美奈 森戸克弥 石川寿樹 今井博之 川合真紀 福田達也 小暮健太朗 田中保 植物に見出されたグリコシルイノシトールホスホセラミド特異的ホスホリパーゼDの性質 日本生化学会大会(Web) 91st null null 20180000 read0187881/misc/43994428 森戸克弥 清水良多 北村苗穂子 PARK Si-Bum 岸野重信 小川順 福田達也 小暮健太朗 田中保 乳酸菌が産生する希少脂肪酸のペルオキシソームにおける代謝 日本農芸化学会大会講演要旨集(Web) 2186-7976 2018 null null 20180000 read0187881/misc/10972254 小暮 健太朗 【核酸医薬品の進歩と課題】 イオントフォレシスによる核酸医薬の展開 微弱電流による組織細胞生理の制御 医歯薬出版(株) 医学のあゆみ 0039-2359 262 2 153 156 20170700 read0187881/misc/25258408 小暮健太朗 石川みすず 平井将太 浜進 細井信造 吉田達貞 山下栄次 高橋侑 ビタミンEとの相乗的抗酸化効果におけるアスタキサンチン立体構造の影響 日本酸化ストレス学会学術集会プログラム・抄録集 70th 113 null 20170628 read0187881/misc/10814280 Kentaro Kogure 小暮 健太朗 イオントフォレシスによる核酸医薬の展開-微弱電流による組織細胞生理の制御 Journal of Clinical and Experimental Medicine 医学のあゆみ 0039-2359 262 153 156 20170600 read0187881/misc/25258410 平井将太 石川みすず 渋谷菜摘 浜進 細井信造 高橋侑 山下栄次 小暮健太朗 小暮健太朗 トコトリエノールとアスタキサンチンの相乗的な抗酸化活性向上における至適比率の検討 ビタミン 0006-386X 91 4 294 null 20170425 read0187881/misc/10814281 Kentaro Kogure 小暮 健太朗 アスタキサンチンのヘマトコッカス培養による生産と機能性研究 アグリバイオ 1 9 13 20170400 read0187881/misc/10972255 小暮 健太朗 濱 進 ビタミンのケミカルバイオロジー研究 ビタミンEコハク酸の多彩な生理作用と薬学への展開 (公社)日本ビタミン学会 ビタミン 0006-386X 91 3 182 187 20170300 read0187881/misc/25258412 小暮健太朗 亀崎ちひろ 中島愛美 石橋博 浜進 細井信造 山下栄次 アスタキサンチンとビタミンE共存による抗酸化活性の向上 日本酸化ストレス学会学術集会プログラム・抄録集 69th 151 null 20160830 read0187881/misc/25258414 小暮健太朗 亀崎ちひろ 中島愛美 石橋博 浜進 細井信造 山下栄次 ビタミンEとアスタキサンチン共存による抗酸化作用の向上効果 ビタミン 0006-386X 90 4 186 null 20160425 read0187881/misc/25274140 Hasan, M Nishimoto, A Ohgita, T Hama, S Kashida, H Asanuma, H Kogure, K Faint electric treatment-induced rapid endosomal escape for efficient delivery of functional macromolecules into the cytoplasm J. Control. Release 228 20-25 null 20160000 read0187881/misc/10814283 Kentaro Kogure 小暮 健太朗 ナノメディシン送達のための微弱電流による組織細胞生理の制御 化学工業 67 14 20 20160000 read0187881/misc/35329187 濱 進 大石 利一 福澤 健治 小暮 健太朗 1-I-6 トコフェロールコハク酸ナノベシクルの細胞内動態制御による抗腫瘍効果の増強(一般演題要旨,ビタミン・バイオファクター研究のさらなる魅力∼大和まほろぱからの発信∼,第67回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 89 4 211 211 20150425 read0187881/misc/35329170 西 貴之 濱 進 桐村 直子 鈴木 智子 板倉 祥子 福澤 健治 小暮 健太朗 1-I-5 腹膜播種治療を目指した量子ドット封入トコフェロールコハク酸ナノベシクルの開発(一般演題要旨,ビタミン・バイオファクター研究のさらなる魅力∼大和まほろぱからの発信∼,第67回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 89 4 211 211 20150425 read0187881/misc/35329099 桐村 直子 濱 進 北尾 哲也 福澤 健治 小暮 健太朗 1-I-4 リウマチ患者由来滑膜細胞におけるリウマチ進展遺伝子発現に対するトコフェロールリン酸の影響(一般演題要旨,ビタミン・バイオファクター研究のさらなる魅力∼大和まほろぱからの発信∼,第67回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 89 4 210 210 20150425 read0187881/misc/10972256 小暮 健太朗 濱 進 梶本 和昭 【新たな医療のディメンジョンを模索するオレオナノサイエンス】 微弱電流による高分子・ナノ粒子の皮内送達 (公社)日本油化学会 オレオサイエンス 1345-8949 15 3 131 137 20150300 10.5650/oleoscience.15.131 read0187881/misc/37282373 濱 進 小暮 健太朗 Development of Effective DDS Having Appropriate Structural Properties Responsive to Tumor Environment 標的に応じた構造特性を有するがん治療DDS (新春特集 薬剤学研究が織りなすドラックデリバリー技術とフォーミュレーションデザイン) シーエムシー出版 ファインケミカル : 調査・資料・報道・抄録 0913-6150 44 1 29 37 20150100 read0187881/misc/25339493 Hama S Nakamura I Nishimoto A Nishi T Itakura S Kogure K Lipocalin2 stabilizes hypoxia inducible factor-1α through the iron delivery into normoxic cancer cells Molecular Cancer Therapeutics 14 A202 A202 20150000 read0187881/misc/25339488 小暮健太朗 濱 進 梶本和昭 イオントフォレシスによる機能成分の皮内浸透メカニズム Cosmetic Stage 9 1 6 20150000 read0187881/misc/10972257 濱 進 小暮 健太朗 【特殊な膜を用いたナノ粒子の開発とDDSへの応用】 腫瘍環境応答性素子と脂質膜との相互作用を利用したDDSの創製 日本膜学会 膜 0385-1036 39 5 277 282 20140900 10.5360/membrane.39.277 read0187881/misc/28040499 Kentaro Kogure Susumu Hama Masahiko Yamamoto Kiyoshi Kanamura Kaoru Kigasawa Kazuaki Kajimoto NONINVASIVE AND EFFICIENT DELIVERY OF MACROMOLECULAR MEDICINE VIA IONTOPHORESIS JOURNAL OF GENE MEDICINE 1521-2254 16 7-8 213 213 20140700 read0187881/misc/35219479 濱 進 下井 雄太 桐村 直子 北尾 哲也 福澤 健治 小暮 健太朗 2-I-17 ビタミンEによるリウマチ進展遺伝子発現抑制を介した抗リウマチ効果(一般演題要旨,第66回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 88 4 235 235 20140425 read0187881/misc/35219454 下井 雄太 濱 進 福澤 健治 小暮 健太朗 2-I-16 作用メカニズム解析情報に基づくトコフェロールコハク酸の抗癌効果の増強(一般演題要旨,第66回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 88 4 235 235 20140425 read0187881/misc/28089558 Kentaro Kogure Nanoparticles for Cancer Therapy Foreword BIOLOGICAL & PHARMACEUTICAL BULLETIN 0918-6158 37 2 195 195 20140200 read0187881/misc/25696787 池田義人 小暮健太朗 脂肪細胞に対するsiRNA導入によるアディポカイン``レジスチン''の機能解析とメタボリックシンドローム治療への展望 Antisense 18 1 21 34 20140000 read0187881/misc/25339494 濱 進 下井雄太 福澤健治 小暮健太朗 トコフェロールコハク酸誘導アポトーシスにおけるオートファジーの関与 ビタミンE研究の進歩XVI 50 55 20140000 read0187881/misc/25339490 濱 進 福澤健治 小暮健太朗 ビタミンE誘導体によるがん細胞特異的作用とがん治療への展開 ビタミン 88 82 90 20140000 read0187881/misc/35521433 KOGURE Kentaro 小暮 健太朗 To Young Scientists and Students Who Will Lead the Next Generation 次代を担う若い皆さんへ 薬剤学 = The archives of practical pharmacy 0372-7629 73 3 143 143 20130501 read0187881/misc/25341137 松尾保隆 加地範匡 畠山浩人 渡慶次学 小暮健太朗 馬場嘉信 原島秀吉 Nano Packagingが拓く次世代核酸医療 表面 51 227-240 null 20130500 read0187881/misc/35237025 岡村 有里子 濱 進 福澤 健治 小暮 健太朗 2-IV-17 トコフェロールコハク酸によるangiopoietin-2抑制を介した血管の構造安定化作用(一般演題要旨,第65回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 87 4 257 257 20130425 read0187881/misc/35237010 下井 雄太 濱 進 中村 伊吹 大石 利一 福田 友紀 岡村 有里子 土谷 博之 福澤 健治 小暮 健太朗 2-IV-16 活性酸素を介したトコフェロールコハク酸誘導オートファジーによるアポトーシス耐性の獲得(一般演題要旨,第65回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 87 4 256 256 20130425 read0187881/misc/10972258 濱 進 小暮 健太朗 【ここまで広がるドラッグ徐放技術の最前線-古くて新しいドラッグデリバリーシステム(DDS)-】 (第4章)徐放技術と外部刺激との組み合わせ pH応答性の薬物キャリア (株)メディカルドゥ 遺伝子医学MOOK 1349-2527 357 361 20130300 read0187881/misc/26167756 加地 範匡 北添 雄眞 重中 大輔 小暮 健太朗 秋田 英万 原島 秀吉 渡慶次 学 馬場 嘉信 マイクロ流体チップによる多機能性エンベロープ型ナノ構造体の作製 日本DDS学会 日本DDS学会学術集会プログラム予稿集 28 182 182 20120600 read0187881/misc/35408673 福田 友紀 濱 進 内海 達 福澤 健治 小暮 健太朗 1-II-12 抗腫瘍活性の高いトコフェロールコハク酸ナノ粒子の作用機構の解析(一般演題要旨,日本ビタミン学会第64回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 86 4 238 238 20120425 read0187881/misc/35408606 北尾 哲也 濱 進 福澤 健治 小暮 健太朗 1-II-9 関節リウマチ進展因子に対するトコフェロール誘導体の影響(一般演題要旨,日本ビタミン学会第64回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 86 4 237 237 20120425 read0187881/misc/35408604 岡村 有里子 濱 進 土谷 博之 福澤 健治 小暮 健太朗 1-II-11 脈管新生因子angiopoietin-2に対するトコフェロールコハク酸の抑制効果(一般演題要旨,日本ビタミン学会第64回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 86 4 238 238 20120425 read0187881/misc/35408370 河原 亜里紗 池田 義人 濱 進 土谷 博之 小暮 健太朗 1-II-6 α-トコフェロールの脂肪細胞におけるPPARγ非依存的脂肪蓄積促進作用(一般演題要旨,日本ビタミン学会第64回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 86 4 235 235 20120425 read0187881/misc/35406755 池田 弥子 高橋 加奈子 濱 進 山下 栄次 小暮 健太朗 2-V-13 活性酸素による皮膚細胞傷害に対するアスタキサンチンの効果(一般演題要旨,日本ビタミン学会第64回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 86 4 287 287 20120425 read0187881/misc/25490294 梶本和昭 兵藤守 小暮健太朗 原島秀吉 DDS技術による遺伝子・核酸医薬の将来 月刊ファインケミカル 特集 先端医療実用化のための最新DDS技術の動向 41 4 31 36 20120000 read0187881/misc/25339496 濱 進 内海 達 福田友紀 中山佳代子 岡村有里子 土谷博之 福澤健治 小暮健太朗 トコフェロールコハク酸ナノベシクルの抗腫瘍効果増強メカニズムの解析 ビタミンE研究の進歩XV 128 133 20120000 read0187881/misc/25237115 濱 進 中村 伊吹 大石 利一 土谷 博之 桑原 義和 福本 学 福澤 健治 小暮 健太朗 放射線耐性細胞におけるトコフェロールコハク酸の細胞死誘導効果(一般演題要旨,日本ビタミン学会第64回大会講演要旨) 86 4 237 null 20120000 10.20632/vso.86.4_237_2 read0187881/misc/10972259 小暮 健太朗 高分子医薬の新たな送達ルートとしての皮内送達 (公社)日本薬学会 ファルマシア 0014-8601 47 12 1121 1125 20111200 read0187881/misc/10972260 小暮 健太朗 濱 進 ナノ粒子表面物性に基づいたDDS開発 腫瘍環境応答性の表面物性を有するナノキャリアーの開発 For achievement of efficient antitumor therapy, we developed novel DDS nano-carriers, which have specific surface properties suitable for <i>in vivo</i> delivery to tumor. In order to avoid interaction with biological components, such as serum protein and reticuloendothelial system, we focused attention on the carriers having negatively charged surface. At first, we constructed negatively charged DDS carrier by tocopheryl succinate (TS), which is known as an anti-tumor vitamin E derivative. The DDS carrier consisting of TS showed potent anti-tumor activity. However, cellular uptake was not enough because of its negatively charged surface. Then, we constructed a novel DDS nano-carrier, which can change surface charge dependent on environmental pH. The novel pH sensitive DDS nano-carrier showed pH-dependent cellular uptake and functionality. Moreover, the nano-carrier accumulated in the tumor like PEG-liposome, although the carrier has no PEG modification to maintain blood circulation time. Consequently, we developed novel DDS nano-carriers having surface functionality responsive to environment in tumor. 日本膜学会 膜 0385-1036 36 4 171 176 20110700 10.5360/membrane.36.171 read0187881/misc/10972261 小暮 健太朗 濱 進 梶本 和昭 気賀澤 郁 【ペプチド・タンパク性医薬品の新規DDS製剤の開発と応用】 (第4章)各種粘膜吸収経路を利用したペプチド・タンパク性医薬品の消化管ならびに経粘膜吸収改善 イオントフォレシスを用いたペプチド性医薬品の経皮送達 (株)メディカルドゥ 遺伝子医学MOOK 1349-2527 ·DDS 209 213 20110600 read0187881/misc/38018540 高橋 加奈子 池田 弥子 堀田 明里 濱 進 山下 栄次 小暮 健太朗 2-III-24 紫外線による皮膚の遺伝子発現変動とアスタキサンチンの効果(一般演題要旨,日本ビタミン学会第63回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 85 4 244 244 20110425 read0187881/misc/38018488 植西 左千子 濱 進 山下 栄次 小暮 健太朗 2-III-22 ヒドロキシラジカルの細胞傷害に対するアスタキサンチン含有リポソームの保護効果(一般演題要旨,日本ビタミン学会第63回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 85 4 243 243 20110425 read0187881/misc/38018482 稲井 優子 濱 進 山下 栄次 小暮 健太朗 2-III-23 アスタキサンチンの抗酸化活性に対する脂質膜組成の影響(一般演題要旨,日本ビタミン学会第63回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 85 4 244 244 20110425 read0187881/misc/38018452 濱 進 内海 達 福田 友紀 土谷 博之 福澤 健治 小暮 健太朗 1-I-1 核酸医薬を封入したトコフェロールコハク酸ナノ粒子の機能性評価(一般演題要旨,日本ビタミン学会第63回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 85 4 207 207 20110425 read0187881/misc/38018412 高木 愛未 濱 進 嶌岡 真理 岡村 有里子 土谷 博之 福澤 健治 小暮 健太朗 1-I-2 トコフェロールコハク酸による腫瘍血管新生抑制機構の解析(一般演題要旨,日本ビタミン学会第63回大会講演要旨) 日本ビタミン学会 ビタミン 0006-386X 85 4 207 207 20110425 read0187881/misc/26553757 土谷博之 池田義人 米田紀子 大城祥子 坂部友彦 小暮健太朗 汐田剛史 レチノイン酸によるレプチン依存的インスリン抵抗性改善作用 ビタミン 0006-386X 85 4 216 null 20110425 read0187881/misc/25339491 小暮健太朗 気賀澤 郁 濱 進 梶本和昭 イオントフォレシスによる高分子医薬の皮内送達 薬剤学 71 94 98 20110000 read0187881/misc/10972262 小暮 健太朗 気賀澤 郁 濱 進 梶本 和昭 イオントフォレシスによる高分子の皮膚透過 (一社)日本生物物理学会 生物物理 0582-4052 50 4 188 189 20100700 10.2142/biophys.50.188 read0187881/misc/38139601 小暮 健太朗 原島 秀吉 Drug delivery system via multifunctional envelope-type nano device 多機能性エンベロープ型ナノ構造体によるDDS 広信社 表面 0367-648X 48 5 163 175 20100500 read0187881/misc/37346763 内海 達 濱 進 森本 二郎 奥野 貴士 尾関 哲也 辰巳 泰我 福澤 健治 小暮 健太朗 1-II-6 改良型トコフェロールコハク酸ナノ粒子の抗腫瘍効果(一般演題,日本ビタミン学会第62回大会発表要旨) 日本ビタミン学会 ビタミン 0006-386X 84 4 161 161 20100425 read0187881/misc/37346745 濱 進 高木 愛未 内海 達 伊南 かなえ 池田 義人 福澤 健治 小暮 健太朗 1-II-7 トコフェロールコハク酸の血管新生抑制作用(一般演題,日本ビタミン学会第62回大会発表要旨) 日本ビタミン学会 ビタミン 0006-386X 84 4 162 162 20100425 read0187881/misc/40765515 内海 達 濱 進 森本 二郎 辰巳 泰我 尾関 哲也 福澤 健治 小暮 健太朗 膜物性制御したα-トコフェロール誘導体ナノ粒子の抗腫瘍効果 (公社)日本薬剤学会 薬剤学: 生命とくすり 0372-7629 70 Suppl. 111 111 20100400 read0187881/misc/39412885 北添雄眞 岡本行広 岡本行広 加地範匡 加地範匡 渡慶次学 渡慶次学 小暮健太郎 原島秀吉 馬場嘉信 馬場嘉信 馬場嘉信 馬場嘉信 マイクロ流体デバイスによる遺伝子治療用ナノデバイスの高速作製 日本化学会講演予稿集 0285-7626 90th 3 null null 20100000 read0187881/misc/27419607 K. Kitazoe Y. Okamoto N. Kaji M. Tokeshi K. Kogure H. Harashima Y. Baba A microdevice with chaotic mixer to constract multifunctional envelope-type nanodevice for delivery system 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 2 827 829 20100000 2-s2.0-84884404240 read0187881/misc/29015305 I. A. Khalil K. Kogure M. Yamada H. Harashima OCTAARGININE-MODIFIED MULTIFUNCTIONAL ENVELOPE-TYPE NANOPARTICLES FOR GENE DELIVERY JOURNAL OF GENE MEDICINE 1099-498X 11 12 1171 1171 20091200 read0187881/misc/29013410 Ayman El-Sayed Ikramy A. Khalil Kentaro Kogure Hideyoshi Harashima COMPARATIVE EVALUATION OF OCTAARGININE AND OCTALYSINE MODIFIED NANOPARTICLES FOR INTRACELLULAR DELIVERY OF PLASMID DNA AND SIRNA JOURNAL OF GENE MEDICINE 1099-498X 11 12 1178 1178 20091200 read0187881/misc/37738519 濱 進 内海 達 福澤 健治 小暮 健太朗 2-III-17 抗がん効果向上を目指したトコフェロールコハク酸ナノ粒子の製剤学的工夫(一般研究発表,日本ビタミン学会第61回大会研究発表要旨) 日本ビタミン学会 ビタミン 0006-386X 83 4 216 216 20090425 read0187881/misc/39413016 北添雄眞 加地範匡 加地範匡 岡本行広 岡本行広 渡慶次学 渡慶次学 小暮健太郎 原島秀吉 馬場嘉信 馬場嘉信 馬場嘉信 馬場嘉信 馬場嘉信 Rapid construction of Multifunctional Envelope-type Nanodevice using microfluidic device マイクロ流体デバイスを用いた多機能性エンベロープ型ナノ構造体の高速構築 日本化学会講演予稿集 0285-7626 89th 2 null null 20090000 read0187881/misc/39412890 北添雄眞 加地範匡 加地範匡 岡本行広 岡本行広 渡慶次学 渡慶次学 小暮健太朗 原島秀吉 馬場嘉信 馬場嘉信 馬場嘉信 馬場嘉信 マイクロ流体デバイスを利用した多機能性エンベロープ型ナノ構造体の高速作製 化学とマイクロ・ナノシステム研究会講演要旨集 20th null null 20090000 read0187881/misc/25341000 Katsuma Kitazoe Noritada Kaji Manabu Tokeshi Kentaro Kogure Hideyoshi Harashima Yoshinobu Baba MICRODEVICE TO CONSTRACT MULTIFUNCTIONAL ENVELOPE-TYPE NANODEVICE FOR GENE THERAPY Micro Total Analysis System 2008 1891-1893 null 20081000 read0187881/misc/39168225 畠山 浩人 秋田 英万 小暮 健太朗 Development of multifunctional envelope-type nano device for gene delivery 多機能エンベローブ型ナノ構造体による人工遺伝子デリバリーシステムの創製 (特集 デリバリー技術の最先端) 化学工業社 化学工業 0451-2014 59 4 263 268 20080400 read0187881/misc/24911455 KOGURE Kentaro 小暮 健太朗 AKITA Hidetaka 秋田 英万 HARASHIMA Hideyoshi 原島 秀吉 Development of Various Multifunctional Envelope-type Nano Device MEND Based on Novel Assembly Technologies(Symposium Reviews) 新しいアセンブリー技術による多様な多機能性エンベロープ型ナノ構造体MENDの構築(誌上シンポジウム) Non-viral vectors need to overcome several barriers such as the plasma membrane, the endosomal membrane and the nuclear membrane for efficient gene delivery to the nucleus of target cells. To overcome these obstacles, the delivery system must be equipped with various functional devices. However, it is difficult to package all these needed devices into a single system to exert each of their functions at the appropriate time and at the correct location. Thus, our group proposed a new packaging concept, &quot;Programmed Packaging&quot;. A multifunctional envelope-type nano device (MEND) was developed fo... The Pharmaceutical Society of Japan 公益社団法人日本薬学会 Journal of the Pharmaceutical Society of Japan 藥學雜誌 0031-6903 128 2 219 232 20080201 10.1248/yakushi.128.219 read0187881/misc/29154573 Kentaro Kogure Masahiko Yamamoto Misuzu Watanabe Kiyoshi Kanemura Akiyoshi Saito Hideyoshi Harashima Kazuaki Kajimoto Transdermal delivery of insulin-encapsulated liposomes via iontophoresis YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 0031-6903 128 81 82 20080000 read0187881/misc/29151525 I. A. Khalil K. Kogure M. Yamada H. Harashima Efficient in-vitro and in-vivo gene transfer mediated by octaarginine-modified nanoparticles YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 0031-6903 128 115 116 20080000 read0187881/misc/26160805 YAMADA Yuma AKITA Hidetaka KAMIYA Hiroyuki KOGURE Kentaro YAMAMOTO Takenori SHINOHARA Yasuo YAMASHITA Kikuji KOBAYASHI Hideo KIKUCHI Hiroshi HARASHIMA Hideyoshi MITO-Porter: a liposome-based carrier system for delivery of macromolecules into mitochondria via membrane fusion. Biochimica et Biophysica Acta 0005-2728 1778 2 423 432 20080000 10.1016/j.bbamem.2007.11.002 read0187881/misc/24911457 HATAKEYAMA Hiroto 畠山 浩人 AKITA Hidetaka 秋田 英万 KOGURE Kentaro 小暮 健太朗 HARASHIMA Hideyoshi 原島 秀吉 Development of a Novel Systemic Gene Delivery System for Cancer Therapy with a Tumor-specific Cleavable PEG-lipid(Symposium Review) 腫瘍特異的に分解されるPEG脂質誘導体を用いた全身投与型腫瘍遺伝子デリバリーシステムの開発(誌上シンポジウム) For successful cancer gene therapy via intravenous administration, it is essential to optimize the stability of carriers in the systemic circulation and the cellular association after the accumulation of the carrier in tumor tissue. However, a dilemma exists regarding the use of poly (ethylene glycol) (PEG), which is useful for conferring stability in the systemic circulation, but is undesirable for the cellular uptake and subsequent processes. We report the development of a PEG-peptide-lipid ternary conjugate (PPD). In this strategy, PEG is removed from the carriers via cleavage by a matri... The Pharmaceutical Society of Japan 公益社団法人日本薬学会 Journal of the Pharmaceutical Society of Japan 藥學雜誌 0031-6903 127 10 1549 1556 20071001 10.1248/yakushi.127.1549 read0187881/misc/24911456 HARASHIMA Hideyoshi 原島 秀吉 KOGURE Kentaro 小暮 健太朗 YAMADA Yuma 山田 勇磨 AKITA Hidetaka 秋田 英万 KAMIYA Hiroyuki 紙谷 浩之 Development of Multifunctional Envelope Type Artificial Viral like Gene Delivery System 多機能性エンベロープ型人工遺伝子デリバリーシステムの創製 This review introduces a new concept &quot;Programmed Packaging&quot; to develop a non-viral gene delivery system. Based on this concept, multifunctional envelope type nano devices (MEND) were developed for in vitro, in situ and in vivo conditions. A quantitative study to identify a rate limiting step in intracellular trafficking was also shown between viral and non-viral vectors, which indicated an important role of controlled intranuclear disposition for development a safe and efficient non-viral gene delivery system. This review will provide a future direction of non-viral gene delivery system. The Pharmaceutical Society of Japan 公益社団法人日本薬学会 Journal of the Pharmaceutical Society of Japan 藥學雜誌 0031-6903 127 10 1655 1672 20071001 10.1248/yakushi.127.1655 read0187881/misc/27392036 西尾崇 新倉謙一 松尾保孝 秋田英万 小暮健太朗 原島秀吉 居城邦治 居城邦治 単一細胞のストレスをイメージングできる糖鎖プローブ分子の開発 高分子学会予稿集(CD-ROM) 56 2 Disk1 1Y17 null 20070904 read0187881/misc/10972263 小暮 健太朗 医療薬科学 RNA干渉によって現実化しつつある遺伝子治療 (公社)日本薬学会 ファルマシア 0014-8601 43 9 916 917 20070900 read0187881/misc/27392017 新倉謙一 西尾崇 松尾保孝 秋田英万 小暮健太朗 原島秀吉 居城邦治 居城邦治 細胞のストレスイメージングを可能とする糖鎖プローブの開発 日本糖質学会年会要旨集 27th 79 null 20070710 read0187881/misc/24911458 HARASHIMA Hideyoshi 原島 秀吉 KOGURE Kentaro 小暮 健太朗 AKITA Hidetaka 秋田 英万 YAMADA Yuma 山田 勇磨 KAMIYA Hiroyuki 紙谷 浩之 Development of in vivo gene delivery system by multifunctional envelope-type nano device : Awarded with Nagai Prize in 7th Annual Meeting of the Japan Society of Drug Delivery System 多機能性エンベロープ型ナノ構造体による人工遺伝子デリバリーシステムの創製 : 第7回日本DDS学会永井賞受賞によせて 日本DDS学会 Drug delivery system 0913-5006 22 5 569 577 20070610 read0187881/misc/26295394 山田勇磨 秋田英万 紙谷浩之 小暮健太朗 篠原康雄 原島秀吉 細胞内FRET法を用いたMITO‐Porter(ミトコンドリア膜融性リポソーム)の膜融合能評価 Drug Deliv Syst 0913-5006 22 3 315 null 20070530 read0187881/misc/39349136 小野 耕平 森口 留美子 中村 孝司 小暮 健太朗 原島 秀吉 オクタアルギニン修飾リポソームによるMHCクラスI特異的抗原デリバリーメカニズムの解析 薬剤学 = Journal of Pharmaceutical Science and Technology, Japan 0372-7629 67 293 293 20070510 read0187881/misc/39348753 EL-SAYED Ayman KHALIL Ikramy 小暮 健太朗 原島 秀吉 Stepwise dissection of the Mechanism of Macropinosomal Escape for Octaarginine Modified Nanoparticles 薬剤学 = Journal of Pharmaceutical Science and Technology, Japan 0372-7629 67 361 361 20070510 read0187881/misc/10972264 秋田 英万 小暮 健太朗 原島 秀吉 【細胞内情報デリバリーの新手法】 多機能性エンベロープ型ナノ構造体による遺伝子デリバリー (株)メディカルレビュー社 再生医療 1347-7919 6 2 161,122 167,122 20070500 read0187881/misc/27392005 西尾崇 新倉謙一 松尾保孝 秋田英万 小暮健太朗 原島秀吉 居城邦治 O‐GlcNAc修飾量子ドットを用いた新規細胞ストレス検出法の開発 日本化学会講演予稿集 0285-7626 87th 2 1365 null 20070312 read0187881/misc/26295400 山田勇磨 秋田英万 紙谷浩之 小暮健太朗 篠原康雄 小林英夫 菊池寛 原島秀吉 ミトコンドリア選択的な高分子薬物送達を可能とする薬物送達システムの開発 日本薬学会年会要旨集 0918-9823 127th 1 284 null 20070305 read0187881/misc/24911403 Hidetaka Akita Akita Hidetaka Rie Ito Ito Rie Hiroyuki Kamiya Kamiya Hiroyuki Kentaro Kogure Kogure Kentaro Hideyoshi Harashima Harashima Hideyoshi Cell cycle dependent transcription, a determinant factor of heterogeneity in cationic lipid-mediated transgene expression. BACKGROUND: Heterogeneity of transgene expression, the presence or absence (below the limit of detection) of transgene expression on a cell-by-cell basis, is a severe disadvantage in the use of cationic lipid-mediated gene vectors for gene therapy and experiments in molecular biology. Understandings of intracellular trafficking and the function (transgene expression) of vectors related to cellular physiology are essential in terms of clarifying the mechanism underlying the heterogeneity. METHODS: To distinguish the contribution of nuclear transfer efficiency and subsequent intranuclear transcription efficiency to the overall heterogeneity in transgene expression, a novel imaging system was established for the dual visualization of the nuclear transfer of pDNA and marker gene expression (lacZ) in single cells. RESULTS: The expression of LacZ occurred in only approximately 30% of HeLa cells of the nuclear pDNA-positive cells, indicating that intranuclear transcription efficiency contributed to the heterogeneity. Dual imaging against synchronized cells further revealed that the efficiency of nuclear delivery was comparable irrespective of cell cycle status, which is contrary to the generally accepted hypothesis that nuclear import of pDNA is enhanced during cell division when the nuclear membrane structure is perturbed. The most significant finding in the present study is that nuclear transcription efficiency in terms of the ratio of LacZ-positive cells to nuclear pDNA-positive cells drastically increased in the late S and G2/M phase. CONCLUSIONS: This is the first demonstration to show that cell cycle dependent intranuclear transcription appears to be responsible for the overall heterogeneity of transgene expression. Background: Heterogeneity of transgene expression, the presence or absence (below the limit of detection) of transgene expression on a cell-by-cell basis, is a severe disadvantage in the use of cationic lipid-mediated gene vectors for gene therapy and experiments in molecular biology. Understandings of intracellular trafficking and the function (transgene expression) of vectors related to cellular physiology are essential in terms of clarifying the mechanism underlying the heterogeneity. Methods: To distinguish the contribution of nuclear transfer efficiency and subsequent intranuclear tran... John Wiley & Sons The journal of gene medicine The Journal of Gene Medicine 1099-498X 9 3 197 207 20070300 10.1002/jgm.1010 17351985 read0187881/misc/27392008 西尾崇 新倉謙一 松尾保孝 秋田英万 小暮健太朗 原島秀吉 居城邦治 居城邦治 O‐GlcNAc提示量子ドット特異的細胞内挙動の観察 高分子学会北海道支部研究発表会講演要旨集 41st 31 null 20070206 read0187881/misc/24911404 Yamada Yuma Akita Hidetaka Kogure Kentaro Kamiya Hiroyuki Harashima Hideyoshi Mitochondrial drug delivery and mitochondrial disease therapy--an approach to liposome-based delivery targeted to mitochondria. Recent progress in genetics and molecular biology has provided useful information regarding the molecular mechanisms associated with the mitochondrial diseases. Genetic approaches were initiated in the late 1980s to clarify the gene responsible for various mitochondrial diseases, and information concerning genetic mutations is currently used in the diagnosis of mitochondrial diseases. Moreover, it was also revealed that mitochondria play a central role in apoptosis, or programmed cell death, which is closely related to the loss of physiological functions of tissues. Therefore, drug therapie... Elsevier Mitochondrion 1567-7249 7 1-2 63 71 20070200 10.1016/j.mito.2006.12.003 17296332 read0187881/misc/28810041 Kentaro Kogure Development of a novel artificial gene delivery system multifunctional envelope-type nano device for gene therapy YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 0031-6903 127 10 1685 1691 20070000 read0187881/misc/28809556 Ayman El-Sayed Ikramy A. Khalil Kentaro Kogure Hideyoshi Harashima Mechanism of membrane fusion between endosomal membrane and octaarginine or octalysine surface modified liposomes in live cells YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 0031-6903 127 87 88 20070000 read0187881/misc/28804603 Hidetaka Akita Asako Kudo Arisa Minoura Kentaro Kogure Hideyoshi Harashima Novel approach for the nuclear delivery of macromolecule via membrane fusion in non-dividing cell YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 0031-6903 127 85 86 20070000 read0187881/misc/28801470 Tomoko Higashi Yoshio Nakamura Hidetaka Akita Kentaro Kogure Hideyoshi Harashima Quantitative imaging of intracellular trafficking of siRNA vector YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 0031-6903 127 171 172 20070000 read0187881/misc/10972265 Hidetaka Akita 秋田 英万 Kentaro Kogure 小暮 健太朗 Hideyoshi Harashima 原島 秀吉 Development of multifunctional envelope-type nano-device (mend) based on the regulation of intracellular trafficking 【遺伝子・核酸医薬品のデリバリー】 多機能性エンベロープ型ナノ構造体(MEND) 日本DDS学会 Drug Delivery System 1881-2732 22 2 115 122 20070000 10.2745/dds.22.115 2-s2.0-85024454883 read0187881/misc/26295432 YAMADA Yuma AKITA Hidetaka KAMIYA Hiroyuki KOGURE Kentaro SHINOHARA Yasuo KOBAYASHI Hideo KIKUCHI Hiroshi HARASHIMA Hideyoshi Effective drug delivery toward mitochondria by combination of mitochondrial membrane fusion system and mitochondrial targeting signal peptide 日本ミトコンドリア学会年会要旨集 6th 31 null 20061213 read0187881/misc/26295404 山田勇磨 秋田英万 紙谷浩之 小暮健太朗 山本武範 篠原康雄 山下菊治 小林英夫 菊池寛 原島秀吉 膜融合を介してミトコンドリアへの高分子薬物送達を可能とする,``MITO‐Porter''の細胞内での膜融合能評価 Drug Deliv Syst 0913-5006 21 3 298 null 20060620 read0187881/misc/24911409 NAKAMURA Takashi MORIGUCHI Rumiko KOGURE Kentaro MINOURA Arisa MASUDA Tomoya AKITA Hidetaka KATO Kazunori HAMADA Hirofumi UENO Masaharu FUTAKI Shiroh HARASHIMA Hideyoshi Delivery of Condensed DNA by Liposomal Non-viral Gene Delivery System into Nucleus of Dendritic Cells(Biopharmacy) In this study, we developed novel double-membranous non-viral gene delivery system modified with SV-40 T antigen-derived nuclear localization signal (NLS-DMEND) for delivery of luciferase plasmid DNA to nucleus of non-dividing mouse bone marrow-derived dendritic cells (BMDC). Intracellular trafficking and gene expression of NLS-DMEND in the BMDC were evaluated. Condensed DNA was observed in the nucleus by confocal laser scanning microscopy, and the NLS-DMEND induced significant luciferase activity in the BMDC. It was suggested that the condensed DNA particle transferred into nucleus via ene... The Pharmaceutical Society of Japan 公益社団法人日本薬学会 Biological & pharmaceutical bulletin 0918-6158 29 6 1290 1293 20060601 10.1248/bpb.29.1290 read0187881/misc/39051662 山田 勇麿 秋田 英万 小暮 健太朗 紙谷 浩之 原島 秀吉 篠原 康雄 小林 英夫 菊池 寛 ミトコンドリアを標的としたDDSと疾患治療への応用 : ミトコンドリア融合性リポソーム，"MITO-Porter" を用いたアプローチ Drug delivery system 0913-5006 21 3 275 275 20060510 read0187881/misc/27392052 西尾崇 新倉謙一 松尾保孝 秋田英万 小暮健太朗 原島秀吉 居城邦治 居城邦治 糖鎖提示半導体ナノ微粒子の作製と細胞内ダイナミクス観察 高分子学会予稿集(CD-ROM) 55 1 Disk1 3PB192 null 20060510 read0187881/misc/24911464 原島 秀吉 秋田 英万 小暮 健太朗 多機能性エンベロープ型ナノ構造体による人工遺伝子デリバリーシステムの開発 Drug delivery system 0913-5006 21 3 null null 20060510 read0187881/misc/28907416 Tomoya Masuda Hidetaka Akita Takashi Nishio Kenichi Niikura Asako Kudo Kentaro Kogure Akitada Iwasa Kuniharu Ijiro Hideyoshi Harashima Development of Nuclear Targeting System of Plasmid DNA; Impact of Sugar Display on the Surface of Lipid Envelope MOLECULAR THERAPY 1525-0024 13 S388 S388 20060500 read0187881/misc/28901101 Hiroto Hatakeyama Hidetaka Akita Kentaro Kogure Motoi Oishi Yukio Nagasaki Hideo Kobayashi Hiroshi Kikuchi Hideyoshi Harashima Development of Novel Systemic Gene Delivery System for Cancer Therapy with Tumor-Specifically Cleavable PEG-Lipid MOLECULAR THERAPY 1525-0024 13 S203 S203 20060500 read0187881/misc/24911410 Nakamura Yoshio Kogure Kentaro Yamada Yuma Futaki Shiroh Harashima Hideyoshi Significant and prolonged antisense effect of a multifunctional envelope-type nano device encapsulating antisense oligodeoxynucleotide A multifunctional envelope-type nano device (MEND) was developed for use as an efficient non-viral system for the delivery of plasmid DNA (pDNA) using octaarginine (R8) as an internalizing ligand. Three types of R8-MENDs were prepared, co-encapsulating luciferase-encoding pDNA and antiluciferase oligodeoxynucleotide (ODN) condensed by three polycations, stearyl octaarginine (STR-R8), poly-L-lysine (PLL) and protamine, and the antisense effects of the ODN-encapsulated R8-MENDs (ODN-MEND) were analysed in-vitro. The ODN-MEND packaged using protamine as a condenser showed a 90% antisense effec... Royal Pharmaceutical Society of Great Britain Journal of Pharmacy and Pharmacology 0022-3573 58 4 431 437 20060400 10.1211/jpp.58.4.0002 read0187881/misc/10972266 小暮 健太朗 原島 秀吉 【先端生物医学研究・医療のための遺伝子導入テクノロジー ウイルスを用いない遺伝子導入法の材料,技術,方法論の新たな展開】 遺伝子導入のための材料 MEND (株)メディカルドゥ 遺伝子医学MOOK 1349-2527 5 90 94 20060400 read0187881/misc/26295408 小暮健太朗 小暮健太朗 中村宣央 中村宣央 山田勇磨 山田勇磨 原島秀吉 原島秀吉 オリゴデオキシヌクレオチドの機能向上を目指した多機能性エンベロープ型ナノ構造体への封入 日本薬学会年会要旨集 0918-9823 126th 2 138 null 20060306 read0187881/misc/26295416 工藤亜沙子 秋田英万 増田智也 山田勇磨 小暮健太朗 箕浦ありさ 原島秀吉 核膜融合に基づく新規核内送達デバイスの構築 薬剤学 0372-7629 66 Supplement 113 null 20060224 read0187881/misc/26295414 中村宜央 小暮健太朗 山田勇磨 原島秀吉 オリゴDNAの多機能性エンベロープ型ナノ構造体へのパッケージングの確立とその機能評価 薬剤学 0372-7629 66 Supplement 114 null 20060224 read0187881/misc/26295410 山田勇磨 秋田英万 紙谷浩之 小暮健太朗 篠原康雄 小林英夫 菊池寛 原島秀吉 ミトコンドリアを標的とする膜融合性リポソーム,MITO‐Porterを用いた生細胞ミトコンドリアへの高分子薬物送達―共焦点レーザースキャン顕微鏡を用いた細胞内動態観察― 薬剤学 0372-7629 66 Supplement 109 null 20060224 read0187881/misc/24911411 Moriguchi Rumiko Kogure Kentaro Iwasa Akitada Akita Hidetaka Hideyoshi Harashima Non-linear pharmacodynamics in a non-viral gene delivery system: Positive non-linear relationship between dose and transfection efficiency A remarkable non-linearity was found between dose and transfection activities of non-viral gene delivery systems, such as a Lipofectamine/DNA complex and an octaarginine-modified multifunctional envelope-type nano device (R8-MEND). We measured the nuclear delivery of pDNA to distinguish the non-linearity in intracellular pharmacokinetics or pharmacodynamics after transfection with R8-MEND at different doses. A remarkable positive non-linearity was found in the pharmacodynamics when the dose was increased. Even dummy pDNA enhanced the efficiency of transcription and/or translation per pDNA i... Elsevier B.V Journal of Controlled Release 0168-3659 110 3 605 609 20060200 10.1016/j.jconrel.2005.10.021 read0187881/misc/10972267 小暮 健太朗 秋田 英万 原島 秀吉 【ナノテクノロジーと医療 基礎・臨床研究と展望】 ナノテクノロジーによる遺伝子治療 エンベロープ型ナノ構造体による遺伝子デリバリー (株)日本臨床社 日本臨床 0047-1852 64 2 258 262 20060200 read0187881/misc/28906386 K. Fukuzawa K. Kogure S. Manabe A. Tokumura In vitro cytotoxicity of alpha-tocopheryl succinate, malonate and oxalate on cancer cells and their in vivo anti-cancer effects on mouse melanoma FREE RADICAL RESEARCH 1071-5762 40 S134 S134 20060000 read0187881/misc/27392070 NISHIO Takashi NIIKURA Kenichi MATSUO Yasutaka AKITA Hidetaka KOGURE Kentaro HARASHIMA Hideyoshi IJIRO Kuniharu IJIRO Kuniharu Syntheses of Sugar-displaying Quantum Dots as a Cellular Stress Sensing Probe Abstr RIES Hokudai Symp 8th 72-73 null 20060000 read0187881/misc/24911413 YAMADA Yuma KOGURE Kentaro NAKAMURA Yoshio INOUE Kaori AKITA Hidetaka NAGATSUGI Fumi SASAKI Shigeki SUHARA Tetsuya HARASHIMA Hideyoshi Development of Efficient Packaging Method of Oligodeoxynucleotides by a Condensed Nano Particle in Lipid Envelope Structure(Biopharmacy) An efficient delivery system is required if antisense oligodeoxynucleotides (ODN) are to be utilized for gene therapy. We report herein on the development of a novel ODN delivery system, ODN-encapsulated nano particles (ODN-ENP) using an efficient and simple packaging method. The ODN-ENP consists of a condensed ODN particle and a lipid envelope, which can be equipped with various functional devices for the efficient delivery of ODN with a small diameter (150nm). The encapsulation efficiency and ODN recovery of ODN-ENP were significantly higher than those of other packaging methods, such as ... The Pharmaceutical Society of Japan 公益社団法人日本薬学会 Biological & pharmaceutical bulletin 0918-6158 28 10 1939 1942 20051001 10.1248/bpb.28.1939 16204950 read0187881/misc/10972268 原島 秀吉 小暮 健太朗 秋田 英万 紙谷 浩之 【DDS研究の現状と将来展望】 遺伝子治療とDDS 多機能性リポソーム (株)じほう PHARM TECH JAPAN 0910-4739 21 12 2059 2063 20051000 read0187881/misc/27502110 後藤了 小暮健太朗 小松和志 宗像達夫 宇都義浩 堀均 赤須通範 寺田弘 Cepharanthine,tetrandrine,isotetrandrine分子の柔軟性とラジカル消去活性 分子構造総合討論会講演要旨集(CD-ROM) 2005 2P161 null 20050901 read0187881/misc/26295419 山田勇磨 秋田英万 小暮健太朗 紙谷浩之 篠原康雄 小林英夫 菊池寛 原島秀吉 膜融合を介してミトコンドリアへの送達を可能とするリポソーム,``Mitosome''の開発 Drug Deliv Syst 0913-5006 20 3 312 null 20050706 read0187881/misc/24911466 原島 秀吉 小暮 健太朗 秋田 英万 紙谷 浩之 多機能性エンベロープ型ナノ構造体の開発 : Programmed Packaging の提唱 Drug delivery system 0913-5006 20 3 null null 20050510 read0187881/misc/38804948 福澤 健治 真鍋 幸恵 小暮 健太朗 徳村 彰 2-A-7 マウスに移植した黒色腫瘍に対するα-トコフェロール誘導体の抗がん効果(第57回大会一般研究発表)(第57回大会研究発表要旨) 日本ビタミン学会 ビタミン 0006-386X 79 4 236 236 20050425 read0187881/misc/27538037 Nakamra T Kogure K Moriguchi R Futaki S Fujimuro M Yokosawa H Seya T Harashima H Development of a Novel Non-Viral DNA Vaccine-Delivering Gene into the Nucleus of Dendritic Cells. Mol.Therapy 11 S27-S28 null 20050000 read0187881/misc/26295437 YAMADA Yuma AKITA Hidetaka KAMIYA Hiroyuki KOGURE Kentaro SHINOHARA Yasuo KOBAYASHI Hideo KIKUCHI Hiroshi HARASHIMA Hideyoshi MITO-Porter, a new-type carrier for mitochondrial macromolecules delivery system via a membrane fusion mechanism 日本ミトコンドリア学会年会要旨集 5th 69 null 20050000 read0187881/misc/25953313 茶木伸治 小暮健太朗 二木史朗 丸山一雄 原島秀吉 PEG修飾リポソームの細胞内動態制御の検討 Drug Deliv Syst 0913-5006 19 3 270 null 20040510 read0187881/misc/28512138 K Kogure K Fukuzawa Tocopheryl succinate - Versatile functions due to its unique physicochemical properties JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION 1880-5086 35 1 29 34 20040000 read0187881/misc/10814285 Kentaro Kogure Kenji Fukuzawa Tocopheryl succinate-versatile function due to its unique physicochemical properties Journal of Clinical Biochemistry and Nutrition 1880-5086 35 1 29 34 20040000 10.3164/jcbn.35.29 read0187881/misc/10814284 K Fukuzawa Kenji Fukuzawa K Kogure Kentaro Kogure M Morita Motoki Morita S Hama Susumu Hama S Manabe Akira Tokumura A Tokumura Enhancement of nitric oxide and superoxide generations by alpha-tocopheryl succinate and its apoptotic and anticancer effects Enhancement of Nitric Oxide and Superoxide Generations by α-Tocopheryl Succinate and Its Apoptotic and Anticancer Effects BIOCHEMISTRY-MOSCOW Biochemistry (Moscow) 0006-2979 69 1 50 57 20040100 10.1023/B:BIRY.0000016351.77553.74 14972018 read0187881/misc/38664798 福澤 健治 中島 佐和 土江 明子 小暮 健太郎 徳村 彰 5.PAF様リン脂質酸化二次産物とビタミンE(第300回脂溶性ビタミン総合研究委員会研究発表要旨) 日本ビタミン学会 ビタミン 0006-386X 77 10 590 591 20031025 read0187881/misc/23015873 K Kogure S Manabe S Hama A Tokumura K Fukuzawa Potentiation of anti-cancer effect by intravenous administration of vesiculated alpha-tocopheryl hemisuccinate on mouse melanoma in vivo CANCER LETTERS 0304-3835 192 1 19 24 20030300 10.1016/S0304-3835(02)00683-3 read0187881/misc/10972269 小暮 健太朗 研究し尽くされたはずのビタミンE 次々と発見されるエステル体の生理作用とメカニズム Various effects of the most commonly used Vitamin E have been thought to be due to its antioxidative effect. Recently, α-tocopheryl succinate(Toco-S), an ester of Vitamin E, which lacks antioxidative ability, has been gaining considerable attention, because Toco-S has various biological activities such as induction of apoptosis and activation of protein kinase C. All of these effects of Toco-S are completely different from those of vitamin E. In this article, different effects of Vitamin E and Toco-S are described mainly based on their physical properties. (一社)日本生物物理学会 生物物理 0582-4052 43 2 70 74 20030300 10.2142/biophys.43.70 read0187881/misc/23015869 K Kogure S Hama S Manabe A Tokumura K Fukuzawa High cytotoxicity of alpha-tocopheryl hemisuccinate to cancer cells is due to failure of their antioxidative defense systems CANCER LETTERS 0304-3835 186 2 151 156 20021200 10.1016/S0304-3835(02)00344-0 read0187881/misc/23015870 T Nakamura T Ooi K Kogure M Nishimura H Terada T Kusumi Structures of the radical (DPPH) oxidation products of dihydrocapsaicin TETRAHEDRON LETTERS 0040-4039 43 45 8181 8183 20021100 10.1016/S0040-4039(02)01813-0 read0187881/misc/23015872 K Kogure S Goto M Nishimura M Yasumoto K Abe C Ohiwa H Sassa T Kusumi H Terada Mechanism of potent antiperoxidative effect of capsaicin BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 0304-4165 1573 1 84 92 20021000 10.1016/S0304-4165(02)00335-5 read0187881/misc/23015871 A Tokumura E Majima Y Kariya K Tominaga K Kogure K Yasuda K Fukuzawa Identification of human plasma lysophospholipase D, a lysophosphatidic acid-producing enzyme, as autotaxin, a multifunctional phosphodiesterase JOURNAL OF BIOLOGICAL CHEMISTRY 0021-9258 277 42 39436 39442 20021000 10.1074/jbc.M205623200 read0187881/misc/23015867 A Tokumura J Sinomiya S Kishimoto T Tanaka K Kogure T Sugiura K Satouchi K Waku K Fukuzawa Human platelets respond differentially to lysophosphatidic acids having a highly unsaturated fatty acyl group and alkyl ether-linked lysophosphatidic acids BIOCHEMICAL JOURNAL 0264-6021 365 617 628 20020800 10.1042/BJ20020348 read0187881/misc/23015868 K Kogure M Morita S Hama S Nakashima A Tokumura K Fukuzawa Enhancement by alpha-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-gamma through the upregulation of protein kinase C in rat vascular smooth muscle cells EUROPEAN JOURNAL OF BIOCHEMISTRY 0014-2956 269 9 2367 2372 20020500 10.1046/j.1432-1033.2002.02894.x read0187881/misc/38662045 小暮 健太朗 浜 進 真鍋 幸恵 鈴木 一郎 渋谷 雅之 徳村 彰 福澤 健治 トコフェロールコハク酸の生理作用に関与する構造的因子の検討 日本ビタミン学会 ビタミン 0006-386X 76 3 161 161 20020325 read0187881/misc/38661971 浜 進 小暮 健太朗 中島 佐和 真鍋 幸恵 徳村 彰 福澤 健治 トコフェロールコハク酸によるNO産生促進効果の機構解析 日本ビタミン学会 ビタミン 0006-386X 76 3 161 161 20020325 read0187881/misc/38661965 真鍋 幸恵 小暮 健太朗 浜 進 中島 佐和 徳村 彰 福澤 健治 黒色腫瘍に対するα-トコフェロールコハク酸の抗ガン効果 日本ビタミン学会 ビタミン 0006-386X 76 3 160 160 20020325 read0187881/misc/28605907 A Tokumura A Tsuchie K Kogure K Fukuzawa Cigarette smoking increases plasma level of phosphatidylcholine having an oxidatively shortened fatty acyl group with a carboxyl terminal FREE RADICAL BIOLOGY AND MEDICINE 0891-5849 33 S19 S19 20020000 read0187881/misc/28595410 K Kogure S Hama S Manabe M Kisaki A Tokumura K Fukuzawa High toxicity of alpha-tocopheryl succinate to cancer cells is due to their failed antioxidative defense system FREE RADICAL BIOLOGY AND MEDICINE 0891-5849 33 S173 S173 20020000 read0187881/misc/28581651 K Fukuzawa Y Saitoh K Akai K Kogure A Tokumura A Shibata Antioxidant effect of bovine serum albumin on iron-chelate/superoxide-dependent membrane lipid peroxidation FREE RADICAL BIOLOGY AND MEDICINE 0891-5849 33 S222 S222 20020000 read0187881/misc/25339497 濱 進 小暮健太朗 森田元喜 德村 彰 福澤健治 ラット血管平滑筋細胞におけるα-トコフェロールコハク酸の一酸化窒素 (NO) 産生促進作用にはプロテインキナーゼCが関与する ビタミンE研究の進歩X 85 89 20020000 read0187881/misc/23015866 A Tokumura K Tominaga K Yasuda Hideharu Kanzaki K Kogure K Fukuzawa Lack of significant differences in the corrected activity of lysophospholipase D, producer of phospholipid mediator lysophosphatidic acid, in incubated serum from women with and without ovarian tumors CANCER 0008-543X 94 1 141 151 20020100 10.1002/cncr.10146 read0187881/misc/10814287 土江 明子 Akira Tokumura ?村 彰 Kentaro Kogure 小暮 健太朗 Kenji Fukuzawa 福澤 健治 喫煙が血液中で悪玉リン脂質をつくるー動脈硬化との関連ー 日本薬学会 第122年会講演ハイライト 22 22 20020000 read0187881/misc/23015865 K Kogure M Morita S Nakashima S Hama A Tokumura K Fukuzawa Superoxide is responsible for apoptosis in rat vascular smooth muscle cells induced by alpha-tocopheryl hemisuccinate BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 0304-4165 1528 1 25 30 20010900 10.1016/S0304-4165(01)00168-4 read0187881/misc/29104768 K Fukuzawa A Tokumura K Kogure M Iemura N Gondoh M Fujii S Ueno A Shibata A comparative study of the ability of ferric nitrilotriacetate and other iron chelators to assist membrane lipid peroxidation by superoxide radicals (vol 110, pg 69, 2001) CHEMISTRY AND PHYSICS OF LIPIDS 0009-3084 111 2 195 195 20010600 read0187881/misc/38803571 小暮 健太朗 浜 進 中島 佐和 真鍋 幸恵 山内 泉 徳村 彰 福澤 健治 2-I-4 ビタミンEコハク酸によって誘導されるアポトーシスの機構解析 日本ビタミン学会 ビタミン 0006-386X 75 4 220 220 20010425 read0187881/misc/38803529 森田 元喜 小暮 健太朗 浜 進 中島 佐和 徳村 彰 福澤 健治 2-I-5 ビタミンEコハク酸によるNO産生促進機構の解析 日本ビタミン学会 ビタミン 0006-386X 75 4 221 221 20010425 read0187881/misc/23015863 K Fukuzawa A Tokumura K Kogure M Iemura N Gondoh M Fujii S Ueno A Shibata A comparative study of the ability of ferric nitriloacetate and other iron chelators to assist membrane lipid peroxidation by superoxide radicals CHEMISTRY AND PHYSICS OF LIPIDS 0009-3084 110 1 69 84 20010300 10.1016/S0009-3084(01)00127-X read0187881/misc/23015864 Efficient radical trapping at the surface and inside the phospholipid membrane is responsible for highly potent antiperoxidative actirity of the carotenoid astaxanthin. Biochimica et Biophysica Acta 0005-2728 1512 2 251 258 20010000 10.1016/S0005-2736(01)00326-1 read0187881/misc/23015862 T Chikawa T Ikata S Katoh Y Hamada K Kogure K Fukuzawa Preventive effects of lecithinized superoxide dismutase and methylprednisolone on spinal cord injury in rats: Transcriptional regulation of inflammatory and neurotrophic genes JOURNAL OF NEUROTRAUMA 0897-7151 18 1 93 103 20010100 read0187881/misc/10972270 上野 雅晴 小暮 健太朗 膜タンパク質の膜間移行現象とその応用 (一社)日本生物物理学会 生物物理 0582-4052 41 1 28 31 20010100 10.2142/biophys.41.28 read0187881/misc/23015861 K Kogure T Itoh O Okuda K Hayashi M Ueno The delivery of protein into living cells by use of membrane fusible erythrocyte ghosts INTERNATIONAL JOURNAL OF PHARMACEUTICS 0378-5173 210 1-2 117 120 20001200 10.1016/S0378-5173(00)00579-2 11163993 read0187881/misc/10972271 小暮 健太朗 福澤 健治 ビタミンEとアポトーシス (公社)日本ビタミン学会 ビタミン 0006-386X 74 9 481 483 20000900 10.20632/vso.74.9_481 read0187881/misc/23015860 A Tokumura T Sumida M Toujima K Kogure K Fukuzawa Y Takahashi S Yamamoto Structural identification of phosphatidylcholines having an oxidatively shortened linoleate residue generated through its oxygenation with soybean or rabbit reticulocyte lipoxygenase JOURNAL OF LIPID RESEARCH 0022-2275 41 6 953 962 20000600 read0187881/misc/38663421 森田 元喜 小暮 健太朗 真鍋 華子 徳村 彰 福澤 健治 2-I-29 ビタミンEコハク酸によるNO産生調節機構の解析 日本ビタミン学会 ビタミン 0006-386X 74 4 216 216 20000425 read0187881/misc/38663394 小暮 健太朗 森田 元喜 真鍋 華子 徳村 彰 福澤 健治 2-I-37 ビタミンEコハク酸エステル誘導アポトーシスへの活性酸素の関与 日本ビタミン学会 ビタミン 0006-386X 74 4 218 218 20000425 read0187881/misc/10814288 A Tokumura T Sumida M Toujima K Kogure K Fukuzawa Platelet-activating factor (PAF)-like oxidized phospholipids: Relevance to atherosclerosis BIOFACTORS 0951-6433 13 1-4 29 33 20000000 11237195 read0187881/misc/38665779 小暮 健太朗 福澤 健治 コハク酸α-トコフェロールに関する最近の話題 日本ビタミン学会 ビタミン 0006-386X 73 10 612 613 19991025 read0187881/misc/23015859 K Kogure O Okuda C Nakamura K Hayashi M Ueno Effects of incorporation of various amphiphiles into recipient liposome membranes on inter-membrane protein transfer CHEMICAL & PHARMACEUTICAL BULLETIN 0009-2363 47 8 1117 1120 19990800 10.1248/cpb.47.1117 10478466 read0187881/misc/38661801 福澤 健治 小暮 健太朗 徳村 彰 土屋 浩一郎 堤下 靖之 丹羽 峰雄 2-I-17 食餌性ビタミンEはLPSによるマウス肝NO合成酵素の誘導を増大する 日本ビタミン学会 ビタミン 0006-386X 73 4 273 273 19990425 read0187881/misc/38665813 小暮 健太朗 福澤 健治 動脈硬化症発症に対するビタミンE の防御効果 日本ビタミン学会 ビタミン 0006-386X 73 3 199 202 19990325 read0187881/misc/23015858 K Kogure S Goto K Abe C Ohiwa M Akasu H Terada Potent antiperoxidation activity of the bisbenzylisoquinoline alkaloid cepharanthine: the amine moiety is responsible for its pH-dependent radical scavenge activity BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 0304-4165 1426 1 133 142 19990100 10.1016/S0304-4165(98)00146-9 read0187881/misc/10972272 小暮 健太朗 福澤 健治 ビタミンEのbeyond antioxidant function:コハク酸α-トコフェロールに関する最近の話題 (公社)日本ビタミン学会 ビタミン 0006-386X 72 10 581 583 19981000 10.20632/vso.72.10_581 read0187881/misc/23015857 K Kogure H Sassa K Abe K Kitahara Y Sano H Kawano Y Nakagawa H Terada Inhibitory effects of pentamethine trinuclear cyanine dyes on ADP/Fe2+-induced lipid peroxidation in rat liver mitochondria: Changes in the mode of action with the hydrophobic nature of the dyes BIOLOGICAL & PHARMACEUTICAL BULLETIN 0918-6158 21 2 180 183 19980200 10.1248/bpb.21.180 9514617 read0187881/misc/23015856 K Kogure C Nakamura O Okuda K Hayashi M Ueno Effect of dicetylphosphate or stearic acid on spontaneous transfer of protein from influenza virus-infected cells to dimyristoylphosphatidylcholine liposomes BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 0005-2736 1329 1 174 182 19971000 read0187881/misc/23015855 K Kogure O Okuda T Itoh K Hayashi M Ueno Development of a membrane fusible drug carrier from erythrocytes by the spontaneous transfer of viral fusion protein from influenza virus-infected cells BIOLOGICAL & PHARMACEUTICAL BULLETIN 0918-6158 20 5 581 583 19970500 10.1248/bpb.20.581 9178945 read0187881/misc/23015854 K Kogure K Yamamoto E Majima Y Shinohara K Yamashita H Terada Alteration of enzyme function of the type II hexokinase C-terminal half on replacements of restricted regions by corresponding regions of glucokinase JOURNAL OF BIOLOGICAL CHEMISTRY 0021-9258 271 25 15230 15236 19960600 10.1074/jbc.271.25.15230 8662949 read0187881/misc/23015853 Advanced research on liposome for drug delivery system. Fragrance Journal 24 4 66 null 19960000 read0187881/misc/23015852 K KOGURE M ITOH K HAYASHI M UENO SPONTANEOUS TRANSFER OF VIRAL PROTEIN FROM MEMBRANE OF INFLUENZA VIRUS-INFECTED CELLS TO LIPOSOMES IS DEPENDENT ON THE DIAMETER OF RECEIVER BIOLOGICAL & PHARMACEUTICAL BULLETIN 0918-6158 18 8 1168 1170 19950800 10.1248/bpb.18.1168 8535420 read0187881/misc/23015844 J ICHIHARA Y SHINOHARA K KOGURE H TERADA NUCLEOTIDE-SEQUENCE OF THE 5'-FLANKING REGION OF THE RAT TYPE-II HEXOKINASE GENE BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 0167-4781 1260 3 365 368 19950200 read0187881/misc/23015846 H SASSA K KOGURE Y TAKAISHI H TERADA STRUCTURAL BASIS OF POTENT ANTIPEROXIDATION ACTIVITY OF THE TRITERPENE CELASTROL IN MITOCHONDRIA - EFFECT OF NEGATIVE MEMBRANE-SURFACE CHARGE ON LIPID-PEROXIDATION FREE RADICAL BIOLOGY AND MEDICINE 0891-5849 17 3 201 207 19940900 read0187881/misc/23015845 Y SHINOHARA K YAMAMOTO K KOGURE J ICHIHARA H TERADA STEADY-STATE TRANSCRIPT LEVELS OF THE TYPE-II HEXOKINASE AND TYPE-1 GLUCOSE-TRANSPORTER IN HUMAN TUMOR-CELL LINES CANCER LETTERS 0304-3835 82 1 27 32 19940700 read0187881/misc/23015847 H KAWANO K KOGURE K FUKUZAWA H TERADA EFFECT OF INORGANIC-PHOSPHATE ON IRON-INDUCED LIPID-PEROXIDATION IN RAT-LIVER MITOCHONDRIA BIOLOGICAL & PHARMACEUTICAL BULLETIN 0918-6158 16 11 1094 1098 19931100 10.1248/bpb.16.1094 8312862 read0187881/misc/23015843 K KOGURE K FUKUZAWA H KAWANO H TERADA SPERMINE ACCELERATES IRON-INDUCED LIPID-PEROXIDATION IN MITOCHONDRIA BY MODIFICATION OF MEMBRANE-SURFACE CHARGE FREE RADICAL BIOLOGY AND MEDICINE 0891-5849 14 5 501 507 19930500 read0187881/misc/23015842 K KOGURE Y SHINOHARA H TERADA EVOLUTION OF THE TYPE-II HEXOKINASE GENE BY DUPLICATION AND FUSION OF THE GLUCOKINASE GENE WITH CONSERVATION OF ITS ORGANIZATION JOURNAL OF BIOLOGICAL CHEMISTRY 0021-9258 268 12 8422 8424 19930400 read0187881/misc/23015851 The photosensitizing dyes as antioxidants Fragrance Journal 21 100 null 19930000 read0187881/misc/23015850 Accelerating effect on lipid peroxidation of rat liver mitochondria by the addition of the polyamine(共著) Lipid peroxide research 16 39 null 19920000 read0187881/misc/23015848 H KAWANO K FUKUZAWA K KOGURE H TERADA INITIATION OF LIPID-PEROXIDATION IN RAT-LIVER MITOCHONDRIA BY CHELATED IRON-OXYGEN COMPLEXES JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION 1880-5086 11 1 21 30 19910700 10.3164/jcbn.11.21 read0187881/misc/10972273 小暮 健太朗 寺田 弘 微小差スペクトルによる生物試料の分析 (株)永井書店 病態生理 0286-2190 9 11 858 863 19901100 read0187881/misc/23015849 Detection of biological samples having Spectrophoto-metrically similar natures(共著) 9 11 858 null 19900000 read0187881/presentations/22486255 Kohki Tachibana 立花 洸季 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 Tatsuhiro Ishida 石田 竜弘 Keiichiro Okuhira 奥平 桂一郎 HDL構成タンパク質分泌に対するスフィンゴシン-1-リン酸及びフィンゴリモドの影響 第57回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会 20181110 read0187881/presentations/22486256 Kohki Tachibana 立花 洸季 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 Tatsuhiro Ishida 石田 竜弘 Keiichiro Okuhira 奥平 桂一郎 HDL構成タンパク質分泌に対するスフィンゴシン-1-リン酸及びフィンゴリモドの影響 第91回日本生化学会大会 20180924 read0187881/presentations/22486266 Tasuku Torao 虎尾 祐 大島 康史 三村 美夕紀 藤川 昂樹 Tatsuya Fukuta 福田 達也 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 微弱電流処理によるユニークなエンドサイトーシス誘導に関連する因子の検討 日本薬学会138年会 20180326 read0187881/presentations/22486265 三橋 亮介 梶本 和昭 Tatsuya Fukuta 福田 達也 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 トコフェロールコハク酸含有リポソームによる脂肪蓄積の抑制作用 日本薬学会138年会 20180326 read0187881/presentations/22486263 大島 康史 Hasan Mahadi Noriko Tarashima 田良島 典子 濱 進 Tatsuya Fukuta 福田 達也 Tamotsu Tanaka 田中 保 Noriaki Minakawa 南川 典昭 Kentaro Kogure 小暮 健太朗 微弱電流処理を利用した機能性核酸の細胞内取り込みの検討 日本薬学会138年会 20180326 read0187881/presentations/22486262 三村 美夕紀 大島 康史 Tasuku Torao 虎尾 祐 藤川 昂樹 Tatsuya Fukuta 福田 達也 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 微弱電流処理により誘導される細胞取り込み過程の定量的評価 日本薬学会138年会 20180326 read0187881/presentations/22486261 石川 みすず 平井 将太 ? 進 細井 信造 Tatsusada Yoshida 吉田 達貞 ? 橋 侑 Kentaro Kogure 小暮 健太朗 アスタキサンチンとα-トコトリエノールの相乗的抗酸化効果における立体構造の影響 日本薬学会138年会 20180326 read0187881/presentations/22486260 平井 将太 ?橋 侑 Tamotsu Tanaka 田中 保 Tatsuya Fukuta 福田 達也 Tatsusada Yoshida 吉田 達貞 Kentaro Kogure 小暮 健太朗 アスタキサンチンと抗酸化物質の組合せによる相乗的な活性酸素消去活性の向上. 日本薬学会138年会 20180326 read0187881/presentations/22486259 賀川 真夕子 Tatsuya Fukuta 福田 達也 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 イオントフォレシスによる肝臓への核酸医薬送達 日本薬学会138年会 20180326 read0187881/presentations/22486258 M Hasan S Hama Hiroyuki Saito Kentaro Kogure Mechanistic analysis of the intracellular delivery of siRNA mediated by faint electric treatment 日本薬学会138年会 20180326 read0187881/presentations/22486257 Tatsuya Fukuta T Asai Kentaro Kogure N Oku Treatment of ischemic stroke by combination therapy with liposomal neuroprotectants and tissue plasminogen activator 日本薬学会138年会 20180326 read0187881/presentations/22486267 森戸 克弥 清水 良多 北村 苗穂子 朴 時範 岸野 重信 小川 順 Tatsuya Fukuta 福田 達也 Kentaro Kogure 小暮 健太朗 Tamotsu Tanaka 田中 保 乳酸菌が産生する希少脂肪酸のペルオキシソームにおける代謝 日本農芸化学会 2018年度大会 20180315 read0187881/presentations/22486269 三橋 亮介 Tatsuya Fukuta 福田 達也 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 トコフェロールコハク酸含有リポソームによる脂肪細胞の脂肪蓄積抑制効果 第29回ビタミンE研究会 20180127 read0187881/presentations/22486268 石川 みすず 平井 将太 ? 進 細井 信造 Tatsusada Yoshida 吉田 達貞 ? 橋 侑 Kentaro Kogure 小暮 健太朗 トコトリエノールとアスタキサンチンの相乗的抗酸化効果への立体構造の影響 第29回ビタミンE研究会 20180127 read0187881/presentations/22486270 Kentaro Kogure 小暮 健太朗 石川 みすず 平井 将太 ? 進 Tatsusada Yoshida 吉田 達貞 ?橋 侑 細井 信造 Tatsuya Fukuta 福田 達也 Tamotsu Tanaka 田中 保 α-トコトリエノールとアスタキサンチンの相乗的抗酸化効果 第357回脂溶性ビタミン総合研究委員会 20171215 read0187881/presentations/22486274 辻 和樹 島田 明奈 宮崎 徹 高橋 尚子 伊賀 永里奈 Rahman Md Motiur Michiyasu Nakao 中尾 允泰 Shigeki Sano 佐野 茂樹 Kentaro Kogure 小暮 健太朗 Tamotsu Tanaka 田中 保 種々の脂肪酸残基を有するセラミドの化学合成とその生理活性 第40回日本分子生物学会年会，第90回日本生化学会大会 プログラム 20171206 read0187881/presentations/22486273 Afroz Sheuli Katsuya Morito Kouki Fujikawa Ayano Yagi Kazunori Toida Emi Kiyokage Kentaro Kogure Shiro Watanabe Akira Tokumura Tamotsu Tanaka Antiulcer effect of lysophosphatidic acid-rich medicinal herbs and its mechanism 第40回日本分子生物学会年会，第90回日本生化学会大会 プログラム 20171206 read0187881/presentations/22486272 Md Motiur Rahman Erina Iga Akina Shimada Tohru Miyazaki Naoko Takahashi Mina Fujiwara Kazuki Tsuji Kentaro Kogure Tamotsu Tanaka Neuroprotective activity of phytoceramide 1-phosphate on serum deprivation-induced apoptosis of Neuro2a cells 第40回日本分子生物学会年会，第90回日本生化学会大会 プログラム 20171206 read0187881/presentations/22486271 清水 良多 山下 量平 伊賀 永里奈 Md. Motiur Rahman 東 桃代 Kentaro Kogure 小暮 健太朗 Tamotsu Tanaka 田中 保 液体クロマトグラフィー/タンデム質量分析によるヒト血漿中のセラミド及びセラミド 1-リン酸の解析 第40回日本分子生物学会年会，第90回日本生化学会大会 プログラム 20171206 read0187881/presentations/22486275 Kentaro Kogure 小暮 健太朗 大島 康史 Tasuku Torao 虎尾 祐 三村 美夕紀 藤川 昂樹 Hasan Mahadi 濱 進 Tatsuya Fukuta 福田 達也 Noriko Tarashima 田良島 典子 Tamotsu Tanaka 田中 保 Noriaki Minakawa 南川 典昭 微弱電流処理による高分子医薬の細胞質送達と機能発現 第39回生体膜と薬物の相互作用シンポジウム 20171026 read0187881/presentations/22486282 大島 康史 虎尾 祐 三村 美夕紀 Hasan Mahadi Noriko Tarashima 田良島 典子 濱 進 Tatsuya Fukuta 福田 達也 Tamotsu Tanaka 田中 保 Noriaki Minakawa 南川 典昭 Kentaro Kogure 小暮 健太朗 ユニークなエンドサイトーシスを誘起する微弱電流を利用した機能性核酸の細胞質送達 第56回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会 20171021 read0187881/presentations/22486281 賀川 真夕子 Tatsuya Fukuta 福田 達也 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 微弱電流処理によるsiRNAの細胞内送達と肝細胞遺伝子発現制御 第56回日本薬学会・日本薬剤師会・日本病院薬剤師会 20171021 read0187881/presentations/22486280 三村 美夕紀 大島 康史 虎尾 祐 藤川 昂樹 Hasan Mahadi 濱 進 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 微弱電流処理により誘起される細胞内取り込みの評価 第56回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会 20171021 read0187881/presentations/22486279 Tasuku Torao 虎尾 祐 大島 康史 三村 美夕紀 藤川 昂樹 Tatsuya Fukuta 福田 達也 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 微弱電流処理によるユニークなエンドサイトーシス誘導機構の検討 第56回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会 20171021 read0187881/presentations/22486278 三橋 亮介 Tatsuya Fukuta 福田 達也 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 トコフェロールコハク酸含有リポソームによる脂肪蓄積減少効果の検討 第56回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会 20171021 read0187881/presentations/22486277 石川 みすず 平井 将太 濱 進 細井 信造 Tatsusada Yoshida 吉田 達貞 高橋 侑 Kentaro Kogure 小暮 健太朗 相乗的抗酸化効果を示すアスタキサンチンとα-トコトリエノールのリポソーム膜における至適比率の検討 第56回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会 20171021 read0187881/presentations/22486276 平井 将太 石川 みすず 高橋 侑 Kentaro Kogure 小暮 健太朗 アスタキサンチンとカプサイシンの組合せによる相乗的な抗酸化活性の向上 第56回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会 20171021 read0187881/presentations/22486284 島田 明奈 Tohru Miyazaki 宮? 徹 高橋 尚子 Rahman Motiur Md 清水 良多 辻 和樹 山下 量平 Shigeki Sano 佐野 茂樹 Michiyasu Nakao 中尾 允泰 Tatsuya Fukuta 福田 達也 Kentaro Kogure 小暮 健太朗 Tamotsu Tanaka 田中 保 極長鎖脂肪酸およびこれを含有するセラミドのアポトーシス抑制活性 セラミド研究会 20171019 read0187881/presentations/22486283 Tamotsu Tanaka 田中 保 山下 量平 清水 良多 森戸 克弥 Md. Motiur Rahman 伊賀 永里奈 島田 明奈 福田 達也 Kentaro Kogure 小暮 健太朗 血液中のセラミド 1-リン酸の分子種組成と生物活性 セラミド研究会 予稿集 20171019 read0187881/presentations/22486233 Kentaro Kogure Noriko Tarashima K Fujikawa Y Oshima Tasuku Torao M Mimura M Hasan S Hama Tamotsu Tanaka Hiroyuki Saito Noriaki Minakawa Effective cellular delivery of intelligent shRNA expression device by faint electricity. The 5th Seminar of pharmaceutial sciences and technology. 20170923 read0187881/presentations/22486286 S Afroz Katsuya Morito K Fujikawa A Yagi Teru Ikoma E Kiyokage K Toida Kentaro Kogure Tamotsu Tanaka Phosphatidic acid-rich cereals as anti-ulcer foods and their mechanisms of action 日本脂質栄養学会第26回大会 20170922 read0187881/presentations/22486285 Md Motiur Rahman Erina Iga Tohru Miyazaki Naoko Takahashi MIna Fujiwara Kazuki Tsuji Kentaro Kogure Tamotsu Tanaka Phytoceramide 1-phosphate in vegetables and its anti-apoptotic effect in animal cells 日本脂質栄養学会第26回大会 予稿集 20170922 read0187881/presentations/22486234 Kentaro Kogure K Kigasawa S Hama K Kajimoto Transdermal delivery of liposomes encapsulating functional proteins by iontophoresis ILS/LRD Liposome Advances Combined Conference 20170916 read0187881/presentations/22486235 Tamotsu Tanaka M Md Rahman E Iga R Yamashita R Shimizu K Tsuji A Shimada Michiyasu Nakao Shigeki Sano Kentaro Kogure Plasma level of ceramide 1-phosphate and its anti-apoptotic activity. 58th International conference on the bioscience of lipids (ICBL) 20170911 read0187881/presentations/22486287 大島 康史 虎尾 祐 三村 美夕紀 藤川 昂樹 Hasan Mahadi 濱 進 Tatsuya Fukuta 福田 達也 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 微弱電流が誘起するユニークなエンドサイトーシスによる核酸の細胞質送達 遺伝子・デリバリー研究会第17回夏期セミナー 20170903 read0187881/presentations/22486288 Kentaro Kogure 小暮 健太朗 賀川 真夕子 大島 康史 Tasuku Torao 虎尾 祐 三村 美夕紀 Tatsuya Fukuta 福田 達也 Mahadi Hasan 濱 進 Tamotsu Tanaka 田中 保 微弱電流による核酸医薬の細胞内送達 第26回DDSカンファランス 20170900 read0187881/presentations/22486289 Kentaro Kogure 小暮 健太朗 賀川 真夕子 大島 康史 Tasuku Torao 虎尾 祐 三村 美夕紀 藤川 昂樹 Tatsuya Fukuta 福田 達也 微弱電流誘導性エンドサイトーシスによるsiRNAの細胞内送達と肝細胞遺伝子発現制御 日本核酸医薬学会第3回年会 20170712 read0187881/presentations/22486291 Noriaki Minakawa 南川 典昭 Noriko Tarashima 田良島 典子 Tomoki Takahashi 高橋 知樹 Seigi Yamamoto 山本 清義 Nozomi Kinjoh 金城 望 Hidenori Andou 安藤 英紀 Tatsuhiro Ishida 石田 竜弘 Kentaro Kogure 小暮 健太朗 化学修飾DNAを利用したRNA創薬 第33回日本DDS学会学術集会 20170706 read0187881/presentations/22486290 虎尾 祐 三村 美夕紀 大島 康史 藤川 昂樹 Hasan Mahadi 濱 進 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 微弱電流処理によるユニークなエンドサイトーシスの解析 第33回日本DDS学会学術集会 20170706 read0187881/presentations/22486292 Kentaro Kogure 小暮 健太朗 微弱電流によるナノ粒子の皮内デリバリー 第35回物性物理化学研究会 20170705 read0187881/presentations/22486293 Kentaro Kogure 小暮 健太朗 石川 みすず 平井 将太 ? 進 細井 信造 Tatsusada Yoshida 吉田 達貞 山下 栄次 橋 侑 ビタミンEとの相乗的抗酸化効果におけるアスタキサンチン立体構造の影響 第70回日本酸化ストレス学会学術集会 20170627 read0187881/presentations/22486294 宮城 諒 喜田 孝史 辻 和樹 Kentaro Kogure 小暮 健太朗 Tamotsu Tanaka 田中 保 グリコシルイノシトールホスホセラミドの抽出と精製 日本農芸化学会中四国支部第48回公演会 要旨集 20170617 read0187881/presentations/22486296 喜田 孝史 Aoi Itoh 伊藤 葵 木村 朱里 松岡 久嗣 藤原 美奈 辻 和樹 Kentaro Kogure 小暮 健太朗 Tamotsu Tanaka 田中 保 植物におけるグリコシルイノシトールホスホセラミド特異的ホスホリパーゼD活性の分布と性質 第59回日本脂質生化学会 20170615 read0187881/presentations/22486295 Tamotsu Tanaka 田中 保 Md Md Motiur Rahaman Motiur Rahaman 伊賀 永理奈 山下 量平 清水 良多 辻 和樹 島田 明奈 中尾 允泰 Michiyasu Nakao 佐野 茂樹 Shigeki Sano 小暮 健太朗 Kentaro Kogure 血液中に存在する極長鎖セラミド-1-リン酸のアポトーシス抑制作用 第59回日本脂質生化学会, 講演要旨集 20170615 read0187881/presentations/22486297 平井 将太 石川 みすず 渋谷 菜摘 ? 進 細井 信造 ? 橋 侑 山下 栄次 Kentaro Kogure 小暮 健太朗 トコトリエノールとアスタキサンチンの相乗的な抗酸化活性向上における至適比率の検討 日本ビタミン学会第69回大会 20170609 read0187881/presentations/22486299 大島 康史 Tasuku Torao 虎尾 祐 三村 美夕紀 藤川 昂樹 Hasan Mahadi 濱 進 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 微弱電流により誘起されるユニークなエンドサイトーシスの解析 遺伝子・デリバリー研究会第17回シンポジウム 20170527 read0187881/presentations/22486298 Kentaro Kogure 小暮 健太朗 藤川 昂樹 Hasan Mahadi 濱 進 Tamotsu Tanaka 田中 保 Noriko Tarashima 田良島 典子 Noriaki Minakawa 南川 典昭 微弱電流による新規核酸iRedの細胞内送達と遺伝子発現制御 遺伝子・デリバリー研究会第17回シンポジウム 20170527 read0187881/presentations/22486236 Kentaro Kogure Noriko Tarashima K Fujikawa Y Oshima Tasuku Torao M Mimura M Hasan S Hama Tamotsu Tanaka Hiroyuki Saito Noriaki Minakawa Effective cellular delivery of intelligent shRNA expression device by faint electricity. 6th FIP Pharmaceutical Sciences World Congress (PSWC) 20170521 read0187881/presentations/22486301 島田 明奈 伊賀 永里奈 Rahman Motiur Md 山下 量平 清水 良多 Kentaro Kogure 小暮 健太朗 Tamotsu Tanaka 田中 保 セラミド-1-リン酸のアポトーシス抑制活性 第58回日本生化学会中国・四国支部例会 20170520 read0187881/presentations/22486300 清水 良多 山下 量平 伊賀 永里奈 Rahman Motiur Md Momoyo Azuma 東 桃代 Kentaro Kogure 小暮 健太朗 Tamotsu Tanaka 田中 保 液体クロマトグラフィー/タンデム質量分析による血漿中のセラミド及びセラミド-1-リン酸の解析 第58回日本生化学会中国・四国支部例会 20170520 read0187881/presentations/22486237 M Md Rahman A Shimada Tohru Miyazaki K Tsuji Michiyasu Nakao Shigeki Sano Kentaro Kogure Tamotsu Tanaka Characterization of the Biological Effects of Ceramide-1-Phosphate. 第58回日本生化学会中国・四国支部例会 20170520 read0187881/presentations/22486302 三村 美夕紀 大島 康史 虎尾 祐 藤川 昂樹 Hasan Mahadi 濱 進 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 微弱電流処理によって誘起されるユニークなエンドサイトーシス 日本薬剤学会第32年会 20170511 read0187881/presentations/22486303 Taiki Kohiki 古曳 泰規 Masaya Denda 傳田 将也 藤川 昴樹 Tsubasa Inokuma 猪熊 翼 Akira Shigenaga 重永 章 Kentaro Kogure 小暮 健太朗 Akira Otaka 大? 章 N-Sulfanylethylanilideを用いた細胞内標的タンパク質ラベル化法の開発 日本薬学会第137年会 20170326 read0187881/presentations/22486304 Kentaro Kogure 小暮 健太朗 微弱電流による高分子物質皮膚透過促進と組織・細胞生理の変化 日本薬学会第137年会 20170325 read0187881/presentations/22486305 Ryo Funashiro 船城 凌 Natsumi Shibuya 渋谷 菜摘 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 Keiichiro Okuhira 奥平 桂一郎 HepG2細胞でのスフィンゴシン1リン酸(S1P)によるアポリポタンパク質A-I(apoA-I)発現の抑制 日本薬学会第137年会 20170324 read0187881/presentations/22486306 Kentaro Kogure 小暮 健太朗 Mahadi Hasan Noriko Tarashima 田良島 典子 Kohki Fujikawa 藤川 昂樹 濱 進 Tamotsu Tanaka 田中 保 樫田 啓 浅沼 浩之 Hiroyuki Saito 斎藤 博幸 Noriaki Minakawa 南川 典昭 微弱電流による機能性核酸の効率的な細胞質送達 日本核酸医薬学会第2回年会(東京) 20161200 read0187881/presentations/22486362 Kentaro Kogure 小暮 健太朗 抗酸化粒子による活性酸素消去と組織・細胞生理制御による生体内送達. 第619回 新潟薬科大学 薬学総合セミナー(新潟) 20161100 read0187881/presentations/22486308 Kohki Fujikawa 藤川 昂樹 Hasan Mahadi 濱 進 Tamotsu Tanaka 田中 保 Hiroyuki Saito 斎藤 博幸 Kentaro Kogure 小暮 健太朗 微弱電流処理による高分子物質の細胞質送達. 第38回生体膜と薬物の相互作用シンポジウム(名古屋) 20161100 read0187881/presentations/22486307 Kentaro Kogure 小暮 健太朗 亀崎 ちひろ 渋谷 菜摘 石川 みすず 中島 愛美 石橋 博 ? 進 細井 信造 山下 栄次 トコトリエノールとの抗酸化併用効果. 第12回アスタキサンチン研究会(富山) 20161100 read0187881/presentations/22486240 S Hama S Itakura R Matsui Kentaro Kogure Development of liposomal siRNA carriers using slightly acidic pH-sensitive peptide SAPSP for cancer therapy. 3rd International Conference on Biomaterials Science in Tokyo (ICBS2016) (Tokyo, Japan) 20161100 read0187881/presentations/22486239 Kohki Fujikawa M Hasan S Hama Tamotsu Tanaka Kentaro Kogure Faint electric treatment induces cytoplasmic delivery of functional macromolecules via changing endosome property. 3rd International Conference on Biomaterials Science in Tokyo (ICBS2016) (Tokyo, Japan) 20161100 read0187881/presentations/22486238 Kentaro Kogure Kohki Fujikawa M Hasan S Hama H Kashida H Asanuma Effective cytoplasmic delivery of functional macromolecules by faint electricity. 3rd International Conference on Biomaterials Science in Tokyo (ICBS2016) (Tokyo, Japan) 20161100 read0187881/presentations/22486310 Kohki Fujikawa 藤川 昂樹 Hasan Mahadi 濱 進 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 細胞のエンドソーム物性変化を誘導する微弱電流処理. 第55回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会(岡山) 20161000 read0187881/presentations/22486309 Ayano Yagi 屋宜 亜耶乃 Afroz Sheuli 生駒 照 Akira Tokumura ?村 彰 Kentaro Kogure 小暮 健太朗 Tamotsu Tanaka 田中 保 食物中のホスファチジン酸の抗胃潰瘍効果とホスホリパーゼA2活性化作用. 第55回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会(岡山) 20161000 read0187881/presentations/22486242 Kentaro Kogure Kohki Fujikawa M Hasan S Hama Effective Cytoplasmic Delivery of Macromolecules by Faint Electric Treatment 12th France-Japan Drug Delivery Systems Symposium (Cernay-la-Ville, France) 20161000 read0187881/presentations/22486241 S Hama S Itakura Kentaro Kogure Slightly Acidic pH Sensitive Peptide-Modified Nanoparticles for Nucleic Acid Delivery to Cancer Cells. BITs 6th Annual World Congress of Nano Science & Technology (Singapore) 20161000 read0187881/presentations/22486311 Kentaro Kogure 小暮 健太朗 亀崎 ちひろ 中島 愛美 石橋 博 ? 進 細井 信造 山下 栄次 アスタキサンチンとビタミンE共存による抗酸化活性の向上. 第69回日本酸化ストレス学会学術年会(仙台) 20160900 read0187881/presentations/22486313 松井 諒 鈴木 智子 板倉 祥子 Kentaro Kogure 小暮 健太朗 Hiroyuki Saito 斎藤 博幸 濱 進 微小環境応答性ドラッグデリバリーシステムの腫瘍内透過性の改善. 第14回 がんとハイポキシア研究会(岐阜) 20160800 read0187881/presentations/22486312 中島 采香 西 貴之 Kenji Fukuzawa 福澤 健治 Kentaro Kogure 小暮 健太朗 Hiroyuki Saito 斎藤 博幸 濱 進 多面的な抗癌作用を有するビタミンE誘導体から構成されるナノ粒子による薬剤耐性の克服. 第14回 がんとハイポキシア研究会(岐阜) 20160800 read0187881/presentations/22486317 松井 諒 濱 進 鈴木 智子 板倉 祥子 Kentaro Kogure 小暮 健太朗 Hiroyuki Saito 斎藤 博幸 腫瘍内透過性と微弱低pH応答性を併せ持つ薬物キャリアーの開発. 第32回日本DDS学会学術集会(静岡) 20160700 read0187881/presentations/22486316 扇田 隆司 林 直樹 福田 昂平 籾山 京子 Kentaro Kogure 小暮 健太朗 後藤 直正 Hiroyuki Saito 斎藤 博幸 細菌?型分泌装置のエフェクター輸送機構解明のための回転-分泌相関の検討. 第14回次世代を担う若手のためのフィジカル・ファーマフォーラム (大阪) 20160700 read0187881/presentations/22486315 山下 栄次 Kentaro Kogure 小暮 健太朗 ビタミンE とアスタキサンチンの相乗的抗酸化作用. 第30回カロテノイド研究談話会(沖縄) 20160700 read0187881/presentations/22486314 扇田 隆司 林 直樹 上川 翼 籾山 京子 福田 昂平 Kentaro Kogure 小暮 健太朗 後藤 直正 Hiroyuki Saito 斎藤 博幸 細菌?型分泌機構の解明を目指した分泌装置の回転―分泌相関の検討. 第11回トランスポーター研究会年会(JTRA2016)(京都) 20160700 read0187881/presentations/22486363 Kentaro Kogure 小暮 健太朗 ナノ粒子製剤の構築と微弱電流による非侵襲的投与法の開発 第53回薬剤学懇談会研究討論会 20160609 read0187881/presentations/22486320 西 貴之 濱 進 西本 明功 鈴木 智子 Hiroyuki Saito 斎藤 博幸 Kenji Fukuzawa 福澤 健治 Kentaro Kogure 小暮 健太朗 腹腔内貯留型トコフェロールコハク酸含有ナノ粒子の腹膜播種治療への応用. 日本ビタミン学会第68回大会(富山) 20160600 read0187881/presentations/22486319 Kentaro Kogure 小暮 健太朗 亀崎 ちひろ 中島 愛美 石橋 博 ? 進 細井 信造 山下 栄次 ビタミンEとアスタキサンチン共存による抗酸化作用の向上効果. 日本ビタミン学会第68回大会(富山) 20160600 read0187881/presentations/22486318 Hasan Mahadi Noriko Tarashima Kohki Fujikawa Takashi Ohgita Susumu Hama Tamotsu Tanaka Hiroyuki Saito Noriaki Minakawa Kentaro Kogure Intracellular delivery of a novel functional nucleic acid iRed by faint electric treatment for effective regulation of target genes 第32回DDS学術集会 20160600 read0187881/presentations/22486322 T Ohgita K Fukuda K Momiyama N Hayashi Kentaro Kogure 小暮 健太朗 N Gotoh Hiroyuki Saito 斎藤 博幸 Needle-like type III secretion apparatus regulates effector transport by rotational motion. 第55回日本生物物理学会年会(つくば) 20160500 read0187881/presentations/22486321 Kohki Fujikawa 藤川 昂樹 Hasan Mahadi 濱 進 Tamotsu Tanaka 田中 保 Kentaro Kogure 小暮 健太朗 微弱電流処理によって誘起されるエンドサイトーシスの解析. 日本薬剤学会第31年会(岐阜) 20160500 read0187881/presentations/22486323 Kentaro Kogure 小暮 健太朗 M ?i 進 ビタミンEコハク酸の多彩な生理作用と薬学への展開 日本薬学会136年会シンポジウム「ビタミンのケミカルバイオロジー研究」 20160327 read0187881/presentations/22486243 Kentaro Kogure Noninvasive and effective transdermal delivery of macromolecular medicines by faint electric treatment. 32nd International Annual Meeting in Pharmaceutical Sciences held by the Chulalongkorn University Faculty of Pharmaceutical Sciences. 20160310 read0187881/presentations/22486325 扇田 隆司 上川 翼 籾山 京子 林 直樹 Kentaro Kogure 小暮 健太朗 細菌?型分泌機構の解明を目指したエフェクター分泌の定量評価. 日本薬学会第136年会(横浜) 20160300 read0187881/presentations/22486324 M Hasan A Nishimoto T Ohgita S Hama H Kashida H Asanuma Kentaro Kogure Faint electric treatment enhances cellular uptake and intracellular delivery into cytoplasm. 日本薬学会第136年会(横浜) 20160300 read0187881/presentations/22486364 Kentaro Kogure 小暮 健太朗 アスタキサンチンとトコトリエノールの抗酸化併用効果 アスタリールシンポジウム2016 20160213 read0187881/presentations/22486328 Natsumi Shibuya 渋谷 菜摘 亀崎 ちひろ 中島 愛美 石橋 博 ? 進 細井 信造 山下 栄次 Kentaro Kogure 小暮 健太朗 OHラジカル細胞傷害へのアスタキサンチン/トコトリエノールリポソームの抑制効果 第28回ビタミンE研究会(東京) 20160119 read0187881/presentations/22486327 Misuzu Ishikawa 石川 みすず 亀崎 ちひろ 中島 愛美 石橋 博 ? 進 細井 信造 山下 栄次 Kentaro Kogure 小暮 健太朗 α-トコトリエノールとアスタキサンチンのリポソーム膜中における相乗効果の検討. 第28回ビタミンE研究会(東京) 20160119 read0187881/presentations/22486326 濱 進 中島 采香 西 貴之 Kenji Fukuzawa 福澤 健治 Hiroyuki Saito 斎藤 博幸 Kentaro Kogure 小暮 健太朗 薬剤耐性癌の克服を目指したトコフェロールコハク酸ナノベシクルの開発 第28回ビタミンE研究会(東京) 20160119 read0187881/presentations/22486244 Kenji Fukuzawa Kentaro Kogure Sachie Manabe Akira Tokumura In vitro cytotoxicity of alpha-tocopherol succinate, malonate and oxalate on cancer cells and their in vivo anti-cancer effects on mouse melanoma XIII the Biennial Meeting of the Society for Free Radical Research International 20060800 read0187881/presentations/22486365 Kenji Fukuzawa 福澤 健治 Kentaro Kogure 小暮 健太朗 真鍋 幸恵 Akira Tokumura 村 彰 α-トコフェロールジカルボン酸誘導体の抗ガン作用 第17回ビタミンE研究会 20060100 read0187881/presentations/22486245 Kenji Fukuzawa Kentaro Kogure S. Manabe Ichiro Suzuki Akira Tokumura Selective cytotoxicity to cancer cells of α-tocopheryl succinate analogues in vitro and their anti-cancer effects on mouse melanoma in vivo International Interdisciplinary Conference on Vitamins, Coenzymes, and Biofactors 2005 20051100 read0187881/presentations/22486366 Kenji Fukuzawa 福澤 健治 Kentaro Kogure 小暮 健太朗 Akira Tokumura 村 彰 α-トコフェロール・コハク酸の酸化的アポトーシスと抗ガン作用 第5回AOB(Antioxidant Biofactor)研究会:食品機能と酸化ストレス 20050600 read0187881/presentations/22486329 Kenji Fukuzawa 福澤 健治 真鍋 幸恵 Kentaro Kogure 小暮 健太朗 Akira Tokumura 村 彰 マウスに移植した黒色腫瘍に対するα-トコフェロール誘導体の抗ガン効果 日本ビタミン学会大会 20050500 read0187881/presentations/22486367 Kenji Fukuzawa 福澤 健治 真鍋 幸恵 Kentaro Kogure 小暮 健太朗 Akira Tokumura 村 彰 α-トコフェロール誘導体のアポトーシス誘導作用:構造-活性相関 第16回ビタミンE研究会 20050100 read0187881/presentations/22486368 Kenji Fukuzawa 福澤 健治 Kentaro Kogure 小暮 健太朗 中島 佐和 土江 明子 Akira Tokumura 村 彰 ビタミンEによるリン脂質酸化二次産物誘導血管平滑筋細胞アポトーシスの抑制 第15回ビタミンE研究会 20040100 read0187881/presentations/22486330 Kenji Fukuzawa 福澤 健治 赤井 かおり 土屋 浩一郎 Kentaro Kogure 小暮 健太朗 Akira Tokumura 村 彰 玉置 俊晃 配位構造の異なる3つのタイプのFe-NTA錯体による脂質過酸化の誘導 日本過酸化脂質フリーラジカル学会27回大会 20031000 read0187881/presentations/22486369 Kenji Fukuzawa 福澤 健治 中島 佐和 土江 明子 Kentaro Kogure 小暮 健太朗 Akira Tokumura 村 彰 PAF様リン脂質酸化二次産物とビタミンE 第300回脂溶性ビタミン総合研究委員会 20030600 read0187881/presentations/22486332 Akira Tokumura b ێR Cj N, V Kentaro Kogure Kenji Fukuzawa 正常および異常妊娠時のヒト血清リゾホスホリパーゼD活性 第45回日本脂質生化学会 20030600 read0187881/presentations/22486331 赤井 かおり 土屋 浩一郎 Kentaro Kogure 小暮 健太朗 Akira Tokumura 村 彰 玉置 俊晃 Kenji Fukuzawa 福澤 健治 発癌性鉄錯体Fe3+-ニトリロ三酢酸(Fe3+-NTA)の光およびphに依存した活性酵素の産出と脂質過酸化の誘導 第25回日本フリーラジカル学会 20030600 read0187881/presentations/22486333 真鍋 幸恵 Kentaro Kogure 小暮 健太朗 浜 進 森田 健之 Akira Tokumura 村 彰 Kenji Fukuzawa 福澤 健治 ビタミンEコハク酸のガン細胞におけるアポトーシス誘導作用へのH2O2の関与 日本ビタミン学会55回大会 20030500 read0187881/presentations/22486336 Kentaro Kogure 小暮 健太朗 濱 進 Satoru GOTO 後藤 了 Akira Tokumura ?村 彰 Kenji Fukuzawa 福澤 健治 Vitamin Eコハク酸はPKCを直接活性化する 日本薬学会第123年会 20030300 read0187881/presentations/22486335 Akira Tokumura b ێR Cj N Kentaro Kogure c F Gz Kenji Fukuzawa 異常妊娠婦人の血清リゾホスホリパーゼD活性:正常妊娠婦人との価との比較 日本薬学会年会 20030300 read0187881/presentations/22486334 蔭山 千恵子 Akira Tokumura ?村 彰 Kentaro Kogure 小暮 健太朗 Kenji Fukuzawa 福澤 健治 土肥 敏博 ヒト唾液に存在するリゾホスホファチジン酸産生酵素リゾホスホリパーゼDの症状 日本薬学会年会 20030300 read0187881/presentations/22486370 真鍋 幸恵 Kentaro Kogure 小暮 健太朗 森田 健之 Akira Tokumura 村 彰 Kenji Fukuzawa 福澤 健治 抗がん剤として製剤化を指向したビタミンEコハク酸ベクシル 第14回ビタミンE研究会 20030100 read0187881/presentations/22486338 土江 明子 Akira Tokumura ?村 彰 Kentaro Kogure 小暮 健太朗 Kenji Fukuzawa 福澤 健治 ヒト血液中で酸化ストレスを受け生成する短鎖ジカルボン酸含有ホスファチジルコリンの定量 第41回日本薬学会・日本病院薬剤師会中国四国支部学術大会 20021100 read0187881/presentations/22486337 齋藤 恭章 赤井 かおり Kentaro Kogure 小暮 健太朗 Akira Tokumura 村 彰 柴田 瑩 Kenji Fukuzawa 福澤 健治 血清アルブミンの膜脂質過酸化抑制作用 第24回生体膜と薬物の相互作用シンポジウム 20021100 read0187881/presentations/22486345 浜 進 Kentaro Kogure 小暮 健太朗 Satoru GOTO 後藤 了 Ichiro Suzuki 鈴木 一郎 Akira Tokumura ?村 彰 Masayuki Shibuya 渋谷 雅之 Kenji Fukuzawa 福澤 健治 トコフェロール-コハク酸によるPKCを介したNO産生促進機構 第41回日本薬学会・日本病院薬剤師会中国四国支部学術大会 20021000 read0187881/presentations/22486344 Kentaro Kogure 小暮 健太朗 浜 進 Akira Tokumura ?村 彰 Kenji Fukuzawa 福澤 健治 ビタミンEコハク酸の作用発現における細胞内シグナル機構の関与 日本生化学会大会 20021000 read0187881/presentations/22486343 蔭山 千恵子 Akira Tokumura ?村 彰 Kentaro Kogure 小暮 健太朗 Kenji Fukuzawa 福澤 健治 土肥 敏博 ヒト唾液中でリゾホスファチジン酸を産出するリゾホスホリパーゼDの性質 日本生化学会大会 20021000 read0187881/presentations/22486342 中島 佐和 Kentaro Kogure 小暮 健太朗 土江 明子 Akira Tokumura 村 彰 Kenji Fukuzawa 福澤 健治 酸化リン脂質によって誘導される血管平滑筋細胞の障害 日本生化学会大会 20021000 read0187881/presentations/22486341 Akira Tokumura pi J Dq xi q N Kentaro Kogure c F Kenji Fukuzawa 生理活性リン脂質LPAを産出する血漿リゾホスホリパーゼDの性状 日本生化学会大会 20021000 read0187881/presentations/22486340 Kentaro Kogure 小暮 健太朗 木崎 真弓 Ichiro Suzuki 鈴木 一郎 Akira Tokumura ?村 彰 渋谷 雅之 Kenji Fukuzawa 福澤 健治 アポトーシス誘導作用におけるトコフェロールコハク酸類縁体の構造活性相関の検討 日本過酸化脂質フリーラジカル学会26回大会 20021000 read0187881/presentations/22486339 Kentaro Kogure 小暮 健太朗 森田 健之 Akira Tokumura 村 彰 Kenji Fukuzawa 福澤 健治 トコフェロールコハク酸のアポトーシス誘導作用と細胞の活性酸素防御能 日本過酸化脂質フリーラジカル学会26回大会 20021000 read0187881/presentations/22486249 Kenji Fukuzawa Y. Saitoh K. Arai Kentaro Kogure Akira Tokumura A. Shibata Antioxidant effect of bovine serum albumin on iron-chelate superoxide-dependent membrane lipid peroxidation 11th Biennial Scientific Meeting of the International Society for Free Radical Research 20020700 read0187881/presentations/22486248 Kentaro Kogure S. Hama S. Manabe M. Kisaki Akira Tokumura Kenji Fukuzawa High toxicity of α-tocopheryl succinate to cancer cells is due to their failed antioxidative deffence system 11th Biennial Scientific Meeting of the International Society for Free Radical Research 20020700 read0187881/presentations/22486247 Akira Tokumura A. Tsuchie Kentaro Kogure Kenji Fukuzawa Cigarette smoking increases plasma level of phosphatidylcholine having an oxidatively shortened fatty acyl group with a carboxyl terminal 11th Biennial Scientific Meeting of the International Society for Free Radical Research 20020700 read0187881/presentations/22486246 Kenji Fukuzawa Kentaro Kogure M. Morita S. Hama Akira Tokumura Enhancement by alpha-tocopheryl succinate of superoxide and nitric oxide generations in cultured cells International Symposium: Reactive Oxygen and Nitrogen Species: Diagnostic,Preventive and Therapeutic Values 20020700 read0187881/presentations/22486346 Akira Tokumura xi q N, V Kentaro Kogure c F Kenji Fukuzawa J Dq pi 生理活性リン脂質LPAを産出するヒト血漿リゾホスホリパーゼDは多機能性ヌクレオチドホスホジエステラーゼautotaxinである 日本脂質生化学研究会第44回研究集会 20020600 read0187881/presentations/22486251 S. Hama Kentaro Kogure S. Manabe Akira Tokumura Kenji Fukuzawa Anti-cancer effect of a-tocopheryl succinate mediated by apoptosis The 9th Korea-Japan joint symposium of drug design and development 20020500 read0187881/presentations/22486250 Kentaro Kogure S. Hama S. Manabe Ichiro Suzuki M. Shibuya Akira Tokumura Kenji Fukuzawa Role of terminal structure of a-tocopheryl esters in nitric oxide production and apoptosis The 9th Korea-Japan joint symposium of drug design and development 20020500 read0187881/presentations/22486348 浜 進 Kentaro Kogure 小暮 健太朗 中島 佐和 真鍋 幸恵 Akira Tokumura 村 彰 Kenji Fukuzawa 福澤 健治 トコフェロールコハク酸によるNO産生促進効果の機構解析 日本ビタミン学会大会 20020400 read0187881/presentations/22486347 真鍋 幸恵 Kentaro Kogure 小暮 健太朗 浜 進 中島 佐和 Akira Tokumura 村 彰 Kenji Fukuzawa 福澤 健治 黒色腫瘍に対するα-トコフェロールコハク酸の抗ガン効果 日本ビタミン学会大会 20020400 read0187881/presentations/22486351 Akira Tokumura Gs, R q N, V Kentaro Kogure Kenji Fukuzawa ホスファチジン酸による好中球O2-産出に対するホスホリパーゼA2阻害剤の作用 日本薬学会年会 20020300 read0187881/presentations/22486350 Kentaro Kogure 小暮 健太朗 浜 進 真鍋 幸恵 Akira Tokumura 村 彰 Kenji Fukuzawa 福澤 健治 アポトーシス誘導作用を有するトコフェロールコハク酸の構造的因子の検討 日本薬学会年会 20020300 read0187881/presentations/22486349 土江 明子 Akira Tokumura ?村 彰 Kentaro Kogure 小暮 健太朗 Kenji Fukuzawa 福澤 健治 喫煙者及び非喫煙者血漿に存在する酸化ホスファチジルコリン分子種組成の比較 日本薬学会年会 20020300 read0187881/presentations/22486372 浜 進 Kentaro Kogure 小暮 健太朗 森田 元喜 中島 佐和 Akira Tokumura 村 彰 Kenji Fukuzawa 福澤 健治 α-トコフェロールコハク酸によるNO産出促進にPKCが関与する 第13回ビタミンE研究会 20020100 read0187881/presentations/22486371 Kentaro Kogure 小暮 健太朗 浜 進 真鍋 幸恵 Akira Tokumura 村 彰 Kenji Fukuzawa 福澤 健治 トコフェロールコハク酸のガン細胞に対するアポトーシス誘導作用の検討 第13回ビタミンE研究会 20020100 read0187881/presentations/22486352 Satoru GOTO 後藤 了 Kentaro Kogure 小暮 健太朗 安部 一豊 Hitoshi Hori 堀 均 Hiroshi Chuman 中馬 寛 赤須 通範 Hiroshi Terada 寺田 弘 Cepharanthineの立体構造と非Phenol型Radical消去活性 第29回構造活性相関シンポジウム 20011100 read0187881/presentations/22486254 Kenji Fukuzawa Kentaro Kogure M. Morita S. Hama S. Nakashima Akira Tokumura Enhancement by α-tocopheryl hemisuccinate of nitric oxide production indeced by lipopolysaccharide and interferon-γthrough up-regulation of protein kinase C in rat vascular smooth muscle cells 2nd China-Japan International Conference on Vitamins 20011000 read0187881/presentations/22486253 Kentaro Kogure M. Morita S. Nakashima S. Hama Akira Tokumura Kenji Fukuzawa Superoxide is responsible for apoptosis in rat vascular smooth muscle cells induced by α-tocopheryl hemisuccinate 2nd China-Japan International Conference on Vitamins 20011000 read0187881/presentations/22486252 K. Tominaga Kentaro Kogure 小暮 健太朗 Kenji Fukuzawa 福澤 健治 Akira Tokumura 村 彰 Property of secretory phospholipase D which generates bioactive lysophosphatidic acid in guinea-pig peritoneal washing The 1st Takeda Science Foundation Symposium on Pharma Sciences:Lipids in Signaling and Related Diseases 20011000 read0187881/presentations/22486353 Satoru GOTO 後藤 了 Kentaro Kogure 小暮 健太朗 Hiroshi Chuman 中馬 寛 赤須 通範 Hiroshi Terada 寺田 弘 柔らかいCepharanthineと堅いTetrandrineのラジカル消去活性 日本薬学会第121年会 20010300 read0187881/presentations/22486355 Satoru GOTO 後藤 了 Kentaro Kogure 小暮 健太朗 Hiroshi Chuman 中馬 寛 赤須 通範 Hiroshi Terada 寺田 弘 セファランチンノラジカル消去活性と化学構造のゆらぎ 日本薬学会120年会 20000300 read0187881/presentations/22486354 西村 美紀 Kentaro Kogure 小暮 健太朗 Satoru GOTO 後藤 了 Hiroshi Terada 寺田 弘 Capsaicinの特徴的な抗酸化作用機構 日本薬学会第120年会 20000300 read0187881/presentations/22486356 西村 美紀 Kentaro Kogure 小暮 健太朗 Satoru GOTO 後藤 了 Hiroshi Terada 寺田 弘 Capsaicinの特徴的な抗酸化作用への膜物性変化の関与 第38回日本薬学会・日本病院薬剤師会中国四国支部学術大会 19991000 read0187881/presentations/22486357 西村 美紀 Kentaro Kogure 小暮 健太朗 Satoru GOTO 後藤 了 Hiroshi Terada 寺田 弘 辛味成分Capsaicinの膜系における抗酸化活性について 第72回日本生化学会 19990900 read0187881/presentations/22486359 Satoru GOTO 後藤 了 Kentaro Kogure 小暮 健太朗 Hiroshi Chuman 中馬 寛 Hiroshi Terada 寺田 弘 赤須 通範 大環状 tetrahydroisoquinoline誘導体の構造とラジカル消去活性の相関 日本薬学会第119大会 19990300 read0187881/presentations/22486358 西村 美紀 Kentaro Kogure 小暮 健太朗 Satoru GOTO 後藤 了 Hiroshi Terada 寺田 弘 辛味成分Capsaicinの抗酸化作用機構 日本薬学会119年会 19990300 read0187881/presentations/22486360 Satoru GOTO 後藤 了 Kentaro Kogure 小暮 健太朗 安部 一豊 赤須 通範 Hiroshi Terada 寺田 弘 大環状Bis-benzylisoquinoline誘導体の動的構造の解析とラジカル消去活性 第26回構造活性相関シンポジウム 19981100 read0187881/presentations/22486361 Satoru GOTO 後藤 了 安部 一豊 Kentaro Kogure 小暮 健太朗 赤須 通範 Hiroshi Terada 寺田 弘 Cepharanthineおよびその誘導体のラジカル消去活性発現における動的構造の影響 第24回アルカロイド討論会 19980600 read0187881/books_etc/12782009 濱 進 板倉 祥子 Kentaro Kogure 小暮 健太朗 がん微小環境をターゲットとしたDDS技術開発 Technical Information Institute Co., Ltd. 株式会社 技術情報協会 20170500 read0187881/books_etc/12782010 眞岡 孝至 Kentaro Kogure 小暮 健太朗 第22章 アスタキサンチン 20160000 read0187881/books_etc/12782011 Satoru GOTO 後藤 了 Kentaro Kogure 小暮 健太朗 Koichiro Tsuchiya 土屋 浩一郎 尾関 哲也 エピソード物理化学 20110311 read0187881/books_etc/12782012 Kenji Fukuzawa 福澤 健治 Kentaro Kogure 小暮 健太朗 真鍋 幸恵 Akira Tokumura 村 彰 ビタミンE研究の進歩 XII KYORITSU SHUPPAN.CO.,LTD 共立出版株式会社 20070000 read0187881/books_etc/12782013 真鍋 幸恵 Kentaro Kogure 小暮 健太朗 森田 健之 Akira Tokumura 村 彰 Kenji Fukuzawa 福澤 健治 ビタミンE研究の進歩 XI KYORITSU SHUPPAN.CO.,LTD 共立出版株式会社 20040000 read0187881/books_etc/12782014 Kentaro Kogure 小暮 健太朗 Akira Tokumura 村 彰 Kenji Fukuzawa 福澤 健治 ビタミンE研究の進歩 X KYORITSU SHUPPAN.CO.,LTD 共立出版株式会社 20020000 小暮 健太朗 日本薬学会北海道支部奨励賞 KOGURE Kentaro 小暮 健太朗 The 1st FIP-APSTJ Joint workshop on Gene Delivery``The Best Presentation Award 小暮 健太朗 第一回上村修三郎「がん研究」奨励賞