! Fuminori Tanihara, Maki Hirata, Satoru Iizuka, Shinya Sairiki, Masahiro Nii, Nhien Thi Nguyen, Quynh Anh Le, Takayuki Hirano and Takeshige Otoi : Relationship among ovarian follicular status, developmental competence of oocytes, and anti-M llerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts., Animal Science Journal, Vol.90, No.6, 712-718, 2019. Fuminori Tanihara, Maki Hirata, Morikawa Shigeki, Thi Nhien Nguyen, Le Anh Quynh, Hirano Takayuki, Fukumi Yoshiyuki, Abe Toshiaki and Takeshige Otoi : The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts, The Journal of Reproduction and Development, Vol.65, No.5, 475-479, 2019. Namula Zhao, Manita Wittayarat, Maki Hirata, Hirano Takayuki, Nguyen Thi Nhien, Le Anh Quynh, Fahrudin Mokhamad, Fuminori Tanihara and Takeshige Otoi : Genome mutation after the introduction of the gene editing by electroporation of Cas9 protein (GEEP) system into bovine putative zygotes, In Vitro Cellular & Developmental Biology. Animal, Vol.55, No.8, 598-603, 2019. Maki Hirata, Manita Wittayarat, Takayuki Hirano, Thi Nhien Nguyen, Le Anh Quynh, Zhao Namula, Mokhamad Fahrudin, Fuminori Tanihara and Takeshige Otoi : The relationship between embryonic development and the efficiency of target mutations in porcine endogenous retroviruses (PERVs) pol genes in porcine embryos., Animals : An Open Access Journal from MDPI, Vol.9, No.9, E593, 2019. A Quynh Le, Maki Hirata, T Nhien Nguyen, Koki Takebayashi, Manita Wittayarat, Yoko Sato, Zhao Namula, Masahiro Nii, Fuminori Tanihara and Takeshige Otoi : Effects of electroporation treatment using different concentrations of Cas9 protein with gRNA targeting Myostatin (MSTN) genes on the development and gene editing of porcine zygotes., Animal Science Journal, Vol.91, No.1, e13386, 2020. Namula Zhao, Yoko Sato, Manita Wittayarat, Quynh Le Anh, Thi Nhien Nguyen, Qingyi Lin, Maki Hirata, Fuminori Tanihara and Takeshige Otoi : Curcumin supplementation in maturation medium improves the maturation, fertilisation, and developmental competence of porcine oocytes., Acta Veterinaria Hungarica, Vol.68, No.3, 298-304, 2020. Yoko Sato, Ryota Kuriwaki, Shiki Hagino, Megumi Shimazaki, Rentsenkhand Sambuu, Maki Hirata, Fuminori Tanihara, Mitsuhiro Takagi, Masayasu Taniguchi and Takeshige Otoi : Abnormal functions of Leydig cells in crossbred cattle-yak showing infertility., Reproduction in Domestic Animals = Zuchthygiene, Vol.55, No.2, 209-216, 2020. T N Nguyen, Maki Hirata, Fuminori Tanihara, Y Sato, Z Namula, Le Trong Quang, M Wittayarat, M Fahrudin and Takeshige Otoi : In vitro Development of Zona Pellucida-free Porcine Zygotes Cultured Individually after Vitrification., Cryo Letters, Vol.41, No.2, 86-91, 2020. Fuminori Tanihara, Maki Hirata, Nhien Nguyen Thi, Le Anh Quynh, Takayuki Hirano and Takeshige Otoi : Generation of viable PDX1 gene-edited founder pigs as providers of nonmosaics., Molecular Reproduction and Development, Vol.87, No.4, 471-481, 2020. Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi : Assessment of PDX-1-deficient pigs generated using the CRISPR/Cas9 system introduced into porcine zygotes via electroporation, The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019. Le Anh Quynh, Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Hirano Takayuki and Takeshige Otoi : Effect of Cas9 protein levels on genomic mutations using the gene editing by electroporation of Cas9 protein (GEEP) system in putative zygotes, The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019. Nguyen Thi Nhien, Fuminori Tanihara, Maki Hirata, Hirano Takayuki, Le Anh Quynh and Takeshige Otoi : Efficiency of gene editing by electroporation of Cas9 protein (GEEP) to generate GGTA1-modified pigs, The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019. Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi : Effects of CRISPR/Cas9-mediated gene targeting of porcine endogenous retrovirus on the developmental competence of porcine embryos, The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019. 谷原 史倫, 平田 真樹, Nguyen Thi Nhien, Quynh Anh Le, 平野 隆之, 音井 威重 : エレクトロポレーションを用いたCRISPR/Cas9システムのブタ体外受精卵への導入によるCD163遺伝子改変ブタの作製, 第162回日本獣医学会学術集会, 2019年9月. 平田 真樹, 谷原 史倫, NGUYEN THI NHIEN, LE ANH QUYNH, 平野 隆之, 音井 威重 : ブタ体外受精卵におけるCRISPR/Cas9システムを用いた複数遺伝子の同時改変, 第7回日本先進医工学ブタ研究会, 2019年10月. 音井 威重, 平田 真樹, NGUYEN THI NHIEN, LE ANH QUYNH, 平野 隆之, 谷原 史倫 : GEEP法を用いた遺伝子改変ブタの作製と遺伝子改変効率, 第7回日本先進医工学ブタ研究会, 2019年10月. 谷原 史倫, 平田 真樹, NGUYEN THI NHIEN, LE ANH QUYNH, 平野 隆之, 音井 威重 : CRISPR/Cas9システムによるブタ体外受精卵のINS遺伝子への点変異導入, 第7回日本先進医工学ブタ研究会, 2019年10月. Takayuki Hirano, Maki Hirata, Shigeyuki Fujimoto, Thi Nhien Nguyen, Le Anh Quynh, Fuminori Tanihara and Takeshige Otoi : Comparative analysis of bilirubin glucuronidation activity in 2D- and 3D-cultured human hepatocellular carcinoma HepG2 cells., In Vitro Cellular & Developmental Biology. Animal, Vol.56, No.4, 277-280, 2020. Maki Hirata, Manita Wittayarat, Zhao Namula, Anh Quynh Le, Qingyi Lin, Thi Nhien Nguyen, Koki Takebayashi, Yoko Sato, Fuminori Tanihara and Takeshige Otoi : Evaluation of multiple gene targeting in porcine embryos by the CRISPR/Cas9 system using electroporation., Molecular Biology Reports, Vol.47, No.7, 5073-5079, 2020. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Osamu Sawamoto, Takeshi Kikuchi, Masako Doi and Takeshige Otoi : Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes., BMC Biotechnology, Vol.20, No.1, 40, 2020. Maki Hirata, Manita Wittayarat, Fuminori Tanihara, Yoko Sato, Zhao Namula, Anh Quynh Le, Qingyi Lin, Koki Takebayashi and Takeshige Otoi : One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs., In Vitro Cellular & Developmental Biology. Animal, Vol.56, No.8, 614-621, 2020. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Le Anh Quynh, Manita Wittayarat, Mokhamad Fahrudin, Takayuki Hirano and Takeshige Otoi : Generation of CD163-edited pig via electroporation of the CRISPR/Cas9 system into porcine in vitro-fertilized zygotes., Animal Biotechnology, Vol.32, 147-154, 2021. Manita Wittayarat, Maki Hirata, Zhao Namula, Yoko Sato, T Nhien Nguyen, A Quynh Le, Qingyi Lin, Koki Takebayashi, Fuminori Tanihara and Takeshige Otoi : Introduction of a point mutation in the KRAS gene of in vitro fertilized porcine zygotes via electroporation of the CRISPR/Cas9 system with single-stranded oligodeoxynucleotides., Animal Science Journal, Vol.92, No.1, e13534, 2021. Nguyen Nhien Thi, Wittayarat Manita, Zhao Namula, Sato Yoko, Anh Le Quynh, Lin Qingyi, Takebayashi Koki, Fuminori Tanihara, Maki Hirata and Takeshige Otoi : Chlorogenic acid and insulin-transferrin-selenium supplementation during in vitro maturation enhances the developmental competence of interspecies chimera blastocysts following cell injection., Journal of Applied Animal Research, Vol.49, No.1, 486-491, 2021. Kim Thi Lanh Do, Manita Wittayarat, Yoko Sato, Kaywalee Chatdarong, Theerawat Tharasanit, Mongkol Techakumphu, Maki Hirata, Fuminori Tanihara, Masayasu Taniguchi and Takeshige Otoi : Comparison of blastocyst development between cat-cow and cat-pig interspecies somatic cell nuclear transfer embryos under the treatment of Trichostatin A., Biology bulletin of the Russian Academy of Sciences, Vol.48, 107-117, 2021. Anh Quynh Le, Fuminori Tanihara, Manita Wittayarat, Zhao Namula, Yoko Sato, Qingyi Lin, Koki Takebayashi, Maki Hirata and Takeshige Otoi : Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages., BMC Research Notes, Vol.14, No.1, 7, 2021. Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Le Anh, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Fuminori Tanihara and Takeshige Otoi : Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos., Animals : An Open Access Journal from MDPI, Vol.11, No.2, 2021. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Osamu Sawamoto, Takeshi Kikuchi and Takeshige Otoi : One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis., International Journal of Molecular Sciences, Vol.22, No.5, 2249, 2021. 平田 真樹 : 畜産領域におけるアニマルウェルフェアとOne health, 第23回臨床腸内微生物学会総会・学術集会, 2020年9月. 吉田 知加, 平田 真樹, 松岡 美樹, 大貫 燿, 岡 健太郎, 高橋 志達, 原口 雅宣, 森松 文毅 : 照明色の違いが豚の攻撃的行動と生産性に及ぼす影響, LED総合フォーラム2021in徳島, P-19, 2021年2月. 谷原 史倫, 平田 真樹, 音井 威重, 澤本 修, 菊地 健志 : GEEP法によるGGTA1遺伝子およびCMAH遺伝子の同時改変ブタの作出, 第24回日本異種移植研究会, 2021年2月.