Tokushima University / Educator and Researcher Directory --- Aki, Kensaku

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Academic Paper (Judged Full Paper):

1.	Kensaku Aki, 三輪詩佳, 佐藤瑞樹, 曽根淳美, Kazuyoshi Kawazoe and Eiji Hosoi : Secretion dynamics of cytotoxic granules in the cytotoxicity of the human NK-like cultured cells KHYG-1, Shikoku Acta Medica, Vol.76, No.3,4, 137-142, 2020. (Keyword) NK cell / KHYG-1 / cytotoxicity / granzyme B / IL-2 (Tokushima University Institutional Repository) ● Metadata: 115358 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1051693803346296448 (Tokushima University Institutional Repository: 115358, CiNii: 1051693803346296448)
2.	Kensaku Aki, 佐藤瑞樹, Atsumi Sone, Kazuyoshi Kawazoe and Eiji Hosoi : Evaluation of NK cell function using human NK-like cultured cell, KHYG-1, Shikoku Acta Medica, Vol.75, No.5.6, 165-170, 2019. (Summary) NK cells play an important role in the elimination of viral infection and tumors. In recent years, immune cell therapy using activated NK cells has attracted attention, and the search for activators and evaluation of NK cell activity have become important. We previously reported a positive correlation between CD56antigen expression, which is the major cell membrane antigen of human NK cells, and NK cell activity or cytotoxicity, and demonstrated that it is possible to evaluate NK cell function using the CD56 antigen as an index. This simple evaluation method is useful for functional evaluation of NK cells and the search for activators. However, it requires blood sampling and preparation of NK cells because it uses human NK cells. Therefore, in this study, we examined whether the CD56 antigen functions as an activation index using KHYG‐1 human NK-like cultured cells as a substitute for NK cells. As a result, the CD56antigen on the KHYG‐1cell membrane was increased in a concentration-dependent manner by IL‐2 stimulation, as was the cytotoxicity. This suggests that the CD56 antigen on the KHYG‐1 cell membrane can be used as an evaluation index of NK activity as in human NK cells. (Keyword) NK cell / KHYG-1 / CD56 antigen / IL-2 (Tokushima University Institutional Repository) ● Metadata: 114636 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050848249688556288 (Tokushima University Institutional Repository: 114636, CiNii: 1050848249688556288)
3.	Kensaku Aki, Azusa Izumi, Wataru Oboshi, Atsumi Sone, Masao Hirose and Eiji Hosoi : ABO genotyping of various hematopoietic cell lines to select model cells for research purposes, Human Immunology, 2019. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.humimm.2019.08.002 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.humimm.2019.08.002 (DOI: 10.1016/j.humimm.2019.08.002)
4.	Tomoki Tada, Kensaku Aki, Wataru Oboshi, Kazuyoshi Kawazoe, Toshiyuki Yasui and Eiji Hosoi : Evaluation of effects of various drugs on platelet functions using phorbol 12-myristate 13-acetate-induced megakaryocytic human erythroid leukemia cells, Drug Design, Development and Therapy, Vol.10, 3099-3107, 2016. (Summary) The hyperfunction and activation of platelets have been strongly implicated in the development and recurrence of arterial occlusive disease, and various antiplatelet drugs are used to treat and prevent such diseases. New antiplatelet drugs and many other drugs have been developed, but some drugs may have adverse effects on platelet functions. The aim of this study was to establish an evaluation method for evaluating the effect and adverse effect of various drugs on platelet functions. Human erythroid leukemia (HEL) cells were used after megakaryocytic differentiation with phorbol 12-myristate 13-acetate as an alternative to platelets. Drugs were evaluated by changes in intracellular Ca(2+) concentration ([Ca(2+)]i) mobilization in Fura2-loaded phorbol 12-myristate 13-acetate-induced HEL cells. Aspirin and cilostazol were selected as antiplatelet drugs and ibuprofen and sodium valproate as other drugs. There was a positive correlation between [Ca(2+)]i and platelet aggregation induced by thrombin. Aspirin (5.6-560 µM) and cilostazol (5-10 µM) significantly inhibited thrombin-induced increases in [Ca(2+)]i in a concentration-dependent manner. On the other hand, ibuprofen (8-200 µM) and sodium valproate (50-1,000 µg/mL) also significantly inhibited thrombin-induced increases in [Ca(2+)]i in a concentration-dependent manner. Furthermore, the interaction effects of the simultaneous combined use of aspirin and ibuprofen or sodium valproate were evaluated. When the inhibitory effect of aspirin was higher than that of ibuprofen, the effect of aspirin was reduced, whereas when the inhibitory effect of aspirin was lower than that of ibuprofen, the effect of ibuprofen was reduced. The combination of aspirin and sodium valproate synergistically inhibited thrombin-induced [Ca(2+)]i. It is possible to induce HEL cells to differentiate into megakaryocytes, which are a useful model for the study of platelet functions, and the quantification of the inhibition of thrombin-induced increases in [Ca(2+)]i is applicable to the evaluation of the effects of various drugs on platelets. (Keyword) Aspirin / Blood Platelets / Humans / Megakaryocytes / Phorbol Esters / Platelet Activation / Platelet Aggregation / Platelet Aggregation Inhibitors / Platelet Function Tests / Tetradecanoylphorbol Acetate / Tetrazoles (Tokushima University Institutional Repository) ● Metadata: 110054 (Link to Publication Site) ● Publication site (DOI): 10.2147/DDDT.S115910 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27713620 ● Summary page in Scopus @ Elsevier: 2-s2.0-84989182163 (Tokushima University Institutional Repository: 110054, DOI: 10.2147/DDDT.S115910, PubMed: 27713620, Elsevier: Scopus)
5.	Wataru Oboshi, Kensaku Aki, Tomoki Tada, Toru Watanabe, Nobuyasu Yukimasa, Ichiro Ueno, Ken Saito and Eiji Hosoi : Flow Cytometric Evaluation of Surface CD56 Expression on Activated Natural Killer Cells as Functional Marker., The Journal of Medical Investigation : JMI, Vol.63, No.3,4, 199-203, 2016. (Summary) Surface CD56 is the most important cell marker for defining NK cells. However, the relationship between the expression of surface CD56 and NK cell activity has not yet been elucidated in detail. Thirteen healthy volunteers were enrolled in the present study. Peripheral blood mononuclear cells (PBMCs) were stimulated with rIL-2 or rIL-12 (1, 10, 100 U/mL) for 18 h at 37°C. After incubation, surface CD56 expression on NK cells was evaluated using a flow cytometric analysis. A colorimetric-based lactate dehydrogenase (LDH) assay was used for experiments on cytotoxicity. IFN- mRNA gene expression was quantified by real-time PCR. The expression level of surface CD56 on NK cells, cytotoxicity, and IFN- mRNA gene expression were significantly increased by the rIL-2 and rIL-12 stimulations. In addition, a positive correlation was found between surface CD56 expression and cytotoxic activity or IFN- mRNA gene expression. We revealed that the quantification of surface CD56 expression was applicable to the evaluation of cytotoxicity and IFN- production in activated NK cells. These results suggest that the measurement of surface CD56 expression represent an easy and rapidly reproducible technique to evaluate the activated state of NK cells and monitor NK cell activity in immunotherapy. J. Med. Invest. 63: 199-203, August, 2016. (Tokushima University Institutional Repository) ● Metadata: 111181 (Link to Publication Site) ● Publication site (DOI): 10.2152/jmi.63.199 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27644558 ● Summary page in Scopus @ Elsevier: 2-s2.0-84987879967 (Tokushima University Institutional Repository: 111181, DOI: 10.2152/jmi.63.199, PubMed: 27644558, Elsevier: Scopus)
6.	Wataru Oboshi, Toru Watanabe, Nobuyasu Yukimasa, Ichiro Ueno, Kensaku Aki, Tomoki Tada and Eiji Hosoi : SNPs rs4656317 and rs12071048 located within an enhancer in FCGR3A are in strong linkage disequilibrium with rs396991 and influence NK cell-mediated ADCC by transcriptional regulation., Human Immunology, Vol.77, 997-1003, 2016. (Summary) CD16 receptors are mainly expresses on the surface of NK cells and mediate antibody-dependent cellular cytotoxicity (ADCC). The authors previously reported that NK cell-mediated ADCC is influenced by the single nucleotide polymorphism (SNP) rs396991 (T>G; F158V), and the structure and expression levels of CD16 differed among these genotypes. The authors examined haplotype frequency distributions among rs396991 and other SNPs, rs10917571 (G>T), rs4656317 (C>G), and rs12071048 (G>A), located in an enhancer of the FCGR3A gene. A total of 101 healthy Japanese were genotyped for the presence of these SNPs. The authors also measured ADCC activity, FCGR3A transcript levels, and surface CD16 expression on NK cells. We found that the regulatory SNPs (rSNPs) rs4656317 and rs12071048 were in strong linkage disequilibrium with rs396991. These two SNPs with major alleles had higher ADCC activity than those with minor alleles. In addition, FCGR3A transcript levels and surface CD16 expression levels were regulated by these SNPs. These findings suggest that NK cell-mediated ADCC could be influenced by transcriptional regulation of these rSNPs. These findings help to clarify our understanding of the linkage disequilibrium among functional SNPs in the FCGR3A gene, and provide a resource for investigating the roles of functional SNPs in NK cell-mediated ADCC. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.humimm.2016.06.012 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.humimm.2016.06.012 (DOI: 10.1016/j.humimm.2016.06.012)
7.	Wataru Oboshi, Toru Watanabe, Yuumi Matsuyama, Kobara Ayana, Nobuyasu Yukimasa, Ichiro Ueno, Kensaku Aki, Tomoki Tada and Eiji Hosoi : The influence of NK cell-mediated ADCC: Structure and expression of the CD16 molecule differ among FcRIIIa-V158F genotypes in healthy Japanese subjects, Human Immunology, Vol.77, No.2, 165-171, 2016. (Summary) NK cells express the CD16 (FcRIIIa) receptor, which mediates antibody-dependent cellular cytotoxicity (ADCC), on their cell surface. Therefore, ADCC activity may be influenced by qualitative or quantitative changes in the CD16 molecule on NK cells. Responses to NK cell-mediated ADCC have been shown to depend on single nucleotide polymorphisms (SNPs) at FcRIIIa amino acid position 158. However, a consensus has not yet been reached regarding differences in the structure and expression levels of the CD16 molecule among FcRIIIa-V158F genotypes, which have not yet been adequately investigated in healthy Japanese individuals. We herein examined the influence of the FcRIIIa polymorphism on ADCC, binding affinity of CD16 to the Fc region, FCGR3A gene expression, and cell-surface CD16 expression in healthy Japanese subjects. FcRIIIa-V158F genotyping was performed for 101 subjects. The results obtained showed that all parameters analyzed increased in the order of V/V>V/F>F/F and were significantly higher in V/V subjects than in F/F subjects. Moreover, a positive correlation was observed between ADCC activity and binding affinity, FCGR3A transcript levels, and surface CD16 expression levels. These results suggest that the structure and expression of the CD16 molecule differs among FcRIIIa-V158F genotypes, and the FcRIIIa-V158F polymorphism may be represent a haplotype with other SNPs in regulatory regions in Japanese subjects. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.humimm.2015.11.001 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26582002 ● Summary page in Scopus @ Elsevier: 2-s2.0-84958117862 (DOI: 10.1016/j.humimm.2015.11.001, PubMed: 26582002, Elsevier: Scopus)
8.	Kensaku Aki, Kazuyoshi Kawazoe, Azusa Izumi, Tomoki Tada, Kazuo Minakuchi and Eiji Hosoi : Direct Determination of ABO Blood Group Genotypes from Whole Blood Using PCR-Amplification of Specific Alleles Method, American Journal of BioScience, Vol.2, 49-55, 2014. (Link to Publication Site) ● Publication site (DOI): 10.11648/j.ajbio.20140202.15 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.11648/j.ajbio.20140202.15 (DOI: 10.11648/j.ajbio.20140202.15)
9.	Eiji Hosoi, Atsumi Sone and Kensaku Aki : Effects of lavender and grapefruit essential oil on the autonomic nervous system and immune system, アロマテラピー学雑誌, Vol.13, No.1, 29-40, 2013. (Keyword) aromatherapy / lavender oil / grapefruit oil / autonomic nervous system / immune cells / アロマテラピー / ラベンダー / グレープフルーツ / 自律神経 / 免疫細胞 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1521980706006785152 (CiNii: 1521980706006785152)
10.	Kensaku Aki, Azusa Izumi and Eiji Hosoi : The evaluation of histo-blood group ABO typing by flow cytometric and PCR Amplification of Specific Alleles analyses and their application in clinical laboratories, The Journal of Medical Investigation : JMI, Vol.59, No.1,2, 143-151, 2012. (Summary) ABO antigens are oligosaccharide antigens, and are widely distributed on red blood and tissue cells as well as in saliva and body fluid. Therefore, these antigens are important not only for blood transfusion, but also for tissue cell and organ transplantations. Also, blood, hair, and seminal fluid are important sources of evidence at crime scenes, and these antigens are some of the most important markers for personal identification in forensic investigations. Here, we describe the development and use of quantitative analysis of A, B, and H antigens on red blood cells by employing flow cytometric analysis and the ABO genotyping method based on PCR-amplification of specific alleles (PASA) within DNA, especially from blood and saliva. In this study, flow cytometric analysis could be used to compare the differences between the expression of A and/or B and H antigens on red blood cells with various phenotypes, and the PASA method was able to determine the genotype of the type cisA(2)B(3) pedigree using only DNA extracted from saliva. These analysis methods are simple and useful for judging the ABO blood group system and genotyping, and are used widely throughout research and clinical laboratories and forensic fields. (Keyword) ABO Blood-Group System / Alleles / Antigens, Bacterial / Biometric Identification / Flow Cytometry / Forensic Pathology / Histocompatibility Testing / Humans / Polymerase Chain Reaction (Tokushima University Institutional Repository) ● Metadata: 106016 (Link to Publication Site) ● Publication site (DOI): 10.2152/jmi.59.143 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22450003 ● Search Scopus @ Elsevier (PMID): 22450003 ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.59.143 (Tokushima University Institutional Repository: 106016, DOI: 10.2152/jmi.59.143, PubMed: 22450003)
11.	Kensaku Aki, 相原美奈子, 森内貴子, 森美和 and Eiji Hosoi : Preparation method for a specific anti-H lectin isolated from goya seeds and its application in clinical laboratories, Shikoku Acta Medica, Vol.67, No.5, 6, 247-252, 2011. (Summary) After the discovery of the phenomenon of aggregation of red blood cells with castor beanextract by Peter Hermann Stillmark in1888, various plant hemagglutinins were discovered fromleguminous plant seed extracts. These plant hemagglutinins were called"lectins"by William C.Boyd et al . Lectins are sugar-binding proteins that are highly specific for their sugar moieties,physiological activity, and hemagglutinating activity. In particular, lectins displaying blood-groupspecificity are important for blood-group typing and antigen recognition. In recent years, Ulexlectin(anti-H lectin)extracted from the Ulex europaeus seed was used to subdivide blood of typeO, and the subgroup of ABO, but Ulex europaeus is now difficult to obtain, so it is necessary to buyexpensive reagents.On the other hand, Momordica charantias (goya)seed extract(goya lectin)was recentlyrevealed to show higher anti-H activity than Ulex lectin. Goya is readily available and inexpensiveto obtain. Therefore, we examined a simple method for the preparation of goya lectin.As a result, we were able to establish a simple method for preparing goya lectin isolated fromgoya seeds, and confirm its stability against long-term storage and temperature and usefulness asan anti-H lectin. We expect this method to be used in clinical practice in the future. (Keyword) momordica charantias (goya) seeds / plant hemagglutinins / anti-H lectin / ABO blood typing / clinical laboratories (Tokushima University Institutional Repository) ● Metadata: 85231 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050845762393992320 (Tokushima University Institutional Repository: 85231, CiNii: 1050845762393992320)

Proceeding of International Conference:

1.	Hiroto Nishio, Yuko Narusawa, Kensaku Aki, Motoki Sugasaki, Takayuki Nakao, Takeshi Oya and Michiko Yamashita : Efficacy of Aether AIHema in Teaching Clinical Hematology to Undergraduate Medical Laboratory Science Students, ASCPaLM 2024, Seoul, Sep. 2024.

Proceeding of Domestic Conference:

1.	上井麻央, 吉良瑠里子 and Kensaku Aki : IL-15の NK 様培養細胞 KHYG-1活性に及ぼす影響, 第47回徳島県医学検査学会, Dec. 2023.
2.	古賀鈴奈, 吉田菜々花, IWAGAKI Yuma, Itsuro Endo, Kensaku Aki and Eiji Hosoi : 漢方薬の NK 様培養細胞 KHYG-1活性に及ぼす影響 - IFN-γ-, 第46回徳島県医学検査学会, Dec. 2022.
3.	吉田菜々花, 古賀鈴奈, IWAGAKI Yuma, Itsuro Endo, Kensaku Aki and Eiji Hosoi : 漢方薬の NK 様培養細胞 KHYG-1活性に及ぼす影響 - グランザイムB -, 第46回徳島県医学検査学会, Dec. 2022.
4.	吉良瑠里子, IWAGAKI Yuma, Itsuro Endo, Kensaku Aki and Eiji Hosoi : 血液型遺伝子プロモーター領域におけるメチル化と H抗原発現および HmRNA 発現量に関する検討, 第46回徳島県医学検査学会, Dec. 2022.
5.	野中蓮, IWAGAKI Yuma, Kensaku Aki and Eiji Hosoi : ヒト慢性骨髄性白血病由来 K562細胞を用いた赤血球系への分化誘導の検討, 第46回徳島県医学検査学会, Dec. 2022.
6.	大塚理央, 岩垣侑真, 豊田剛志, 曽根淳美, Toshiyuki Yasui, Kensaku Aki and Eiji Hosoi : ヒト血小板活性化における基礎的検討, 第45回徳島県医学検査学会, Dec. 2021.
7.	宮武大輔, 岩垣侑真, 豊田剛志, 曽根淳美, Itsuro Endo, Kensaku Aki and Eiji Hosoi : 血液型遺伝子プロモーター領域におけるメチル化とH抗原発現およびHmRNA発現量に関する検討, 第45回徳島県医学検査学会, Dec. 2021.
8.	山元美礼, 岩垣侑真, 豊田剛志, 曽根淳美, Itsuro Endo, Kensaku Aki and Eiji Hosoi : 漢方薬のNK様培養細胞KHYG-1活性に及ぼす影響, 第45回徳島県医学検査学会, Dec. 2021.
9.	海野仁希, 豊田剛志, Atsumi Sone, Kensaku Aki and Eiji Hosoi : 血液型遺伝子プロモーター領域におけるメチル化の検討, 第44回徳島県医学検査学会, Dec. 2020.
10.	岩垣さくら, 豊田剛志, Atsumi Sone, Kensaku Aki and Eiji Hosoi : 血小板活性化マーカー(CD62P)を指標とした血小板活性評価法の検討, 第44回徳島県医学検査学会, Dec. 2020.
11.	鈴鹿比奈子, Yuma Iwagaki, 豊田剛志, Atsumi Sone, Kensaku Aki and Eiji Hosoi : NK様培養細胞KHYG-1の活性化マーカーの検討 - 活性化レセプター(CD314) -, 第44回徳島県医学検査学会, Dec. 2020.
12.	Yuma Iwagaki, Hinako Suzuka, TOYODA Tsuyoshi, Atsumi Sone, Kensaku Aki and Eiji Hosoi : NK様培養細胞KHYG-1の活性化マーカーの検討 ―グランザイムおよびパーフォリン―, 第44回徳島県医学検査学会, Dec. 2020.
13.	蔵根理貴, 畑山晶, Atsumi Sone, Kensaku Aki and Eiji Hosoi : NK様培養細胞KHYG-1の活性化マーカーの検討, 第43回徳島県医学検査学会, Dec. 2019.
14.	畑山晶, 蔵根理貴, Atsumi Sone, Kensaku Aki and Eiji Hosoi : NK様培養細胞KHYG-1を用いた黄耆の NK細胞障害活性評価, 第43回徳島県医学検査学会, Dec. 2019.
15.	豊田剛志, 西村真智子, Atsumi Sone, Kensaku Aki and Eiji Hosoi : 血液培養細胞株HELの分化誘導に伴う細胞内シクロオキシゲナーゼ(COX)mRNA発現の基礎的研究, 第43回徳島県医学検査学会, Dec. 2019.
16.	西村真智子, 豊田剛志, Atsumi Sone, Kensaku Aki and Eiji Hosoi : 巨核球系細胞株CMK86の分化誘導に伴う細胞内シクロオキシゲナーゼ(COX)mRNA発現に関する検討, 第43回徳島県医学検査学会, Dec. 2019.
17.	崎山千尋, 大塚百華, Utaka Miwa, Atsumi Sone, Kensaku Aki and Eiji Hosoi : NK様培養細胞KHYG-1の活性化マーカーの検討, 第42回徳島県医学検査学会, Dec. 2018.
18.	山口結芽, Utaka Miwa, Atsumi Sone, Kensaku Aki and Eiji Hosoi : 巨核球系細胞株CMK86分化誘導に伴う細胞内シクロオキシゲナーゼ(COX)mRNA発現の基礎的研究, 第42回徳島県医学検査学会, Dec. 2018.
19.	大塚百華, 崎山千尋, Utaka Miwa, Atsumi Sone, Kensaku Aki and Eiji Hosoi : NK様培養細胞KHYG-1を用いた十全大補湯のNK細胞活性評価, 第42回徳島県医学検査学会, Dec. 2018.
20.	Utaka Miwa, Atsumi Sone, Kensaku Aki and Eiji Hosoi : 補中益気湯によるNK様培養細胞KHYG-1の細胞傷害活性評価, 第13回日本臨床検査学教育学会学術大会, Aug. 2018.
21.	清水小波, 中木竜馬, Utaka Miwa, Atsumi Sone, Kensaku Aki and Eiji Hosoi : 巨核球系細胞を血小板モデル細胞として用いた薬剤評価法の検討 - 巨核球系細胞株:CMK11-5 -, 第41回徳島県医学検査学会, Dec. 2017.
22.	中木竜馬, 清水小波, Utaka Miwa, Atsumi Sone, Kensaku Aki and Eiji Hosoi : 巨核球系細胞を血小板モデル細胞として用いた薬剤評価法の検討 - 巨核球系細胞株:CMK86 -, 第41回徳島県医学検査学会, Dec. 2017.
23.	Yuka Ikeda, Shuji Kuwahara, Utaka Miwa, Atsumi Sone, Kensaku Aki and Eiji Hosoi : NK細胞様T細胞株MTAを用いた NKT細胞活性評価の検討, 第41回徳島県医学検査学会, Dec. 2017.
24.	Shuji Kuwahara, Yuka Ikeda, Utaka Miwa, Atsumi Sone, Kensaku Aki and Eiji Hosoi : NK様細胞KHYG-1を用いた細胞傷害活性評価, 第41回徳島県医学検査学会, Dec. 2017.
25.	河内千佳, 三輪詩佳, 多田智紀, 植木春香, 川添和義, Kensaku Aki and Eiji Hosoi : 血小板表面抗原解析法を用いた血小板機能に対する薬剤評価法の検討, 第40回徳島県医学検査学会, Dec. 2016.
26.	Utaka Miwa, Chika Kohchi, Tomoki Tada, Wataru Ohboshi, Mizuki Satoh, 川添和義, Kensaku Aki and Eiji Hosoi : NK様細胞KHYG-1を用いた細胞傷害活性の検討, 第40回徳島県医学検査学会, Dec. 2016.
27.	Mami Yamamoto, Tomoki Tada, Wataru Ohboshi, Kensaku Aki and Eiji Hosoi : H遺伝子プローモーター領域におけるメチル化の解析, 第40回徳島県医学検査学会, Dec. 2016.
28.	上川千春, 山本真美, 多田智紀, 大星航, Kensaku Aki and Eiji Hosoi : ABO遺伝子プロモーター領域におけるメチル化が抗原発現に及ぼす影響, 第39回徳島県医学検査学会, Dec. 2015.
29.	酒井美由希, 小林沙綾, 多田智紀, 大星航, 植木春香, Kazuyoshi Kawazoe, Kensaku Aki and Eiji Hosoi : 血小板モデル細胞を用いた各種薬剤併用における薬剤効果·作用の評価, 第39回徳島県医学検査学会, Dec. 2015.
30.	小林沙綾, 酒井美由希, 多田智紀, 大星航, 佐藤瑞樹, Kazuyoshi Kawazoe, Kensaku Aki and Eiji Hosoi : NK様細胞KHYG-1を用いたNK細胞活性評価指標の検討, 第39回徳島県医学検査学会, Dec. 2015.
31.	多田智紀, Kensaku Aki, 大星航, Kazuyoshi Kawazoe, 植木春香, 川添和義 and Eiji Hosoi : 血小板モデル細胞を用いた各種薬剤評価の検討, 第10回日本臨床検査学教育学会学術大会, Aug. 2015.
32.	大星航, 多田智紀, Kensaku Aki and Eiji Hosoi : NK細胞表面抗原発現解析による新たなNK細胞活性評価法の確立(第3報), 日本輸血・細胞治療学会, May 2015.
33.	岡本拓也, 石倉美月, 多田智紀, 大星航, Kensaku Aki and Eiji Hosoi : FTAカード血液サンプルを用いたABO遺伝子型判定の検討, 第38回徳島県医学検査学会, Dec. 2014.
34.	石倉美月, 岡本拓也, 多田智紀, 大星航, Kensaku Aki and Eiji Hosoi : MSP法を用いたABO遺伝子のプロモーター領域におけるメチル化の検討, 第38回徳島県医学検査学会, Dec. 2014.
35.	後藤里香, 原田啓, 多田智紀, 大星航, 佐藤瑞樹, Kazuyoshi Kawazoe, Kensaku Aki and Eiji Hosoi : NK様細胞KHYG-1を用いたNK細胞活性評価の検討, 第38回徳島県医学検査学会, Dec. 2014.
36.	原田啓, 後藤里香, 多田智紀, 大星航, 植木春香, Kazuyoshi Kawazoe, Kensaku Aki and Eiji Hosoi : 巨核球系培養細胞CMKを用いた抗血小板薬の薬剤評価法の検討, 第38回徳島県医学検査学会, Dec. 2014.
37.	大星航, 多田智紀, Kensaku Aki and Eiji Hosoi : NK細胞活性化におけるCD16およびCD56発現の変化とその評価, 2014 感染・免疫クラスター・ミニリトリート[徳島大学大学院ヘルスバイオサイエンス研究部], Oct. 2014.
38.	多田智紀, 大星航, Kensaku Aki and Eiji Hosoi : 巨核球系培養細胞を用いた血小板機能におよぼす薬剤評価法の検討, 2014 感染・免疫クラスター・ミニリトリート[徳島大学大学院ヘルスバイオサイエンス研究部], Oct. 2014.
39.	多田智紀, 大星航, Kensaku Aki, Kazuyoshi Kawazoe and Eiji Hosoi : 巨核球系培養細胞を用いた薬剤評価の検討, 第9回日本臨床検査学教育学会学術大会, Aug. 2014.
40.	Wataru Ohboshi, Tomoki Tada, Kensaku Aki and Eiji Hosoi : NK細胞表面抗原発現解析による新たなNK細胞活性評価法の確立, 第62回日本輸血・細胞治療学会, May 2014.
41.	牧原汐里, 宮﨑渚, 多田智紀, 大星航, 松本直貴, Kazuyoshi Kawazoe, Kazuo Minakuchi, Kensaku Aki and Eiji Hosoi : 巨核球系分化誘導細胞を用いた抗血小板薬の評価, 第37回徳島県医学検査学会, Dec. 2013.
42.	酒井絵理, 福田翔太郎, 多田智紀, Kensaku Aki and Eiji Hosoi : ABO遺伝子のプロモーター領域におけるメチル化の検討∼融解曲線を用いた解析∼, 第36回徳島県医学検査学会, Dec. 2013.
43.	福田翔太郎, 酒井絵理, 多田智紀, Kensaku Aki and Eiji Hosoi : ABO遺伝子のプロモーター領域におけるメチル化の検討∼MSP法を用いた解析∼, 第37回徳島県医学検査学会, Dec. 2013.
44.	宮﨑渚, 牧原汐里, 多田智紀, 大星航, Kensaku Aki and Eiji Hosoi : ストレス状態におけるNK細胞への影響, 第37 回徳島県医学検査学会, Dec. 2013.
45.	Naoki Matsumoto, Tomoki Tada, Kensaku Aki, Wataru Ohboshi, 廣瀬政雄, Naoto Okada, Kazuyoshi Kawazoe, Eiji Hosoi and Kazuo Minakuchi : ヒト巨核球系細胞株CMKを用いた血小板機能に及ぼす各種薬剤評価の検討, 第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, Oct. 2013.
46.	Kensaku Aki, 多田智紀, 大星航, 廣瀬政雄 and Eiji Hosoi : 血液培養細胞株34 種類のABO 血液型に関する解析, 第58回日本輸血・細胞治療学会中四国支部例会, Sep. 2013.
47.	多田智紀, 松本直貴, Kensaku Aki, 大星航, Kazuyoshi Kawazoe, Kazuo Minakuchi and Eiji Hosoi : 巨核球系培養細胞を用いた抗血小板薬アスピリンの薬剤評価の検討, 第8回日本臨床検査学教育学会学術大会, Aug. 2013.
48.	Kensaku Aki, Eiji Hosoi and Tomoki Tada : ABO血液型遺伝子解析法により検出された稀な対立遺伝子, 第61回日本輸血・細胞治療学会, May 2013.
49.	堀家由貴, 谷井菜緒子, 多田智紀, 泉あずさ, 松本直貴, Kazuyoshi Kawazoe, Kazuo Minakuchi, Kensaku Aki and Eiji Hosoi : 巨核球系培養細胞を用いた薬剤評価の検討, 第36回徳島県医学検査学会, Dec. 2012.
50.	谷井菜緒子, 堀家由貴, 泉あずさ, 多田智紀, 松本直貴, Kazuyoshi Kawazoe, Kazuo Minakuchi, Kensaku Aki and Eiji Hosoi : 長期ストレス状態における免疫系への影響, 第36回徳島県医学検査学会, Dec. 2012.
51.	米田直美, 小倉有紀, 泉あずさ, 多田智紀, Kensaku Aki and Eiji Hosoi : ゴーヤ種子由来抗Hレクチンの生化学的解析, 第36回徳島県医学検査学会, Dec. 2012.
52.	小倉有紀, 米田直美, 泉あずさ, 多田智紀, 廣瀬政雄, Kensaku Aki and Eiji Hosoi : 各種血液培養細胞株のABO血液型に関する検討, 第36回徳島県医学検査学会, Dec. 2012.
53.	泉あずさ, 小倉有紀, Kensaku Aki, 多田智紀, 廣瀬政雄 and Eiji Hosoi : 各種血液培養細胞株におけるABO血液型genotypeの確定, 第57回日本輸血・細胞治療学会中国四国地方会, Sep. 2012.
54.	倍味宏行, 岸田真季, 山本歩実, Kensaku Aki, 泉あずさ, 足立麻衣, 曽根淳美 and Eiji Hosoi : ABO血液型遺伝子のExon6,7領域の各塩基置換におけるPASA法を用いた解析, 第35回徳島県医学検査学会, Dec. 2011.
55.	岸田真季, 倍味宏行, 山本歩実, 曽根淳美, 足立麻衣, 泉あずさ, Kensaku Aki, Naoki Matsumoto, Kazuyoshi Kawazoe, Kazuo Minakuchi and Eiji Hosoi : 精油による自律神経系及び免疫系への影響, 第35回徳島県医学検査学会, Dec. 2011.
56.	山本歩実, 岸田真季, 倍味宏行, 足立麻衣, 曽根淳美, 泉あずさ, Kensaku Aki, 松本直貴, Kazuyoshi Kawazoe, Kazuo Minakuchi and Eiji Hosoi : 血小板機能に及ぼす各種薬剤作用の血液培養細胞を用いた解析, 第35回徳島県医学検査学会, Dec. 2011.
57.	足立麻衣, Kensaku Aki, 曽根淳美, 泉あずさ and Eiji Hosoi : 血液培養細胞を用いた血小板機能に及ぼす各種薬剤作用の解析, 第44回中国四国医学検査学会, Nov. 2011.
58.	曽根淳美, Kensaku Aki, 泉あずさ, 足立麻衣 and Eiji Hosoi : ABO血液型別にみた血清IgG型抗A/B抗体価の検討, 第44回中国四国医学検査学会, Nov. 2011.
59.	Kensaku Aki, 泉あずさ, 足立麻衣, 曽根淳美 and Eiji Hosoi : 唾液抽出DNAを用いたABO血液型遺伝子解析法の確立, 第44回中国四国医学検査学会, Nov. 2011.
60.	Kensaku Aki, 泉あずさ, 足立麻衣, 曽根淳美 and Eiji Hosoi : 血液サンプルからの直接PCR法によるABO血液型遺伝子解析の検討, 第56回日本輸血・細胞治療学会, Sep. 2011.
61.	泉あずさ, 福良歌奈恵, Kensaku Aki, 足立麻衣, 曽根淳美 and Eiji Hosoi : Direct PCRキットによるPASA法でのABO遺伝子解析, 第34回徳島県医学検査学会, Dec. 2010.
62.	猪井紅美子, 平田有紀奈, Kensaku Aki, 曽根淳美, 足立麻衣, Eiji Hosoi, Kazuyoshi Kawazoe and Kazuo Minakuchi : ストレス条件下における免疫パラメータの変動, 第34回徳島県医学検査学会, Dec. 2010.
63.	福良歌奈恵, 泉あずさ, Kensaku Aki, 足立麻衣, 曽根淳美 and Eiji Hosoi : 非侵襲的サンプルからの抽出DNAを用いたPASA法でのABO遺伝子解析, 第34回徳島県医学検査学会, Dec. 2010.
64.	平田有紀奈, 猪井紅美子, Kensaku Aki, 足立麻衣, 曽根淳美, Eiji Hosoi, 谷口友紀, Kazuyoshi Kawazoe and Kazuo Minakuchi : 血小板モデル細胞を用いた薬剤評価測定系の確立, 第34回徳島県医学検査学会, Dec. 2010.
65.	仁木朋加, 足立麻衣, Kensaku Aki, Eiji Hosoi, 真部裕一, Kazuyoshi Kawazoe and Kazuo Minakuchi : ヒト血小板機能に及ぼすバルプロ酸Naによる抑制機構の検討2 ∼カルモジュリン(CaM)系への作用∼, 第33回徳島県医学検査学会, Dec. 2009.
66.	足立麻衣, 仁木朋加, Kensaku Aki, Eiji Hosoi, 真部裕一, Kazuyoshi Kawazoe and Kazuo Minakuchi : ヒト血小板機能に及ぼすバルプロ酸Naによる抑制機構の検討1∼プロテインキナーゼC(PKC)系への作用∼, 第33回徳島県医学検査学会, Dec. 2009.
67.	藤川貴弘, 曽根淳美, Kensaku Aki and Eiji Hosoi : ABO血液型の遺伝子解析 ∼プライマーの設計変更による非特異的バンドの抑制∼, 第33回徳島県医学検査学会, Dec. 2009.
68.	曽根淳美, 藤川貴弘, Kensaku Aki and Eiji Hosoi : 各血液型におけるIgG型抗A / B抗体価について, 第33回徳島県医学検査学会, Dec. 2009.
69.	真部裕一, Kensaku Aki, Kazuyoshi Kawazoe, Kazuo Minakuchi and Eiji Hosoi : バルプロ酸Naのヒト血小板機能に対する抑制作用, 第54回日本輸血·細胞治療学会中国·四国地方会, Nov. 2009.
70.	森内貴子, Kensaku Aki, 小林憲司 and Eiji Hosoi : ゴーヤ種子由来抗Hレクチンの安定性とその特異性, 第32回徳島県医学検査学会, Dec. 2008.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

Establishment of evaluation method of drugs using a megakaryocytic cultured cells. (Project/Area Number: 26670282 )
Search by Researcher Number (70646398)

Associate Professor : Aki, Kensaku

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)