Tokushima University / Educator and Researcher Directory --- Takanari, Hiroki

Personal Web Page

https://researchmap.jp/hiroki_takanari (researchmap)

https://orcid.org/0000-0001-9348-9877 (ORCID)

Field of Study

○	Life sciences [Biomedical engineering]
○	Life sciences [Clinical pharmacy]
○	Life sciences [Physiology]
○	Life sciences [Cardiology]
○	Life sciences [Clinical pharmacy]
○	Life sciences [Physiology]

Subject of Study

Book / Paper

Academic Paper (Judged Full Paper):

1.	Shin-ichiro Yanagiya, Takeshi Honda, Hiroki Takanari, Kimiko Sogabe, Shingen Nakamura, Yoshimi Bando, Masahiro Abe and Hirokazu Miki : Raman Microspectroscopy for Label-Free Diagnosis of Amyloid Light-chain Amyloidosis in Various Organs, Journal of Raman Spectroscopy, 2024. (Keyword) Raman microspectroscopy / Systemic amyloidosis / protein / light microscopy (Tokushima University Institutional Repository) ● Metadata: 119087 (Link to Publication Site) ● Publication site (DOI): 10.1002/jrs.6665 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1002/jrs.6665 (Tokushima University Institutional Repository: 119087, DOI: 10.1002/jrs.6665)
2.	Saya Matsuzaki, Eiji Hase, Hiroki Takanari, Yuri Hayashi, Yusaku Hayashi, Haruto Oshikata, Takeo Minamikawa, Satoko Kimura, Mayuko Shimizu, Takeshi Yasui, Masafumi Harada and Koichi Tsuneyama : Quantification of collagen fiber properties in alcoholic liver fibrosis using polarization-resolved second harmonic generation microscopy., Scientific Reports, Vol.13, No.1, 22100, 2023. (Summary) Liver fibrosis is assessed mainly by conventional staining or second harmonic generation (SHG) microscopy, which can only provide collagen content in fibrotic area. We propose to use polarization-resolved SHG (PR-SHG) microscopy to quantify liver fibrosis in terms of collagen fiber orientation and crystallization. Liver samples obtained from autopsy cases with fibrosis stage of F0-F4 were evaluated with an SHG microscope, and 12 consecutive PR-SHG images were acquired while changing the polarization azimuth angle of the irradiated laser from 0° to 165° in 15° increments using polarizer. The fiber orientation angle (φ) and degree (ρ) of collagen were estimated from the images. The SHG-positive area increased as the fibrosis stage progressed, which was well consistent with Sirius Red staining. The value of φ was random regardless of fibrosis stage. The mean value of ρ (ρ-mean), which represents collagen fiber crystallinity, varied more as fibrosis progressed to stage F3, and converged to a significantly higher value in F4 than in other stages. Spatial dispersion of ρ (ρ-entropy) also showed increased variation in the stage F3 and decreased variation in the stage F4. It was shown that PR-SHG could provide new information on the properties of collagen fibers in human liver fibrosis. (Keyword) Humans / Second Harmonic Generation Microscopy / Collagen / Liver Cirrhosis / Refraction, Ocular / Coloring Agents (Link to Publication Site) ● Publication site (DOI): 10.1038/s41598-023-48887-8 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 38092851 ● Search Scopus @ Elsevier (PMID): 38092851 ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-023-48887-8 (DOI: 10.1038/s41598-023-48887-8, PubMed: 38092851)
3.	Masatoshi Yamazaki, Naoki Tomii, Koichi Tsuneyama, Hiroki Takanari, Ryoko Niwa, Haruo Honjo, Itsuo Kodama, Tatsuhiko Arafune, Naomasa Makita, Ichiro Sakuma, Dobromir Dobrev, Stanley Nattel and Yukiomi Tsuji : Rotors anchored by refractory islands drive torsades de pointes in an experimental model of electrical storm., Heart Rhythm, Vol.19, No.2, 318-329, 2021. (Summary) current merits further investigation as a contributor to the substrate for ES. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.hrthm.2021.10.012 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34678525 ● Search Scopus @ Elsevier (PMID): 34678525 ● Search Scopus @ Elsevier (DOI): 10.1016/j.hrthm.2021.10.012 (DOI: 10.1016/j.hrthm.2021.10.012, PubMed: 34678525)
4.	Takeo Minamikawa, Mayuko Shimizu, Hiroki Takanari, Yuki Morimoto, Ryosuke Shiomi, Tanioka Hiroki, Eiji Hase, Takeshi Yasui and Koichi Tsuneyama : Molecular imaging analysis of microvesicular and macrovesicular lipid droplets in non-alcoholic fatty liver disease by Raman microscopy., Scientific Reports, Vol.10, No.1, 18548, 2020. (Summary) Predominant evidence of non-alcoholic fatty liver disease (NAFLD) is the accumulation of excess lipids in the liver. A small group with NAFLD may have a more serious condition named non-alcoholic steatohepatitis (NASH). However, there is a lack of investigation of the accumulated lipids with spatial and molecular information. Raman microscopy has the potential to characterise molecular species and structures of lipids based on molecular vibration and can achieve high spatial resolution at the organelle level. In this study, we aim to demonstrate the feasibility of Raman microscopy for the investigation of NAFLD based on the molecular features of accumulated lipids. By applying the Raman microscopy to the liver of the NASH model mice, we succeeded in visualising the distribution of lipid droplets (LDs) in hepatocytes. The detailed analysis of Raman spectra revealed the difference of molecular structural features of the LDs, such as the degree of saturation of lipids in the LDs. We also found that the inhomogeneous distribution of cholesterol in the LDs depending on the histology of lipid accumulation. We visualised and characterised the lipids of NASH model mice by Raman microscopy at organelle level. Our findings demonstrated that the Raman imaging analysis was feasible to characterise the NAFLD in terms of the molecular species and structures of lipids. (Tokushima University Institutional Repository) ● Metadata: 115619 (Link to Publication Site) ● Publication site (DOI): 10.1038/s41598-020-75604-6 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33122711 ● Search Scopus @ Elsevier (PMID): 33122711 ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-020-75604-6 (Tokushima University Institutional Repository: 115619, DOI: 10.1038/s41598-020-75604-6, PubMed: 33122711)
5.	Eiji Hase, Hiroki Takanari, Keita Hoshi, Masaki Okamoto, Atsushi Tabata, Hideaki Nagamune, Takeo Minamikawa, Takeshi Yasui, Yasushi Yoshida, Keiji Minagawa, Yasuhiko Kawamura, Yasushi Imada and Fumitoshi Yagishita : Two- and three-photon excitable quaternized imidazo[1,2-a]pyridines for mitochondrial imaging and potent cancer therapy agent, Organic & Biomolecular Chemistry, Vol.18, No.38, 7571-7576, 2020. (Link to Publication Site) ● Publication site (DOI): 10.1039/D0OB01585G (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32940324 ● Search Scopus @ Elsevier (PMID): 32940324 ● Search Scopus @ Elsevier (DOI): 10.1039/D0OB01585G (DOI: 10.1039/D0OB01585G, PubMed: 32940324)
6.	Satoru Hashimoto, Hiroki Takanari, Emmanuel Compe and Jean-Marc Egly : Dysregulation of LXR responsive genes contribute to ichthyosis in Trichonthiodystrophy, Journal of Dermatological Science, Vol.97, No.3, 201-207, 2020. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jdermsci.2020.01.012 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.jdermsci.2020.01.012 (DOI: 10.1016/j.jdermsci.2020.01.012)
7.	Fumitoshi Yagishita, Jun-ichi Tanigawa, Chiho Nii, Atsushi Tabata, Hideaki Nagamune, Hiroki Takanari, Yasushi Imada and Yasuhiko Kawamura : Fluorescent Imidazo[1,5-a]pyridinium Salt for a Potential Cancer Therapy Agent, ACS Medicinal Chemistry Letters, Vol.10, No.8, 1110-1114, 2019. (Link to Publication Site) ● Publication site (DOI): 10.1021/acsmedchemlett.9b00034 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1021/acsmedchemlett.9b00034 (DOI: 10.1021/acsmedchemlett.9b00034)
8.	FangFang Ma, Hiroki Takanari, Kimiko Masuda, Masaki Morishima and Katsushige Ono : Short- and long-term inhibition of cardiac inward-rectifier potassium channel current by an antiarrhythmic drug bepridil., Heart and Vessels, Vol.31, No.7, 1176-1184, 2016. (Summary) Bepridil is an effective antiarrhythmic drug on supraventricular and ventricular arrhythmias, and inhibitor of calmodulin. Recent investigations have been elucidating that bepridil exerts antiarrhythmic effects through its acute and chronic application for patients. The aim of this study was to identify the efficacy and the potential mechanism of bepridil on the inward-rectifier potassium channel in neonatal rat cardiomyocytes in acute- and long-term conditions. Bepridil inhibited inward-rectifier potassium current (I K1) as a short-term effect with IC50 of 17 μM. Bepridil also reduced I K1 of neonatal cardiomyocytes when applied for 24 h in the culture medium with IC50 of 2.7 μM. Both a calmodulin inhibitor (W-7) and an inhibitor of calmodulin-kinase II (KN93) reduced I K1 when applied for 24 h as a long-term effect in the same fashion, suggesting that the long-term application of bepridil inhibits I K1 more potently than that of the short-term application through the inhibition of calmodulin kinase II pathway in cardiomyocytes. (Keyword) Animals / Animals, Newborn / Anti-Arrhythmia Agents / Benzylamines / Bepridil / Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Calmodulin / Cells, Cultured / Membrane Potentials / Myocytes, Cardiac / Potassium Channel Blockers / Potassium Channels, Inwardly Rectifying / Protein Kinase Inhibitors / Rats, Wistar / Sulfonamides / Time Factors (Link to Publication Site) ● Publication site (DOI): 10.1007/s00380-015-0762-1 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26498939 ● Search Scopus @ Elsevier (PMID): 26498939 ● Search Scopus @ Elsevier (DOI): 10.1007/s00380-015-0762-1 (DOI: 10.1007/s00380-015-0762-1, PubMed: 26498939)
9.	Masaki Morishima, Eriko Iwata, Chisato Nakada, Yoshiyuki Tsukamoto, Hiroki Takanari, Shinji Miyamoto, Masatsugu Moriyama and Katsushige Ono : Atrial Fibrillation-Mediated Upregulation of miR-30d Regulates Myocardial Electrical Remodeling of the G-Protein-Gated K(+) Channel, IK.ACh., Circulation Journal, Vol.80, No.6, 1346-1355, 2016. (Summary) Atrial fibrillation (AF) begets AF in part due to atrial remodeling, the molecular mechanisms of which have not been completely elucidated. This study was conducted to identify microRNA(s) responsible for electrical remodeling in AF. The expression profiles of 1205 microRNAs, in cardiomyocytes from patients with persistent AF and from age-, gender-, and cardiac function-matched control patients with normal sinus rhythm, were examined by use of a microRNA microarray platform. Thirty-nine microRNAs differentially expressed in AF patients' atria were identified, including miR-30d, as a candidate responsible for ion channel remodeling by in silico analysis. MiR-30d was significantly upregulated in cardiomyocytes from AF patients, whereas the mRNA and protein levels ofCACNA1C/Cav1.2 andKCNJ3/Kir3.1, postulated targets of miR-30d, were markedly reduced.KCNJ3/Kir3.1 expression was downregulated by transfection of the miR-30 precursor, concomitant with a reduction of the acetylcholine-sensitive inward-rectifier K(+)current (IK.ACh).KCNJ3/Kir3.1 (but notCACNA1C/Cav1.2) expression was enhanced by the knockdown of miR-30d. The Ca(2+)ionophore, A23187, induced a dose-dependent upregulation of miR-30d, followed by the suppression ofKCNJ3mRNA expression. Blockade of protein kinase C signaling blunted the [Ca(2+)]i-dependent downregulation of Kir3.1 via miR-30d. The downward remodeling ofIK.AChis attributed, at least in part, to deranged Ca(2+)handling, leading to the upregulation of miR-30d in human AF, revealing a novel post-transcriptional regulation ofIK.ACh. (Circ J 2016; 80: 1346-1355). (Keyword) Aged / Atrial Fibrillation / Case-Control Studies / Cells, Cultured / Down-Regulation / female / GTP-Binding Proteins / Humans / male / MicroRNAs / Middle Aged / Myocytes, Cardiac / Potassium Channels, Inwardly Rectifying / Potassium Channels, Voltage-Gated / Up-Regulation (Tokushima University Institutional Repository) ● Metadata: 118215 (Link to Publication Site) ● Publication site (DOI): 10.1253/circj.CJ-15-1276 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27180889 ● Summary page in Scopus @ Elsevier: 2-s2.0-84969731123 (Tokushima University Institutional Repository: 118215, DOI: 10.1253/circj.CJ-15-1276, PubMed: 27180889, Elsevier: Scopus)

Academic Paper (Unrefereed Paper):

1.	Suzuki Akihiro, Kunihiro Otsuka, Hiroki Takanari, Kentaro Nagamatsu and Akihiro Shirai : 次世代光による細胞光応答の解明, LED総合フォーラム 2020 in 徳島論文集, Vol.P-17, 105-106, 2020.

Review, Commentary:

1.	NAYA Yuki and Hiroki Takanari : 画像処理・画像解析による医療支援 ∼内視鏡と画像強調解析における画像センサの役割∼(1)(2), センサイトWEBジャーナル 2022年6月号, Jun. 2022. (Tokushima University Institutional Repository) ● Metadata: 118293 (Tokushima University Institutional Repository: 118293)
2.	Hiroki Takanari and Taira Kajisa : 医光融合研究の最先端, OPTRONICS, Vol.39, No.465, 111-116, Sep. 2020. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520854805366131456 (CiNii: 1520854805366131456)

Proceeding of International Conference:

1.	MIKU Matsumoto, Hiroki Takanari, Yasuo Minami and Shin-ichiro Yanagiya : In-situ observation of photo-induced phenomena of AuNP-deposited HeLa cells with femto second laser, Program of pLED International Symposium 2023: Exploring Invisible Light Technology, P-26, Mar. 2023.
2.	Y Kanemura, Hiroki Takanari and T Mizuno : Assessment of actin fibers in the cell using fluorescent polarization microscopy., Focus on Microscopy 2020 (poster presentation), Osaka, Apr. 2020.
3.	Hiroki Takanari, Y Hayashi, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : Quantification of liver fibrosis by SHG microscopy., Focus on Microscopy 2020, Osaka, Apr. 2020.
4.	Eiji Hase, Hiroki Takanari, Mayuko Shimizu, Hayashi Yuri, Takeo Minamikawa, Takeshi Yasui and Koichi Tsuneyama : Characterization of fibrosis in non-alcoholic steatohepatitis by use of second-harmonic-generation microscopy, Asian Pacific Association for the Study of the Liver 2020 (APASL 2020), Bali, Indonesia, Mar. 2020.
5.	Takeo Minamikawa, Mayuko Shimizu, Hiroki Takanari, Shiomi Ryosuke, Kusaka Hiroki, Tanioka Hiroki, Eiji Hase, Takeshi Yasui and Koichi Tsuneyama : Molecular imaging analysis of accumulated fats in non-alcoholic steatohepatitis by Raman microscopy, Asian Pacific Association for the Study of the Liver 2020 (APASL 2020), Bali, Indonesia, Mar. 2020.

Proceeding of Domestic Conference:

1.	松本実久, Shin-ichiro Yanagiya, Hiroki Takanari and Yasuo Minami : Photo-induced phenomena of AuNP-deposited HeLa cells with fs laser, 第43回レーザー学会年次大会, P01-20p-P-27-P01-20p-P-27], Jan. 2023.
2.	Motoki Taniguchi, Takeshi Honda, Shin-ichiro Yanagiya, Hiroki Takanari, Yasuo Minami, Shingen Nakamura, Hirokazu Miki, Masahiro Abe, Yoshimi Bando and Koichi Tsuneyama : ALアミロイドーシス無染色標本のラマン分光顕微観察, 2022年第83回応用物理学会秋季学術講演会, 20p-C301-12, Sep. 2022. (Keyword) Raman spectroscopy / amyloidosis
3.	Takeo Minamikawa, Mayuko Shimizu, Hiroki Tanioka, Eiji Hase, Yuki Morimoto, Hiroki Takanari, Takeshi Yasui and Koichi Tsuneyama : ラマン散乱分光法を用いた非アルコール性脂肪性肝疾患の脂質分子構造イメージング解析, レーザ顕微鏡研究会第46回講演会, Nov. 2021.
4.	Hiroki Tanioka, Takeo Minamikawa, Mayuko Shimizu, Yuki Morimoto, Hiroki Takanari, Yutaro Onishi, Eiji Hase, Takeshi Yasui and Koichi Tsuneyama : 表面増強ラマン散乱分光法を用いた非アルコール性脂肪肝疾患モデルの高感度ラマンイメージング, 第82回応用物理学会秋季学術講演会予稿集, 13a-N202-9, Sep. 2021.
5.	Yuki Naya and Hiroki Takanari : 血管強調画像観察に用いる光源の検証, 第98回日本生理学会・第126回日本解剖学会合同大会, Mar. 2021.
6.	Midori Ueta, Hiroki Takanari and Koichi Tsuneyama : ラマン分光法による肺癌組織型の判別, 第98回日本生理学会・第126回日本解剖学会合同大会, Mar. 2021.
7.	Hiroki Tanioka, Takeo Minamikawa, Mayuko Shimizu, Yuki Morimoto, Hiroki Takanari, Ryosuke Shiomi, Eiji Hase, Takeshi Yasui and Koichi Tsuneyama : 非アルコール性脂肪性肝炎診断に対するラマン顕微鏡法の適用可能性の検証計, 第68回応用物理学会春季学術講演会予稿集, 19a-Z04-9, Mar. 2021.
8.	Kazuma Kusumoto, Hiroki Takanari, Atsushi Tabata, Hideaki Nagamune, Yukihiro Arakawa, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : D-π-A型ベンゾチアゾール誘導体の合成と光機能性, 2020年日本化学会中国四国支部大会島根大会, Nov. 2020.
9.	Yuki Naya and Hiroki Takanari : Narrow Band Imaging (NBI) を用いた炎症部位の特定, 生体医工学シンポジウム2020, Sep. 2020.
10.	Kazuma Kusumoto, Yohei Sanagawa, Eiji Hase, Hiroki Takanari, Atsushi Tabata, Hideaki Nagamune, Yukihiro Arakawa, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : 多光子励起を目指したA-D-A型N-へテロアレーン類のデザインと応用, 2020年web光化学討論会, Sep. 2020.
11.	Ryuta Umebayashi, Masaki Okamoto, Eiji Hase, Hiroki Takanari, Atsushi Tabata, Hideaki Nagamune, Yukihiro Arakawa, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : 多光子励起可能な水溶性イミダゾ[1,2-a]ピリジニウム塩の合成とミトコンドリアイメージング, 2020年web光化学討論会, Sep. 2020.
12.	Yuki Naya, Hiroki Takanari and Taira Kajisa : 毛細血管の可視化を目指したNarrow Band Imaging(NBI)の光源特性の検証, 第81回日本応用物理学会秋季学術講演会, Sep. 2020.
13.	Fumitoshi Yagishita, Junichi Tanigawa, Masaki Okamoto, Eiji Hase, Hiroki Takanari, Atsushi Tabata, Hideaki Nagamune, Takeo Minamikawa, Takeshi Yasui, Yasushi Imada and Yasuhiko Kawamura : Synthesis of Imidazopyridinium Salts Having Potential Application as Image-guided Photodynamic Therapy Agents, The 100th CSJ Annual Meeting, Mar. 2020.
14.	Fumitoshi Yagishita, Masaki Okamoto, Hiroki Takanari, Eiji Hase, Atsushi Tabata, Hideaki Nagamune, Yasushi Imada, Takeshi Yasui and Yasuhiko Kawamura : ミトコンドリアイメージングを可能とするイミダゾ[1,2-a]ピリジニウム塩の合成, 2019年日本化学会中国四国支部大会徳島大会, Nov. 2019.
15.	Fumitoshi Yagishita, Junichi Tanigawa, Chiho Nii, Atsushi Tabata, Hideaki Nagamune, Hiroki Takanari, Yasushi Imada and Yasuhiko Kawamura : 光線力学療法への応用を狙いとしたイミダゾ[1,5-a]ピリジニウム塩の合成, 2019年日本化学会中国四国支部大会徳島大会, Nov. 2019.
16.	Fumitoshi Yagishita, Ryuta Umebayashi, Masaki Okamoto, Atsushi Tabata, Hideaki Nagamune, Hiroki Takanari, Yasushi Imada and Yasuhiko Kawamura : D-π-A 型イミダゾ [1,2-a] ピリジニウム塩の合成とミトコンドリアイメージングへの応用, 2019年光化学討論会, Sep. 2019.
17.	Kenzo Yamaguchi, Eiji Hase, Yohhei Kanemura, Hiroki Takanari, Toshihiro Okamoto, Masanobu Haraguchi and Takeshi Yasui : 金ナノ粒子を用いたバイオイメージングにおけるコントラストの増強, 生体医工学シンポジウム2019, 2A-30, Sep. 2019.

Et cetera, Workshop:

1.	Hiroki Tanioka, Takeo Minamikawa, Mayuko Shimizu, 森本友樹, Hiroki Takanari, Ryosuke Shiomi, Eiji Hase, Takeshi Yasui and Koichi Tsuneyama : 非アルコール性脂肪肝炎の蓄積した脂肪に着目したラマン分光イメージング, LED総合フォーラム2021 in 徳島, P-14, Feb. 2021.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

光誘起現象を制御した複合ナノ結晶材料の設計と医光応用 (Project/Area Number: 22K04713 )
Development of Contactless and Label-free Measurement of Membrane Potential Using Dual-Comb Spectroscopy (Project/Area Number: 21K19913 )
miRNA発現パターンと病理所見に基づく機械学習による冠動脈硬化症のクラスター化 (Project/Area Number: 21K10533 )
Novel and rapid diagnostic appproach of AL amyloidosis by Raman spectroscopy (Project/Area Number: 20K08712 )
Development of magnetic resonance Raman spectrometry for application of optical technology to living body (Project/Area Number: 19H04443 )
Alcohol dehydrogenase in alcohol-related organ disorder (Project/Area Number: 19H04038 )
Proof of concept study of a novel excess chromosome elimination therapy to ameliorate and prevent trisomy 21 associated complications (Project/Area Number: 16K09964 )
The mechanism of pericardial rupture associated with cardiopulmonary resuscitation involving chest compression (Project/Area Number: 16K09222 )
A new technology for tissue diagnosis with combination of Raman scattering spectrometry and bioimpedance measurement (Project/Area Number: 16H05238 )
Abnormal transcriptional regulation in Cockayne syndrome and its effect on neuronal differentiation (Project/Area Number: 15K06758 )
Effects of fetal gene reprogramming on expression, distribution and expression of gap junction in failing hearts (Project/Area Number: 26860215 )
Myocardial regeneration research based on cell-cycle progression of the adult myocyte (Project/Area Number: 26670189 )
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