Ayuko Sakane and Takuya Sasaki : Roles of Rab family small G proteins in formation of the apical junctional complex in epithelial cells., Cell Polarity: Biological Role and Basic Mechanisms, Germany, Feb. 2015.
Taka-aki Yano, Taira Kajisa, Masayuki Ono, Yoshiya Miyasaka, Yuichi Hasegawa, Atsushi Saito, Kunihiro Otsuka, Ayuko Sakane, Takuya Sasaki, Koji Yasutomo, Rina Hamajima, Yuta Kanai, Takeshi Kobayashi, Yoshiharu Matsuura, Makoto Itonaga and Takeshi Yasui : Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance., Scientific Reports, Vol.12, No.1, 1060, 2022.
(Summary)
The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing the limit of detection (LOD) is revealed for exceptionally large gold nanoparticles (AuNPs) with diameters of hundreds of nm. SPR enhanced by these large nanoparticles lowered the LOD of SARS-CoV-2 N protein to 85 fM, resulting in the highest SPR detection sensitivity ever obtained for SARS-CoV-2 N protein.
Elongated tubular endosomes play essential roles in diverse cellular functions. Multiple molecules have been implicated in tubulation of recycling endosomes, but the mechanism of endosomal tubule biogenesis has remained unclear. In this study, we found that JRAB/MICAL-L2 induces endosomal tubulation via activated Rab8A. In association with Rab8A, JRAB/MICAL-L2 adopts its closed form, which functions in the tubulation of recycling endosomes. Moreover, JRAB/MICAL-L2 induces liquid-liquid phase separation, initiating the formation of tubular recycling endosomes upon overexpression. Between its N-terminal and C-terminal globular domains, JRAB/MICAL-L2 contains an intrinsically disordered region, which contributes to the formation of JRAB/MICAL-L2 condensates. Based on our findings, we propose that JRAB/MICAL-L2 plays two sequential roles in the biogenesis of tubular recycling endosomes: first, JRAB/MICAL-L2 organizes phase separation, and then the closed form of JRAB/MICAL-L2 formed by interaction with Rab8A promotes endosomal tubulation.
The Mon La, Hiromi Tachibana, Shun-Ai Li, Tadashi Abe, Sayaka Seiriki, Hikaru Nagaoka, Eizo Takashima, Tetsuya Takeda, Daisuke Ogawa, Shin-Ichi Makino, Katsuhiko Asanuma, Masami Watanabe, Xuefei Tian, Shuta Ishibe, Ayuko Sakane, Takuya Sasaki, Jun Wada, Kohji Takei and Hiroshi Yamada : Dynamin 1 is important for microtubule organization and stabilization in glomerular podocytes., The FASEB journal, Vol.34, No.12, 16449-16463, 2020.
(Summary)
Dynamin 1 is a neuronal endocytic protein that participates in vesicle formation by scission of invaginated membranes. Dynamin 1 is also expressed in the kidney; however, its physiological significance to this organ remains unknown. Here, we show that dynamin 1 is crucial for microtubule organization and stabilization in glomerular podocytes. By immunofluorescence and immunoelectron microscopy, dynamin 1 was concentrated at microtubules at primary processes in rat podocytes. By immunofluorescence of differentiated mouse podocytes (MPCs), dynamin 1 was often colocalized with microtubule bundles, which radially arranged toward periphery of expanded podocyte. In dynamin 1-depleted MPCs by RNAi, α-tubulin showed a dispersed linear filament-like localization, and microtubule bundles were rarely observed. Furthermore, dynamin 1 depletion resulted in the formation of discontinuous, short acetylated α-tubulin fragments, and the decrease of microtubule-rich protrusions. Dynamins 1 and 2 double-knockout podocytes showed dispersed acetylated α-tubulin and rare protrusions. In vitro, dynamin 1 polymerized around microtubules and cross-linked them into bundles, and increased their resistance to the disassembly-inducing reagents Ca and podophyllotoxin. In addition, overexpression and depletion of dynamin 1 in MPCs increased and decreased the nocodazole resistance of microtubules, respectively. These results suggest that dynamin 1 supports the microtubule bundle formation and participates in the stabilization of microtubules.
Shotaro Sakakibara, Kiyohito Mizutani, Ayumu Sugiura, Ayuko Sakane, Takuya Sasaki, Shigenobu Yonemura and Yoshimi Takai : Afadin regulates actomyosin organization through αE-catenin at adherens junctions., The Journal of Cell Biology, Vol.219, No.5, 2020.
(Summary)
Actomyosin-undercoated adherens junctions are critical for epithelial cell integrity and remodeling. Actomyosin associates with adherens junctions through αE-catenin complexed with β-catenin and E-cadherin in vivo; however, in vitro biochemical studies in solution showed that αE-catenin complexed with β-catenin binds to F-actin less efficiently than αE-catenin that is not complexed with β-catenin. Although a "catch-bond model" partly explains this inconsistency, the mechanism for this inconsistency between the in vivo and in vitro results remains elusive. We herein demonstrate that afadin binds to αE-catenin complexed with β-catenin and enhances its F-actin-binding activity in a novel mechanism, eventually inducing the proper actomyosin organization through αE-catenin complexed with β-catenin and E-cadherin at adherens junctions.
Kazuhisa Miyake, Ayuko Sakane, Ikuko Sagawa, Yoko Tomida, Jiro Kasahara and Takuya Sasaki : Actin Cytoskeletal Reorganization Function of JRAB/MICAL-L2 Is Fine-tuned by Intramolecular Interaction between First LIM Zinc Finger and C-terminal Coiled-coil Domains, Scientific Reports, Vol.9, No.1, 12794, 2019.
(Summary)
JRAB/MICAL-L2 is an effector protein of Rab13, a member of the Rab family of small GTPase. JRAB/MICAL-L2 consists of a calponin homology domain, a LIM domain, and a coiled-coil domain. JRAB/MICAL-L2 engages in intramolecular interaction between the N-terminal LIM domain and the C-terminal coiled-coil domain, and changes its conformation from closed to open under the effect of Rab13. Open-form JRAB/MICAL-L2 induces the formation of peripheral ruffles via an interaction between its calponin homology domain and filamin. Here, we report that the LIM domain, independent of the C-terminus, is also necessary for the function of open-form JRAB/MICAL-L2. In mechanistic terms, two zinc finger domains within the LIM domain bind the first and second molecules of actin at the minus end, potentially inhibiting the depolymerization of actin filaments (F-actin). The first zinc finger domain also contributes to the intramolecular interaction of JRAB/MICAL-L2. Moreover, the residues of the first zinc finger domain that are responsible for the intramolecular interaction are also involved in the association with F-actin. Together, our findings show that the function of open-form JRAB/MICAL-L2 mediated by the LIM domain is fine-tuned by the intramolecular interaction between the first zinc finger domain and the C-terminal domain.
Ryuta Nomiyama, Masahiro Emoto, Naofumi Fukuda, Kumiko Matsui, Manabu Kondo, Ayuko Sakane, Takuya Sasaki and Yukio Tanizawa : Protein kinase C iota facilitates insulin-induced glucose transport by phosphorylation of soluble nSF attachment protein receptor regulator (SNARE) double C2 domain protein b., Journal of Diabetes Investigation, Vol.10, No.3, 591-601, 2019.
(Summary)
Double C2 domain protein b (DOC2b), one of the synaptotagmins, has been shown to translocate to the plasma membrane, and to initiate membrane-fusion processes of vesicles containing glucose transporter 4 proteins on insulin stimulation. However, the mechanism by which DOC2b is regulated remains unclear. Herein, we identified the upstream regulatory factors of DOC2b in insulin signal transduction. We also examined the role of DOC2b on systemic homeostasis using DOC2b knockout (KO) mice. We first identified DOC2b binding proteins by immunoprecipitation and mutagenesis experiments. Then, DOC2b KO mice were generated by disrupting the first exon of the DOC2b gene. In addition to the histological examination, glucose metabolism was assessed by measuring parameters on glucose/insulin tolerance tests. Insulin-stimulated glucose uptake was also measured using isolated soleus muscle and epididymal adipose tissue. We identified an isoform of atypical protein kinase C (protein kinase C iota) that can bind to DOC2b and phosphorylates one of the serine residues of DOC2b (S34). This phosphorylation is essential for DOC2b translocation. DOC2b KO mice showed insulin resistance and impaired oral glucose tolerance on insulin and glucose tolerance tests, respectively. Insulin-stimulated glucose uptake was impaired in isolated soleus muscle and epididymal adipose tissues from DOC2b KO mice. We propose a novel insulin signaling mechanism by which protein kinase C iota phosphorylates DOC2b, leading to glucose transporter 4 vesicle translocation, fusion and facilitation of glucose uptake in response to insulin. The present results also showed DOC2b to play important roles in systemic glucose homeostasis.
Ayuko Sakane, Shin Yoshizawa, Hideo Yokota and Takuya Sasaki : Dancing Styles of Collective Cell Migration: Image-Based Computational Analysis of JRAB/MICAL-L2., Frontiers in Cell and Developmental Biology, Vol.6, 4, 2018.
(Summary)
Collective cell migration is observed during morphogenesis, angiogenesis, and wound healing, and this type of cell migration also contributes to efficient metastasis in some kinds of cancers. Because collectively migrating cells are much better organized than a random assemblage of individual cells, there seems to be a kind of order in migrating clusters. Extensive research has identified a large number of molecules involved in collective cell migration, and these factors have been analyzed using dramatic advances in imaging technology. To date, however, it remains unclear how myriad cells are integrated as a single unit. Recently, we observed unbalanced collective cell migrations that can be likened to either precision dancing or , Japanese traditional dancing similar to the style at Rio Carnival, caused by the impairment of the conformational change of JRAB/MICAL-L2. This review begins with a brief history of image-based computational analyses on cell migration, explains why quantitative analysis of the stylization of collective cell behavior is difficult, and finally introduces our recent work on JRAB/MICAL-L2 as a successful example of the multidisciplinary approach combining cell biology, live imaging, and computational biology. In combination, these methods have enabled quantitative evaluations of the "dancing style" of collective cell migration.
Ayuko Sakane, Shin Yoshizawa, Masaomi Nishimura, Yuko Tsuchiya, Natsuki Matsushita, Kazuhisa Miyake, Kazuki Horikawa, Issei Imoto, Chiharu Mizuguchi, Hiroyuki Saito, Takato Ueno, Sachi Matsushita, Hisashi Haga, Shinji Deguchi, Kenji Mizuguchi, Hideo Yokota and Takuya Sasaki : Conformational plasticity of JRAB/MICAL-L2 provides "law and order" in collective cell migration., Molecular Biology of the Cell, Vol.27, No.20, 3095-3108, 2016.
(Summary)
In fundamental biological processes, cells often move in groups, a process termed collective cell migration. Collectively migrating cells are much better organized than a random assemblage of individual cells. Many molecules have been identified as factors involved in collective cell migration, and no one molecule is adequate to explain the whole picture. Here we show that JRAB/MICAL-L2, an effector protein of Rab13 GTPase, provides the "law and order" allowing myriad cells to behave as a single unit just by changing its conformation. First, we generated a structural model of JRAB/MICAL-L2 by a combination of bioinformatic and biochemical analyses and showed how JRAB/MICAL-L2 interacts with Rab13 and how its conformational change occurs. We combined cell biology, live imaging, computational biology, and biomechanics to show that impairment of conformational plasticity in JRAB/MICAL-L2 causes excessive rigidity and loss of directionality, leading to imbalance in cell group behavior. This multidisciplinary approach supports the concept that the conformational plasticity of a single molecule provides "law and order" in collective cell migration.
Ayuko Sakane, Ahmed Mahmoud Alamir Abdallah, Kiyoshi Nakano, Kazufumi Honda, Toshio Kitamura, Issei Imoto, Natsuki Matsushita and Takuya Sasaki : Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins., Genes to Cells, Vol.18, No.9, 810-822, 2013.
(Summary)
We previously showed that Rab13 and its effector protein, junctional Rab13-binding protein (JRAB)/molecules interacting with CasL-like 2 (MICAL-L2), regulate junctional development by modulating cell adhesion molecule transport and actin cytoskeletal reorganization in epithelial cells. Here, we investigated how JRAB regulates reorganization of the actin cytoskeleton in NIH3T3 fibroblasts, in an attempt to obtain novel insights into the mechanism of JRAB action. To this end, we expressed mutant proteins that adopt a constitutively open or closed state and then examined effect on cellular morphology of the resulting actin cytoskeletal reorganization. Expression of the JRABΔCT mutant (constitutively 'closed' state) induced stress fibers, whereas expression of the JRABΔCC mutant (constitutively 'open' state) caused cell spreading with membrane ruffles. Next, we identified the proteins involved in JRAB-induced rearrangement of actin cytoskeleton leading to morphological changes. In NIH3T3 cells expressing HA-JRABΔCC, filamin, an actin cross-linking protein, coimmunoprecipitated with HA-JRABΔCC. Expression of ASB2 induced degradation of all three filamin isoforms and inhibited the JRABΔCC-induced cell spreading. Consistent with our previous results, actinin-1/-4 were also immunoprecipitated with HA-JRABΔCC. However, actinin-1/-4 have no effect on the cell spreading regulated by JRABΔCC. These data suggest that JRAB contributes to the rearrangement of the actin cytoskeleton during cell spreading via filamins.
Ayuko Sakane, Ahmed Alamir Mahmoud Abdallah, Kiyoshi Nakano, Kazufumi Honda, Wataru Ikeda, Yumiko Nishikawa, Mitsuru Matsumoto, Natsuki Matsushita, Toshio Kitamura and Takuya Sasaki : Rab13 small G protein and junctional Rab13-binding protein (JRAB) orchestrate actin cytoskeletal organization during epithelial junctional development., The Journal of Biological Chemistry, Vol.287, No.51, 42455-42468, 2012.
(Summary)
During epithelial junctional development, both vesicle transport and reorganization of the actin cytoskeleton must be spatiotemporally regulated. Coordination of these cellular functions is especially important, but the precise mechanism remains elusive. Previously, we identified junctional Rab13-binding protein (JRAB)/molecules interacting with CasL-like 2 (MICAL-L2) as an effector of the Rab13 small G protein, and we found that the Rab13-JRAB system may be involved in the formation of cell-cell adhesions via transport of adhesion molecules. Here, we showed that JRAB interacts with two actin-binding proteins, actinin-1 and -4, and filamentous actin via different domains and regulates actin cross-linking and stabilization through these interactions. During epithelial junctional development, JRAB is prominently enriched in the actin bundle at the free border; subsequently, JRAB undergoes a Rab13-dependent conformational change that is required for maturation of cell-cell adhesion sites. These results suggest that Rab13 and JRAB regulate reorganization of the actin cytoskeleton throughout epithelial junctional development from establishment to maturation of cell-cell adhesion.
(Keyword)
Actin Cytoskeleton / Actinin / Actins / Animals / Cell Line / Cell Membrane / Cytoskeletal Proteins / Epithelial Cells / Epithelium / Humans / Mice / Models, Biological / Protein Binding / Protein Multimerization / Protein Structure, Tertiary / Protein Transport / Tight Junctions / rab GTP-Binding Proteins
Kanchanamala Withanage, Kentaro Nakagawa, Mitsunobu Ikeda, Hidetake Kurihara, Takumi Kudo, Zeyu Yang, Ayuko Sakane, Takuya Sasaki and Yutaka Hata : Expression of RASSF6 in kidney and the implication of RASSF6 and the Hippo pathway in the sorbitol-induced apoptosis in renal proximal tubular epithelial cells., The Journal of Biochemistry, Vol.152, No.1, 111-119, 2012.
(Summary)
RASSF6, a member of RASSF tumour suppressor proteins, binds to mammalian Ste20-like kinases (MST1/2), core kinases of the proapoptotic Hippo pathway and cooperates with the Hippo pathway to induce apoptosis. We originally identified RASSF6 as a putative interactor of membrane-associated guanylate kinase inverted (MAGI)-1 by the yeast two-hybrid screening. We used human kidney cDNA library for the screening. MAGI-1 is abundantly expressed in kidney and is a core component of the slit diaphragm. These findings suggest that RASSF6 is expressed in kidney. However, the function of RASSF6 in kidney is not yet studied. We performed this study to confirm the interaction of RASSF6 with MAGI-1, to analyse the expression of RASSF6 in kidney and to gain insight into the function of RASSF6 in kidney. RASSF6 binds to PDZ domains of MAGI-1 through its C-terminal PDZ-binding motif and is coimmunoprecipitated with MAGI-1 from rat liver. RASSF6 is localized in normal kidney glomerulus but disappears when the slit diaphragm is disrupted in nephrotic kidney. RASSF6 is also localized on apical membranes in renal proximal tubular epithelial cells. We demonstrated that RASSF6 as well as the Hippo pathway are involved in the sorbitol-induced apoptosis in immortalized human proximal renal tubular epithelial HK-2 cells.
Keisuke Tabata, Kohichi Matsunaga, Ayuko Sakane, Takuya Sasaki, Takeshi Noda and Tamotsu Yoshimori : Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain., Molecular Biology of the Cell, Vol.21, No.23, 4162-4173, 2010.
(Summary)
The endocytic and autophagic pathways are involved in the membrane trafficking of exogenous and endogenous materials to lysosomes. However, the mechanisms that regulate these pathways are largely unknown. We previously reported that Rubicon, a Beclin 1-binding protein, negatively regulates both the autophagic and endocytic pathways by unidentified mechanisms. In this study, we performed database searches to identify potential Rubicon homologues that share the common C-terminal domain, termed the RH domain. One of them, PLEKHM1, the causative gene of osteopetrosis, also suppresses endocytic transport but not autophagosome maturation. Rubicon and PLEKHM1 specifically and directly interact with Rab7 via their RH domain, and this interaction is critical for their function. Furthermore, we show that Rubicon but not PLEKHM1 uniquely regulates membrane trafficking via simultaneously binding both Rab7 and PI3-kinase.
(Keyword)
Adaptor Proteins, Signal Transducing / Apoptosis Regulatory Proteins / Autophagy / Endocytosis / HeLa Cells / Humans / Immunoblotting / Intracellular Signaling Peptides and Proteins / Lysosomes / Membrane Glycoproteins / Membrane Proteins / Membrane Transport Modulators / Microscopy, Fluorescence / Phosphatidylinositol 3-Kinases / Protein Interaction Domains and Motifs / Protein Transport / rab GTP-Binding Proteins
Ayuko Sakane, Kazufumi Honda and Takuya Sasaki : Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2., Molecular and Cellular Biology, Vol.30, No.4, 1077-1087, 2010.
(Summary)
Neurite outgrowth is the first step in the processes of neuronal differentiation and regeneration and leads to synaptic polarization and plasticity. Rab13 small G protein shows an increased mRNA expression level during neuronal regeneration; it is therefore thought to be involved in this process. We previously identified JRAB (junctional Rab13-binding protein)/MICAL-L2 (molecules interacting with CasL-like 2) as a novel Rab13 effector protein. Here, we show that Rab13 regulates neurite outgrowth in the rat pheochromocytoma cell line PC12 through an interaction with JRAB/MICAL-L2. The expression of JRAB/MICAL-L2 alone inhibits neurite outgrowth, whereas coexpression of the dominant active form of Rab13 rescues this effect. We also demonstrate an intramolecular interaction between the N-terminal calponin-homology (CH) and LIM domains of JRAB/MICAL-L2 and the C-terminal coiled-coil domain. Finally, we show that the binding of Rab13 to JRAB/MICAL-L2 stimulates the interaction of JRAB/MICAL-L2 with actinin-4, an actin-binding protein, which localizes to the cell body and the tips of the neurites in PC12 cells. These results suggest that Rab13 and JRAB/MICAL-L2 may act to transfer actinin-4 from the cell body to the tips of neurites, where actinin-4 is involved in the reorganization of the actin cytoskeleton which results in neurite outgrowth.
Anita Szodorai, Yung-Hui Kuan, Silke Hunzelmann, Ulrike Engel, Ayuko Sakane, Takuya Sasaki, Yoshimi Takai, Joachim Kirsch, Ulrike Muller, Konrad Beyreuther, Scott Brady, Gerardo Morfini and Stefan Kins : APP Anterograde Transport Requires Rab3A GTPase Activity for Assembly of the Transport Vesicle, The Journal of Neuroscience, Vol.29, No.46, 14534-14544, 2009.
(Summary)
The amyloid precursor protein (APP) is anterogradely transported by conventional kinesin in a distinct transport vesicle, but both the biochemical composition of such a vesicle and the specific kinesin-1 motor responsible for transport are poorly defined. APP may be sequentially cleaved by beta- and gamma-secretases leading to accumulation of beta-amyloid (Abeta) peptides in brains of Alzheimer's disease patients, whereas cleavage of APP by alpha-secretases prevents Abeta generation. Here, we demonstrate by time-lapse analysis and immunoisolations that APP is a cargo of a vesicle containing the kinesin heavy chain isoform kinesin-1C, the small GTPase Rab3A, and a specific subset of presynaptic protein components. Moreover, we report that assembly of kinesin-1C and APP in this vesicle type requires Rab3A GTPase activity. Finally, we show cleavage of APP in transport vesicles by alpha-secretase activity, likely mediated by ADAM10. Together, these data indicate that maturation of APP transport vesicles, including recruitment of conventional kinesin, requires Rab3 GTPase activity.
(Keyword)
Amyloid beta-Protein Precursor / Animals / Base Sequence / Cell Line, Tumor / Enzyme Activation / GTP Phosphohydrolases / Humans / Kinesin / Mice / Mice, Inbred C57BL / Mice, Knockout / Molecular Sequence Data / Protein Transport / Transport Vesicles / rab3A GTP-Binding Protein
Hironori Higashio, Noriyuki Nishimura, Hiroyoshi Ishizaki, Jun Miyoshi, Satoshi Orita, Ayuko Sakane and Takuya Sasaki : Doc2α and Munc13-4 Regulate Ca2+-Dependent Secretory Lysosome Exocytosis in Mast Cells., The Journal of Immunology, Vol.180, No.7, 4774-4784, 2008.
(Summary)
The Doc2 family comprises the brain-specific Doc2alpha and the ubiquitous Doc2beta and Doc2gamma. With the exception of Doc2gamma, these proteins exhibit Ca(2+)-dependent phospholipid-binding activity in their Ca(2+)-binding C2A domain and are thought to be important for Ca(2+)-dependent regulated exocytosis. In excitatory neurons, Doc2alpha interacts with Munc13-1, a member of the Munc13 family, through its N-terminal Munc13-1-interacting domain and the Doc2alpha-Munc13-1 system is implicated in Ca(2+)-dependent synaptic vesicle exocytosis. The Munc13 family comprises the brain-specific Munc13-1, Munc13-2, and Munc13-3, and the non-neuronal Munc13-4. We previously showed that Munc13-4 is involved in Ca(2+)-dependent secretory lysosome exocytosis in mast cells, but the involvement of Doc2 in this process is not determined. In the present study, we found that Doc2alpha but not Doc2beta was endogenously expressed in the RBL-2H3 mast cell line. Doc2alpha colocalized with Munc13-4 on secretory lysosomes, and interacted with Munc13-4 through its two regions, the N terminus containing the Munc13-1-interacting domain and the C terminus containing the Ca(2+)-binding C2B domain. In RBL-2H3 cells, Ca(2+)-dependent secretory lysosome exocytosis was inhibited by expression of the Doc2alpha mutant lacking either of the Munc13-4-binding regions and the inhibition was suppressed by coexpression of Munc13-4. Knockdown of endogenous Doc2alpha also reduced Ca(2+)-dependent secretory lysosome exocytosis, which was rescued by re-expression of human Doc2alpha but not by its mutant that could not bind to Munc13-4. Moreover, Ca(2+)-dependent secretory lysosome exocytosis was severely reduced in bone marrow-derived mast cells from Doc2alpha knockout mice. These results suggest that the Doc2alpha-Muunc13-4 system regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.
Ayuko Sakane, Miyoshi Jun, Takai Yoshimi and Takuya Sasaki : Analysis on the Emerging Role of Rab3 GTPase-Activating Protein in Warburg Micro and Martsolf Syndrome., Methods in Enzymology, Vol.438, 131-139, 2008.
(Summary)
Evidence is accumulating that Rab3A plays a key role in neurotransmitter release and synaptic plasticity. Recently mutations in the catalytic subunit p130 and the noncatalytic subunit p150 of Rab3 GTPase-activating protein were found to cause Warburg Micro syndrome and Martsolf syndrome, respectively, both of which exhibit mental retardation. We have found that loss of p130 in mice results in inhibition of Ca2+-dependent glutamate release from cerebrocortical synaptosomes and alters short-term plasticity in the hippocampal CA1 region, probably through the accumulation of the GTP-bound form of Rab3A. Here, we describe the procedures for the measurement of the GTP-bound pool of Rab3A with pull-down assay using mouse brains and the biochemical method for the measurement of glutamate release from mouse synaptosomes.
Ayuko Sakane, Shigetsugu Hatakeyama and Takuya Sasaki : Involvement of Rabring7 in EGF receptor degradation as an E3 ligase., Biochemical and Biophysical Research Communications, Vol.357, No.4, 1058-1064, 2007.
(Summary)
Rab7, a member of the Rab family small G proteins, is involved in the late stage of the endocytic pathway. We previously identified a Rab7 target protein, Rabring7, which contains a RING finger domain at its C termini, but the precise role of Rabring7 remains unknown. In this study, we demonstrate using an in vitro ubiquitination assay with recombinant E1 and E2 proteins that Rabring7 has E3 ligase activity and that it preferentially reacts with Ubc4 and Ubc5 as its E2 proteins. Rabring7 ubiquitinated itself but not Rab7, and a mutation at Cys-229 in the RING finger domain (Rabring7C229S) completely diminished its E3 ligase activity. In the ligand-induced degradation of EGF receptor (EGFR), Rabring7 accelerated the degradation of EGFR, whereas Rabring7C229S inhibited the degradation induced by cCbl, another E3 ligase. These results suggest that Rabring7 is involved in the endocytic trafficking of EGFR through its E3 ligase activity.
(Keyword)
Cell Line / Endocytosis / Enzyme Activation / Humans / Intracellular Signaling Peptides and Proteins / Kidney / Protein Binding / Receptor, Epidermal Growth Factor / Ubiquitin-Protein Ligases
Ayuko Sakane, Shinji Manabe, Hiroyoshi Ishizaki, Miki Tanaka-Okamoto, Emi Kiyokage, Kazunori Toida, Takayuki Yoshida, Jun Miyoshi, Haruyuki Kamiya, Yoshimi Takai and Takuya Sasaki : Rab3 GTPase-activating protein regulates synaptic transmission and plasticity through the inactivation of Rab3., Proceedings of the National Academy of Sciences of the United States of America, Vol.103, No.26, 10029-10034, 2006.
(Summary)
Rab3A small G protein is a member of the Rab family and is most abundant in the brain, where it is localized on synaptic vesicles. Evidence is accumulating that Rab3A plays a key role in neurotransmitter release and synaptic plasticity. Rab3A cycles between the GDP-bound inactive and GTP-bound active forms, and this change in activity is associated with the trafficking cycle of synaptic vesicles at nerve terminals. Rab3 GTPase-activating protein (GAP) stimulates the GTPase activity of Rab3A and is expected to determine the timing of the dissociation of Rab3A from synaptic vesicles, which may be coupled with synaptic vesicle exocytosis. Rab3 GAP consists of two subunits: the catalytic subunit p130 and the noncatalytic subunit p150. Recently, mutations in p130 were found to cause Warburg Micro syndrome with severe mental retardation. Here, we generated p130-deficient mice and found that the GTP-bound form of Rab3A accumulated in the brain. Loss of p130 in mice resulted in inhibition of Ca(2+)-dependent glutamate release from cerebrocortical synaptosomes and altered short-term plasticity in the hippocampal CA1 region. Thus, Rab3 GAP regulates synaptic transmission and plasticity by limiting the amount of the GTP-bound form of Rab3A.
Kouichi Mizuno, Ayuko Sakane and Takuya Sasaki : Rabring7: A target protein for Rab7 small G protein., Methods in Enzymology, Vol.403, 687-696, 2005.
(Summary)
Rab7, a member of the Rab family of small G proteins, has been shown to regulate late endocytic traffic and lysosome biogenesis, but the exact roles and the mode of actions of Rab7 are still undetermined. Accumulating evidence suggests that each Rab protein has multiple target proteins and works together with them to coordinate the individual step of vesicle traffic. Rabring7 (Rab7-interacting ring finger protein) is a Rab7 target protein that has been isolated using a CytoTrap system. This protein shows no homology with RILP, which has been reported as another Rab7 target protein. Rabring7 is recruited efficiently to late endosome/lysosome by the GTP-bound form of Rab7. Exogenous expression of Rabring7 not only affects epidermal growth factor degradation but also induces the perinuclear aggregation of lysosomes and the increased acidity in the lysosomes. This chapter describes the procedures for the isolation of Rabring7 with a CytoTrap system, the analysis of the Rab7-Rabring7 interactions, and the properties of Rabring7.
(Keyword)
Animals / Cell Line / Cricetinae / Green Fluorescent Proteins / Humans / Immunoprecipitation / Protein Binding / rab GTP-Binding Proteins
Ayuko Sakane and Takuya Sasaki : Exocytotic Rab family small GTPase play crucial roles in synaptic and epithelial plasticity, Tanpakushitsu Kakusan Koso, Vol.53, No.16, 2130-2135, Dec. 2008.
(Keyword)
高次機能システム / 調節性エキソサイトーシス / Rabファミリー低分子量GTPase
(Link to Search Site for Scientific Articles)
● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21038597
Takuya Sasaki and Ayuko Sakane : Role of Rab Small G proteins in cellular morphogenesis., 札幌国際がんシンポジウム2010「メンブレントラフィックとがん」, Sapporo, Jun. 2010.
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Takuya Sasaki, Noriyuki Nishimura and Ayuko Sakane : Functions of Rab family small G proteins in regulated exocytosis., International Symposium on Membrane Traffic, Awaji, Nov. 2007.
Ayuko Sakane, 水口 賢司, 土屋 裕子 and Takuya Sasaki : Conformational plasticity of JRAB/MICAL-L2 provides ``law and order'' in collective cell migration, 第17回日本蛋白質科学会年会, Jun. 2017.
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Ayuko Sakane : 1分子の構造変化から観た細胞運動の制御機構, 第6回生命科学阿波踊りシンポジウム, Aug. 2015.
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松下 夏樹, 松下 佐知, Ayuko Sakane, Takuya Sasaki and 今村 健志 : Efficient gene-targeting in cultured cells by CRISPR/Cas9 system using non-integrating lentiviral vector, 第37回日本分子生物学会年会, Nov. 2014.
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Ayuko Sakane, 西村 将臣, 吉澤 信, 横田 秀夫 and Takuya Sasaki : The conformational plasticity of single molecule provides law and order in collective cell migration, 第87回日本生化学会大会, Oct. 2014.
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Ayuko Sakane, 西村 将臣, 横田 秀夫 and Takuya Sasaki : The conformational plasticity of JRAB provides law and order in collective cell migration, 第73回日本癌学会総会, Sep. 2014.
Ayuko Sakane and Takuya Sasaki : 動く細胞, ViEW2012ビジョン技術の実利用ワークショップ, Dec. 2012.
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Ayuko Sakane and Takuya Sasaki : 細胞接着・運動においてRab13-JRAB系が制御する小胞輸送とアクチン細胞骨格再編成, 第70回日本癌学会学術総会, Oct. 2011.
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Ayuko Sakane and Takuya Sasaki : A role of JRAB in epithelial junctional development: The cross-talk between vesicular trafficking and actin cytoskeleton in the submembrane plaque, 第63回日本細胞生物学会大会, Jun. 2011.
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Takuya Sasaki and Ayuko Sakane : Functions of Rab family small G proteins in neurite outgrowth., 第62回日本細胞生物学会大会シンポジウム, May 2010.
21.
Tabata Keisuke, Matsunaga Kohichi, Ayuko Sakane, Takuya Sasaki, Noda Takeshi and Yoshimori Tamotsu : The Rubicon family negatively regulates the endocytic pathway through the interactions with Rab7., The 62rd Annual Meeting of the Japan Society for Cell Biology, May 2010.
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Takuya Sasaki and Ayuko Sakane : Exocytotic Rab small G proteins regulate neuronal development and plasticity., BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会, Dec. 2008.
Ultra-sensitive and rapid detection of emerging and resurging viruses based on biomedical photonics of optical frequency comb (Project/Area Number: 22H00303 )
Analysis for conformational change of JRAB regulating collective cell migration; from molecular information to physiological role in mouse (Project/Area Number: 17K08636 )
Conformational plasticity of single molecule regulates actin cytoskeletal rearrangement leading to dynamics in higher structure and function of brain (Project/Area Number: 26460370 )