Tokushima University / Educator and Researcher Directory --- Nagamune, Hideaki

Personal Web Page

https://researchmap.jp/nagamunehideaki (researchmap)

Field of Study

○	Microbiology, Immunology

Subject of Study

○	QSAR and virulence analyses of streptococcal toxins, Application of bacterial toxins to the immunotherapy agaist cancer and the development of cellular medicines, Study on the substances stimulating the immune system by their action to biological membranes (bacterial toxins, Streptococci, immunotherapy, immunostimulater)

Book / Paper

Book:

1.	Hitoshi Matsuki, Kumiko Irisuna, Yayoi Sakamoto, Hideaki Nagamune and Shoji Kaneshina : 高圧力下の生物科学, さんえい出版株式会社, Kyoto, Nov. 2006. (Summary) 細胞工学的な手法を用いてフルオレセインイソチオシアネート特異的モノクローナル抗体を作製し，フルオレセインとの抗原抗体反応を蛍光分光法で追跡した．常圧下における熱力学量の結果から，温度は複合体から両者の解離を促進し，両者の会合反応は低温ではエントロピー駆動であり，高温ではエンタルピー駆動であることを示した．さらに両者の反応に対する圧力の効果を調べ，圧力も両者の解離を促進し，圧力と温度は相乗的に作用することを明確にした．
2.	Hideaki Nagamune : For the concentration of proteins, 株式会社廣川書店, Tokyo, Jun. 2001.
3.	石田寿昌, 石野良純, Hideaki Nagamune, 岡本敬の介, 梶本和昭, 加藤郁之進, 金保安則, 河野通明, 小池晴彦, 佐藤真悟, 鮫島勇次, Yasuo Shinohara, Akihiko Tsuji, Hiroshi Terada, 富田基郎, 友尾幸司, 野沢義則, 野元裕, 濱崎直孝, 日野真美, 平松宏一, 藤井敏, 藤井儀夫, 洪英満, Yoshiko Matsuda, 森秀治, 山崎尚志, 山本格 and 若原章男 : タンパク質と核酸の分離精製, 株式会社廣川書店, Tokyo, Jun. 2001.
4.	Yoshiko Matsuda, Akihiko Tsuji and Hideaki Nagamune : タンパク質と核酸の分離精製·基礎と実験(共著), 株式会社廣川書店, Tokyo, Jan. 2001. (Summary) 本著作はタンパク質と核酸の精製方法を解析したもので，松田·辻は細胞分画，蛋白質抽出法と精製法および核酸，DNA, plasmid, mRNAの分離，精製法について解説した．また長宗は，微量の生理活性タンパク質を調製して使用する際に必須である濃縮技術の中でも特に利用頻度の高い7種類の方法:塩析法，有機溶媒沈殿法，等電点沈殿法，脱水法，限外濾過法，凍結乾燥法，遠心真空蒸発法を例にとり，その原理や具体的な操作方法について解説を行った．
5.	Hideaki Nagamune, Kazuaki Muramatsu, Masakatsu Higashine, Tetsuya Akamatsu, Yasuhiro Tamai, Yasuo Yonetomi, Akihiko Tsuji, Keisuke Izumi, Takemasa Sakaguchi, Tetsuya Yoshida and Yoshiko Matsuda : Cyclic AMP-inducible procalcitonin processing in thyroidal parafollicular cells is regulated by the Kexin family protease, PC1., IOS Press, Amsterdam, Mar. 1997. (Summary) カルシウム調節ホルモンであるカルシトニンは甲状腺傍濾胞細胞より分泌されるが, 不活性な前駆体として合成された後, プロセシングを受けて初めて活性化される. 本研究ではcAMP刺激により著増するカルシトニンのプロセシングがKexin family proteaseに属する酵素PC1により調節されることを初めて明らかにした. (Keyword) Calcitonin / Processing / PC1
6.	Katsuhiko Hirota, Fusao Ohta, Hideaki Nagamune, Sonia Senesi, Giovanna Batoni, Giorgio Freer, Terushige Kawata and Komei Fukui : A comparison of various EIA plates for the analysis of polysaccharide antigens and serological identification of oral streptococci , New Perspectives on Streptococcal Infections Zentralblatt fur Bakteriologie Supplement 22, Gustav Fischer, U.K., Jan. 1992.
7.	Hideaki Nagamune, Fusao Ohta, Sonia Senesi, Giovanna Batoni and Komei Fukui : Monoclonal antibodies specific for a polysaccharide antigenic determinant of Streptococcus mutans (serotypes c, e and f) , New Perspectives on Streptococcal Infections Zentralblatt fur Bakteriologie Supplement 22, Gustav Fischer, U.K., Jan. 1992.
8.	Hideaki Nagamune and Hiroshi Terada : 単クローン抗体調製とキャラクタリゼーション, 株式会社廣川書店, Tokyo, Dec. 1990. (Summary) 研究用試薬としてだけでなく医学臨床診断や治療用にも広く応用されている単クローン抗体について，本書ではその原理から解きほぐし，詳細な免疫方法やハイブリドーマの調製方法，また様々な抗体の応用方法について詳細に解説した．巻末には解説した内容についての理解が確認できるように例題を示し，初めて単クローン抗体を利用しようとする研究者やその原理を理解しようとする学生にとって，マニュアルとしても参考書としても使える入門書にまとめた．
9.	阿久津秀雄, 伊藤明夫, Hideaki Nagamune, 久野健夫, 小南英紀, 田代裕, 田中啓二, Akihiko Tsuji, Hiroshi Terada, 中野明彦, 中安博司, 能見貴人, 日野幸伸, 二井将光, 前田正知, Yoshiko Matsuda, 三原勝芳, 克子山下 and 山本章嗣 : 膜酵素I –基礎と実験-，膜酵素II –基礎と実験-, 株式会社廣川書店, Tokyo, May 1990. (Summary) 本著作は膜酵素の精製や解析法を詳細に解説したものである．第7章では，膜酵素の膜結合様式，界面活性剤の性質，膜酵素の可溶化，精製，純度検定法について解説した．また，第8章(pp.208-239)においては，生物の神経伝達や原尿の再吸収において重要な役割を果たす細胞膜酵素Na,K-ATPaseの触媒サブユニットの構造解析を具体例に取り，蛋白質一次構造をコードするcDNA構造の解析手法を分かりやすく解説した．その際に必要となるDNAやRNAの取り扱い技術，クローニング技術なども，初心者にも分かりやすい表形式にして巻末(第二巻末: pp455-491)に紹介している．
10.	市川哲生, Fusao Ota, Hideaki Nagamune, 久保兼信, 桜林郁之介, 佐藤真悟, Yasuo Shinohara, 高木俊夫, 竹尾和典, 田中経彦, Akihiko Tsuji, Hiroshi Terada, 中尾真, 中村和行, 藤田清貴, Yoshiko Matsuda and 真鍋敬 : 電気泳動法 –基礎と実験-, 株式会社廣川書店, Tokyo, Apr. 1989. (Summary) 本著作は，タンパク質の電気泳動法の代表例を挙げてその原理や具体的実施法を詳細に解説している．辻らは等電点電気泳動の原理，方法，応用例を解説した．長宗らは，第7章(pp.255-305)においてゲル電気泳動後にゲル中で蛋白質を検出したり，泳動後の蛋白質を転写した膜上で特定の分子だけを検出する場合に汎用される方法の原理と具体的操作法を分かりやすく解説し，第9章(pp.329-344)では，泳動後のゲルの保存方法やゲル中からの蛋白質の回収方法を詳しく述べた．また第11章(pp.371-410)においては，電気泳動に用いられる各種試薬や標準蛋白質の性状について詳述するともに蛋白質の検出法や染色法の各論について，分かりやすい表形式で紹介した．
11.	Hideaki Nagamune, Osamu Urayama, Yukichi Hara and Makoto Nakao : Ouabain-sensitive, K+-dependent and K+-independent phosphatase activities in purified pig kidney Na+, K+-ATPase , Energy Transduction in ATPases, Yamada Science Foundation, Osaka, Jan. 1988.
12.	Makoto Nakao, Osamu Urayama, Hideaki Nagamune, Toshiko Nakao, Kanako Sato, Fumiko Nagai and Itsu Kano : Monoclonal antibodies against kidney Na+, K+-ATPase , Perspectives of Biological Energy Transduction, Academic Press, U.S.A., Jan. 1987.

Academic Paper (Judged Full Paper):

1.	Takuya YAMADA, Yugo YAMAMORI, Nanami Matsuda, Hideaki Nagamune, Kazuto Ohkura, Toshifumi Tomoyasu and Atsushi Tabata : Streptolysin S induces pronounced calcium-ion influx-dependent expression of immediate early genes encoding transcription factors, Scientific Reports, Vol.13, No.13720, 2023. (Summary) Anginosus group streptococci (AGS) are opportunistic human pathogens of the oral cavity. The β-hemolytic subgroup of Streptococcus anginosus subsp. anginosus secretes streptolysin S (SLS) and exhibits not only hemolytic activity but also cytotoxicity toward cultured human cell lines. However, the detailed mechanism of action of SLS and the cellular responses of host cells have not yet been fully clarified. To determine the pathogenic potential of SLS-producing β-hemolytic S. anginosus subsp. anginosus, the SLS-dependent response induced in the human oral squamous cell carcinoma HSC-2 cells was investigated to determine the pathogenic potential of SLS-producing β-hemolytic S. anginosus subsp. anginosus. This study revealed that the Ca influx and the expression of immediate early genes (IEGs) encoding transcription factors such as early growth responses (EGRs) and activator protein-1 (AP-1) were greatly increased in HSC-2 cells incubated with the culture supernatant of SLS-producing β-hemolytic S. anginosus subsp. anginosus. Moreover, this SLS-dependent increase in expression was significantly suppressed by Ca chelation, except for jun. These results suggest that SLS caused Ca influx into the cells following greatly enhanced expression of IEG-encoding transcription factors. The results of this study may help in understanding the pathogenicity of SLS-producing AGS. (Keyword) cytolysin / Streptococcus (Link to Publication Site) ● Publication site (DOI): 10.1038/s41598-023-40981-1 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37608082 ● Search Scopus @ Elsevier (PMID): 37608082 ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-023-40981-1 (DOI: 10.1038/s41598-023-40981-1, PubMed: 37608082)
2.	Toshifumi Tomoyasu, Airi Matsumoto, Ayuko Takao, Atsushi Tabata and Hideaki Nagamune : A simple method to differentiate three classes of cholesterol-dependent cytolysins., Journal of Microbiological Methods, Vol.207, 2023. (Summary) Cholesterol-dependent cytolysins (CDCs) are proteinaceous toxins widely distributed in gram-positive pathogenic bacteria. CDCs can be classified into three groups (I-III) based on the mode of receptor recognition. Group I CDCs recognize cholesterol as their receptor. Group II CDC specifically recognizes human CD59 as the primary receptor on the cell membrane. Only intermedilysin from Streptococcus intermedius has been reported as a group II CDC. Group III CDCs recognize both human CD59 and cholesterol as receptors. CD59 contains five disulfide bridges in its tertiary structure. Therefore, we treated human erythrocytes with dithiothreitol (DTT) to inactivate CD59 on membranes. Our data showed that DTT treatment caused a complete loss of recognition of intermedilysin and an anti-human CD59 monoclonal antibody. In contrast, this treatment did not affect the recognition of group I CDCs, judging from the fact that DTT-treated erythrocytes were lysed with the same efficiency as mock-treated human erythrocytes. The recognition of group III CDCs toward DTT-treated erythrocytes was partially reduced, and these results are likely due to the loss of human CD59 recognition. Therefore, the degree of human CD59 and cholesterol requirements of uncharacterized group III CDCs frequently found in Mitis group streptococci can be easily estimated by comparing the amounts of hemolysis between DTT-treated and mock-treated erythrocytes. (Keyword) bacterial toxins / Cytotoxins / Cell Membrane / Erythrocytes / cholesterol (Link to Publication Site) ● Publication site (DOI): 10.1016/j.mimet.2023.106696 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36898586 ● Search Scopus @ Elsevier (PMID): 36898586 ● Search Scopus @ Elsevier (DOI): 10.1016/j.mimet.2023.106696 (DOI: 10.1016/j.mimet.2023.106696, PubMed: 36898586)
3.	Shuto Yokohata, Kazuto Ohkura, Hideaki Nagamune, Toshifumi Tomoyasu and Atsushi Tabata : Human serum albumin stabilizes streptolysin S activity secreted in the extracellular milieu by streptolysin S-producing streptococci., Microbiology and Immunology, Vol.67, No.2, 58-68, 2022. (Summary) Anginosus group streptococci (AGS) are opportunistic pathogens of the human oral cavity; however, their pathogenicity has not been discussed in detail. Oral streptococci live in the gingival sulcus, from where they can easily translocate into the bloodstream due to periodontal diseases and dental treatment and cause hazardous effects on the host through their virulence factors. Streptolysin S (SLS), a pathogenic factor produced by β-hemolytic species/strains belonging to AGS, plays an important role in damaging host cells. Therefore, we investigated the SLS-dependent cytotoxicity of β-hemolytic Streptococcus anginosus subsp. anginosus (SAA), focusing on different growth conditions such as in the bloodstream. Consequently, SLS-dependent hemolytic activity/cytotoxicity in the culture supernatant of β-hemolytic SAA was stabilized by blood components, particularly human serum albumin (HSA). The present study suggests that the secreted SLS, not only from β-hemolytic SAA, but also from other SLS-producing streptococci, is stabilized by HSA. As HSA is the most abundant protein in human plasma, the results of this study provide new insights into the risk of SLS-producing streptococci which can translocate into the bloodstream. (Keyword) Humans / Streptolysins / Serum Albumin, Human / Streptococcus pyogenes / Virulence / Virulence Factors / Bacterial Proteins (Link to Publication Site) ● Publication site (DOI): 10.1111/1348-0421.13042 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36478453 ● Search Scopus @ Elsevier (PMID): 36478453 ● Search Scopus @ Elsevier (DOI): 10.1111/1348-0421.13042 (DOI: 10.1111/1348-0421.13042, PubMed: 36478453)
4.	Atsushi Tabata, Airi Matsumoto, Ai Fujimoto, Kazuto Ohkura, Takuya Ikeda, Hiroki Oda, Shuto Yokohata, Miho Kobayashi, Toshifumi Tomoyasu, Ayuko Takao, Hisashi Ohkuni and Hideaki Nagamune : Dual functions of discoidinolysin, a cholesterol-dependent cytolysin with N-terminal discoidin domain produced from strain Nm-76., Journal of Oral Microbiology, Vol.14, No.1, 2105013, 2022. (Summary) Some strains of exhibit β-hemolysis due to the β-hemolytic activity of cholesterol-dependent cytolysin (CDC). Recently, a gene encoding an atypical lectinolysin-related CDC was found in strain Nm-76. However, the product of this gene remains uncharacterized. We aimed to characterize this atypical CDC and its molecular functions and contribution to the pathogenicity of strain Nm-76. Phylogenetic analysis of the CDC gene was conducted based on the web-deposited information. The molecular characteristics of CDC were investigated using a gene-deletion mutant strain and recombinant proteins expressed in . The gene encoding CDC found in Nm-76 and its homolog are distributed among many strains. This CDC is phylogenetically different from other previously characterized CDCs, such as -derived human platelet aggregation factor (Sm-hPAF)/lectinolysin and mitilysin. Because this CDC possesses an additional N-terminal domain, including a discoidin motif, it was termed discoidinolysin (DLY). In addition to the preferential lysis of human cells, DLY displayed N-terminal domain-dependent facilitation of human erythrocyte aggregation and intercellular associations between human cells. DLY functions as a hemolysin/cytolysin and erythrocyte aggregation/intercellular association molecule. This dual-function DLY could be an additional virulence factor in . (Link to Publication Site) ● Publication site (DOI): 10.1080/20002297.2022.2105013 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35937899 ● Search Scopus @ Elsevier (PMID): 35937899 ● Search Scopus @ Elsevier (DOI): 10.1080/20002297.2022.2105013 (DOI: 10.1080/20002297.2022.2105013, PubMed: 35937899)
5.	Atsushi Tabata, Rina Shirai, Haruka Miki, Yukihiro Nishikawa, Tatsuya Kashima, Tomomi Aoyama, Shu Murakami, Momoyo Azuma, Toshifumi Tomoyasu and Hideaki Nagamune : Hapten-labeled fusion-polymerase chain reaction of multiple marker genes for the application of immunochromatographic test., Journal of Bioscience and Bioengineering, Vol.134, No.1, 70-76, 2022. (Summary) A variety of methods have been reported using polymerase chain reaction (PCR)-based nucleic acid testing (NAT) because of its potential to be used in highly sensitive inspection systems. Among these NATs, fusion-PCR (also called as overlap-extension-PCR) has been focused on this study and adopted to generate the fused amplicon composed of plural marker gene fragments for detection. Generally, conventional agarose gel electrophoresis followed by gel staining is employed to check the PCR results. However, these are time-consuming processes that use specific equipment. To overcome these disadvantages, the immunochromatographic test (ICT) for the detection of PCR amplicons with hapten-labels that were generated by PCR using hapten-labeled primers was also adopted in this study. Based on these concepts, we constructed the systems of hapten-labeled fusion-PCR (HL-FuPCR) followed by ICT (HL-FuPCR-ICT) for the two and three marker genes derived from pathogenic microbe. As a result, we successfully developed a two marker genes system for the pathogenic influenza A virus and a three marker genes system for the penicillin-resistant Streptococcus pneumoniae. These detection systems of HL-FuPCR-ICT are characterized by simple handling and rapid detection within few minutes, and also showed the results as clear lines. Thus, the HL-FuPCR-ICT system introduced in this study has potential for use as a user-friendly inspection tool with the advantages especially in the detection of specific strains or groups expressing the characteristic phenotype(s) such as antibiotic resistance and/or high pathogenicity even in the same species. (Keyword) DNA Primers / Haptens / Immunologic Tests / Polymerase Chain Reaction / Sensitivity and Specificity (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jbiosc.2022.03.006 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35450786 ● Search Scopus @ Elsevier (PMID): 35450786 ● Search Scopus @ Elsevier (DOI): 10.1016/j.jbiosc.2022.03.006 (DOI: 10.1016/j.jbiosc.2022.03.006, PubMed: 35450786)
6.	Hiroki Nakai, Daiu Akiyama, Yoshiaki Taniguchi, Iori Kishinobu, Hiromichi Wariishi, Yasuhide Ohno, Masao Nagase, Takuya Ikeda, Atsushi Tabata and Hideaki Nagamune : Charge-independent protein adsorption characteristics of epitaxial graphene field-effect transistor on SiC substrate, Journal of Applied Physics, Vol.130, No.7, 074502-1-074502-8, 2021. (Keyword) graphene (Tokushima University Institutional Repository) ● Metadata: 116163 (Link to Publication Site) ● Publication site (DOI): 10.1063/5.0054688 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1063/5.0054688 (Tokushima University Institutional Repository: 116163, DOI: 10.1063/5.0054688)
7.	Keita Hoshi, Kazuma Kusumoto, Airi Matsumoto, Atsushi Tabata, Hideaki Nagamune, Eiji Hase, Takeo Minamikawa, Takeshi Yasui, Yasushi Yoshida, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : Synthesis of D-π-A type benzothiazolepyridinium salt composite and its application as photo-degradation agent for amyloid fibrils, Bioorganic & Medicinal Chemistry Letters, Vol.50, 128324, 2021. (Summary) We have synthesized a cyan fluorescent benzothiazole-pyridinium salt composite based on D-p-A architecture. This salt was found to work as not only a two- and three-photon excitable fluorophore but also a degradation agent against amyloid fibrils under LED irradiation conditions. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bmcl.2021.128324 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34403727 ● Search Scopus @ Elsevier (PMID): 34403727 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bmcl.2021.128324 (DOI: 10.1016/j.bmcl.2021.128324, PubMed: 34403727)
8.	Keita Hoshi, Masami Itaya, Koki Tahara, Airi Matsumoto, Atsushi Tabata, Hideaki Nagamune, Yasushi Yoshida, Eiji Hase, Takeo Minamikawa, Takeshi Yasui, Tetsuro Katayama, Akihiro Furube, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : Two-photon excitable boron complex based on tridentate imidazo[1,5-a]pyridine ligand for heavy- atom-free mitochondria-targeted photodynamic therapy, RSC Advances, Vol.11, 26403-26407, 2021. (Tokushima University Institutional Repository) ● Metadata: 116625 (Link to Publication Site) ● Publication site (DOI): 10.1039/d1ra05059a (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1039/d1ra05059a (Tokushima University Institutional Repository: 116625, DOI: 10.1039/d1ra05059a)
9.	Airi Matsumoto, Atsushi Tabata, Kazuto Ohkura, Hiroki Oda, Chihiro Kodama, Hisashi Ohkuni, Ayuko Takao, Ken Kikuchi, Toshifumi Tomoyasu and Hideaki Nagamune : Molecular characteristics of an adhesion molecule containing cholesterol-dependent cytolysin-motif produced by mitis group streptococci, Microbiology and Immunology, Vol.65, No.2, 61-75, 2021. (Summary) Streptococcus pseudopneumoniae (SPpn) is a relatively new species closely related to S. pneumoniae (SPn) and S. mitis (SM) belonging to the Mitis group of the genus Streptococcus (MGS). Although genes encoding various pneumococcal virulence factors have been observed in the SPpn genome, the pathogenicity of SPpn against human, including the roles of virulence factor candidates, is still unclear. The present study focused on and characterized a candidate virulence factor previously reported in SPpn with deduced multiple functional domains, such as lipase domain, two lectin domains, and cholesterol-dependent cytolysin-related domain using various recombinant proteins. The gene was found not only in SPpn but also in the strains of SM and SPn. Moreover, the gene product was expressed in the gene-positive strains as secreted and cell-bound forms. The recombinant of gene product showed lipase activity and human cell-binding activity depending on the function of lectin domain(s), but no hemolytic activity. Thus, based on the distribution of the gene within the MGS and its molecular function, the gene product was named mitilectin (MLC) and its contribution to the potential pathogenicity of the MLC-producing strains was investigated. Consequently, the treatment with anti-MLC antibody and the mlc gene-knockout significantly reduced the human cell-binding activity of MLC-producing strains. Therefore, the multifunctional MLC was suggested to be important as an adhesion molecule in considering the potential pathogenicity of the MLC-producing strains belonging to MGS, such as SPpn and SM. (Link to Publication Site) ● Publication site (DOI): 10.1111/1348-0421.12868 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33331679 ● Search Scopus @ Elsevier (PMID): 33331679 ● Search Scopus @ Elsevier (DOI): 10.1111/1348-0421.12868 (DOI: 10.1111/1348-0421.12868, PubMed: 33331679)
10.	Atsushi Tabata, Hisashi Ohkuni, Yasuhiko Itoh, Yoshitaka Fukunaga, Toshifumi Tomoyasu and Hideaki Nagamune : Complete Genome Sequence of Streptococcus mitis Strain Nm-65, Isolated from a Patient with Kawasaki Disease, Microbiology Resource Announcements, Vol.10, No.1, e01239-20, 2021. (Summary) Nm-65 is a human commensal streptococcal strain of the mitis group that was isolated from the tooth surface of a patient with Kawasaki disease. The complete genome sequence of Nm-65 was obtained by means of hybrid assembly, using two next-generation sequencing data sets. The final assembly size was 2,085,837 bp, with 2,039 coding sequences. (Tokushima University Institutional Repository) ● Metadata: 116462 (Link to Publication Site) ● Publication site (DOI): 10.1128/MRA.01239-20 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33414340 ● Search Scopus @ Elsevier (PMID): 33414340 ● Search Scopus @ Elsevier (DOI): 10.1128/MRA.01239-20 (Tokushima University Institutional Repository: 116462, DOI: 10.1128/MRA.01239-20, PubMed: 33414340)
11.	Keita Hoshi, Yohei Sanagawa, Ryuta Umebayashi, Atsushi Tabata, Hideaki Nagamune, Eiji Hase, Takeo Minamikawa, Takeshi Yasui, Yasushi Yoshida, Keiji Minagawa, Yasuhiko Kawamura, Yasushi Imada and Fumitoshi Yagishita : Synthesis and Optical Property of Quadrupolar Pyridinium Salt and Its Application as Bioimaging Agent, Chemistry Letters, Vol.49, No.12, 1487-1489, 2020. (Link to Publication Site) ● Publication site (DOI): 10.1246/cl.200604 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1246/cl.200604 (DOI: 10.1246/cl.200604)
12.	Eiji Hase, Hiroki Takanari, Keita Hoshi, Masaki Okamoto, Atsushi Tabata, Hideaki Nagamune, Takeo Minamikawa, Takeshi Yasui, Yasushi Yoshida, Keiji Minagawa, Yasuhiko Kawamura, Yasushi Imada and Fumitoshi Yagishita : Two- and three-photon excitable quaternized imidazo[1,2-a]pyridines for mitochondrial imaging and potent cancer therapy agent, Organic & Biomolecular Chemistry, Vol.18, No.38, 7571-7576, 2020. (Link to Publication Site) ● Publication site (DOI): 10.1039/D0OB01585G (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32940324 ● Search Scopus @ Elsevier (PMID): 32940324 ● Search Scopus @ Elsevier (DOI): 10.1039/D0OB01585G (DOI: 10.1039/D0OB01585G, PubMed: 32940324)
13.	Atsushi Tabata, Hisashi Ohkuni, Haruka Hino, Takuya Saigo, Chihiro Kodama, QING TANG, Toshifumi Tomoyasu, Yoshitaka Fukunaga, Yasuhiko Itoh and Hideaki Nagamune : Cytotoxic property of Streptococcus mitis strain producing two different types of cholesterol-dependent cytolysins, Infection, Genetics and Evolution, Vol.85, 104483, 2020. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.meegid.2020.104483 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32731044 ● Summary page in Scopus @ Elsevier: 2-s2.0-85088967403 (DOI: 10.1016/j.meegid.2020.104483, PubMed: 32731044, Elsevier: Scopus)
14.	Ayuko Takao, Toshifumi Tomoyasu, Atsushi Tabata, Hideaki Nagamune and Nobuko Maeda : A photometric pH assay for microplate bacterial cultures., Journal of Microbiological Methods, Vol.172, 1-3, 2020. (Summary) A photometric pH assay for sugar-fermenting bacterial culture on a 96-well plate was developed. This assay can save time and effort in repeat handlings. Its use could decrease the risk of bacterial contamination in measurement devices and leakage into the environment. The assay's pH estimation range was pH 4.2-7.6. (Keyword) chemical indicators / fermentation / pH assay / photometric method / microbial culture (Link to Publication Site) ● Publication site (DOI): 10.1016/j.mimet.2020.105910 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32229266 ● Summary page in Scopus @ Elsevier: 2-s2.0-85082519032 (DOI: 10.1016/j.mimet.2020.105910, PubMed: 32229266, Elsevier: Scopus)
15.	Miho Kobayashi, Madoka Nishimura, Mina Kawamura, Norio Kamemura, Hideaki Nagamune and Atsushi Tabata : Change in membrane potential induced by streptolysin O, a pore-forming toxin: flow cytometric analysis using a voltage-sensitive fluorescent probe and rat thymic lymphocytes, Microbiology and Immunology, Vol.64, No.1, 10-22, 2020. (Summary) Streptolysin O (SLO) is a bacterial pore-forming toxin that is employed to permeabilize cell membranes in some biological experiments. SLO forms various types of pores with different shapes, increasing membrane ion permeability and subsequently inducing changes in membrane potential. To characterize the pores formed by SLO, the changes in membrane potential induced by SLO in rat lymphocytes were considered using flow cytometry with a voltage-sensitive fluorescent probe, bis-(1,3-dibutylbarbituric acid)trimethine oxonol (Oxonol). SLO caused three types of membrane potential responses accessed with Oxonol. One type induces a great decrease in Oxonol fluorescence (large hyperpolarization) that may be elicited via the increase of Ca -dependent K permeability by SLO-induced influx of external Ca . A second type is an increase in Oxonol fluorescence (depolarization) that may be caused by a nonspecific increase in membrane cation permeability. The third type is a small decrease in Oxonol fluorescence (small hyperpolarization), probably via an increase in Cl permeability. That SLO transitionally changes membrane ion permeability may have implications in the pathology of pyogenic group streptococci infections in which SLO is thought to be one of the key virulence factors. (Keyword) Animals / Bacterial Proteins / Bacterial Toxins / Barbiturates / Calcimycin / Calcium / Cell Membrane / Cell Membrane Permeability / Disease Models, Animal / Flow Cytometry / Fluorescent Dyes / Lymphocytes / Male / Membrane Potentials / Rats / Rats, Wistar / Streptolysins (Link to Publication Site) ● Publication site (DOI): 10.1111/1348-0421.12748 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31584206 ● Search Scopus @ Elsevier (PMID): 31584206 ● Search Scopus @ Elsevier (DOI): 10.1111/1348-0421.12748 (DOI: 10.1111/1348-0421.12748, PubMed: 31584206)
16.	Fumitoshi Yagishita, Jun-ichi Tanigawa, Chiho Nii, Atsushi Tabata, Hideaki Nagamune, Hiroki Takanari, Yasushi Imada and Yasuhiko Kawamura : Fluorescent Imidazo[1,5-a]pyridinium Salt for a Potential Cancer Therapy Agent, ACS Medicinal Chemistry Letters, Vol.10, No.8, 1110-1114, 2019. (Link to Publication Site) ● Publication site (DOI): 10.1021/acsmedchemlett.9b00034 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1021/acsmedchemlett.9b00034 (DOI: 10.1021/acsmedchemlett.9b00034)
17.	Atsushi Tabata, Takuya Yamada, Ohtani Hiromi, Kazuto Ohkura, Toshifumi Tomoyasu and Hideaki Nagamune : β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro, Journal of Oral Microbiology, Vol.11, No.1, 1-11, 2019. (Summary) Streptococcus anginosus subsp. (SAA) is one of the opportunistic pathogens in humans that inhabits the oral cavity. The type strain of SAA, NCTC10713, showed clear β-hemolysis on blood agar plates, and the sole β-hemolytic factor revealed two streptolysin S (SLS) molecules. SLS is well known as the peptide hemolysin produced from the human pathogen and shows not only hemolytic activity on erythrocytes but also cytotoxic activity in cell culture lines and , such as in a mouse infection model. However, no cytotoxic activity of SLS produced from β-hemolytic SAA (β-SAA) has been reported so far. In this study, the SLS-dependent cytotoxicity of the β-SAA strains including the genetically modified strains was investigated . The SLS-producing β-SAA showed cytotoxicity in human cell culture lines under the co-cultivation condition and it was found that this cytotoxicity was caused by the SLS secreted into the extracellular milieu. The results from this study suggest that the SLS produced from β-SAA might indicate the cytotoxic potential similar to that of the SLS from and the SLS-producing β-SAA would be recognized as "a wolf in sheep's clothing" More attention will be paid to the pathogenicity of β-hemolytic Anginosus group streptococci. (Keyword) bacterial toxins / Streptococcus (Tokushima University Institutional Repository) ● Metadata: 113540 (Link to Publication Site) ● Publication site (DOI): 10.1080/20002297.2019.1609839 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31105901 ● Summary page in Scopus @ Elsevier: 2-s2.0-85065585236 (Tokushima University Institutional Repository: 113540, DOI: 10.1080/20002297.2019.1609839, PubMed: 31105901, Elsevier: Scopus)
18.	Toshifumi Tomoyasu, Keigo Tsuruno, Ryosuke Tanatsugu, Aya Miyazaki, Hiroyuki Kondo, Atsushi Tabata, Whiley A. Robert, Kenji Sonomoto and Hideaki Nagamune : Recognizability of heterologous co-chaperones with Streptococcus intermedius DnaK and Escherichia coli DnaK, Microbiology and Immunology, Vol.62, No.11, 681-693, 2018. (Summary) Streptococcus intermedius DnaK complements the temperature-sensitive phenotype of an Escherichia coli dnaK null mutant only when co-chaperones DnaJ and GrpE are co-expressed. Therefore, whether S. intermedius DnaK and E. coli DnaK can recognize heterologous co-chaperones in vitro was examined. Addition of heterologous GrpE to DnaK and DnaJ partially stimulated adenosine triphosphatase (ATPase) activity, and almost completely stimulated luciferase refolding activity. Addition of heterologous DnaJ to GrpE and DnaK also stimulated ATPase activity; however, significant luciferase refolding activity was not observed. Moreover, E. coli DnaJ had a negative effect on the luciferase refolding activity of the S. intermedius DnaK chaperone system. In E. coli chaperone mutants, with the exception of E. coli DnaJ, stronger expression of the heterologous co-chaperones partially or almost completely complemented the temperature-sensitive-phenotype. These results indicate that all heterologous co-chaperones can at least partially recognize DnaK of a distantly related species. A region of the ATPase domain that is present in the DnaK of gram-negative bacteria is absent from the DnaK of gram-positive bacteria. This region is believed to be important for recognition of co-chaperones from gram-negative bacteria. However, insertion of this segment into S. intermedius DnaK failed to increase its ability to recognize E. coli co-chaperones, implying that this region is unnecessary or insufficient for the recognition of E. coli co-chaperones. Thus, our data suggest that a basic structural similarity is conserved among the components of the S. intermedius and E. coli DnaK chaperone systems, allowing weak associations between heterologous components. (Keyword) molecular chaperone / Escherichia coli / Streptococcus (Tokushima University Institutional Repository) ● Metadata: 115748 (Link to Publication Site) ● Publication site (DOI): 10.1111/1348-0421.12651 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30239035 ● Summary page in Scopus @ Elsevier: 2-s2.0-85056121796 (Tokushima University Institutional Repository: 115748, DOI: 10.1111/1348-0421.12651, PubMed: 30239035, Elsevier: Scopus)
19.	Fumitoshi Yagishita, Chiho Nii, Yoshihiko Tezuka, Atsushi Tabata, Hideaki Nagamune, Naohiro Uemura, Yasushi Yoshida, Takashi Mino, Masami Sakamoto and Yasuhiko Kawamura : Fluorescent N-Heteroarenes Having Large Stokes Shift and Water Solubility Suitable for Bioimaging, Asian Journal of Organic Chemistry, Vol.7, No.8, 1614-1619, 2018. (Link to Publication Site) ● Publication site (DOI): 10.1002/ajoc.201800250 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1002/ajoc.201800250 (DOI: 10.1002/ajoc.201800250)
20.	Qing Tang, Masayoshi Onitsuka, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Construction of Anti-HER2 Recombinants as Targeting Modules for a Drug-delivery System Against HER2-positive Cells, Anticancer Research, Vol.38, No.7, 4319-4325, 2018. (Summary) Recombinant antibodies have been investigated and used in applications such as targeting modules of drug-delivery systems (DDS) against cancers. This study aimed to prepare recombinant antibodies against HER2, containing sortase A (SrtA) recognition sequence, that are applicable as targeting modules in DDS after linkage with the drug-carrier containing oligoglycine-acceptor peptide by SrtA transpeptidation. The recombinant trastuzumab fragment antibodies (scFvs and Fab) with the SrtA-recognition motif (LPXTG) at their C-terminal were constructed and expressed in Escherichia coli and Chinese hamster ovary (CHO) cells, respectively. The reactivity of the purified recombinant antibodies towards HER2-expressing cells was also evaluated via immunofluorescent staining. Fab demonstrated higher yield and purity and better reactivity towards HER2-expressing cells (HCT-15 and HeLa) when compared to scFvs. The CHO expression system possesses superior yield and purity when compared to the E. coli expression system with respect to the preparation of recombinant antibodies applicable in targeting modules for DDS (DDS-TM). Moreover, a Fab variant prepared in this study demonstrated the potential to be a DDS-TM against HER2-expressing cancer cells. (Keyword) drug delivery system / cancer therapy (Link to Publication Site) ● Publication site (DOI): 10.21873/anticanres.12731 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29970568 ● Summary page in Scopus @ Elsevier: 2-s2.0-85049804969 (DOI: 10.21873/anticanres.12731, PubMed: 29970568, Elsevier: Scopus)
21.	Toshifumi Tomoyasu, Masaki Matoba, Ayuko Takao, Atsushi Tabata, A Robert. Whiley, Nobuko Maeda and Hideaki Nagamune : Rapid screening method for detecting highly pathogenic Streptococcus intermedius strains carrying a mutation in the lacR gene., FEMS Microbiology Letters, Vol.365, No.3, fnx-258, 2018. (Summary) Streptococcus intermedius is a member of the normal human commensal flora and secretes a human-specific cytolysin intermedilysin (ILY) as a major virulence factor. Expression of ily is repressed by LacR and loss-of-function mutations of LacR are observed in many ILY high-producing strains isolated from deep-seated abscesses, suggesting that high ILY production is necessary for increased virulence. However, because ILY exhibits no β-hemolysis on animal blood agar plates, differentiating ILY high- and low-producing strains using conventional laboratory methods is not possible. Interestingly, S. intermedius also produces glycosidases, including MsgA and NanA, which exhibit N-acetyl-β-d-glucosaminidase and neuraminidase activities, respectively. Moreover, MsgA expression, but not NanA, is negatively regulated by LacR. Here we measured the activities of MsgA, NanA and ILY in strains isolated from clinical specimens and dental plaque to determine the correlation between these glycosidase activities and ILY hemolytic activity. Hemolytic activity showed a strong positive correlation with MsgA and a weak negative correlation with NanA activities. Therefore, we calculated the ratio of MsgA and NanA activity (M/N ratio). This value showed a stronger positive correlation (r = 0.81) with ILY hemolytic activity and many strains with high M/N ratios (>2) were ILY-high producers with loss-of-function mutations in LacR. (Keyword) pathogenic microorganism / virulence factor (Tokushima University Institutional Repository) ● Metadata: 115749 (Link to Publication Site) ● Publication site (DOI): 10.1093/femsle/fnx258 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29228148 ● Summary page in Scopus @ Elsevier: 2-s2.0-85056596012 (Tokushima University Institutional Repository: 115749, DOI: 10.1093/femsle/fnx258, PubMed: 29228148, Elsevier: Scopus)
22.	Atsushi Tabata, Douglas Deutsch, Seiya Otsuka, Kathleen Verratti, Toshifumi Tomoyasu, Hideaki Nagamune and A. Vincent Fischetti : A novel plasmid, pSAA0430-08, from Streptococcus anginosus subsp. anginosus strain 0430-08, Plasmid, Vol.95, No.1, 16-27, 2018. (Summary) Mobile genetic elements (MGEs) are the genetic material often involved in the interspecies and intraspecies genetic transduction in bacteria. However, little is known about MGEs in the Anginosus group of streptococci (AGS), one of the streptococcal groups found in the oral cavity of humans. We looked for the presence of MGEs in Streptococcus anginosus subsp. anginosus (SAA), a representative species belonging to AGS, and found a novel plasmid from SAA strain 0430-08. This plasmid was 7038bp and ~31% G/C content which we named pSAA0430-08, and examined its genetic structure and characteristics. Open reading frame (ORF) prediction revealed that pSAA0430-08 was composed of 10 ORFs including a putative plasmid replication protein (ORF1) and a putative toxin-antitoxin system (ORF9 and ORF10). Between ORF10 and ORF 1, four tandem repeats of 22bp each, generally termed as iteron, were also observed. Using variant plasmids of pSAA0430-08, we confirmed that both ORF1 and iteron were necessary for replication in host cells. Interestingly, the region from ORF4 to ORF7 showed homology with a genomic DNA segment of S. gordonii strains. Thus, this plasmid may travel between the different species in Streptococci, i.e., S. gordonii and S. anginosus. (Keyword) pathogenic microorganism / Plasmid (Link to Publication Site) ● Publication site (DOI): 10.1016/j.plasmid.2018.01.002 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29355570 ● Summary page in Scopus @ Elsevier: 2-s2.0-85041495681 (DOI: 10.1016/j.plasmid.2018.01.002, PubMed: 29355570, Elsevier: Scopus)
23.	Tatematsu Yohei, Fujita Haruhi, Hayashi Hiroki, Yamamoto Atsushi, Atsushi Tabata, Hideaki Nagamune and Ohkura Kazuto : Effects of the Nonsteroidal Anti-inflammatory Drug Celecoxib on Mitochondrial Function, Biological & Pharmaceutical Bulletin, Vol.41, No.3, 319-325, 2018. (Summary) Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used to treat inflammation and pain. In the present study, we examined the effects of celecoxib, a cyclooxygenase-2 (COX-2)-selective NSAID, on rat liver mitochondrial function. Celecoxib dose-dependently induced mitochondria swelling, which was not suppressed by cyclosporine A (CsA). The oxygen consumption rate in mitochondria-suspended solution was facilitated by the addition of celecoxib, and its uncoupling activity was observed. Celecoxib also suppressed SF6847-induced uncoupling, and appeared to exert inhibitory effects on the electron transport chain. Celecoxib suppressed the state 3 oxygen consumption rate in the presence of ADP. Protein release from the mitochondrial matrix was detected following the addition of celecoxib, and aldehyde dehydrogenase 2 (ALDH2) and hydroxymethylglutaryl-CoA (HMG-CoA) synthase 2 (HMGCS2) bands were confirmed in a Western blot analysis. On the other hand, protein release of cytochrome C (CytC), which is an inducer of apoptosis, from the intermembrane space was not observed. Celecoxib enhanced the membrane permeability of human erythrocytes and synthesized liposomes dose-dependently. It then induced the membrane-involving mitochondrial swelling and suppressed mitochondrial function. (Keyword) Aldehyde Dehydrogenase, Mitochondrial / Animals / Anti-Inflammatory Agents, Non-Steroidal / Celecoxib / Cell Membrane Permeability / Cyclooxygenase 2 Inhibitors / Dose-Response Relationship, Drug / Electron Transport / Erythrocytes / Hydroxymethylglutaryl-CoA Synthase / In Vitro Techniques / Male / Mitochondria / Mitochondria, Liver / Mitochondrial Swelling / Oxygen Consumption / Rats / Rats, Wistar (Link to Publication Site) ● Publication site (DOI): 10.1248/bpb.b17-00527 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29491208 ● Summary page in Scopus @ Elsevier: 2-s2.0-85050992357 (DOI: 10.1248/bpb.b17-00527, PubMed: 29491208, Elsevier: Scopus)
24.	Toshifumi Tomoyasu, Takahiro Yamasaki, Shinya Chiba, Shingo Kusaka, Atsushi Tabata, Robert A. Whiley and Hideaki Nagamune : Positive- and Negative-Control Pathways by Blood Components for Intermedilysin Production in Streptococcus intermedius., Infection and Immunity, Vol.85, No.9, 1-17, 2017. (Summary) is an opportunistic bacterial pathogen secreting a human-specific cytolysin called intermedilysin (ILY) as a major pathogenic factor. This bacterium can degrade glycans into monosaccharides using two glycosidases, multisubstrate glycosidase A (MsgA) and neuraminidase (NanA). Here, we detected a stronger hemolytic activity mediated by ILY when PC574 was cultured in fetal bovine serum (FBS) than when it was grown in the standard culture medium. FBS-cultured cells also showed higher MsgA and NanA activity, although overproduction of ILY in FBS was undetectable in mutants -null and -null. Addition of purified MsgA and NanA to the FBS resulted in a release of 2.8 mM galactose and 4.3 mM -acetylneuraminic acid; these sugar concentrations were sufficient to upregulate the expression of ILY, MsgA, and NanA. Conversely, when strain PC574 was cultured in human plasma, no similar increase in hemolytic activity was observed. Moreover, addition of human plasma to the culture in FBS appeared to inhibit the stimulatory effect of FBS on ILY, MsgA, and NanA, although there were individual differences among the plasma samples. We confirmed that human plasma contains immunoglobulins that can neutralize ILY, MsgA, and NanA activities. In addition, human plasma had a neutralizing effect on cytotoxicity of toward HepG2 cells in FBS, and a higher concentration of human plasma was necessary to reduce the cytotoxicity of an ILY-high-producing strain than an ILY-low-producing strain. Overall, our data show that blood contains factors that stimulate and inhibit ILY expression and activity, which may affect pathogenicity of . (Keyword) Streptococcus intermedius / intermedilysin / cytolysin / sugar chain / antibody (Link to Publication Site) ● Publication site (DOI): 10.1128/IAI.00379-17 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28607101 ● Summary page in Scopus @ Elsevier: 2-s2.0-85027524694 (DOI: 10.1128/IAI.00379-17, PubMed: 28607101, Elsevier: Scopus)
25.	Keiji Murakami, Hiromichi Yumoto, Ayu Murakami, Takashi Amoh, Darija Viducic, Katsuhiko Hirota, Atsushi Tabata, Hideaki Nagamune, Hiroki Kourai, Takashi Matsuo and Yoichiro Miyake : Evaluation of the effectiveness of the potent bis-quaternary ammonium compound, 4,4'-( , -hexametylenedithio) bis (1-octylpyridinium bromide) (4DTBP-6,8) on Pseudomonas aeruginosa., Journal of Applied Microbiology, Vol.122, No.4, 893-899, 2017. (Summary) Quaternary ammonium compounds (QACs), including benzalkonium chloride (BAC) and cetylpyridinium chloride (CPC) are cationic surfactants and have been used widely as general disinfectants in the medical field due to their strong antibacterial effects and low cytotoxicity to human cells. 4,4'-( , -hexametylenedithio) bis (1-octylpyridinium bromide) (4DTBP-6,8) is one of the potent bis-QACs synthesized to improve the antimicrobial activities of mono-QACs such as BAC. This study aimed to assess the effectiveness of 4DTBP-6,8 against Pseudomonas aeruginosa, a prevalent hospital pathogen. The minimum inhibitory concentrations of 4DTBP-6,8, CPC and BAC against P. aeruginosa were measured. 4DTBP-6,8 exhibited strong antibacterial activity. We assessed the bactericidal effects of QACs against P. aeruginosa under certain conditions and their cytotoxicities in human epithelial cells using lactate dehydrogenase (LDH) release. 4DTBP-6,8 exerted excellent bactericidal effects against high concentrations of bacteria, biofilm cells and even in the presence of contaminated proteins. Cellular LDH was not released by the treatment with 4DTBP-6,8. 4DTBP-6,8 exhibited the strongest bactericidal activity against P. aeruginosa among the three QACs tested without any cytotoxicity. The potent bis-QAC, 4DTBP-6,8 has the potential to be an effective disinfectant in preventing hospital infections caused by P. aeruginosa. (Keyword) Anti-Bacterial Agents / Biofilms / Cell Line / Disinfectants / Humans / Niacinamide / Pseudomonas aeruginosa / Pyridinium Compounds (Link to Publication Site) ● Publication site (DOI): 10.1111/jam.13392 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28035713 ● Summary page in Scopus @ Elsevier: 2-s2.0-85013236835 (DOI: 10.1111/jam.13392, PubMed: 28035713, Elsevier: Scopus)
26.	Yamamoto Masashi, Takami Takashi, Matsumura Reiko, Dorofeev Alexander, Hirata Yoshihiko and Hideaki Nagamune : In Vitro Evaluation of the Biocompatibility of Newly Synthesized Bis-Quaternary Ammonium Compounds with Spacer Structures Derived from Pentaerythritol or Hydroquinone, Biocontrol Science, Vol.21, No.4, 231-241, 2016. (Summary) With the object of developing new biocides milder for human use than the current antiseptics, we synthesized a series of bis-quaternary ammonium compounds (bis-QACs). The antimicrobial activity of the newly synthesized bis-QACs and common biocides used as antiseptics was compared by examining minimum inhibitory concentrations and minimum bactericidal concentrations (MBCs). Moreover, the cytotoxicity of these compounds against human cells was determined to calculate the biocompatibility index (BI) of these compounds. BI was the ratio of the concentration of a biocide giving a 50% lethal effect on normal human epidermal keratinocytes to its MBC against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The commonly used antiseptics tested were benzalkonium chloride (BAC), octenidine dihydrochloride (OCT), chlorhexidine digluconate (CHG) and polyhexamethylene biguanide (PHMB). In comparison with these antiseptics, it was shown that some of new bis-QACs exhibited a wider and more potent antimicrobial spectrum than OCT. The cytotoxicity of these bis-QACs was equal or lower compared to that of the quaternary ammonium compounds (BAC and OCT), although these bis-QACs showed higher toxicity than the biguanide-based compounds (CHG and PHMB). Finally, the comparison of BIs revealed that new bis-QACs such as N-dodecyl {4,4'-(2,4,8,10-tetraoxaspiro[5.5]undecan-3,9-diyl) }dipyridinium dibromide (4TOSU-12), 3,3'-[1,4-Phenylenebis (oxy)]bis (1-dodecylpyridinium) dibromide (3PHBO-12) and 3-(3-Hydroxy-2-(hydroxymethyl)-2-{[(1-dodecylpyridinium-3-yl) oxy]methyl}propoxy)-1-dodecylpyridinium dibromide (3HHDMP-12) had equal or greater biocompatibility than the commonly used biocides tested. Thus, these results strongly suggested that 4TOSU-12, 3PHBO-12 and 3HHDMP-12 could be useful as antiseptics for topical application to the skin. (Keyword) Bis-quaternary ammonium compounds / Antimicrobial activity / Cytotoxicity assay / Antiseptic (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.21.231 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28003630 ● Summary page in Scopus @ Elsevier: 2-s2.0-85007028365 (DOI: 10.4265/bio.21.231, PubMed: 28003630, Elsevier: Scopus)
27.	Yuki Kawaguchi, Yohei Tatematsu, Atsushi Tabata, Hideaki Nagamune and Kazuto Ohkura : Cytolytic Activity and Molecular Feature of Cardiotoxin and Cardiotoxin-like Basic Protein: The Electrostatic Potential Field Is an Important Factor for Cell Lytic Activity, Anticancer Research, Vol.35, No.8, 4515-4519, 2015. (Summary) Cardiotoxin (CT) is a well-known cell lytic protein and has been purified from cobra venom. Cardiotoxin-like basic protein (CLBP) has two amino acid insertions and does not exhibit cell lytic activity. The molecular features of these CT family proteins were examined in the present study using molecular modeling and molecular simulation techniques. Molecular models of CT and CLBP were constructed based on the X-ray data of Naja mossambica mossambica CT VII4 (Protein Data Bank ID: 1CDT). The structural features of these models were examined using molecular orbital and electrostatic potential parameters. The stereo-hydrophobicities and molecular torsions of CT and CLBP, which are indexes of structural features, were similar. Electrostatic potential fields (ESP) differed between CT and CLBP and this was considered one of the critical factors in molecular titer. The distribution of ESP fields may affect the cytolytic activity of the CT family. (Keyword) Amino Acid Sequence / Animals / Cobra Cardiotoxin Proteins / Elapidae / Hydrophobic and Hydrophilic Interactions / Models, Molecular / Molecular Sequence Data / Protein Conformation / Sequence Alignment / Static Electricity (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26168495 ● Summary page in Scopus @ Elsevier: 2-s2.0-84939786315 (PubMed: 26168495, Elsevier: Scopus)
28.	Atsushi Tabata, Ohkubo Yukihisa, Natsuki Anyhoji, Keiko Hojo, Toshifumi Tomoyasu, Youhei Tatematsu, Kazuto Ohkura and Hideaki Nagamune : Development of a Sortase A-mediated Peptide-labeled Liposome Applicable to Drug-delivery Systems, Anticancer Research, Vol.35, No.8, 4411-4417, 2015. (Summary) In order to develop an efficient drug-delivery system (DDS), a lipopeptide-loaded liposome that functions as a platform for the transpeptidase reaction mediated by sortase A (SrtA) was constructed and its stability, as well as cell-specific targeting were evaluated in the present study. Several lipopeptides possessing an acceptor peptide sequence (oligoglycine ≥ three residues) or donor peptide sequence (LPETG) for the SrtA-mediated reaction were chemically synthesized and then inserted into the liposome membrane composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and cholesterol (DPPC-Chol-lipo) to obtain the lipopeptide-loaded liposomes. The transpeptidase reaction mediated by recombinant SrtA (His-ΔN59SrtA) was employed to modify the peptide moiety on the liposomal surface using a fluorescently-labeled substrate peptide corresponding to the species of each loaded lipopeptide. Furthermore, lung tumor-binding peptide (LTBP)-labeled liposomes, prepared by this transpeptidase reaction, were investigated for selective targeting to lung cancer cells in vitro. The His-ΔN59SrtA-mediated transpeptidation of fluorescently-labeled peptide on the lipopeptide-loaded DPPC-Chol-lipo was confirmed. The selective targeting of LTBP-labeled liposomes to the lung cancer cell line A549 was also observed in vitro. These results suggest that the labeling of acceptor or donor lipopeptide-loaded liposomes with the transpeptidase SrtA could be a useful method for developing a platform applicable to a cancer-targeting DDS. (Keyword) drug delivery system / liposomes (Tokushima University Institutional Repository) ● Metadata: 117781 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26168480 ● Summary page in Scopus @ Elsevier: 2-s2.0-84939824857 (Tokushima University Institutional Repository: 117781, PubMed: 26168480, Elsevier: Scopus)
29.	Onodera Takefumi, Kuriyama Isoko, Sakamoto Yuka, Kawamura Moe, Kuramochi Kouji, Tsubaki Kazunori, Atsushi Tabata, Hideaki Nagamune and Mizushina Yoshiyuki : 5-O-Acyl plumbagins inhibit DNA polymerase activity and suppress the inflammatory response, Archives of Biochemistry and Biophysics, Vol.573, 100-110, 2015. (Summary) We previously found that vitamin K3 (menadione, 2-methyl-1,4-naphthoquinone) inhibits the activity of human mitochondrial DNA polymerase (pol) γ. In this study, we focused on plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone), and chemically synthesized novel plumbagins conjugated with C2:0 to C22:6 fatty acids (5-O-acyl plumbagins). These chemically modified plumbagins displayed enhanced mammalian pol inhibition, with plumbagin conjugated to docosahexaenoic acid (C22:6-acyl plumbagin) exhibiting the strongest inhibition of pol λ among the ten 5-O-acyl plumbagins synthesized. C22:6-acyl plumbagin selectively inhibited the activities of mammalian pol species, but did not influence the activities of other pols or DNA metabolic enzymes tested. The inhibition of pol λ, a DNA repair/recombination pol, by these compounds was significantly correlated with both their suppression of lipopolysaccharide (LPS) induced tumor necrosis factor-α (TNF-α) production by mouse RAW264.7 macrophages and the reduction of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in the mouse ear. These data indicate that 5-O-acyl plumbagins act as anti-inflammatory agents by inhibiting mammalian pol λ. These results further suggest that C22:6-acyl plumbagin is a promising anti-inflammatory candidate and that acylation could be an effective chemical modification to improve the anti-inflammatory activity of vitamin K3 derivatives, such as plumbagin. (Keyword) Animals / Anti-Inflammatory Agents / Cell Line / DNA Polymerase beta / Escherichia coli Proteins / Fatty Acids / Gene Knockdown Techniques / Humans / Inflammation / Lipopolysaccharides / Macrophages / Mice / Naphthoquinones / Plant Proteins / Rats / Tetradecanoylphorbol Acetate / Tumor Necrosis Factor-alpha (Link to Publication Site) ● Publication site (DOI): 10.1016/j.abb.2015.02.032 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25748000 ● Summary page in Scopus @ Elsevier: 2-s2.0-84926219634 (DOI: 10.1016/j.abb.2015.02.032, PubMed: 25748000, Elsevier: Scopus)
30.	Kim Hyejin, Atsushi Tabata, Toshifumi Tomoyasu, Ueno Tomomi, Uchiyama Shigeto, Keizo Yuasa, Akihiko Tsuji and Hideaki Nagamune : Estrogen stimuli promote osteoblastic differentiation via the subtilisin-like proprotein convertase PACE4 in MC3T3-E1 cells., Journal of Bone and Mineral Metabolism, Vol.33, No.1, 30-39, 2015. (Summary) Estrogenic compounds include endogenous estrogens such as estradiol as well as soybean isoflavones, such as daidzein and its metabolite equol, which are known phytoestrogens that prevent osteoporosis in postmenopausal women. Indeed, mineralization of MC3T3-E1 cells, a murine osteoblastic cell line, was significantly decreased in medium containing fetal bovine serum treated with charcoal-dextran to deplete endogenous estrogens, but estradiol and these soybean isoflavones dose-dependently restored the differentiation of MC3T3-E1 cells; equal was tenfold more effective than daidzein. These differentiation promoting effects were inhibited by the addition of fulvestrant, which is a selective downregulator of estrogen receptors. Analysis of the expression pattern of bone-related genes by reverse transcription PCR (RT-PCR)/quantitative real-time PCR (qRT-PCR), which focused on responsiveness to the estrogen stimuli, revealed that the transcription of PACE4, a subtilisin-like proprotein convertase, was tightly linked with the differentiation of MC3T3-E1 cells induced by estrogen stimuli. Moreover, treatment with RNAi of PACE4 in MC3T3-E1 cells resulted in a drastic decrease of mineralization in the presence of estrogen stimuli. These results strongly suggest that PACE4 participates in bone formation at least in osteoblast differentiation, and estrogen receptor-mediated stimuli induce osteoblast differentiation through the upregulation of PACE4 expression. (Keyword) osteoblast / PACE4 / Estrogen / Soybean / isoflavone (Link to Publication Site) ● Publication site (DOI): 10.1007/s00774-014-0567-9 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24557631 ● Summary page in Scopus @ Elsevier: 2-s2.0-84893948353 (DOI: 10.1007/s00774-014-0567-9, PubMed: 24557631, Elsevier: Scopus)
31.	Kawaguchi Yuki, Atsushi Tabata, Hideaki Nagamune and Kazuto Ohkura : Molecular Analysis of Streptococcus anginosus-derived SagA Peptides, Anticancer Research, Vol.34, No.8, 4627-4631, 2014. (Summary) SagA1 and SagA2 molecules produced from beta-hemolytic Streptococcus anginosus subsp. anginosus are composed of a leader peptide and a propeptide, and their mature form has hemolytic activity as a well-known Streptococcal peptide toxin, streptolysin. The function of these SagA molecules is thought to be dependent on intra-molecular heterocycle formation. In this study, we examined the heterocycle-involved molecular features of SagA1, SagA2, and S. pyogenes SagA (SPySagA), focusing on their heterocycle formation. Molecular models of SagA1, SagA2, and SPySagA were constructed using a molecular modeling technique. Molecular dynamics and molecular mechanic analyses of the modeled SagA molecules were performed to obtain their energy profiles. Total energy of the modeled SagA1, SagA2, and SPySagA decreased with heterocycle formation, and the border between the leader peptide and propeptide was clearly observed after heterocycle formation. The flexibility of SagA molecules was changed by intramolecular heterocycle formation, and their function (e.g. hemolytic activity) seems to be regulated by structural transition with heterocycle formation. (Keyword) SagA / Streptolysin S / Streptococcus anginosus (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25075110 ● Summary page in Scopus @ Elsevier: 2-s2.0-84908662068 (PubMed: 25075110, Elsevier: Scopus)
32.	Atsushi Tabata, Natsuki Anyoji, Yukimasa Ohkubo, Toshifumi Tomoyasu and Hideaki Nagamune : Investigation on the Reaction Conditions of Staphylococcus aureus Sortase A for Creating Surface-modified Liposomes as a Drug-delivery System Tool, Anticancer Research, Vol.34, No.8, 4521-4527, 2014. (Summary) This study aimed to determine the preferred conditions for the transpeptidase reaction of sortase A from Staphylococcus aureus, for the purpose of creating functional liposomes useful for a drug-delivery system (DDS). His-tagged recombinant sortase A with 59 amino acids deleted from the N-terminus (His-ΔN59SrtA) was prepared using an Escherichia coli expression system. The pH dependency and sorting signal sequence dependency of the transpeptidase reaction of His-ΔN59SrtA were analyzed by monitoring the transfer of model donor-substrates (i.e. His-tagged mutant green fluorescent proteins with a C-terminal LPxTG sorting signal) to model acceptor-beads with a GGGGGC peptide. In addition, using preferred conditions, the sortase A reaction was used to modify liposome surface. The transpeptidase reaction of His-ΔN59SrtA was enhanced under weakly acidic conditions. Transfer efficiency, based on sorting signal recognition by His-ΔN59SrtA, was similar to or higher than that obtained using several substrates with amino acids other than Glu in the sorting signal position "x". Furthermore, liposomes containing GGGGGC peptide-linked dipalmitoylphosphatidylethanolamine were successfully modified using the preferred conditions for His-ΔN59SrtA determined in this study. Preferred conditions for the transpeptidase reaction of His-ΔN59SrtA, especially in a weakly acidic environment to enhance reaction, was established and successfully used to create functional liposomes applicable to DDS. (Keyword) Sortase A (Tokushima University Institutional Repository) ● Metadata: 117782 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25075095 ● Summary page in Scopus @ Elsevier: 2-s2.0-84908660909 (Tokushima University Institutional Repository: 117782, PubMed: 25075095, Elsevier: Scopus)
33.	Hidenori Imaki, Toshifumi Tomoyasu, Naoki Yamamoto, Chiharu Taue, Sachiko Masuda, Ayuko Takao, Nobuko Maeda, Atsushi Tabata, Robert A. Whiley and Hideaki Nagamune : Identification and Characterization of a Novel Secreted Glycosidase with Multiple Glycosidase Activities in Streptococcus intermedius, Journal of Bacteriology, Vol.196, No.15, 2817-2826, 2014. (Summary) Streptococcus intermedius is a known human pathogen and belongs to the anginosus group (S. anginosus, S. intermedius, and S. constellatus) of streptococci (AGS). We found a large open reading frame (6,708 bp) in the lac operon, and bioinformatic analysis suggested that this gene encodes a novel glycosidase that can exhibit β-d-galactosidase and N-acetyl-β-d-hexosaminidase activities. We, therefore, named this protein "multisubstrate glycosidase A" (MsgA). To test whether MsgA has these glycosidase activities, the msgA gene was disrupted in S. intermedius. The msgA-deficient mutant no longer showed cell- and supernatant-associated β-d-galactosidase, β-d-fucosidase, N-acetyl-β-d-glucosaminidase, and N-acetyl-β-d-galactosaminidase activities, and all phenotypes were complemented in trans with a recombinant plasmid carrying msgA. Purified MsgA had all four of these glycosidase activities and exhibited the lowest Km with 4-methylumbelliferyl-linked N-acetyl-β-d-glucosaminide and the highest kcat with 4-methylumbelliferyl-linked β-d-galactopyranoside. In addition, the purified LacZ domain of MsgA had β-d-galactosidase and β-d-fucosidase activities, and the GH20 domain exhibited both N-acetyl-β-d-glucosaminidase and N-acetyl-β-d-galactosaminidase activities. The β-d-galactosidase and β-d-fucosidase activities of MsgA are thermolabile, and the optimal temperature of the reaction was 40°C, whereas almost all enzymatic activities disappeared at 49°C. The optimal temperatures for the N-acetyl-β-d-glucosaminidase and N-acetyl-β-d-galactosaminidase activities were 58 and 55°C, respectively. The requirement of sialidase treatment to remove sialic acid residues of the glycan branch end for glycan degradation by MsgA on human α1-antitrypsin indicates that MsgA has exoglycosidase activities. MsgA and sialidase might have an important function in the production and utilization of monosaccharides from oligosaccharides, such as glycans for survival in a normal habitat and for pathogenicity of S. intermedius. (Keyword) Streptococcus intermedius / glycosidase / multi-substrates glycosidase (Link to Publication Site) ● Publication site (DOI): 10.1128/JB.01727-14 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24858187 ● Summary page in Scopus @ Elsevier: 2-s2.0-84903904703 (DOI: 10.1128/JB.01727-14, PubMed: 24858187, Elsevier: Scopus)
34.	Atsushi Tabata, Yuji Sato, Kentaro Maya, Kota Nakano, Ken Kikuchi, Robert A. Whiley, Kazuto Ohkura, Toshifumi Tomoyasu and Hideaki Nagamune : A streptolysin S homologue is essential for beta-haemolytic Streptococcus constellatus subsp. constellatus cytotoxicit, Microbiology, Vol.160, No.5, 980-991, 2014. (Summary) Streptococcus constellatus is a member of the Anginosus group streptococci (AGS) and primarily inhabits the human oral cavity. S. constellatus is composed of three subspecies: S. constellatus subsp. constellatus (SCC), S. constellatus subsp. pharyngis and the newly described subspecies S. constellatus subsp. viborgensis. Although previous studies have established that SCC contains β-haemolytic strains, the factor(s) responsible for β-haemolysis in β-haemolytic SCC (β-SCC) has yet to be clarified. Recently, we discovered that a streptolysin S (SLS) homologue is the β-haemolytic factor of β-haemolytic Streptococcus anginosus subsp. anginosus (β-SAA), another member of the AGS. Furthermore, because previous studies have suggested that other AGS species, except for Streptococcus intermedius, do not possess a haemolysin(s) belonging to the family of cholesterol-dependent cytolysins, we hypothesized that, as with β-SAA, the SLS homologue is the β-haemolytic factor of β-SCC, and therefore aimed to investigate and characterize the haemolytic factor of β-SCC in the present study. PCR amplification revealed that all of the tested β-SCC strains were positive for the sagA homologue of SCC (sagA(SCC)). Further investigations using β-SCC strain W277 were conducted to elucidate the relationship between sagA(SCC) and β-haemolysis by constructing sagA(SCC) deletion mutants, which completely lost β-haemolytic activity. This loss of β-haemolytic activity was restored by trans-complementation of sagA(SCC). Furthermore, a co-cultivation assay established that the cytotoxicity of β-SCC was clearly dependent on the presence of sagA(SCC). These results demonstrate that sagA(SCC) is the factor responsible for β-SCC β-haemolysis and cytotoxicity. (Keyword) Streptolysin S / Streptococcus constellatus / beta-hemolytic / cytotoxicity (Link to Publication Site) ● Publication site (DOI): 10.1099/mic.0.075580-0 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24600025 ● Search Scopus @ Elsevier (PMID): 24600025 ● Search Scopus @ Elsevier (DOI): 10.1099/mic.0.075580-0 (DOI: 10.1099/mic.0.075580-0, PubMed: 24600025)
35.	Atsushi Tabata, Kazuto Ohkura, Ohkubo Yukimasa, Toshifumi Tomoyasu, Ohkuni Hisashi, Whiley A. Robert and Hideaki Nagamune : The diversity of receptor recognition in cholesterol-dependent cytolysins, Microbiology and Immunology, Vol.58, No.3, 155-171, 2014. (Summary) Cholesterol-dependent cytolysins (CDCs) are bacterial pore-forming toxins secreted mainly by pathogenic Gram-positive bacteria. CDCs generally recognize and bind to membrane cholesterol to create pores and lyse target cells. However, in contrast to typical CDCs such as streptolysin O, several atypical CDCs have been reported. The first of these was intermedilysin, which is secreted by Streptococcus intermedius and has human cell-specificity, human CD59 (huCD59) being its receptor. In the study reported here, the diversity of receptor recognition among CDCs was investigated and multi-receptor recognition characteristics were identified within this toxin family. Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) secreted by S. mitis strain Nm-65 isolated from a patient with Kawasaki disease was previously shown to hemolyze erythrocytes in a species-dependent manner, its maximum activity being in human cells. In the present study, it was found that Sm-hPAF recognizes both membrane cholesterol and huCD59 as receptors for triggering pore-formation. Moreover, vaginolysin (VLY) of Gardnerella vaginalis showed similar characteristics to Sm-hPAF regarding receptor recognition. On the basis of the results presented here, the mode of receptor recognition of CDCs can be categorized into the following three groups: (i) Group I, comprising typical CDCs with high affinity to cholesterol and no or very little affinity to huCD59; (ii) Group II, including atypical CDCs such as ILY, with no or very little affinity to cholesterol and high affinity to huCD59; and (iii) Group III, which contains atypical CDCs such as Sm-hPAF and VLY with affinity to both cholesterol and huCD59. (Keyword) cytolysin / cholesterol-dependet cytolysin / diversity (Link to Publication Site) ● Publication site (DOI): 10.1111/1348-0421.12131 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24401114 ● CiNii @ National Institute of Informatics (CRID): 1520291853530158464 ● Summary page in Scopus @ Elsevier: 2-s2.0-84895562361 (DOI: 10.1111/1348-0421.12131, PubMed: 24401114, CiNii: 1520291853530158464, Elsevier: Scopus)
36.	Toshifumi Tomoyasu, Hidenori Imaki, Sachiko Masuda, Ayumi Okamoto, HyeJin Kim, Richard Waite, Robert Whiley, Ken Kikuchi, Keiichi Hiramatsu, Atsushi Tabata and Hideaki Nagamune : LacR mutations are frequently observed in Streptococcus intermedius and are responsible for increased intermedilysin production and virulence., Infection and Immunity, Vol.81, No.9, 3276-3286, 2013. (Summary) Streptococcus intermedius secretes a human-specific cytolysin, intermedilysin (ILY), which is considered to be the major virulence factor of this pathogen. We screened for a repressor of ily expression by using random gene disruption in a low-ILY-producing strain (PC574). Three independent high-ILY-producing colonies that had plasmid insertions within a gene that has high homology to lacR were isolated. Validation of these observations was achieved through disruption of lacR in strain PC574 with an erythromycin cassette, which also led to higher hemolytic activity, increased transcription of ily, and higher cytotoxicity against HepG2 cells, compared to the parental strain. The direct binding of LacR within the ily promoter region was shown by a biotinylated DNA probe pulldown assay, and the amount of ILY secreted into the culture supernatant by PC574 cells was increased by adding lactose or galactose to the medium as a carbon source. Furthermore, we examined lacR nucleotide sequences and the hemolytic activity of 50 strains isolated from clinical infections and 7 strains isolated from dental plaque. Of the 50 strains isolated from infections, 13 showed high ILY production, 11 of these 13 strains had one or more point mutations and/or an insertion mutation in LacR, and almost all mutations were associated with a marked decline in LacR function. These results strongly suggest that mutation in lacR is required for the overproduction of ILY, which is associated with an increase in pathogenicity of S. intermedius. (Keyword) Streptococcus / pathogenic microorganism / virulence factor / gene expression (Link to Publication Site) ● Publication site (DOI): 10.1128/IAI.00638-13 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23798532 ● Summary page in Scopus @ Elsevier: 2-s2.0-84884217618 (DOI: 10.1128/IAI.00638-13, PubMed: 23798532, Elsevier: Scopus)
37.	Kawaguchi Yuki, Atsushi Tabata, Hideaki Nagamune and Kazuto Ohkura : Profiles of ILY, VLY and Sm-hPAF interaction with human CD59, Anticancer Research, Vol.33, No.7, 2901-2904, 2013. (Summary) The molecular features of a new member of the bacterially -derived cytolysin family were examined. In particular, the interactive mechanisms of intermedilysin (ILY), vaginolysin (VLY), and Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) with human CD59 (hCD59) were analyzed. Molecular models of VLY and Sm-hPAF were constructed based on X-ray data of ILY (protein data bank ID=1S3R), and their interactive profiles with hCD59 were examined using molecular simulation. Non-binding (NB) energy between ILY and hCD59 was three orders of magnitude higher than the energy between VLY and hCD59. NB energy between Sm-hPAF and hCD59 was similar to that between VLY and hCD59. A hydrogen bond (ILY Arg432-hCD59 Glu76) was observed between ILY and hCD59, and a stronger interaction was formed by flexible adjustment between them. (Keyword) cytolysin / cholesterol / human-specific / intermedilysin / CD59 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23780977 ● Search Scopus @ Elsevier (PMID): 23780977 (PubMed: 23780977)
38.	Atsushi Tabata, Yukimasa Ohkubo, Masato Tamura, Toshifumi Tomoyasu, Kazuto Ohkura and Hideaki Nagamune : Construction of an improved drug delivery system tool with enhanced versatility in cell-targeting., Anticancer Research, Vol.33, No.7, 2905-2910, 2013. (Summary) His-Z-CDC(ss)(IS) was revealed to be an improved DDS tool with enhanced versatility in cell targeting. (Keyword) Carcinoembryonic Antigen / Cell Membrane / Cells, Cultured / cholesterol / Drug Delivery Systems / Fibroblasts / Fluorouracil / Hemolysis / Hep G2 Cells / Humans / Immunoglobulin G / liposomes / Perforin / Protein Transport / Recombinant Fusion Proteins / Staphylococcus aureus (Tokushima University Institutional Repository) ● Metadata: 117783 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23780978 ● Search Scopus @ Elsevier (PMID): 23780978 (Tokushima University Institutional Repository: 117783, PubMed: 23780978)
39.	Atsushi Tabata, Kota Nakano, Kazuto Ohkura, Toshifumi Tomoyasu, Ken Kikuchi, Robert Whiley and Hideaki Nagamune : Novel Twin Streptolysin S-Like Peptides Encoded in the sag Operon Homologue of Beta-Hemolytic Streptococcus anginosus., Journal of Bacteriology, Vol.195, No.5, 1090-1099, 2013. (Summary) Streptococcus anginosus is a member of the anginosus group streptococci, which form part of the normal human oral flora. In contrast to the pyogenic group streptococci, our knowledge of the virulence factors of the anginosus group streptococci, including S. anginosus, is not sufficient to allow a clear understanding of the basis of their pathogenicity. Generally, hemolysins are thought to be important virulence factors in streptococcal infections. In the present study, a sag operon homologue was shown to be responsible for beta-hemolysis in S. anginosus strains by random gene knockout. Interestingly, contrary to pyogenic group streptococci, beta-hemolytic S. anginosus was shown to have two tandem sagA homologues, encoding streptolysin S (SLS)-like peptides, in the sag operon homologue. Gene deletion and complementation experiments revealed that both genes were functional, and these SLS-like peptides were essential for beta-hemolysis in beta-hemolytic S. anginosus. Furthermore, the amino acid sequence of these SLS-like peptides differed from that of the typical SLS of S. pyogenes, especially in their propeptide domain, and an amino acid residue indicated to be important for the cytolytic activity of SLS in S. pyogenes was deleted in both S. anginosus homologues. These data suggest that SLS-like peptides encoded by two sagA homologues in beta-hemolytic S. anginosus may be potential virulence factors with a different structure essential for hemolytic activity and/or the maturation process compared to the typical SLS present in pyogenic group streptococci. (Keyword) Streptococcus / Streptolysin S (Tokushima University Institutional Repository) ● Metadata: 117785 (Link to Publication Site) ● Publication site (DOI): 10.1128/JB.01344-12 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23292771 ● Search Scopus @ Elsevier (PMID): 23292771 ● Search Scopus @ Elsevier (DOI): 10.1128/JB.01344-12 (Tokushima University Institutional Repository: 117785, DOI: 10.1128/JB.01344-12, PubMed: 23292771)
40.	Toshifumi Tomoyasu, Atsushi Tabata, Yoko Ishikawa, Robert Whiley and Hideaki Nagamune : Small heat shock protein AgsA: an effective stabilizer of enzyme activities., Journal of Bioscience and Bioengineering, Vol.115, No.1, 15-19, 2013. (Summary) A small heat shock protein, AgsA, possesses chaperone activity that can reduce the amount of heat-aggregated protein in vivo, and suppress the aggregation of chemical- and heat-denatured proteins in vitro. Therefore, we examined the ability of AgsA to stabilize the activity of several enzymes by using this chaperone activity. We observed that AgsA can stabilize the enzymatic activities of Renilla (Renilla reniformis) luciferase, firefly (Photinus pyralis) luciferase, and β-galactosidase, and showed comparable or greater stabilization of these enzymes than bovine serum albumin (BSA), a well-known stabilizer of enzyme activities. In particular, AgsA revealed better stabilization of Renilla luciferase and β-galactosidase than BSA under disulfide bond-reducing conditions with dithiothreitol. In addition, AgsA also increased the enzymatic performance of β-galactosidase and various restriction enzymes to a comparable or greater extent than BSA. These data indicate that AgsA may be useful as a general stabilizer of enzyme activities. (Keyword) Small heat shock protein / HSP / enzyme (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jbiosc.2012.08.001 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22929984 ● Search Scopus @ Elsevier (PMID): 22929984 ● Search Scopus @ Elsevier (DOI): 10.1016/j.jbiosc.2012.08.001 (DOI: 10.1016/j.jbiosc.2012.08.001, PubMed: 22929984)
41.	Kazuto Ohkura, Yuki Kawaguchi, Atsushi Tabata, Atsushi Yamamoto, Yasuo Shinohara, Hideaki Nagamune and Hitoshi Hori : Molecular Profiles of Cholesterol-dependent Cytolysin Family-derived 11mer Regions, Anticancer Research, Vol.32, No.6, 2343-2346, 2012. (Summary) Cholesterol-dependent cytolysins (CDCs) are secreted from various types of bacteria and are involved in various diseases (e.g. abscess formation). Traditional CDCs has a conserved 11mer region, which is a key structure in membrane recognition. Based on the X-ray data of traditional CDC perfringolysin O (PFO), molecular models of intermedilysin (ILY), pyolysin (PLO), vaginolysin (VLY), and Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) were constructed. The 11mer regions of these models were extracted, and their molecular features were analyzed. The dipole moments of these 11mer regions were classified into four types, and their stereo-hydrophobicity (dGW) was different. It was thought that these results influenced the species specificity and membrane recognition of each cytolysin. Traditional CDCs, ILY, PLO, and VLY consisted of four domains (domains 1 to 4). Domain 0 existed on the N-terminal side in Sm-hPAF in addition to these four domains. The 11mer sequence of Sm-hPAF is the same as that of VLY, but Sm-hPAF has slightly different characteristics (e.g. species specificity, membrane recognition, cholesterol dependency) compared to VLY. Dynamic structure analysis of domain 0 might clarify these differences. (Keyword) Amino Acid Sequence / Cytotoxins / Humans / Models, Molecular / Molecular Sequence Data / X-Ray Diffraction (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22641672 ● Search Scopus @ Elsevier (PMID): 22641672 (PubMed: 22641672)
42.	Atsushi Tabata, Yukimasa Ohkubo, Eriko Sakakura, Toshifumi Tomoyasu, Kazuto Ohkura and Hideaki Nagamune : Investigation of a Bacterial Pore-forming Chimera Toxin for Application as a Novel Drug-delivery System Tool, Anticancer Research, Vol.32, No.6, 2323-2330, 2012. (Summary) Cholesterol-dependent cytolysins (CDCs) are pore-forming toxins from Gram-positive bacteria. The aim of this study was to investigate the potential of a CDC, intermedilysin, as a drug-delivery system (DDS) for clinical application. Intermedilysin was modified by the addition of a disulfide bridge to regulate pore formation, by swapping domain 4 to provide cholesterol-binding capacity, and by the introduction of a targeting domain. The resultant chimera protein, His-LTBP-CDC(ss)(IP), was investigated for its use as a DDS tool in vitro. His-LTBP-CDC(ss)(IP) exhibited a regulated pore-forming capacity under reducing conditions. This chimera protein was able to deliver a drug-carrier liposome specifically to the target cell, to be endocytosed into the cell with subsequent release of the components into the cytoplasm. A chimera protein derived from the bacterial pore-forming toxin intermedilysin (His-LTBP-CDC(ss)(IP)) forms the basis for a novel DDS tool. (Keyword) Bacteriocins / Cell Line, Tumor / Drug Delivery Systems / Electrophoresis, Polyacrylamide Gel / Humans / Liposomes / Microscopy, Fluorescence / Recombinant Fusion Proteins (Tokushima University Institutional Repository) ● Metadata: 117784 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22641669 ● Summary page in Scopus @ Elsevier: 2-s2.0-84864557140 (Tokushima University Institutional Repository: 117784, PubMed: 22641669, Elsevier: Scopus)
43.	Toshifumi Tomoyasu, Atsushi Tabata, Hidenori Imaki, Keigo Tsuruno, Aya Miyazaki, Kenji Sonomoto, Robert Whiley and Hideaki Nagamune : Role of Streptococcus intermedius DnaK chaperone system in stress tolerance and pathogenicity, Cell Stress & Chaperones, Vol.17, No.1, 41-55, 2012. (Summary) Streptococcus intermedius is a facultatively anaerobic, opportunistic pathogen that causes purulent infections and abscess formation. The DnaK chaperone system has been characterized in several pathogenic bacteria and seems to have important functions in stress resistance and pathogenicity. However, the role of DnaK in S. intermedius remains unclear. Therefore, we constructed a dnaK knockout mutant that exhibited slow growth, thermosensitivity, accumulation of GroEL in the cell, and reduced cytotoxicity to HepG2 cells. The level of secretion of a major pathogenic factor, intermedilysin, was not affected by dnaK mutation. We further examined the function and property of the S. intermedius DnaK chaperone system by using Escherichia coli ΔdnaK and ΔrpoH mutant strains. S. intermedius DnaK could not complement the thermosensitivity of E. coli ΔdnaK mutant. However, the intact S. intermedius DnaK chaperone system could complement the thermosensitivity and acid sensitivity of E. coli ΔdnaK mutant. The S. intermedius DnaK chaperone system could regulate the activity and stability of the heat shock transcription factor ς(32) in E. coli, although S. intermedius does not utilize ς(32) for heat shock transcription. The S. intermedius DnaK chaperone system was also able to efficiently eliminate the aggregated proteins from ΔrpoH mutant cells. Overall, our data showed that the S. intermedius DnaK chaperone system has important functions in quality control of cellular proteins but has less participation in the modulation of expression of pathogenic factors. (Keyword) Streptococci / molecular chaperone / 病原性 (Link to Publication Site) ● Publication site (DOI): 10.1007/s12192-011-0284-4 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21822788 ● Search Scopus @ Elsevier (PMID): 21822788 ● Search Scopus @ Elsevier (DOI): 10.1007/s12192-011-0284-4 (DOI: 10.1007/s12192-011-0284-4, PubMed: 21822788)
44.	Hisashi Ohkuni, Hideaki Nagamune, Nana Ozaki, Atsushi Tabata, Yuko Todome, Yukino Watanabe, Hidemi Takahashi, Kazuto Ohkura, Hiroki Kourai, Hiromi Ohtsuka, Vincent A. Fischetti and John B. Zabriskie : Characterization of recombinant Streptococcus mitis-derived human platelet aggregation factor, APMIS, Vol.120, No.1, 56-71, 2012. (Summary) We previously purified Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) from the culture supernatant of S. mitis strain Nm-65, isolated from the tooth surface of a patient with Kawasaki disease. Here we produced recombinant Sm-hPAF protein (rSm-hPAF) in Escherichia coli, to determine whether rSm-hPAF conserves its platelet aggregation activity. rSm-hPAF precursor (665 amino acids) shows up to 36-56% identity with the family of cholesterol-dependent cytolysins (CDCs), and rSm-hPAF displayed potent hemolytic activity toward mammalian erythrocytes, including human erythrocytes with platelet aggregation activity. The 162-amino acid amino-terminal domain of rSm-hPAF was found in no other CDCs except lectinolysin; this domain is homologous to a portion of pneumococcal fucolectin-related protein. Interestingly, suilysin (SLY) and pneumolysin (PLY) of CDCs also exhibit substantial human platelet aggregation activity, similar to rSm-hPAF, and the platelet aggregation by rSm-hPAF, SLY, and PLY was morphologically confirmed using light and electron microscopy. (Keyword) Adult / Amino Acid Sequence / Animals / Antibodies, Monoclonal / Bacterial Proteins / bacterial toxins / Base Sequence / Blood Platelets / Cell Adhesion Molecules / cholesterol / Cytotoxins / DNA, Bacterial / Female / Genes, Bacterial / Hemolysin Proteins / Humans / L-Lactate Dehydrogenase / Mice / Mice, Inbred BALB C / transmission electron microscopy / Molecular Sequence Data / Mucocutaneous Lymph Node Syndrome / Phylogeny / Platelet Aggregation / Recombinant Proteins / Sequence Homology, Amino Acid / Streptococcus mitis / Streptolysins (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1600-0463.2011.02813.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22151309 ● Search Scopus @ Elsevier (PMID): 22151309 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0463.2011.02813.x (DOI: 10.1111/j.1600-0463.2011.02813.x, PubMed: 22151309)
45.	Heni Susilowati, Hirohiko Okamura, Katsuhiko Hirota, Masayuki Shono, Kaya Yoshida, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : Intermedilysin induces EGR-1 expression through calcineurin/NFAT pathway in human cholangiocellular carcinoma cells., Biochemical and Biophysical Research Communications, Vol.404, No.1, 57-61, 2011. (Summary) (Intermedilysin (ILY) is a cholesterol-dependent cytolysin produced by Streptococcus intermedius, which is associated with human brain and liver abscesses. Although intrahepatic bile duct cells play a valuable role in the pathogenesis of liver abscess, the molecular mechanism of ILY-treated intrahepatic bile duct cells remains unknown. In this study, we report that ILY induced a nuclear accumulation of intracellular calcium ([Ca(2+)]i) in human cholangiocellular cells HuCCT1. We also demonstrate that 10 ng/ml ILY induced NFAT1 dephosphorylation and its nuclear translocation in HuCCT1 cells. In contrast to the result that ILY induced NF-B translocation in human hepatic HepG2 cells, ILY did not affect NF-B localization in HuCCT1 cells. Dephosphorylation and nuclear translocation of NFAT1 caused by ILY were prevented by [Ca(2+)]i calcium chelator, BAPTA/AM, and calcineurin inhibitors, cyclosporine A and tacrolimus. ILY induced early growth response-1 (EGR-1) expression and it was inhibited by the pre-treatment with cyclosporine A, indicating that the calcineurin/NFAT pathway was involved in EGR-1 expression in response to ILY. ILY-induced calcineurin/NFAT1 activation and sequential EGR-1 expression might be related to the pathogenesis of S. intermedius in human bile duct cells.) (Keyword) Bacteriocins / Bile Ducts, Intrahepatic / Calcineurin / calcium / Cell Line, Tumor / Cell Nucleus / Early Growth Response Protein 1 / Humans / NFATC Transcription Factors (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbrc.2010.11.057 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21094139 ● Summary page in Scopus @ Elsevier: 2-s2.0-78650872240 (DOI: 10.1016/j.bbrc.2010.11.057, PubMed: 21094139, Elsevier: Scopus)
46.	Ayuko Takao, Hideaki Nagamune and Nobuko Maeda : Sialidase of Streptococcus intermedius: a putative virulence factor modifying sugar chains, Microbiology and Immunology, Vol.54, No.10, 584-595, 2010. (Summary) A sialidase gene of Streptococcus intermedius was cloned. It was most similar to nanA, a major sialidase gene in Streptococcus pneumoniae, and was expressed in Escherichia coli. Since the gene-knockout S. intermedius strain lost detectable sialidase activity, the gene might code, either solely or mainly, the glycosidase in the bacterial genome. Polymerase chain reaction using the primers for the nanA homologue in S. intermedius (described as nanA below) showed that this sialidase gene was commonly distributed within the isolates of S. intermedius, but not found in the strains of other species among the anginosus group. In biofilm formation assay under cultivation with mucin, the nanA-deleted S. intermedius maintained the amount of biofilm for 72 hr, while that of the parent strain decreased during incubation from 24 to 72 hr. Since sialidase activity in the parent strain increased during that time period, sialidase might contribute to the degradation of biofilm under sialic acid-rich conditions. When S. intermedius was added into the HepG2 hepatoma culture, the calculated disassociation constant (K(d)) of EDTA-releasable bacterial adhesion to the cells was higher in the nanA-deleted strain than in the parent. Furthermore, the rate constant, assuming endocytosis of the bacterium mediated by ASGP-R in HepG2 cells, seemed to be increased by sialidase pretreatment of the bacterial cells before addition to the cell culture. According to the results, modification of sugar chains by sialidase on the bacterial surface and in the surrounding environment might influence both bacterial interaction and host-bacterial interaction in S. intermedius. (Keyword) Biofilms / Hep G2 Cells / Humans / Neuraminidase / Streptococcus intermedius / Virulence Factors (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1348-0421.2010.00257.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21118296 ● CiNii @ National Institute of Informatics (CRID): 1523388080672079872 ● Search Scopus @ Elsevier (PMID): 21118296 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1348-0421.2010.00257.x (DOI: 10.1111/j.1348-0421.2010.00257.x, PubMed: 21118296, CiNii: 1523388080672079872)
47.	Toshifumi Tomoyasu, Atsushi Tabata, Hiroshima Riki, Imaki Hidenori, Masuda Sachiko, Whiley A. Robert, Aduse-Opoku Joseph, Kikuchi Ken, Hiramatsu Keiichi and Hideaki Nagamune : Role of catabolite control protein A in the regulation of intermedilysin production by Streptococcus intermedius., Infection and Immunity, Vol.78, No.9, 4012-4021, 2010. (Summary) Streptococcus intermedius is an opportunistic pathogen of humans that causes purulent infections, including brain and liver abscesses. This pathogen secretes a human-specific cytolysin, intermedilysin, which has been recognized as a major virulence factor. However, most of the expressional control mechanisms of ily are still unknown. To determine these mechanisms, we analyzed the nucleotide sequence of the ily promoter region. We found a highly homologous region to the catabolite-repressible element (cre) in the ily promoter region and observed a considerable decrease in the amount of secreted intermedilysin when cells were grown in a culture medium containing high concentrations of glucose/utilizable carbohydrates. Disruption of the ccpA gene, which encodes catabolite control protein A, did not induce catabolite repression of ily by glucose/utilizable carbohydrates. In cre mutants, catabolite repression of ily was partially restored, and purified catabolite control protein A bound to an oligonucleotide containing the cre consensus sequence in the ily promoter region. In addition, a prolonged lag phase and slower doubling time of the ccpA mutant cells were observed. Our data show that S. intermedius can modulate ily expression and growth rate through catabolite control protein A-mediated monitoring of the extracellular glucose/utilizable carbohydrate concentration. (Keyword) virulence factor (Link to Publication Site) ● Publication site (DOI): 10.1128/IAI.00113-10 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20624907 ● Search Scopus @ Elsevier (PMID): 20624907 ● Search Scopus @ Elsevier (DOI): 10.1128/IAI.00113-10 (DOI: 10.1128/IAI.00113-10, PubMed: 20624907)
48.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Investigation of the chaperone function of the small heat shock protein AgsA., BMC Biochemistry, Vol.11, 27, 2010. (Summary) A small heat shock protein AgsA was originally isolated from Salmonella enterica serovar Typhimurium. We previously demonstrated that AgsA was an effective chaperone that could reduce the amount of heat-aggregated proteins in an Escherichia coli rpoH mutant. AgsA appeared to promote survival at lethal temperatures by cooperating with other chaperones in vivo. To investigate the aggregation prevention mechanisms of AgsA, we constructed N- or C-terminal truncated mutants and compared their properties with wild type AgsA. AgsA showed significant overall homology to wheat sHsp16.9 allowing its three-dimensional structure to be predicted. Truncations of AgsA until the N-terminal 23rd and C-terminal 11th amino acid (AA) from both termini preserved its in vivo chaperone activity. Temperature-controlled gel filtration chromatography showed that purified AgsA could maintain large oligomeric complexes up to 50 degrees C. Destabilization of oligomeric complexes was observed for N-terminal 11- and 17-AA truncated AgsA; C-terminal 11-AA truncated AgsA could not form large oligomeric complexes. AgsA prevented the aggregation of denatured lysozyme, malate dehydrogenase (MDH) and citrate synthase (CS) but did not prevent the aggregation of insulin at 25 degrees C. N-terminal 17-AA truncated AgsA showed no chaperone activity towards MDH. C-terminal 11-AA truncated AgsA showed weak or no chaperone activity towards lysozyme, MDH and CS although it prevented the aggregation of insulin at 25 degrees C. When the same amount of AgsA and C-terminal 11-AA truncated AgsA were mixed (half of respective amount required for efficient chaperone activities), good chaperone activity for all substrates and temperatures was observed. Detectable intermolecular exchanges between AgsA oligomers at 25 degrees C were not observed using fluorescence resonance energy transfer analysis; however, significant exchanges between AgsA oligomers and C-terminal truncated AgsA were observed at 25 degrees C. Our data demonstrate that AgsA has several regions necessary for efficient chaperone activity: region(s) important for lysozyme chaperone activity are located outer surface of the oligomeric complex while those region(s) important for insulin are located inside the oligomeric complex and those for MDH are located within the N-terminal arm. In addition, the equilibrium between the oligomer and the dimer structures appears to be important for its efficient chaperone activity. (Keyword) chaperone (Tokushima University Institutional Repository) ● Metadata: 114744 (Link to Publication Site) ● Publication site (DOI): 10.1186/1471-2091-11-27 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20653971 ● Search Scopus @ Elsevier (PMID): 20653971 ● Search Scopus @ Elsevier (DOI): 10.1186/1471-2091-11-27 (Tokushima University Institutional Repository: 114744, DOI: 10.1186/1471-2091-11-27, PubMed: 20653971)
49.	Ikuko Haruta, Ken Kikuchi, Etsuko Hashimoto, Minoru Nakamura, Hiroshi Miyakawa, Katsuhiko Hirota, Noriyuki Shibata, Hidehito Kato, Yutaka Arimura, Yoichiro Kato, Takehiko Uchiyama, Hideaki Nagamune, Makio Kobayashi, Yoichiro Miyake, Keiko Shiratori and Junji Yagi : Long-term bacterial exposure can trigger nonsuppurative destructive cholangitis associated with multifocal epithelial inflammation., Laboratory Investigation; a Journal of Technical Methods and Pathology, Vol.90, No.4, 577-588, 2010. (Summary) Bacterial infection has become a focus of attention in the pathogenesis of primary biliary cirrhosis (PBC). We earlier reported that the bacterial lipoteichoic acid was detected at the sites of inflammation around damaged bile ducts in the livers of PBC, and PBC patients' sera showed high titers against streptococcal histone-like protein. Here, we investigated whether chronic bacterial exposure could trigger PBC-like epithelial cell damage in normal mouse. BALB/c mice were repeatedly inoculated with various bacteria for 8 weeks. At 1 week (Group 1) and 3, 4, or 20 months (long term; Group 2) after the final inoculation, mice were killed to obtain samples. In the livers of the Streptococcus intermedius (S.i.)-inoculated mice in Group 1, cellular infiltration was predominantly observed around the bile ducts over the hepatic parenchyma. In the S.i.-inoculated mice in Group 2, portal but not parenchymal inflammation was observed in the livers, and periductal cellular infiltrates were detected in the salivary glands. Both S.i.-inoculated Groups 1 and 2 BALB/c mice sera had antibodies against HuCCT1 biliary epithelial cells, anti-nuclear antibodies, and anti-gp210 antibodies, but not anti-mitochondrial antibodies. Immunoreactivity to histone-like DNA-binding protein of S.i. (S.i.-HLP) was detectable around the sites of chronic nonsuppurative destructive cholangitis in the portal area in the livers of both S.i.-inoculated Groups 1 and 2 BALB/c mice. Furthermore, anti-S.i.-HLP antibody bound to synthetic gp210 peptide, as well. Bacteria triggered PBC-like cholangitis, multifocal epithelial inflammation, and autoantibody production. Bacteria are likely involved in the pathogenesis of PBC and of associated multifocal epithelial inflammation. (Keyword) Animals / Antibodies, Antinuclear / Disease Models, Animal / Epithelial Cells / Immunity, Innate / Inflammation / Liver Cirrhosis, Biliary / Mice / Mice, Inbred BALB C / Nuclear Pore Complex Proteins / Streptococcus intermedius (Link to Publication Site) ● Publication site (DOI): 10.1038/labinvest.2010.40 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20142809 ● Search Scopus @ Elsevier (PMID): 20142809 ● Search Scopus @ Elsevier (DOI): 10.1038/labinvest.2010.40 (DOI: 10.1038/labinvest.2010.40, PubMed: 20142809)
50.	Makoto Nishimoto, Tohru Morimitsu, Nobutake Tamai, Shoji Kaneshina, Hideaki Nagamune and Hitoshi Matsuki : Inhibition of anti-fluorescent probe monoclonal antibody by long-chain amphiphiles, Colloids and Surfaces B:Biointerfaces, Vol.75, No.1, 80-87, 2010. (Summary) Inhibitions of amphiphiles with a long chain (alcohols with carbon number of 10, 12, 14, 16 and the corresponding fatty acids) on the antigen-antibody reaction between fluorescent probe (fluorescein (FL)) and anti-fluorescent probe monoclonal antibody (immunoglobulin G (IgG)-49) were investigated by fluorescence spectroscopy. The resulting inhibition modes were compared with those of specific inhibitors, 5- and 6-aminofluoresceins (5- and 6-FLNH(2)). In the presence of all inhibitors, the fluorescence quenching of FL as the result of inhibitor binding to IgG-49 was hindered in an inhibitor concentration-dependent manner. The values of IC(50) and Hill number for the inhibitors were determined from dose-response curves of the quenching inhibition. The IC(50) values of the alcohols were much more dependent on the chain length than those of the fatty acids although all the values were in orders of microM. The Hill numbers of both kinds of inhibitors became larger values than 1, but they had different values for a series of alcohols and fatty acids, respectively. By contrast, the IC(50) values of 5- and 6-FLNH(2) were in orders of nM and the Hill numbers became smaller values than 1. Since the Lineweaver-Burk plots produced an intersection point on the intercept of the X-axis for the long-chain inhibitors with carbon number of 14 while did that on the intercept of the Y-axis for 6-FLNH(2), respectively, we judged the inhibition modes of the former as non-competitive ones and those of the latter as a competitive one. Further, the effect of pressure on the inhibition by C(14)OH, C(13)COOH and 5-FLNH(2) was examined, and the standard volume changes of IgG-49 for the inhibitor binding were calculated from the Johnson-Eyring plots. The volume change for 5-FLNH(2) was smaller than those for C(14)OH and C(13)COOH although they became all negative. This suggests that the volume of IgG-49 shrinks by the addition of these inhibitors, and IgG-49 is not relevant to a model protein for the molecular mechanism of anesthesia. (Keyword) Alcohols / Animals / Antibodies, Monoclonal / Fluorescein / Fluorescent Dyes / Inhibitory Concentration 50 / Kinetics / Ligands / Mice / Pressure (Link to Publication Site) ● Publication site (DOI): 10.1016/j.colsurfb.2009.08.012 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19740635 ● Search Scopus @ Elsevier (PMID): 19740635 ● Search Scopus @ Elsevier (DOI): 10.1016/j.colsurfb.2009.08.012 (DOI: 10.1016/j.colsurfb.2009.08.012, PubMed: 19740635)
51.	Shuntaro Ito, Hideaki Nagamune, Haruki Tamura and Yasuo Yoshida : Identification and molecular analysis of betaC-S lyase producing hydrogen sulfide in Streptococcus intermedius., Journal of Medical Microbiology, Vol.57, No.11, 1411-1419, 2008. (Summary) Hydrogen sulfide (H(2)S) is a toxic gas that induces the modification and release of haemoglobin in erythrocytes; however, it also functions in methionine biosynthesis in bacteria. betaC-S lyase, encoded by the lcd gene, is responsible for bacterial H(2)S production through the cleavage of l-cysteine. In this study, 26 of 29 crude extracts from reference and clinical strains of Streptococcus intermedius produced H(2)S from l-cysteine. The capacities in those strains were not higher than those in strains of the other anginosus group of streptococci, Streptococcus anginosus and Streptococcus constellatus, but were much greater than those in strains of Streptococcus gordonii, which is known to have an extremely low capacity for H(2)S production. Incubation of the remaining three extracts with l-cysteine did not result in H(2)S production. Sequence analysis revealed that the lcd genes from these three strains (S. intermedius strains ATCC 27335, IMU151 and IMU202) contained mutations or small deletions. H(2)S production in crude extracts prepared from S. intermedius ATCC 27335 was restored by repairing the lcd gene sequence in genomic DNA. The kinetic properties of the purified recombinant protein encoded by the repaired lcd gene were comparable to those of native proteins produced by H(2)S-producing strains, whereas the truncated protein produced by S. intermedius ATCC 27335 had no enzymic activity with l-cysteine or l-cystathionine. However, real-time PCR analysis indicated that the lcd gene in strains ATCC 27335, IMU151 and IMU202 is transcribed and regulated in a manner similar to that in the H(2)S-producing strain. (Keyword) Base Sequence / Carbon-Sulfur Lyases / Cystathionine / Cysteine / Hydrogen Sulfide / Molecular Sequence Data / Recombinant Proteins / Streptococcus intermedius (Link to Publication Site) ● Publication site (DOI): 10.1099/jmm.0.2008/001677-0 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18927421 ● Search Scopus @ Elsevier (PMID): 18927421 ● Search Scopus @ Elsevier (DOI): 10.1099/jmm.0.2008/001677-0 (DOI: 10.1099/jmm.0.2008/001677-0, PubMed: 18927421)
52.	Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Tsuneko Ono, Hideaki Nagamune, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake : The essentiality and involvement of Streptococcus intermedius histone-like DNA-binding protein in bacterial viability and normal growth, Molecular Microbiology, Vol.68, No.5, 1268-1282, 2008. (Summary) Streptococcus intermedius histone-like DNA-binding protein (Si-HLP) is a homodimeric protein and, conserved with Escherichia coli HU, a well-documented nucleoid-associated protein (NAP). In E. coli, HU plays important roles as both structural and regulatory factors, but it is not essential for E. coli viability. Streptococcal HLP has been found to bind host cells and induce cytokine production, but its physiological role remains poorly defined. In the present study, using gene insertion knockout and tetracycline-regulated antisense RNA expression techniques, we determined whether Si-HLP is essential for bacterial viability and normal growth in S. intermedius. The Si-HLP-downregulated S. intermedius strain showed alterations in its morphology and surface properties. Downregulation of Si-HLP led to an expanded nucleoid to fill the intracellular space. Transcription levels of several genes, including virulence-associated factors, were found to be activated or repressed in the antisense Si-hlp RNA-expressing strain by real-time PCR and reverse-transcription PCR. Collectively, these data suggest that Si-HLP serves as an essential NAP governing the nucleoid architecture and controlling the gene transcription profile in S. intermedius. (Keyword) Bacterial Proteins / DNA-Binding Proteins / Escherichia coli Proteins / Gene Expression Regulation, Bacterial / Histones / Microbial Viability / Streptococcus intermedius / Transcription, Genetic (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1365-2958.2008.06232.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18410499 ● Summary page in Scopus @ Elsevier: 2-s2.0-43749117518 (DOI: 10.1111/j.1365-2958.2008.06232.x, PubMed: 18410499, Elsevier: Scopus)
53.	Dali Liu, Hiromichi Yumoto, Katsuhiko Hirota, Keiji Murakami, Kanako Takahashi, Kouji Hirao, Takashi Matsuo, Kazuto Ohkura, Hideaki Nagamune and Yoichiro Miyake : Histone-like DNA binding protein of Streptococcus intermedius induces the expression of pro-inflammatory cytokines in human monocytes via action of ERK1/2 and JNK pathways, Cellular Microbiology, Vol.10, No.1, 262-276, 2008. (Summary) Streptococcus intermedius is a commensal associated with serious, deep-seated purulent infections in major organs, such as the brain and liver. Histone-like DNA binding protein (HLP) is an accessory architectural protein in a variety of bacterial cellular processes. In this study, we investigated the mechanisms of pro-inflammatory cytokine inductions in THP-1 cells by stimulation with recombinant HLP of S. intermedius (rSi-HLP). rSi-HLP stimulation-induced production of pro-inflammatory cytokines (IL-8, IL-1 beta and TNF-alpha) occurred in a time- and dose-dependent manner. In contrast with the heat-stable activity of DNA binding, the induction activity of rSi-HLP was heat-unstable. In subsequent studies, rSi-HLP acted cooperatively with lipoteichoic acid, the synthetic Toll-like receptor 2 agonist, Pam3CSK4, and the cytosolic nucleotide binding oligomerization domain 2 receptor agonist, muramyldipeptide. Furthermore, Western blot and blocking assays with specific inhibitors showed that rSi-HLP stimulation induced the activation of cell signal transduction pathways, extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinase (JNK). In addition to its physiological role in bacterial growth through DNA binding, these results indicate that Si-HLP can trigger a cascade of events that induce pro-inflammatory responses via ERK1/2 and JNK signal pathways, and suggest that bacterial HLP may contribute to the activation of host innate immunity during bacterial infection. (Keyword) Acetylmuramyl-Alanyl-Isoglutamine / Bacterial Proteins / Cell Line / Cytokines / DNA, Bacterial / DNA-Binding Proteins / Hot Temperature / Humans / JNK Mitogen-Activated Protein Kinases / Lipopolysaccharides / Mitogen-Activated Protein Kinase 3 / Molecular Sequence Data / Monocytes / Recombinant Proteins / Sequence Analysis, DNA / Signal Transduction / Streptococcus intermedius / Teichoic Acids / Up-Regulation (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1462-5822.2007.01040.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17883418 ● Search Scopus @ Elsevier (PMID): 17883418 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1462-5822.2007.01040.x (DOI: 10.1111/j.1462-5822.2007.01040.x, PubMed: 17883418)
54.	Akihiro Shirai, Takuya Maeda, Motoaki Ohkita, Hideaki Nagamune and Hiroki Kourai : Antibacterial activity and characteristics of modified ferrite powder coated with a gemini pyridinium salt molecule, Biocontrol Science, Vol.12, No.3, 83-91, 2007. (Summary) This report describes the synthesis of an antibacterial material consisting of a gemini quaternary ammonium salt (gemini-QUAT) immobilized on ferrite powder, and its antibacterial activity. A gemini-QUAT containing two pyridinium residues per molecule, 4,4'-[1,3-(2,2-dihydroxylmethyl-1,3-dithiapropane)]bis (1-octylpyridinium bromide), was immobilized on ferrite powder by a reaction between the hydroxyl group of the QUAT and trimethoxysilane. Immobilization of the gemini-QUAT on ferrite (F-gemini-QUAT) was confirmed when the dye, bromophenol blue, was released from F-gemini-QUAT-dye after contact between ferrite and the dye. Elemental analysis of the QUAT-ferrite determined the molar amount of QUAT on the ferrite. The antibacterial effect of the ferrite was investigated using a batch treatment system, and this effect was compared with that of another QUAT-ferrite (F-mono-QUAT) binding a mono-QUAT, which possesses one pyridinium residue, prepared by the same immobilization method as F-gemini-QUAT. Results indicated the F-gemini QUAT possessed a higher bactericidal potency and broader antibacterial spectrum compared to F-mono-QUAT. In addition, this study suggested that gemini-QUATs possessed high bactericidal potency without being influenced by immobilization to materials, and the antibacterial activity and characteristics of F-gemini-QUAT could be attributed to the unique structure of the immobilized gemini-QUAT. (Keyword) Anti-Bacterial Agents / Ferric Compounds / Magnetic Resonance Spectroscopy / Microbial Sensitivity Tests / Microscopy, Electron, Scanning / Pyridines / Quaternary Ammonium Compounds (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.12.83 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17927048 ● CiNii @ National Institute of Informatics (CRID): 1390282679441345152 ● Search Scopus @ Elsevier (PMID): 17927048 ● Search Scopus @ Elsevier (DOI): 10.4265/bio.12.83 (DOI: 10.4265/bio.12.83, PubMed: 17927048, CiNii: 1390282679441345152)
55.	Kachiko Sekiya, Takumi Akagi, Kiyoko Tatsuta, Eriko Sakakura, Tsutomu Hashikawa, Akio Abe and Hideaki Nagamune : Ultrastructural analysis of the membrane insertion of domain 3 of streptolysin O, Microbes and Infection, Vol.9, No.11, 1341-1350, 2007. (Summary) Streptolysin O (SLO) is a membrane-damaging toxic protein produced by group A streptococci. We performed an ultrastructural analysis of pore formation and the mechanism of hemolysis by SLO, using a mutant form of SLO [SLO(C/A)-SS] and native SLO. SLO(C/A)-SS was unable to penetrate the erythrocyte membrane as a consequence of immobilization that was due to a disulfide bond between domains. The SLO(C/A)-SS molecules that bound to membranes formed numerous single-layered ring-shaped structures that did not result in pores on the membranes. These structures were similar to the structures formed by native SLO at 0 degrees C. After treatment with dithiothreitol, SLO(C/A)-SS that had bound to membranes formed double-layered rings with pores on the membranes, as does native SLO at room temperature. Our morphological evidence demonstrates that an increase in temperature is necessary for the occurrence of conformational changes and for the formation of double-layered rings after the insertion of domain 3 into the host cell membrane. On the basis of a model of the oligomeric structure of SLO, we propose some new details of the mechanism of hemolysis by SLO. (Keyword) Animals / Bacterial Proteins / Cell Membrane / Erythrocytes / Humans / Microscopy, Energy-Filtering Transmission Electron / Models, Biological / Rabbits / Streptolysins / Temperature (Link to Publication Site) ● Publication site (DOI): 10.1016/j.micinf.2007.06.010 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17890127 ● Search Scopus @ Elsevier (PMID): 17890127 ● Search Scopus @ Elsevier (DOI): 10.1016/j.micinf.2007.06.010 (DOI: 10.1016/j.micinf.2007.06.010, PubMed: 17890127)
56.	Kazuto Ohkura, Hitoshi Hori and Hideaki Nagamune : Molecular dynamics of human-specific cytolysin: analysis of membrane binding motif for therapeutic application, Anticancer Research, Vol.26, No.6A, 4055-4062, 2006. (Summary) Intermedilysin (ILY) is a human-specific cytolysin secreted from Streptococcus intermedius. In this study, the dynamic structure of ILY, StreptolysinO (SLO) and their 12mer substituted mutants for 500 ps was analyzed. Several parameters, such as dipole moment and electrostatic potential, were determined to elucidate the molecular mechanism of membrane binding. Molecular models of lLY, SLO and their mutants were constructed using Insightll-Discover with the Homology module. Their molecular dynamics were simulated with the Discover3 (Insight module), and z-matrix data of the membrane-binding 12mer region were extracted to calculate the molecular orbital (MO) parameters (i.e., dipole moment, solvation free energy (dGW)). Cytolysins vibrated like a bow, and the dipole moment direction of ILY 12mer region was different from that of SLO. Certain ILY mutants indicated the SLO-like dipole properties, which had an SLO-type limer cysteine motif amino acid sequence. The ILY 11mer region was more hydrophobic than that of SLO, and seemed to interact easily with the cell membrane without cholesterol. The electrostatic potential field distribution of ILY differed from that of SLO, especially in the 11mer region. In the 11mer region, the dipole moment directions of these cytolysins were constant during molecular movement, and ready to interact with membrane components (i.e., cholesterol, phospholipid). (Keyword) Amino Acid Sequence / Animals / Bacterial Proteins / Bacteriocins / Cell Membrane / Computer Simulation / Erythrocytes / Hemolysis / Horses / Humans / Models, Molecular / Protein Binding / Rabbits / Sequence Alignment / Sheep / Static Electricity / Streptolysins / thermodynamics (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17195457 ● Summary page in Scopus @ Elsevier: 2-s2.0-33845923346 (PubMed: 17195457, Elsevier: Scopus)
57.	Tomoko Sumitomo, Akihiro Shirai, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Construction of a leftover bath water model for microbial testing, Biocontrol Science, Vol.11, No.3, 107-114, 2006. (Summary) In this study, in order to construct a model of leftover bath water, we analyzed one hundred samples of used bath water samples which were provided by twenty-eight volunteer families. It appeared that the number of detected bacteria from such bath water was correlated closely with the number of bathers. Moreover, the pH, acidity, chemical oxygen demand (COD), ion, protein content of the leftover bath water were measured. The number of bathers had no connection with the pH, acidity, COD, and ion content of the leftover bath water. However, the protein content of the bath water correlated with the number of detected bacteria. Based on these results, the model of leftover bath water was constructed. Achromobacter xylosoxidans, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa were incubated with the model bath water as indices of bath water contamination. The number of incubated viable cells in the model bath water increased with increasing concentrations of casamino acid. Consequently, it was suggested that varying the concentration of casamino acid based on family size or contamination would be necessary in the efficient use of the constructed model of leftover bath water for microbial testing. (Keyword) Baths / Colony Count, Microbial / Conservation of Natural Resources / Escherichia coli / Humans / Hydrogen-Ion Concentration / Klebsiella pneumoniae / Laundering / Microbiological Techniques / Models, Biological / Pseudomonas aeruginosa / Staphylococcus aureus / Staphylococcus epidermidis / Water / Water Microbiology (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.11.107 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17017128 ● Search Scopus @ Elsevier (PMID): 17017128 ● Search Scopus @ Elsevier (DOI): 10.4265/bio.11.107 (DOI: 10.4265/bio.11.107, PubMed: 17017128)
58.	Tomoko Sumitomo, Hideaki Nagamune, Takuya Maeda and Hiroki Kourai : Correlation between the Bacterioclastic Action of a Bis-quaternary Ammonium Compound and Outer Membrane Proteins, Biocontrol Science, Vol.11, No.3, 115-124, 2006. (Summary) Bis-quaternary ammonium compounds (bis-QACs) have the ability to cause a rapid and abundant leakage of the turbid materials from cells, and such a bacterioclastic ability leads to a potent bactericidal activity. In order to clarify the detailed mechanism of the bactericidal action of bis-QACs, the correlation between the bacterioclastic action of 4,4'-(1,6-hexamethylenedithio)bis(1-octylpyridinium bromide) (4DTBP-6,8) and the leakage of outer membrane pore protein E (OmpE) was investigated. Using the antiserum against a fusion protein consisting of GST and the OmpE protein of Escherichia coli encoded by the ompE gene, it was seen that the leakage of OmpE from E. coli cells was caused by treatment with low concentrations (much lower than the critical vesiculation concentration) of 4DTBP-6,8. Furthermore, it was confirmed that 4DTBP-6,8 caused an increase in the turbidity of the cell suspension of Klebsiella pneumoniae, Salmonella typhimurium and Serratia marcescences, and led to the leakage of several proteins which have a high percentage of homology with OmpE of E. coll. By immunoelectron microscopy investigation, it was revealed that the vesiculation from E. coli treated with 4DTBP-6,8 contains OmpE. In addition, the bacteriolytic action of 4DTBP-6,8 was investigated. The results suggested that the lysis of cells by bis-QACs was not an enzymatic action such as that by autolysin but a physical bacterioclastic action. Judging from these results, it is suggested that the leakage of OmpE is one of the major bacterioclastic actions of bis-QACs, and deals the bacterial cells a fatal blow. (Keyword) Anti-Bacterial Agents / Bacterial Outer Membrane Proteins / Base Sequence / DNA, Bacterial / Disinfectants / Escherichia coli / Escherichia coli Proteins / Microscopy, Immunoelectron / Quaternary Ammonium Compounds / Recombinant Fusion Proteins (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.11.115 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17017129 ● CiNii @ National Institute of Informatics (CRID): 1390282679441112960 ● Search Scopus @ Elsevier (PMID): 17017129 ● Search Scopus @ Elsevier (DOI): 10.4265/bio.11.115 (DOI: 10.4265/bio.11.115, PubMed: 17017129, CiNii: 1390282679441112960)
59.	Akihiro Shirai, Tomoko Sumitomo, Munehiro Yoshida, Tomoyo Kaimura, Hideaki Nagamune, Takuya Maeda and Hiroki Kourai : Synthesis and biological properties of gemini quaternary ammonium compounds,5,5'-[2,2'-(α,ω-polymethylnedicarbonyldioxy)diethyl]bis(3-alkyl-4-methylthiazolium iodide) and 5,5'- [2,2'-(ρ-phenylenedicarbonyldioxy)diethyl] bis(3-alkyl-4-methylthiazolium bromide), Chemical & Pharmaceutical Bulletin, Vol.54, No.5, 639-645, 2006. (Summary) We synthesized gemini quaternary ammonium compounds (gemini QACs) having two thiazolium moieties in a molecule, 5,5'-[2,2'-(alpha,omega-polymethylnedicarbonyldioxy)diethyl]bis(3-alkyl-4-methylthiazolium iodide) (5DEBT-m,n), on which the carbon number of the methylene chain linking the two thiazoles (m) is 2, 6 or 8 and that of the alkyl group (n) is 8, 10, 12, 14 or 16. 5,5'-[2,2'-(p-Phenylenedicarbonyldioxy)diethyl]bis(3-alkyl-4-methylthiazolium bromide) (5DEBT-P,n) was then synthesized, which is composed of a p-phenylene as the methylene spacer. For five gemini QAC series, in addition to the previously described 5DEBT-4,n to the four new compound series, their antimicrobial activities were determined. 5DEBT-m,10 and -P,10 exhibited a wide and strong bacteriostatic activity against gram-negative and -positive bacteria, fungi and then yeast in comparison with N-tetradecyl-5-(2-hydroxyethyl)-4-methylthiazolium iodide as a mono-QAC. The bactericidal activity of the 5DEBT series against Escherichia coli IFO 12713 and Staphylococcus aureus IFO 12732 was investigated on the basis of the effects of their alkyl chain length and their molecular hydrophobicity. It was found that the effect of theses factors on their activity significantly changes by the difference between the gram-negative and -positive bacteria. Although against the gram-negative bacterium, the change in the activity due to extension of the alkyl group for each compound affected the kind of methylene spacer, against the gram-positive bacterium, it was almost equal in spite of the methylene spacer. This result could be responsible for the bactericidal mechanism of the gemini QACs being influenced by the diversity of the steric structure participating in the methylene chain length and by the bacterium cell surface hydrophobicity. (Keyword) Alkylation / Anti-Bacterial Agents / Anti-Infective Agents / Antifungal Agents / Chemistry, Physical / Chromatography, Thin Layer / Escherichia coli / Fungi / Magnetic Resonance Spectroscopy / Microbial Sensitivity Tests / Physicochemical Phenomena / Quaternary Ammonium Compounds / Staphylococcus aureus / Structure-Activity Relationship / Thiazoles / Yeasts (Link to Publication Site) ● Publication site (DOI): 10.1248/cpb.54.639 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16651758 ● Search Scopus @ Elsevier (PMID): 16651758 ● Search Scopus @ Elsevier (DOI): 10.1248/cpb.54.639 (DOI: 10.1248/cpb.54.639, PubMed: 16651758)
60.	Hiroki Kourai, Tadao Yabuhara, Akihiro Shirai, Takuya Maeda and Hideaki Nagamune : Syntheses and antimicrobial activities of series of new bis-quaternary ammonium compounds, European Journal of Medicinal Chemistry, Vol.41, No.4, 437-444, 2006. (Summary) A series of new bis-quaternary ammonium compounds (bis-QACs), 4,4'-(2,9-dioxadecane) bis(1-alkylpyridinium bromide)s, 3,3'-(2,9-dioxadecane)bis(1-alkylpyridinium bromide)s and 3,3'-(2,7-dioxaoctane)bis(1-decylpyridinium bromide) was synthesized. The compounds were evaluated by their activities against bacteria, molds and yeasts; the activities were expressed as the minimum inhibitory concentrations (MIC) and/or the minimum bactericidal concentrations (MBC). Compound 4,4'-(2,9-dioxadecane) bis(1-decylpyridinium bromide) had MIC values which exceeded those of benzalkoniumchloride and thiabendazole. It was in vitro active against a broad spectrum of Gram-negative and Gram-positive bacteria, against yeasts and some molds, it had high solubility and thermal decomposition temperature. The relationships between structure and biological activity of the tested bis-QACs are discussed. (Keyword) Anti-Bacterial Agents / Antifungal Agents / Bacteria / Chemistry, Physical / Fungi / Hemolysis / Humans / Hydrogen-Ion Concentration / Indicators and Reagents / Magnetic Resonance Spectroscopy / Microbial Sensitivity Tests / Physicochemical Phenomena / Quaternary Ammonium Compounds / Structure-Activity Relationship / Temperature (Link to Publication Site) ● Publication site (DOI): 10.1016/j.ejmech.2005.10.021 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16517025 ● Search Scopus @ Elsevier (PMID): 16517025 ● Search Scopus @ Elsevier (DOI): 10.1016/j.ejmech.2005.10.021 (DOI: 10.1016/j.ejmech.2005.10.021, PubMed: 16517025)
61.	Kiyo Okazaki, Munehiro Yoshida, Mari Mayama, Akihiro Shirai, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Antifungal characteristics of N,N'-hexamethylenebis (4-carbamoyl-1-decylpyridinium bromide), Biocontrol Science, Vol.11, No.1, 37-42, 2006. (Summary) A bis-quaternary ammonium compound (bis-QAC), N,N'-hexamethylenebis(4-carbamoyl-1decylpyridinium bromide) (D-38), exhibited a wide antifungal spectrum and a strong activity against sixteen strains of fungi including nine strains isolated from various kinds of food. The antifungal activity was higher than that of N-dodecylpyridinium Iodide, a typical mono-QAC, and that of the commonly used fungicide, 2-(4-thiazolyl) benzimidazole. The activity of D-38, however, was comparatively low when it was measured by the agar dilution method. It is considered that the hydrophobic D-38 molecules, having two long alkyl chains, interacted with the agar medium, and therefore showed lower activity results. On the other hand, the activity of D-38 against Aspergillus niger IFO 6342 was not significantly influenced by temperature, pH and the initial spore concentration. These results indicate that bis-QACs can exhibit a high antifungal activity whether or not the environmental conditions change. (Keyword) Quaternary ammonium compound / Antifungal activity / N,N'-Hexamethylenebis (4-carbamoyl-1-decylpyridinium bromide) (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.11.37 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16637437 ● CiNii @ National Institute of Informatics (CRID): 1390001204465013632 ● Search Scopus @ Elsevier (PMID): 16637437 ● Search Scopus @ Elsevier (DOI): 10.4265/bio.11.37 (DOI: 10.4265/bio.11.37, PubMed: 16637437, CiNii: 1390001204465013632)
62.	Takuya Maeda, Shingo Tanaka, Hideaki Nagamune and Hiroki Kourai : Characterization and Protein Analysis of Escherichia coli Resistant to a Quaternary Ammonium Compound, Biocontrol Science, Vol.10, No.4, 139-145, 2005. (Keyword) Quaternary ammonium compound / Escherichia coli / Biocide resistance / Cell surface hydrophobicity / Efflux pump / Stress response (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.10.139 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001204465438592 ● Summary page in Scopus @ Elsevier: 2-s2.0-28944454279 (DOI: 10.4265/bio.10.139, CiNii: 1390001204465438592, Elsevier: Scopus)
63.	Akiko Sukeno, Hideaki Nagamune, Robert A. Whiley, Syed I. Jafar, Joseph Aduse-Opoku, Kazuto Ohkura, Takuya Maeda, Katsuhiko Hirota, Yoichiro Miyake and Hiroki Kourai : Intermedilysin Is Essential for the Invasion of Hepatoma HepG2 Cells by Streptococcus intermedius, Microbiology and Immunology, Vol.49, No.7, 681-694, 2005. (Summary) Streptococcus intermedius causes endogenous infections leading to abscesses. This species produces intermedilysin (ILY), a human-specific cytolysin. Because of the significant correlation between higher ILY production levels by S. intermedius and deep-seated abscesses, we constructed ily knockout mutant UNS38 B3 and complementation strain UNS38 B3R1 in order to investigate the role of ILY in deep-seated infections. Strain UNS38 reduced the viability of human liver cell line HepG2 at infection but not of rat liver cell line BRL3A. Isogenic mutant strain UNS38 B3 was not cytotoxic in either cell line. Quantification of S. intermedius revealed that in infected HepG2 cells UNS38 but not UNS38 B3 increased intracellularly concomitantly with increasing cell damage. This difference between UNS38 and UNS38 B3 was not observed with UNS38 B3R1. Invasion and proliferation in BRL3A cells was not observed. Masking UNS38 or UNS38 B3R1 with ILY antibody drastically decreased adherence and invasion of HepG2. Moreover, coating strain UNS38 B3 with ILY partially restored adherence to HepG2 but without subsequent bacterial growth. At 1 day post-infection, many intact UNS38 were detected in the damaged phagosomes of HepG2 with bacterial proliferation observed in the cytoplasm of dead HepG2 after an additional 2 day incubation. These results indicate that surface-bound ILY on S. intermedius is an important factor for invasion of human cells by this bacterium and that secretion of ILY within host cells is essential for subsequent host cell death. These data strongly implicate ILY as an important factor in the pathogenesis of abscesses in vivo by this streptococcus. (Keyword) Animals / Bacterial Proteins / Bacterial Toxins / Bacteriocins / Carcinoma, Hepatocellular / Cytotoxins / Humans / Streptococcal Infections / Streptococcus intermedius / Tumor Cells, Cultured (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16034212 ● Search Scopus @ Elsevier (PMID): 16034212 (PubMed: 16034212)
64.	Hitoshi Matsuki, Hideaki Nagamune, Shoji Kaneshina and Issaku Ueda : Inhibition modes of amphiphilic ligands with a long chain on the anti-fluorescent probe monoclonal antibody, Anesthesia and Resuscitation, Vol.41, No.1, 35-38, 2005. (Summary) 蛍光プローブフルオレセインとその特異的モノクローナル抗体との抗原抗体反応におよぼす長鎖両親媒性リガンド(炭素数10，12，14，16のアルコールおよび対応する脂肪酸)の作用様式を蛍光分光法で調べた．消光阻害の用量応答曲線はアルコール系列および脂肪酸系列でそれぞれ異なり，両リガンドの阻害様式の違いが示唆された．また，上記リガンドの阻害反応に対する圧力効果を調べ，両リガンド存在下ではフルオレセイン特異的モノクローナル抗体の体積は収縮することがわかった． (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-71249137791 (Elsevier: Scopus)
65.	Kazuto Ohkura, Akiko Sukeno, Hideaki Nagamune and Hiroki Kourai : Bridge-linked bis-quaternary ammonium anti-microbial agents: relationship between cytotoxicity and anti-bacterial activity of 5,5'-[2,2'-(tetramethylenedicarbonyldioxy)diethyl]bis(3-alkyl-4-methylthiazonium iodide)s, Bioorganic & Medicinal Chemistry, Vol.13, No.7, 2579-2587, 2005. (Summary) 論文題目:架橋構造を有するビス型第四運アンモニウム塩抗菌剤，5,5'-[2,2'-(tetramethylenedicarbonyldioxy)diethyl]bis(3-alkyl-4-methylthiazonium iodide)sの抗細菌活性と細胞毒性の相関，5種のビス型化合物について，構造と抗菌，毒性の相関を検討．anti-ester型5DEBT-4,8のコンフォメーション解析から1144個のコンフォーマを得，9サンプルの立体疎水性度を決定したところ他の化合物では極小点が1つであるのに対して，2つの極小点が存在した．これから5DEBT-4,8には疎水性度の異なる2群のコンフォーマが存在し高効率で低毒性なバランスとれた性質の要因と考えられた． (Keyword) Anti-Bacterial Agents / Cell Line / Cell Survival / Erythrocytes / Escherichia coli / Fibroblasts / Humans / Microbial Sensitivity Tests / Models, Molecular / Molecular Structure / Quaternary Ammonium Compounds / Structure-Activity Relationship (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bmc.2005.01.030 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15755659 ● Search Scopus @ Elsevier (PMID): 15755659 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bmc.2005.01.030 (DOI: 10.1016/j.bmc.2005.01.030, PubMed: 15755659)
66.	Akihiro Shirai, Takuya Maeda, Hideaki Nagamune, Hitoshi Matsuki, Shoji Kaneshina and Hiroki Kourai : Biological and physicochemical properties of gemini quaternary ammonium compounds in which the positions of a cross-linking sulfur in the spacer differ, European Journal of Medicinal Chemistry, Vol.40, No.1, 113-123, 2005. (Summary) Gemini型第四アンモニウム化合物(gemini QAC)の架橋構造のスルフィド結合の位置に着目した．メチレンスルフィド，エチレンジスルフィド，スルフィドがピリジン環に連結したgemini QACの3種のを合成し，抗菌活性，ヒト赤血球溶血毒性，界面活性能に関するスルフィド結合位置の影響について検討した．ピリジン環に連結したスルフィド結合を有する化合物は，抗菌力，溶血毒性，界面活性能いずれにおいても，他の2種薬剤よりも高いことが分かった．その強い抗菌力と溶血毒性は，スルフィド結合の共鳴効果によるアンモニウムカチオン電子密度の上昇，スルフィドがテトラメチレンスペーサ鎖両末端に存在することによるスペーサ鎖の高い伸縮性に起因した小さい分子占有面積，そして高い薬剤分子疎水性に寄与すると考え，スペーサ鎖の立体構造に影響し，スルフィドの位置がアンモニウムカチオン電子密度に影響を及ぼすと結論した． (Keyword) Anti-Bacterial Agents / Bacteria / Hemolysis / Humans / Hydrophobic and Hydrophilic Interactions / Microbial Sensitivity Tests / Quaternary Ammonium Compounds / Structure-Activity Relationship / Sulfides / Surface Tension (Link to Publication Site) ● Publication site (DOI): 10.1016/j.ejmech.2004.09.015 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15642416 ● Search Scopus @ Elsevier (PMID): 15642416 ● Search Scopus @ Elsevier (DOI): 10.1016/j.ejmech.2004.09.015 (DOI: 10.1016/j.ejmech.2004.09.015, PubMed: 15642416)
67.	Tadao Yabuhara, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Synthesis and Antimicrobial Characteristics of a Novel Biocide,4,4'-(1,6-Dioxyhexamethylene)bis-(1-alkylpyridinium halide), Biocontrol Science, Vol.9, No.4, 95-103, 2004. (Summary) We synthesized a new bis-quaternary ammonium compound (bis-QAC), 4, 4'-(1, 6-dioxyhexamethylene) bis (1-alkylpyridinium halide) s (4DOBP-6-n-X) (alkyl chain length, n=6, 8, 10, 12, 14, 16 and 18, X=Br or I) that has a symmetrical dimeric structure, and is composed of two alkylpyridinium halides connected with a dioxyhexamethylene. We examined the relationship of the hydrophobicity and antimicrobial activity using 4DOBP-6-n-X. The influences of pH, temperature, and hemolytic activity were then measured using 4, 4'-(1, 6-dioxyhexamethylene) bis (octylpyridinium bromide) (4DOBP-6-8-Br) which showed a superior antimicrobial activity among the 4DOBP-6-n-X series. The following three chemical compounds were used as a comparison: 4, 4'-(1, 6-dithiohexamethylene) bis (octylpyridinium bromide) (4DTBP-6-8-Br) which is considered as an excellent antimicrobial agent based on a previous report, alkylbenzyldimethyl ammonium chloride (BAC) and 2-(4'-thiazolyl) benzimida-zole (TBZ). In the results of the experiment, 4DOBP-6-8-Br exhibited a wide antimicrobial spectrum, and had a strong activity against bacteria and fungi comparable to 4DTBP-6-8-Br. The hemolytic activities of 4DOBP-6-8-Br and 4DTBP-6-8-Br were almost same, and lower than that of BAC. 4DTBP-6-8-Br had some defects in that it colored metals or generated a bad smell and a hazardous gas; however, in 4DOBP-6-n-X, these disadvantages were not present. 4DOBP-6-n-X seems to be an environmentally friendly antibacterial agent. (Keyword) Quaternary ammonium compound / Novel biocide / Antimicrobial activity / 4, 4'-(1, 6-dioxyhexamethylene)bis(1-alkylpyridinium halide) (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.9.95 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001204464915072 ● Summary page in Scopus @ Elsevier: 2-s2.0-13244286699 (DOI: 10.4265/bio.9.95, CiNii: 1390001204464915072, Elsevier: Scopus)
68.	Zhou Fei, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Synthesis and antimicrobial characteristics of novel biocides, 1,1'-(decanedioyl)bis(4-methyl-4-alkylpiperadinium iodide)s with a gemini structure, Biocontrol Science, Vol.9, No.3, 61-67, 2004. (Summary) 論文題目:ジェミニ構造を有する新規殺菌剤，1,1-(デカンジオイル)ビス(4-メチル-4-アルキルピペラジニウムアイオダイド)の合成と抗菌特性．ポリメチレンの両端にカルバモイル結合により2分子のピペラジニウムを導入した特徴的構造を有する薬剤の合成方法を確立し，薬剤の殺菌特性について検討した．さらに，pH，温度およびヒト赤血球に対する溶血特性を明らかにした．一連の合成薬剤の大腸菌に対する殺菌活性について薬剤分子疎水性を用いて構造活性相関の手法により解析したところ，ジェミニ型ピリジニウムとは異なる特徴を持っていることを明確にした． (Keyword) Quaternary ammonium compound / Gemini structure / Antimicrobial activity / 1, 1'-(Decanedioyl)bis(4-methyl-4-alkylpiperazinium iodide)s (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.9.61 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282679442271616 ● Summary page in Scopus @ Elsevier: 2-s2.0-7044238405 (DOI: 10.4265/bio.9.61, CiNii: 1390282679442271616, Elsevier: Scopus)
69.	Hideaki Nagamune, Kazuto Ohkura, Kazunori Umezu, Hidekatsu Shouji and Hiroki Kourai : A cell membrane modification technique using domain 4 of intermedilysin for immunotherapy against cancer, Anticancer Research, Vol.24, No.5, 3367-3372, 2004. (Summary) ヒト特異的細胞溶解毒素インターメディリシン(ILY)は，そのドメイン4(4D)だけでは細胞膜に強く結合するものの細胞毒性を示さない．この性質を応用し，4DのN末端に導入したCys残基のチオール基や4D分子表面のアミノ基を足場にして胎児性癌抗原(CEA)特異的な抗体の(Fab')2断片を連結した架橋体を作成し，CEA陽性癌細胞に対して活性化リンパ球等の細胞をターゲティングできるモジュールを開発した．この細胞膜機能の改変技術は細胞ターゲティング以外にも様々な利用法が期待できる． (Keyword) Animals / Antibodies, Monoclonal / Bacterial Proteins / Bacteriocins / Carcinoembryonic Antigen / Carcinoma, Medullary / Cell Line, Tumor / Cell Membrane / Cytotoxins / Erythrocyte Membrane / Erythrocytes / Humans / Immunoconjugates / Immunoglobulin Fragments / Immunotherapy / Protein Structure, Tertiary / Rabbits / Recombinant Proteins / Thyroid Neoplasms (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15515433 ● Search Scopus @ Elsevier (PMID): 15515433 (PubMed: 15515433)
70.	Kazuto Ohkura, Hideaki Nagamune and Hiroki Kourai : Structural analysis of human specific cytolysin intermedilysin aiming application to cancer immunotherapy, Anticancer Research, Vol.24, No.5C, 3343-3354, 2004. (Summary) ヒト特異的細胞溶解毒素インターメディリシン(ILY)を癌免疫療法に応用するため，ILYが属する毒素群(CBC)の一種，パーフリンゴリシンOの結晶構造を基にILYの立体構造を分子モデリングした．その結果，ILYは他のCBCと同様のドメイン構成を持ち，ドメイン4はドメイン1-3から立体的に独立していることや，ILYの11アミン酸領域は物理化学的な性質が他のCBCの場合と異なり，コレステロールを受け入れにくい構造であることが示された． (Keyword) Amino Acid Sequence / Bacterial Proteins / Bacterial Toxins / Bacteriocins / Crystallography, X-Ray / Cytotoxins / Erythrocyte Membrane / Hemolysin Proteins / Humans / Immunotherapy / Models, Molecular / Molecular Sequence Data / Protein Conformation / Protein Structure, Tertiary / Sequence Alignment (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15515430 ● Search Scopus @ Elsevier (PMID): 15515430 (PubMed: 15515430)
71.	Hideaki Nagamune, Kazuto Ohkura, Akiko Sukeno, Graeme Cowan, Timothy J. Mitchell, Wataru Ito, Ooki Ohnishi, Kanako Hattori, Miki Yamato, Katsuhiko Hirota, Yoichiro Miyake, Takuya Maeda and Hiroki Kourai : The human-specific action of intermedilysin, a homolog of streptolysin O, is dictated by domain 4 of the protein., Microbiology and Immunology, Vol.48, No.9, 677-692, 2004. (Summary) ヒト特異的細胞溶解毒素インターメディリシン(ILY)はストレプトリシンO(SLO)と同様にコレステロール(CHL)結合性細胞溶解毒素遺伝子ファミリーに属するが，CHLとの直接結合能を持たず，そのドメイン4でヒト細胞と結合することを明らかとした．またILYとSLO等の同属毒素間で多数のキメラ毒素を作成してその活性を調べた結果，SLO等ではCHLを結合する11アミノ酸領域は直接ヒト細胞の認識には関わらず，ここを除くドメイン4のC末端側56アミノ酸残基中にヒト細胞の受容体を認識する部位があることを示した． (Keyword) Amino Acid Sequence / Animals / Bacterial Proteins / Bacteriocins / Binding Sites / Cholesterol / Erythrocyte Membrane / Hemolysis / Humans / Liposomes / Microscopy, Electron, Transmission / Models, Molecular / Molecular Sequence Data / Point Mutation / Rabbits / Species Specificity / Streptococcus intermedius / Streptolysins (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15383705 ● CiNii @ National Institute of Informatics (CRID): 1521417754879669632 ● Search Scopus @ Elsevier (PMID): 15383705 (PubMed: 15383705, CiNii: 1521417754879669632)
72.	Ryoko Buma, Takuya Maeda, Yuko Marutaka, Masaharu Kamei, Hideaki Nagamune and Hiroki Kourai : Vectorial capacity of larvae pupae and adult of housefly (Musca domestica) for Escherichia coli 0157:H7 and the possibility of transmission from source to human, Medical Entomology and Zoology, Vol.55, No.2, 95-106, 2004. (Summary) イエバエが腸管出血性大腸菌O157:H7(O157)のベクターとなる可能性を検討するため，O157をイエバエの幼虫に食餌とともに与え，その幼虫及び蛹中での検出を行った．しかし腸内細菌は変態の間に減少する傾向を示し，羽化した成虫の32.6%は腸内細菌を保有していたがO157は検出されず，幼虫や蛹はO157伝搬にあまり寄与しないと考えられた．一方，24時間の伝搬実験の結果から，成虫は食品由来のO157を伝搬して食品を汚染することが示された．また分子疫学的な解析からも，イエバエ由来の株と食中毒患者からの分離株は非常に近縁の株であることが判明した．このように，ヒトでのO157感染にはイエバエが重要なベクターとなることを実証した． (Link to Publication Site) ● Publication site (DOI): 10.7601/mez.55.95 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.7601/mez.55.95 (DOI: 10.7601/mez.55.95)
73.	Tomoko Sumitomo, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Bacterioclastic Action of a Bis-Quaternary Ammonium Compound against Escherichia coli, Biocontrol Science, Vol.9, No.1,2, 1-9, 2004. (Summary) ビス型第四アンモニウム塩系化合物(bis-QAC)の大腸菌に対する細胞破壊作用について検討した．bis-QAC処理菌体からの濁り物質の増加とともにbis-QACの殺菌活性も上昇したことから，この濁り物質の生成はbis-QACの殺菌機構を解明する上で重要である．bis-QAC処理菌体から漏洩した物質の解析及び処理菌体の表面と内部の形態を電子顕微鏡で観察した結果，薬剤と細菌表層に存在するマグネシウムイオンとの置換反応により細胞膜内に侵入したbis-QACは，細胞表面にブレッブ形成を引き起こす．電子伝達系の呼吸系酵素を阻害し，リポポリサッカライドやポーリンタンパクのような細胞膜構成成分を漏洩させることが示唆された．細胞内部まで侵入したbis-QACがペプチドグリカンを破壊し，細胞表面に形成した大きなホールから細胞内物質を漏洩させることが細菌に対する致命的攻撃であることが示唆された． (Keyword) Quaternary ammonium compound / Bacterioclastic action / Critical vesiculation concentration (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.9.1 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282679441025280 ● Search Scopus @ Elsevier (DOI): 10.4265/bio.9.1 (DOI: 10.4265/bio.9.1, CiNii: 1390282679441025280)
74.	Ayuko Takao, Hideaki Nagamune and Nobuko Maeda : Identification of the anginosus group within the genus Streptococcus using polymerase chain reaction, FEMS Microbiology Letters, Vol.233, No.1, 83-89, 2004. (Summary) ヒト特異的細胞溶解毒素インターメディリシン遺伝子をS. intermedius菌種マーカーとし，またアンギノーサス群連鎖球菌に分布する2タイプのヒアルロニダーゼ遺伝子とrRNA遺伝子をそれぞれ組み合わせた2段階のPCRシステムを開発した．これを用いると現在知られているアンギノーサス群連鎖球菌の全菌種(3菌種，4亜菌種)を迅速に鑑別でき，本菌群による感染症の臨床診断にも応用可能であることを示した． (Keyword) the anginosus streptococci / polymerase chain reaction / species identification / MILLERI GROUP / GENOME SEQUENCE / INTERMEDIUS / CONSTELLATUS / PATHOGEN / GENES (Link to Publication Site) ● Publication site (DOI): 10.1016/j.femsle.2004.01.042 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1573387451555435008 ● Search Scopus @ Elsevier (DOI): 10.1016/j.femsle.2004.01.042 (DOI: 10.1016/j.femsle.2004.01.042, CiNii: 1573387451555435008)
75.	Fei Zhou, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Synthesis and Antimicrobial Characteristics of Novel Antimicrobial Agent,1,4-Dialky-1,4-dihydroxyethylpiperadinium Diiodides Immobilized on Inorganic Materials, Biocontrol Science, Vol.8, No.4, 167-175, 2003. (Summary) 論文題目:新規殺菌剤1,4-ジヒドロキシエチルピペラジニウムジアイオダイドを無機材料表面に固定化した材料の合成と抗菌特性本論文は固定化殺菌剤に関するものであり，分子内にアンモニウム残基とヒドロキシエチル基をそれぞれ2個導入した薬剤の新規合成研究およびシランカップリング反応によるフェライト表面およびガラスビーズ表面に固定化する研究およびこれらを用いて各種微生物に対する殺菌作用と殺菌特性を解析したものである．固定化殺菌材の殺菌活性はpHには影響を受けなかったが温度には大きな影響を受けることを明らかにした．しかし固定化殺菌材は非常に広い殺菌スペクトルを有し，5∼6回繰り返し使用には殺菌活性が低下しない優れた特徴を示した． (Keyword) Quaternary ammonium compound / Immobilized antibacterial agent / Antimicrobial activity (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.8.167 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282679441319168 ● Summary page in Scopus @ Elsevier: 2-s2.0-0348048693 (DOI: 10.4265/bio.8.167, CiNii: 1390282679441319168, Elsevier: Scopus)
76.	Akihiro Shirai, Takuya Maeda, Ichiro Hara, Azumi Yoshinari, Hideaki Nagamune and Hiroki Kourai : Antimicrobial characteristics of bis-quaternary ammonium compounds possessing a p-phenylene group in their spacer chains, Biocontrol Science, Vol.8, No.4, 151--157, 2003. (Summary) p-フェニレンスペーサにより連結された2つのピリジニウム塩から成るフェニレン-ビス型四アンモニウム化合物 (Ph-bis-QACs)の抗菌特性および構造活性相関についてメチレンスペーサ鎖により連結されたメチレン-ビス型四アンモニウム化合物(M-bis-QACs)と比較検討した．Ph-bis-QACsは，高い殺菌活性と広い抗菌スペクトルを示した．Ph-bis-QACsの抗菌活性は，M-bis-QACsのメチレンスペーサ鎖をp-フェニレンスペーサ鎖に変換することによってM-bis-QACsの活性より高くなった．M-bis-QACsの活性は，アンモニウム窒素の電子密度に影響を受けるが，Ph-bis-QACsはそれに依存しないことが分かった．従って，bis-QACの抗菌活性は，アンモニウム窒素の電子密度よりもスペーサ鎖の回転軸を減少させること，分子構造の剛直性を上昇させることによって，より強化されると考えられ，bis-QACの立体構造が，bis-QACの抗菌活性の重要な因子であると示唆した． (Keyword) Bis-quaternary ammonium compound / Antimicrobial activity / Structure-activity relationship (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.8.151 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001204464609920 ● Summary page in Scopus @ Elsevier: 2-s2.0-0346787725 (DOI: 10.4265/bio.8.151, CiNii: 1390001204464609920, Elsevier: Scopus)
77.	Tomoko Sumitomo, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Kinetic Analysis of the Bactericidal Action of a Bis-quaternary Ammonium Compound against Escherichia coli, Biocontrol Science, Vol.8, No.4, 145-149, 2003. (Summary) ビス型第四アンモニウム塩系化合物(bis-QAC)の大腸菌に対する殺菌機構を解明するためbis-QAC，4,4'-(1,6-ヘキサメチレンジチオ)ビス(1-オクチルピリジニウムブロマイド)の殺菌作用の動力学的特性を解析し，mono-QAC，N-オクチルピリジニウムブロマイドと比較した．mono-QACのアレニウスプロットは薬剤処理温度及び細胞培養温度の影響を受けて二つの直線を示したのに対し，bis-QACのアレニウスプロットは直線性を示した．mono-QACの見かけの殺菌活性化エネルギーは10-30℃で99.8kJ/mol，30-40℃で40.6kJ/molであったが，bis-QACは15.6kJ/molと非常に低い値であった．この結果より，bis-QACの殺菌作用はmono-QACとは異なるメカニズムであり，mono-QACの殺菌作用の律速段階が細菌細胞膜への作用であるのに対して，bis-QACは細胞膜表面への吸着であることが示唆された． (Keyword) Quaternary ammonium compound / Bactericidal action / Rate constant / Arrhenius plots / Activation energy (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.8.145 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282679441320576 ● Summary page in Scopus @ Elsevier: 2-s2.0-0346787723 (DOI: 10.4265/bio.8.145, CiNii: 1390282679441320576, Elsevier: Scopus)
78.	Atsushi Tabata, Hideaki Nagamune, Takuya Maeda, Keiji Murakami, Yoichiro Miyake and Hiroki Kourai : Correlation between resistance of Pseudomonas aeruginosa to quaternary ammonium compounds and expression of outer membrane protein OprR, Antimicrobial Agents and Chemotherapy, Vol.47, No.7, 2093-2099, 2003. (Summary) 論文題目:緑膿菌の細胞膜タンパク質OprRの発現と第四アンモニウム塩に対する耐性化の相関第四アンモニウム塩系消毒剤に耐性化した緑膿菌において顕著に発現量が増加する約25.6kDaの蛋白質を2次元電気泳動で分離し，そのN末端アミノ酸配列の解析によって，それが外膜蛋白質のOprRであることを明らかとした．またその耐性化株でOprRのノックアウト株を作成したところ，第四アンモニウム塩系消毒剤に対する感受性が野生株と同程度まで上昇することから，OprRがこの消毒薬耐性に重要な役割を果たしていることを示した． (Keyword) Adaptation, Physiological / Amino Acid Sequence / Bacterial Outer Membrane Proteins / Drug Resistance, Bacterial / Gene Deletion / Genes, Bacterial / Molecular Sequence Data / Mutagenesis / Pseudomonas aeruginosa / Quaternary Ammonium Compounds (Tokushima University Institutional Repository) ● Metadata: 117786 (Link to Publication Site) ● Publication site (DOI): 10.1128/AAC.47.7.2093-2099.2003 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12821452 ● Search Scopus @ Elsevier (PMID): 12821452 ● Search Scopus @ Elsevier (DOI): 10.1128/AAC.47.7.2093-2099.2003 (Tokushima University Institutional Repository: 117786, DOI: 10.1128/AAC.47.7.2093-2099.2003, PubMed: 12821452)
79.	Kazuto Ohkura, Akiko Sukeno, Keiko Yamamoto, Hideaki Nagamune, Takuya Maeda and Hiroki Kourai : Analysis of Structural Features of Bis-Quaternary Ammonium Antimicrobial Agents 4,4'-(α,ω-Polymethylenedithio)bis(1-alkylpyridinium Iodide)s Using Computational Simulation, Bioorganic & Medicinal Chemistry, Vol.11, No.23, 5035-5043, 2003. (Summary) 2個のピリジン環を持ち，その2環を結ぶブリッジ構造が異なる4タイプのビス型第四アンモニウム塩系消毒剤について，その分子構造とNB1RGB細胞に対する細胞毒性の構造活性相関を配座探索と分子軌道計算により検討した結果，他の3タイプの消毒剤はエネルギー極小状態がただ一つであるのに対し，ピリジンの四級窒素にオクチル基を持つチオエーテルブリッジタイプ(チオエーテル間のメチレン鎖数が4(4DTBP-4,8)と6(4DTBP-6,8))の場合だけエネルギー極小状態が2つ存在した．この結果から，4DTBP-4,8と4DTBP-6,8が高い効力と安全性を示すのは，分子の柔軟性と疎水性度が上手くバランスしているためであることを示した． (Keyword) Anti-Infective Agents / Computer Simulation / Microbial Sensitivity Tests / Molecular Structure / Niacinamide / Pyridinium Compounds / Quaternary Ammonium Compounds (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bmc.2003.08.027 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14604666 ● Search Scopus @ Elsevier (PMID): 14604666 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bmc.2003.08.027 (DOI: 10.1016/j.bmc.2003.08.027, PubMed: 14604666)
80.	Daxin Zhang, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Bactericidal Action of a Bis-quaternary Ammonium Compound against Vegetative Cells and Spores of Bacillus subtilis, Biocontrol Science, Vol.8, No.3, 101-110, 2003. (Summary) 論文題目:Bacillus subtilisの栄養細胞および芽胞に対するビス型第四アンモニウム塩の殺菌活性ビス型第四アンモニウム塩は4,4'-(1,6-ヘキサメチレンジチオ)ビス(1-オクチルピリジニウムアイオダイド)を用い，枯草菌の栄養細胞と芽胞に対する殺菌活性の測定，芽胞発芽阻害作用の検討，殺菌速度および殺菌活性化エネルギーの測定，pH依存性などについて詳細に検討した．その結果，本薬剤は芽胞に対して発芽阻害作用と発芽後の増殖も阻害することが明らかとなった．また，殺菌活性化エネルギーは非常に低く細胞への吸着段階が殺菌の律速段階であることがわかった． (Keyword) Bis-quaternary ammonium compouno / Bacillus subtilis / Bactericidal activity / Rate constant / Activation energy / Germination (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.8.101 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001204463823232 ● Summary page in Scopus @ Elsevier: 2-s2.0-0141891898 (DOI: 10.4265/bio.8.101, CiNii: 1390001204463823232, Elsevier: Scopus)
81.	Atsushi Tabata, Daxin Zhang, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Microbial Contamination in Home Laundry Operations in Japan, Biocontrol Science, Vol.8, No.1, 9-18, 2003. (Summary) 論文題目:日本における家庭洗濯の微生物汚染．家庭洗濯中の衛生レベルは生活様式の変化と共に変遷してきた．諸外国では洗濯中に微生物汚染や感染が報告されている．そこで日本における洗濯中の汚染について様々な着用後の繊維製品に付着している微生物種の同定，洗濯物間の微生物転移による感染の危険性の実態について明らかにした．さらに除菌洗剤と非除菌洗剤を用いて検討し，除菌洗剤の汚染や感染防止効果が高いことを明らかにした． (Keyword) Home laundry / Japan / Microbial contamination / Microbial transmission / Bath water (Tokushima University Institutional Repository) ● Metadata: 117799 (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.8.9 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001204464267648 ● Search Scopus @ Elsevier (DOI): 10.4265/bio.8.9 (Tokushima University Institutional Repository: 117799, DOI: 10.4265/bio.8.9, CiNii: 1390001204464267648)
82.	Atsushi Tabata, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : The Properties of Bacterial Adaptation to Mono-and Bis-Quaternary Ammonium Compounds, Biocontrol Science, Vol.8, No.1, 37-42, 2003. (Summary) 論文題目:モノ型およびビス型第四アンモニウム塩に対する細菌の適応の性質．モノ型(デシルピリジニウム塩)およびビス型第四アンモニウム塩(架橋構造がチオール，エステル，カルバモイルおよびアミド)に対する細菌の適応について検討した．最小発育阻止濃度(MIC)測定法により検討した結果，モノ型ではMIC値が上昇し，抵抗性化が見受けられたが，ビス型第四アンモニウム塩では全く耐性化が見られなかった．抵抗性菌の表面疎水性を解析した結果，モノ型の抵抗性化は細胞表面の疎水性が低下したためであるとした． (Keyword) Quaternary ammonium compound / Bis-QAC / Bacterial adaptation / Resistance to disinfectant / Cell surface hydrophobicity (Tokushima University Institutional Repository) ● Metadata: 117798 (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.8.37 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282679441002368 ● Search Scopus @ Elsevier (DOI): 10.4265/bio.8.37 (Tokushima University Institutional Repository: 117798, DOI: 10.4265/bio.8.37, CiNii: 1390282679441002368)
83.	Atsushi Tabata, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Characterization of Pseudomonas aeruginosa Resistant to a Quaternary Ammonium Compound, Biocontrol Science, Vol.7, No.3, 147-153, 2002. (Summary) 論文題目:第四アンモニウム塩化合物に対して薬剤耐性を示す緑膿菌の特徴 N-ドデシルピリジニウムアイオダイドに対する薬剤耐性化を最小濃度測定法である培地希釈法を用いて行うことに成功した．その結果，感受性菌よりも8倍高い耐性化細菌を得ることができた．電子顕微鏡で観察した結果，耐性化細胞は感受性細胞には見られないバイオフィルム様の凝集が観察された．さらに，耐性化した細胞の表面疎水性について測定した結果，感受性細胞よりも親水性化していることがわかった． (Keyword) Quaternary ammonium compound / Pseudomonas aeruginosa / Disinfectant resistance / Cell surface hydrophobicity / Biofilm (Tokushima University Institutional Repository) ● Metadata: 117800 (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.7.147 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001204463971328 ● Search Scopus @ Elsevier (DOI): 10.4265/bio.7.147 (Tokushima University Institutional Repository: 117800, DOI: 10.4265/bio.7.147, CiNii: 1390001204463971328)
84.	Akihiro Shirai, Takuya Maeda, Ichiro Hara, Hideaki Nagamune and Hiroki Kourai : Synthesis and antimicrobial characteristics of 4,4',4''-(1,2,3-propylene trithio)tris(1-alkylpyridinium iodide)s, Biocontrol Science, Vol.7, No.2, 55-61, 2002. (Summary) トリス型四級アンモニウム化合物 (tris-QAC) 4,4',4''-(1,2,3-propylene-trithio)tris (1-alkylpyridinium iodide)s (4TP-n: アルキル基の炭素数 n = 4,6,8,10,12,14)を合成し，抗菌特性を検討した．4TP-nは，3つのN-alkyl-4-thiopyridinium iodideをプロパンの1,2,3位で結合したトリス型構造を有する．4TP-nの抗菌特性を，ビス型四級アンモニウム化合物 (bis-QAC) 4,4'-(1,6-hexamethylenedithio)bis(1-alkylpyridinium iodide)s (4DTBP-6,n)，モノ型四級アンモニウム化合物 (mono-QAC) N-alkylpyridinium iodide (P-n)，そして商業的な抗菌剤，防黴剤と比較した．4TP-nの静菌，殺菌活性は，4DTBP-6,nと同様に高く，またアルキル鎖長の影響をほとんど受けなかった．4TP-nは，広くかつ強い抗菌スペクトルを有していた．4TP-nの殺菌活性は，pH，温度の影響を受け，その影響を全く受けない4DTBP-6,8とは異なっていた．
85.	Takatsugu Goto, Hideaki Nagamune, Aiko Miyazaki, Yoshiaki Kawamura, Ooki Ohnishi, Kanako Hattori, Kazuto Ohkura, Kazuaki Miyamoto, Shigeru Akimoto, Takayuki Ezaki, Katsuhiko Hirota, Yoichiro Miyake, Takuya Maeda and Hiroki Kourai : Rapid identification of Streptococcus intermedius by PCR with the ily gene as a species marker gene, Journal of Medical Microbiology, Vol.51, No.2, 178-186, 2002. (Summary) ヒト特異的細胞溶解毒素インターメディリシン(ILY)遺伝子はアンギノーサス群連鎖球菌ではS. intermedius(SI)のみに特異的に存在したことから，ily遺伝子が本菌種にだけ分布する可能性が考えられた．そこでPCRやサザンドットハイブリダイゼーション法を用いて，連鎖球菌属や近縁菌種におけるily遺伝子の分布を調べ，ily遺伝子がSI特異的な菌種マーカーになり得ることを確認し，それを指標として本菌種による感染症診断に応用可能なPCR検出システムを構築した． (Keyword) MILLERI GROUP / ANGINOSUS GROUP / LIVER-ABSCESS / CONSTELLATUS / STRAINS / SYSTEM / HYBRIDIZATION (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11863269 ● Search Scopus @ Elsevier (PMID): 11863269 (PubMed: 11863269)
86.	Marion G. Macey, Robert A. Whiley, L. Miller and Hideaki Nagamune : Effect on Polymorphonuclear Cell Function of a Human-Specific Cytotoxin, Intermedilysin, Expressed by Streptococcus intermedius, Infection and Immunity, Vol.69, No.10, 6102-6109, 2001. (Summary) Streptococcus intermedius is a member of the normal flora of the mouth but is also an opportunistic pathogen associated with purulent infections at oral and nonoral sites. Intermedilysin (ILY) has been shown to be a cytolysin capable of generating pores in the cell membrane of erythrocytes demonstrable by electron microscopy. This effect has been shown to be specific for human cells. Since polymorphonuclear cells (PMNs) are the main cell involved in innate immunity we investigated the effect of purified intermedilysin from Streptococcus intermedius on PMN function. Active ILY at a concentration of 40 ng/microl caused a significant decrease in the number of intact PMNs after 60 min. The active cytolysin, when compared with heat-inactivated ILY, did not appear to be chemotactic for the PMNs but did cause an increase in intracellular calcium, with increased cell surface CD11b expression, metabolic burst, and phagocytosis of Staphylococcus aureus. These findings may have implications for the role of ILY in deep-seated abscesses. (Keyword) Bacterial Proteins / Bacterial Toxins / Bacteriocins / Calcium / Cell Fusion / Cell Membrane Permeability / Cell Size / Chemotaxis, Leukocyte / Cytotoxins / Flow Cytometry / Hemolysis / Humans / Intracellular Fluid / Leukocyte Count / Macrophage-1 Antigen / Neutrophils / Phagocytosis / Staphylococcus aureus / Streptococcus (Link to Publication Site) ● Publication site (DOI): 10.1128/IAI.69.10.6102-6109.2001 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11553548 ● Search Scopus @ Elsevier (PMID): 11553548 ● Search Scopus @ Elsevier (DOI): 10.1128/IAI.69.10.6102-6109.2001 (DOI: 10.1128/IAI.69.10.6102-6109.2001, PubMed: 11553548)
87.	Munehiro Yoshida, Takuya Maeda, Kiyo Okazaki, Hideaki Nagamune, Kensei Kunikata, Hiroshi Tsuchiya, Tetsuto Namba and Hiroki Kourai : Synthesis of 4,4'-(Tetramethylenedicarbonyldiamino)bis(1-decylpyridinium bromide) and Its Antimicrobial and Deodorant Characteristics, Biocontrol Science, Vol.6, No.2, 75-80, 2001. (Summary) 論文題目:4,4'-(テトラメチレンジカルボニルジアミノ)ビス(1-デシルピリジニウムブロマイド)．本論文において4,4'-(テトラメチレンジカルボニルジアミノ)ビス(1-デシルピリジニウムブロマイド)の新規合成方法の確立を行い，さらに一連の合成薬剤の抗菌特性を塩化ベンザルコニウム，チアベンダゾール，ベンツイミダゾールおよびセチルピリジニウムと比較検討した．その結果，細菌類に対して塩化ベンザルコニウムやセチルピリジニウムよりも非常に高い静菌活性を認めた．また，黴にたいしても現行のチアベンダゾールやベンツイミダゾールよりも高い活性を示した．さらに消臭効果やプラスチックに対する抗菌効果などの応用試験を行った． (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.6.75 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0034791731 (DOI: 10.4265/bio.6.75, Elsevier: Scopus)
88.	Munehiro Yoshida, Takuya Maeda, Kiyo Okazaki, Hideaki Nagamune, Kensei Kunikata, Hiroshi Tsuchiya, Tetsuto Namba and Hiroki Kourai : Synthesis and Antimicrobial Characteristics of N,N'-Hexamethylenebis(4-carbamoyl-1-decylpyridinium bromide), Biocontrol Science, Vol.5, No.2, 65-71, 2000. (Summary) 論文題目:N,N'-ヘキサメチレンビス(4-カルボニル-1-デシルピリジニウムブロマイド)の合成と抗菌特性．ビス型の第四アンモニウム塩であるN,N'-ヘキサメチレンビス(4-カルボニル-1-デシルピリジニウムブロマイドの合成方法を確立し，抗菌特性についてモノ型のアルキルピリジニウムを用いて比較検討した．その結果，非常に優れた殺菌活性を示し，かつ，高い抗黴活性も示した．pH依存性や温度依存性についても検討し，モノ型よりも優れた特性を認めた．静菌活性，殺菌活性および抗黴活性は，塩化ベンザルコニウム，セチルピリジニウムおよびチアベンダゾールよりも高い活性を示した．
89.	Hideaki Nagamune, Takuya Maeda, Kazuto Ohkura, Keiko Yamamoto and Hiroki Kourai : Evaluation of the cytotoxic effects of bis-quaternary ammonium antimicrobial reagents on human cells, Toxicology In Vitro, Vol.14, No.2, 139-147, 2000. (Summary) The cytotoxic effects of newly synthesized bis-quaternary ammonium compounds (bis-QACs) and benzalkonium chloride were investigated on skin and blood cells, namely normal human epidermal keratinocytes of neonatal foreskin, a normal human skin fibroblast cell line NB1RGB, erythrocytes and a lymphoma cell line JM. The bis-QACs tested were 4, 4'-(1,6-hexamethylenedithio)bis(1-octylpyridinium iodide) (4DTBP-6, 8), N,N'-tetramethylenebis(1-dodecyl-4-carbamoylpyridinium iodide) (4BCAP-4,12), N,N'-hexamethylenebis(1-decyl-4-carbamoylpyridinium iodide) (4BCAP-6,10), 4,4'-(1,4-tetramethylenedicarbonyldiamine) bis (1-decylpyridinium iodide) (4DCABP-4,10), 4,4'-(1, 4-tetramethylenedicarbonyldiamine) bis (1-dodecylpyridinium iodide) (4DCABP-4,12), 4,4'-(1, 4-phenyldicarbonyldiamine)bis(1-dodecylpyridinium iodide) (4DCABP-P, 12), and 4,4'-(1, 6-hexamethylenedioxydicarbonyl)bis(1-dodecylpyridinium iodide) (4DOCBP-6,12). All bis-QACs consisted of two identical alkylpyridinium rings and bridge structure linking rings to each other have a methylene bridge but only 4DCABP-P,12 has a phenyl ring as a bridge. Most of the LD(50) values in acute cytotoxic assays of these bis-QACs tended to be lower than those of benzalkonium chloride. However, the comparison of the antibacterial activity against cytotoxic effects on several human cells revealed that bis-QACs, especially 4DTBP-6,8, have wide concentration ranges showing sufficient antibacterial activity and lower cytotoxic effect on human cells (except for 4DOCBP-6,12), although benzalkonium chloride caused significant human cell damage at the concentrations necessary for antibacterial activity. Moreover, judging from the LD(50) value of 4DTBP-6,8 [67 microM, a 6.7-fold higher concentration than the upper value of MIC] obtained in an artificial human skin model TESTSKIN, 4DTBP-6,8 is thought to be a more effective and safer antiseptic reagent for application to the skin surface than benzalkonium chloride. Taken together, bis-QACs are expected to be a promising new generation of antimicrobial QACs. (Keyword) Anti-Bacterial Agents / Bacteria / Benzalkonium Compounds / Cell Line / Cell Survival / Erythrocytes / Fibroblasts / Humans / Indicators and Reagents / Keratinocytes / Lymphocytes / Lymphoma, T-Cell / Microbial Sensitivity Tests / Preservatives, Pharmaceutical / Quaternary Ammonium Compounds (Link to Publication Site) ● Publication site (DOI): 10.1016/S0887-2333(00)00003-5 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10793292 ● CiNii @ National Institute of Informatics (CRID): 1571135651719773952 ● Summary page in Scopus @ Elsevier: 2-s2.0-0033997301 (DOI: 10.1016/S0887-2333(00)00003-5, PubMed: 10793292, CiNii: 1571135651719773952, Elsevier: Scopus)
90.	Hideaki Nagamune, Robert A. Whiley, Takatsugu Goto, Yasuko Inai, Takuya Maeda, Jeremy M. Hardie and Hiroki Kourai : Distribution of the intermedilysin gene among the anginosus group streptococci and correlation between intermedilysin production and deep-seated infection with Streptococcus intermedius, Journal of Clinical Microbiology, Vol.38, No.1, 220-226, 2000. (Summary) The distribution of intermedilysin, a human-specific cytolysin, among the anginosus group streptococci and the correlation of toxin production and infection by Streptococcus intermedius were investigated. PCR and Southern hybridization specific for the intermedilysin gene revealed that the toxin gene exists only in S. intermedius and no homologue to the toxin gene is distributed in S. anginosus and S. constellatus. Thus, the intermedilysin gene is useful as a marker gene of S. intermedius. Moreover, a human-specific hemolysis assay and Western blotting with intermedilysin-specific antibodies clearly demonstrated that the intermedilysin production level in isolates from deep-seated infections, such as brain and liver abscesses, is higher (6.2- to 10.2-fold, respectively) than in strains from normal habitats, such as dental plaque, or from peripheral infection sites. However, other candidate virulence factors of S. intermedius, such as chondroitin sulfate depolymerase, hyaluronidase, and sialidase activities, did not show such a clear correlation between enzymatic activity and isolation sites or disease severity. From these results, intermedilysin is likely to be the pathogenic or triggering factor of significance in inducing deep-seated infections with S. intermedius. (Keyword) Gene Expression / Genes, Bacterial / Glycoside Hydrolases / Humans / Mouth / Polymerase Chain Reaction / Streptococcal Infections / Streptococcus (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10618091 ● Summary page in Scopus @ Elsevier: 2-s2.0-0033628592 (PubMed: 10618091, Elsevier: Scopus)
91.	Katsuhiko Hirota, Hiromi Murata, Hiromichi Yumoto, Kazuto Ohkura, Hideaki Nagamune and Yoichiro Miyake : Three-dimensional structural analysis of histone-like protein from streptococci, Bacterial Adherence研究, Vol.14, 6-8, 2000.
92.	Takuya Maeda, Munehiro Yoshida, Yuki Manabe, Kiyo Okazaki, Hideaki Nagamune and Hiroki Kourai : Synthesis and Antimicrobial Characteristics of 5,5'-[2,2'- (Tetramethylenedicarbonyldioxy) diethyl]bis(3-alkyl-4-methylthiazolium iodide)s, Biocontrol Science, Vol.4, No.2, 75-81, 1999. (Summary) 論文題目:5,5'-[2,2'-(テトラメチレンジカルボニルジオキシ)ジエチル]ビス(3-アルキル-4-メチルチアゾリウムアイオダイド)の合成と抗菌特性．本論文においてビス型のチアゾリウム系抗菌剤および対応するモノ型チアゾリウムを合成し，抗菌特性，静菌スペクトル，静黴スペクトル，pHおよび温度特性などを検討した結果，優れた抗菌特性であることを明らかにした．さらに分子疎水性パラメーターを用い殺菌活性と静菌活性を解析した結果，殺菌活性が分子疎水性に依存しない優れた性質を有することを明らかにした．
93.	Takuya Maeda, Yuki Manabe, Masashi Yamamoto, Munehiro Yoshida, Kiyo Okazaki, Hideaki Nagamune and Hiroki Kourai : Synthesis and Antimicrobial Characteristics of Novel Biocides, 4,4'-(1,6-Hexamethylenedioxy dicarbonyl)bis(1-alkylpyridinium iodide)s, Chemical & Pharmaceutical Bulletin, Vol.47, No.7, 1020-1023, 1999. (Summary) 非常に高い殺菌活性を有するビス-エステル型の4,4'-(1,6-ヘキサメチレンジオキシジカルボニル)ビス(1-アルキルオイリジニウムアイオダイド)類を6種合成し，その殺菌活性，殺菌特性およびヒト細胞毒性について検討した．これらの薬剤は細菌のみならずカビに対しても非常に高い殺菌活性を示し，現状の殺菌剤，抗カビ剤に比較し数十倍の活性であった．さらに細胞毒性は現状の殺菌剤の十分の一であった．
94.	Kiyo Okazaki, Munehiro Yoshida, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Synthesis and Bactericidal Characteristics of N-Alkyl-2-benz ylaminopyridinium Iodides, Biocontrol Science, Vol.4, No.1, 17-22, 1999. (Summary) 2位にベンジルアミノ基を導入したN-アルキル-2-ベンジルアミノピリジニウムアイオダイドを合成し，殺菌活性に及ぼすアンモニウムヘッドのかさ高さの効果および殺菌特性について検討した．ベンジルアミノ基の導入により殺菌活性が上昇したが，分子疎水性の効果はN-アルキルピリジニウムアイオダイドと同じ傾向を示したがpHの影響を受けなくなり，細胞表面疎水性解析から，かさ高さの効果を考察した．
95.	Takuya Maeda, Kiyo Okazaki, Hideaki Nagamune, Yuki Manabe and Hiroki Kourai : Bactericidal Action of 4,4'-(α,ω-Polymethylenedithio) bis(1-alkylpyridinium iodide)s, Biological & Pharmaceutical Bulletin, Vol.21, No.10, 1057-1061, 1998. (Summary) 強力な殺菌活性を示す4,4'-(α ω-ポリメチレンジチオ)ビス(アルキルピリジニウムアイオダイド)の殺菌機構をlog HI， RM，log CVC，SEM観察から解析した．殺菌活性はRMの影響を受けないが静菌活性は強く影響を受け，log HIを用いて解析した結果，培地成分と薬剤の会合であることが判明し，薬剤分子を親水化することによりスペクトルの拡大と共存物質の影響を排除できることを示した．
96.	Kiyo Okazaki, Takuya Maeda, Hideaki Nagamune, Yuki Manabe and Hiroki Kourai : Synthesis and Antimicrobial Characteristics of 4,4'-(α,ω-Polymethylenedithio) bis(1-alkylpyridinium iodide)s, Chemical & Pharmaceutical Bulletin, Vol.45, No.12, 1970-1974, 1997. (Summary) アルキル鎖長とメチレン鎖長の異なるビス型第四アンモニウム塩，4,4'-α，ω-ポリメチレンジチオ)ビス(アルキルピリジニウムアイオダイド)を30種合成し，殺菌活性，静菌活性および静菌スペクトルについてアルキルピリジニウムアイオダイドを比較薬剤として解析した．ビス型はメチレン及びアルキル鎖長の長短に関係なく一定の高い殺菌活性を示し，温度やpHの影響をも受けない特異的殺菌機構であることを示した．
97.	Hideaki Nagamune, Hiroki Kourai, Ayako Katsuura, Yasuki Taoka, Kenzo Fushitani, Robert A. Whiley, Kikuji Yamashita, Akihiko Tsuji, Yoshiko Matsuda, Hiroki Kourai and Seiichiro Kitamura : Intermedilysin A cytolytic toxin specific for human cells of a Streptococcus intermedius isolated from human liver abscess in "Streptococci and The Host", Advances in Experimental Medicine and Biology, Vol.418, 773-775, 1997. (Summary) S. intermedius肝膿瘍分離株から単離されたヒト細胞特異的な細胞溶解毒素インターメディリシン(ILY)の性質を検討した．これまで知られていたチオール基修飾試薬への非感受性やコレステロールへの低感受性に加え，電子顕微鏡観察からILYは直径30-50nmの膜孔を形成し細胞を破壊することが示された．またILYの部分遺伝子構造解析の結果からILYはチオール活性化毒素群と同じ分子祖先から進化したものであることが示唆された．
98.	Tetsuya Akamatsu, Shigeo Daikoku, Hideaki Nagamune, Shigeru Yoshida, Kenji Mori, Akihiko Tsuji and Yoshiko Matsuda : Developmental expression of a novel Kexin family protease, PACE4E, in the rat olfactory system., Histochemistry and Cell Biology, Vol.108, No.2, 95-103, 1997. (Summary) 本研究では新規Kexin family proteaseであるPACE4Eがラット脳発生過程において時期·空間特異的に発現することを明らかにし, 特に嗅球においては僧帽細胞に極めて特異的に発現し, 僧帽細胞の分化成熟と密接に関わることを見い出した. (Keyword) Processing protease / Olfactory system / Mitral cell
99.	Kiyo Okazaki, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Antibacterial Characteristics of N-Alkyl-2-alkylthiopyridinium and N-Alkyl-4-alkylthiopyridinium Salts, Japanese Journal of Toxicology and Environmental Health, Vol.43, No.5, 251-260, 1997. (Summary) N-アルキル-2-アルキルチオピリジニウムとN-アルキル-4-アルキルチオピリジニウム塩を14種合成し，2-アルキルチオ基と4-アルキルチオ基の殺菌活性に及ぼす効果について細菌細胞表面疎水性(log HI)， RM，log CVC，SEM観察，温度効果を用いて解析した． 2-アルキルチオ基と4-アルキルチオ基の導入は殺菌活性を高くし，かつ，log Pの影響が低下することを明らかにした． (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0031436095 (Elsevier: Scopus)
100.	Kenji Mori, Sachiko Kii, Akihiko Tsuji, Masami Nagahama, Akiyoshi Imamaki, Keiko Hayashi, Tetsuya Akamatsu, Hideaki Nagamune and Yoshiko Matsuda : A Novel Human PACE4 Isoform, PACE4E Is an Active Processing Protease Containing a Hydrophobic Cluster at the Carboxy Terminus, The Journal of Biochemistry, Vol.121, No.5, 941-948, 1997. (Summary) ズブチリシン様プロプロテインコンベルターゼファミリーに属するPACE4の新規アイソフォームPACE4E-I, PACE4E-IIをヒト脳cDNAライブラリーよりクローニングした．PACE4Eは，PACE4Aと比べ，C末に75残基からなる，疎水性アミノ酸のクラスタを含むユニークな配列を持っていた．この部分を欠失したPACE4Eは分泌速度が上昇した．共発現実験によって，PACE4E-1, E-IIは，PACE4Aと同様にプロセシング活性を持っていることが証明された．
101.	Kiyo Okazaki, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Synthesis and antimicrobial characteristics of N-alkyl-4-allythiopyridinium bromides, Biocontrol Science, Vol.2, No.1, 39-42, 1997. (Summary) ピリジニウム骨格のアリールチオ基を有するN-アルキル-4-アリールチオピリジニウムアイオダイド類を合成し，N-アルキル-4-ブテニルピリジニウムアイオダイドならびにN-アルキルピリジニウムアイオダイドを比較薬剤として4-位のチオエーテル結合に着目して殺菌活性を解析した．チオエーテルを挿入した薬剤はブテニルピリジニウムよりも高い殺菌活性を示し，ヘッドのかさ高さの効果であることを明らかにした．
102.	Hideaki Nagamune, Kazuaki Muramatsu, Masakatsu Higashine, Tetsuya Akamatsu, Yasuhiro Tamai, Yasuo Yonetomi, Akihiko Tsuji, Keisuke Izumi, Takemasa Sakaguchi, Tetsuya Yoshida and Yoshiko Matsuda : Cyclic AMP-inducible procalcitonin processing in thyroidal parafollicular cells is regulated by the kexin family protease, PC1, Biomedical and Health Research, Vol.15, 22-33, 1997. (Summary) 甲状腺におけるカルシトニンのプロセシングに関与するズブチリシン様プロプロテインコンベルターゼを同定するため，特異抗体による免疫染色とモデル細胞TT細胞におけるcAMPの影響について調べた．カルシトニン産生細胞で発現していたのは，主にPC1とPC2であったが，カルシトニンの産生を刺激するcAMPによって発現が増加したのは，PC1であった．これらの結果は，PC1がプロカルシトニンの活性化酵素であることを示している．
103.	Hideaki Nagamune, Chikako Ohnishi, Ayako Katsuura, Kenzo Fushitani, Robert A. Whiley, Akihiko Tsuji and Yoshiko Matsuda : Intermedilysin, a novel cytotoxin specific for human cells, secreted by Streptococcus intermedius UNS46 isolated from a human liver abscess, Infection and Immunity, Vol.64, No.8, 3093-3100, 1996. (Summary) S. intermediusのヒト肝膿瘍分離株がヒト細胞特異的な細胞溶解毒素インターメディリシン(ILY)を分泌することを発見した． ILYは約54KDaの蛋白で，その部分一次構造はニューモリシンと高い相同性を示した．しかしSH修飾試薬により失活せず，コレステロール対しても感受性が低かった．またトリプシン処理したヒト赤血球はILYに対して抵抗性を示すようになることから，ILY作用には宿主細胞膜の蛋白質が関与している事が示された．
104.	Kiyo Okazaki, Yuki Manabe, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Quantitative structure-activity relationship of antibacterial dodecylpyridinium iodide derivatives, Biocontrol Science, Vol.1, No.1, 51-59, 1996. (Summary) 抗菌性ヨウ化ドデシルピリジニウム塩の各種誘導体の定量的構造活性相関，ドデシルピリジニウム塩のピリジン環に各種置換基を導入した薬剤を12種合成し，前報の17種を会わせて29種を用いて抗菌活性を定量的に解析した．抗菌活性パラメーターとして殺菌活性(MBC)および臨界微生物細胞破壊濃度(CVC)，物理化学パラメーターとして酸解離定数(pKa)，アンモニウム窒素隣接メチレンプロトンのケミカルシフト(δ)，分子疎水性を用いて検討した結果，抗菌活性はアルキル鎖長をドデシル基に限定すると分子創始性の依存度は少なくなり，酸解離定数，ケミカルシフト，電子密度に依存することを見出した．
105.	Takuya Maeda, Seiko Goto, Yuki Manabe, Kiyo Okazaki, Hideaki Nagamune and Hiroki Kourai : Bactericidal action of N-alkylcyanopyridinium bromides against Escherichia coli K12 W3110, Biocontrol Science, Vol.1, No.1, 41-49, 1996. (Summary) 臭化N-アルキルシアノピリジニウムのEscherichia coli K12 W3110に対する殺菌作用，本論文はオルト，メタ，パラ位にシアノ基を導入した臭化N-アルキルシアノピリジニウム(アルキル基:オクチル∼ステアリル)を18種類合成し，大腸菌に対する殺菌特性ならびに殺菌メカニズムに関する検討を行った結果を纏めたものである．2位にシアノ基を挿入した場合には殺菌活性と静菌活性が著しく上昇し，アルキル鎖長がドデシル以上の長さでは高い一定の活性を示した．殺菌活性が酸解離定数(pKa)に依存した．薬剤作用による細胞懸濁液の濁度が低下する溶菌現象について電子顕微鏡写真やタンパク質合成阻害剤の抗生物質を用いて解析した結果，物理的な細胞破壊作用が主たる作用であることを明らかにした．
106.	Akihiko Tsuji, Keisuke Edazawa, Koji Sakiyama, Kaya Nagata, Yuka Sasaki, Hideaki Nagamune and Yoshiko Matsuda : Purification and characterization of a novel serine proteinase from the microsomal fraction of bovine pancreas., The Journal of Biochemistry, Vol.119, No.1, 100-105, 1996. (Summary) 牛膵臓膜画分より，CHAPSによる可溶化，ゲルろ過，トリプシンインヒビターセファロース，アルギニンーセファロースによって，新規のトリプシン様セリンプロテアーゼを単一に精製した．この酵素は分子量29500で，グルカゴンを用いて切断特異性を調べると，塩基性アミノ酸とロイシンのC末側を切断した．N末のアミノ酸配列は，エラスターゼIV, II, IIIに類似していた．
107.	Akihiko Tsuji, Hideaki Nagamune, Tetsuya Akamatsu, Chiemi Hine, Kazuaki Muramatsu, Kenji Mori, Yasuhiro Tamai, Kayoko Yonemoto and Yoshiko Matsuda : Novel members of mammalian kexin family proteases, PACE4C, PACE4D, PC7A and PC7B., Intracellular Protein Catabolism, Vol.389, 63-71, 1996.
108.	Hideaki Nagamune, Kazuaki Muramatsu, Tetsuya Akamatsu, Yasuhiro Tamai, Keisuke Izumi, Akihiko Tsuji and Yoshiko Matsuda : Distribution of the kexin family proteases in pancreatic islets., --- PACE4C is specifically expressed in B cells of pancreatic islets ---, Endocrinology, Vol.136, No.1, 357-360, 1995. (Summary) Kexin Family Proteasesはペプチドホルモン等のプロセシング酵素として同定され, 血糖調節ホルモンのインスリン等のプロセシングにも関与することが示唆されていたが, 本研究では我々が新規にクローニングしたPACE4CをはじめとするKexin Family Proteasesとインスリン等との膵島細胞での局在, 共存関係を初めて明らかにし, その成果は雑誌表紙にも掲載された. (Keyword) Precessing protease / Pancreatic islet / Insulin / Glucagon
109.	Akihiko Tsuji, Chiemi Hine, Kenji Mori, Yasuhiro Tamai, Kazuya Higashine, Hideaki Nagamune and Yoshiko Matsuda : A Novel Member, PC7, of The Mammalian Kexin-Like Protease Family, --- Homology to PACE4A, Its Brainspecific Expression and Identification of Isoforms ---, Biochemical and Biophysical Research Communications, Vol.202, No.3, 1452-1459, 1994. (Summary) 哺乳類には7種類のKexin様プロプロテインコンベルターゼが存在する．哺乳類Kexin様プロテアーゼ間で保存された触媒領域のプライマーを用い，ラット脳下垂体からcDNA断片をPCR増幅し，これをプローブにしてラット脳下垂体cDNAライブラリーからPC7をクローニングした．PC7(PC7AとPC7B)はRXKRの活性化サイトを持ち，触媒，HomoB及びシステインリッチ領域を持っており，PACE4Aと非常に高いホモロジーを示した．PC7は脳で強く発現し，肝，腎，心，脾では検出されなかった．
110.	Akihiko Tsuji, Kenji Mori, Chiemi Hine, Yasuhiro Tamai, Hideaki Nagamune and Yoshiko Matsuda : The tissue distribution of mRNAs for the PACE4 isoforms, kexin-like processing protease: PACE4C and PACE4D mRNAs are major transcripts among PACE4 isoforms, Biochemical and Biophysical Research Communications, Vol.202, No.3, 1215-1221, 1994. (Summary) 新たに見いだされたPACE4アイソフォーム(PACE4CとPACE4D)の組織分布をRT-PCR法を用いて解析した．両アイソフォームのmRNAともに，PACE4Aと同様に多くの組織で見いだされたが，それぞれの発現パターンは異なっていた．また，PACE4C及びPACE4DのmRNAはPACE4Aのそれより遙かに多く，PACE4 mRNAの主要な部分はPACE4CとPACE4DのmRNAである事が示唆された．
111.	Akihiko Tsuji, Ryoichi Oda, Kohji Sakiyama, Hideaki Nagamune, Kouji Itou, Ryoichi Kase, Hitoshi Sakuraba, Yoshiyuki Suzuki and Yoshiko Matsuda : Lysosomal enzyme replacement using α2-macroglobulin as a transport vehicle, The Journal of Biochemistry, Vol.115, No.5, 937-944, 1994. (Summary) α2-マクログロブリン(α2M)を運搬担体に用いたリソソーム酵素欠損性遺伝病の酵素補充療法システムを開発した． α2Mはレセプターを介してリソソームに取り込まれることから，豚肝臓から精製した α-グルコシダーゼ或いはα-ガラクトシダーゼにα2Mを運搬担体として二価架橋試薬で連結し，リソソーム指向性の酵素補充療法剤を構築した．これを用いてin vitro実験を行い，ポンペ病及びファブリー病細胞の細胞病態が改善されることを証明した．
112.	Akihiko Tsuji, Kazuya Higashine, Chiemi Hine, Kenji Mori, Yasuhiro Tamai, Hideaki Nagamune and Yoshiko Matsuda : Identification of Novel cDNAs Encording Human Kexin-like Protease, PACE4 Isoforms, Biochemical and Biophysical Research Communications, Vol.200, No.2, 943-950, 1994. (Summary) ヒト胎盤cDNAライブラリーより，新規のPACE4アイソフォーム，PACE4CとPACE4Dをクローニングし，塩基配列を決定した．PACE4Cは，PACE4AのC末に存在するシステインリッチ領域を持たず，ホモBドメインのC末にC-特異的配列を持っていた．PACE4Dはシグナルペプチド，プロペプチドおよびシステインリッチ領域を欠失していた．
113.	Akihiko Tsuji, Tetsuya Akamatsu, Hideaki Nagamune and Yoshiko Matsuda : Identification of targeting proteinase for rat α1-macroglobulin in vivo., --- Mast-cell tryptase is a major component of the α1-macroglobulin-proteinase complex endocytosed into rat liver lysosomes. ---, The Biochemical Journal, Vol.298, No.1, 79-85, 1994. (Summary) 血清蛋白質であるα-マクログロブリン(αM)は種々の酵素に対するインヒビターとして機能すると考えられていたが, 生体内で標的となる酵素は不明であった. 本研究ではαMが酵素と複合体形成後, リソゾームに運ばれ分解されることに着目し, リソゾーム内の分解を抑制したラット肝臓リソゾームよりαM-酵素複合体を単離し, 肥満細胞トリプターゼが生体内でαMの標的酵素の1つであることを初めて明らかにした. (Keyword) α-macroglobulin / Mast-cell tryptase / Lysosome
114.	Hideaki Nagamune, Yoshihiro Fukushima, Junji Takada, Kenji Yoshida, Akira Unami, Takashi Shimooka and Hiroshi Terada : The lipophilic weak base (Z)-5-methyl-2-[2-(1-naphthyl)ethenyl]-4-piperidinopyridine (AU-1421) is a potent protonophore type cationic uncoupler of oxidative phosphorylation in mitochondria, Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol.1141, No.2-3, 231-237, 1993. (Summary) The lipophilic weak base AU-1421 acts as a simple protonophoric uncoupler of oxidative phosphorylation in rat liver mitochondria judging from the following observations. In the absence of any carrier lipophilic anions or P(i), AU-1421 stimulated the rate of state 4 respiration maximally about 7-fold at a concentration of 30 nmol/mg mitochondrial protein. At the same maximum effective concentration, it also inhibited ATP synthesis, released oligomycin-inhibited state 3 respiration, dissipated the proton motive force in the energized state, and activated latent H(+)-ATPase. AU-1421 also allowed proton conduction in both mitochondrial membranes and liposomes. These actions of AU-1421 resemble those of the typical anionic uncoupler SF6847. A marked difference between the two was, however, that ATPase activation by AU-1421 was not suppressed at higher concentrations of AU-1421, whereas ATPase activated by SF6847 was suppressed on increase of the SF6847 concentration. The finding that this simple protonophoric cation acts as an uncoupler at a micromolar concentration is significant, because all true (i.e., protonophore type) uncouplers known so far are anionic not cationic. Thus, AU-1421 is a unique uncoupler of the protonophore type. (Keyword) Adenosine Triphosphate / Animals / Electron Transport / Hydrolysis / Male / Mitochondria, Liver / Naphthalenes / Oxidative Phosphorylation / Oxygen / Protons / Pyridines / Rats / Rats, Wistar / Uncoupling Agents (Link to Publication Site) ● Publication site (DOI): 10.1016/0005-2728(93)90047-J (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 8382953 ● Search Scopus @ Elsevier (PMID): 8382953 ● Search Scopus @ Elsevier (DOI): 10.1016/0005-2728(93)90047-J (DOI: 10.1016/0005-2728(93)90047-J, PubMed: 8382953)
115.	Fusao Ota, Katsuhiko Hirota, Hideaki Nagamune and Komei Fukui : Enzyme immunoassay and monoclonal antibodies for immunological studies of the mutans group of streptococci, Dentistry in Japan, Vol.27, No.1, 25-28, 1990. (Summary) 以前開発した多糖体抗原固相化EIA法によって，ミュータンス群連鎖球菌のS. cricetusとS. downeiiに対する単クローン抗体の免疫特異性を検討したところ，得られた2種類の単クローン抗体の一方は血清型a，d及びhに共通の多糖抗原を，他方は血清型d，g及びhに共通の多糖抗原を認識するものであった．この研究において多糖体抗原固相化EIAシステムは単クローン抗体を用いた口腔連鎖球菌の同定や免疫学的研究に有用であることを実証した． (Keyword) Monoclonal Antibody / Oral Streptococcus / EIA / Polysaccharide / Immunoplate
116.	Osamu Urayama, Hideaki Nagamune, Makoto Nakao and Yukichi Hara : A monoclonal antibody against a native conformation of the porcine renal Na+/K+-ATPase -subunit protein, Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol.1040, No.2, 267-275, 1990. (Summary) A monoclonal antibody (mAb50c) against the native porcine renal Na+/K(+)-transporting adenosinetriphosphatase (EC 3.6.1.37, ATP phosphohydrolase) (Na+/K(+)-ATPase) was characterized. The antibody could be classified as a conformation-dependent antibody, since it did not bind to Na+/K(+)-ATPase denatured by detergent and its binding was affected by the normal conformational changes of the enzyme induced by ligands. The binding was the greatest in the presence of Na+, ATP or Mg2+ (E1 form), slightly less in the presence of K+ (E2K form) and the least when the enzyme was phosphorylated, especially in the actively hydrolyzing form in the presence of Na+, Mg2+ and ATP. The antibody inhibited both the Na+,K(+)-ATPase activity and the K(+)-dependent p-nitrophenylphosphatase activity by 25%, but it had no effect on Na(+)-dependent ATPase activity. The antibody partially inhibited the fluorescence changes of the enzyme labeled with 5'-isothiocyanatofluorescein after the addition of orthophosphate and Mg2+, and after the addition of ouabain. Proteolytic studies suggest that a part of the epitope is located on the cytoplasmic surface of the N-terminal half of the alpha-subunit. (Keyword) Animals / Antibodies, Monoclonal / Antigen-Antibody Complex / Fluorescein-5-isothiocyanate / Fluoresceins / Fluorescent Dyes / Kidney Medulla / Kinetics / Ligands / Macromolecular Substances / Peptide Fragments / Protein Conformation / Sodium-Potassium-Exchanging ATPase / Spectrometry, Fluorescence / Swine / Thiocyanates (Link to Publication Site) ● Publication site (DOI): 10.1016/0167-4838(90)90086-U (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 2169307 ● Search Scopus @ Elsevier (PMID): 2169307 ● Search Scopus @ Elsevier (DOI): 10.1016/0167-4838(90)90086-U (DOI: 10.1016/0167-4838(90)90086-U, PubMed: 2169307)
117.	Fusao Ota, Hirohisa Kato, Hideaki Nagamune, Yasuyuki Akiyama, Kuniko Seo, Yoshihiro Minato, Komei Fukui and Toshihiko Koga : Cross-reactive polysaccharide antigens (types a, d, and h) of the mutans group of streptococci: Different molecular forms of the type as distinguishable by monoclonal antibodies, Zentralblatt für Bakteriologie, Vol.272, No.3, 294-305, 1990. (Summary) ミュータンス群連鎖球菌S. cricetusの持つ血清型a，d及びh共通抗原には単クローン抗体で識別できる複数の分子型があることを証明した．本菌種の精製多糖体抗原には分子量46，9.8及び20KDaのAgI，AgII及びAgIIIが含まれ，AgIはMAba-4，AgIIとIIIはMAba-84により識別された．いずれも構成糖はラムノース，ガラクトース，グルコースだが各々の含有比は異なり，また抗原決定基構造も微妙な違いを持つことを示した．
118.	Fusao Ota, Katsuhiko Hirota, Hideaki Nagamune, Sonia Senesi, Yasuhiko Satomi, Masahiko Yasuoka, Yasuyuki Akiyama, Komei Fukui and Terushige Kawata : A comparison of competitive enzyme immunoassay and precipitin inhibition tests in the analysis of polysaccharide antigenic determinants of oral streptococci, Journal of Immunological Methods, Vol.124, No.2, 259-266, 1989. (Summary) 多糖体抗原の解析に用いる迅速·簡便な競合EIA法を開発した．従来から多糖体抗原の解析に標準的に用いられている免疫沈降法及びハプテンによる免疫沈降阻害法と比べて，多糖抗原固定化プレート上での競合EIA法は簡便性に優れ所要時間も短いことを示すとともにその最適実験条件も報告した．本法は単クローン抗体のような免疫沈降能が低いプローブを用いての，他の連鎖球菌の多糖体抗原の解析にも有用であることを結論づけた． (Link to Publication Site) ● Publication site (DOI): 10.1016/0022-1759(89)90362-1 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0024807150 (DOI: 10.1016/0022-1759(89)90362-1, Elsevier: Scopus)
119.	Hideaki Nagamune, Fusao Ota, Komei Fukui, Nagayuki Yoshida, Katsuhiko Hirota, Terushige Kawata and Yosihiro Minato : Studies on the immunological specificity of streptococci by use of monoclonal antibodies. .Selection of EIA microtiter plates for the immunological analysis of streptococcal whole cells, Shikoku Acta Medica, Vol.45, No.4, 256-264, 1989. (Summary) S. cricetusとS. sobrinus菌体を抗原とし，5社のEIAプレートの菌体固定化率を比較した結果，Corning社プレートが最も固定化効率が高い事を確認した．また，加熱，NaN3あるいはホルマリン処理をした菌体でもほぼ同一の固定化量を示し，同社プレートは菌体のEIA分析に最適である事を示した．さらに，菌体の固定化方法としては吸着乾固法が水中単純吸着法より効果的であることも示した． (Keyword) EIA(enzyme-immunoassay) / monoclonal antibody / streptococci / mutans group streptococci / EIA microtiter plate
120.	Fusao Ota, Hideaki Nagamune, Ken Nemoto, Komei Fukui, Nagayuki Yoshida, Katsuhiko Hirota, Michinori Kusuda, Terushige Kawata and Yosihiro Minato : Studies on the immunological specificity of streptococci by use of monoclonal antibodies. I.Selection of EIA plates and conditions for the use of polysaccharide antigens, Shikoku Acta Medica, Vol.45, No.4, 245-255, 1989. (Summary) 酵素免疫測定法(EIA)による多糖体抗原の分析条件の検討を行った．S. cricetusとS. sobrinus由来の多糖体を抗原に用い，5社のEIAプレートについての固定化効率を比較したところ，Corning社のプレートが最も吸着力が高かった．固定化方法としては吸着乾固法のほうが水中単純吸着法より効果的であった．また一次抗体や二次抗体の結合条件も検討し，水溶性の多糖体でも物理吸着法でEIA分析が可能なことを示した． (Keyword) EIA(enzyme-immunoassay) / Polysaccharide antigen / Streptococci / Monoclonal antibody
121.	Hideaki Nagamune, Osamu Urayama, Yukichi Hara, Fusao Ota, Katsuhiko Hirota, Yasuhiko Satomi, Kuniko Seo, Komei Fukui and Makoto Nakao : Directional immobilization of sodium- and potassium-activated ATPase to expose its cytoplasmic part to the liquid phase on microtiter plates by wheat germ agglutinin, Analytical Biochemistry: Methods in the Biological Sciences, Vol.180, No.2, 362-367, 1989. (Summary) 小麦胚芽凝集素(WGA)による糖蛋白質のEIAプレートへの固定化法を開発した．p-ベンゾキノン処理プレートへの直接的な固定化に比べ，WGAを介したNa,K-ATPaseの固定化効率は10倍以上高いことを確認した．またWGAにより固定化された酵素は基質サイトを液層側に配向して活性も保持していた．さらにWGAと親和性を持つ他の糖蛋白質でも高い固定化効率が確認され，この方法がWGA結合性糖蛋白質の有効な固定化法で有ることを証明した．
122.	Fusao Ota, Yoshihiro Minato, Sonia Senesi, Hideaki Nagamune, Yasuhiko Satomi, Hirohisa Kato, Yasuyuki Akiyama, Miwako Ono, Katsuhiko Hirota and Komei Fukui : Type-specific antigen of Streptococcus rattus strains (KAY1, FA1 and BHT) II. Ultrastructural localization, Zentralblatt für Bakteriologie, Vol.271, No.2, 137-145, 1989. (Summary) 特異抗血清を用い，ミュータンス群連鎖球菌S. rattusの血清型b特異的抗原の局在部位を免疫電顕法で解析した．S. rattusの全菌体及び細胞壁分画に対して血清型b特異抗血清を反応後，フェリチン標識化二次抗体で免疫標識したところ，いずれも細胞壁外部の不定型な物質に特異的なフェリチン顆粒が観察され，細胞壁上や細胞内部には顆粒が認められなかった．以上のことから血清型b特異的抗原が菌体外成分であることを形態学的に証明した．
123.	Fusao Ota, Hideaki Nagamune, Yasuyuki Akiyama, Hirohisa Kato, Yasuhiko Yasuoka, Miwako Ono, Yasuyori Morimoto, Komei Fukui, Yoshihiro Minato and Yoshio Takeda : Type-specific antigen of Streptococcus rattus strains (KAY1 and FA1). I. Characterization, Zentralblatt für Bakteriologie, Vol.271, No.1, 36-45, 1989. (Summary) ミュータンス群連鎖球菌S. rattusの血清型b特異的抗原の性状を解析した．イオン交換により得られた抗原分画の主成分は糖質と蛋白質であったが，抗原性はプロナーゼ，リパーゼ，ヌクレアーゼ処理には抵抗性を示した．また抗原を過ヨウ素酸酸化すると抗原性が失われ，免疫沈降ハプテン阻害実験の結果からも抗原にはガラクトースとグルコース残基が含まれていることが示唆された．これらを総合して，血清型b特異的抗原は糖成分であることを示した．
124.	Yasuo Shinohara, Hideaki Nagamune and Hiroshi Terada : The hydrophobic cationic cyanine dye inhibits oxidative phosphorylation by inhibiting ADP transport, not by electrophoretic transfer, into mitochondria, Biochemical and Biophysical Research Communications, Vol.148, No.3, 1081-1086, 1987. (Summary) シアニン色素tri-S-C4(5)はstate3呼吸及びATP合成を阻害した．しかしミトコンドリア逆転小胞に対しては他の疎水カチオンと同様に作用を示さなかった．さらにtri-S-C4(5)はアデニンヌクレオチド交換担体(ANT)によるADP輸送を阻害した．従って，この色素による酸化的リン酸化の阻害は，色素カチオンが電気泳動的にミトコンドリア内部に輸送されることによるのでなく，ANTの阻害が原因であると結論づけた．
125.	Yukichi Hara, Osamu Urayama, Kiyoshi Kawakami, Hiroshi Nojima, Hideaki Nagamune, Toshiyuki Kojima, Toshiko Ohta, Kei Nagano and Makoto Nakao : Primary structures of two types of alpha-subunit of rat brain Na+, K+-ATPase deduced from cDNA sequences, The Journal of Biochemistry, Vol.102, No.1, 43-58, 1987. (Summary) ラット脳のNa,K-ATPase触媒サブユニットのcDNAクローニングを行った．ウワバイン低感受性腎臓型サブユニットは，ウワバイン高感受性のヒト，ヒツジ，ブタのそれと97%，電気エイのそれとは87%の相同性を示した．ウワバイン低感受性及び高感受性酵素間で細胞外ドメインの構造を比べたところ，わずか2残基のみの置換が見られた．さらにラット腎型サブユニットとは85%の相同性を示す未知のαサブユニットも見いだした．
126.	Masaki Ito, Masamichi Nakamura, Hideaki Nagamune, Norifumi Morikawa and Hiroshi Terada : An Escherichia coli mutant exhibiting temperature-sensitive ATP synthesis, Biochemical and Biophysical Research Communications, Vol.138, No.1, 72-77, 1986. (Summary) 温度感受性Unc形質を示す大腸菌変異株SM434(tte-3)の性質を解析した．ttr-3変異はilvA遺伝子とのリンクは無かったが，大腸菌ゲノム81分-91分によって相補された．また，本株はN,N'-ジシクロヘキシルカーボジイミド耐性を示し，細胞増殖の場合と同程度の温度感受性形質をATP合成において示した．これらの結果から，本株がエネルギー転換系を担う未知の遺伝子において変異(ttr-3 )を持つ可能性を示した．
127.	Hideaki Nagamune, Osamu Urayama, Yukichi Hara and Makoto Nakao : Characterization of ouabain-sensitive phosphatase activity in the absence of potassium ion in purified pig kidney Na, K-ATPase, The Journal of Biochemistry, Vol.99, No.6, 1613-1624, 1986. (Summary) 豚腎Na,K-ATPaseにおいて，K+非存在下にウワバイン感受性ホスファターゼ活性を解析したところ，この活性はこの酵素分子によって触媒されていた．本酵素の基質となるADP，ATP，無機リン酸などはホスファターゼ反応に対する競合阻害剤となり，そのKi値は本酵素における各基質の高親和性サイトへの親和性に相当した．また蛍光法による酵素コンフォメーションの解析から，このホスファターゼ反応はE1->E2->E1の循環反応であることも示した．
128.	Osamu Urayama, Hideaki Nagamune, Makoto Nakao, Yukichi Hara, Hiroyuki Sugiyama, Kanako Sato and Toshiko Nakao : Isolation and characterization of a monoclonal antibody against pig kidney sodium- and potassium-activated ATPase, The Journal of Biochemistry, Vol.98, No.1, 209-217, 1985. (Summary) 豚腎Na,K-ATPaseに対する単クローン抗体mAb38を調製した．この抗体は変性酵素には結合せず，未変性の酵素を高い親和性(半飽和結合濃度=0.6nM)で認識することがEIA及び免疫親和性クロマトグラフィーで示された．酵素と抗体の結合比は1:0.86であった．無傷赤血球に対しては特異結合が認められたが，その反転小胞には特異結合せず，mAb38がNa,K-ATPaseの細胞表面部位に対する抗体であることを示した．
129.	Hiroshi Terada, Hideaki Nagamune, Hidefumi Morikawa and Masumi Ikuno : Uncoupling of oxidative phosphorylation by divalent cationic cyanine dye. Participation of phosphate transporter, Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol.807, No.2, 168-176, 1985. (Summary) The trinuclear cationic cyanine dye tri-S-C4(5) was found to be an uncoupler of oxidative phosphorylation. Its uncoupling required inorganic phosphate (Pi) or arsenate, which is transported into mitochondria via the Pi transport system, and was abolished by the Pi-transport inhibitor N-ethylmaleimide or mersalyl. The dye stimulated Pi uptake into mitochondria, and its uncoupling action was accompanied by swelling of the mitochondria. The adenine nucleotides ADP and ATP protected mitochondria from uncoupling by the dye. The dye taken up by mitochondria was released into the incubation medium on induction of uncoupling. In the absence of Pi, the dye did not cause uncoupling, but its uptake was much greater than in the presence of Pi. The cyanine dye is suggested to induce uncoupling by acting on the membrane, rather than after its electrophoretic transfer into the mitochondria. (Keyword) Adenine Nucleotides / Animals / Carrier Proteins / Coloring Agents / Male / Mitochondria / Onium Compounds / Organophosphorus Compounds / Oxidative Phosphorylation / Phosphate-Binding Proteins / Phosphates / Rats / Rats, Inbred Strains / Thiazoles / Uncoupling Agents (Link to Publication Site) ● Publication site (DOI): 10.1016/0005-2728(85)90120-3 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 3978093 ● Search Scopus @ Elsevier (PMID): 3978093 ● Search Scopus @ Elsevier (DOI): 10.1016/0005-2728(85)90120-3 (DOI: 10.1016/0005-2728(85)90120-3, PubMed: 3978093)
130.	Hideaki Nagamune, Hiroshi Terada and 亀谷富士夫 : ミトコンドリア機能に対するシアニン色素triS-C4(5) (NK-24)の2元的効果, 感光色素, Vol.89, 19-25, 1984. (Summary) triS-C4(5)は無機リン酸依存的にミトコンドリア酸化的リン酸化を脱共役するが，より低濃度では呼吸促進なしにATP合成を阻害することを見いだした．その濃度域では，本色素のミトコンドリアへの結合は既に飽和していたが内膜膜電位は消失しておらず，またATP分解活性の上昇も見られなかった．従ってこの色素によるATP合成阻害は，内膜電位の消失が原因ではなく，ATP合成酵素かADP/ATP交換担体の阻害が原因である可能性を示した．
131.	Osamu Urayama, Motoko Nakao, Hideaki Nagamune and Hiroyuki Sugiyama : Coupling of membrane-bound and detergent-solubilized sodium- and potassium-activated ATPase to p- benzoquinone-treated microtiter plates, Analytical Biochemistry: Methods in the Biological Sciences, Vol.141, No.1, 194-198, 1984. (Summary) 膜結合型と可溶化型のNa,K-ATPaseを，p-ベンゾキノンによりEIAプレートに共有結合で固定化し，本酵素に対する単クローン抗体によりその固定化率を比較した結果，可溶化型は膜結合型より2-3倍の高効率で固定化されることが判明した．また別の可溶化膜蛋白質(赤血球のBand3)も同方法により高い効率で固定化できることが示され，p-ベンゾキノン固定化法は洗剤の存在下でも効果的な蛋白質固定化法として有用である事を示した．
132.	Hiroshi Terada, Hideaki Nagamune, Norifumi Morikawa and Tetsuo Ichikawa : The cyanine dye triS-C4(5) as a cationic uncoupler of oxidative phosphorylation: Interaction with mitochondria detected by derivative spectrophotometry, Cell Structure and Function, Vol.8, No.2, 161-170, 1983. (Summary) 微分吸収スペクトル法を用いてtri-S-C4(5)のミトコンドリアへの結合と，脱共役作用及びミトコンドリア体積膨潤作用を経時的に比較した．その結果，無機リン酸イオン存在下で脱共役状態のミトコンドリアに対してよりも，無機リン酸イオン非存在下で共役状態のミトコンドリアへの結合量が大幅に多く，しかもその際にミトコンドリアは収縮しており，tri-S-C4(5)がミトコンドリア内に蓄積する事が脱共役作用の原因では無いことを分光学的に示した．
133.	Hiroshi Terada and Hideaki Nagamune : A cyanine dye tri-S-C₇(5) Phosphate-dependent cationic uncoupler of oxidative phosphorylation in mitochondria, Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol.723, No.1, 7-15, 1983. (Summary) The trinuclear cyanine dye, tri-S-C7(5), at about 10 microM stimulated State 4 respiration of rat liver mitochondria more than 6-fold and released oligomycin-inhibited respiration completely. Thus, the dye is concluded to be a very effective cationic uncoupler of oxidative phosphorylation in mitochondria. However, for exhibition of its uncoupling action, the presence of Pi (or arsenate) was necessary, and a phosphate-transport inhibitor, N-ethylmaleimide or mersalyl, inhibited its action. The stimulation of phosphate transport via the Pi carrier by the dye is suggested to be directly related to the uncoupling action. (Keyword) Animals / Ethylmaleimide / Male / Mersalyl / Mitochondria, Liver / Oxidative Phosphorylation / Oxygen Consumption / Phosphates / Rats / Rats, Inbred Strains / Thiazoles / Uncoupling Agents (Link to Publication Site) ● Publication site (DOI): 10.1016/0005-2728(83)90003-8 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 6830769 ● Search Scopus @ Elsevier (PMID): 6830769 ● Search Scopus @ Elsevier (DOI): 10.1016/0005-2728(83)90003-8 (DOI: 10.1016/0005-2728(83)90003-8, PubMed: 6830769)
134.	Hiroshi Terada, Hideaki Nagamune, Yumi Osaki and Kenichi Yoshikawa : Specific requirement for inorganic phosphate for induction of bilayer membrane conductance by the cationic uncoupler carbocyanine dye, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.646, No.3, 488-490, 1981. (Summary) The trinucleous divalent cationic cyanine dye triS-C4(5) was shown to be an uncoupler of oxidative phosphorylation in mitochondria only in reaction medium containing inorganic phosphate (Pi). This dye also induced marked increase in the electrical conductance of a phospholipid bilayer membrane in bathing solution containing Pi, but not in solution containing Tris-HCl buffer without Pi. Time-dependent fluctuation of the electrical current across the bilayer membrane was observed in the presence of triS-C4(5) only in bathing solution containing Pi. This fluctuation could be due to perturbation of the bilayer membrane structure induced by the cooperative action of the cyanine dye and Pi, and this perturbation should be directly related to their effects in increasing membrane conductance and also causing uncoupling in mitochondria. (Keyword) Animals / Coloring Agents / Electric Conductivity / Lipid Bilayers / Mitochondria, Liver / Oxidative Phosphorylation / Oxygen Consumption / Phosphates / Rats / Thiazoles / Uncoupling Agents (Link to Publication Site) ● Publication site (DOI): 10.1016/0005-2736(81)90320-5 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 7284375 ● Search Scopus @ Elsevier (PMID): 7284375 ● Search Scopus @ Elsevier (DOI): 10.1016/0005-2736(81)90320-5 (DOI: 10.1016/0005-2736(81)90320-5, PubMed: 7284375)
135.	Hiroshi Terada, Hideaki Nagamune and Fujio Kametani : Effect of anions on the uncoupling of the cyanine dye, Platonin (NK-19), Medical Research of Photosensitizing Dyes, Vol.88, 1-9, 1981. (Summary) 二価カチオン性シアニン色素tri-S-C7(5)の無機リン酸に依存した脱共役作用は，この色素と無機リン酸のイオン対がミトコンドリア内に移行して発現している可能性が考えられた．そこでこの色素の対イオンとなりうる様々なミトコンドリア膜透過性のアニオン存在下で，分配係数やミトコンドリア呼吸促進作用を比較し，tri-S-C7(5)による脱共役作用は無機リン酸とのイオン対形成によるミトコンドリア膜内移行が原因では無いことを示した

Academic Paper (Unrefereed Paper):

1.	Fumitoshi Yagishita, Keita Hoshi, Atsushi Tabata, Hideaki Nagamune, Keiji Minagawa and Yasushi Imada : Fluorescent Imidazo[1,2-a]pyridinium Salt for Potent Cancer Therapeutic Agent, Proceedings of the LED General Forum 2021 Tokushima, 155-156, 2021.
2.	Kawasaka Kaito, Hideaki Nagamune and Akihiro Shirai : フェノール酸とブルーライトを併用した真菌の光不活性化, LED総合フォーラム 2021 in 徳島論文集, Vol.P-6, 149-150, 2021. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1010853567258226176 (CiNii: 1010853567258226176)
3.	Fumitoshi Yagishita, Tanigawa Junichi, Sanagawa Yohei, Okamoto Masaki, Ishihara Kaito, Atsushi Tabata, Hideaki Nagamune, Yasushi Imada and Yasuhiko Kawamura : Fluorescent N-Heteroarene as a DNA Photo-cleaving Agent under the LED Irradiation, LED総合フォーラム2019 in 徳島論文集, Vol.2019, 71-72, 2019.
4.	Kunimi Haruka, Hideaki Nagamune and Akihiro Shirai : フェルラ酸と紫外線-Aの酵母に対する併用殺菌機構の解析, LED総合フォーラム 2019 in 徳島論文集, Vol.P-6, 67-68, 2019.
5.	Kunimi Haruka, Hideaki Nagamune and Akihiro Shirai : フェルラ酸と紫外線-Aの酵母に対する併用殺菌機構とそのポストハーベスト保存法としての検討, LED総合フォーラム 2018 in 徳島論文集, Vol.P-16, 119-124, 2018.

Academic Letter:

1.	Hideaki Nagamune, Akiko Sukeno, Robert A. Whiley, Syed I. Jafar, Joseph Aduse-Opoku, Kazuto Ohkura, Katsuhiko Hirota, Yoichiro Miyake, Takuya Maeda and Hiroki Kourai : Intermedilysin plays a key role in the invasion of human cells by Streptococcus intermedius, Streptococci New Insights into an Old Enemy: International Congress Series, Vol.1289, 262-266, 2006. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.ics.2005.11.021 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-33645897338 (DOI: 10.1016/j.ics.2005.11.021, Elsevier: Scopus)
2.	Ayuko Takao, Hideaki Nagamune and Nobuko Maeda : Hyaluronate lyase and sialidase in the anginosus streptococci: As the putative virulence factors, Streptococci New Insights into an Old Enemy: International Congress Series, Vol.1289, 231-234, 2006. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.ics.2005.10.035 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-33645954296 (DOI: 10.1016/j.ics.2005.10.035, Elsevier: Scopus)
3.	Hisashi Ohkuni, Yuko Todome, Hidemi Takahashi, Hideaki Nagamune, Jun Abe, Hiroki Ohtsuka and Hisashi Hatakeyama : Antibody titers to Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) in the sera of patients with Kawasaki disease, Streptococci New Insights into an Old Enemy: International Congress Series, Vol.1289, 71-74, 2006.

Review, Commentary:

1.	Toshifumi Tomoyasu and Hideaki Nagamune : Functional comparison between the DnaK chaperone systems of Streptococcus intermedius and Escherichia coli, STRESS AND ENVIRONMENTAL REGULATION OF GENE EXPRESSION AND ADAPTATION IN BACTERIA, Vol.2, 791-795, Jun. 2016. (Keyword) Streptococcus / Escherichia coli / molecular chaperone (Link to Publication Site) ● Publication site (DOI): 10.1002/9781119004813.ch76 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85018833367 (DOI: 10.1002/9781119004813.ch76, Elsevier: Scopus)
2.	Hideaki Nagamune : アンギノーサス群連鎖球菌の病原因子, 日本細菌学雑誌, Vol.63, No.2-4, 425-435, Jun. 2008.
3.	Ayuko Takao, Hideaki Nagamune and Nobuko Maeda : The hyaluronate lyase in Streptococcus intermedius and Streptococcus constellatus: A putative virulence factor, Recent Research Development in Microbiology, Vol.9, 81-96, Feb. 2005.
4.	Hideaki Nagamune, Kazuto Ohkura and Hisashi Ohkuni : Molecular bases of group A streptococcal pyrogenic exotoxin B, Journal of Infection and Chemotherapy, Vol.11, No.1, 1-8, Feb. 2005. (Summary) 猩紅熱や連鎖球菌毒素ショック症候群を起こすことが知られるA群連鎖球菌(GAS)が分泌するプロテアーゼ性外毒素B(speB)について，speBがGAS感染症の成立や進展において果たす臨床的な意義を病理学的に論じた．さらにその分子特性，立体構造，またspeB遺伝子発現調節機構等のタンパク化学的及び分子生物学的な特質についても解説を行った． (Link to Publication Site) ● Publication site (DOI): 10.1007/s10156-004-0354-y (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1007/s10156-004-0354-y (DOI: 10.1007/s10156-004-0354-y)
5.	Hideaki Nagamune and Hitoshi Matsuki : 遺伝子治療及び癌の免疫療法への応用に向けての細胞膜機能改変技術の開発, Bulletin of Faculty of Engineering, The University of Tokushima, Vol.48, 83-89, Jan. 2003. (Summary) 微生物に由来し，細胞膜に結合する細胞溶解毒素の無毒性部分構造を利用して，さまざまな生理活性物質をそれと連結体として細胞膜に結合させ，細胞膜の機能や性質を変化させる技術について論じた．その一例として，連鎖球菌由来のインターメディリシン(ILY)のドメイン4に胎児性癌抗原(CEA)に対する抗体の(Fab')2断片を連結し，これを細胞膜に結合させた赤血球をCEA陽性の癌細胞TTに標的化するシステムを開発し，この技術が様々な細胞を標的とした遺伝子治療や癌の免疫療法に活用できる有用な技術であることを示した．
6.	Hideaki Nagamune : 連鎖球菌の細胞溶解毒素, Seikagaku, Vol.69, No.5, 343-348, May 1997. (Summary) 連鎖球菌属には，猩紅熱などを起こすA群溶血性連鎖球菌や薬剤耐性化しやすい肺炎球菌を筆頭に，様々な全身性或いは局所感染症を起こす菌種や菌群が知られており，現在もなお病原微生物として重要視されている．ここでは最近の分子論的知見を基に，その病原因子として働くストレプトリシンOなどの細胞溶解毒素の構造や機能を説明し，著者が見いだしたヒト細胞に特異的なインターメディリシンについても特徴を解説した．
7.	Hideaki Nagamune : 病原性大腸菌O157:感染予防のために, STEPテクノ情報, Vol.2, No.3, 12-17, Dec. 1996. (Summary) 近年，社会問題になった腸管出血性大腸菌O157:H7と，それと同様にベロ毒素を分泌して中毒を起こす類縁の大腸菌について解説した．これらの細菌によって中毒の起こるメカニズムや中毒症状，また岡山県邑久町や大阪堺市や起こった集団食中毒事件も例にとって流行の実態を分かりやすく解説した．また腸管出血性大腸菌O157:H7による感染から身を守る具体的な対処方法や最新の疫学情報などが得られるインターネットホームページも紹介した．
8.	Yoshiko Matsuda, Akihiko Tsuji, Hideaki Nagamune, Tetsuya Akamatsu, Chiemi Hine, Kazuaki Muramatsu, Kenji Mori, Yasuhiro Tamai and Kayoko Yonemoto : Novel members of mammalian Kexin family proteases, PACE4C, PACE4D, PC7A and PC7B., Advances in Experimental Medicine and Biology, Vol.389, 63-71, Sep. 1996. (Summary) ペプチドホルモンや増殖因子等は不活性な前駆体として合成され, 特定の塩基性アミノ酸部位での限定切断を経て初めて活性化される. Kexin family proteaseはこのプロセシングを担う重要な酵素であり, 本研究では我々が新規メンバーをクローニングし, その構造, 組織分布等を明らかにした. (Keyword) Processing protease
9.	Yoshiko Matsuda, Akihiko Tsuji, Hideaki Nagamune, Tetsuya Akamatsu, Chiemi Hine, Kazuaki Muramatsu, Kenji Mori, Yasuhiro Tamai and Kayoko Yonemoto : Novel members of mammalian Kexin family proteases, PACE4C, PACE4D, PC7A and PC7B, Intracellular protein catabolism, Vol.389, No.7, 63-71, Jan. 1996.
10.	加藤裕久, Fusao Ota, Hideaki Nagamune and 福井公明 : 単クローン抗体を用いたStreptococcus mutansの研究, 微生物, Vol.4, No.3, 252-259, Mar. 1988. (Summary) 単クローン抗体は特異性や反応の均一性に優れ，微生物領域では様々な病原体の検出·同定に凄まじい威力を発揮している．ここではう蝕や亜急性心内膜炎の原因となるS. mutansの菌種や血清型の同定，菌体表層の蛋白質及び多糖体抗原の研究，また菌体が歯面に定着する際に重要となるムタンを形成するグルコシルトランスフェラーゼの研究などに単クローン抗体が応用された例を解説し，その有用性を議論した．
11.	Hideaki Nagamune and 中尾眞 : Na, K-ATPaseの生化学, The Journal of Clinical Science, Vol.22, No.10, 1273-1280, Oct. 1986. (Summary) 腎尿細管や神経細胞膜などに多く存在するNa, K-ATPaseは，ATPの水解エネルギーを使って細胞内のNa+を汲み出し細胞外のK+を取り込む細胞膜酵素であり，この酵素の機能を利用して原尿の再吸収や神経刺激伝達が制御されている．この重要な酵素について，構成サブユニットの構造や，様々なイオンによって複雑に制御される酵素反応機構などを当時の最新の知見を基に分かりやすく説明し，この酵素の阻害剤についても解説を加えた．
12.	Hiroshi Terada and Hideaki Nagamune : シアニン色素のミトコンドリアに対する作用 -微分スペクトル法の生体膜への応用-, 島津科学機械ニュース, Vol.22, No.5, 20-23, May 1981. (Summary) 細胞やオルガネラの共存下に特定の物質の吸光スペクトルを測定する場合，測定系に含まれる生体試料の体積変化や会合などに伴う濁度変化を補正する必要がある．しかし経時的にそのベースラインが不規則に変動する場合は測定が困難である．ところが微分吸光スペクトルを採ればベースラインの変動をほぼ完全に除去することが可能であり，ここでは体積変化を伴うミトコンドリアへの色素結合反応を例にとってその原理と実際を解説した．

Proceeding of International Conference:

1.	Kaito Kawasaka, Hideaki Nagamune, Koichiro Tsuchiya and Akihiro Shirai : Investigation of photoinactivation mechanism of fungal conidia using blue light in combination with phenolic acids, 2021 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2021), Vol.3417683, Honolulu, Dec. 2021.
2.	Nakai Hiroki, Akiyama Daiu, Taniguchi Yoshiaki, Kishinobu Iori, Ikeda Takuya, Atsushi Tabata, Hideaki Nagamune, Yasuhide Ohno and Masao Nagase : Protein detection by electron donor using epitaxial graphene film on SiC substrate, 33rd International Microprocesses and Nanotechnology Conference (MNC2020), No.2020-21-12, Online, Nov. 2020.
3.	Fumitoshi Yagishita, Sanagawa Yohei, Tanigawa Jun-ichi, Nii Chiho, Atsushi Tabata, Hideaki Nagamune, Yasushi Imada and Yasuhiko Kawamura : Synthesis of Water-Soluble Fluorescent N-Heteroarenes And Its Applications, 18th International Symposium on Novel Aromatic Compounds (ISNA-18), Jul. 2019.
4.	Yamada Takuya, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Response of human cells against peptide type hemolysin Streptolysin S derived from Streptococcus anginosus subsp. anginosus, International Peptide Symposium, Dec. 2018. (Keyword) Streptococcus / cytolysin / peptide
5.	Tang Qing, Masayoshi Onitsuka, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Characterizatio of anti-HER2 scFv and Fab as a targeting module for novel drug-delivery system against HER2-positive cancer, 24th IUBMB congress & 15th FAOBMB congress, Seoul, Korea, Jun. 2018.
6.	Toshifumi Tomoyasu, Atsushi Tabata, Chiba Shinya, Yamasaki Takahiro, Kusaka Shingo, Takeda Nozomi, Tamaoka Masaaki, Kazuto Ohkura, Whiley A. Robert and Hideaki Nagamune : Positive and negative control mechanisms of pathogenicity expression in Streptococcus intermedius, Lancefield International Symposium on Streptococci and Streptococcal Diseases, Denarau Island Fiji, Oct. 2017. (Keyword) pathogenic microorganism / bacterial toxins / immunity
7.	Atsushi Tabata, Deutsch Douglas, Otsuka Seiya, Verratti Kathleen, Toshifumi Tomoyasu, Hideaki Nagamune and Fischetti A. Vincent : Characterization of a novel plasmid discovered in a clinical isolate of Streptococcus anginosus subsp. anginosus, Lancefield International Symposium on Streptococci and Streptococcal Diseases, Denarau Island Fiji, Oct. 2017. (Keyword) pathogenic microorganism / plasmid
8.	Toshifumi Tomoyasu, Shinya Chiba, Takahiro Yamasaki, Shingo Kusaka, Atsushi Tabata and Hideaki Nagamune : Expression control pathways of ily by blood components in Streptococcus intermedius, The 13th Korea - Japan International Symposium on Microbiology (XIII-KJISM), May 2016. (Keyword) cytolysin / sugar / antibody / Streptococcus
9.	Masashi Yamamoto, Reiko Matsumura, Yoshihiko Hirata and Hideaki Nagamune : Evaluation of biocompatibility of novel and commonly-used antiseptics by cell culture method, Antimicrobial Resistance and Infection Control, Vol.4, No.suppl1, 38, Geneva, Switzerland, Jun. 2015. (Keyword) antiseptics / biocompatibility
10.	Hideaki Nagamune, Atsushi Tabata, Yoshitoshi Ogura and Tetsuya Hayashi : Diversity in genome structure showing the genomic dynamism of human-derived Streptococcus mitis, International Symposium on Genome Science 2015: Expanding Frontiers of Genome Science II, Jan. 2015. (Keyword) Streptococcus mitis / genome science
11.	Takao Ayuko, Hideaki Nagamune and Maeda Nobuko : INFLUENCE OF SIGNAL PEPTIDE PHEROMONES ON TRANSFORMATION EFFICIENCY AND BACTERIOCIN PRODUCTION IN STREPTOCOCCUS INTERMEDIUS, Proceeding of International Conference/XIX Lancefield International Symposium on Streptococci and Streptococcal Diseases, Nov. 2014. (Keyword) PHEROMONE / BACTERIOCIN / STREPTOCOCCUS INTERMEDIUS / COMPTENCE-STIMULATING PEPTIDEE / BACTERIOCIN-INDUCING PEPTIDE
12.	Hideaki Nagamune, Toshifumi Tomoyasu, Hidenori Imaki, Naoki Yamamoto, Chiharu Taue, 増田早智子, 高尾亞由子, 前田伸子, Atsushi Tabata and Whiley A. Robert : CHARACTERIZATION OF A NOVEL SECRETED GLYCOSIDASE WITH MULTIPLE GLYCOSIDASE ACTIVITIES, MSGA: A CANDIDATE KEY ENZYME REGULATING GROWTH AND PATHOGENICITY OF STREPTOCOCCUS INTERMEDIUS, XIX Lancefield International Symposium on Streptococci and Streptococcal Diseases, Nov. 2014. (Keyword) Streptococcus / enzyme / gene expression / cytolysin
13.	Atsushi Tabata, Kota Nakano, Yuji Sato, Kentaro Maya, Hiromi Ohtani, Yukimasa Ohkubo, Kazuto Ohkura, Ken Kikuchi, Robert A Whiley, Toshifumi Tomoyasu and Hideaki Nagamune : STREPTOLYSIN S HOMOLOGUES OF PEPTIDE HEMOLYSIN: NOVEL BETA-HEMOLYTIC FACTORS DISTRIBUTED AMONG BETA-HEMOLYTIC ANGINOSUS GROUP STREPTOCOCCI, XIX Lancefield International Symposium on Streptococci and Streptococcal Diseases, Buenos Aires, Argentina, Nov. 2014. (Keyword) Streptococcus / pathogenic microorganism / virulence factor
14.	Ayuko Takao, Hideaki Nagamune and Nobuko Maeda : Competence-specific sigma factor genes in the anginosus streptococci, IUMS 2011 Sapporo Congress, Sapporo, Sep. 2011.
15.	Yukihiro Nishikawa, Atsushi Tabata, Toshifumi Tomoyasu, Momoyo Azuma, Yasuhiko Nishioka, Saburo Sone and Hideaki Nagamune : Triple fusion PCR immunochromatography for a rapid and simple diagnosis of penicillin-resistant Streptococcus pneumoniae, IUMS 2011 Sapporo Congress, Sapporo, Japan, Sep. 2011. (Keyword) Streptococci / antibiotics / clinical laboratory testing
16.	Kota Nakano, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Investigation of the novel cytotoxic factor secreted from streptococcus anginosus, IUMS 2011 Sapporo Congress, Sapporo, Sep. 2011.
17.	Hidenori Imaki, Toshifumi Tomoyasu, Sachiko Masuda, Atsushi Tabata and Hideaki Nagamune : Molecular and phenotypic analysis of mutants of ily expressional control factors in Streptococcus intermedius, IUMS 2011 Sapporo Congress, Palermo, Italy, Sep. 2011. (Keyword) Streptococci / cytolysin / gene expression
18.	Atsushi Tabata, Yasuhiro Yamamoto, Ayuko Takao, Toshifumi Tomoyasu, Ayako Nakayama, Nobuko Maeda, Hisashi Ohkuni and Hideaki Nagamune : Investigation of pathogenic properties of cholesterol-dependent cytolysin producing Streptococcus mitis, XVIII Lancefield International Symposium, Palermo, Italy, Sep. 2011. (Keyword) Streptococci / cytolysin
19.	Toshifumi Tomoyasu, Sachiko Masuda, Hidenori Imaki, Atsushi Tabata and Hideaki Nagamune : Genetic screening of ily expression control factors in Streptococcus intermedius, XVIII Lancefield International Symposium, Palermo, Italy, Sep. 2011. (Keyword) Streptococci / cytolysin / gene expression
20.	Makoto Nishimoto, Masaki GOTO, Nobutake Tamai, Hideaki Nagamune, Shoji Kaneshina and Hitoshi Matsuki : Effect of pressure on interactions of anti-fluorescent probe monoclonal antibody with a ligand and inhibitors, Journal of Physics: Conference Series, Vol.215, No.1, 12157, Bristol, Mar. 2010. (Link to Publication Site) ● Publication site (DOI): 10.1088/1742-6596/215/1/012157 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012157 (DOI: 10.1088/1742-6596/215/1/012157)
21.	Susilowati Heni, Hirohiko Okamura, Katsuhiko Hirota, Kaya Yoshida, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : Nuclear Translocation of NF-kB induced by Streptococcus intermedius intermedilysin, International Conference of Indonesian Society for Microbiology: Recent advances of Microbiology in Health, Agriculture, Bioindustry, Surabayai, Nov. 2009.
22.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Functions of DnaK chaperone system in Streptococcus intermedius, Cell Stress Society International, Sapporo, Oct. 2009. (Keyword) pathogenic microorganism / virulence factor
23.	Susilowati Heni, Hirohiko Okamura, Katsuhiko Hirota, Kaya Yoshida, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : The molecular mechanism for Streptococcus intermedius intermedilysin-induced cell death inhuman cholangiocellular carcinoma cells, International Joint Symposium Frontier in Biomedical Sciences: From Genes to Applications, Yogyakarta, Nov. 2008.
24.	Hiromichi Yumoto, Liu Dali, Keiji Murakami, Katsuhiko Hirota, Hideaki Nagamune, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake : Gene-regulatory and Physiological Functions of Histone-like Protein from Streptococci, 86th General Session and Exhibition of the International Association for Dental Research, Toronto (Canada), Jul. 2008.
25.	Atsushi Tabata, Shizuka Saito, Eriko Sakakura, Yuki Shinohara, Soichiro Miyoshi, Kou Fukushima, Toshifumi Tomoyasu, Hisashi Ohkuni and Hideaki Nagamune : Streptococcus mitis-derived human platelet aggregation factor is a novel type of cholesterol-dependent cytolysin that recognizes dual receptors, cholesterol and human CD59., XVII Lancefield International Symposium on Streptococci & Streptococcal Diseases, Porto Heli, Greece, Jun. 2008.
26.	Susilowati Heni, Hirohiko Okamura, Katsuhiko Hirota, Kaya Yoshida, Keiji Murakami, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : NFAT1 activation in intermedilysin-induced human cholangiocellular carcinoma cell HuCCT1, The 2nd International Symposium on "The Future Direction of Oral Sciences in the 21st Century", Tokushima, Dec. 2007.
27.	Heni Susilowati, Katsuhiko Hirota, Hirohiko Okamura, Kaya Yoshida, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : Intermedilysin induces cell death in HepG2 cells, 21st International Association for Dental Research - South East Asia Division, Bali, Indonesia, Sep. 2007.
28.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Function of DnaK in Streptococcus intermedius, Cell Stress Society International, Budapest, Aug. 2007. (Keyword) Streptococcus / Chaperon / DnaK
29.	Heni Susilowati, Katsuhiko Hirota, Hirohiko Okamura, Kaya Yoshida, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : The mechanisms of Intermedilysin to induce cell death on HepG2 and HuCCT1 cell lines, The 1st international symposium and workshop ''The Future Direction of Oral Sciences in the 21st century'', Awajishima, Mar. 2007.
30.	Atsushi Tabata, Akiko Inubushi, Toshifumi Tomoyasu and Hideaki Nagamune : Immunochemical analysis of a human specific cytolysin, intermedilysin, for elucidation of its mode of action to human cells, 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Kyoto, Jun. 2006.
31.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Small heat shock protein ``AgsA'' suppresses aggregation of heat denatured proteins., 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Kyoto, Jun. 2006.
32.	Ayuko Takao, Hideaki Nagamune and Nobuko Maeda : Hyaluronate lyase and sialidase in the Anginosus streptococci:Putative virulence factor, XVIth Lancefield International Symposium on Streptococci and Streptococcal Diseases: In "Streptococci-New insights into an old enemy", International Congress Series, Vol.1289, 231-234, Palm cove, Australia, Apr. 2006.
33.	Hisashi Ohkuni, Yuko Todome, Hidemi Takahashi, Hideaki Nagamune, Jun Abe, Hiroki Ohtsuka and Hiroshi Hatakeyama : Antibody titers to Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) in the sera of patients with Kawasaki disease, XVIth Lancefield International Symposium on Streptococci and Streptococcal Diseases: In "Streptococci-New insights into an old enemy", International Congress Series, Vol.1289, 71-74, Palm cove, Australia, Apr. 2006.
34.	Hideaki Nagamune, Akiko Sukeno, Robert A. Whiley, Syed I. Jafar, Joseph Aduse-Opoke, Kazuto Ohkura, Takuya Maeda, Katsuhiko Hirota, Yoichiro Miyake and Hiroki Kourai : Intermedilysin plays a key role in the invasion of human cells by Streptococcus intermedius, XVIth Lancefield International Symposium on Streptococci and Streptococcal Diseases: In "Streptococci-New insights into an old enemy", International Congress Series, Vol.1289, 262-266, Palm cove, Australia, Sep. 2005.
35.	Graeme Cowan, Hideaki Nagamune and Timothy Mitchell : The human-specificity of intermedilysin is determined by the last 56 residues of domain 4, The 5th Workshop on Pore-forming Toxins, Mainz, Germany, Oct. 2004.
36.	Hideaki Nagamune, Hisashi Ohkuni, Kazuto Ohkura, Takuya Maeda and Hiroki Kourai : Streptococcus mitis-derived human platelet aggregation factor secreted by an isolate from a Kawasaki disease patient is a unique member of cholesterol-dependent cytolysin, The 5th Workshop on Pore-forming Toxins, Mainz, Germany, Oct. 2004.
37.	Hiromichi Yumoto, Katsuhiko Hirota, Hideaki Nagamune, Kazuto Ohkura, Keiji Murakami, Masayoshi Yamada, Hideaki Nakae, Yoichiro Miyake and Takashi Matsuo : Molecular analysis of histone-like protein from Streptococcus intermedius, 80th General Session and Exhibition of the International Association for Dental Research, San Diego(USA), Mar. 2002.
38.	Hideaki Nagamune, Takatsugu Goto, Robert A. Whiley, Takuya Maeda, Yasuko Inai, Ohki Ohnishi, Kazuto Ohkura, Jeremy M. Hardie and Hiroki Kourai : Structural and epidemiological characterization of a human-specific cytolysin, intermedilysin: A potentially significant virulence factor in Streptococcus intermedius infections, XIV Lancefield International Symposium on Streptococci and Streptococcal Diseases; In "Streptococci and Streptococcal Diseases"(D. R. Martin, J. R. Tagg, eds.), 737-740, Auckland, Sep. 2000. (Summary) クローニングされたヒト特異的細胞溶解毒素インターメディリシン(ILY)遺伝子を発現する大腸菌ではヒト特異的溶血反応が確認され，クローン化遺伝子が活性型であることを確かめた．またその遺伝子情報に基づいたPCRによるily遺伝子分布検索の結果，アンギノーサス群連鎖球菌におけるily遺伝子の分布はS. intermedius(SI)のみに限定されていた．しかもヒト口腔内に常在する株に比べて重篤な深部膿瘍からの分離株の場合にはILY分泌量がタンパク質ベースで見た場合でも6-10倍も多いことから，SI感染症にILYが重要な病原因子として働くことを疫学的に示した．
39.	Kazuto Ohkura, Takatsugu Goto, Kanako Hattori, Ohki Ohnishi, Hideaki Tsuge, Nobuhiko Katunuma, Katsuhiko Hirota, Yoichiro Miyake, Hiroki Kourai and Hideaki Nagamune : Structural Analysis of a Human Specific Cytolysin Intermedilysin from Streptococcus intermedius UNS46, 18th International Congress of Biochemistry and Molecular Biology, Birmingham, UK, Jul. 2000.
40.	Hideaki Nagamune, Ohki Ohnishi, Kanako Hattori, Kazuto Ohkura, Takatsugu Goto, Hideaki Tsuge, Katsuhiko Hirota, Nobuhiko Katunuma, Yoichiro Miyake, Takuya Maeda and Hiroki Kourai : Human-Specific Cell Lysis by Intermedilysin, an Exotoxin of Streptococcus intermedius, 18th International Congress of Biochemistry and Molecular Biology, Birminghan, UK, Jul. 2000.
41.	Hideaki Nagamune, Takatsugu Goto, Robert A. Whiley, Takuya Maeda, Yasuko Inai, Ohki Ohnishi, Kazuto Ohkura, Jeremy M. Hardie and Hiroki Kourai : Structural and Epidemiological Characterization of a Human-Specific Cytolysin, Intermedilysin: A Potentially Significant Virulence Factor in Streptococcus intermedius Infections, 14th Lancefield International Symposium on Streptococci and Streptococcal Diseases, Auckland, NZ, Oct. 1999. (Summary) クローニングされたヒト特異的細胞溶解毒素インターメディリシン(ILY)の遺伝子情報に基づいたPCRによるily遺伝子分布検索の結果，アンギノーサス群連鎖球菌におけるily遺伝子の分布はS.intermedius(SI)のみに限定されていた．しかもヒト口腔内に常在する株に比べて重篤な深部膿瘍からの分離株の場合にはILY分泌量が6-10倍も多いことから，SI感染症にILYが重要な病原因子として働くことを疫学的に示した．
42.	Hideaki Nagamune, Chikako Ohnishi, Ayako Katsuura, Yasunori Taoka, Kenzo Fushitani, Robert A. Whiley, Kikuji Yamashita, Akihiko Tsuji, Yoshiko Matsuda, Hiroki Kourai and Seiichiro Kitamura : Intermedilysin A cytolytic toxin specific for human cells of a Streptococcus intermedius isolated from human liver abscess, XIII Lancefield International Symposium on Streptococci and Streptococcal Diseases; In "Streptococci and The Host"(T. Horaud, A. Bouvet, R. Leclercq, H. de Montclos, M. Sicard, eds.): Advances in Experimental Medicine and Biology, Vol.418, 773-775, Paris, Sep. 1997. (Summary) S. intermedius肝膿瘍分離株から単離されたヒト細胞特異的な細胞溶解毒素インターメディリシン(ILY)の性質を検討した．これまで知られていたチオール基修飾試薬への非感受性やコレステロールへの低感受性に加え，電子顕微鏡観察からILYは直径30-50nmの膜孔を形成し細胞を破壊することが示された．またILYの部分遺伝子構造解析の結果からILYはチオール活性化毒素群と同じ分子祖先から進化したものであることが示唆された．
43.	Hideaki Nagamune, Kazuaki Muramatsu, Masakatsu Higashine, Tetsuya Akamatsu, Yasuhiro Tamai, Yasuo Yonetomi, Akihiko Tsuji, Keisuke Izumi, Takemasa Sakaguchi, Tetsuya Yoshida and Yoshiko Matsuda : Cyclic AMP-inducible procalcitonin processing in thyroidal parafollicular cells is regulated by the Kexin family protease, PC1., International Symposium on Medical Aspects of Protease Inhibitors; In "Medical Aspects of Proteases and Protease Inhibitors": Biomedical and Health Research, Vol.15, 22-33, Amsterdam, May 1997. (Summary) ラット甲状腺においてKexin様プロテアーゼの免疫組織化学的解析を行った．その結果，カルシトニンを作る傍濾胞細胞ではPC1とPC2が強く発現しており，FurinやPC6の発現はわずかで，PACE4は検出できなかった．傍濾胞細胞株TTのウエスタンブロットでもPC1，PC2， Furin，PC6は検出されたが，cAMP刺激による成熟カルシトニン量の上昇と連動して急増する酵素はPC1だけであり，cAMP刺激に応答してカルシトニン量を制御するKexin様プロテアーゼはPC1である事を示した． (Keyword) Calcitonin / Processing / PC1
44.	Tetsuya Akamatsu, 吉田茂, 大黒成夫, Hideaki Nagamune, 長濱正巳, Akihiko Tsuji and Yoshiko Matsuda : Expression of a novel PACE4 isoform, PACE4E, in developmental rat brain, The 12th International Symposium of Federation of Asian and Oceanian Biochemists and Molecular Biologists, Tokushima, Jun. 1996.
45.	Katsuhiko Hirota, Fusao Ota, Sonia Senesi, Hideaki Nagamune, Giovanna Batoni, Giorgio Freer, Terushige Kawata and Komei Fukui : A comparison of various EIA plates for the analysis of polysaccharide antigens and serological identification of oral streptococci, XI Lancefield International Symposium on Streptococci and Streptococcal Diseases; In "New Perspectives on Streptococci and Streptococcal Infections"(G. Orefici, ed.), Zentralblatt für Bakteriologie, Vol.Suppl22, 116-118, Siena, Oct. 1992. (Summary) ミュータンス群連鎖球菌のS. cricetus(血清型a)，S. sobrinus(血清型d)の多糖体抗原及び全菌体に対するMAba-4とMAbh-448の反応性を様々なEIAプレートを用いて解析し比較した．その結果，Corning社のプレートがミュータンス群連鎖球菌の抗原解析や菌種同定に最適であることを示した．
46.	Hideaki Nagamune, Fusao Ota, Sonia Senesi, Giovanna Batoni and Komei Fukui : Monoclonal antibodies specific for a polysaccharide antigenic determinant of Streptococcus mutans (serotypes c, e and f), XI Lancefield International Symposium on Streptococci and Streptococcal Diseases; In "New Perspectives on Streptococci and Streptococcal Infections"(G. Orefici, ed.): Zentralblatt für Bakteriologie, Vol.Suppl22, 223-225, Siena, Oct. 1992. (Summary) 虫歯の最大の原因菌S. mutansに対する単クローン抗体の作成を行い，S. mutans種に特異的な5種の抗体，MAbf77，f89，f93，f125及びf254を得た．その内の4種(MAbf93のみはIgDで残りはIgM)は菌体外の多糖体抗原を認識し，抗原決定基は同じGlcNAcであった．IgG2aのMAbf88のみは多糖体以外の菌体抗原を認識した．これらの抗体は虫歯ハイリスクグループの診断などに利用価値が高いことを示した．
47.	Yoshihiro Fukushima, Junji Takada, Hideaki Nagamune and Mitsuo Yano : Competitive relationship between K+ and (Z)-5-methyl-2-[2-(1-naphthyl)ethenyl]-4-piperidinopyridine for access to the K+-translocating channel of (Na, K)-ATPase, The 6th International Conference on Na,K-ATPase; In "The Sodium Pump: Recent Developments"(J. H. Kaplan, P. DeWeer, eds.), 423-426, Woods Hole, Sep. 1991. (Summary) 疎水性カチオンのAU-1421はNa,K-ATPaseにK+と競合的に結合した．通常，K+はATPでリン酸化された酵素(E2P)の脱リン酸化を促進するが，AU-1421は逆に阻害をしたことからAU-1421はCa++の例と同様にK+結合部位を完全には占有しない可能性を示した．またこのAU-1421はFITCと蛍光エネルギー移動を行える蛍光特性を持つため，ATP結合部位(FITC結合部位)とK+結合部位の距離測定用ツールとして有用であることを示した．
48.	Hideaki Nagamune, Osamu Urayama, Yukichi Hara and Makoto Nakao : Ouabain-sensitive, K+-dependent and K+-independent phosphatase activities in purified pig kidney Na+, K+-ATPase, XI Yamada Conference: In "Energy Transduction in ATPases" (Y. Mukohata, M. F. Morales, S. Fleischer, eds.), 443-446, Kobe, Sep. 1988. (Summary) 豚腎Na,K-ATPaseが触媒するK+非依存性及びK+依存性のウワバイン感受性ホスファターゼ活性を解析したところ，本酵素の基質となるADP，ATP，無機リン酸などはK+非依存性ホスファターゼ反応に競合阻害作用を示し，K+は逆に活性化作用を示して強いK+依存性ホスファターゼ活性を誘導した．また蛍光変化による酵素コンフォメーションの解析から，この両ホスファターゼ反応はE1->E2->E1の循環反応であることを示した．
49.	Osamu Urayama, Hideaki Nagamune and Makoto Nakao : Characterization of monoclonal antibody 50c against pig kidney Na+, K+-ATPase, XI Yamada Conference: In "Energy Transduction in ATPases" (Y. Mukohata, M. F. Morales, S. Fleischer, eds.), 441-442, Kobe, Sep. 1988. (Summary) 豚腎Na,K-ATPaseに対する単クローン抗体mAb50cは酵素の細胞質側に結合し，Na,K-ATPase，Na-ATPase及びK-pNPPase活性を各々5%，0%，20%阻害した．またE1Na型，E2K型及びE2P型酵素への抗体結合数は酵素当たり0.7，0.2，0.4で，蛍光標識酵素に結合した抗体が酵素のE1型->E2型の変化速度を低下させたことから，mAb50cは酵素の構造平衡をE2型->E1型にずらすことを示した．
50.	Yukichi Hara, Osamu Urayama, Kiyoshi Kawakami, Hiroshi Nojima, Hideaki Nagamune, Toshiyuki Kojima, Toshiko Ohta, Kei Nagano and Makoto Nakao : The third type of alpha-subunit of Na,K-ATPase, The 5th International Conference on Na,K-ATPase; In "The Na+, K+-Pump. Part A: Molecular Aspects" (J. C. Skou, J. G. Norby, A. B. Maunsbach, M. Esmann, eds.), 73-78, Fuglso, Apr. 1988. (Summary) Na,K-ATPaseの第三の触媒サブユニット αⅢの構造と分布について解析を行った．本酵素の阻害剤ウワバイン結合部位の構造を，ウワバイン高感受性のヒトなどのサブユニット，低感受性のラット腎型サブユニット及び αⅢ間で比較した結果， αⅢはヒトサブユニットと近い構造をしており，ウワバイン高感受性であることが考えられた．またノーザンブロットの結果， αⅢはラットの腎臓や心臓では検出されず，中枢神経系にのみ発現することを示した．
51.	Osamu Urayama, Hideaki Nagamune, Yukichi Hara and Makoto Nakao : Monoclonal antibodies to pig Na, K-ATPase, The 4th International Conference on Na,K-ATPase; In "The Sodium Pump" (I. Glynn and C. Ellory, eds.), 125-127, Cambridge, Sep. 1985. (Summary) 豚腎Na,K-ATPaseに対する単クローン抗体の調製を試み，二種類のNa,K-ATPaseに特異的な単クローン抗体を得た．一つはmAb38(166KDa，半飽和結合濃度0.6nM)で，他方はmAb50c(162Ka，半飽和結合濃度5.2nM)であった．mAb38は細胞外部分に対し，mAb50cは細胞質側に対して結合した．これらの抗体はNa,K-ATPaseの特異阻害剤ウワバインの結合機構の解析やNa,K-ATPaseの精製に有用であることを示した．
52.	Makoto Nakao, Osamu Urayama, Hideaki Nagamune, Toshiko Nakao, Kanako Sato, Fumiko Nagai and Itsu Kano : Monoclonal antibodies against kidney Na+, K+-ATPase, XI Yamada Conference: In "Perspectives of Biological Energy Transduction" (Y. Mukohata, M. F. Morales, S. Fleischer, eds.), 301-304, Kobe, May 1985. (Summary) Na,K-ATPaseに対する単クローン抗体の性質の解析を行い報告した．mAb50cは豚腎の未変性酵素のE1コンフォメーションを強く認識して結合することを明らかにした．またmAb50cは酵素コンフォメーションをE2->E1にシフトさせることも示した．M45-80抗体については，馬腎の酵素サブユニットに細胞外部側から結合して酵素活性を阻害することを明らかとした．以上のことから，これらの抗体はNa,K-ATPaseの構造や機能解析の有用なツールとなることを示した．

Proceeding of Domestic Conference:

1.	Hiroshi Takashima, Hiromu Matsumura, Yasuhide Ohno, Masao Nagase, Atsushi Tabata and Hideaki Nagamune : 抗体配向修飾SiC上グラフェン膜を用いた蛍光・電気測定による標的検出, 2024年度応用物理・物理系学会中国四国支部合同学術講演会, Vol.Fp-7, Jul. 2024.
2.	Hiromu Matsumura, Yusuke Mori, Hiroshi Takashima, Yasuhide Ohno, Masao Nagase, Tung Anh Hoang, Atsushi Tabata and Hideaki Nagamune : HisタグB-domainを用いたSiC上グラフェンへの抗体配向修飾法, 2024年第71回応用物理学会春季学術講演会, Vol.24a-1BM-7, Mar. 2024.
3.	YAMAMORI Yugo, Hideaki Nagamune, Toshifumi Tomoyasu and Atsushi Tabata : Streptococcus anginosusが産生するStreptolysin Sに対する宿主細胞応答のメカニズム, 第96回日本生化学会大会, Nov. 2023. (Keyword) レンサ球菌 / cytolysin
4.	AOYAMA Rina, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermedius が保有する新規細胞壁アンカータンパク質の機能解析, 第76回日本細菌学会支部総会, Oct. 2023. (Keyword) レンサ球菌 / extracellular matrix
5.	Atsushi Tabata, YOKOHATA Shuto, Kazuto Ohkura, Toshifumi Tomoyasu and Hideaki Nagamune : レンサ球菌が産生するペプチド溶血毒素ストレプトリジンSの血清アルブミンによる活性保護, 第69回トキシンシンポジウム, Sep. 2023. (Keyword) レンサ球菌 / cytolysin
6.	YAMAMORI Yugo, Hideaki Nagamune, Toshifumi Tomoyasu and Atsushi Tabata : Streptococcus anginosus が産生する Streptolysin S に対する宿主細胞応答のメカニズム, 第69回トキシンシンポジウム, Sep. 2023. (Keyword) レンサ球菌 / cytolysin / 宿主細胞応答
7.	OHOKA Keichiroh, Atsushi Tabata, Hisashi Ohkuni, Toshifumi Tomoyasu and Hideaki Nagamune : Induction of cytokine gene expression in human cell line depending on N-terminal domain of Sm-hPAF, 第96回日本細菌学会総会, Mar. 2023. (Keyword) レンサ球菌 / ヒト血小板凝集因子 / cytolysin / 細胞応答
8.	YOKOHATA Shuto, Kazuto Ohkura, Hideaki Nagamune, Toshifumi Tomoyasu and Atsushi Tabata : Human serum albumin stabilizes streptolysin S secreted by streptolysin S-producing streptococci, 第96回日本細菌学会総会, Mar. 2023. (Keyword) ヒト血清アルブミン / 細胞傷害 / cytolysin
9.	OKAMOTO Ryota, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Functional analysis of DnaK chaperone system in Brevibacillus brevis, 第96回日本細菌学会総会, Mar. 2023. (Keyword) Brevibacillus / molecular chaperone / DnaK / グラム陽性菌
10.	Atsushi Tabata, Airi Matsumoto, Ai Fujimoto, Toshifumi Tomoyasu, 髙尾亜由子, Hisashi Ohkuni and Hideaki Nagamune : Molecular characteristics of novel 5-domain type cholesterol-dependent cytolysin, discoidinolysin, 第96回日本細菌学会総会, Mar. 2023. (Keyword) レンサ球菌 / cytolysin / 分子特性
11.	YAMAMORI Yugo, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Mechanism of cellular response to Streptolysin S produced by Streptococcus anginosus, 第96回日本細菌学会総会, Mar. 2023. (Keyword) レンサ球菌 / cytolysin / 細胞応答
12.	TAKESHITA Ryoya, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : 肺がん細胞指向性DDS ツールの作製に向けたリガンド分子の構築, 第146回徳島生物学会, Jan. 2023. (Keyword) 肺がん / DDS ツール / cancer therapy
13.	Yusuke Mori, Hiromu Matsumura, Keita Murayama, Ryoya Takeshita, Anh Hoang Tung, Yasuhide Ohno, Masao Nagase, Atsushi Tabata and Hideaki Nagamune : His-tag 法を用いた SiC 上グラフェンへの抗体配向修飾技術, 第39回「センサ・マイクロマシンと応用システム」シンポジウム, Vol.16P2-P-52, Nov. 2022.
14.	TAKEZAWA Kohji, Atsushi Tabata, Hideaki Nagamune and Toshifumi Tomoyasu : Development and evaluation of a novel micelle-based DDS tool for cancer therapy, 第95回日本生化学会大会, Nov. 2022. (Keyword) ミセル / DDS / cancer therapy
15.	Atsushi Tabata, Airi Matsumoto, Ai Fujimoto, IKEDA Takuya, Toshifumi Tomoyasu, 髙尾亜由子, Hisashi Ohkuni and Hideaki Nagamune : Streptococcus mitisが産生する新規5ドメイン型コレステロール依存性細胞溶解毒素の分子特性と病原性への寄与, 第52回レンサ球菌研究会, Jul. 2022. (Keyword) レンサ球菌 / コレステロール依存性細胞溶解毒素 / 病原性
16.	SHIMIZU Tohya, Toshifumi Tomoyasu, TADA Yuya, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermedius が保有する細胞壁アンカー型エンド-β-N-アセチルグルコサミニダーゼの機能解析, 第52回レンサ球菌研究会, Jul. 2022. (Keyword) レンサ球菌 / 糖鎖 / エンド-β-N-アセチルグルコサミニダーゼ
17.	Atsushi Tabata, YOKOHATA Shuto, Toshifumi Tomoyasu and Hideaki Nagamune : レンサ球菌が産生するペプチド溶血毒素ストレプトリジンSに対するTHP-1の応答反応, 第63回日本生化学会中国四国支部例会, May 2022. (Keyword) レンサ球菌 / 溶血毒素 / 病原性
18.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Functional analysis of a novel cell wall-anchored glycosidase of Streptococcus intermedius, 第95回細菌学会総会, Mar. 2022. (Keyword) Streptococci / グリコシダーゼ / 糖タンパク質 / 糖鎖
19.	ODA Hiroki, Airi Matsumoto, Atsushi Tabata, Toshifumi Tomoyasu, Hisashi Ohkuni and Hideaki Nagamune : Production and molecular characterization of mitilectin homolog derived from S. mitis, 第95回細菌学会総会, Mar. 2022. (Keyword) Streptococci / cytolysin / 宿主細胞定着因子
20.	YOKOHATA Shuto, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Characterization of β-hemolysin produced by Streptococcus phocae, 第95回細菌学会総会, Mar. 2022. (Keyword) Streptococci / cytolysin / ストレプトリジンS / 細胞障害性
21.	KUBO Momoka, Atsushi Tabata, IKEDA Takuya, Toshifumi Tomoyasu, Hisashi Ohkuni and Hideaki Nagamune : Response of human macrophage-like cells to pore-forming toxin produced by S. mitis clinical isolate, 第95回細菌学会総会, Mar. 2022. (Keyword) Streptococci / 川崎病 / cytolysin / inflammatory cytokine
22.	YOKOHATA Shuto, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus phocae が産生するβ溶血因子の作用特性と病原性への寄与, 第74回⽇本細菌学会中国・四国⽀部総会, Oct. 2021. (Keyword) Streptococci / cytolysin / ストレプトリジンS / コレステロール依存性細胞溶解毒素
23.	Kohki Tahara, Masami Itaya, Atsushi Tabata, Hideaki Nagamune, Tetsuro Katayama, Akihiro Furube, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : イミダゾ[1,5-a]ピリジン三座配位子-ホウ素錯体の合成および構造と光物性評価, 2021年光化学討論会, Sep. 2021.
24.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Genetic analysis of the type VII secretion system of Streptococcus intermedius, 第94回日本細菌学会総会, Mar. 2021. (Keyword) レンサ球菌 / Type VII輸送装置 / 病原性
25.	MIKI Haruka, Atsushi Tabata, 菊池賢, LIU HANYU, Toshifumi Tomoyasu and Hideaki Nagamune : Investigation on the cytotoxicity of a cholesterol-dependent cytolysin derived from Gemella bergeri, 第94回日本細菌学会総会, Mar. 2021. (Keyword) レンサ球菌 / cytolysin / 細胞障害性
26.	Atsushi Tabata, Hisashi Ohkuni, Toshifumi Tomoyasu and Hideaki Nagamune : Potential pathogenicity of S. mitis strain Nm-65 based on the complete genomic information, 第94回日本細菌学会総会, Mar. 2021. (Keyword) レンサ球菌 / virulence factor / ゲノム情報
27.	ICHIJOH Ayu, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Purification of cell wall-anchored proteins of Streptococcus intermedius, 第94回日本細菌学会総会, Mar. 2021. (Keyword) レンサ球菌 / extracellular matrix / 細胞壁アンカータンパク質
28.	IKEDA Takuya, Atsushi Tabata, Toshifumi Tomoyasu, 高尾亜由子, Hisashi Ohkuni and Hideaki Nagamune : Dual host cell-binding characteristics of the 5-domain-type CDC with N-terminal extra-domain derived from S. mitis, 第94回日本細菌学会総会, Mar. 2021. (Keyword) レンサ球菌 / cytolysin / N末追加ドメイン
29.	SHIRAI Rina, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Investigation of the cellular response in THP-1 against Streptolysin S produced by S. anginosus, 第94回日本細菌学会総会, Mar. 2021. (Keyword) レンサ球菌 / cytolysin / 免疫担当細胞
30.	Airi Matsumoto, Atsushi Tabata, Kazuto Ohkura, Chihiro Kodama, Hisashi Ohkuni, 高尾亞由子, 菊池賢, Toshifumi Tomoyasu and Hideaki Nagamune : Molecular characteristics of an adhesion molecule containing cholesterol-dependent cytolysin-motif produced by Mitis group streptococci, 第94回日本細菌学会総会, Mar. 2021. (Keyword) レンサ球菌 / 細胞接着分子
31.	Kawasaka Kaito, Koichiro Tsuchiya, Hideaki Nagamune and Akihiro Shirai : フェノール酸とブルーライトを併用した真菌の光不活性化, 日本農芸化学会2021年度仙台大会, Vol.3G01-06, 829, Mar. 2021.
32.	Fumitoshi Yagishita, Shota Kunimi, Keita Hoshi, Masaki Okamoto, Atsushi Tabata, Hideaki Nagamune, Eiji Hase, Keiji Minagawa and Yasushi Imada : 多光子励起可能なイミダゾ[1,2-a]ピリジニウム塩の合成と光細胞傷害性, 日本化学会第101春季年会, Mar. 2021.
33.	Kazuma Kusumoto, Hiroki Takanari, Atsushi Tabata, Hideaki Nagamune, Yukihiro Arakawa, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : D-π-A型ベンゾチアゾール誘導体の合成と光機能性, 2020年日本化学会中国四国支部大会島根大会, Nov. 2020.
34.	Airi Matsumoto, Atsushi Tabata, 高尾亜由子, 菊池賢, Toshifumi Tomoyasu and Hideaki Nagamune : ミチス群レンサ球菌が産生するコレステロール依存性細胞溶解毒素様の細胞接着分子の特性, 第73回日本細菌学会中国・四国支部総会, Oct. 2020.
35.	Kazuma Kusumoto, Yohei Sanagawa, Eiji Hase, Hiroki Takanari, Atsushi Tabata, Hideaki Nagamune, Yukihiro Arakawa, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : 多光子励起を目指したA-D-A型N-へテロアレーン類のデザインと応用, 2020年web光化学討論会, Sep. 2020.
36.	Ryuta Umebayashi, Masaki Okamoto, Eiji Hase, Hiroki Takanari, Atsushi Tabata, Hideaki Nagamune, Yukihiro Arakawa, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : 多光子励起可能な水溶性イミダゾ[1,2-a]ピリジニウム塩の合成とミトコンドリアイメージング, 2020年web光化学討論会, Sep. 2020.
37.	Airi Matsumoto, Atsushi Tabata, Kazuto Ohkura, Chihiro Kodama, Hisashi Ohkuni, 高尾亞由子, 菊池賢, Toshifumi Tomoyasu and Hideaki Nagamune : ミチス群レンサ球菌が産生するコレステロール依存性細胞溶解毒素モチーフを持つ細胞接着分子の特性, 第15回細菌学若手コロッセウム, Aug. 2020. (Keyword) レンサ球菌 / 細胞接着分子
38.	Iori Kishinobu, Daiu Akiyama, Hiroki Nakai, Yoshiaki Taniguchi, Yasuhide Ohno, Hideaki Nagamune and Masao Nagase : Adsorption characteristic of proteins with high pI to a graphene film on SiC substrates, 2020年度応用物理・物理系学会中国四国支部合同学術講演会, Vol.Ga-2, Aug. 2020.
39.	Atsushi Tabata, Kazuto Ohkura, Hisashi Ohkuni, Miho Kobayashi, Airi Matsumoto, Toshifumi Tomoyasu and Hideaki Nagamune : The structural and functional diversity of cholesterol-dependent cytolysin produced from Streptococci, 第20回日本蛋白質科学会年会, Jul. 2020.
40.	Airi Matsumoto, Atsushi Tabata, 高尾亞由子, 菊池賢, Toshifumi Tomoyasu and Hideaki Nagamune : Cholesterol-dependent cytolysin-like adhesion protein of Mitis group streptococci, 第20回日本蛋白質科学会年会, Jul. 2020.
41.	Fumitoshi Yagishita, Junichi Tanigawa, Masaki Okamoto, Eiji Hase, Hiroki Takanari, Atsushi Tabata, Hideaki Nagamune, Takeo Minamikawa, Takeshi Yasui, Yasushi Imada and Yasuhiko Kawamura : Synthesis of Imidazopyridinium Salts Having Potential Application as Image-guided Photodynamic Therapy Agents, The 100th CSJ Annual Meeting, Mar. 2020.
42.	Toshifumi Tomoyasu, Mari Deguchi, Atsushi Tabata, 髙尾亞由子, 前田伸子 and Hideaki Nagamune : Streptococcus intermediusのヒアルロニダーゼ発現制御機構についての解析, 第93回日本細菌学会総会, Feb. 2020. (Keyword) ヒアルロニダーゼ / gene expression / Streptococci
43.	Airi Matsumoto, Atsushi Tabata, 髙尾亞由子, 菊池賢, Toshifumi Tomoyasu, 前田伸子 and Hideaki Nagamune : ミチス群レンサ球菌が産生するコレステロール依存性細胞溶解毒素様の細胞接着分子, 第93回日本細菌学会総会, Feb. 2020. (Keyword) 細胞接着因子 / Streptococci / cytolysin
44.	Chihiro Kodama, Atsushi Tabata, Toshifumi Tomoyasu, 髙尾亞由子, 前田伸子 and Hideaki Nagamune : S. infantisが産生するコレステロール依存性細胞溶解毒素infantilysinの特性解析, 第93回日本細菌学会総会, Feb. 2020. (Keyword) cytolysin / cholesterol / Streptococci
45.	Fumitoshi Yagishita, Masaki Okamoto, Hiroki Takanari, Eiji Hase, Atsushi Tabata, Hideaki Nagamune, Yasushi Imada, Takeshi Yasui and Yasuhiko Kawamura : ミトコンドリアイメージングを可能とするイミダゾ[1,2-a]ピリジニウム塩の合成, 2019年日本化学会中国四国支部大会徳島大会, Nov. 2019.
46.	Fumitoshi Yagishita, Junichi Tanigawa, Chiho Nii, Atsushi Tabata, Hideaki Nagamune, Hiroki Takanari, Yasushi Imada and Yasuhiko Kawamura : 光線力学療法への応用を狙いとしたイミダゾ[1,5-a]ピリジニウム塩の合成, 2019年日本化学会中国四国支部大会徳島大会, Nov. 2019.
47.	Akihiro Shirai, 國見明加 and Hideaki Nagamune : 紫外線-Aとフェルラ酸の併用殺菌機構の解析とポストハーベスト殺菌への応用, 日本防菌防黴学会第46回年次大会要旨集, No.2P-AA36, 201, Sep. 2019.
48.	Airi Matsumoto, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : S. pseudopneumoniaeが産生するコレステロール依存性細胞溶解毒素様の細胞接着分子, 第92回日本生化学会大会, Sep. 2019. (Keyword) 細胞接着 / レンサ球菌 / virulence factor
49.	Toshifumi Tomoyasu, Akiko Tominaga, Mari Deguchi, Eri Arimoto, Atsushi Tabata, 高尾亞由子, 前田伸子 and Hideaki Nagamune : Streptococcus intermedius が分泌する病原因子の特性解析, 第66回トキシンシンポジウム, Sep. 2019. (Keyword) cytolysin / ヒアルロニダーゼ / レンサ球菌
50.	Fumitoshi Yagishita, Ryuta Umebayashi, Masaki Okamoto, Atsushi Tabata, Hideaki Nagamune, Hiroki Takanari, Yasushi Imada and Yasuhiko Kawamura : D-π-A 型イミダゾ [1,2-a] ピリジニウム塩の合成とミトコンドリアイメージングへの応用, 2019年光化学討論会, Sep. 2019.
51.	Chihiro Kodama, QING TANG, Atsushi Tabata, 髙尾亞由子, Toshifumi Tomoyasu, 前田伸子 and Hideaki Nagamune : Streptococcus infantisが産生する新規コレステロール依存性細胞溶解毒素infantilysinの作用特性の解明, 第13回細菌学若手コロッセウム, Aug. 2019. (Keyword) Streptococci / cytolysin
52.	Ayu Ichijoh, Toshifumi Tomoyasu, Eri Arimoto, Mari Deguchi, Atsushi Tabata, 高尾亞由子, 前田伸子 and Hideaki Nagamune : Streptococcus intermediusのヒアルロニダーゼ発現制御機構の解析, 第13回細菌学若手コロッセウム, Aug. 2019. (Keyword) 連鎖球菌 / ヒアルロン酸分解酵素 / extracellular matrix
53.	Rina Shirai, Miho Kobayashi, Atsushi Tabata, Hisashi Ohkuni, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus mitisが産生する非典型CDCに対する細胞の応答反応, 第13回細菌学若手コロッセウム, Aug. 2019. (Keyword) Streptococci / cytolysin
54.	Airi Matsumoto, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus pseudopneumoniaeが産生するコレステロール依存性細胞溶解毒素様の細胞接着分子, 第13回細菌学若手コロッセウム, Aug. 2019. (Keyword) Streptococci / 細胞接着分子 / cytolysin
55.	Airi Matsumoto, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus pseudopneumoniaeが産生するコレステロール依存性細胞溶解毒素様の細胞接着分子, 第51回レンサ球菌研究会, Jun. 2019. (Keyword) Streptococcus / cytolysin / 細胞接着分子
56.	Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus anginosus subsp. anginosus由来溶原性ファージの遺伝子的特性, 第51回レンサ球菌研究会, Jun. 2019. (Keyword) Streptococcus / bacteriophage
57.	Toshifumi Tomoyasu, Mari Deguchi, 高尾亞由子, Atsushi Tabata, 前田伸子 and Hideaki Nagamune : Streptococcus intermediusが保有する分泌型グリコシダーゼの局在性, 第92回日本細菌学会総会, Apr. 2019. (Keyword) Streptococcus / グリコシダーゼ / 局在性
58.	Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus anginosus由来溶原性ファージの特性, 第92回日本細菌学会総会, Apr. 2019. (Keyword) Streptococcus / bacteriophage
59.	Airi Matsumoto, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : S. pseudopneumoniaeが産生するコレステロール依存性細胞溶解毒素様分子の特性解析, 第92回日本細菌学会総会, Apr. 2019. (Keyword) Streptococcus / cytolysin / evolution
60.	Chihiro Kodama, QING TANG, Atsushi Tabata, 高尾亞由子, Toshifumi Tomoyasu, 前田伸子 and Hideaki Nagamune : Streptococcus infantisが産生するinfantilysinの分子特性, 第92回日本細菌学会総会, Apr. 2019. (Keyword) Streptococcus / cytolysin
61.	Miho Kobayashi, Atsushi Tabata, Hisashi Ohkuni, 高尾亞由子, Toshifumi Tomoyasu, 前田伸子 and Hideaki Nagamune : Streptococcus mitisが産生する5ドメイン型CDCに対するヒト好中球様細胞の応答反応, 第92回日本細菌学会総会, Apr. 2019. (Keyword) Streptococcus / cytolysin
62.	Fumitoshi Yagishita, Junichi Tanigawa, Chiho Nii, Atsushi Tabata, Hideaki Nagamune, Yasushi Imada and Yasuhiko Kawamura : 水溶性N-へテロアレーンを用いたHeLa細胞の蛍光イメージングと光細胞毒性, 日本化学会第99春季年会, Mar. 2019.
63.	Yurina Nishihira, Atsushi Tabata, Toshifumi Tomoyasu, Kazuto Ohkura and Hideaki Nagamune : 薬剤キャリアのミセルが示す癌細胞障害性, 第22回バイオ治療法研究会学術集会, Dec. 2018. (Keyword) drug delivery system / cancer therapy
64.	Fumitoshi Yagishita, Junichi Tanigawa, Chiho Nii, Atsushi Tabata, Hideaki Nagamune, Yasushi Imada and Yasuhiko Kawamura : 光照射をトリガーとした水溶性N-ヘテロアレーンによるDNA損傷, 2018年日本化学会中国四国支部大会, Nov. 2018.
65.	Haruka Kunimi, Hideaki Nagamune and Akihiro Shirai : 酵母に対する紫外線-Aとフェルラ酸の併用殺菌機構の解析, 日本防菌防黴学会第45回年次大会要旨集, No.2P-Cp70, 329, Nov. 2018.
66.	Mari Deguchi, Toshifumi Tomoyasu, 高尾亞由子, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermediusが保有するグリコシダーゼの局在性についての解析, 第71回日本細菌学会中国・四国支部総会, Oct. 2018. (Keyword) Streptococcus / グリコシダーゼ / 局在性
67.	Haruka Kunimi, Hideaki Nagamune and Akihiro Shirai : 紫外線-Aとフェルラ酸を利用したポストハーベスト殺菌手法の検討, 第39回日本食品微生物学会学術総会講演要旨集, No.P18, 108, Sep. 2018.
68.	山田拓矢, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Response of host human cells against SLS-homolog derived from S. anginosus subsp. anginosus, 第91回日本生化学会大会, Sep. 2018.
69.	Nobutake Tamai, 森光達, Masaki GOTO, Hideaki Nagamune and Hitoshi Matsuki : リン脂質-コレステロール混合二分子膜に対するコレステロール依存性細胞溶解毒素の結合活性, 第69回コロイドおよび界面化学討論会, Sep. 2018.
70.	Nobutake Tamai, 森光達, Masaki GOTO, Hideaki Nagamune and Hitoshi Matsuki : モデル膜を用いたコレステロール依存性細胞溶解毒素の膜結合活性評価, 第56回生物物理学会年会, Sep. 2018.
71.	Junichi Tanigawa, Fumitoshi Yagishita, Chiho Nii, Atsushi Tabata, Hideaki Nagamune, Yasushi Imada and Yasuhiko Kawamura : 水溶性N-ヘテロアレーンの蛍光バイオイメージングへの応用とDNA損傷, 2018年光化学討論会, Sep. 2018.
72.	Junichi Tanigawa, Fumitoshi Yagishita, Chiho Nii, Atsushi Tabata, Hideaki Nagamune, Yasushi Imada and Yasuhiko Kawamura : 蛍光発光性N-ヘテロアレーンのバイオイメージングへの応用とDNA損傷, 第48回複素環化学討論会, Sep. 2018.
73.	Fumitoshi Yagishita, Shota Unesaki, Yoshihiko Tezuka, Atsushi Tabata, Hideaki Nagamune and Yasuhiko Kawamura : スチリル基を有するイミダゾ[1,5-a]ピリジン二量体の金属イオン存在下における発光挙動, 第48回複素環化学討論会, Sep. 2018.
74.	Yohhei Sanagawa, Fumitoshi Yagishita, Atsushi Tabata, Hideaki Nagamune and Yasuhiko Kawamura : A-π-D-π-A システムに基づく新規対称発光分子のデザインと合成，及び光物性, 第48回複素環化学討論会, Sep. 2018.
75.	児玉千紘, 唐卿, Atsushi Tabata, 髙尾亞由子, Toshifumi Tomoyasu, 前田伸子 and Hideaki Nagamune : Streptococcus infantisが産生するInfantilysinの作用特性を明らかにする, 第12回細菌学若手コロッセウム in OKAYAMA, Aug. 2018.
76.	藤本あい, Atsushi Tabata, 髙尾亞由子, Hisashi Ohkuni, Toshifumi Tomoyasu, 前田伸子 and Hideaki Nagamune : Streptococcus mitisが産生する新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性の多様性に関する研究, 第12回細菌学若手コロッセウム in OKAYAMA, Aug. 2018.
77.	松本愛理, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus pseudopneumoniaeが保有するコレステロール依存性細胞溶解毒素様分子の解析, 第12回細菌学若手コロッセウム in OKAYAMA, Aug. 2018.
78.	Atsushi Tabata, 松本愛理, 藤本あい, 児玉千紘, Toshifumi Tomoyasu and Hideaki Nagamune : 咽頭口腔レンサ球菌が産生する溶血毒素とその多様性, 第12回細菌学若手コロッセウム in OKAYAMA, Aug. 2018.
79.	Airi Matsumoto, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : S. pseudopneumoniaeが保有するコレステロール依存性細胞溶解毒素様分子の解析, 第65回トキシンシンポジウム, Jul. 2018. (Keyword) Streptococcus / cytolysin
80.	Ai Fujimoto, Atsushi Tabata, 髙尾亞由子, Hisashi Ohkuni, Toshifumi Tomoyasu, 前田伸子 and Hideaki Nagamune : Streptococcus mitisが産生する新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性, 第65回トキシンシンポジウム, Jul. 2018. (Keyword) Streptococcus / cytolysin / cholesterol
81.	Akiko Tominaga, Toshifumi Tomoyasu, Tatsuma Kusaka, Atsushi Tabata, 髙尾亞由子, Hisashi Ohkuni, 前田伸子 and Hideaki Nagamune : グループ3に属するコレステロール依存性細胞溶解毒素のCD59認識性の比較, 第65回トキシンシンポジウム, Jul. 2018. (Keyword) Streptococcus / cytolysin / cholesterol
82.	Atsushi Tabata, Seiya Otsuka, Deutsch Douglas, 菊池賢, Fischetti A. Vincent, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus anginosus subsp. anginosusが保有するプラスミドとその特徴, 第50回レンサ球菌研究会, Jun. 2018. (Keyword) Streptococcus / プラスミド
83.	Toshifumi Tomoyasu, Masaki Matoba, Shingo Kusaka, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermediusのグリコシダーゼとインターメディリシン産生との関連について, 第50回レンサ球菌研究会, Jun. 2018. (Keyword) Streptococcus / virulence factor / cytolysin / sugar
84.	Nobutake Tamai, 森光達, Masaki GOTO, Hideaki Nagamune and Hitoshi Matsuki : コレステロール依存性細胞溶解毒素の脂質膜に対する結合活性, 日本膜学会第40年会, May 2018.
85.	立松洋平, Atsushi Tabata, Hideaki Nagamune and 大倉一人 : 非ステロイド性抗炎症薬セレコキシブの細胞膜障害メカニズムの解析, 第91回日本細菌学会総会, Mar. 2018.
86.	Haruka Hino, Tatsuya Okahata, Atsushi Tabata, 髙尾亞由子, 大國寿士, 小椋義俊, Toshifumi Tomoyasu, 林哲也, 前田伸子 and Hideaki Nagamune : Streptococcus mitisにおけるコレステロール依存性細胞溶解毒素の遺伝子分布パターンと細胞障害性との関連性, 第91回日本細菌学会総会, Mar. 2018.
87.	Risako Araki, Atsushi Tabata, 村上漱, 髙尾亞由子, Hisashi Ohkuni, 小椋義俊, Toshifumi Tomoyasu, 林哲也, 前田伸子 and Hideaki Nagamune : タンデム型末追加ドメインを持つコレステロール依存性細胞溶解毒素の特性解析, 第91回日本細菌学会総会, Mar. 2018. (Keyword) pathogenic microorganism / bacterial toxins
88.	Takuya Yamada, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Transcriptional response of host cells induced by SLS-producing S. anginosus subsp. anginosus, 第91回日本細菌学会総会, Mar. 2018. (Keyword) pathogenic microorganism / bacterial toxins
89.	高尾亞由子, Toshifumi Tomoyasu, Atsushi Tabata, Hideaki Nagamune and 前田伸子 : グリコシダーゼ活性が Streptococcus intermedius の実験的バイオフィルム量に及ぼす影響, 第91回日本細菌学会総会, Mar. 2018. (Keyword) pathogenic microorganism / bacterial toxins
90.	Yayoi Iwasa, Asuka Fukutomi, Atsushi Tabata, 村上漱, Toshifumi Tomoyasu and Hideaki Nagamune : Multiplex-PCR による高病原性 S. mitis 検出系の実用化に向けた検討, 第91回日本細菌学会総会, Mar. 2018. (Keyword) pathogenic microorganism / bacterial toxins
91.	Atsushi Tabata, Seiya Otsuka, 菊池賢, Toshifumi Tomoyasu and Hideaki Nagamune : S. anginosus subsp. anginosusにおけるプラスミドの保有状況とそれらの特性, 第91回日本細菌学会総会, Mar. 2018. (Keyword) pathogenic microorganism / virulence factor
92.	Toshifumi Tomoyasu, Akiko Tominaga, Atsushi Tabata, 髙尾亞由子, Hisashi Ohkuni, 前田伸子 and Hideaki Nagamune : DTT処理赤血球を用いたコレステロール依存性細胞溶解毒素の CD59認識性の比較, 第91回日本細菌学会総会, Mar. 2018. (Keyword) pathogenic microorganism / bacterial toxins
93.	Ai Fujimoto, Atsushi Tabata, 髙尾亞由子, Hisashi Ohkuni, 前田伸子, Toshifumi Tomoyasu and Hideaki Nagamune : S. mitis由来の新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性の多様性に関する研究, 第91回日本細菌学会総会, Mar. 2018. (Keyword) pathogenic microorganism / bacterial toxins
94.	谷脇竜弥, 杉田知世, Keiji Murakami, Takashi Amoh, Atsushi Tabata, Yoshiko Yamamura, Tetsuya Tamatani, Hideaki Nagamune, Youji Miyamoto and Hideki Fujii : 幼児口腔内創部より検出された多剤耐性レンサ球菌について, 四国歯学会第52回例会, Mar. 2018.
95.	Fumitoshi Yagishita, Chiho Nii, Yoshihiko Tezuka, Atsushi Tabata, Hideaki Nagamune, Shoko Ueta and Yasuhiko Kawamura : Synthesis and Evaluation of Photophysical Properties of Dimeric Imidazo[1,5-a]pyridinium Salts, 日本化学会第98春季年会, Mar. 2018.
96.	Akiko Tominaga, Toshifumi Tomoyasu, Tatsuma Kusaka, Atsushi Tabata, 髙尾亞由子, Hisashi Ohkuni, 前田伸子 and Hideaki Nagamune : グループ3に属するコレステロール依存性細胞溶解毒素のCD59認識性の比較, 2017年度生命科学系学会合同年次大会, Dec. 2017. (Keyword) pathogenic microorganism / bacterial toxins
97.	Shingo Kusaka, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : シアリダーゼ阻害剤がStreptococcus intermediusのシアリダーゼ活性や溶血活性に及ぼす効果, 2017年度生命科学系学会合同年次大会, Dec. 2017. (Keyword) pathogenic microorganism / virulence factor
98.	Kazuki Hatta, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : ナノ粒子表面加工システムの構築に用いる細菌由来ペプチド転移酵素Sortase A組換え体の作製とその特性, 2017年度生命科学系学会合同年次大会, Dec. 2017. (Keyword) cancer therapy
99.	Chiho Okada, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : DDS用エフェクター分子としての細菌毒素組換え体の効率的な調製システムの構築, 2017年度生命科学系学会合同年次大会, Dec. 2017. (Keyword) cancer therapy
100.	Qing Tang, Masayoshi Onitsuka, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : 癌治療用新規DDSツール作製に用いるリガンド分子の調製とその機能評価, 第21回バイオ治療法研究会, Dec. 2017. (Keyword) cancer therapy
101.	Ai Fujimoto, Atsushi Tabata, 髙尾亞由子, Hisashi Ohkuni, 前田伸子, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus mitis由来新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性を規定する構造領域の探索, 第70回日本細菌学会中国・四国支部総会, Oct. 2017. (Keyword) pathogenic microorganism / bacterial toxins
102.	Toshifumi Tomoyasu, Shingo Kusaka, Shinya Chiba, Takahiro Yamasaki, Atsushi Tabata and Hideaki Nagamune : ヒト血漿によるStreptococcus intermediusの病原性発現抑制機構の解析, 第70回日本細菌学会中国・四国支部総会, Oct. 2017. (Keyword) pathogenic microorganism / virulence factor
103.	Haruka Kunimi, Hideaki Nagamune and Akihiro Shirai : ポストハーベスト殺菌に資する紫外線-Aとフェルラ酸の併用手法の検討, 第38回日本食品微生物学会学術総会講演要旨集, No.P38, 130, Oct. 2017.
104.	Haruka Kunimi, Hideaki Nagamune and Akihiro Shirai : 紫外線-Aとフェルラ酸の併用殺菌法を用いた酵母の増殖抑制の検討, 日本防菌防黴学会第44回年次大会要旨集, No.2P-Aa52, 189, Sep. 2017.
105.	Yosuke Bando, Hideaki Nagamune and Akihiro Shirai : 紫外線-A反応分子であるフェルラ酸を用いたフェノール酸誘導体の合成とその殺菌特性, 日本防菌防黴学会第44回年次大会要旨集, No.2P-Aa58, 195, Sep. 2017.
106.	Airi Matsumoto, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : S. mitis由来5ドメイン型コレステロール依存性細胞溶解毒素のN末追加ドメインが示す細胞障害作用, 第11回細菌学若手コロッセウム, Aug. 2017. (Keyword) pathogenic microorganism / bacterial toxins
107.	Ai Fujimoto, Atsushi Tabata, 髙尾亞由子, Hisashi Ohkuni, 前田伸子, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus mitis が産生する新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性の多様性に関する研究, 第11回細菌学若手コロッセウム, Aug. 2017. (Keyword) pathogenic microorganism / bacterial toxins
108.	Toshifumi Tomoyasu, Atsushi Tabata, Shinya Chiba, Takahiro Yamasaki, Nozomi Takeda, Masaaki Tamaoka, Kazuto Ohkura and Hideaki Nagamune : 糖によるStreptococcus intermediusの病原性制御機構, 第64回トキシンシンポジウム, Jul. 2017. (Keyword) pathogenic microorganism / virulence factor
109.	Haruka Kunimi, Hideaki Nagamune and Akihiro Shirai : 酵母に対する紫外線-Aとフェルラ酸の併用殺菌力とその殺菌機構, 日本農芸化学会中四国支部第48回講演会, No.A-2, 21, Jun. 2017.
110.	Tatsuya Okahata, Atsushi Tabata, 小椋義俊, 林哲也, 高尾亞由子, Hisashi Ohkuni, Toshifumi Tomoyasu, 前田伸子 and Hideaki Nagamune : Streptococcus mitis におけるコレステロール依存性細胞溶解毒素遺伝子の周辺遺伝子配座の多様性とその特徴, 第58回日本生化学会中国・四国支部例会, May 2017. (Keyword) bacterial toxins / gene
111.	Seiya Ohtsuka, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus anginosus subsp. anginosusが保有する新規Extra-chromosomal DNAの分子特性, 第90回日本細菌学会総会, Mar. 2017. (Keyword) Streptococcus / プラスミド / 可動遺伝子
112.	Masaki Matoba, Toshifumi Tomoyasu, Ayuko Takao, Atsushi Tabata, nobuko Maeda and Hideaki Nagamune : Streptococcus intermediusの高病原性株スクリーニングシステムの開発, 第90回日本細菌学会総会, Mar. 2017. (Keyword) Streptococcus / virulence factor / cytolysin
113.	Tatsuya Okahata, Syu Murakami, Atsushi Tabata, Yoshitoshi Ogura, Tetsuya Hayashi, Ayuko Takao, Hisashi Ohkuni, Toshifumi Tomoyasu, Nobuko Maeda and Hideaki Nagamune : Streptococcus mitisが保有するコレステロール依存性細胞溶解毒素遺伝子周辺における遺伝子配座の多様性, 第90回日本細菌学会総会, Mar. 2017. (Keyword) Streptococcus / cytolysin / genome
114.	Airi Matsumoto, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : S. mitis由来5ドメイン型コレステロール依存性細胞溶解毒素のN末追加ドメインが示す細胞障害作用, 第90回日本細菌学会総会, Mar. 2017. (Keyword) Streptococcus / cytolysin
115.	Tatsuma Kusaka, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : ウマ赤血球に存在するExD-CDCレセプターの探索, 第3回日本生物工学会西日本支部講演会, Dec. 2016. (Keyword) Streptococcus / cytolysin / CD59
116.	Ai Fujimoto, Atsushi Tabata, Ayuko Takao, Hisashi Ohkuni, Nobuko Maeda, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus mitisが産生する新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性の多様性, 第3回日本生物工学会西日本支部講演会, Dec. 2016. (Keyword) Streptococcus / cytolysin
117.	Takuya Saigoh, Atsushi Tabata, Ayuko Takao, Hisashi Ohkuni, Nobuko Maeda, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus mitisにおける効果的な形質転換法の検討, 第3回日本生物工学会西日本支部講演会, Dec. 2016. (Keyword) Streptococcus / 形質転換法 / プラスミド / DNA断片
118.	Atsushi Tabata, Kazuto Ohkura, Youhei Tatematsu, Toshifumi Tomoyasu and Hideaki Nagamune : ヒト特異的な細胞障害性を示す細菌毒素に対する糖の阻害作用, 第20回バイオ治療法研究会, Dec. 2016. (Keyword) Streptococcus / cytolysin / sugar
119.	Shingo Kusaka, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermedius が産生するシアリダーゼの発現制御機構の解析, 第69回日本細菌学会中国・四国支部総会, Oct. 2016. (Keyword) Streptococcus / cytolysin / sugar
120.	Ikumi Tamura, Atsushi Tabata, Syu Murakami, Ayuko Takao, Toshifumi Tomoyasu, Nobuko Maeda and Hideaki Nagamune : Streptococcus infantis が産生する新規コレステロール依存性細胞溶解毒素Infantilysin, 第63回トキシンシンポジウム, Jul. 2016. (Keyword) Streptococcus / cytolysin
121.	Toshifumi Tomoyasu, Shinya Chiba, Takahiro Yamasaki, Shingo Kusaka, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermedius の血液成分による病原性亢進とヒト血液成分によるその防御, 第48回レンサ球菌研究会, Jul. 2016. (Keyword) Streptococcus / cytolysin / sugar / antibody
122.	Ayuko Takao, Hideaki Nagamune, Toshifumi Tomoyasu and Nobuko Maeda : Effects of quorum sensing peptides and environmental factors on Streptococcus intermedius, 第89回日本細菌学会総会, Mar. 2016. (Keyword) Streptococcus / quorum sensing / cytolysin
123.	Ikumi Tamura, Atsushi Tabata, Shu Murakami, Ayuko Takao, Hisashi Ohkuni, Toshifumi Tomoyasu, Nobuko Maeda and Hideaki Nagamune : Cytotoxic properties of a novel cholesterol-dependent cytolysin from Streptococcus infantis, 第89回日本細菌学会総会, Mar. 2016. (Keyword) Streptococcus / cytolysin / function
124.	Hiromi Ohtani, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Streptolysin S homolog-dependent cytotoxicity of betahemolytic S. anginosus subsp. anginosus, 第89回日本細菌学会総会, Mar. 2016. (Keyword) Streptococcus / cytolysin / function / gene expression
125.	Toshifumi Tomoyasu, Shinya Kusaka, Masaki Matoba, Yukihiro Kaneko, Atsushi Tabata and Hideaki Nagamune : Wide upstream region of ily is involved in its repression in Streptococcus intermedius, 第89回日本細菌学会総会, Mar. 2016. (Keyword) Streptococcus / cytolysin / gene expression
126.	Shinya Chiba, Toshifumi Tomoyasu, Takahiro Yamasaki, Shingo Kusaka, Atsushi Tabata and Hideaki Nagamune : Positive and negative control of ily expression by blood components, 第89回日本細菌学会総会, Mar. 2016. (Keyword) Streptococcus / cytolysin / blood / sugar
127.	Qing Tang, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : A trial for development of effective drug delivery system using small targeting module against CEA-positive cancer, Joint Meeting of the 38th Annual Meeting of the Molecular Biology Society of Japan and the 88th Annual Meeting of the Japanese Biochemical Society, Dec. 2015. (Keyword) drug delivery system / antibody
128.	Ryosuke Tanatsugu, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Functional analysis of a gram-negative characteristic segment of DnaK using Streptococcus intermedius DnaK, 第38回日本分子生物学会年会, Dec. 2015. (Keyword) Streptococcus / molecular chaperone / protein
129.	Shinya Kusaka, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Investigation of transcriptional regulation elements in ily promoter region of Streptococcus intermedius, 第38回日本分子生物学会年会, Dec. 2015. (Keyword) Streptococcus / cytolysin / gene expression
130.	Shinya Chiba, Toshifumi Tomoyasu, Takahiro Yamasaki, Atsushi Tabata and Hideaki Nagamune : 血液成分によるインターメディリシン発現調節機構の解析, 第68回日本細菌学会中国・四国支部総会, Oct. 2015. (Keyword) Streptococcus / cytolysin / sugar / blood
131.	Ikumi Tamura, Atsushi Tabata, Shu Murakami, Ayuko Takao, Hisashi Ohkuni, Toshifumi Tomoyasu, Nobuko Maeda and Hideaki Nagamune : Streptococcus infantis由来の新規コレステロール依存性細胞溶解毒素の作用特性, 第68回日本細菌学会中国・四国支部総会, Oct. 2015. (Keyword) Streptococcus / cytolysin / virulence factor
132.	Toshifumi Tomoyasu, Shinya Chiba, Takahiro Yamasaki, 甲斐瑛之, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermediusのグリコシダーゼによるインターメディリシン発現調節機構の解析, 第56回日本生化学会中国四国支部例会, May 2015. (Keyword) Streptococci / enzyme / sugar / cytolysin / gene expression
133.	Kentaro Maya, Atsushi Tabata, Kazuto Ohkura, 菊池賢, Toshifumi Tomoyasu and Hideaki Nagamune : S. anginosus subsp. whileyi およびS. constellatus subsp. viborgensis が保有するβ溶血因子の特性解析, 第88回日本細菌学会総会, Mar. 2015. (Keyword) Streptococci / cytolysin / function
134.	Atsushi Morishita, Atsushi Tabata, Shu Murakami, 高尾亞由子, Hisashi Ohkuni, 小椋義俊, 林哲也, Toshifumi Tomoyasu, 前田伸子 and Hideaki Nagamune : S. mitis におけるコレステロール依存性細胞溶解毒素遺伝子の分布とβ-ラクタム系薬剤耐性の関連性, 第88回日本細菌学会総会, Mar. 2015. (Keyword) Streptococci / antibiotics / cytolysin
135.	Takahiro Yamasaki, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : S. intermedius が保有するグリコシダーゼがインターメディリシン発現制御に果たす役割についての解析, 第88回日本細菌学会総会, Mar. 2015. (Keyword) Streptococci / gene expression / enzyme / sugar
136.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermedius が保有するシアリダーゼの精製とその性状の解析, 第88回日本細菌学会総会, Mar. 2015. (Keyword) Streptococci / protein / sugar
137.	Hajime Yoshida, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Inerolysin (INY) の pH 依存的な膜孔形成活性と立体構造変化, 第133回徳島生物学会総会, Dec. 2014. (Keyword) pathogenic microorganism / Streptococci / cytolysin / pH
138.	Yukimasa Okubo, Natsuki Anyohji, Atsushi Tabata, 北條恵子, Toshifumi Tomoyasu, 立松洋平, Kazuto Ohkura and Hideaki Nagamune : ドラッグデリバリーシステム創成を目指したSortase Aによるペプチド修飾化リポソーム作製技術の開発, 第18回バイオ治療法研究会, Dec. 2014. (Keyword) drug delivery system / Sortase A / liposomes
139.	Yukimasa Okubo, Natsuki Anyohji, Atsushi Tabata, 北條恵子, Toshifumi Tomoyasu and Hideaki Nagamune : 新規ドラッグデリバリーシステムツール作製法としてのペプチド修飾化リポソームの作製, 第87回日本生化学会大会, Oct. 2014. (Keyword) drug delivery system / liposomes / ガン治療
140.	Masaaki Tamaoka, Nozomi Takeda, Atsushi Tabata, Kazuto Ohkura, Toshifumi Tomoyasu, Hisashi Ohkuni and Hideaki Nagamune : コレステロール依存性細胞溶解毒素の膜孔形成活性に対する糖の抑制効果, 第87回日本生化学会大会, Oct. 2014. (Keyword) pathogenic microorganism / Streptococcus / virulence factor / sugar / cytolysin
141.	Natsuki Anyohji, Atsushi Tabata, Yukimasa Okubo, Toshifumi Tomoyasu and Hideaki Nagamune : 細菌由来ペプチド転移酵素Sortase Aの由来菌種依存的な基質認識特性, 第87回日本生化学会大会, Oct. 2014. (Keyword) グラム陽性菌 / ブドウ球菌 / Streptococcus / Sortase A / 基質特異性
142.	Atsushi Morishita, Atsushi Tabata, Shu Murakami, 高尾亞由子, 勝部康弘, Hisashi Ohkuni, Toshifumi Tomoyasu, 前田伸子 and Hideaki Nagamune : Streptococcus mitisにおける膜孔形成毒素遺伝子の保有とβ-ラクタム薬耐性の関連性, 第67回日本細菌学会中国四国支部総会, Oct. 2014. (Keyword) pathogenic microorganism / Streptococcus / virulence factor / 溶血因子 / 薬剤耐性
143.	Takahiro Yamasaki, Toshifumi Tomoyasu, Hidenori Imaki, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermediusの病原性とグリコシダーゼとの関連性, 第67回日本細菌学会中国四国支部総会, Oct. 2014. (Keyword) pathogenic microorganism / Streptococcus / sugar / グリコシダーゼ
144.	Toshifumi Tomoyasu, 今木英統, Chiharu Taue, Naoki Yamamoto, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermedius が保有するグリコシダーゼの病原性に果たす役割について, 第61 回トキシンシンポジウム ∼鳴門渦潮カンファレンス∼, Sep. 2014. (Keyword) Streptococcus / pathogenic microorganism / sugar / グリコシダーゼ
145.	Atsushi Tabata, Kentaro Maya, 大谷浩美, Yuji Sato, Kota Nakano, Kazuto Ohkura, 菊池賢, Toshifumi Tomoyasu and Hideaki Nagamune : β溶血性アンギノーサス群レンサ球菌が保有するストレプトリジンSホモログの多様性, 第46回レンサ球菌研究会, Jun. 2014. (Keyword) Streptococcus / pathogenic microorganism / virulence factor / 溶血因子
146.	Atsushi Tabata, Nozomi Takeda, Masaaki Tamaoka, Toshifumi Tomoyasu, 高尾亞由子, 前田伸子, Hisashi Ohkuni and Hideaki Nagamune : コレステロール依存性細胞溶解毒素の膜孔形成活性に及ぼす糖の影響, 第55回日本生化学会中国・四国支部例会, Jun. 2014. (Keyword) Streptococcus / pathogenic microorganism / virulence factor / sugar
147.	Toshifumi Tomoyasu, 今木英統, Chiharu Taue, Naoki Yamamoto, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermediusが保有する多基質酵素``MsgA''の性状解析, 第55回日本生化学会中国・四国支部例会, Jun. 2014. (Keyword) Streptococcus / pathogenic microorganism / virulence factor / 糖鎖 / グリコシダーゼ
148.	Toshifumi Tomoyasu, Yukihiro Kaneko, Atsushi Tabata and Hideaki Nagamune : Identification and study of the binding region of ily transcriptional repressive element, 第87回日本細菌学会総会, Mar. 2014. (Keyword) Streptococci / 転写抑制因子結合部位 / gene expression / cytolysin
149.	Shu Murakami, Atsushi Tabata, 髙尾亞由子, Hisashi Ohkuni, Toshifumi Tomoyasu and Hideaki Nagamune : 高病原性Streptococcus mitisの検出・同定を目的とした新規検査システムの開発, 第87回日本細菌学会総会, Mar. 2014. (Keyword) Streptococci / identification / PCR / cytolysin
150.	Naoki Yamamoto, Toshifumi Tomoyasu, 今木英統, Atsushi Tabata and Hideaki Nagamune : Characterization of a novel secreted glycosidase (MsgA) of Streptococcus intermedius, 第87回日本細菌学会総会, Mar. 2014. (Keyword) Streptococci / グリコシダーゼ / 糖鎖 / decomposition
151.	Natsuki Anyohji, Atsushi Tabata, 大久保行将, Toshifumi Tomoyasu and Hideaki Nagamune : Comparison of the transition activity of Sortase A using GFP-substrate panel, 第87回日本細菌学会総会, Mar. 2014. (Keyword) enzyme / ペプチド転位酵素 / GFP
152.	金惠珍, Atsushi Tabata, Toshifumi Tomoyasu, 上野友美, 内山成人, Keizo Yuasa, Akihiko Tsuji and Hideaki Nagamune : エストロゲン作用物質が示す骨芽細胞分化促進作用の解析, 徳島生物学会, Dec. 2013. (Keyword) エストロゲン / 骨芽細胞分化
153.	大倉一人, 川口遊喜, Atsushi Tabata and Hideaki Nagamune : Streptococcus anginosus由来ストレプトリジンSホモログの分子内環形成による活性制御, 第17回バイオ治療法研究会学術集会, Dec. 2013. (Keyword) bacteria / ストレプトリジンS / Streptococcus anginosus
154.	Atsushi Tabata, 安養寺夏希, 大久保行将, Toshifumi Tomoyasu and Hideaki Nagamune : 細菌由来ペプチド転移酵素Sortase Aを用いたDDS用新規機能性リポソームの創成に関する検討, 第17回バイオ治療法研究会学術集会, Dec. 2013. (Keyword) bacteria / drug delivery system / liposomes / ペプチド転移酵素 / GFP
155.	Toshifumi Tomoyasu, Hidenori Imaki, Naoki Yamamoto, Atsushi Tabata and Hideaki Nagamune : Identification and characterization of MsgA, a novel secreted glycosidase of Streptococcus intermedius, 第36回日本分子生物学会年会, Dec. 2013. (Keyword) Streptococcus / pathogenic microorganism / sugar / enzyme
156.	Shu Murakami, Atsushi Tabata, 髙尾亞由子, Toshifumi Tomoyasu, Hisashi Ohkuni and Hideaki Nagamune : 高病原性Streptococcus mitis検出・同定システムの構築, 第66回日本細菌学会中国・四国支部総会, Oct. 2013. (Keyword) Streptococcus / pathogenic microorganism / identification / PCR
157.	Naoki Yamamoto, Toshifumi Tomoyasu, 今木英統, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermediusが分泌する新規グリコシダーゼの発見と性状解析, 第66回日本細菌学会中国・四国支部総会, Oct. 2013. (Keyword) Streptococcus / pathogenic microorganism / sugar / enzyme
158.	Natsuki Anyohji, Atsushi Tabata, Yukimasa Okubo, Toshifumi Tomoyasu and Hideaki Nagamune : 細菌由来ペプチド転移酵素Sortase Aの比較解析 -新規DDS開発への応用を目指して-, 日本生化学会, Sep. 2013. (Keyword) bacteria / drug delivery system / 蛍光タンパク質 / ペプチド転移酵素
159.	Nozomi Takeda, Atsushi Tabata, Asuka Nogami, 髙尾亞由子, Hisashi Ohkuni, 前田伸子, Toshifumi Tomoyasu and Hideaki Nagamune : N末に追加ドメインを有するコレステロール依存性細胞溶解毒素の作用特性, 日本生化学会, Sep. 2013. (Keyword) cytolysin / protein / cholesterol / sugar
160.	Atsushi Tabata, Kazuto Ohkura, Toshifumi Tomoyasu, Hisashi Ohkuni and Hideaki Nagamune : コレステロール依存性細胞溶解毒素の受容体認識および作用特性における多様性, 第60回毒素シンポジウム, Jul. 2013. (Keyword) Streptococcus / pathogenic microorganism / gene expression / virulence factor
161.	Toshifumi Tomoyasu, Hidenori Imaki, Naoki Yamamoto, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermedius の主要病原因子であるインターメディリシンの発現調節機構の解析, 第60回毒素シンポジウム, Jul. 2013. (Keyword) Streptococcus / pathogenic microorganism / gene expression / virulence factor
162.	Atsushi Tabata, 中野晃太, Kazuto Ohkura, 菊池賢, Toshifumi Tomoyasu and Hideaki Nagamune : β溶血性 Streptococcus anginosus が保有するストレプトリジンS ホモログの特徴と細胞障害性への寄与, 第22回 Lancefieldレンサ球菌研究会, Jun. 2013. (Keyword) Streptococcus / pathogenic microorganism / virulence factor
163.	Hidenori Imaki, Toshifumi Tomoyasu, 増田早智子, Naoki Yamamoto, Atsushi Tabata and Hideaki Nagamune : 糖タンパク質によるStreptococcus intermediusのily発現促進機構の解析, 第22回 Lancefieldレンサ球菌研究会, Jun. 2013. (Keyword) Streptococcus / pathogenic microorganism / virulence factor / gene expression
164.	Toshifumi Tomoyasu, Hiroyuki Kondo, Masanori Oisugi, Atsushi Tabata and Hideaki Nagamune : グラム陽性菌と陰性菌のDnaKシャペロンシステムの比較, 第54回日本生化学会中国・四国支部例会, Jun. 2013. (Keyword) DnaK / ClpB / Gram-Positive Bacteria / Gram-Negative Bacteria / molecular chaperone
165.	Hyejin Kim and Hideaki Nagamune : Effect of Estrogen-Stimuli on Osteoblast Differentiation, 2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research, May 2013.
166.	Asuka Nogami, Atsushi Tabata, Nozomi Takeda, Ayuko Takao, Hisashi Ohkuni, Nobuko Maeda, Toshifumi Tomoyasu and Hideaki Nagamune : β溶血性の高病原性Streptococcus mitisサブグループの特性, 第86回日本細菌学会総会, Mar. 2013. (Keyword) Streptococci / cytolysin / clinical laboratory testing
167.	Yukihiro Kaneko, Toshifumi Tomoyasu, Hidenoro Imaki, Atsushi Tabata and Hideaki Nagamune : ily転写抑制因子の結合領域の探索, 第86回日本細菌学会総会, Mar. 2013. (Keyword) Streptococci / cytolysin / gene expression
168.	Atsushi Tabata, Yuji Sato, Kota Nakano, Kazuto Ohkura, Ken Kikuti, Toshifumi Tomoyasu and Hideaki Nagamune : S. constellatus subsp. constellatusが保有するβ溶血因子の探索, 第86回日本細菌学会総会, Mar. 2013. (Keyword) Streptococci / cytolysin / enzyme
169.	Nozomi Takeda, Atsushi Tabata, Asuka Nogami, Ayuko Takao, Hisashi Ohkuni, Nobuko Maeda, Toshifumi Tomoyasu and Hideaki Nagamune : 新規5ドメイン型コレステロール依存性細胞溶解毒素の分子特性, 第86回日本細菌学会総会, Mar. 2013. (Keyword) Streptococci / cytolysin / enzyme
170.	Miku Ishikawa, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : ヒト特異的な膜孔形成毒素インターメディリシンにより好中球に誘導される応答反応, 第86回日本細菌学会総会, Mar. 2013. (Keyword) Streptococci / cytolysin / 細胞感染
171.	Hidenori Imaki, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Identification of an expressionpromoting factor for ily of Streptococcus intermedius, 第86回日本細菌学会総会, Mar. 2013. (Keyword) Streptococci / cytolysin / gene expression
172.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Does DnaK from Gram-positive Bacterium recognize ClpB of E.coli?, 第86回日本細菌学会総会, Mar. 2013. (Keyword) molecular chaperone / Streptococcus / enzyme
173.	Ayuko Takao, Hideaki Nagamune and Nobuko Maeda : Streptococcus intermediusのcomXホモログのコンピーテンス誘導とバイオフィルム形成における機能, 第86回日本細菌学会総会, Mar. 2013.
174.	Kenji Nagataki, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Investigation on the procedure for enhancement of cellulase activity and its application, 日本生化学会, Dec. 2012. (Keyword) molecular chaperone / セルラーゼ / バイオエタノール / application
175.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Small heat shock protein AgsA is an effective stabilizer of enzyme activities, 日本生化学会, Dec. 2012. (Keyword) molecular chaperone / sHSP / enzyme
176.	Hyejin Kim, Atsushi Tabata, Toshifumi Tomoyasu, Tomomi Ueno, Naruhito Uchiyama, Keizo Yuasa, Akihiko Tsuji and Hideaki Nagamune : A study on the mechanism of osteoblast differentiation induced by estrogen-stimuli, 日本生化学会, Dec. 2012. (Keyword) osteoblast / エストロゲン / イソフラボン
177.	大倉一人, 川口遊喜, Atsushi Tabata, 篠原康雄 and Hideaki Nagamune : 細菌由来細胞溶解毒素のヒト側受容体との相互作用, 第16回バイオ治療法研究会学術集会, Dec. 2012.
178.	Manami Aoyagi, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : インターメディリシンの細胞膜結合能と分子集合メカニズムの解析, 徳島生物学会, Dec. 2012. (Keyword) Streptococci / cytolysin / 細胞膜
179.	Yasunori Iwano, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : インターメディリシン刺激が単球系細胞THP-1の遺伝子発現に及ぼす影響, 徳島生物学会, Dec. 2012. (Keyword) Streptococci / cytolysin / gene expression
180.	Masanori Oisugi, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : 大腸菌のClpBは連鎖球菌のDnaKシャペロンシステムを認識するのか, 徳島生物学会, Dec. 2012. (Keyword) molecular chaperone / Hsp70 / Hsp104 / DnaK / ClpB
181.	Yukihiro Kaneko, Toshifumi Tomoyasu, Hidenori Imaki, Atsushi Tabata and Hideaki Nagamune : ily 発現抑制因子が認識するコンセンサス配列の探索, 日本細菌学会, Oct. 2012. (Keyword) Streptococci / gene expression / コンセンサス配列
182.	Atsushi Tabata, 中野晃太, 大倉一人, Toshifumi Tomoyasu, 菊池賢 and Hideaki Nagamune : β溶血性Streptococcus anginosusが保有するペプチド性溶血因子の分子特性, 第6回細菌学若手コロッセウム, Aug. 2012.
183.	金惠珍, Atsushi Tabata, Toshifumi Tomoyasu, 上野友美, 内山成人 and Hideaki Nagamune : Effect of Equol, an Enterobacterial Metabolite of Soybean Isoflavones on Osteoblast Differentiation., 第16回腸内細菌学会, Jun. 2012.
184.	今木英統, Toshifumi Tomoyasu, 増田早智子, Atsushi Tabata and Hideaki Nagamune : LacRはStreptococcus intermediusのily発現の主要な制御因子である, 第21回 Lancefieldレンサ球菌研究会, Jun. 2012.
185.	Atsushi Tabata, 野上明日香, 竹田望, 髙尾亞由子, Hisashi Ohkuni, Toshifumi Tomoyasu and Hideaki Nagamune : コレステロール依存性細胞溶解毒素遺伝子を保有するStreptococcus mitis株の毒素産生特性とヒト細胞障害性, 第21回 Lancefieldレンサ球菌研究会, Jun. 2012.
186.	金惠珍, Atsushi Tabata, Toshifumi Tomoyasu, 上野友美, 内山成人, Keizo Yuasa, Akihiko Tsuji and Hideaki Nagamune : エストロゲン作用物質が示す骨芽細胞の分化促進作用, 第53回日本生化学会中国・四国支部例会, May 2012.
187.	Toshifumi Tomoyasu, 今木英統, Atsushi Tabata and Hideaki Nagamune : グラム陽性菌と陰性菌のコシャペロンによるDnaK活性化機構の比較, 第85回日本細菌学会総会, Mar. 2012. (Keyword) Streptococci / molecular chaperone / protein
188.	今木英統, Toshifumi Tomoyasu, 増田早智子, Atsushi Tabata and Hideaki Nagamune : ILYを高産生するStreptococcus intermediusの臨床分離株の多くがLacRに変異を持つ, 第85回日本細菌学会総会, Mar. 2012. (Keyword) Streptococci / cytolysin / gene expression
189.	Atsushi Tabata, 中野晃太, Toshifumi Tomoyasu, 大倉一人, 菊池賢 and Hideaki Nagamune : TOMM family peptides decide the hemolytic character of Streptococcus anginosus, 第85回日本細菌学会総会, Mar. 2012. (Keyword) Streptococci / bacterial toxins
190.	高尾亞由子, Hideaki Nagamune and 前田伸子 : Streptococcus intermediusの生育に対するcomX遺伝子欠失の影響, 第85回日本細菌学会総会, Mar. 2012.
191.	福居孝之, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : コレステロール依存性細胞溶解毒素の膜孔形成反応における分子動態の解析, 第127回徳島生物学会, Dec. 2011. (Keyword) Streptococci / cytolysin
192.	Atsushi Tabata, 大久保行将, Toshifumi Tomoyasu and Hideaki Nagamune : 膜孔形成性キメラ毒素の新規DDSツールとしての応用性に関する検討, 第15回バイオ治療法研究会学術集会, Dec. 2011. (Keyword) cancer therapy
193.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermediusが保有するDnaKシャペロンシステムの機能解析, 第64回日本細菌学会中国・四国支部総会, Oct. 2011. (Keyword) Streptococci / molecular chaperone
194.	中野晃太, Atsushi Tabata, Toshifumi Tomoyasu, 大倉一人, 菊池賢, 平松啓一 and Hideaki Nagamune : β溶血性Streptococcus anginosusで見出された新規ペプチド性溶血因子, 第64回日本細菌学会中国・四国支部総会, Oct. 2011. (Keyword) Streptococci / bacterial toxins
195.	今木英統, Toshifumi Tomoyasu, 増田早智子, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermediusのily発現調節因子の探索, 第64回日本細菌学会中国・四国支部総会, Oct. 2011. (Keyword) Streptococci / cytolysin / gene expression
196.	大久保行将, Atsushi Tabata, 田村仁人, Toshifumi Tomoyasu and Hideaki Nagamune : 細胞標的化キメラ細菌毒素を用いた新規DDSツールの開発, 第84回日本生化学会大会, Sep. 2011. (Keyword) cancer therapy
197.	岡久奈緒美, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : キノコβ-グルカンによるマクロファージ様THP-1の活性化作用, 第84回日本生化学会大会, Sep. 2011. (Keyword) cancer immunotherapy
198.	Atsushi Tabata, 中野晃太, Toshifumi Tomoyasu and Hideaki Nagamune : アンギノーサス群連鎖球菌が保有する溶血因子に関する検討, 第5回細菌学若手コロッセウム, Aug. 2011. (Keyword) Streptococci / bacterial toxins
199.	Toshifumi Tomoyasu, 今木英統, 増田早智子, Atsushi Tabata and Hideaki Nagamune : コレステロール依存性細胞溶解毒素intermedilysinの発現調節因子がStreptococcus intermediusの病原性に果たす役割の解析, 第58回トキシンシンポジウム, Jul. 2011. (Keyword) Streptococci / cytolysin
200.	Atsushi Tabata, 中野晃太, Toshifumi Tomoyasu and Hideaki Nagamune : アンギノーサス群連鎖球菌が保有する溶血因子の探索, 第20回Lancefieldレンサ球菌研究会/第43回レンサ球菌感染症研究会, Jun. 2011.
201.	Hideaki Nagamune : 連鎖球菌が持つコレステロール依存性細胞溶解毒素, 第20回Lancefieldレンサ球菌研究会/第43回レンサ球菌感染症研究会, Jun. 2011.
202.	Toshifumi Tomoyasu, Hidenori Imaki, Atsushi Tabata and Hideaki Nagamune : カタボライト抑制因子CcpAによるStreptococcus intermediusのヒト特異的細胞溶解毒素intermedilysinの発現調節, 第33回日本分子生物学会年会第83回日本生化学会大会合同大会, Dec. 2010.
203.	Atsushi Tabata, 山本泰裕, Toshifumi Tomoyasu, Hisashi Ohkuni and Hideaki Nagamune : 2種のコレステロール依存性細胞溶解毒素を産生するStreptococcus mitis Nm-65株の細胞障害性に関する検討, 第33回日本分子生物学会年会第83回日本生化学会大会合同大会, Dec. 2010.
204.	田村仁人, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : 細菌由来タンパク質をベースとしたDDSツールの開発-標的細胞に対する選択的送達能に関する検討-, 第125回徳島生物学会, Dec. 2010.
205.	村田貴洋, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : コレステロール依存性細胞溶解毒素による宿主細胞の応答反応の検討, 第125回徳島生物学会, Dec. 2010.
206.	Atsushi Tabata, Kota Nakano, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus anginosusが保有する細胞傷害因子に関する検討, 第63回日本細菌学会中国・四国支部総会, Oct. 2010.
207.	増田早智子, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : 血液成分によるily遺伝子の発現調節, 第63回日本細菌学会中国・四国支部総会, Oct. 2010.
208.	山本泰裕, Atsushi Tabata, Toshifumi Tomoyasu, Katsuhiko Hirota, 大国寿士 and Hideaki Nagamune : 川崎病患児由来Streptococcus mitis Nm-65株が産生する2種類のコレステロール依存性細胞溶解毒素遺伝子の発現とその病原性への寄与, 第57回トキシンシンポジウム, Jul. 2010.
209.	Atsushi Tabata, 鈴木圭祐, Toshifumi Tomoyasu and Hideaki Nagamune : 細胞膜構造に依存したインターメディリシンの作用特性, 第51回日本生化学会中国・四国支部例会, May 2010.
210.	今木英統, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermedius ccpA破壊株とcre変異株の細胞毒性の解析, 第83回日本細菌学会総会, Mar. 2010. (Keyword) virulence factor
211.	Toshifumi Tomoyasu, 今木英統, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermedius dnaK破壊相補株の表現型の解析, 第83回日本細菌学会総会, Mar. 2010. (Keyword) virulence factor
212.	Shizuo Kayama, Keiji Murakami, Tsuneko Ono, 山本明毅, 谷口友伯, Katsuhiko Hirota, Hideaki Nagamune and Yoichiro Miyake : The role of QS Las-system and sigma factors in antibiotic tolerance in Pseudomonas aeruginosa, 第83回日本細菌学会総会, Mar. 2010.
213.	山本泰裕, Atsushi Tabata, Toshifumi Tomoyasu, Katsuhiko Hirota, Yoichiro Miyake, Hideaki Nagamune and 大國寿士 : Analysis of a novel S.mitis strain possessing two distinct cholesterol-dependent cytolysin genes, 第83回日本細菌学会総会, Mar. 2010.
214.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Dissection of the chaperone function of the small heat shock protein AgsA, 第32回日本分子生物学会年会, Dec. 2009.
215.	Atsushi Tabata, 金惠珍, Toshifumi Tomoyasu, Hiroki Kourai, 澤田千恵子, 下野生世, Motoyuki Sugai, 赤松優 and Hideaki Nagamune : 融合PCRイムノクロマトグラフィーによる迅速微生物検査系の構築, 日本防菌防黴学会 2009年度若手の会, Dec. 2009.
216.	Atsushi Tabata, 三好宗一郎, 天河崇, Toshifumi Tomoyasu and Hideaki Nagamune : 脂質ラフトを反応場とするStreptococcus intermediusのヒト細胞感染機構, 第82回日本生化学会大会, Oct. 2009.
217.	垣口貴沙, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : ヒト特異的細胞溶解毒素の膜貫入領域が膜孔形成反応において果たす機能の解析, 第82回日本生化学会大会, Oct. 2009.
218.	鈴木圭祐, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : 多様な受容体認識性を示すコレステロール依存性細胞溶解毒素の細胞膜構造に依存した作用特性, 第62回日本細菌学会中国・四国支部総会, Oct. 2009.
219.	伊藤貴洋, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : 蛍光性Streptococcus intermedius株の作製, 第62回日本細菌学会中国・四国支部総会, Oct. 2009.
220.	谷口友伯, Shizuo Kayama, 山本明毅, Tsuneko Ono, Katsuhiko Hirota, Yoichiro Miyake and Hideaki Nagamune : 緑膿菌のcyclic-di-GMPと表現型変化の関与について, 第62回日本細菌学会中国・四国支部総会, Oct. 2009.
221.	Atsushi Tabata, Keisuke Suzuki, Toshifumi Tomoyasu, Hisashi Ohkuni and Hideaki Nagamune : 標的細胞膜構造がコレステロール依存性細胞溶解毒素の受容体認識多様性に及ぼす影響, 若手研究者育成のためのワークショップ若手コロセウム(III), Oct. 2009.
222.	Toshifumi Tomoyasu, Atsushi Tabata, 菊池賢, 佐々木崇, 馬場理, 平松啓一 and Hideaki Nagamune : Streptococcus intermediusが産生するヒト特異的細胞溶解毒素インターメディリシンの発現調節機構の解析, 第56回トキシンシンポジウム, 106-109, Aug. 2009.
223.	Toshifumi Tomoyasu, 廣島理樹, 今木英統, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermediusのily発現調節機構の解析, 第18回 Lancefield レンサ球菌研究会, Jun. 2009.
224.	Atsushi Tabata, 篠原由樹, 鈴木圭祐, Toshifumi Tomoyasu and Hideaki Nagamune : インターメディリシンの膜孔形成に携わる分子構造と機能に関する解析, 第18回 Lancefieldレンサ球菌研究会, Jun. 2009.
225.	Atsushi Tabata, 篠原由樹, Toshifumi Tomoyasu and Hideaki Nagamune : インターメディリシンの分子間相互作用に寄与する部分構造の解析, 第50回日本生化学会中国・四国支部例会, May 2009.
226.	Toshifumi Tomoyasu, Atsushi Tabata, 菊池賢, 佐々木崇, 馬場理, 平松啓一 and Hideaki Nagamune : カタボライト抑制因子CcpAによるヒト特異的細胞溶解毒素インターメディリシンの発現調節機構の解析, 第82回日本細菌学会総会, Mar. 2009.
227.	垣口貴沙, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : ヒト特異的な細胞溶解毒素インターメディリシン(ILY)の膜孔形成を誘導する構造変化領域の解析, 第82回日本日本細菌学会総会, Mar. 2009.
228.	金惠珍, Toshifumi Tomoyasu, Atsushi Tabata, 菅井基行, 赤松優 and Hideaki Nagamune : 融合PCRイムノクロマトグラフィーによる迅速細菌検査, 第82回日本細菌学会総会, Mar. 2009.
229.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Streptooccus intermediusのストレス誘導型シャペロンDnaKの機能解析, 第82回日本日本細菌学会総会, Mar. 2009.
230.	Katsuhiko Hirota, Shizuo Kayama, Hiromichi Yumoto, Atsushi Tabata, Hideaki Nagamune, Tsuneko Ono and Yoichiro Miyake : Intermedilysinにより誘導される小胞体異常とgp210の細胞内分布の変動, 第82回日本日本細菌学会総会, Mar. 2009.
231.	谷口友伯, Shizuo Kayama, 山本明毅, Tsuneko Ono, Katsuhiko Hirota, Yoichiro Miyake and Hideaki Nagamune : 緑膿菌臨床分離株の付着表現型におけるcyclic-di-GMPの関与について, 第82回日本日本細菌学会総会, Mar. 2009.
232.	山本明毅, Tsuneko Ono, Shizuo Kayama, 谷口友伯, Katsuhiko Hirota, Hideaki Nagamune and Yoichiro Miyake : 緑膿菌における抗菌薬抵抗性とQuorum sensing機構に関する研究 ∼蛍光染色法による解析∼, 第82回日本細菌学会総会, Mar. 2009.
233.	Shizuo Kayama, Tsuneko Ono, 山本明毅, 谷口友伯, Katsuhiko Hirota, Hideaki Nagamune and Yoichiro Miyake : 緑膿菌Quorum sensing機構とrpoS遺伝子がofloxacin抵抗性に及ぼす影響について, 第82回日本日本細菌学会総会, Mar. 2009.
234.	伊藤真理, Tsuneko Ono, Chieko Matsuura, Shizuo Kayama, Yoichiro Miyake, 山本明毅, 谷口友伯 and Hideaki Nagamune : 緑膿菌臨床分離株のバイオフィルム形成におけるcyclic-di-GMPの関与について, 第32回徳島県医学検査学会, Dec. 2008.
235.	齋藤静香, Atsushi Tabata, 坂倉永里子, 鈴木圭祐, 垣口貴沙, 篠原由樹, 福島江, Toshifumi Tomoyasu, Hisashi Ohkuni and Hideaki Nagamune : コレステロール依存性細胞溶解毒素の受容体認識特性に関する検討, 第31回日本分子生物学会年会第81回日本生化学会大会合同大会, Dec. 2008.
236.	坂倉永里子, Atsushi Tabata, 齋藤静香, Toshifumi Tomoyasu and Hideaki Nagamune : 癌標的化毒素を用いた癌療法のための効果的DDSの開発, 第31回日本分子生物学会年会第81回日本生化学会大会合同大会, Dec. 2008.
237.	廣島理樹, Toshifumi Tomoyasu, 小南章, Atsushi Tabata, 菊池賢, 佐々木崇, 馬場理, 平松啓一 and Hideaki Nagamune : Streptococcus intermediusが保有するヒト特異的細胞溶解毒素インターメディリシンをコードするily遺伝子のプロモーター領域の解析, 第31回日本分子生物学会年会第81回日本生化学会大会合同大会, Dec. 2008.
238.	石川洋子, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : サルモネラ属細菌が保有する小型分子シャペロンAgsAのN末端とC末端領域の機能解析, 第31回日本分子生物学会年会第81回日本生化学会大会合同大会, Dec. 2008.
239.	垣口貴沙, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : ヒト特異的な細胞溶解毒素インターメディリシンの膜孔形成に関与する分子構造の解析, 第120回徳島生物学会, Nov. 2008.
240.	Katsuhiko Hirota, Shizuo Kayama, Atsushi Tabata, Hideaki Nagamune, Tsuneko Ono and Yoichiro Miyake : Intermedilysinにより誘導されるヒト培養胆管上皮細胞死とgp210の細胞内分布の変動, 第61回日本細菌学会中国・四国支部総会, Oct. 2008.
241.	Atsushi Tabata, 齋藤静香, 坂倉永里子, 鈴木圭祐, 篠原由樹, 福島江, Toshifumi Tomoyasu, Hisashi Ohkuni and Hideaki Nagamune : コレステロール及びヒト型CD59を受容体とするCholesterol-dependent cytolysin:Sm-hPAFのコレステロール認識特性に関する検討, 第61回日本細菌学会中国・四国支部総会, Oct. 2008.
242.	三好宗一郎, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Streptococcus intermediusのヒト細胞感染現象におけるインターメディリシンと宿主細胞膜構造の役割, 第61回日本細菌学会中国・四国支部総会, Oct. 2008.
243.	Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : 連鎖球菌属が産生するコレステロール依存性細胞溶解毒素の受容体認識における多様性, 第2回細菌学若手コロッセウム, Aug. 2008.
244.	廣島理樹, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : ヒト特異的細胞溶解毒素インターメディリシンの発現調節機構の解析, 第17回 Lancefieldレンサ球菌研究会, Jul. 2008.
245.	Atsushi Tabata, 齋藤静香, 坂倉永里子, 鈴木圭祐, 山本泰裕, 篠原由樹, 福島江, Toshifumi Tomoyasu, Hisashi Ohkuni and Hideaki Nagamune : ヒトCD59指向性コレステロール依存性細胞溶解毒素の受容体認識特性に関する検討, 第17回 Lancefieldレンサ球菌研究会, Jul. 2008.
246.	Katsuhiko Hirota, Heni Susilowati, Hirohiko Okamura, Kaya Yoshida, Atsushi Tabata, Shizuo Kayama, Hiromichi Yumoto, Tatsuji Haneji, Hideaki Nagamune, Tsuneko Ono and Yoichiro Miyake : Intermedilysinにより誘導されるカルシウム振動と胆管上皮細胞死, 第17回Lancefielfレンサ球菌研究会 2008, Jul. 2008.
247.	石川洋子, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : サルモネラ属細菌が保有する小型分子シャペロンAgsAの機能解析, 第49回日本生化学会中国・四国支部例会, May 2008.
248.	垣口貴沙, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : ヒト特異的な細胞溶解毒素インターメディリシン(ILY)の膜孔形成に関わる部分構造に関する解析, 第49回日本生化学会中国・四国支部例会, May 2008.
249.	Atsushi Tabata, Souichirou Miyoshi, Toshifumi Tomoyasu and Hideaki Nagamune : 宿主細胞膜構造がStreptococcus intermediusのヒト細胞感染に及ぼす影響, 第81回日本細菌学会総会, Mar. 2008. (Keyword) Streptococcus / Lipid raft / infection
250.	Hideaki Nagamune, Toshifumi Tomoyasu and Atsushi Tabata : ヒト特異的な細胞溶解毒素インターメディリシンの発現調節の遺伝学的解析, 第81回日本細菌学会総会, Mar. 2008.
251.	関矢加智子, 安部章夫 and Hideaki Nagamune : 溶血毒素ストレプトリジンOのドメイン3の膜貫入による溶血機構の超微形態学的解析, 第81回日本細菌学会総会, Mar. 2008.
252.	Hisashi Ohkuni, 留目優子, 高橋秀実 and Hideaki Nagamune : Streptococcus mitis由来ヒト血小板凝集因子(Sm-hPAF)に関する研究, 第81回日本細菌学会総会, Mar. 2008.
253.	Katsuhiko Hirota, Shizuo Kayama, Atsushi Tabata, Hideaki Nagamune, Tsuneko Ono and Yoichiro Miyake : Intermedilysinにより誘導される非アポトーシス型細胞死と細胞内Ca²⁺濃度の変動, 第81回日本細菌学会総会, Mar. 2008.
254.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : 連鎖球菌(S. intermedius)ClpP破壊株の作成とその表現型の解析, 第81回日本細菌学会総会, Mar. 2008. (Keyword) Streptococcus / protease / ClpP
255.	Atsushi Tabata, Souichirou Miyoshi, Ayuha Kadota, Kou Fukushima, Akiko Sukeno, Toshifumi Tomoyasu, Katsuhiko Hirota, Yoichiro Miyake, Hisashi Ohkuni and Hideaki Nagamune : 細菌感染反応場としてのヒト型CD59:ヒト細胞指向性コレステロール依存性細胞溶解毒素の受容体及びStreptococcus intermedius感染の宿主側トリガー因子としての役割, 第80回日本生化学会大会-第30回日本分子生物学会年会合同大会, Dec. 2007. (Keyword) Streptococcus / CD59 / Infection
256.	Hiroshima Riki, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermediusが生産するヒト特異的細胞溶解毒素インターメディシンの発現調節, 第80回日本生化学会大会-第30回日本分子生物学会年会合同大会, Dec. 2007. (Keyword) Streptococcus / gene expression
257.	Toshifumi Tomoyasu, Syunsuke Azuma, Hidenori Imaki, Atsushi Tabata and Hideaki Nagamune : 腸管出血性大腸菌のGrlAによるLEE1オペロンの新規発現調節機構, 第30回日本分子生物学会年会, Dec. 2007. (Keyword) EHEC / LEE pathgenisity island
258.	Katsuhiko Hirota, Susilowati Heni, 劉大力, Shizuo Kayama, Atsushi Tabata, Hideaki Nagamune, Tsuneko Ono and Yoichiro Miyake : Intermedilysinにより誘導されるヒト培養胆管上皮細胞死の分子機構の解明, 第60回日本細菌学会中国四国支部総会, Oct. 2007.
259.	Shinohara Yuki, Takumi Ishida, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Analysis of intermolecular interaction site of intermedilysin, 第60回日本細菌学会中国四国支部総会, Oct. 2007. (Keyword) Streptococcus / Cytolysin
260.	Atsushi Tabata, Miyoshi Souichirou, Kadota Ayuha, Akiko Sukeno, Katsuhiko Hirota, Toshifumi Tomoyasu, Yoichiro Miyake and Hideaki Nagamune : Streptococcus intermedius が分泌する細胞溶解毒素インターメディリシンが関与するヒト細胞特異的な感染現象, 第54回毒素シンポジウム, Sep. 2007. (Keyword) cytotoxicity / Streptococcus
261.	西木一生, 内山成人, 上野友美, 只野健太郎, Hideaki Nagamune and 吉田照豊 : 日本の海産魚類由来Lactococcus garvieaeとその他種由来株の遺伝疫学に関する研究, 第4回日本魚病学会, Sep. 2007.
262.	関矢加智子, 赤木巧, 龍田季代子, 端川勉, 安部章夫 and Hideaki Nagamune : ストレプトリジンOによる溶血機構の超微形態学的解析, 第54回毒素シンポジウム, Sep. 2007.
263.	Toshifumi Tomoyasu, Riki Hiroshima, Syunsuke Ishida, Akira Kominami, Atsushi Tabata and Hideaki Nagamune : Streptococcus intermediusの病原性発現調節機構の解析, 第16回Lancefieldレンサ球菌研究会, Jun. 2007. (Keyword) Streptococcus / Pathogenisity / Chaperon
264.	Atsushi Tabata, 三好宗一郎, Toshifumi Tomoyasu, Katsuhiko Hirota, Yoichiro Miyake and Hideaki Nagamune : 細胞溶解毒素インターメディリシンのヒト細胞特異的感染因子としての役割, 第80回日本細菌学会総会, Mar. 2007.
265.	今木英統, Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : GrlR，GrlAによる腸管出血性大腸菌(EHEC)のLEE領域発現制御機構の解析, 第80回日本細菌学会総会, Mar. 2007.
266.	Hideaki Maseda, 小中瑠実, Hideaki Nagamune, Tomoko Sumitomo, Takuya Maeda and Hiroki Kourai : Serratia marcescens の消毒剤および抗生物質に対する耐性機構の解明, 日本細菌学会, Mar. 2007.
267.	Katsuhiko Hirota, LIU Dali, Shizuo Kayama, Keiji Murakami, Hideaki Nagamune, Tsuneko Ono and Yoichiro Miyake : Intermedilysinによる培養ヒト胆管上皮細胞核膜抗原の異所性表出, 第80回日本細菌学会総会, Mar. 2007.
268.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : 腸管出血性大腸菌(EHEC)のLEE領域発現制御機構の解析, 第59回日本細菌学会中国・四国支部総, Oct. 2006.
269.	福島江, Atsushi Tabata, Toshifumi Tomoyasu, 大倉一人, 大国壽士 and Hideaki Nagamune : Sm-hPAFの受容体多様性:コレステロール依存性細胞溶解毒素の分子進化, 第59回日本細菌学会中国・四国支部総, Oct. 2006.
270.	Katsuhiko Hirota, LIU Dali, Shizuo Kayama, Keiji Murakami, Hideaki Nagamune and Yoichiro Miyake : Intermedilysinによるlamin B1の異所性表出, 第59回日本細菌学会中国・四国支部総会, Oct. 2006.
271.	Yuki Shinihara, 福島江, Toshifumi Tomoyasu, 大国壽士, 大倉一人 and Hideaki Nagamune : インターメディリシン及びそのホモローグの受容体認識部位の解析, 第79回日本細菌学会総会, Mar. 2006.
272.	Akira Kominami, Toshifumi Tomoyasu, Kou Fukushima and Hideaki Nagamune : ヒト特異的細胞溶解毒素インターメディリシンの発現調節機構の遺伝学的解析, 第79回日本細菌学会総会, Mar. 2006.
273.	Toshifumi Tomoyasu, 高屋明子, Hideaki Nagamune and 山本友子 : 腸管出血性大腸菌ClpXPプロテアーゼによるLEE pathogenic island 発現制御機構の解, 第79回日本細菌学会総会, Mar. 2006.
274.	渡辺裕子, Tomoko Sumitomo, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : セラチアの殺菌消毒剤耐性化機構, 日本防菌防黴学会, Nov. 2005.
275.	Oheni Susilowati, Katsuhiko Hirota, Dali Liu, Sizuo Kayama, Keiji Murakami, Ken Kikuchi, Kyoichi Totsuka, Hideaki Nagamune and Yoichiro Miyake : The Mechanism of Bile Epithelium Cell Death Under the Stimulation of Streptococcus Intermedius Cytolysin, 第58回日本細菌学会中国・四国支部総会, Oct. 2005.
276.	Tomoko Sumitomo, Akihiro Shirai, Hideaki Nagamune, Takuya Maeda and Hiroki Kourai : ビス型第四アンモニウム塩系薬剤の殺菌メカニズムの解明, 日本防菌防黴学会第32回年次大会, May 2005.
277.	Takuya Maeda, Hideaki Nagamune, Hiroki Kourai, 板東一宏 and 米本謙悟 : 銀系抗菌材のトマト養液栽培病原菌に対する殺菌効果, 日本防菌防黴学会第32回年次大会, May 2005.
278.	Takuya Maeda, Hideaki Nagamune, Hiroki Kourai, 森孝, 阪口浩士, 鬼塚浩明 and 辻正光 : 人工バイオフィルムの構築とその定量的評価方法に関する検討, 日本防菌防黴学会第32回年次大会, May 2005.
279.	吉成安純, Akihiro Shirai, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : 新規gemini型第四アンモニウム塩殺菌剤の合成とその抗菌活性, 日本防菌防黴学会第32回年次大会, May 2005.
280.	栗本麻友子, 藪原忠男, Akihiro Shirai, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Saccharomycesに対するビス型第四アンモニウム塩系薬剤の殺菌特性, 日本防菌防黴学会第32回年次大会, May 2005.
281.	浅田舞子, 藪原忠男, Akihiro Shirai, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : ビス型第四アンモニウム塩系薬剤の殺カビ特性, 日本防菌防黴学会第32回年次大会, May 2005.
282.	藪原忠男, Akihiro Shirai, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : 新規ビス型第四アンモニウム塩の抗菌活性とその溶血活性, 日本防菌防黴学会第32回年次大会, May 2005.
283.	藪原忠男, Akihiro Shirai, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : 新規ビス型第四アンモニウム塩の合成とその抗菌活性, 日本防菌防黴学会第32回年次大会, May 2005.
284.	犬伏晃子, Hideaki Nagamune, Kazuto Ohkura, 関矢加智子, Takuya Maeda and Hiroki Kourai : インターメディリシンとその受容体の結合に及ぼすコレステロールの影響, 日本細菌学会第78回総会, Apr. 2005.
285.	石田巧, Hideaki Nagamune, Kazuto Ohkura, Takuya Maeda and Hiroki Kourai : インターメディリシンに対する単クローン抗体の作成とその性質, 日本細菌学会第78回総会, Apr. 2005.
286.	浜川智江, Takuya Maeda, Hideaki Nagamune, Hiroki Kourai and 曽田勇作 : 塵埃に付着した浮遊細菌に対する銀担持抗菌フィルターの殺菌評価, 日本防菌防黴学会 2004年度若手の会, Oct. 2004.
287.	唐木真理, Akihiro Shirai, Takuya Maeda, Hideaki Nagamune, Hiroki Kourai, 山中一也, 西田守 and 福士英明 : ポリリジンの抗菌特性, 日本防菌防黴学会 2004年度若手の会, Oct. 2004.
288.	渡辺裕子, Akihiro Shirai, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : セラチアの殺菌消毒剤耐性化機構の解明, 日本防菌防黴学会 2004年度若手の会, Oct. 2004.
289.	Tomoko Sumitomo, Akihiro Shirai, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : ビス型第四アンモニウム塩系殺菌剤の殺菌メカニズムの解明, 日本防菌防黴学会 2004年度若手の会, Oct. 2004.
290.	吉成安純, 藪原忠男, Akihiro Shirai, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : 新規gemini型第四アンモニウム塩系殺菌剤の合成とその抗菌活性, 日本防菌防黴学会 2004年度若手の会, Oct. 2004.
291.	野々宮正晃, 藪原忠男, Akihiro Shirai, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Carbamate結合を有するgemini型第四アンモニウム塩系殺菌剤の合成とその抗菌活性, 日本防菌防黴学会 2004年度若手の会, Oct. 2004.
292.	Katsuhiko Hirota, 劉大力, 鹿山鎭男, Keiji Murakami, Ken Nemoto, Akiko Sukeno, Hideaki Nagamune, 菊池賢, 戸塚恭一 and Yoichiro Miyake : 抗Streptococcus intermedius HU型ヒストン様タンパク質抗体のHepG2細胞内ミトコンドリアとの反応性, 第57回日本細菌学会中国・四国支部総会, Oct. 2004.
293.	Takuya Maeda, 吉田あゆみ, Hideaki Nagamune and Hiroki Kourai : セラチアの殺菌消毒剤耐性化に関する検討, 日本防菌防黴学会第31回年次大会, Vol.IIDp-11, May 2004.
294.	山口文子, Tomoko Sumitomo, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : ビス型第四級アンモニウム塩系薬剤の殺菌メカニズム, 日本防菌防黴学会第31回年次大会, Vol.IIDp-10, May 2004.
295.	野々下理絵, 小林菜穂子, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : ペプチド系抗菌物質の殺菌機構, 日本防菌防黴学会第31回年次大会, Vol.IIDp-9, May 2004.
296.	Tomoko Sumitomo, Hideaki Nagamune, Takuya Maeda and Hiroki Kourai : ビス型第四級アンモニウム塩系薬剤の細胞破壊作用とOmpEの関連性, 日本防菌防黴学会第31回年次大会, Vol.IIDp-8, May 2004.
297.	Akiko Sukeno, Hideaki Nagamune, Katsuhiko Hirota, Kazuto Ohkura, Takuya Maeda, Yoichiro Miyake and Hiroki Kourai : インターメディリシンはS. intermediusがHepG2細胞に侵入する際に必要不可欠な因子である, 日本細菌学会第77回総会, Apr. 2004.
298.	榊原恵美子, Hideaki Nagamune, Keiji Murakami, Takuya Maeda, Yoichiro Miyake and Hiroki Kourai : 緑膿菌外膜タンパク質OprRの抗生物質感受性への関与, 日本細菌学会第77回総会, Apr. 2004.
299.	周飛, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : ピぺラジン環を含有るするビス型四級アンモニウム塩の合成と殺菌特性, 日本防菌防黴学会第30回年次大会, May 2003.
300.	小林菜穂子, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : ペプチド系抗菌物質の分子設計, 日本防菌防黴学会第30回年次大会, May 2003.
301.	Takuya Maeda, 怡土大志, 田中伸吾, Hideaki Nagamune and Hiroki Kourai : 第四アンモニウム塩系薬剤のアルキル鎖長と大腸菌の薬剤耐性化の関連性, 日本防菌防黴学会第30回年次大会, May 2003.
302.	Hideaki Nagamune, 榊原恵美子, 田端厚之, Takuya Maeda, Hiroki Kourai, Keiji Murakami and Yoichiro Miyake : 第四アンモニウム塩系消毒剤に対する緑膿菌の耐性化には外膜蛋白質OryRが関与している, 日本防菌防黴学会第30回年次大会, May 2003.
303.	Akiko Sukeno, Hideaki Nagamune, Kazuto Ohkura, Takuya Maeda and Hiroki Kourai : S. intermediusが分泌するインターメディリシンの宿主標的細胞モデルに及ぼす影響, 日本細菌学会第76回総会, Apr. 2003.
304.	Hideaki Nagamune, Takuya Maeda, Keiji Murakami, Yoichiro Miyake and Hiroki Kourai : 第四アンモニウム塩系消毒剤に対する緑膿菌の耐性化機構:外膜タンパク質QRP01の関与, 日本細菌学会第76回総会, Apr. 2003.
305.	Hiromichi Yumoto, Katsuhiko Hirota, Hideaki Nagamune, Kazuto Ohkura, Keiji Murakami, 山田真義, Hideaki Nakae and Yoichiro Miyake : Streptococcus intermedius hitone-like proteinをコードする遺伝子のクローニングと発現, 第75回日本細菌学会総会, Apr. 2002.
306.	Katsuhiko Hirota, Hiromichi Yumoto, 村田広美, Kazuto Ohkura, Hideaki Nagamune, Ken Nemoto, Tsuneko Ono and Yoichiro Miyake : レンサ球菌のヒストン様蛋白質の構造解析, 第53回日本細菌学会中国・四国支部総会, Oct. 2000.
307.	Tetsuya Akamatsu, 吉田茂, Hideaki Nagamune, Akihiko Tsuji and Yoshiko Matsuda : ラット脳発生過程でのプロセシング酵素PACE4の発現, 日本生化学会第70回大会, Sep. 1997.
308.	Tetsuya Akamatsu, 吉田茂, 大黒成夫, Hideaki Nagamune, 長濱正巳, Akihiko Tsuji and Yoshiko Matsuda : プロセシング酵素PACE4Eのラット脳発生過程での発現, 日本生化学会第69回大会・日本分子生物学会第19回年会合同年会, Aug. 1996.
309.	Tetsuya Akamatsu, 吉田茂, 水野勝弘, 長田香弥, 米本佳余子, 森健二, Hideaki Nagamune, Akihiko Tsuji, Eric Grouzmann and Yoshiko Matsuda : Kexin様プロテアーゼPC7の中枢神経系における細胞特異的発現, 日本生化学会第68回大会, Sep. 1995.
310.	Akihiko Tsuji, Hideaki Nagamune, Tetsuya Akamatsu, 日根智恵美, 村松和明, 森健二, 玉井靖啓, 米本佳余子 and Yoshiko Matsuda : 新Kexin様プロテアーゼの構造と組織分布, 日本先天代謝異常学会第36回大会, Nov. 1994.
311.	Tetsuya Akamatsu, 今村恵美, Hideaki Nagamune, Akihiko Tsuji and Yoshiko Matsuda : 新しいkexinファミリープロテアーゼPC7の細胞特異的発現-in situ hybridizationによる解析, 日本細胞生物学会第47回大会, Sep. 1994.
312.	玉井靖啓, 村松和明, Tetsuya Akamatsu, 日根智恵美, 森健二, Hideaki Nagamune, Akihiko Tsuji, Keisuke Izumi and Yoshiko Matsuda : 新ペプチドホルモンプロセッシングプロテアーゼPACE4Cの細胞特異的発現, 日本細胞生物学会第47回大会, Sep. 1994.
313.	Hideaki Nagamune, Akihiko Tsuji, 村松和明, Tetsuya Akamatsu, 東根一也, 日根智恵美, Keisuke Izumi, 坂口剛正, 吉田哲也, Sumihare Noji and Yoshiko Matsuda : 甲状腺傍濾胞細胞におけるサチラーゼファミリープロテアーゼの発現, 日本生化学会第66回大会, Oct. 1993.
314.	Tetsuya Akamatsu, Akihiko Tsuji, Hideaki Nagamune and Yoshiko Matsuda : ラット肝α-マクログロブリン-プロテアーゼ複合体の性質, 日本生化学会第65回大会, Oct. 1992.
315.	Akihiko Tsuji, Tetsuya Akamatsu, 尾田領一, Hideaki Nagamune and Yoshiko Matsuda : ラット肝α-マクログロブリン-プロテアーゼ複合体の性質, 日本生化学会中四国支部第55回例会, Apr. 1992.

Et cetera, Workshop:

1.	Atsushi Tabata, YOKOHATA Shuto, 大倉一人, Toshifumi Tomoyasu and Hideaki Nagamune : ペプチド溶血毒素ストレプトリジンSの血液成分による安定化と病原性への寄与, 第53回レンサ球菌研究会, Jul. 2023.
2.	Takashi Amoh, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Katsuhiko Hirota, Yoichiro Miyake and Hideki Fujii : 幼児口腔内から検出された多剤耐性レンサ球菌について, 第71回日本細菌学会中国，四国支部総会, Oct. 2018.
3.	Atsushi Tabata, 安養寺夏希, 大久保行将, 北條恵子, 水品善之, Toshifumi Tomoyasu and Hideaki Nagamune : Development of surface-processing systems as targeting technology of nano-particles, --- Optimization of the transpeptidase reaction of bacterial sortase A using GFP-substrate panel and investigation for its applications ---, 神戸学院大学ライフサイエンス産学連携研究センター, Mar. 2014.
4.	Atsushi Tabata, 大久保行将, 安養寺夏希, Toshifumi Tomoyasu and Hideaki Nagamune : Development of surface-processing systems as targeting technology of nano-particles, --- Development and investigation of SrtA substrate-producing system for surface processing ---, 神戸学院大学ライフサイエンス産学連携研究センター, Mar. 2013.
5.	Atsushi Tabata, 大久保行将, Toshifumi Tomoyasu and Hideaki Nagamune : 標的細胞に対する汎用性を高めたDDSツールの構築とその機能に関する評価, 第16回バイオ治療法研究会学術集会, Dec. 2012.
6.	Toshifumi Tomoyasu, Ayumi Okamoto, Hidenori Imaki, Atsushi Tabata and Hideaki Nagamune : ラクトースリプレッサーLacRによるインターメディリシン遺伝子の発現抑制, 第19回Lancefieldレンサ球菌研究会, Jun. 2010.
7.	Atsushi Tabata, 山本泰裕, Toshifumi Tomoyasu, Katsuhiko Hirota, Yoichiro Miyake, 大国寿士 and Hideaki Nagamune : 2種のコレステロール依存性細胞溶解毒素を産生する川崎病患児由来Streptococcus mitis Nm-65株の病原性に関する検討, 第19回Lancefieldレンサ球菌研究会, Jun. 2010.
8.	Akihiro Shirai, Shoko Ueta, Hideaki Nagamune and Hiroki Kourai : The Mode of the Antifungal Activity of Gemini-pyridinium Salt against, 神戸学院大学ライフサイエンス産業連携研究センター研究成果発表会, Sep. 2009.
9.	Hiroki Kourai and Hideaki Nagamune : 高機能性ジェミニ型殺菌剤の分子設計, 神戸学院大学ライフサイエンス産業連携研究センター研究成果発表会, Aug. 2007.
10.	Katsuhiko Hirota, 村田広美, Hiromichi Yumoto, 大倉一人, Hideaki Nagamune and Yoichiro Miyake : レンサ球菌のヒストン様蛋白質の構造解析, Bacterial Adherence & Biofilm, Vol.14, 6-8, Jan. 2000.

Report:

1.	Yoshihiko Ueno, Yosuke Seki, keiji Ido, Yasushi Takagi, Hiroshi Sakaue, Hitoshi Ikushima, Hideki Fujii, Yasuhiko Shirayama, Hideji TANAKA, Masatake Kawada, Hideaki Nagamune, Ray S. Furuya and Maiko Kamioka : 令和元年度徳島大学高等教育研究センターアドミッション部門報告書, 令和元年度徳島大学高等教育研究センターアドミッション部門報告書, Mar. 2020.

Patent:

1.	Hideaki Nagamune : コレステロール依存性細胞溶解毒素の変異体及びそのDDSへの利用, 2011-51295, .
2.	Hideaki Nagamune, Toshifumi Tomoyasu and Atsushi Tabata : Cholesterol-dependent cytolysin variant and use thereof in DDS, WO2012/121395 A1 (Sep. 2012), .
3.	Hideaki Nagamune : 二重標識融合PCRイムノクロマトグラフィー, 2008-005003 (Jan. 2008), 2009-165371 (Jul. 2009), 5435687 (Dec. 2013).
4.	Hiroki Kourai, Takuya Maeda, Hideaki Nagamune and 小林菜穂子 : 新規抗菌性ペプチドとその利用, 2003-359002 (Oct. 2003), 2005-120050 (May 2005), .
5.	Hiroki Kourai, Takuya Maeda, Hideaki Nagamune and 小林菜穂子 : 新規抗菌性ポリペプチドとその利用, 2002-344538 (Nov. 2002), 2004-175727 (Jun. 2004), 4154218 (Jul. 2008).
6.	Hideaki Nagamune : イムノクロマトグラフ分析の測定方法, 2001-394079 (Dec. 2001), .
7.	Hideaki Nagamune, Hiroki Kourai, 寺尾敏光 and 庄司英克 : ヒト細胞膜結合蛋白, 2001-176699 (Jun. 2001), 2002-360263 (Dec. 2002), .
8.	Hideaki Nagamune and Hiroki Kourai : ストレプトコッカス·インターメディウスの検出方法及びそれに用いるPCRプライマー対, 2000-151268 (May 2000), 2001-327300 (Nov. 2001), .
9.	Hiroki Kourai, Takuya Maeda, Hideaki Nagamune, 吉田宗弘, 槌谷浩司 and 難波哲人 : 抗菌活性を有するビス型第四級アンモニウム塩化合物及びその製造方法, H9-104021 (Apr. 1997), H10-287566 (Oct. 1998), .
10.	Hideaki Nagamune and Fusao Ota : ストレプトコッカス·ミュータンスに特異的に反応するモノクローナル抗体, 63-331740 (Dec. 1988), 2-177898 (Jul. 1990), 2749338 (Feb. 1998).

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

The environmental determinant for Anginosus group streptococci to be the human pathogen. (Project/Area Number: 20K09919 )
Molecular evolution of hemolysins involved in oral streptococcal pathogenicity (Project/Area Number: 18K09552 )
Importance for the existence of mobile-genetic elements in cytotoxic Anginosus group streptococci (Project/Area Number: 17K11615 )
Pathogenicity acquisition mechanism of oral streptococci via gene transfer and expression of cytolysin genes (Project/Area Number: 15K11012 )
Functional analysis of streptolysin S involved factors (Project/Area Number: 15K08113 )
Role of a novel secreted glycosidase for pathogenicity of a human-specific pathogenic streptococci. (Project/Area Number: 26460528 )
Highly pathogenic Streptococcus mitis exists: A study on its pathogenicity and epidemiology (Project/Area Number: 24592769 )
Molecular analysis of streptococcus derived protein factors for application to drug discovery (Project/Area Number: 24590221 )
Investigation of the regulatory mechanism for the production of a human specific cytolysin, a crucial factor for the infectivity of Streptococcus intermedius (Project/Area Number: 23590510 )
Development of a cancer treatment technique using intracellular collapsing liposomes directed to cancer with double safety systems (Project/Area Number: 21590115 )
Regulation of pathogenicity by the heat shock chaperone and the protease in streptococci (Project/Area Number: 19590449 )
Molecular mechanism of human-specific infection by an oral streptococcus, Streptococcusintermedius (Project/Area Number: 18592004 )
Expression control mechanisms of LEE pathogenic factors by AAA proteases in Enterohaemorrhagic and Enteropathogenic Eschericha coli (Project/Area Number: 17590387 )
A modification technique of cell membrane function using the membrane binding site of a cytolysin, intermedilysin (Project/Area Number: 14580822 )
Analysis of Intermedilysin in Human Specific Cytolytic Mechanism : Design and Application for Cell Targeting Module. (Project/Area Number: 12672151 )
ヒト細胞に特異的な細胞毒素intermedilysinによる細胞死のメカニズム-類人猿から人類への進化は細胞にどのような変化をもたらしたか?- (Project/Area Number: 08878100 )
生理活性ペプチドプロセシングプロテアーゼ・PACE4の機能 (Project/Area Number: 07680689 )
新Kex2様プロテアーゼの同定と構造及びその生理機能の研究 (Project/Area Number: 06680617 )
前駆体タンパク質の小胞体ゴルジ系におけるプロセシングの細胞特異性の研究 (Project/Area Number: 06248102 )
炎症時のαマクログログリン-肥満細胞トリプターゼ複合体の機能 (Project/Area Number: 05807011 )
膜融合タンパク質の活性化とウイルス感染のトポロジー (Project/Area Number: 05670143 )
ウイルス膜融合タンパク質の宿主細胞内輸送とトランスゴルジ領域での活性化機構 (Project/Area Number: 05252102 )
宿主細胞によるウイルスタンパク質活性化機構 (Project/Area Number: 04670163 )
ゴルジ領域に於けるウイルス膜融合タンパク質のブロシングとその生理的意義 (Project/Area Number: 04259105 )
Cloning the genes for Streptococcus mutans-specific antigens (Project/Area Number: 01571020 )
Study on the surface antigens of streptococcal cells. (Project/Area Number: 63570873 )
Search by Researcher Number (40189163)

Professor Emeritus : Nagamune, Hideaki

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)