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Tokushima University > Research Clusters > Research Clusters (Specified) > 2001004 統合的がん研究創薬クラスター∼多段階発がん関連分子を標的とした中分子創薬の開発∼ >
Tokushima University > Research Clusters > Research Clusters (Registered) > 2004001 バイオマーカーの高感度ナノ分光検出・解析法の開拓と臨床・基礎医学応用 >
Tokushima University > Institute of Post-LED Photonics >
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Research Activity

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Field of Study

Biochemistry, Molecular Biology, Cell Biology

Subject of Study

細胞内小胞輸送ならびに細胞骨格の制御機構に関する研究 (細胞内シグナル伝達, 神経伝達物質放出, 細胞接着)

Book / Paper

Book:

1. Ayuko Sakane and Takuya Sasaki :
Roles of Rab family small G proteins in formation of the apical junctional complex in epithelial cells.,
Cell Polarity: Biological Role and Basic Mechanisms, 2014.
(DOI: 10.1007/978-3-319-14463-4_15)

Academic Paper (Judged Full Paper):

1. Shotaro Sakakibara, Kiyohito Mizutani, Ayumu Sugiura, Ayuko Sakane, Takuya Sasaki, Shigenobu Yonemura and Yoshimi Takai :
Afadin regulates actomyosin organization through αE-catenin at adherens junctions.,
The Journal of Cell Biology, Vol.219, No.5, 2020.
(DOI: 10.1083/jcb.201907079,   PubMed: 32227204)
2. Kazuhisa Miyake, Ayuko Sakane, Ikuko Sagawa, 富田 陽子, Jiro Kasahara and Takuya Sasaki :
Actin Cytoskeletal Reorganization Function of JRAB/MICAL-L2 Is Fine-tuned by Intramolecular Interaction between First LIM Zinc Finger and C-terminal Coiled-coil Domains,
Scientific Reports, Vol.9, No.1, 12794, 2019.
(DOI: 10.1038/s41598-019-49232-8,   PubMed: 31488862,   Elsevier: Scopus)
3. Ayuko Sakane, Shin Yoshizawa, Masaomi Nishimura, Yuko Tsuchiya, Natsuki Matsushita, Kazuhisa Miyake, Kazuki Horikawa, Issei Imoto, Chiharu Mizuguchi, Hiroyuki Saito, Takato Ueno, Sachi Matsushita, Hisashi Haga, Shinji Deguchi, Kenji Mizuguchi, Hideo Yokota and Takuya Sasaki :
Conformational plasticity of JRAB/MICAL-L2 provides "law and order" in collective cell migration.,
Molecular Biology of the Cell, Vol.27, No.20, 3095-3108, 2016.
(DOI: 10.1091/mbc.E16-05-0332,   PubMed: 27582384)
4. Ayuko Sakane, AA Abdallah, K Nakano, K Honda, T Kitamura, Issei Imoto, N Matsushita and Takuya Sasaki :
Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins.,
Genes to Cells, Vol.18, No.9, 810-822, 2013.
(Tokushima University Institutional Repository: 105908,   DOI: 10.1111/gtc.12078,   PubMed: 23890175,   Elsevier: Scopus)
5. Ayuko Sakane, Ahmed Alamir Mahmoud Abdallah, Kiyoshi Nakano, Kazufumi Honda, Wataru Ikeda, Yumiko Nishikawa, Mitsuru Matsumoto, Natsuki Matsushita, Toshio Kitamura and Takuya Sasaki :
Rab13 small G protein and junctional Rab13-binding protein (JRAB) orchestrate actin cytoskeletal organization during epithelial junctional development.,
The Journal of Biological Chemistry, Vol.287, No.51, 42455-42468, 2012.
(DOI: 10.1074/jbc.M112.383653,   PubMed: 23100251)
6. Kanchanamala Withanage, Kentaro Nakagawa, Mitsunobu Ikeda, Hidetake Kurihara, Takumi Kudo, Zeyu Yang, Ayuko Sakane, Takuya Sasaki and Yutaka Hata :
Expression of RASSF6 in kidney and the implication of RASSF6 and the Hippo pathway in the sorbitol-induced apoptosis in renal proximal tubular epithelial cells.,
The Journal of Biochemistry, Vol.152, No.1, 111-119, 2012.
(DOI: 10.1093/jb/mvs056,   PubMed: 22577168)
7. Keisuke Tabata, Kohichi Matsunaga, Ayuko Sakane, Takuya Sasaki, Takeshi Noda and Tamotsu Yoshimori :
Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain.,
Molecular Biology of the Cell, Vol.21, No.23, 4162-4173, 2010.
(DOI: 10.1091/mbc.E10-06-0495,   PubMed: 20943950)
8. Ayuko Sakane, Kazufumi Honda and Takuya Sasaki :
Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2.,
Molecular and Cellular Biology, Vol.30, No.4, 1077-1087, 2010.
(DOI: 10.1128/MCB.01067-09,   PubMed: 20008558)
9. Anita Szodorai, Yung-Hui Kuan, Silke Hunzelmann, Ulrike Engel, Ayuko Sakane, Takuya Sasaki, Yoshimi Takai, Joachim Kirsch, Ulrike Muller, Konrad Beyreuther, Scott Brady, Gerardo Morfini and Stefan Kins :
APP Anterograde Transport Requires Rab3A GTPase Activity for Assembly of the Transport Vesicle,
The Journal of Neuroscience, Vol.29, No.46, 14534-14544, 2009.
(DOI: 10.1523/JNEUROSCI.1546-09.2009,   PubMed: 19923287)
10. Hironori Higashio, Noriyuki Nishimura, Hiroyoshi Ishizaki, Jun Miyoshi, Satoshi Orita, Ayuko Sakane and Takuya Sasaki :
Doc2α and Munc13-4 Regulate Ca2+-Dependent Secretory Lysosome Exocytosis in Mast Cells.,
The Journal of Immunology, Vol.180, No.7, 4774-4784, 2008.
(PubMed: 18354201)
11. Ayuko Sakane, Miyoshi Jun, Takai Yoshimi and Takuya Sasaki :
Analysis on the Emerging Role of Rab3 GTPase-Activating Protein in Warburg Micro and Martsolf Syndrome.,
Methods in Enzymology, Vol.438, 131-139, 2008.
(DOI: 10.1016/S0076-6879(07)38009-9,   PubMed: 18413245,   Elsevier: Scopus)
12. Ayuko Sakane, Shigetsugu Hatakeyama and Takuya Sasaki :
Involvement of Rabring7 in EGF receptor degradation as an E3 ligase.,
Biochemical and Biophysical Research Communications, Vol.357, No.4, 1058-1064, 2007.
(DOI: 10.1016/j.bbrc.2007.04.052,   PubMed: 17462600)
13. Ayuko Sakane, Shinji Manabe, Hiroyoshi Ishizaki, Miki Tanaka-Okamoto, Emi Kiyokage, Kazunori Toida, Takayuki Yoshida, Jun Miyoshi, Haruyuki Kamiya, Yoshimi Takai and Takuya Sasaki :
Rab3 GTPase-activating protein regulates synaptic transmission and plasticity through the inactivation of Rab3.,
Proceedings of the National Academy of Sciences of the United States of America, Vol.103, No.26, 10029-10034, 2006.
(DOI: 10.1073/pnas.0600304103,   PubMed: 16782817)
14. Kouichi Mizuno, Ayuko Sakane and Takuya Sasaki :
Rabring7: A target protein for Rab7 small G protein.,
Methods in Enzymology, Vol.403, 687-696, 2005.
(DOI: 10.1016/S0076-6879(05)03059-4,   PubMed: 16473630)

Review, Commentary:

1. Ayuko Sakane and Takuya Sasaki :
Rab3Aと神経可塑性,
生体の科学, Vol.61, No.5, 426-427, 2010.
2. Ayuko Sakane and Takuya Sasaki :
RabファミリーSmall Gタンパク質による小胞輸送制御と高次生命機能,
細胞工学, Vol.28, No.12, 1247-1252, Nov. 2009.
3. Ayuko Sakane and Takuya Sasaki :
Exocytotic Rab family small GTPase play crucial roles in synaptic and epithelial plasticity,
Tanpakushitsu Kakusan Koso, Vol.53, No.16, 2130-2135, Dec. 2008.
(PubMed: 21038597,   CiNii: 40016364863)
4. Ayuko Sakane and Takuya Sasaki :
シナプス伝達と可塑性を支える小胞輸送 —Rab3A系を中心とした神経伝達物質放出の制御機構—,
実験医学, Vol.24, No.14, 2098-2103, Sep. 2006.
5. Ayuko Sakane and Takuya Sasaki :
神経可塑性とRab3A,
Clinical Neuroscience, Vol.24, No.7, 826, Jul. 2006.
6. Ayuko Sakane and Takuya Sasaki :
神経伝達物質放出の制御機構—開口分泌を制御するRab低分子量G蛋白質に注目して,
内分泌・糖尿病科, Vol.25, No.3, 200-205, 2006.
7. Ayuko Sakane and Takuya Sasaki :
神経伝達物質放出の制御分子群とCa2+チャネル,
医学のあゆみ 別冊 イオンチャネル最前線update 臓器でのイオンチャネルの働きと疾患, 197-202, Aug. 2005.

Proceeding of International Conference:

1. Takuya Sasaki and Ayuko Sakane :
Role of Rab Small G proteins in cellular morphogenesis.,
札幌国際がんシンポジウム2010「メンブレントラフィックとがん」, Sapporo, Jun. 2010.
2. Takuya Sasaki, Noriyuki Nishimura and Ayuko Sakane :
Functions of Rab family small G proteins in regulated exocytosis.,
International Symposium on Membrane Traffic, Awaji, Nov. 2007.

Proceeding of Domestic Conference:

1. 松下 夏樹, 松下 佐知, Ayuko Sakane, Takuya Sasaki and 今村 健志 :
Efficient gene-targeting in cultured cells by CRISPR/Cas9 system using non-integrating lentiviral vector,
第37回日本分子生物学会年会, Nov. 2014.
2. Ayuko Sakane, 西村 将臣, 吉澤 信, 横田 秀夫 and Takuya Sasaki :
The conformational plasticity of single molecule provides law and order in collective cell migration,
第87回日本生化学会大会, Oct. 2014.
3. Ayuko Sakane, 西村 将臣, 横田 秀夫 and Takuya Sasaki :
The conformational plasticity of JRAB provides law and order in collective cell migration,
第73回日本癌学会総会, Sep. 2014.
4. Ayuko Sakane, 西村 将臣, 吉澤 信, 横田 秀夫 and Takuya Sasaki :
集団的細胞運動においてRab13-JRAB系がつくりだす秩序と法則性,
第66回日本細胞生物学会, Jun. 2014.
5. Ayuko Sakane :
動く細胞,
ViEW2012ビジョン技術の実利用ワークショップ, Dec. 2012.
6. Ayuko Sakane and Takuya Sasaki :
細胞接着・運動においてRab13-JRAB系が制御する小胞輸送とアクチン細胞骨格再編成,
第70回日本癌学会学術総会, Oct. 2011.
7. Ayuko Sakane and Takuya Sasaki :
A role of JRAB in epithelial junctional development: The cross-talk between vesicular trafficking and actin cytoskeleton in the submembrane plaque,
第63回日本細胞生物学会大会, Jun. 2011.
8. Takuya Sasaki and Ayuko Sakane :
Functions of Rab family small G proteins in neurite outgrowth.,
第62回日本細胞生物学会大会シンポジウム, May 2010.
9. Tabata Keisuke, Matsunaga Kohichi, Ayuko Sakane, Takuya Sasaki, Noda Takeshi and Yoshimori Tamotsu :
The Rubicon family negatively regulates the endocytic pathway through the interactions with Rab7.,
The 62rd Annual Meeting of the Japan Society for Cell Biology, May 2010.
10. Takuya Sasaki and Ayuko Sakane :
Exocytotic Rab small G proteins regulate neuronal development and plasticity.,
BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会, Dec. 2008.
11. Takuya Sasaki, Noriyuki Nishimura and Ayuko Sakane :
高次細胞機能発現におけるエキソサイトーシス系Rabファミリー低分子量G蛋白質の作用機構,
第60回日本細胞生物学会大会, Jun. 2008.
12. Hironori Higashio, Noriyuki Nishimura, 石崎 宏好, 三好 淳, 織田 聡, Ayuko Sakane and Takuya Sasaki :
マスト細胞の調節性分泌におけるDoc2α-Munc13-4系の役割,
第30回日本分子生物学会年会・第80回日本生化学会大会合同大会, Dec. 2007.
13. Ayuko Sakane, 三好 淳, 田中 滋康, Toshiya Matsuzaki, Minoru Irahara, 石崎 宏好, 岡本 三紀, 高井 義美 and Takuya Sasaki :
Rab3低分子量G蛋白質ファミリーによる視床下部-下垂体-性腺系の制御,
第80回日本内分泌学会学術総会, Jun. 2007.
14. Hironori Higashio, Noriyuki Nishimura, 石崎 宏好, 三好 淳, Ayuko Sakane and Takuya Sasaki :
マスト細胞の調節性分泌におけるDoc2の役割,
第59回日本細胞生物学会, May 2007.
15. Ayuko Sakane, Shinji Manabe, 石崎 宏好, 岡本 三紀, 吉田 隆行, 三好 淳, 神谷 温之, 高井 義美 and Takuya Sasaki :
Rab3GAPによるシナプス伝達と可塑性の制御,
第28回日本分子生物学会年会, Dec. 2005.
16. Ayuko Sakane, 真鍋 進治, 石崎 宏好, 岡本 三紀, 吉田 隆行, 三好 淳, 神谷 温之 and 高井 義美 :
Rab3GAPによるシナプス伝達と可塑性の制御,
第28回日本分子生物学会年会, Dec. 2005.
17. Noriyuki Nishimura, 寺井 智也, Ayuko Sakane, 神田 郁乃 and Takuya Sasaki :
Rabファミリー低分子量G蛋白質による細胞極性・接着の制御機構,
第45回日本生化学会中国・四国支部例会, May 2004.
18. Shinya Morimoto, Noriyuki Nishimura, Akiko Kitamura, 品原 和加子, 山本 恭代, 寺井 智也, Ayuko Sakane, Shinji Manabe, Seiki Tashiro and Takuya Sasaki :
Rabファミリー低分子量G蛋白質によるタイトジャンクション膜蛋白質の小胞輸送の制御機構,
第62回日本癌学会総会, Sep. 2003.

Et cetera, Workshop:

1. Ayuko Sakane :
細胞接着形成を制御する細胞内小胞輸送とアクチン細胞骨格系のクロストーク機構,
第3回生命科学阿波踊りシンポジウム, Aug. 2011.
2. Ayuko Sakane :
細胞の形作りの分子機構の研究からがん転移・浸潤の病態を探る,
HBS 5教育部第20回月例交流セミナー, May 2011.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

  • 集団的細胞運動を制御するJRABの構造変化の階層的解析-情報から生体に至るまで- (Project/Area Number: 17K08636 )
  • Conformational plasticity of single molecule regulates actin cytoskeletal rearrangement leading to dynamics in higher structure and function of brain (Project/Area Number: 26460370 )
  • 集団的細胞運動を支える細胞内小胞輸送とアクチン細胞骨格再編成のクロストーク制御 (Project/Area Number: 25111723 )
  • The role of exocytosis in biological functions (Project/Area Number: 24790285 )
  • Synaptic formation and maturation regulated by membrane trafficking leading to higher function of the nervous system (Project/Area Number: 22790293 )
  • Regulation of membrane trafficking in synaptic formation and maturation. (Project/Area Number: 20890149 )
  • Search by Researcher Number (60509777)