Tokushima University / Educator and Researcher Directory --- Baba, Otto

Field of Study

○	Oral Anatomy
○	Oral Anatomy
○	Cell Physiology

Subject of Study

○	Dentin Formation (dentin, dentin protein, odontoblast, dental pulp)
○	Formation/Regeneration of Periodontium (alveolar bone, periodontal ligament, cementum, osteoblast, dentin proein)
○	metabolism of glycogen (glycogen, antibody, immunohistochemisry)

Book / Paper

Book:

1.	Otto Baba : 6, Ishiyaku Publishers,Inc., Tokyo, Feb. 2022. (Keyword) 頭頸部の動脈 / 頭頸部の静脈
2.	Otto Baba and 落合知子 : 医歯薬学系博物館事典, 雄山閣, Tokyo, May 2021.
3.	Otto Baba : 口腔解剖学第2版, Ishiyaku Publishers,Inc., Tokyo, Feb. 2018. (Keyword) 頭頸部の動脈 / 頭頸部の静脈

Academic Paper (Judged Full Paper):

1.	Tsuyoshi Morita, Shin Matsumoto and Otto Baba : Expression of secretory calcium-binding phosphoprotein (scpp) genes in medaka during the formation and replacement of pharyngeal teeth, BMC Oral Health, Vol.23, No.1, 744, 2023. (Summary) Analyses of tooth families and tooth-forming units in medaka with regard to tooth replacement cycles and the localization of odontogenic stem cell niches in the pharyngeal dentition clearly indicate that continuous tooth replacement is maintained. The secretory calcium-binding phosphoprotein (scpp) gene cluster is involved in the formation of mineralized tissues, such as dental and bone tissues, and the genes encoding multiple SCPPs are conserved in fish, amphibians, reptiles, and mammals. In the present study, we examined the expression patterns of several scpp genes in the pharyngeal teeth of medaka to elucidate their roles during tooth formation and replacement. Himedaka (Japanese medaka, Oryzias latipes) of both sexes (body length: 28 to 33 mm) were used in this study. Real-time quantitative reverse transcription-polymerase chain reaction (PCR) (qPCR) data were evaluated using one-way analysis of variance for multi-group comparisons, and the significance of differences was determined by Tukey's comparison test. The expression of scpp genes was examined using in situ hybridization (ISH) with a digoxigenin-labeled, single-stranded antisense probe. qPCR results showed that several scpp genes were strongly expressed in pharyngeal tissues. ISH analysis revealed specific expression of scpp1, scpp5, and sparc in tooth germ, and scpp5 was continually expressed in the odontoblasts of teeth attached to pedicles, but not in the osteoblasts of pedicles. In addition, many scpp genes were expressed in inner dental epithelium (ide), but not in odontoblasts, and scpp2 consistently showed epithelial-specific expression in the functional teeth. Taken together, these data indicate that specific expression of scpp2 and scpp5 may play a critical role in pharyngeal tooth formation in medaka. We characterized changes in the expression patterns of scpp genes in medaka during the formation and replacement of pharyngeal teeth. (Keyword) Humans / Animals / Oryzias / Calcium / Phosphoproteins / Odontogenesis / Bone and Bones / Mammals (Tokushima University Institutional Repository) ● Metadata: 118642 (Link to Publication Site) ● Publication site (DOI): 10.1186/s12903-023-03498-7 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37821862 ● Search Scopus @ Elsevier (PMID): 37821862 ● Search Scopus @ Elsevier (DOI): 10.1186/s12903-023-03498-7 (Tokushima University Institutional Repository: 118642, DOI: 10.1186/s12903-023-03498-7, PubMed: 37821862)
2.	Katsuhiko Ono, Hitoshi Gotoh, Tadashi Nomura, Tsuyoshi Morita, Otto Baba, Mami Matsumoto, Sei Saitoh and Nobuhiko Ohno : Ultrastructural characteristics of oligodendrocyte precursor cells in the early postnatal mouse optic nerve observed by serial block-face scanning electron microscopy, PLoS ONE, Vol.17, No.12, 1-18, 2022. (Summary) Oligodendrocyte precursor cells (OPC) arise from restricted regions of the central nervous system (CNS) and differentiate into myelin-forming cells after migration, but their ultrastructural characteristics have not been fully elucidated. This study examined the three-dimensional ultrastructure of OPCs in comparison with other glial cells in the early postnatal optic nerve by serial block-face scanning electron microscopy. We examined 70 putative OPCs (pOPC) that were distinct from other glial cells according to established morphological criteria. The pOPCs were unipolar in shape with relatively few processes, and their Golgi apparatus were localized in the perinuclear region with a single cisterna. Astrocytes abundant in the optic nerve were distinct from pOPCs and had a greater number of processes and more complicated Golgi apparatus morphology. All pOPCs and astrocytes contained a pair of centrioles (basal bodies). Among them, 45% of pOPCs extended a short cilium, and 20% of pOPCs had centrioles accompanied by vesicles, whereas all astrocytes with basal bodies had cilia with invaginated ciliary pockets. These results suggest that the fine structures of pOPCs during the developing and immature stages may account for their distinct behavior. Additionally, the vesicular transport of the centrioles, along with a short cilium length, suggests active ciliogenesis in pOPCs. (Keyword) Mice / Animals / Oligodendrocyte Precursor Cells / Microscopy, Electron, Scanning / Optic Nerve / Eye / Centrioles / Antioxidants (Tokushima University Institutional Repository) ● Metadata: 117793 (Link to Publication Site) ● Publication site (DOI): 10.1371/journal.pone.0278118 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36454994 ● Search Scopus @ Elsevier (PMID): 36454994 ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0278118 (Tokushima University Institutional Repository: 117793, DOI: 10.1371/journal.pone.0278118, PubMed: 36454994)
3.	Arief Waskitho, Yumiko Yamamoto, S Raman, Fumiya Kano, Huijiao Yan, R Raju, S Afroz, Tsuyoshi Morita, Daisuke Ikutame, Kazuo Okura, Masamitsu Ohshima, Akihito Yamamoto, Otto Baba and Yoshizo Matsuka : Peripherally Administered Botulinum Toxin Type A Localizes Bilaterally in Trigeminal Ganglia of Animal Model, Toxins, Vol.13, No.10, 704, 2021. (Tokushima University Institutional Repository) ● Metadata: 116383 (Link to Publication Site) ● Publication site (DOI): 10.3390/toxins13100704 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3390/toxins13100704 (Tokushima University Institutional Repository: 116383, DOI: 10.3390/toxins13100704)
4.	Huijiao Yan, Masamitsu Ohshima, Raju Raju, S Raman, Kazumitsu Sekine, Arief Waskitho, Miho Inoue, Masahisa Inoue, Otto Baba, Tsuyoshi Morita, Mayu Miyagi and Yoshizo Matsuka : Dentin-pulp complex tissue regeneration via three-dimensional cell sheet layering, Tissue Engineering. Part C, Methods, Vol.27, No.10, 559-570, 2021. (Summary) The dentin-pulp complex is a unique structure in teeth that contains both hard and soft tissues. Generally, deep caries and trauma cause damage to the dentin-pulp complex, and if left untreated, this damage will progress to irreversible pulpitis. The aim of this study was to fabricate a layered cell sheet composed of rat dental pulp (DP) cells and odontogenic differentiation of pulp (OD) cells and to investigate the ability to regenerate the dentin-pulp complex in a scaffold tooth. We fabricated two single cell sheets composed of DP cells (DP cell sheet) or OD cells (OD cell sheet) and a layered cell sheet made by layering both cells. The characteristics of the fabricated cell sheets were analyzed using light microscopy, scanning electron microscope (SEM), hematoxylin-eosin (HE) staining, and immunohistochemistry (IHC). Furthermore, the cell sheets were transplanted into the subrenal capsule of immunocompromised mice for 8 weeks. After this, the regenerative capacity to form dentin-like tissue was evaluated using micro-computed tomography (micro-CT), HE staining, and IHC. The findings of SEM and IHC confirmed that layered cell sheets fabricated by stacking OD cells and DP cells maintained their cytological characteristics. Micro-CT of layered cell sheet transplants revealed a mineralized capping of the access cavity in the crown area, similar to that of natural dentin. In contrast, the OD cell sheet group demonstrated the formation of irregular fragments of mineralized tissue in the pulp cavity, and the DP cell sheet did not develop any hard tissue. Moreover, bone volume/tissue volume (BV/TV) showed a significant increase in hard tissue formation in the layered cell sheet group compared with that in the single cell sheet group ( < 0.05). HE staining also showed a combination of soft and hard tissue formation in the layered cell sheet group. Furthermore, IHC confirmed that the dentin-like tissue generated from the layered cell sheet expressed characteristic markers of dentin but not bone equivalent to that of a natural tooth. In conclusion, this study demonstrates the feasibility of regenerating dentin-pulp complex using a bioengineered tissue designed to simulate the anatomical structure. Impact statement The dentin-pulp complex can be destroyed by deep caries and trauma, which may cause pulpitis and progress to irreversible pulpitis, apical periodontitis, and even tooth loss. Current treatments cannot maintain pulp health, and teeth can become brittle. We developed a three-dimensional (3D) layered cell sheet using dental pulp cells and odontogenic differentiation of pulp cells for dentin-pulp complex regeneration. Our layered cell sheet enables the regeneration of an organized 3D dentin-pulp-like structure comparable with that of natural teeth. This layered cell sheet technology may contribute to dentin-pulp complex regeneration and provide a novel method for complex tissue engineering. (Tokushima University Institutional Repository) ● Metadata: 117029 (Link to Publication Site) ● Publication site (DOI): 10.1089/ten.TEC.2021.0171 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34583551 ● Search Scopus @ Elsevier (PMID): 34583551 ● Search Scopus @ Elsevier (DOI): 10.1089/ten.TEC.2021.0171 (Tokushima University Institutional Repository: 117029, DOI: 10.1089/ten.TEC.2021.0171, PubMed: 34583551)
5.	Han Zhu, Zhang Weizhi, Ning Wanshan, Wang Chenwei, Deng Wankun, Li Zhidan, Shang Zehua, Shen Xiaofei, Liu Xiaohui, Otto Baba, Tsuyoshi Morita, Chen Lu, Xue Yu and Jia Da : Model-based analysis uncovers mutations altering autophagy selectivity in human cancer, Nature Communications, Vol.12, No.3258, 1-18, 2021. (Summary) Autophagy can selectively target protein aggregates, pathogens, and dysfunctional organelles for the lysosomal degradation. Aberrant regulation of autophagy promotes tumorigenesis, while it is far less clear whether and how tumor-specific alterations result in autophagic aberrance. To form a link between aberrant autophagy selectivity and human cancer, we establish a computational pipeline and prioritize 222 potential LIR (LC3-interacting region) motif-associated mutations (LAMs) in 148 proteins. We validate LAMs in multiple proteins including ATG4B, STBD1, EHMT2 and BRAF that impair their interactions with LC3 and autophagy activities. Using a combination of transcriptomic, metabolomic and additional experimental assays, we show that STBD1, a poorly-characterized protein, inhibits tumor growth via modulating glycogen autophagy, while a patient-derived W203C mutation on LIR abolishes its cancer inhibitory function. This work suggests that altered autophagy selectivity is a frequently-used mechanism by cancer cells to survive during various stresses, and provides a framework to discover additional autophagy-related pathways that influence carcinogenesis. (Keyword) Algorithms / Animals / Carcinogenesis / Cell Line, Tumor / Computer Simulation / DNA Mutational Analysis / Datasets as Topic / Gene Knockdown Techniques / Glycogen / Humans / Kaplan-Meier Estimate / Macroautophagy / Membrane Proteins / Mice / Microtubule-Associated Proteins / Models, Genetic / Muscle Proteins / Mutation / Neoplasms / Pentose Phosphate Pathway / Protein Interaction Domains and Motifs / Proteome / RNA-Seq / Tissue Array Analysis / Warburg Effect, Oncologic / Xenograft Model Antitumor Assays (Tokushima University Institutional Repository) ● Metadata: 116604 (Link to Publication Site) ● Publication site (DOI): 10.1038/s41467-021-23539-5 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34059679 ● Search Scopus @ Elsevier (PMID): 34059679 ● Search Scopus @ Elsevier (DOI): 10.1038/s41467-021-23539-5 (Tokushima University Institutional Repository: 116604, DOI: 10.1038/s41467-021-23539-5, PubMed: 34059679)
6.	Tsuyoshi Kano, Tsuyoshi Morita, Kaori Sumida, Hiromichi Yumoto and Otto Baba : Expression of fibroblast growth factor receptor1, -2c, and -3c transcripts in mouse molars after tooth eruption, Anatomical Science International, 2021. (Keyword) Dentin formation / FGF receptor / Odontoblasts / Secondary dentin / Tooth eruption (Tokushima University Institutional Repository) ● Metadata: 116058 (Link to Publication Site) ● Publication site (DOI): 10.1007/s12565-020-00594-4 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85099170531 (Tokushima University Institutional Repository: 116058, DOI: 10.1007/s12565-020-00594-4, Elsevier: Scopus)
7.	Resmi Raju, Masamitsu Ohshima, Miho Inoue, Tsuyoshi Morita, Yan Huijiao, Arief Waskitho, Otto Baba, Masahisa Inoue and Yoshizo Matsuka : Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet, Scientific Reports, Vol.10, No.1, 1656, 2020. (Summary) Periodontal tissue is a distinctive tissue structure composed three-dimensionally of cementum, periodontal ligament (PDL) and alveolar bone. Severe periodontal diseases cause fundamental problems for oral function and general health, and conventional dental treatments are insufficient for healing to healthy periodontal tissue. Cell sheet technology has been used in many tissue regenerations, including periodontal tissue, to transplant appropriate stem/progenitor cells for tissue regeneration of a target site as a uniform tissue. However, it is still difficult to construct a three-dimensional structure of complex tissue composed of multiple types of cells, and the transplantation of a single cell sheet cannot sufficiently regenerate a large-scale tissue injury. Here, we fabricated a three-dimensional complex cell sheet composed of a bone-ligament structure by layering PDL cells and osteoblast-like cells on a temperature responsive culture dish. Following ectopic and orthotopic transplantation, only the complex cell sheet group was demonstrated to anatomically regenerate the bone-ligament structure along with the functional connection of PDL-like fibers to the tooth root and alveolar bone. This study represents successful three-dimensional tissue regeneration of a large-scale tissue injury using a bioengineered tissue designed to simulate the anatomical structure. (Tokushima University Institutional Repository) ● Metadata: 114213 (Link to Publication Site) ● Publication site (DOI): 10.1038/s41598-020-58222-0 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32015383 ● Search Scopus @ Elsevier (PMID): 32015383 ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-020-58222-0 (Tokushima University Institutional Repository: 114213, DOI: 10.1038/s41598-020-58222-0, PubMed: 32015383)
8.	Takuma Iwasa, S Afroz, Miho Inoue, Rieko Arakaki, Masamitsu Ohshima, R Raju, Arief Waskitho, Inoue Masahisa, Otto Baba and Yoshizo Matsuka : IL-10 and CXCL2 in trigeminal ganglia in neuropathic pain, Neuroscience Letters, Vol.703, 132-138, 2019. (Tokushima University Institutional Repository) ● Metadata: 113686 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.neulet.2019.03.031 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.neulet.2019.03.031 (Tokushima University Institutional Repository: 113686, DOI: 10.1016/j.neulet.2019.03.031)
9.	Elizabeth Haythorne, Maria Rohm, Martijn de Bunt van, F Melissa Brereton, I Andrei Tarasov, S Thomas Blacker, Gregor Sachse, Mariana Dos Santos Silva, Raul Exposito Terron, Simon Davis, Otto Baba, Roman Fischer, R Michael Duchen, Patrik Rorsman, I James MacRae and M Frances Ashcroft : Diabetes causes marked inhibition of mitochondrial metabolism in pancreatic β-cells., Nature Communications, Vol.10, No.1, 2019. (Summary) Diabetes is a global health problem caused primarily by the inability of pancreatic β-cells to secrete adequate levels of insulin. The molecular mechanisms underlying the progressive failure of β-cells to respond to glucose in type-2 diabetes remain unresolved. Using a combination of transcriptomics and proteomics, we find significant dysregulation of major metabolic pathways in islets of diabetic βV59M mice, a non-obese, eulipidaemic diabetes model. Multiple genes/proteins involved in glycolysis/gluconeogenesis are upregulated, whereas those involved in oxidative phosphorylation are downregulated. In isolated islets, glucose-induced increases in NADH and ATP are impaired and both oxidative and glycolytic glucose metabolism are reduced. INS-1 β-cells cultured chronically at high glucose show similar changes in protein expression and reduced glucose-stimulated oxygen consumption: targeted metabolomics reveals impaired metabolism. These data indicate hyperglycaemia induces metabolic changes in β-cells that markedly reduce mitochondrial metabolism and ATP synthesis. We propose this underlies the progressive failure of β-cells in diabetes. (Keyword) Adenosine Triphosphate / Animals / Diabetes Mellitus, Experimental / Diabetes Mellitus, Type 2 / Gene Expression Profiling / Gluconeogenesis / Glucose / Glycolysis / Insulin Secretion / Insulin-Secreting Cells / Metabolomics / Mice / Mice, Transgenic / Mitochondria / NAD / Oxidative Phosphorylation / Oxygen Consumption / Potassium Channels, Inwardly Rectifying / Proteomics (Tokushima University Institutional Repository) ● Metadata: 115227 (Link to Publication Site) ● Publication site (DOI): 10.1038/s41467-019-10189-x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31171772 ● Search Scopus @ Elsevier (PMID): 31171772 ● Search Scopus @ Elsevier (DOI): 10.1038/s41467-019-10189-x (Tokushima University Institutional Repository: 115227, DOI: 10.1038/s41467-019-10189-x, PubMed: 31171772)
10.	S Afroz, Rieko Arakaki, Takuma Iwasa, Masamitsu Ohshima, Maki Hosoki, Miho Inoue, Otto Baba, Yoshihiro Okayama and Yoshizo Matsuka : CGRP induces differential regulation of cytokines from satellite glial cells in trigeminal ganglia and orofacial nociception, International Journal of Molecular Sciences, Vol.20, No.3, 711, 2019. (Summary) Neuron-glia interactions contribute to pain initiation and sustainment. Intra-ganglionic (IG) secretion of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) modulates pain transmission through neuron-glia signaling, contributing to various orofacial pain conditions. The present study aimed to investigate the role of satellite glial cells (SGC) in TG in causing cytokine-related orofacial nociception in response to IG administration of CGRP. For that purpose, CGRP alone (10 μL of 10 M), Minocycline (5 μL containing 10 μg) followed by CGRP with one hour gap (Min + CGRP) were administered directly inside the TG in independent experiments. Rats were evaluated for thermal hyperalgesia at 6 and 24 h post-injection using an operant orofacial pain assessment device (OPAD) at three temperatures (37, 45 and 10 °C). Quantitative real-time PCR was performed to evaluate the mRNA expression of IL-1β, IL-6, TNF-α, IL-1 receptor antagonist (IL-1RA), sodium channel 1.7 (NaV 1.7, for assessment of neuronal activation) and glial fibrillary acidic protein (GFAP, a marker of glial activation). The cytokines released in culture media from purified glial cells were evaluated using antibody cytokine array. IG CGRP caused heat hyperalgesia between 6-24 h (paired- test, < 0.05). Between 1 to 6 h the mRNA and protein expressions of GFAP was increased in parallel with an increase in the mRNA expression of pro-inflammatory cytokines IL-1β and anti-inflammatory cytokine IL-1RA and NaV1.7 (one-way ANOVA followed by Dunnett's post hoc test, < 0.05). To investigate whether glial inhibition is useful to prevent nociception symptoms, Minocycline (glial inhibitor) was administered IG 1 h before CGRP injection. Minocycline reversed CGRP-induced thermal nociception, glial activity, and down-regulated IL-1β and IL-6 cytokines significantly at 6 h (-test, < 0.05). Purified glial cells in culture showed an increase in release of 20 cytokines after stimulation with CGRP. Our findings demonstrate that SGCs in the sensory ganglia contribute to the occurrence of pain via cytokine expression and that glial inhibition can effectively control the development of nociception. (Tokushima University Institutional Repository) ● Metadata: 113687 (Link to Publication Site) ● Publication site (DOI): 10.3390/ijms20030711 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30736422 ● Summary page in Scopus @ Elsevier: 2-s2.0-85061270594 (Tokushima University Institutional Repository: 113687, DOI: 10.3390/ijms20030711, PubMed: 30736422, Elsevier: Scopus)
11.	Furukawa Fumiya, Irachi Shotaro, Koyama Mugen, Otto Baba, Akimoto Hajime, Okumura Sei-ichi, Kagawa Hirohiko and Uchida Katsuhisa : Changes in glycogen concentration and gene expression levels of glycogen-metabolizing enzymes in muscle and liver of developing masu salmon., Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology, Vol.225, 72-84, 2018. (Summary) Glycogen, as an intracellular deposit of polysaccharide, takes important roles in energy balance of many animals. In fish, however, the role of glycogen during development is poorly understood. In the present study, we assessed changes in glycogen concentration and gene expression patterns of glycogen-metabolizing enzymes in developing masu salmon (Oncorhynchus masou masou), a salmonid species inhabiting west side of North Pacific Ocean. As we measured glycogen levels in the bodies and yolk sacs containing the liver separately, the glycogen concentration increased in both parts as the fish developed, whereas it transiently decreased in the yolk sac after hatching, implying glycogen synthesis and breakdown in these tissues. Immunofluorescence staining using anti-glycogen monoclonal antibody revealed localization of glycogen in the liver, muscle and yolk syncytial layer of the pre-hatching embryos and hatched larvae. In order to estimate glycogen metabolism in the fish, the genes encoding homologs of glycogen synthase (gys1 and gys2) and glycogen phosphorylase (pygma, pygmb and pygl) were cloned, and their expression patterns were assessed by quantitative PCR and in situ hybridization. In the fish, gys1 and gys2 were robustly expressed in the muscle and liver, respectively. Also, expression of pyg isoforms was found in muscle, liver and yolk syncytial layer during hatching. With changes in glycogen concentration and expression patterns of relevant genes, our results suggest, for the first time, possible involvement of glycogen in energy balance of fish embryos, especially during hatching. (Keyword) Glycogen synthase / Glycogen phosphorylase / energy metabolism / Fish development / Hatching (Link to Publication Site) ● Publication site (DOI): 10.1016/j.cbpa.2018.07.003 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30017911 ● Search Scopus @ Elsevier (PMID): 30017911 ● Search Scopus @ Elsevier (DOI): 10.1016/j.cbpa.2018.07.003 (DOI: 10.1016/j.cbpa.2018.07.003, PubMed: 30017911)
12.	Kaori Sumida, Yoshinori Ando, Shinichiro Seki, Kikuji Yamashita, Akira Fujimura, Otto Baba and Seiichiro Kitamura : Anatomical status of the human palatopharyngeal sphincter and its functional implications., Surgical and Radiologic Anatomy : SRA, Vol.39, No.11, 1191-1201, 2017. (Summary) The PPS is a muscle distinct from the SCP. Its contraction produces Passavant's ridge and conceivably enhances the efficiency of velopharyngeal closure by pressing the salpingopharyngeal fold and the musculus uvulae ridge against the velum. (Link to Publication Site) ● Publication site (DOI): 10.1007/s00276-017-1855-6 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28447150 ● Search Scopus @ Elsevier (PMID): 28447150 ● Search Scopus @ Elsevier (DOI): 10.1007/s00276-017-1855-6 (DOI: 10.1007/s00276-017-1855-6, PubMed: 28447150)
13.	Shinichiro Seki, Kaori Sumida, Kikuji Yamashita, Otto Baba and Seiichiro Kitamura : Gross anatomical classification of the courses of the human lingual artery., Surgical and Radiologic Anatomy : SRA, Vol.39, No.2, 195-203, 2017. (Summary) The present study provides detailed information on the courses of lingual artery which will be of clinical importance especially in the super-selective arterial angiography. (Keyword) Gross anatomical classification / human lingual artery (Link to Publication Site) ● Publication site (DOI): 10.1007/s00276-016-1696-8 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27189234 ● Summary page in Scopus @ Elsevier: 2-s2.0-84969247767 (DOI: 10.1007/s00276-016-1696-8, PubMed: 27189234, Elsevier: Scopus)
14.	Kikuji Yamashita, Dalkhsuren Shine-Od, Aldartsogt Dolgorsuren, Kaori Sumida, Tsuyoshi Morita and Otto Baba : Effects of Dietary Cure Containing AFIR (Antioxidant Far-Infrared Energy Radiating) Ceramics on Living Body of Hatchery Sea Bream (Pagrus Major), International Journal of Innovative Studies in Sciences and Engineering Technology, Vol.2, No.10, 7-16, 2016.
15.	Tsuyoshi Morita, Kaoru Fujikawa, Otto Baba and Shibata Shunichi : An in situ hybridization study of Hyaluronan synthase (Has) mRNA in developing mouse molar and incisor tooth germs, Gene Expression Patterns, Vol.21, No.1, 28-40, 2016. (Summary) Hyaluronan (HA) is a major constituent molecule in most extracellular matrices and is synthesized by Hyaluronan synthase (Has). In the present study, we examined expression patterns of Has1, -2, -3 mRNA in developing mouse molar and incisor tooth germs from embryonic day (E) 11.5 to postnatal day (P) 7, focusing on Hertwig's epithelial root sheath (HERS) and the apical bud in particular. Has1 mRNA expression was not detected in all tooth germs examined. Has2 mRNA was expressed in the surrounding mesenchyme from E12.0 to 18.0 in both molar and incisor tooth germs, but disappeared after birth. Meanwhile, Has3 mRNA was exclusively expressed within the enamel organ, especially in the inner enamel epithelium (IEE), stellate reticulum (SR), and stratum intermedium (SI) until the early bell stage at E16.0. Has3 mRNA disappeared as IEE differentiated into differentiating ameloblasts (dABs), but remained in SI until the root developmental stage of the molar tooth germ at P7. Has3 mRNA was also expressed in HERS until P7. In incisors, Has3 mRNA was expressed in the apical bud, especially in the transit-amplifying (TA) cell region from E16.0 to P7, and in the papillary layer (PL) adjacent to the mature enamel. These gene expression patterns suggested that Has3 is the main control factor for prenatal and postnatal HA synthesis of the tooth germ, and may in part regulate crown and root formation of the tooth germ, maintenance of stem cell niches in the apical bud as well as mineral transport in PL. (Keyword) Animals / Embryonic Development / Enamel Organ / Gene Expression Regulation, Developmental / Glucuronosyltransferase / Hyaluronic Acid / In Situ Hybridization / Incisor / Mesoderm / Mice / Molar / Odontogenesis / RNA, Messenger / Tooth Germ (Link to Publication Site) ● Publication site (DOI): 10.1016/j.gep.2016.06.002 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27289075 ● Search Scopus @ Elsevier (PMID): 27289075 ● Search Scopus @ Elsevier (DOI): 10.1016/j.gep.2016.06.002 (DOI: 10.1016/j.gep.2016.06.002, PubMed: 27289075)
16.	Otto Baba : Production of monoclonal antibody that recognizes glycogen and its application for immunohistochemistry, The Journal of the Stomatological Society, Japan, Vol.60, No.2, 264-287, 1993. (Keyword) antibody / glycogen / immunohistochemistry

Review, Commentary:

1.	Kaori Sumida, Tsuyoshi Morita and Otto Baba : Considering the Morphological and Functional Implications of the Human Palatopharyngeus, Journal of Oral Health and Biosciences, Vol.34, No.2, 19-25, Mar. 2022. (Keyword) palatopaharyngeus / hyoid bone / human (Tokushima University Institutional Repository) ● Metadata: 116766 (Link to Publication Site) ● Publication site (DOI): 10.20738/johb.34.2_19 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390573242509540096 ● Search Scopus @ Elsevier (DOI): 10.20738/johb.34.2_19 (Tokushima University Institutional Repository: 116766, DOI: 10.20738/johb.34.2_19, CiNii: 1390573242509540096)
2.	Otto Baba : 知れば安心，もっと楽しい! 解剖学的口腔の観かた∼下顎骨を知る第6回下顎骨の成長・歯の喪失による形態変化, The Journal of Dental Hygiene, Vol.40, No.4, 346-350, Mar. 2020.
3.	Otto Baba : 知れば安心，もっと楽しい! 解剖学的口腔の観かた∼下顎骨を知る第5回下顎骨に付着する筋肉と咀嚼・嚥下運動, The Journal of Dental Hygiene, Vol.40, No.3, 236-239, Feb. 2020.
4.	Otto Baba : 知れば安心，もっと楽しい! 解剖学的口腔の観かた∼下顎骨を知る第4回口腔壁を構成する下顎に付着する筋, The Journal of Dental Hygiene, Vol.40, No.2, 122-125, Jan. 2020.
5.	Otto Baba : 知れば安心，もっと楽しい! 解剖学的口腔の観かた∼下顎骨を知る第3回下顎骨の基本構造③(下顎孔と下顎管と関節突起について), The Journal of Dental Hygiene, Vol.40, No.1, 12-15, Dec. 2019.
6.	Otto Baba : 知れば安心，もっと楽しい! 解剖学的口腔の観かた∼下顎骨を知る第2回下顎骨の基本構造②(下顎枝について), The Journal of Dental Hygiene, Vol.39, No.12, 1240-1242, Nov. 2019.
7.	Otto Baba : 知れば安心，もっと楽しい! 解剖学的口腔の観かた∼下顎骨を知る第1回下顎骨の基本構造①(概要と下顎体について), The Journal of Dental Hygiene, Vol.39, No.11, 1127-1129, Oct. 2019.
8.	Otto Baba : The New Insight into Radicular Dentin Formation, Journal of Oral Health and Biosciences, Vol.29, No.1, 1-6, Jun. 2016. (Summary) Dentin is mineralized connective tissue, which consists of two parts, coronal dentincovered by enamel and radicular dentin by cementum. Although both parts are formed by odontoblasts, biophysical and biochemical properties are indeed different, which evoke the specific system for radicular dentin formation. One of the critical factor of radicular dentin formation is Hertwig's epithelial root sheath (HERS), which leads proliferation and differentiation of odontoblasts under the control of TGF-/BMP signaling, and the ablation or over-expression of constituent molecules showed the specific malformation of radicular dentin. Wnt/-catenin signaling is the other candidate of dentin formation which may regulate differently on crown and root formation. Fibroblast growth factor (FGF) 18 is possible downstream molecule of this signaling. Interestingly, the expression of FGF18 transcripts was detected during root formation, but not crown formation in rat mandibular molar.While possible receptors, FGFR2 and FGFR3, were continuously observed in both crown and root formation, those suggested that coronal and radicular dentin might be formed under the continuous and/or reciprocal control of different FGFs. The clarification of these systems may open new insight into the tissue regeneration and/or engineering of tooth and periodontium. (Tokushima University Institutional Repository) ● Metadata: 109874 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050001337465025408 (Tokushima University Institutional Repository: 109874, CiNii: 1050001337465025408)

Proceeding of International Conference:

1.	S Afroz, Rieko Arakaki, Takuma Iwasa, Masamitsu Ohshima, Maki Hosoki, Miho Inoue, Otto Baba, Yoshihiro Okayama and Yoshizo Matsuka : CGRP induced glial-cytokine expression, NFkB signaling and orofacial nociception, International Association for Dental Research, Washington, D.C., Mar. 2020.
2.	Otto Baba : Regulation of Odontoblasts in Various Phases of Dentin Formation, DENTISPHERE 4 (International Scientific Meeting 5th Asean Plus & Tokushima Joint International Conference), Surabaya, Indonesia, Nov. 2019.
3.	R Raju, Masamitsu Ohshima, Tsuyoshi Morita, Huijiao Yan, Miho Inoue, Otto Baba, Masahisa Inoue and Yoshizo Matsuka : A double-layered complex cell sheet can regenerate the periodontal tissue in a mice model, Dentisphere, Surabayai, Nov. 2019.
4.	S Afroz, Rieko Arakaki, Takuma Iwasa, Masamitsu Ohshima, Otto Baba, Y Okayama and Yoshizo Matsuka : Orofacial pain due to glial expressed cytokines in trigeminal ganglion, Dentisphere, Surabayai, Nov. 2019.
5.	Arief Waskitho, Yumiko Yamamoto, Tsuyoshi Morita, Huijiao Yan, R Raju, Masamitsu Ohshima, Junhel Dalanon, Otto Baba and Yoshizo Matsuka : Bilateral effects of unilateral administration of botulinum toxin A in chemotherapy induced neuropathy, Dentisphere, Surabayai, Nov. 2019.
6.	Otto Baba : Symposium: Perspectives on the Impact of William Butler's Mentoring, 97th General session & exhibition of the IADR, Vancouver, Jun. 2019.
7.	S Afroz, Rieko Arakaki, Takuma Iwasa, Masamitsu Ohshima, Maki Hosoki, Miho Inoue, Otto Baba, Yoshihiro Okayama and Yoshizo Matsuka : Orofacial nociception due to glial expressed cytokines in trigeminal ganglia, International Association for Dental Research, Vancouver, Jun. 2019.
8.	Kazumitsu Sekine, Yoshihito Naitou, Tetsuo Ichikawa, Otto Baba and Kenichi Hamada : Development of structural and chemical enforcement of neointimal growth as the blood contacting surface for the vascular prosthesis, 45th European Society for Artificial Organs 2018 Congress, Madrid, Sep. 2018.
9.	Y Kumei, J Zeredo, R Watahiki, K Kagiyama, K Fukasawa and Otto Baba : Common marmosets in Martian, Lunar, and zero gravity conditions: posture, behavior and motion sickness., The Joint Conference of the 7th International Symposium on Physical Sciences in Space & 25th European Low Gravity Research Association Biennial Symposium and General Assembly, Oct. 2017.
10.	R Watahiki, J Zeredo, K Kagiyama, H Hashimoto, N Ishioka, Y Inatomi, Otto Baba, S Aou, K Natsume and Y Kumei : Postural and behavioral of primates (Common Marmoset) to Martian, Lunar, and Zero gravity conditions., American Society for Gravitational and Space Research Annual Meeting, Oct. 2016.

Proceeding of Domestic Conference:

1.	Mikiya Satoh, Kaho Yumoto, Tsuyoshi Morita, Kaori Sumida and Otto Baba : Involvement of sox genes during the formation of medaka pharyngeal tooth and pedicle, 第64回歯科基礎医学会学術大会, Sep. 2022.
2.	Yui Ohno, Ryutaroh Tohgoh, Ayako Yamane, Tsuyoshi Morita, Kaori Sumida and Otto Baba : マウス象牙質の形成過程におけるFgf18の発現, 四国歯学会第60回例会, Jun. 2022.
3.	Waskitho Arief, Yumiko Yamamoto, Raman Swarna Lakshmi, Fumiya Kano, Huijiao Yan, Kazuo Okura, Daisuke Ikutame, Masamitsu Ohshima, Otto Baba, Akihito Yamamoto and Yoshizo Matsuka : Bilateral botulinum toxin type A effect on orofacial neuropathic pain of animal model, 徳島大学脳科学クラスターミニリトリート, Feb. 2022.
4.	Masahito Kawano, Mikiya Satoh, Kaho Yumoto, Kaori Sumida, Tsuyoshi Morita, 神尾強司 and Otto Baba : 口蓋垂裂の一例の肉眼解剖学的および組織学的観察, 四国歯学会第57回例会, Mar. 2021.
5.	Kazumitsu Sekine, Otto Baba and Kenichi Hamada : 表面修飾を施した多孔質チタンインプラントの組織封鎖性評価, 第58回日本人工臓器学会大会, Nov. 2020.
6.	Kazumitsu Sekine, Otto Baba and Kenichi Hamada : 骨代替インプラントを目的とした封鎖性の高いチタンスキャフォールドの開発, 第59回日本生体医工学会大会, May 2020.
7.	Kaori Sumida, Seiichiro Kitamura, Tsuyoshi Morita, 神尾強司, 城翠, 古田琴, 濱義之, 田畑純 and Otto Baba : コモンリスザルの軟口蓋・咽頭の筋構築に関する肉眼解剖学的研究, 第125回日本解剖学会総会・全国学術集会講演プログラム・抄録集山口 2020 誌上開催, 162, Mar. 2020.
8.	Yui Miyagawa, Masaki Gotoh, Yukari Hatada, Tsuyoshi Morita, Tsuyoshi Kano, Kaori Sumida and Otto Baba : マダイ稚魚の歯の形成過程に関する組織学的研究, The 125th Annual Meeting of The Japanese Association of Anatomists, Mar. 2020.
9.	Tsuyoshi Morita, Tsuyoshi Kano, Kaori Sumida and Otto Baba : 歯・歯周組織の形成機構の解析および再生への応用の検討, 四国歯学会第56回例会, Feb. 2020.
10.	Shin Matsumoto, Soichiroh Kinjoh, Tsuyoshi Morita, Kaori Sumida, Tsuyoshi Kano and Otto Baba : メダカ咽頭歯における歯および歯周組織の形成遺伝子の発現, 四国歯学会第56回例会, 34, Feb. 2020.
11.	Takuma Iwasa, Afroz Shaista, Miho Inoue, Rieko Arakaki, Masamitsu Ohshima, Raju Resmi, Arief Waskitho, 井上正久, Otto Baba and Yoshizo Matsuka : 痛みに対するサイトカイン療法の開発に向けて, 徳島県歯科医学大会, Feb. 2020.
12.	Tsuyoshi Kano, Tsuyoshi Morita, Kaori Sumida, Hiromichi Yumoto and Otto Baba : Gene expression of dentin formation related factors in dental pulp of mouse molar, after tooth eruption, 第61回歯科基礎医学会学術大会, Oct. 2019. (Keyword) pulp / 象牙質 / in situ hybridization
13.	Waskitho Arief, Yamamoto Yumiko, Tsuyoshi Morita, Yan Huijiao, Raju Resmi, Masamitsu Ohshima, Dalanon Junhel, Otto Baba and Yoshizo Matsuka : Orofacial neuropathic pain rat models induced by chemotherapy drugs, Tokushima University Bioscience Retreat, Sep. 2019.
14.	Raju Resmi, Masamitsu Ohshima, Miho Inoue, Tsuyoshi Morita, Yan Huijiao, Otto Baba, Inoue Masahisa and Yoshizo Matsuka : Development of a multilayered complex cell sheet to regenerate periodontal tissue, Tokushima University Bioscience Retreat, Sep. 2019.
15.	Raju Resmi, Masamitsu Ohshima, Miho Inoue, Tsuyoshi Morita, Yan Huijiao, Otto Baba, 井上正久 and Yoshizo Matsuka : 骨芽細胞と歯根膜細胞との立体形成複合細胞シートによる歯周組織再生の確立, 硬組織再生生物学会, Aug. 2019.
16.	Kazumitsu Sekine, Otto Baba and Kenichi Hamada : 細胞賦活化処理による多孔質性状チタン足場材の生体内癒合性の向上を狙う, 第58回日本生体医工学会大会, Jun. 2019.
17.	Kaori Sumida, Seiichiro Kitamura, Tsuyoshi Morita, 神尾強司, 田畑純 and Otto Baba : ヒト，ウマ，イヌの茎突咽頭筋の走行・停止の比較からヒト茎突咽頭筋の機能を考える, 第124回日本解剖学会総会全国学術集会, Mar. 2019.
18.	Shin Matsumoto, Soichiroh Kinjoh, Sohshi Fujita, Tsuyoshi Morita, Tsuyoshi Kano, Kaori Sumida, Asuna Sugimoto, Tsutomu Iwamoto and Otto Baba : メダカ咽頭歯における分泌性カルシウム結合リンタンパク質(SCPP)遺伝子の発現, 第124回日本解剖学会総会・全国学術集会, Mar. 2019. (Keyword) SCPP / メダカ / 咽頭歯 / in situ hybridization
19.	Kazumitsu Sekine, Otto Baba and Kenichi Hamada : 細胞賦活性を施した多孔質チタン足場材に関する生体内癒合性評価, 第56回日本人工臓器学会大会, Nov. 2018.
20.	金城聡一郎, Shin Matsumoto, 藤田創詩, Kaori Sumida, Tsuyoshi Morita, 神尾強司 and Otto Baba : 馬蹄腎の一例についての肉眼解剖学的および組織学的観察, 四国歯学会第53回例会・第37回総会, Jul. 2018.
21.	Kazumitsu Sekine, Otto Baba and Kenichi Hamada : Evaluation of Titanium surface modification for hard tissue prostheses, 第57回日本生体医工学会大会, Jun. 2018.
22.	Kaori Sumida, Seiichiro Kitamura, 神尾強司, Tsuyoshi Morita, Kikuji Yamashita and Otto Baba : イヌの軟口蓋の筋構築に関する肉眼解剖学的研究, 日本解剖学会総会・第123回全国学術集会,, Mar. 2018.
23.	Kaori Sumida, Seiichiro Kitamura, Tsuyoshi Morita, Kikuji Yamashita and Otto Baba : Gross anatomical study of the human stylopharyngeus: origin, course,and insertion., The 122nd annual meeting of the Japanese association of anatomists, Mar. 2017.

Et cetera, Workshop:

1.	Raju Resmi, Masamitsu Ohshima, Tsuyoshi Morita, Yan Huijiao, Miho Inoue, Otto Baba, Inoue Masahisa and Yoshizo Matsuka : Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet, 発生・再生・遺伝クラスターミニリトリート, Feb. 2020.
2.	Waskitho Arief, Yumiko Yamamoto, Tsuyoshi Morita, Yan Huijiao, Raju Resmi, Masamitsu Ohshima, Dalanon Junhel, Laksmi Swarna, Chavan P., Otto Baba and Yoshizo Matsuka : Botulinum toxin A administration in chemotherapy-induced neuropathic pain rat model, 徳島大学脳科学クラスターミニリトリート, Feb. 2020.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

象牙芽細胞による基質形成のSwitching (Project/Area Number: 22K09903 )
Common marmoset as a primate model for gerontology in dentistry (Project/Area Number: 16K15852 )
The common marmoset, a new-world monkey as a human-alternative model for studies on pediatric dentistry (Project/Area Number: 26463107 )
New paradigm of enamel maturation: Mechanisms of non-endocytotic resorption and degradation of enamel matrix proteins (Project/Area Number: 24390408 )
The study on the cells forming periodontium by observing the expression of dentin proteins as the markers of differentiation (Project/Area Number: 18591996 )
Establishment of organ culture system for tooth regeneration using pharyngeal teeth of medaka and its use for the exploration of dental stem cells (Project/Area Number: 18390485 )
The induction of periodontal tissues by dentin-derived matrix (Project/Area Number: 14370577 )
Structural analysis of the role of enamel matrix proteins in regeneration of biological hard tissues (Project/Area Number: 12557151 )
骨、軟骨組織に加齢および外力が与える影響とその機構に関する解析 (Project/Area Number: 08771560 )
Biochemical and histological analysis of proteoglycans systhsized by cartilaginous cell line, ATDC5. (Project/Area Number: 07671963 )
Immunohistochemical and Enzyme-histochemical studies on the cellular networks of antigen-presenting cells in the dental and periodontal tissues. (Project/Area Number: 07457426 )
下顎頭軟骨に対するモノクローナル抗体の作製と免疫組織化学的観察 (Project/Area Number: 06771574 )
石灰化に伴う各種プロテオグリカンの構造変化に関する生化学的、組織化学的解析 (Project/Area Number: 06671806 )
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Professor : Baba, Otto

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)