Tokushima University / Educator and Researcher Directory --- Hase, Eiji

Personal Web Page

https://researchmap.jp/ehase (researchmap)

https://orcid.org/0000-0002-1414-9610 (ORCID)

Field of Study

○	Nanotechnology/Materials [Optical engineering and photonics]
○	Life sciences [Biomedical engineering]

Subject of Study

Book / Paper

Book:

1.	小倉有紀, Takeshi Yasui and Eiji Hase : 5.5 第二高調波発生(SHG)光によるコラーゲン観察, MARUZEN Co.,Ltd., Feb. 2021.

Academic Paper (Judged Full Paper):

1.	Saya Matsuzaki, Eiji Hase, Hiroki Takanari, Yuri Hayashi, Yusaku Hayashi, Haruto Oshikata, Takeo Minamikawa, Satoko Kimura, Mayuko Ichimura-Shimizu, Takeshi Yasui, Masafumi Harada and Koichi Tsuneyama : Quantification of collagen fiber properties in alcoholic liver fibrosis using polarization-resolved second harmonic generation microscopy., Scientific reports, Vol.13, No.1, 2023. (Summary) Liver fibrosis is assessed mainly by conventional staining or second harmonic generation (SHG) microscopy, which can only provide collagen content in fibrotic area. We propose to use polarization-resolved SHG (PR-SHG) microscopy to quantify liver fibrosis in terms of collagen fiber orientation and crystallization. Liver samples obtained from autopsy cases with fibrosis stage of F0-F4 were evaluated with an SHG microscope, and 12 consecutive PR-SHG images were acquired while changing the polarization azimuth angle of the irradiated laser from 0° to 165° in 15° increments using polarizer. The fiber orientation angle (φ) and degree (ρ) of collagen were estimated from the images. The SHG-positive area increased as the fibrosis stage progressed, which was well consistent with Sirius Red staining. The value of φ was random regardless of fibrosis stage. The mean value of ρ (ρ-mean), which represents collagen fiber crystallinity, varied more as fibrosis progressed to stage F3, and converged to a significantly higher value in F4 than in other stages. Spatial dispersion of ρ (ρ-entropy) also showed increased variation in the stage F3 and decreased variation in the stage F4. It was shown that PR-SHG could provide new information on the properties of collagen fibers in human liver fibrosis. (Keyword) Humans / Second Harmonic Generation Microscopy / Collagen / Liver Cirrhosis / Refraction, Ocular / Coloring Agents (Link to Publication Site) ● Publication site (DOI): 10.1038/s41598-023-48887-8 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 38092851 ● Search Scopus @ Elsevier (PMID): 38092851 ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-023-48887-8 (DOI: 10.1038/s41598-023-48887-8, PubMed: 38092851)
2.	Eiji Hase, Yu Tokizane, Kazuki Sadahiro, Takeo Minamikawa, Isao Morohashi and Takeshi Yasui : Nanometer-precision surface metrology of millimeter-sized stepped objects using full-cascade-linked synthetic-wavelength digital holography using a line-by-line full-mode-extracted optical frequency comb, Optics Express, Vol.31, No.11, 18088-18097, 2023. (Summary) Digital holography (DH) is a powerful tool for the surface profilometry of objects with sub-wavelength precision. In this article, we demonstrate full-cascade-linked synthetic-wavelength DH for nanometer-precision surface metrology of millimeter-sized stepped objects. 300 modes of optical frequency comb (OFC) with different wavelengths are sequentially extracted at a step of mode spacing from a 10GHz-spacing, 3.72THz-spanning electro-optic modulator OFC. The resulting 299 synthetic wavelengths and a single optical wavelength are used to generate a fine-step wide-range cascade link covering within a wavelength range of 1.54 µm to 29.7 mm. We determine the sub-millimeter and millimeter step differences with axial uncertainty of 6.1 nm within the maximum axial range of 14.85 mm. (Link to Publication Site) ● Publication site (DOI): 10.1364/OE.483408 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37381527 ● Search Scopus @ Elsevier (PMID): 37381527 ● Search Scopus @ Elsevier (DOI): 10.1364/OE.483408 (DOI: 10.1364/OE.483408, PubMed: 37381527)
3.	Yu Tokizane, Shota Okada, Kenji Nishimoto, Yasuhiro Okamura, Hiroki Kishikawa, Takeo Minamikawa, Eiji Hase, Junichi Fujikata, Masanobu Haraguchi, Atsushi Kanno, Shintaro Hisatake, Naoya Kuse and Takeshi Yasui : Terahertz wireless communication in a 560-GHz band using a Kerr micro-resonator soliton comb, Optics Continuum, Vol.2, No.5, 1267-1275, 2023. (Tokushima University Institutional Repository) ● Metadata: 118500 (Link to Publication Site) ● Publication site (DOI): 10.1364/OPTCON.491729 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1364/OPTCON.491729 (Tokushima University Institutional Repository: 118500, DOI: 10.1364/OPTCON.491729)
4.	Takeo Minamikawa, Eiji Hase, Mayuko Shimizu, Yuki Morimoto, Akihiro Suzuki, Takeshi Yasui, Satoko Nakamura, Akemi Tsutsui, Koichi Takaguchi and Koichi Tsuneyama : Assessment of Ultra-Early-Stage Liver Fibrosis in Human Non-Alcoholic Fatty Liver Disease by Second-Harmonic Generation Microscopy., International Journal of Molecular Sciences, Vol.23, No.6, 3357, 2022. (Summary) Non-alcoholic fatty liver disease (NAFLD) is associated with the chronic progression of fibrosis. In general, the progression of liver fibrosis is determined by a histopathological assessment with a collagen-stained section; however, the ultra-early stage of liver fibrosis is challenging to identify because of the low sensitivity in the collagen-selective staining method. In the present study, we demonstrate the feasibility of second-harmonic generation (SHG) microscopy in the histopathological diagnosis of the liver of NAFLD patients for the quantitative assessment of the ultra-early stage of fibrosis. We investigated four representative NAFLD patients with early stages of fibrosis. SHG microscopy visualised well-matured fibrotic structures and early fibrosis diffusely involving liver tissues, whereas early fibrosis is challenging to be identified by conventional histopathological methods. Furthermore, the SHG emission directionality analysis revealed the maturation of each collagen fibre of each patient. As a result, SHG microscopy is feasible for assessing liver fibrosis on NAFLD patients, including the ultra-early stage of liver fibrosis that is difficult to diagnose by the conventional histopathological method. The assessment method of the ultra-early fibrosis by using SHG microscopy may serve as a crucial means for pathological, clinical, and prognostic diagnosis of NAFLD patients. (Keyword) Biopsy / Collagen / Fibrosis / Humans / Liver / Liver Cirrhosis / Non-alcoholic Fatty Liver Disease / Second Harmonic Generation Microscopy (Tokushima University Institutional Repository) ● Metadata: 117221 (Link to Publication Site) ● Publication site (DOI): 10.3390/ijms23063357 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35328778 ● Summary page in Scopus @ Elsevier: 2-s2.0-85126575411 (Tokushima University Institutional Repository: 117221, DOI: 10.3390/ijms23063357, PubMed: 35328778, Elsevier: Scopus)
5.	Hidenori Koresawa, Kohta Seki, Eiji Hase, Yu Tokizane, Takeo Minamikawa, Taka-aki Yano, Taira Kajisa and Takeshi Yasui : Beam-angle-scanning surface plasmon resonance sensor for rapid, high-precision sensing of refractive index and bio-molecules, Optics Continuum, Vol.1, No.3, 565-574, 2022. (Link to Publication Site) ● Publication site (DOI): 10.1364/OPTCON.451947 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1364/OPTCON.451947 (DOI: 10.1364/OPTCON.451947)
6.	Yu Tokizane, Takayoshi Yamaguchi, Takeo Minamikawa, Eiji Hase, Kenzo Yamaguchi, Akihiro Suzuki, Takao Ueda and Takeshi Yasui : Ultralow-frequency ultranarrow-bandwidth coherent terahertz imaging for nondestructive testing of mortar material, Optics Express, Vol.30, No.3, 4392-4401, 2022. (Summary) Nondestructive testing of concrete materials is essential in civil engineering to maintain social infrastructure such as buildings or bridges. In this study, we constructed an ultralow-frequency, ultranarrow-bandwidth, coherent terahertz (THz) imaging system based on THz time-domain spectroscopy (THz-TDS). Based on its ultralow-frequency-localized THz wave and coherent detection, the present system achieved a wide dynamic range of THz power over 100 dB at 0.046 THz, which is appropriate to measure the mortar material. The achieved dynamic range of the THz power was 59 dB larger than that of a commercialized THz-TDS system and 49 dB larger than that of an ultralow-frequency noncoherent THz imaging system equipped with a high-power electric THz source. Ultimately, the proposed system could visualize the inner structure of a mortar sample with a thickness of 10 mm, and the present system can investigate a mortar sample with a thickness of over 130 mm. The proposed method is an attractive tool for non-destructive testing of thick concrete structures characterized by non-invasiveness and non-contact remoteness. (Link to Publication Site) ● Publication site (DOI): 10.1364/OE.449092 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35209677 ● Search Scopus @ Elsevier (PMID): 35209677 ● Search Scopus @ Elsevier (DOI): 10.1364/OE.449092 (DOI: 10.1364/OE.449092, PubMed: 35209677)
7.	Keita Hoshi, Kazuma Kusumoto, Airi Matsumoto, Atsushi Tabata, Hideaki Nagamune, Eiji Hase, Takeo Minamikawa, Takeshi Yasui, Yasushi Yoshida, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : Synthesis of D-π-A type benzothiazolepyridinium salt composite and its application as photo-degradation agent for amyloid fibrils, Bioorganic & Medicinal Chemistry Letters, Vol.50, 128324, 2021. (Summary) We have synthesized a cyan fluorescent benzothiazole-pyridinium salt composite based on D-p-A architecture. This salt was found to work as not only a two- and three-photon excitable fluorophore but also a degradation agent against amyloid fibrils under LED irradiation conditions. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bmcl.2021.128324 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34403727 ● Search Scopus @ Elsevier (PMID): 34403727 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bmcl.2021.128324 (DOI: 10.1016/j.bmcl.2021.128324, PubMed: 34403727)
8.	Keita Hoshi, Masami Itaya, Koki Tahara, Airi Matsumoto, Atsushi Tabata, Hideaki Nagamune, Yasushi Yoshida, Eiji Hase, Takeo Minamikawa, Takeshi Yasui, Tetsuro Katayama, Akihiro Furube, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : Two-photon excitable boron complex based on tridentate imidazo[1,5-a]pyridine ligand for heavy- atom-free mitochondria-targeted photodynamic therapy, RSC Advances, Vol.11, 26403-26407, 2021. (Tokushima University Institutional Repository) ● Metadata: 116625 (Link to Publication Site) ● Publication site (DOI): 10.1039/d1ra05059a (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1039/d1ra05059a (Tokushima University Institutional Repository: 116625, DOI: 10.1039/d1ra05059a)
9.	Eiji Hase, Yu Tokizane, Masatomo Yamagiwa, Takeo Minamikawa, Hirotsugu Yamamoto, Morohashi Isao and Takeshi Yasui : Multicascade-linked synthetic-wavelength digital holography using a line-by-line spectral-shaped optical frequency comb, Optics Express, Vol.29, No.10, 15772-15785, 2021. (Link to Publication Site) ● Publication site (DOI): 10.1364/OE.424458 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1364/OE.424458 (DOI: 10.1364/OE.424458)
10.	Eiji Hase, Takeo Minamikawa, Katsuya SATO, Daisuke Yonekura, Mitsuhiko Takahashi and Takeshi Yasui : Quantitative evaluation of both histological and mechanical recovery in injured tendons using Fourier-transform second-harmonic-generation microscopy, IEEE Journal of Selected Topics in Quantum Electronics, Vol.27, No.4, 6801608, 2021. (Link to Publication Site) ● Publication site (DOI): 10.1109/JSTQE.2021.3063535 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1109/JSTQE.2021.3063535 (DOI: 10.1109/JSTQE.2021.3063535)
11.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Yu Tokizane, Ryo Oe, Hidenori Koresawa, Hirotsugu Yamamoto and Takeshi Yasui : Full-field fluorescence lifetime dual-comb microscopy using spectral mapping and frequency multiplexing of dual-comb optical beats, Science Advances, Vol.7, No.1, eabd2102, 2021. (Summary) Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust to photobleaching. However, since most FLIMs are based on point-to-point measurements, mechanical scanning of a focal spot is needed for forming an image, which hampers rapid imaging. Here, we demonstrate scan-less full-field FLIM based on a one-to-one correspondence between two-dimensional (2D) image pixels and frequency-multiplexed radio frequency (RF) signals. A vast number of dual-comb optical beats between dual optical frequency combs are effectively adopted for 2D spectral mapping and high-density frequency multiplexing in the RF region. Bimodal images of fluorescence amplitude and lifetime are obtained with high quantitativeness from amplitude and phase spectra of fluorescence RF comb modes without the need for mechanical scanning. The parallelized FLIM will be useful for rapid quantitative fluorescence imaging in life science. (Tokushima University Institutional Repository) ● Metadata: 116627 (Link to Publication Site) ● Publication site (DOI): 10.1126/sciadv.abd2102 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33523842 ● Search Scopus @ Elsevier (PMID): 33523842 ● Search Scopus @ Elsevier (DOI): 10.1126/sciadv.abd2102 (Tokushima University Institutional Repository: 116627, DOI: 10.1126/sciadv.abd2102, PubMed: 33523842)
12.	Takeo Minamikawa, Mayuko Shimizu, Hiroki Takanari, Yuki Morimoto, Ryosuke Shiomi, Tanioka Hiroki, Eiji Hase, Takeshi Yasui and Koichi Tsuneyama : Molecular imaging analysis of microvesicular and macrovesicular lipid droplets in non-alcoholic fatty liver disease by Raman microscopy., Scientific Reports, Vol.10, No.1, 18548, 2020. (Summary) Predominant evidence of non-alcoholic fatty liver disease (NAFLD) is the accumulation of excess lipids in the liver. A small group with NAFLD may have a more serious condition named non-alcoholic steatohepatitis (NASH). However, there is a lack of investigation of the accumulated lipids with spatial and molecular information. Raman microscopy has the potential to characterise molecular species and structures of lipids based on molecular vibration and can achieve high spatial resolution at the organelle level. In this study, we aim to demonstrate the feasibility of Raman microscopy for the investigation of NAFLD based on the molecular features of accumulated lipids. By applying the Raman microscopy to the liver of the NASH model mice, we succeeded in visualising the distribution of lipid droplets (LDs) in hepatocytes. The detailed analysis of Raman spectra revealed the difference of molecular structural features of the LDs, such as the degree of saturation of lipids in the LDs. We also found that the inhomogeneous distribution of cholesterol in the LDs depending on the histology of lipid accumulation. We visualised and characterised the lipids of NASH model mice by Raman microscopy at organelle level. Our findings demonstrated that the Raman imaging analysis was feasible to characterise the NAFLD in terms of the molecular species and structures of lipids. (Tokushima University Institutional Repository) ● Metadata: 115619 (Link to Publication Site) ● Publication site (DOI): 10.1038/s41598-020-75604-6 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33122711 ● Search Scopus @ Elsevier (PMID): 33122711 ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-020-75604-6 (Tokushima University Institutional Repository: 115619, DOI: 10.1038/s41598-020-75604-6, PubMed: 33122711)
13.	Keita Hoshi, Yohei Sanagawa, Ryuta Umebayashi, Atsushi Tabata, Hideaki Nagamune, Eiji Hase, Takeo Minamikawa, Takeshi Yasui, Yasushi Yoshida, Keiji Minagawa, Yasuhiko Kawamura, Yasushi Imada and Fumitoshi Yagishita : Synthesis and Optical Property of Quadrupolar Pyridinium Salt and Its Application as Bioimaging Agent, Chemistry Letters, Vol.49, No.12, 1487-1489, 2020. (Link to Publication Site) ● Publication site (DOI): 10.1246/cl.200604 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1246/cl.200604 (DOI: 10.1246/cl.200604)
14.	Eiji Hase, Hiroki Takanari, Keita Hoshi, Masaki Okamoto, Atsushi Tabata, Hideaki Nagamune, Takeo Minamikawa, Takeshi Yasui, Yasushi Yoshida, Keiji Minagawa, Yasuhiko Kawamura, Yasushi Imada and Fumitoshi Yagishita : Two- and three-photon excitable quaternized imidazo[1,2-a]pyridines for mitochondrial imaging and potent cancer therapy agent, Organic & Biomolecular Chemistry, Vol.18, No.38, 7571-7576, 2020. (Link to Publication Site) ● Publication site (DOI): 10.1039/D0OB01585G (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32940324 ● Search Scopus @ Elsevier (PMID): 32940324 ● Search Scopus @ Elsevier (DOI): 10.1039/D0OB01585G (DOI: 10.1039/D0OB01585G, PubMed: 32940324)
15.	Hidenori Koresawa, Marc Gouryeb, Kyuki Shibuya, Takahiko Mizuno, Eiji Hase, Yu Tokizane, Ryo Oe, Takeo Minamikawa and Takeshi Yasui : Dynamic characterization of polarization property in liquid-crystal-on-silicon spatial light modulator using dual-comb spectroscopic polarimetry, Optics Express, Vol.28, No.16, 23584-23593, 2020. (Summary) Spectroscopic polarimetry (SP) is a powerful tool for characterization of thin film, polarization optics, semiconductor, and others. However, mechanical polarization modulation of broadband light hampers its application for dynamic monitoring of a sample. In this article, we demonstrate the dynamic SP with features of polarization-modulation-free polarimetry and spectrometer-free spectroscopy benefiting from dual-comb spectroscopy (DCS) using a pair of optical frequency combs (OFCs). DCS enables the direct determination of polarization without the need for polarization modulation by using mode-resolved OFC spectra of amplitude and phase for two orthogonally linear-polarized lights while securing rapid, high-precision, broadband spectroscopy without the need for spectrometer. Effectiveness of the proposed system is highlighted by visualizing the hysteresis property of dynamic response in a liquid-crystal-on-silicon spatial light modulator at a sampling rate of 105 Hz. (Link to Publication Site) ● Publication site (DOI): 10.1364/OE.399200 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1364/OE.399200 (DOI: 10.1364/OE.399200)
16.	Takahiko Mizuno, Takuya Tsuda, Eiji Hase, Yu Tokizane, Ryo Oe, Hidenori Koresawa, Hirotsugu Yamamoto, Takeo Minamikawa and Takeshi Yasui : Optical image amplification in dual-comb microscopy, Scientific Reports, Vol.10, No.1, 8338, 2020. (Summary) Dual-comb microscopy (DCM), based on a combination of dual-comb spectroscopy (DCS) with two-dimensional spectral encoding (2D-SE), is a promising method for scan-less confocal laser microscopy giving an amplitude and phase image contrast with the confocality. However, signal loss in a 2D-SE optical system hampers increase in image acquisition rate due to decreased signal-to-noise ratio. In this article, we demonstrated optical image amplification in DCM with an erbium-doped fiber amplifier (EDFA). Combined use of the image-encoded DCS interferogram and the EDFA benefits from not only the batch amplification of amplitude and phase images but also significant rejection of amplified spontaneous emission (ASE) background. Effectiveness of the optical-image-amplified DCM is highlighted in the single-shot quantitative nanometer-order surface topography and the real-time movie of polystyrene beads dynamics under water convection. The proposed method will be a powerful tool for real-time observation of surface topography and fast dynamic phenomena. (Tokushima University Institutional Repository) ● Metadata: 115569 (Link to Publication Site) ● Publication site (DOI): 10.1038/s41598-020-64927-z (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32433533 ● Search Scopus @ Elsevier (PMID): 32433533 ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-020-64927-z (Tokushima University Institutional Repository: 115569, DOI: 10.1038/s41598-020-64927-z, PubMed: 32433533)
17.	Tian Tian, Cai Bin, Cheng Qingqing, Fan Cheng, Wang Yanyan, Xu Gongjie, Gu Fuxing, Liao Feng, Sugihara Okihiro, Eiji Hase and Takeshi Yasui : One-drop self-assembly of ultra-fine second-order organic nonlinear optical crystal nanowires, Nanoscale Research Letters, Vol.14, No.1, 269, 2019. (Summary) In this study, we propose a one-drop self-assembly method, which proved capable of successfully preparing 4-N, N-dimethylamino-4'-N'-methyl-stilbazolium tosylate (DAST) single-crystalline nanowires (NWs). The apparent roughness of the DAST NWs was determined to be less than 100 pm by using a high-resolution atomic force microscope, indicating their ultrafine quality. The DAST NWs also exhibited excellent nonlinear optical properties, including two-photon excited fluorescence and second harmonic generation, which could enable the production of low-cost, low-power-consumption wideband wavelength conversion devices. Thus, the described method may provide a new avenue for organic NW fabrication. (Tokushima University Institutional Repository) ● Metadata: 115609 (Link to Publication Site) ● Publication site (DOI): 10.1186/s11671-019-3103-y (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31392425 ● Search Scopus @ Elsevier (PMID): 31392425 ● Search Scopus @ Elsevier (DOI): 10.1186/s11671-019-3103-y (Tokushima University Institutional Repository: 115609, DOI: 10.1186/s11671-019-3103-y, PubMed: 31392425)
18.	Takeo Minamikawa, Takashi Masuoka, Takashi Ogura, Kyuki Shibuya, Ryo Oe, Eiji Hase, Yoshiaki Nakajima, Yoshihisa Yamaoka, Takahiko Mizuno, Masatomo Yamagiwa, Yasuhiro Mizutani, Hirotsugu Yamamoto, Tetsuo Iwata, Kaoru Minoshima and Takeshi Yasui : Ultrasonic wave sensing using an optical-frequency-comb sensing cavity for photoacoustic imaging, OSA Continuum, Vol.2, No.2, 439-449, 2019. (Summary) We propose a novel ultrasonic wave sensing method employing an optical-frequency-comb (OFC) cavity, namely, an OFC sensing cavity-based ultrasonic wave sensor. We confirm the fundamental characteristics of the OFC sensing cavity-based ultrasonic wave sensor by using an ultrasonic wave transducer and realize the frequency response of up to 13 MHz with a linear response. We also confirm the potential applicability in photoacoustic imaging employing an 8-ns pulsed laser. Our demonstrations confirm the capability of the concept of the OFC sensing cavity in ultrasonic wave sensing and will also expand the potential of OFC technology. (Link to Publication Site) ● Publication site (DOI): 10.1364/OSAC.2.000439 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1364/OSAC.2.000439 (DOI: 10.1364/OSAC.2.000439)
19.	Katsuya SATO, Oki Matsubara, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : Quantitative in situ time-series evaluation of osteoblastic collagen synthesis under cyclic strain using second-harmonic-generation microscopy, Journal of Biomedical Optics, Vol.24, No.3, 031019, 2019. (Summary) The aim of this study is to provide an in situ method to non-invasively monitor osteoblastic collagen synthesis under mechanical stimulation. We applied second-harmonic-generation (SHG) microscopy to monitor the collagen fibers produced by osteoblast-like cells. To evaluate the influence of mechanical stimulation on collagen synthesis and maturation, we compared SHG images of osteoblast-produced collagen fibers with and without a cyclic stretch stimulus. Image analysis of the average SHG intensity indicated that the amount of osteoblastic collagen synthesis was significantly enhanced by the cyclic stretch. Furthermore, the maturity of the collagen fibers was not affected in the early stage of bone formation by the mechanical stimulus.© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only. (Keyword) Collagen / Humans / Image Interpretation, Computer-Assisted / Microscopy / Stress, Mechanical (Link to Publication Site) ● Publication site (DOI): 10.1117/1.JBO.24.3.031019 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30635995 ● Search Scopus @ Elsevier (PMID): 30635995 ● Search Scopus @ Elsevier (DOI): 10.1117/1.JBO.24.3.031019 (DOI: 10.1117/1.JBO.24.3.031019, PubMed: 30635995)
20.	Eiji Hase, Takeo Minamikawa, Shuji Miyamoto, Ryuji Ichikawa, Yi-Da Hsieh, Yasuhiro Mizutani, Tetsuo Iwata, Hirotsugu Yamamoto and Takeshi Yasui : Scan-Less, Kilo-Pixel, Line-Field Confocal Phase Imaging with Spectrally Encoded Dual-Comb Microscopy, IEEE Journal of Selected Topics in Quantum Electronics, Vol.25, No.1, 6801408, 2018. (Summary) Confocal laser microscopy (CLM) is a powerful tool in life science research and industrial inspection, and its image acquisition rate is boosted by scan-less imaging techniques. However, the optical-intensity-based image contrast in CLM makes it difficult to visualize transparent non-fluorescent objects or reflective objects with nanometer unevenness. In this paper, we introduce an optical frequency comb (OFC) to scan-less CLM to give the optical-phase-based image contrast. One-dimensional (1D) image pixels of a sample are separately encoded onto OFC modes via 1D spectral encoding by using OFC as an optical carrier of amplitude and phase with a vast number of discrete frequency channels. Then, line-field confocal information of amplitude and phase are decoded from a mode-resolved OFC amplitude and phase spectra obtained by dual-comb spectroscopy without the need for mechanical scanning. The proposed confocal phase imaging will further expand the application fields of CLM. (Tokushima University Institutional Repository) ● Metadata: 113637 (Link to Publication Site) ● Publication site (DOI): 10.1109/JSTQE.2018.2879017 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85056348979 (Tokushima University Institutional Repository: 113637, DOI: 10.1109/JSTQE.2018.2879017, Elsevier: Scopus)
21.	Yuki Ogura, Kosuke Atsuta, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : Photonic-Crystal-Fiber-Coupled, Hand-Held, Polarization-Resolved Second-Harmonic-Generation Microscope for In Vivo Visualization of Dermal Collagen Fibers in Human Skin, IEEE Journal of Selected Topics in Quantum Electronics, Vol.25, No.1, 6801007, 2018. (Summary) Second-harmonic-generation (SHG) microscopy is a powerful tool for in vivo monitoring of collagen fibers in human skin. However, its practical use in the dermatological field is still limited due to the bulky and complicated setup. In this paper, we constructed a photonic-crystal-fiber-coupled, hand-held polarization-resolved SHG microscope for in vivo monitoring of collagen fibers in human skin. Fiber delivery of ultrashort pulse light was achieved by a large-mode-area photonic-crystal-fiber whereas the SHG microscopy setup was enclosed into a hand-held probe head. The combination of PCF with the hand-held probe head largely enhances the flexibility of measurement sites in the human skin. (Tokushima University Institutional Repository) ● Metadata: 113639 (Link to Publication Site) ● Publication site (DOI): 10.1109/JSTQE.2018.2865779 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85051753929 (Tokushima University Institutional Repository: 113639, DOI: 10.1109/JSTQE.2018.2865779, Elsevier: Scopus)
22.	Eiji Hase, Takeo Minamikawa, Takahiko Mizuno, Shuji Miyamoto, Ryuji Ichikawa, Yi-Da Hsieh, Kyuki Shibuya, Katsuya SATO, Yoshiaki Nakajima, Akifumi Asahara, Kaoru Minoshima, Yasuhiro Mizutani, Tetsuo Iwata, Hirotsugu Yamamoto and Takeshi Yasui : Scan-less confocal phase imaging based on dual-comb microscopy, Optica, Vol.5, No.5, 634-643, 2018. (Summary) Confocal laser microscopy (CLM) is a powerful tool in life science research and industrial inspection because it offers two-dimensional optical sectioning or three-dimensional imaging capability with micrometer depth selectivity. Furthermore, scan-less imaging modality enables rapid image acquisition and high robustness against surrounding external disturbances in CLM. However, the objects to be measured must be reflective, absorptive, scattering, or fluorescent because the image contrast is given by the optical intensity. If a new image contrast can be provided by the optical phase, scan-less CLM can be further applied for transparent non-fluorescent objects or reflective objects with nanometer unevenness by providing information on refractive index, optical thickness, or geometrical shape. Here, we report scan-less confocal dual-comb microscopy offering a phase image in addition to an amplitude image with depth selectivity by using an optical frequency comb as an optical carrier of amplitude and phase with discrete ultra-multichannels. Our technique encodes confocal amplitude and phase images of a sample onto a series of discrete modes in the optical frequency comb with well-defined amplitude and phase to establish a one-to-one correspondence between image pixels and comb modes. The technique then decodes these images from comb modes with amplitude and phase. We demonstrate confocal phase imaging with milliradian phase resolution under micrometer depth selectivity on the millisecond timescale. As a proof of concept, we demonstrate the quantitative phase imaging of standing culture fixed cells and the surface topography of nanometer-scale step structures. Our technique for confocal phase imaging will find applications in three-dimensional visualization of stacked living cells in culture and nanometer surface topography of semiconductor objects. (Tokushima University Institutional Repository) ● Metadata: 113646 (Link to Publication Site) ● Publication site (DOI): 10.1364/OPTICA.5.000634 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85047275172 (Tokushima University Institutional Repository: 113646, DOI: 10.1364/OPTICA.5.000634, Elsevier: Scopus)
23.	Eiji Hase, Takeo Minamikawa, Shuji Miyamoto, Yasuhiro Mizutani, Tetsuo Iwata, Hirotsugu Yamamoto and Takeshi Yasui : Application of Scan-less Two-Dimensional Confocal Microscopy Based on a Combination of Confocal Slit With Wavelength/Space Conversion, IEEE Journal of Selected Topics in Quantum Electronics, Vol.25, No.1, 7101407, 2018. (Summary) Confocal laser microscope (CLM) has been widely used in the fields of the non-contact surface topography, biomedical imaging, and other applications, because the confocality gives two-dimensional (2D) optical-sectioning or three-dimensional (3D) imaging capability with the depth selectivity. Combination of line-focused CLM with one-dimensional (1D) spectral encoding CLM enables us to obtain the 2D confocal image without the need for the mechanical scanning. So-called scan-less 2D CLM is a unique imaging modality, however, there are no attempts to apply for practical application. In this paper, we constructed scan-less 2D CLM with the image acquisition time of 0.23 ms, the lateral resolution of 1.2 μm, the depth resolution of 2.4 μm, and apply it for different kinds of application to evaluate its practical potential. (Tokushima University Institutional Repository) ● Metadata: 113638 (Link to Publication Site) ● Publication site (DOI): 10.1109/JSTQE.2018.2869615 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85053132099 (Tokushima University Institutional Repository: 113638, DOI: 10.1109/JSTQE.2018.2869615, Elsevier: Scopus)
24.	Takeo Minamikawa, Takashi Ogura, Yoshiaki Nakajima, Eiji Hase, Yasuhiro Mizutani, Hirotsugu Yamamoto, Kaoru Minoshima and Takeshi Yasui : Strain sensing based on strain to radio-frequency conversion of optical frequency comb, Optics Express, Vol.26, No.8, 9484-9491, 2018. (Summary) We propose an optical frequency comb (OFC)-based strain sensing method, namely OFC sensing cavity, which is capable of radio-frequency (RF)-based strain measurement. We developed a null-method-based strain sensing system with a comb-spacing-stabilized OFC generator. We realized strain measurement from 1.83 µ to 1800 µ with a sensing fiber length of 20 mm. The measurable strain frequency range of the developed strain sensing system was from 0 to 310 Hz. Owing to the use of RF-based strain measurement, our approach would be a useful and powerful tool for sensing of strain or other physical quantities, and the concept of the OFC sensing cavity is a new aspect of OFC technology. (Link to Publication Site) ● Publication site (DOI): 10.1364/OE.26.009484 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29715898 ● Summary page in Scopus @ Elsevier: 2-s2.0-85045550276 (DOI: 10.1364/OE.26.009484, PubMed: 29715898, Elsevier: Scopus)
25.	Yuki Ogura, Yuji Tanaka, Eiji Hase, Toyonobu Yamashita and Takeshi Yasui : Texture analysis of secondharmonicgeneration images for quantitative analysis of reticular dermal collagen fibre in vivo in human facial cheek skin, Experimental Dermatology, Vol.28, No.8, 899-905, 2018. (Summary) Secondharmonicgeneration (SHG) microscopy is a powerful tool for in vivo visualisation of collagen fibres in human skin because of its specific collagen selectivity without the need for staining, noninvasiveness and highresolution threedimensional imaging. Although texture analysis of SHG images is a promising method for the quantitative analysis of wellorientated collagen fibre structure in the tendon and cornea, there are few attempts to assess cutaneous ageing. In this study, we applied two texture analysis techniques, namely autocorrelation (2DAC) analysis and twodimensional Fourier transform (2DFT), to evaluate the agedependent changes in reticular dermal collagen fibres in in vivo human cheek skin. Agedependent changes in the reticular dermal collagen fibres of female subjects in their 20s, 40s and 60s clearly appeared in these texture analyses. Furthermore, the parameter from 2DAC analysis showed a significantly higher correlation with skin elasticity measured by a Cutometer®. These results clearly indicate that 2DAC analysis of SHG images is highly promising for the quantitative evaluation of agedependent change in facial collagen fibres as well as skin elasticity. An appropriate texture analysis will help to provide quantitative insight into collagen fibre structure and will be useful for the diagnosis of pathological conditions as well as cutaneous ageing in skin. (Link to Publication Site) ● Publication site (DOI): 10.1111/exd.13560 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29658140 ● Search Scopus @ Elsevier (PMID): 29658140 ● Search Scopus @ Elsevier (DOI): 10.1111/exd.13560 (DOI: 10.1111/exd.13560, PubMed: 29658140)
26.	Takeo Minamikawa, Yi-Da Hsieh, Kyuki Shibuya, Eiji Hase, Yoshiki Kaneoka, Sho Okubo, Hajime Inaba, Yasuhiro Mizutani, Hirotsugu Yamamoto, Tetsuo Iwata and Takeshi Yasui : Dual-comb spectroscopic ellipsometry., Nature Communications, Vol.8, 610, 2017. (Summary) Spectroscopic ellipsometry is a means of investigating optical and dielectric material responses. Conventional spectroscopic ellipsometry is subject to trade-offs between spectral accuracy, resolution, and measurement time. Polarization modulation has afforded poor performance because of its sensitivity to mechanical vibrational noise, thermal instability, and polarization-wavelength dependency. We combine spectroscopic ellipsometry with dual-comb spectroscopy, namely, dual-comb spectroscopic ellipsometry. Dual-comb spectroscopic ellipsometry (DCSE). DCSE directly and simultaneously obtains the ellipsometric parameters of the amplitude ratio and phase difference between s-polarized and p-polarized light signals with ultra-high spectral resolution and no polarization modulation, beyond the conventional limit. Ellipsometric evaluation without polarization modulation also enhances the stability and robustness of the system. In this study, we construct a polarization-modulation-free DCSE system with a spectral resolution of up to 1.2 × 10(-5) nm throughout the spectral range of 1514-1595 nm and achieved an accuracy of 38.4 nm and a precision of 3.3 nm in the measurement of thin-film samples.Spectroscopic ellipsometry is an established technique to characterize the optical properties of a material. Here, Minamikawa et al. combine the method with dual-comb spectroscopy, which allows them to obtain ellipsometric parameters including the phase difference between s-polarized and p-polarized light. (Tokushima University Institutional Repository) ● Metadata: 112462 (Link to Publication Site) ● Publication site (DOI): 10.1038/s41467-017-00709-y (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28931818 ● Search Scopus @ Elsevier (PMID): 28931818 ● Search Scopus @ Elsevier (DOI): 10.1038/s41467-017-00709-y (Tokushima University Institutional Repository: 112462, DOI: 10.1038/s41467-017-00709-y, PubMed: 28931818)
27.	Yuki Ogura, 田中佑治, Eiji Hase, 山下豊信 and Takeshi Yasui : Quantitative Evaluation of Collagen Fiber Structure in Human Dermis Based on Two-Dimensional Auto-Correlation Analysis of SHG (Second Harmonic Generation) Image, Transactions of the Japanese Society for Medical and Biological Engineering, Vol.55, No.2, 97-102, 2017. (Summary) <p>Collagen fibers in the dermis are closely related to skin aging because collagen is one of structural proteins and hence plays an important role to determine the morphology and mechanical properties of the skin. Although histological and/or biochemical studies have been used to reveal the age-related decrease and disorganization of dermal collagen fibers, such invasive methods cannot be used for simple assessment or serial monitoring of skin aging. Therefore, there is a considerable need for non-invasive assessment method of skin aging. Recently, second-harmonic-generation (SHG) microscopy has attracted attention for <i>in situ</i> visualization of human dermal collagen fibers. Due to its specific collagen selectivity without the need for staining, non-invasiveness, and high-resolution 3D imaging capability, collagen fiber structure can be visualized as high-contrast SHG image <i>in situ</i>. However, extraction of quantitative parameters reflecting the collagen fiber structure from SHG image presents difficulties. In this article, we applied two-dimensional auto-correlation analysis to SHG images of dermal collagen fibers in human cheek skin. The collagen fiber structure index obtained from the analysis decreased significantly with aging. Furthermore, we confirmed a moderate correlation between the collagen fiber structure index and skin elasticity measured by a Cutometer<sup>®</sup>.This correlation indicates that the dermal collagen fiber structure plays an important role to determine skin elasticity. In other words, the auto-correlation analysis of SHG image can be used for evaluation of skin elasticity from the viewpoint of dermal collagen. The proposed method will be a powerful tool in the fields of skin cosmetics and anti-aging dermatology.</p> (Keyword) collagen, second-harmonic-generation, skin elasticity, auto-correlation, non-invasive measurement, aging / second-harmonic-generation / skin elasticity / auto-correlation / non-invasive measurement / aging (Link to Publication Site) ● Publication site (DOI): 10.11239/jsmbe.55.97 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001205269205120 ● Search Scopus @ Elsevier (DOI): 10.11239/jsmbe.55.97 (DOI: 10.11239/jsmbe.55.97, CiNii: 1390001205269205120)
28.	Yuki Ogura, Kohsuke Atsuta, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : Hand-held Second-harmonic-generation (SHG) Microscope for Dermatological Application, Transactions of the Japanese Society for Medical and Biological Engineering, Vol.55, No.2, 91-96, 2017. (Summary) Second harmonic generation (SHG) microscopy allows <i>in situ</i> visualization of dermal collagen fibers in human skin due to its selectivity for collagen fiber. It is an attractive tool because it does not require staining, is non-invasiveness, and has high-resolution 3D imaging capability. However, its practical use in the dermatological field is still limited due to the bulky and complicated setup. Incorporation of fiber transmission of the laser light and a compact probe head into the microscope would enhance its compactness, robustness, flexibility, and hence convenience. In this study, we constructed a photonic-crystal-fiber (PCF) -coupled, hand-held SHG microscope for <i>in situ</i> monitoring of collagen fibers in human skin. By transmitting pulse light from a mode-locked Cr:Forsterite laser source via a large mode area PCF, it was possible to deliver pulse light with median wavelength of 1250nm and pulse width of 80 fs to the SHG microscope, without the need for external dispersion compensation. Components of a SHG microscope;galvano mirrors, relay lenses, objective lens, dichroic mirror, optical filters, and photon-counting photomultiplier, were mounted into a hand-held probe head (width=310mm, height=150mm, and depth=50mm), which is only 2% of the size of a conventional SHG microscope. We compared the imaging performance between the conventional SHG microscope and the hand-held SHG microscope, and confirmed comparable performance of the two. Finally, we demonstrated <i>in situ</i> visualization of collagen fibers in human skin using the hand-held SHG microscope with a compact probe head coupled to PCF. The hand-held SHG microscope greatly enhances the operability of <i>in situ</i> measurement of the human skin. The novel system will be a powerful tool for various dermatological applications. (Link to Publication Site) ● Publication site (DOI): 10.11239/jsmbe.55.91 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282680245915008 ● Search Scopus @ Elsevier (DOI): 10.11239/jsmbe.55.91 (DOI: 10.11239/jsmbe.55.91, CiNii: 1390282680245915008)
29.	Eiji Hase, Takeo Minamikawa, Katsuya SATO, Daisuke Yonekura, Mitsuhiko Takahashi and Takeshi Yasui : Observation of Tendon Healing with Second-harmonic-generation (SHG) Microscopy, Transactions of the Japanese Society for Medical and Biological Engineering, Vol.54, No.6, 253-260, 2016. (Summary) <p>Tendon rupture is a traumatic injury that is difficult to recover to the condition before injury. In previous studies, histological staining and tensile testing have been widely used to evaluate histological and mechanical healing. However, since both methods are destructive and invasive, it is difficult to apply these methods to clinical diagnosis. If the degree of healing can be visualized nondestructively and noninvasively, new findings may be obtained regarding mechanisms of the tendon healing process. In this study, we used second-harmonic-generation (SHG) microscopy to evaluate the degree of healing of ruptured tendon in a rabbit model. SHG microscopy has high selectivity and high image contrast with respect to the structural maturity, density, and aggregates of collagen molecule, without the need for histological sectioning and staining. Furthermore, since SHG light intensity sensitively reflects the structural maturity of collagen molecule and its aggregates, it has the potential to be a good indicator for the degree of healing of the injured tendon. By comparing the SHG images between 4-week-healing tendons and normal tendons in the animal model, we confirmed that SHG light intensity of the healing tendon was significantly lower than that of the normal tendon, indicating that the collagen structure in the healing tendon is still immature. Furthermore, we performed image analysis based on 2D Fourier transform of the acquired SHG images, and confirmed a significant difference in collagen distribution depending on the sample. These results indicate that SHG microscopy has the unique potential as an indicator of tendon healing.</p> (Keyword) second-harmonic-generation / microscopy / tendon / collagen / healing / image analysis (Link to Publication Site) ● Publication site (DOI): 10.11239/jsmbe.54.253 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001205270574720 ● Search Scopus @ Elsevier (DOI): 10.11239/jsmbe.54.253 (DOI: 10.11239/jsmbe.54.253, CiNii: 1390001205270574720)
30.	Eiji Hase, Katsuya SATO, Daisuke Yonekura, Takeo Minamikawa, Mitsuhiko Takahashi and Takeshi Yasui : Evaluation of the histological and mechanical features of tendon healing in a rabbit model with the use of second-harmonic-generation imaging and tensile testing, Bone & Joint Research, Vol.5, No.11, 577-585, 2016. (Summary) This study aimed to evaluate the histological and mechanical features of tendon healing in a rabbit model with second-harmonic-generation (SHG) imaging and tensile testing. A total of eight male Japanese white rabbits were used for this study. The flexor digitorum tendons in their right leg were sharply transected, and then were repaired by intratendinous stitching. At four weeks post-operatively, the rabbits were killed and the flexor digitorum tendons in both right and left legs were excised and used as specimens for tendon healing (n = 8) and control (n = 8), respectively. Each specimen was examined by SHG imaging, followed by tensile testing, and the results of the two testing modalities were assessed for correlation. While the SHG light intensity of the healing tendon samples was significantly lower than that of the uninjured tendon samples, 2D Fourier transform SHG images showed a clear difference in collagen fibre structure between the uninjured and the healing samples, and among the healing samples. The mean intensity of the SHG image showed a moderate correlation (R(2) = 0.37) with Young's modulus obtained from the tensile testing. Our results indicate that SHG microscopy may be a potential indicator of tendon healing.Cite this article: E. Hase, K. Sato, D. Yonekura, T. Minamikawa, M. Takahashi, T. Yasui. Evaluation of the histological and mechanical features of tendon healing in a rabbit model with the use of second-harmonic-generation imaging and tensile testing. Bone Joint Res 2016;5:577-585. DOI: 10.1302/2046-3758.511.BJR-2016-0162.R1. (Tokushima University Institutional Repository) ● Metadata: 115027 (Link to Publication Site) ● Publication site (DOI): 10.1302/2046-3758.511.BJR-2016-0162.R1 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27881441 ● Search Scopus @ Elsevier (PMID): 27881441 ● Search Scopus @ Elsevier (DOI): 10.1302/2046-3758.511.BJR-2016-0162.R1 (Tokushima University Institutional Repository: 115027, DOI: 10.1302/2046-3758.511.BJR-2016-0162.R1, PubMed: 27881441)
31.	Shuji Miyamoto, Eiji Hase, Takeo Minamikawa, Yi-Da Hsieh, Yasuhiro Mizurani, Tetsuo Iwata, Takeshi Yasui and Hirotsugu Yamamoto : Scan-Less Full-Field Confocal Microscopy by a Combination of Confocal Slit with Wavelength/Space Conversion, Journal of the Japan Society for Precision Engineering, Vol.82, No.7, 679-682, 2016. (Link to Publication Site) ● Publication site (DOI): 10.2493/jjspe.82.679 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.2493/jjspe.82.679 (DOI: 10.2493/jjspe.82.679)
32.	Eiji Hase, Oki Matsubara, Takeo Minamikawa, Katsuya SATO and Takeshi Yasui : In situ time-series monitoring of collagen fibers produced by standing-cultured osteoblasts using a second-harmonic-generation microscope., Applied Optics, Vol.55, No.12, 3261-3267, 2016. (Summary) In bone tissue engineering and regeneration, there is a considerable need for an unstained method of monitoring collagen fibers produced by osteoblasts. This is because collagen fibers play an important role as a bone matrix and continuous monitoring of their temporal dynamics is important in clarifying the organization process toward forming bone tissue. In the work described here, using a second-harmonic-generation (SHG) microscope, we performed in situ time-series monitoring of collagen fibers produced by cultured osteoblasts without the need for staining. Use of the 19 fs near-infrared pulsed light enables us to visualize the temporal dynamics in a thin layer of collagen fibers produced by a single layer of osteoblasts in high-contrast SHG images. While the collagen fibers were produced and stored inside the osteoblasts at an early stage of culturing, the network structure of collagen fibers was formed and locally condensed at a late stage. Furthermore, we extracted a quantitative parameter of collagen maturity degree in the cultured sample by use of image analysis based on a two-dimensional Fourier transform of the SHG image. The proposed method will be useful for in situ quality and quantity control of collagen fibers in bone tissue engineering and regeneration. (Link to Publication Site) ● Publication site (DOI): 10.1364/AO.55.003261 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27140096 ● Summary page in Scopus @ Elsevier: 2-s2.0-84964507940 (DOI: 10.1364/AO.55.003261, PubMed: 27140096, Elsevier: Scopus)

Academic Paper (Unrefereed Paper):

1.	Takeo Minamikawa, Ogura Takashi, Masuoka Takashi, Eiji Hase, Yoshiaki Nakajima, Yamaoka Yoshihisa, Kaoru Minoshima and Takeshi Yasui : Optical-frequency-comb based ultrasound sensor, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol.10064, 100645C, 2017. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2250792 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85018866599 (DOI: 10.1117/12.2250792, Elsevier: Scopus)
2.	Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Scan-less confocal phase imaging with dual comb microscopy, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol.10076, 100761C, 2017. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2251113 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85020292484 (DOI: 10.1117/12.2251113, Elsevier: Scopus)
3.	Eiji Hase, Takeo Minamikawa, Katsuya SATO, Daisuke Yonekura, Mitsuhiko Takahashi and Takeshi Yasui : Orientation analysis of collagen fibers in healing tendon by using second-harmonic-generation microscopy, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol.10069, 1006909, 2017. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2251111 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85020294503 (DOI: 10.1117/12.2251111, Elsevier: Scopus)
4.	Kosuke Atsuta, Yuki Ogura, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : In situ monitoring of collagen fibers in human skin using a photonic-crystal-fiber-coupled, hand-held, second-harmonic-generation microscope, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol.10069, 100692B, 2017. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2251321 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85020313109 (DOI: 10.1117/12.2251321, Elsevier: Scopus)

Review, Commentary:

1.	Eiji Hase, Takeo Minamikawa, Takahiko Mizuno and Takeshi Yasui : 空間/波長次元変換を用いたワンショット共焦点顕微鏡の高度化∼ポスト光増幅による測定の高速化および蛍光イメージングへの応用∼, 検査技術, Vol.27, No.3, 1-10, Mar. 2022. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1522543656133616768 (CiNii: 1522543656133616768)
2.	Eiji Hase and Takeshi Yasui : SHG顕微鏡の皮膚計測応用, The Review of Laser Engineering, Vol.48, No.12, 665-670, Dec. 2020. (Keyword) Biomedical imaging / Second-harmonic-generation / SHG microscopy / Skin / Collagen (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520853834464616448 (CiNii: 1520853834464616448)
3.	Takeshi Yasui, Eiji Hase, Takahiko Mizuno, Ryo Oe and Takeo Minamikawa : 光周波数コム(光コム)の新奇特徴に基づいた知的光計測, The Journal of the Institute of Electronics, Information and Communication Engineers, Vol.103, No.11, 1126-1131, Nov. 2020. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520290884200033536 (CiNii: 1520290884200033536)
4.	Takuya Tsuda, Takahiko Mizuno, Eiji Hase, Kazuki Nitta, Hidenori Koresawa, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : スキャンレス・デュアル光コム顕微鏡を用いた動体サンプルのイメージング, Optical Alliance, Vol.30, No.6, 11-14, Jun. 2020. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1523106603356335616 (CiNii: 1523106603356335616)
5.	Eiji Hase, Takeo Minamikawa, Takahiko Mizuno, Hirotsugu Yamamoto and Takeshi Yasui : スキャンレスデュアルコム顕微鏡による共焦点位相イメージング, JAPANESE JOURNAL OF OPTICS, Vol.48, No.11, 462-468, Nov. 2019.
6.	Eiji Hase, Takeo Minamikawa, Takahiko Mizuno, Katsuya SATO, Yoshiaki Nakajima, 浅原彰文, Kaoru Minoshima, Yasuhiro Mizurani, Tetsuo Iwata, Hirotsugu Yamamoto and Takeshi Yasui : スキャンレス光コム顕微鏡~光コムを用いたスキャンレス共焦点位相イメージング~, JAPANESE JOURNAL OF OPTICS, Vol.48, No.6, 227, Jun. 2019.
7.	Takeo Minamikawa, Takeshi Yasui, Eiji Hase and Hirotsugu Yamamoto : 空間/波長次元変換を用いたワンショット共焦点顕微鏡, 検査技術, Vol.23, No.11, 6-12, Nov. 2018.
8.	Takeo Minamikawa, Yi-Da Hsieh, Kyuki Shibuya, Eiji Hase, Yoshiki Kaneoka, Sho Okubo, Hajime Inaba, Yasuhiro Mizurani, Hirotsugu Yamamoto, Tetsuo Iwata and Takeshi Yasui : Dual-comb spectroscopic ellipsometry, JAPANESE JOURNAL OF OPTICS, Vol.47, No.6, 242, Jun. 2018.
9.	Takeo Minamikawa, Eiji Hase, Shuji Miyamoto, Hirotsugu Yamamoto and Takeshi Yasui : 波長分散素子を用いたワンショット共焦点顕微鏡, ケミカルエンジニヤリング, Vol.63, No.3, 28-35, Mar. 2018.
10.	Eiji Hase, Shuji Miyamoto, Tatsushi Ichikawa, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : スペクトル・エンコーディングを用いたラインフィールド共焦点デュアル光コム顕微鏡, The Review of Laser Engineering, Vol.45, No.6, 324-327, Jun. 2017.
11.	Takeshi Yasui, Shuji Miyamoto, Eiji Hase, Takeo Minamikawa and Hirotsugu Yamamoto : ライン集光スリットと波長-空間変換を用いたスキャンレス・フルフィールド共焦点レーザー顕微鏡, Optical Alliance, Vol.27, No.11, 10-13, Nov. 2016.
12.	Eiji Hase, Takeo Minamikawa, Takahiko Mizuno, Katsuya SATO, Yoshiaki Nakajima, 浅原彰文, Kaoru Minoshima, Yasuhiro Mizurani, Tetsuo Iwata, Hirotsugu Yamamoto and Takeshi Yasui : 光コムを用いたスキャンレス共焦点位相イメージング, JAPANESE JOURNAL OF OPTICS, Vol.48, No.6, 227, Jun. 2016.

Proceeding of International Conference:

1.	Yudai Matsumura, Eiji Hase, Yu Tokizane, Naoya Kuse, Takeo Minamikawa, Junichi Fujikata, Hiroki Kishikawa, Masanobu Haraguchi, Yasuhiro Okamura, Kaji Takahiro, Otomo Akira, Kanno Atsushi, Hisatake Shintaro and Takeshi Yasui : Dual-wavelength CW lasers injection-locked to optical comb modes for carrier conversion from THz wave to near-infrared light via electro-optical polymer modulator, 48th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz2023), Th-P2-63, Montreal, Sep. 2023.
2.	Takumi Kikuhara, Yoshihiro Makimoto, Yu Tokizane, Naoya Kuse, Eiji Hase, Yudai Matsumura, Hiroki Kishikawa, Atsushi Kanno, Shintaro Hisatake, Yasuhiro Okamura and Takeshi Yasui : Phase moduration in terahertz wave communication using micro-optical comb, 10th International Symposium on Terahertz-Related Devices and Technologies (TeraTech 2023), WeP-23, Sep. 2023.
3.	Yuya Kodama, Hidenori Koresawa, Eiji Hase, Yu Tokizane, Takeo Minamikawa and Takeshi Yasui : Dual-comb spectroscopy of angular surface-plasmon-resonance spectrum using angle-converting optical frequency comb, Photonics and Electromagnetics Research Symposium (PIERS2023), 2A_13, Praha, Jul. 2023.
4.	Yudai Matsumura, Eiji Hase, Yu Tokizane, Naoya Kuse, Junichi Fujikata, Hiroki Kishikawa, Masanobu Haraguchi, Yasuhiro Okamura, Takahiro Kaji, Akira Otomo, Atsushi Kanno, Shintaro Hisatake and Takeshi Yasui : Dual-wavelength, low-phase-noise, optical carrier for terahertz-to-optical carrier conversion with electro-optic polymer modulator, Photonics and Electromagnetics Research Symposium (PIERS2023), 1P4a, Praha, Jul. 2023.
5.	Yudai Matsumura, Eiji Hase, Yu Tokizane, Naoya Kuse, Junichi Fujikata, Hiroki Kishikawa, Masanobu Haraguchi, Yasuhiro Okamura, Takahiro Kaji, Akira Otomo, Atsushi Kanno, Shintaro Hisatake and Takeshi Yasui : Terahertz-to-optical carrier conversion using optical-comb-injection-locked dual-wavelength laser light and electro-optic polymer modulator, Conference on Lasers and Electro-Optics (CLEO) Europe 2023, CC-P11, Munich, Jun. 2023.
6.	Yudai Matsumura, Eiji Hase, Yu Tokizane, Junichi Fujikata, Hiroki Kishikawa, Masanobu Haraguchi, Yasuhiro Okamura, Takahiro Kaji, Akira Otomo, Atsushi Kannno, Shintaro Hisatake, Naoya Kuse and Takeshi Yasui : Carrier conversion from 100-GHz THz wave to dual-wavelength optical carrier injection-locked to optical comb modes using electro-optical polymer modulator for photonic THz detection, Conference on Lasers and Electro-Optics (CLEO) 2023, JTh2A.98, San Jose, May 2023.
7.	Yuya Kodama, Hidenori Koresawa, Eiji Hase, Yu Tokizane, Takeo Minamikawa and Takeshi Yasui : Wavelength-to-angle conversion of optical frequency comb for dual-comb spectroscopy of angular-interrogation surface plasmon resonance, Conference on Lasers and Electro-Optics (CLEO) 2023, STh4K.5, San Jose, May 2023.
8.	Eiji Hase and Takeshi Yasui : Development of second-harmonic-generation microscopy and its application to human skin diagnostics, OPIC2023: LDC2023, LDC6-03, Yokohama, Apr. 2023.
9.	Shinpei Kajiwara, Takeo Minamikawa, Tomoya Okabe, Eiji Hase and Takeshi Yasui : Laser-scanning optical-frequency-comb microscopy for imaging using various aspects of light information, OPIC2023: BISC2023, BISC4-01, Yokohama, Apr. 2023.
10.	Yu Tokizane, Ayato Takashima, Eiji Hase and Takeshi Yasui : Evaluation of measurable axial range in optical vortex interferometer, OPIC2023: ALPS2023, ALPSp1-20, Yokohama, Apr. 2023.
11.	Tomoya Okabe, Keishiroh Ohtani, Shinpei Kaziwara, Eiji Hase, Takeshi Yasui and Takeo Minamikawa : Transmission stage scanning optical frequency comb microscopy for multi-optical-parameter evaluation, pLED International symposium 2023: Exploring Invisible Light Technology, P-29, Tokushima, Mar. 2023.
12.	Yudai Matsumura, Eiji Hase, Yu Tokizane, Naoya Kuse, Junichi Fujikata, Hiroki Kishikawa, Masanobu Haraguchi, Yasuhiro Okamura, Takahiro Kaji, Akira Otomo, Atsushi Kanno, Shintaro Hisatake and Takeshi Yasui : Fundamental study on optical heterodyned terahertz detection using optical-comb-injection-locked dual-wavelength laser light and electro- optic polymer modulator, pLED International symposium 2023: Exploring Invisible Light Technology, P-20, Tokushima, Mar. 2023.
13.	Yuya Kodama, Hidenori Koresawa, Eiji Hase, Yu Tokizane, Takeo Minamikawa and Takeshi Yasui : Observation of angular spectrum of surface plasmon resonance dip using wavelength-to-angle-conversion optical frequency comb, pLED International symposium 2023: Exploring Invisible Light Technology, P-18, Tokushima, Mar. 2023.
14.	Kazuki Yasumaru, Mayuko Shimizu, Eiji Hase, Takeshi Yasui, Koichi Tsuneyama and Takeo Minamikawa : Raman and SHG spectroscopic analyses of lipid droplets accumulated in the liver of a mouse model of nonalcoholic fatty liver disease, pLED International symposium 2023: Exploring Invisible Light Technology, P-11, Tokushima, Mar. 2023.
15.	Tomohiro Tamaki, Takeo Minamikawa, Eiji Hase, Yuki Morimoto, Akihiro Suzuki, Takeshi Yasui, Satoko Nakamura, Akemi Tsutsui, Koichi Takagushi and Koichi Tsuneyama : Second-harmonic generation polarization microscopy to analyze ultra-early-stage liver fibrosis in human non-alcoholic fatty liver disease, pLED International symposium 2023: Exploring Invisible Light Technology, P-5, Tokushima, Mar. 2023.
16.	Ayato Takashima, Yu Tokizane, Eiji Hase and Takeshi Yasui : Optical vortex interferometer using multiple wavelengths, pLED International symposium 2023: Exploring Invisible Light Technology, P-2, Tokushima, Mar. 2023.
17.	Eiji Hase, Takeo Minamikawa, Yu Tokizane and Takeshi Yasui : Analysis of lipid molecular properties in nonalcoholic fatty liver disease by use of Brillouin microspectroscopy, SPIE Biomedical Imaging and Sensing Conference 2022 (BISC2022), 250268, Taipei, Dec. 2022.
18.	Yudai Matsumura, Eiji Hase, Yu Tokizane, Naoya Kuse, Junichi Fujikata, Hiroki Kishikawa, Masanobu Haraguchi, Yasuhiro Okamura, Takahiro Kaji, Akira Otomo, Atsushi Kanno, Shintaro Hisatake and Takeshi Yasui : All-photonic THz detection using CW laser injection-locked to microcomb, 13th International Conference on Organic Nonlinear Optics (ICONO13), P2-13, Nara, Nov. 2022.
19.	Kazuki Sadahiro, Yu Tokizane, Eiji Hase, Takeo Minamikawa, Isao Morohashi and Takeshi Yasui : Wavelength-scanning digital holography based on line-by-line spectrally shaping optical frequency comb, 25th Congress of the International Commission for Optics (ICO-25), PO-33, Dresden, Sep. 2022.
20.	Hidenori Koresawa, Eiji Hase, Yu Tokizane, Takeo Minamikawa and Takeshi Yasui : Measurement of Jones-matrix using dual-comb spectroscopic polarimetry, 25th Congress of the International Commission for Optics (ICO-25), PO-21, Dresden, Sep. 2022.
21.	Kazuki Sadahiro, Yu Tokizane, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : Development of mode-extracting optical frequency comb for rapid wavelength-scanning digital holography, 15th Pacific Rim Conference on Lasers and Electro-Optics 2022 (CLEO-PR2022), P-CTh6-10, Sapporo, Aug. 2022. (Link to Publication Site) ● Publication site (DOI): 10.1364/CLEOPR.2022.P_CTh6_10 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85166471309 (DOI: 10.1364/CLEOPR.2022.P_CTh6_10, Elsevier: Scopus)
22.	Hidenori Koresawa, Eiji Hase, Yu Tokizane, Takeo Minamikawa and Takeshi Yasui : Combination of dual-comb spectroscopy with Jones-matrix polarimetry, 15th Pacific Rim Conference on Lasers and Electro-Optics 2022 (CLEO-PR2022), P-CTh6-09, Sapporo, Aug. 2022. (Link to Publication Site) ● Publication site (DOI): 10.1364/CLEOPR.2022.P_CTh6_09 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85166472034 (DOI: 10.1364/CLEOPR.2022.P_CTh6_09, Elsevier: Scopus)
23.	Kohta Seki, Hidenori Koresawa, Eiji Hase, Yu Tokizane, Takeo Minamikawa, Taka-aki Yano, Taira Kajisa and Takeshi Yasui : Beam-angle-scanning surface plasmon resonance sensor, Optica Imaging and Applied Optics Congress 2022, JTu2A.6, Vancouver, Jul. 2022. (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85139153703 (Elsevier: Scopus)
24.	Eiji Hase and Takeshi Yasui : Orientation analysis of collagen fibers in skin by using polarization-resolved second-harmonic generation microscopy, Proceedings of SPIE, Vol.11934, 11934-21, San Francisco, Feb. 2022.
25.	Hidenori Koresawa, Marc Gouryeb, Kyuki Shibuya, Takahiko Mizuno, Eiji Hase, Yu Tokizane, Ryo Oe, Takeo Minamikawa and Takeshi Yasui : Dual-comb spectroscopic polarimetry for dynamic characterization of polarization property, Proc. SPIE, Vol.11671, 116710H, Online, Mar. 2021. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2583107 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85107199786 (DOI: 10.1117/12.2583107, Elsevier: Scopus)
26.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Scan-less full-field fluorescence lifetime imaging by 2D spectral encoding and dual-comb heterodyne-beating, Conference on Lasers and Electro-Optics (CLEO) 2020, STu4N.5, Online, May 2020. (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85091627420 (Elsevier: Scopus)
27.	Hiroki Takanari, Y Hayashi, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : Quantification of liver fibrosis by SHG microscopy., Focus on Microscopy 2020, Osaka, Apr. 2020.
28.	Eiji Hase, Hiroki Takanari, Mayuko Shimizu, Hayashi Yuri, Takeo Minamikawa, Takeshi Yasui and Koichi Tsuneyama : Characterization of fibrosis in non-alcoholic steatohepatitis by use of second-harmonic-generation microscopy, Asian Pacific Association for the Study of the Liver 2020 (APASL 2020), Bali, Indonesia, Mar. 2020.
29.	Takeo Minamikawa, Mayuko Shimizu, Hiroki Takanari, Shiomi Ryosuke, Kusaka Hiroki, Tanioka Hiroki, Eiji Hase, Takeshi Yasui and Koichi Tsuneyama : Molecular imaging analysis of accumulated fats in non-alcoholic steatohepatitis by Raman microscopy, Asian Pacific Association for the Study of the Liver 2020 (APASL 2020), Bali, Indonesia, Mar. 2020.
30.	Takeo Minamikawa, nakano shota, Eiji Hase, Asahara Akifumi, hidenori koresawa, Takahiko Mizuno, Hirotsugu Yamamoto, Kaoru Minoshima and Takeshi Yasui : Optical-frequency-comb microscopy with laser-scanning configuration for simultaneous and spectroscopic amplitude, quantitative phase, and polarization imaging, Photonics West 2020: OPTO2020, 11287-30, San Francisco, Feb. 2020.
31.	Eiji Hase, Takeshi Yasui, Hirayama Hideki and Kentaro Nagamatsu : The improving resolution for dislocation analysis in GaN by three-photon microscopy, Photonics West 2020: OPTO2020, San Francisco, Feb. 2020.
32.	Takahiko Mizuno, tsuda takuya, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Application of post optical amplification for scan-less confocal amplitude and phase imaging with dual-comb microscopy, Photonics West 2020: BiOS2020, 11250-41, San Francisco, Feb. 2020.
33.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Full-field dual-comb fluorescence lifetime microscopy, Photonics West 2020: BiOS2020, 11250-42, San Francisco, Feb. 2020.
34.	Eiji Hase, Tanaka Ryosuke, Fukushima Shuichiro and Takeshi Yasui : In vivo time-series quantitative evaluation of skin burn healing using second-harmonic-generation microscopy, Photonics West 2020: BiOS2020, 112544-74, San Francisco, Feb. 2020.
35.	Eiji Hase, maeda kosuke, Takahiko Mizuno, Takeo Minamikawa and Takeshi Yasui : Effect of wavelength on polarization-resolved second-harmonic- generation microscopy in thick tissues, Photonics West 2020: BiOS2020, 112544-75, San Francisco, Feb. 2020.
36.	Kenzo Yamaguchi, Eiji Hase, Toshihiro Okamoto, Masanobu Haraguchi and Takeshi Yasui : Gold nanoparticles as enhanced SHG contrast agents for biological imaging, Photonics West 2020: BiOS2020, 11257-39, San Francisco, Feb. 2020.
37.	Tsuda Takuya, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Post-optical-amplification of confocal amplitude and phase images in scan-less confocal dual-comb microscopy, OSA Technical Digest of OSA Laser Congress, JM5A.49, Wien, Sep. 2019. (Link to Publication Site) ● Publication site (DOI): 10.1364/ASSL.2019.JM5A.49 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85086286905 (DOI: 10.1364/ASSL.2019.JM5A.49, Elsevier: Scopus)
38.	Shota Nakano, Takeo Minamikawa, Eiji Hase, Asahara Akifumi, Takahiko Mizuno, Hirotsugu Yamamoto, Kaoru Minoshima and Takeshi Yasui : Laser scanning dual optical-frequency-comb spectromicroscopy, CLEO/Europe-EQEC 2019, CH-10.2CHU, Munich, Jun. 2019.
39.	Takahiko Mizuno, Takuya Tsuda, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Improvement of image quality in dual-comb microscopy by post-amplification of dual comb lights, Conference on Lasers and Electro-Optics (CLEO) 2019, Technical Digest (online), AF3K.4, San Jose, May 2019. (Link to Publication Site) ● Publication site (DOI): 10.1364/CLEO_AT.2019.AF3K.4 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85068144234 (DOI: 10.1364/CLEO_AT.2019.AF3K.4, Elsevier: Scopus)
40.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Scan-less full-field fluorescence microscopy by using 2D spectral disperser and dual-comb optical beats, The 5th Biomedical Imaging and Sensing Conference, BISC-P-09, Yokohama, Apr. 2019. (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85065707665 (Elsevier: Scopus)
41.	Takuya Tsuda, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Implementation of imaging post amplifier on scan-less confocal dual-comb mcroscope, The 5th Biomedical Imaging and Sensing Conference, BISC-P-14, Yokohama, Apr. 2019. (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85065711407 (Elsevier: Scopus)
42.	Takeo Minamikawa, Eiji Hase, Takuya Tsuda, Takahiko Mizuno, Hirotsugu Yamamoto and Takeshi Yasui : Scan-less dual-comb microscopy for confocal amplitude and phase imaging, Focus on Microscopy 2019 (FOM2019), P1-D/10, London, Apr. 2019.
43.	Takuya Sakaue, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : Polarization-resolved SHG microscopy based on continuous rotation of linear polarization with an electro-optic modulator, Proceedings of SPIE, Vol.10882, 10882-103, San Francisco, Feb. 2019.
44.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Scan-less fluorescence imaging using 2D frequency multiplexed illumination by dual-comb optical beat and 2D spectral disperser, Proceedings of SPIE, Vol.10889, 10889-4, San Francisco, Feb. 2019.
45.	Takuya Tsuda, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Scan-less confocal phase imaging of biological samples using dual- comb microscopy, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol.10889, 10889-47, Feb. 2019. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2509244 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85066119791 (DOI: 10.1117/12.2509244, Elsevier: Scopus)
46.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Scan-less fluorescence microscopy by Ccombination of dual-comb optical beats and 2D spectral disperser, KEIO Symposium on Microresonator Frequency Com, P2, Tokyo, Dec. 2018.
47.	Takeo Minamikawa, Shota Nakano, Eiji Hase, Takahiko Mizuno, Hirotsugu Yamamoto and Takeshi Yasui : Fourier transform spectroscopic optical microscopy using dual-comb spectroscopic technique, 13th Pacific Rim Conference on Lasers and Electro-Optics (CLEO-PR 2018), W4F.3, Hong Kong, Aug. 2018. (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85059395964 (Elsevier: Scopus)
48.	Takuya Sakaue, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : Analysis of tissue collagen fibers using rapidly-polarization-modulated second-harmonic-generation microscopy, 13th Pacific Rim Conference on Lasers and Electro-Optics (CLEO-PR 2018), W3A.130, Hong Kong, Aug. 2018.
49.	Eiji Hase, Shuji Miyamoto, Takahiko Mizuno, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Dual-comb microscopy for scanless confocal phase imaging, Conference on Lasers and Electro-Optics (CLEO) 2018, STh3L.3, San Jose, May 2018. (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85052597587 (Elsevier: Scopus)
50.	Eiji Hase, Takeo Minamikawa, Katsuya SATO, Daisuke Yonekura, Mitsuhiko Takahashi and Takeshi Yasui : Quantitative evaluation of healing degree in injured tendons based on orientation analysis of collagen fibers by using Fourier-transform second-harmonic-generation microscopy and its relationship to mechanical property, Proceedings of SPIE, Vol.10711, 10711-26, Yokohama, Apr. 2018. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2318673 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85049237183 (DOI: 10.1117/12.2318673, Elsevier: Scopus)
51.	Eiji Hase, Ryosuke Tanaka, Shu-ichiro Fukushima and Takeshi Yasui : In vivo time-series monitoring of dermal collagen fiber during skin burn healing using second-harmonic-generation microscopy, Proceedings of SPIE, Vol.10711, 10711-42, Yokohama, Apr. 2018. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2316748 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85049235844 (DOI: 10.1117/12.2316748, Elsevier: Scopus)
52.	Yuki Ogura, Kohsuke Atsuta, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : In vivo visualization of dermal collagen fibers in human skin using a photonic-crystal-fiber-coupled, hand-held second-harmonic-generation microscope, Proceedings of SPIE, Vol.10711, 10711-43, Yokohama, Apr. 2018. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2316930 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85049254084 (DOI: 10.1117/12.2316930, Elsevier: Scopus)
53.	Takuya Sakaue, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : Analysis of collagen fiber orientation in biological tissues using polarization-resolved second-harmonic-generation microscopy, Proceedings of SPIE, Vol.10711, 10711-44, Yokohama, Apr. 2018. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2319273 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85049246748 (DOI: 10.1117/12.2319273, Elsevier: Scopus)
54.	Shu-ichiro Fukushima, Makoto Yonetsu, Eiji Hase, Kazuma Takeichi and Takeshi Yasui : In situ monitoring of incised wound healing in animal model using second-harmonic-generation and third-harmonic-generation microscopy, Proceedings of SPIE, Vol.10711, 10711-45, Yokohama, Apr. 2018. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2319458 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85049256106 (DOI: 10.1117/12.2319458, Elsevier: Scopus)
55.	Eiji Hase, Takeo Minamikawa, Shuji Miyamoto, Yasuhiro Mizutani, Tetsuo Iwata, Hirotsugu Yamamoto and Takeshi Yasui : Application of scan-less two-dimensional confocal microscopy achieved by a combination of confocal slit with wavelength/space conversion, Proceedings of SPIE, Vol.10711, 10711-55, Yokohama, Apr. 2018. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2318671 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85049258723 (DOI: 10.1117/12.2318671, Elsevier: Scopus)
56.	Eiji Hase, Takeo Minamikawa, Shuji Miyamoto, Yasuhiro Mizutani, Tetsuo Iwata, Tetsuo Iwata and Takeshi Yasui : Scan-less, line-filed, confocal phase imaging with dual-comb microscopy, Proceedings of SPIE, Vol.10711, 10711-69, Yokohama, Apr. 2018. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2317029 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85049249855 (DOI: 10.1117/12.2317029, Elsevier: Scopus)
57.	Katsuya SATO, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : Quantitative in situ time-series evaluation of osteoblastic collagen synthesis under cyclic strain using second-harmonic-generation microscopy, Proceedings of SPIE, Vol.10711, 107110Z, Yokohama, Apr. 2018. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2316607 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85049249619 (DOI: 10.1117/12.2316607, Elsevier: Scopus)
58.	Shu-ichiro Fukushima, Makoto Yonetsu, Eiji Hase and Takeshi Yasui : Polarization-resolved second-harmonic-generation imaging of dermal collagen fiber in pre-wrinkled skin of ultraviolet-B-exposed mouse, Proceedings of SPIE, Vol.10711, 10711-48, Yokohama, Apr. 2018. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2319360 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85049255656 (DOI: 10.1117/12.2319360, Elsevier: Scopus)
59.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Video-rate confocal phase imaging by use of scan-less dual comb microscopy, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol.10505, 10505-8, San Francisco, Jan. 2018. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2289231 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85047333082 (DOI: 10.1117/12.2289231, Elsevier: Scopus)
60.	Yuki Ogura, Yuji Tanaka, Eiji Hase, Toyonobu Yamashita and Takeshi Yasui : Two-dimensional auto-correlation analysis and Fourier-transform analysis of second-harmonic-generation image for quantitative analysis of collagen fiber in human facial skin, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol.10498, 10498-90, San Francisco, Jan. 2018. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2289226 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85045323228 (DOI: 10.1117/12.2289226, Elsevier: Scopus)
61.	Eiji Hase and Takeshi Yasui : Application of dual comb technique for scan-less confocal phase microscopy, 24th General Congress of Intenational Commission for Optics, Th3A-03, Tokyo, Aug. 2017.
62.	Takuya Sakaue, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : Continuously-polarization-resolved SHG microscopy using electro-optic Pockells cell, 24th General Congress of International Commission for Optics, P5-02, Tokyo, Aug. 2017.
63.	Yuki Ogura, Yuji Tanaka, Eiji Hase, Toyonobu Yamashita and Takeshi Yasui : Comparison of two-dimensional auto-correlation analysis and Fourier transform analysis in secondharmonic-generation image of dermal collagen fibers, 24th General Congress of International Commission on Optics, Tu1C-05, Tokyo, Aug. 2017.
64.	Takeo Minamikawa, Eiji Hase, Shuji Miyamoto, Hirotsugu Yamamoto and Takeshi Yasui : Development of confocal laser scanning microscopy by use of optical frequency comb, Conference on Lasers and Electro-Optics (CLEO) 2017, SF2C.3, San Jose, May 2017. (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85044178075 (Elsevier: Scopus)
65.	Takeo Minamikawa, Eiji Hase, Shuji Miyamoto, Yi-Da Hsieh, Hirotsugu Yamamoto and Takeshi Yasui : Fourier transform-based confocal microscopy using image-encoded optical-frequency-comb, Focus on Microscopy 2017, P2-F/20, Bordeaux, Apr. 2017.
66.	Takeshi Yasui, Eiji Hase, Takeo Minamikawa and Katsuya SATO : In situ second-harmonic-generation imaging of collagen fibers produced by standing-cultured osteoblasts, Focus on Microscopy 2017, P1-E/7, Bordeaux, Apr. 2017.
67.	Eiji Hase, Takeo Minamikawa, Katsuya SATO, Daisuke Yonekura, Mitsuhiko Takahashi and Takeshi Yasui : Orientation analysis of collagen fibers in healing tendon by using second-harmonic-generation microscopy, SPIE Photonics West, Biomedical Optics (BiOS 2017), 10069-8, San Francisco, Feb. 2017. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2251111 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85020294503 (DOI: 10.1117/12.2251111, Elsevier: Scopus)
68.	Takeo Minamikawa, Takashi Ogura, Takashi Masuoka, Eiji Hase, Yoshiaki Nakajima, Yoshihisa Yamaoka, Kaoru Minoshima and Takeshi Yasui : Optical-frequency-comb-based ultrasound sensor, SPIE Photonics West, Biomedical Optics (BiOS 2017), 10064-192, San Francisco, Jan. 2017. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2250792 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85018866599 (DOI: 10.1117/12.2250792, Elsevier: Scopus)
69.	Kosuke Atsuta, Yuki Ogura, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : In situ monitoring of collagen fibers in human skin using a photonic-crystal-fiber-coupled, hand-held, second-harmonic-generation microscope, SPIE Photonics West, Biomedical Optics (BiOS 2017), 10069-81, San Francisco, Jan. 2017. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2251321 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85020313109 (DOI: 10.1117/12.2251321, Elsevier: Scopus)
70.	Shuji Miyamoto, Eiji Hase, Takeo Minamikawa, Takeshi Yasui and Hirotsugu Yamamoto : Video-rate volume imaging confocal microscope based on wavelength / space conversion by use of multichannel spectrometer, Frontier in Optics (FiO) 2016, JTh2A.128, New York, Oct. 2016. (Link to Publication Site) ● Publication site (DOI): 10.1364/FIO.2016.JTh2A.128 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1364/FIO.2016.JTh2A.128 (DOI: 10.1364/FIO.2016.JTh2A.128)
71.	Eiji Hase, Shuji Miyamoto, Yi-Da Hsieh, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : Scan-less, line-field, confocal microscopy based on dimensional-conversion optical frequency comb, Conference on Lasers and Electro-Optics (CLEO) 2016, SW1H.3, San Jose, Jun. 2016. (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85010645273 (Elsevier: Scopus)
72.	Shuji Miyamoto, Eiji Hase, Ryuji Ichikawa, Takeo Minamikawa, Takeshi Yasui and Hirotsugu Yamamoto : One shot confocal microscopy based on wavelength / space conversion by use of multichannel spectrometer, Proceedings of SPIE, Vol.9720, 97201C, San Francisco, Feb. 2016. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2213814 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84982090933 (DOI: 10.1117/12.2213814, Elsevier: Scopus)
73.	Eiji Hase, Takeo Minamikawa, Katsuya SATO, Mitsuhiko Takahashi and Takeshi Yasui : Observation of tendon repair in animal model using second-harmonic-generation microscopy, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol.9712, 97121R, San Francisco, Feb. 2016. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2213890 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84975062041 (DOI: 10.1117/12.2213890, Elsevier: Scopus)
74.	Takeshi Yasui, Eiji Hase, Shuji Miyamoto, Yi-Da Hsieh, Takeo Minamikawa and Hirotsugu Yamamoto : Scan-less, line-field confocal microscopy by combination of wavelength/space conversion with dual optical comb, Proceedings of SPIE, Vol.9720, 972006, San Francisco, Feb. 2016. (Link to Publication Site) ● Publication site (DOI): 10.1117/12.2213560 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84982133275 (DOI: 10.1117/12.2213560, Elsevier: Scopus)

Proceeding of Domestic Conference:

1.	Masayuki Higaki, MIYAMURA Shogo, 田上周路, Yu Tokizane, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : THz コム周波数逓倍による屈折率センシング光コムの高感度化, 第84回応用物理学会秋季学術講演会, 22p-A602-4, Sep. 2023.
2.	HIROKI Kitahama, Hidenori Koresawa, Eiji Hase, 浅原彰文, Takeo Minamikawa and Takeshi Yasui : デュアル光コム分光偏光計による偏光素子の計測, 第84回応用物理学会秋季学術講演会, 21a-A602-4, Sep. 2023.
3.	Naoya Ohkubo, Eiji Hase, KAZUKI Yasumaru, Yu Tokizane, Takeo Minamikawa and Takeshi Yasui : ブリルアン・ラマン散乱顕微鏡を用いた非アルコール性脂肪性疾患における脂質の分子物性解析(2) ~蓄積脂肪滴のブリルアンイメージング~, 第84回応用物理学会秋季学術講演会, 20p-A305-14, Sep. 2023.
4.	Tomohiro Tamaki, Takeo Minamikawa, Eiji Hase, Mayuko Shimizu, Yuki Morimoto, Akihiro Suzuki, Takeshi Yasui, 中村聡子, Akemi Tsutsui, 高口浩一 and Koichi Tsuneyama : ヒト非アルコール性脂肪性肝疾患における超早期肝線維化のSHG偏光顕微鏡解析, 第84回応用物理学会秋季学術講演会, 20p-A305-13, Sep. 2023.
5.	Mayuko Shimizu, Takeo Minamikawa, Eiji Hase, 筒井朱美, Hirohisa Ogawa and Koichi Tsuneyama : A New Approach to Evaluate NASH Pathophysiology Using Liver Tissue, 第112回日本病理学会総会, Apr. 2023. (Keyword) NASH / liver biopsy / SHG / Raman
6.	YUDAI Matsumura, Eiji Hase, Yu Tokizane, Naoya Kuse, Junichi Fujikata, Hiroki Kishikawa, Masanobu Haraguchi, Yasuhiro Okamura, 梶貴博, 大友明, 菅野敦史, 諸橋功, 久武信太郎 and Takeshi Yasui : マイクロ光コム注入同期 CW レーザーを用いたオール光型 THz 検出(2) ~光キャリアと変調サイドバンドのRFビート信号検出~, 第70回応用物理学会春季学術講演会, 17p-A202-4, Mar. 2023.
7.	YUYA Kodama, Hidenori Koresawa, Eiji Hase, Yu Tokizane, Takeo Minamikawa, Tsutomu Araki and Takeshi Yasui : 波長/角度変換光コムを用いた角度SPRスペクトルのデュアル光コム分光, 第70回応用物理学会春季学術講演会, 17p-A502-10, Mar. 2023.
8.	AYATO Takashima, Yu Tokizane, Eiji Hase and Takeshi Yasui : 複数波長を用いた光渦位相計測の検討(2), 第70回応用物理学会春季学術講演会, 17a-A502-4, Mar. 2023.
9.	HIROKI Kitahama, Hidenori Koresawa, Eiji Hase, 浅原彰文, Takeo Minamikawa and Takeshi Yasui : デュアル光コム分光偏光計の安定化に関する検討, 第70回応用物理学会春季学術講演会, 17a-A502-3, Mar. 2023.
10.	Kazuki Sadahiro, Yu Tokizane, Eiji Hase, Takeo Minamikawa, 諸橋功 and Takeshi Yasui : モード抽出光周波数コムを用いたフルカスケードリンク型マルチ合成波⻑デジタル·ホログラフィーによる表面形状測定法, 第70回応用物理学会春季学術講演会, 16p-A303-3, Mar. 2023.
11.	KAZUKI Yasumaru, Takeo Minamikawa, Eiji Hase, 清水真裕子, Takeshi Yasui and Koichi Tsuneyama : 非アルコール性脂肪性肝疾患モデルマウスの肝臓に蓄積した脂肪滴の相転移, 第70回応用物理学会春季学術講演会, 15p-A405-6, Mar. 2023.
12.	Kazuki Furuya, Eiji Hase, Takeo Minamikawa, Takeshi Yasui and Katsuya SATO : Changes in collagen production of osteoblasts subjected to microvibration stimulation including resting phase, 日本機械学会第33回バイオフロンティア講演会講演論文, 2E04, Dec. 2022.
13.	YUYA Kodama, Hidenori Koresawa, Eiji Hase, Yu Tokizane, Takeo Minamikawa and Takeshi Yasui : 角度変換光コムSPRを用いた屈折率センシング, 第7回フォトニクスワークショップ, 1-P-3, Nov. 2022.
14.	HIROKI Kitahama, Hidenori Koresawa, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : デュアル光コム偏光分光測定法の基本特性評価, 第7回フォトニクスワークショップ, 1-P-25, Nov. 2022.
15.	貞廣知輝, Yu Tokizane, Eiji Hase, Takeo Minamikawa, 諸橋功 and Takeshi Yasui : モード抽出電気光学変調器光コムを用いた波長走査・単一波長接続型デジタルホログラフィ, 第7回フォトニクスワークショップ, 1-P-23, Nov. 2022.
16.	YUDAI Matsumura, Eiji Hase, Yu Tokizane, Naoya Kuse, Junichi Fujikata, Hiroki Kishikawa, Masanobu Haraguchi, Yasuhiro Okamura, 梶貴博, 大友明, 菅野敦史, 久武信太郎 and Takeshi Yasui : マイクロ光コム注入同期レーザーを用いた電気光学ポリマー変調デバイスの評価, 第7回フォトニクスワークショップ, 1-P-17, Nov. 2022.
17.	TOMOYA Okabe, Takeo Minamikawa, Keishiroh Ohtani, KAJIWARA Shimpei, Eiji Hase and Takeshi Yasui : 透過型ステージ走査型光コム顕微鏡の開発, 第7回フォトニクスワークショップ, 1-P-21, Nov. 2022.
18.	TOMOHIRO Tamaki, Takeo Minamikawa, Eiji Hase, Mayuko Shimizu, 森本友樹, 鈴木昭浩, Takeshi Yasui, 中村聡子, 筒井朱美, 高口浩一 and Koichi Tsuneyama : SHG 顕微鏡によるヒト非アルコール性脂肪性肝疾患の超早期肝線維化の評価, 第7回フォトニクスワークショップ, 1-P-12, Nov. 2022.
19.	KAZUKI Yasumaru, Takeo Minamikawa, Eiji Hase, Mayuko Shimizu, Takeshi Yasui and Koichi Tsuneyama : 非アルコール性脂肪性肝疾患の肝臓中に蓄積した脂肪滴の分光学的観察, 第7回フォトニクスワークショップ, 1-P-8, Nov. 2022.
20.	TOMOYA Okabe, Takeo Minamikawa, Keishiroh Ohtani, KAJIWARA Shimpei, Eiji Hase and Takeshi Yasui : 透過型光コム顕微鏡の開発と異方性物質の偏光分光イメージング, Optics and Photonics Japan 2022, P24, Nov. 2022.
21.	Hidenori Koresawa, Takeo Minamikawa, Yutaro Onishi, Hayata Tadamasa, Mayuko Shimizu, 森本友樹, Eiji Hase, Takeshi Yasui and Koichi Tsuneyama : ラマン顕微鏡による非アルコール性脂肪性肝疾患における脂肪滴の解析, Optics and Photonics Japan 2022, 16pD6, Nov. 2022.
22.	KAJIWARA Shimpei, Takeo Minamikawa, TOMOYA Okabe, Eiji Hase and Takeshi Yasui : 透過型レーザー走査型光周波数コム顕微鏡によるマルチパラメータ評価, Optics and Photonics Japan 2022, 16Ea11, Nov. 2022.
23.	KAZUKI Yasumaru, Takeo Minamikawa, Eiji Hase, Mayuko Shimizu, Takeshi Yasui and Koichi Tsuneyama : 非アルコール性脂肪性肝疾患モデルマウスの肝臓に蓄積した脂肪滴のラマン・SHGイメージング, Optics and Photonics Japan 2022, 16pD5, Nov. 2022.
24.	HIROKI Kitahama, Hidenori Koresawa, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : 偏光制御パルス列のデュアル光コム分光を用いたジョーンズ行列測定, Optics and Photonics Japan 2022, 15aE9, Nov. 2022.
25.	Kazuki Sadahiro, Yu Tokizane, Eiji Hase, Takeo Minamikawa, 諸橋功 and Takeshi Yasui : モード抽出光コムによる波長走査型・単一波長型デジタルホログラフィーの接続, Optics and Photonics Japan 2022, 14aD3, Nov. 2022.
26.	TOMOHIRO Tamaki, Takeo Minamikawa, Eiji Hase, Mayuko Shimizu, 森本友樹, 鈴木昭浩, Takeshi Yasui, 中村聡子, 筒井朱美, 高口浩一 and Koichi Tsuneyama : SHG顕微鏡によるヒト非アルコール性脂肪性肝疾患の超早期肝線維化の評価, Optics and Photonics Japan 2022, 16pD4, Nov. 2022.
27.	Hidenori Koresawa, Kohta Seki, Eiji Hase, Yu Tokizane, Taira Kajisa, Takeo Minamikawa and Takeshi Yasui : 高速入射角走査型近赤外表面プラズモン共鳴センサーの高度化, 第83回応用物理学会秋季学術講演会, 23p-C302-10, Sep. 2022.
28.	AYATO Takashima, Yu Tokizane, Eiji Hase and Takeshi Yasui : 複数波長を用いた光渦位相計測の検討, 第83回応用物理学会秋季学術講演会, 22p-P01-1, Sep. 2022.
29.	YUYA Kodama, Hidenori Koresawa, Eiji Hase, Takeo Minamikawa, Yu Tokizane, Takeo Minamikawa and Takeshi Yasui : 波長/角度変換光コムを用いた表面プラズモン共鳴法に関する検討, 第83回応用物理学会秋季学術講演会, 22p-C302-12, Sep. 2022.
30.	Kazuki Sadahiro, Yu Tokizane, Eiji Hase, Takeo Minamikawa, 諸橋功 and Takeshi Yasui : 光コムを用いた波長走査型デジタル・ホログラフィーに関する基礎研究(3) ~連続モード抽出10GHz光コムを用いた段差計測~, 第83回応用物理学会秋季学術講演会, 22p-C302-10, Sep. 2022.
31.	Eiji Hase, Takeo Minamikawa, Yu Tokizane and Takeshi Yasui : ブリルアン・ラマン散乱顕微鏡を用いた非アルコール性脂肪性肝疾患における脂質の分子物性解析(1) ~ブリルアン散乱顕微鏡の構築と肝組織のブリルアンイメージング~, 第83回応用物理学会秋季学術講演会, 21p-P03-5, Sep. 2022.
32.	YUDAI Matsumura, Eiji Hase, Yu Tokizane, Naoya Kuse, Junichi Fujikata, Hiroki Kishikawa, Masanobu Haraguchi, Yasuhiro Okamura, 梶貴博, 大友明, 菅野敦史, 久武信太郎 and Takeshi Yasui : マイクロ光コム注入同期CWレーザーを用いたオール光型THz検出(1) ~電気光学ポリマー・デバイスを用いた変調サイドバンドの光スペクトル計測~, 第83回応用物理学会秋季学術講演会, 21p-A202-9, Sep. 2022.
33.	Yutaro Onishi, Takeo Minamikawa, Hidenori Koresawa, Hayata Tadamasa, Mayuko Shimizu, 森本友樹, Eiji Hase, Takeshi Yasui and Koichi Tsuneyama : ラマン顕微鏡を用いた非アルコール性脂肪性肝疾患における脂肪滴サイズと脂質分子物性の関係解析, 第83回応用物理学会秋季学術講演会, 20p-C301-8, Sep. 2022.
34.	Kohta Seki, Hidenori Koresawa, Eiji Hase, Yu Tokizane, Taira Kajisa, Takeo Minamikawa and Takeshi Yasui : 近赤外光を用いた高速入射角走査型表面プラズモン共鳴センサーの開発, 第69回応用物理学会春季学術講演会, 26p-E302-9, Mar. 2022.
35.	Kazuki Sadahiro, Yu Tokizane, Eiji Hase, Takeo Minamikawa, 諸橋功 and Takeshi Yasui : 光コムを用いた波長走査型デジタル・ホログラフィーに関する基礎研究(2) ~モード抽出10GHz光コムの基礎検討~, 第69回応用物理学会春季学術講演会, 26a-E302-7, Mar. 2022.
36.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Yu Tokizane, ryo Oe, Hidenori Koresawa, Hirotsugu Yamamoto and Takeshi Yasui : 全視野デュアル光コム蛍光寿命顕微鏡の開発, 第69回応用物理学会春季学術講演会, 25a-E302-2, Mar. 2022.
37.	Yuki Ogura, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : 偏光分解SHG顕微鏡を用いた目周り皮膚コラーゲンの配向解析, 第69回応用物理学会春季学術講演会, 25p-P08-2, Mar. 2022.
38.	KAJIWARA Shimpei, Takeo Minamikawa, Keishiroh Ohtani, 中野祥太, Eiji Hase and Takeshi Yasui : レーザー走査型光周波数コム顕微鏡による偏光,ToFの定量解析, 第69回応用物理学会春季学術講演会, 25a-E302-62, Mar. 2022.
39.	Hironobu Seki, Kenzo Yamaguchi, Eiji Hase, Takeshi Yasui, Toshihiro Okamoto and Masanobu Haraguchi : 金微粒子を用いた生体コラーゲン第2次高調波増強, 第69回応用物理学会春季学術講演会, 23p-E303-6, Mar. 2022.
40.	Kohta Seki, Hidenori Koresawa, Eiji Hase, Taira Kajisa, Takeo Minamikawa and Takeshi Yasui : 高速入射角走査型表面プラズモン共鳴センサーのバイオセンシング応用, レーザー学会学術講演会第42回年次大会, I03-13a-IX-02, Jan. 2022.
41.	Hidenori Koresawa, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : デュアルコム分光ジョーンズ行列偏光計による偏光光学特性の評価, レーザー学会学術講演会第42回年次大会, E01-12a-V-02, Jan. 2022.
42.	Kohta Seki, Hidenori Koresawa, Eiji Hase, Yu Tokizane, Taira Kajisa, Takeo Minamikawa and Takeshi Yasui : 角度走査型表面プラズモン共鳴センサーの高速化, LED総合フォーラム2022 in 徳島, P-18, Jan. 2022.
43.	Kazuki Sadahiro, Yu Tokizane, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : 高速波長走査型デジタルホログラフィーのためのモード抽出型光コムの開発, LED総合フォーラム2022 in 徳島, P-16, Jan. 2022.
44.	Takeo Minamikawa, Mayuko Shimizu, Hiroki Tanioka, Eiji Hase, Yuki Morimoto, Hiroki Takanari, Takeshi Yasui and Koichi Tsuneyama : ラマン散乱分光法を用いた非アルコール性脂肪性肝疾患の脂質分子構造イメージング解析, レーザ顕微鏡研究会第46回講演会, Nov. 2021.
45.	Kazuki Sadahiro, Yu Tokizane, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : 光コムを用いた高速波長走査型デジタル・ホログラフィーに向けた基礎検討, Optics & Photonics Japan 2021, 28pE5, Oct. 2021.
46.	Kohta Seki, Hidenori Koresawa, Eiji Hase, Taira Kajisa, Takeo Minamikawa and Takeshi Yasui : ガルバノ走査を用いた表面プラズモン共鳴バイオセンサーの高速化, Optics & Photonics Japan 2021, 28pD5, Oct. 2021.
47.	Hiroki Tanioka, Takeo Minamikawa, Mayuko Shimizu, Yuki Morimoto, Hiroki Takanari, Yutaro Onishi, Eiji Hase, Takeshi Yasui and Koichi Tsuneyama : 表面増強ラマン散乱分光法を用いた非アルコール性脂肪肝疾患モデルの高感度ラマンイメージング, 第82回応用物理学会秋季学術講演会予稿集, 13a-N202-9, Sep. 2021.
48.	Seki Kota, (名) koresawa, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : 高速入射角走査型表面プラズモン共鳴センサーの開発, 第82回応用物理学会秋季学術講演会予稿集, 11a-N106-8, Sep. 2021.
49.	Keishiroh Ohtani, Takeo Minamikawa, 中野祥太, Eiji Hase and Takeshi Yasui : 透過型光コム分光顕微鏡の位相安定性の改善, 第82回応用物理学会秋季学術講演会予稿集, 10p-N106-5, Sep. 2021.
50.	Hidenori Koresawa, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : デュアルコム分光ジョーンス行列偏光計を用いた偏光光学素子の計測, 第82回応用物理学会秋季学術講演会予稿集, 10p-N106-10, Sep. 2021.
51.	Kazuki Sadahiro, Yu Tokizane, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : 光コムを用いた波長走査型デジタル・ホログラフィーに関する基礎研究, 第82回応用物理学会秋季学術講演会, 10p-N106-2, Sep. 2021.
52.	Hiroki Tanioka, Takeo Minamikawa, Mayuko Shimizu, Yuki Morimoto, Hiroki Takanari, Ryosuke Shiomi, Eiji Hase, Takeshi Yasui and Koichi Tsuneyama : 非アルコール性脂肪性肝炎診断に対するラマン顕微鏡法の適用可能性の検証計, 第68回応用物理学会春季学術講演会予稿集, 19a-Z04-9, Mar. 2021.
53.	Akihiro Suzuki, masaki ri, Eiji Hase, Katsuya SATO, Kazuhide Mineda, Ichiro Hashimoto and Takeshi Yasui : 偏光分解SHG顕微鏡を用いたケロイド異常産生コラーゲン線維の定量評価, 第68回応用物理学会春季学術講演会予稿集, 19a-Z04-7, Mar. 2021.
54.	Fumitoshi Yagishita, Shota Kunimi, Keita Hoshi, Masaki Okamoto, Atsushi Tabata, Hideaki Nagamune, Eiji Hase, Keiji Minagawa and Yasushi Imada : 多光子励起可能なイミダゾ[1,2-a]ピリジニウム塩の合成と光細胞傷害性, 日本化学会第101春季年会, Mar. 2021.
55.	Keishiroh Ohtani, Takeo Minamikawa, Shota Nakano, Eiji Hase and Takeshi Yasui : 透過型光コム分光顕微鏡の開発, 第68回応用物理学会春季学術講演会予稿集, 16a-Z08-10, Mar. 2021.
56.	Tamaki Yoshikawa, Eiji Hase, 鈴木昭浩, 越智ありさ, Hideharu Abe and Takeshi Yasui : AGE(終末糖化産物)による真皮コラーゲン繊維構造変化のSHG(第二高調波発生光)イメージング, 日本生体医工学会専門別研究会生体画像と医用人工知能研究会第3回研究会若手発表会, Mar. 2021.
57.	Kazuki Furuya, Hirofumi Hosokawa, Eiji Hase, Takeo Minamikawa, Takeshi Yasui and Katsuya SATO : SHG / THG observation of osteoblastic matrix formation process subject to vibration stimuli, 日本機械学会中国四国支部第51回学生員卒業研究発表講演会, Mar. 2021.
58.	Eiji Hase, Yu Tokizane, Takeo Minamikawa and Takeshi Yasui : 光コムシンセサイザーを用いたカスケードリンク型マルチ合成波長デジタルホログラフィ, 第18回医用分光学研究会, Nov. 2020.
59.	Eiji Hase and Takeshi Yasui : 第2高調波発生光顕微鏡を用いたコラーゲン計測, Optics & Photonics Japan 2020, 15aDS2, Nov. 2020.
60.	Akihiro Suzuki, Masaki Ri, Eiji Hase, Katsuya SATO, Kazuhide Mineda, Ichiro Hashimoto and Takeshi Yasui : SHG(第2高調波発生光)顕微鏡を用いたケロイド異常産生コラーゲン線維の観察, Optics & Photonics Japan 2020, 14pA2, Nov. 2020.
61.	Tamaki Yoshikawa, Eiji Hase, Akihiro Suzuki, Arisa Ochi, Hideharu Abe and Takeshi Yasui : AGE(終末糖化産物)による真皮コラーゲン線維構造変化のSHG(第2高調波発生光)イメージング, Optics & Photonics Japan 2020, 14pA3, Nov. 2020.
62.	鈴木昭浩, Masaki Ri, Eiji Hase, Katsuya SATO, Kazuhide Mineda, Ichiro Hashimoto and Takeshi Yasui : ケロイド真皮コラーゲン線維の可視化, 生体医工学シンポジウム2020, 2A-19, Sep. 2020.
63.	Kazuma Kusumoto, Yohei Sanagawa, Eiji Hase, Hiroki Takanari, Atsushi Tabata, Hideaki Nagamune, Yukihiro Arakawa, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : 多光子励起を目指したA-D-A型N-へテロアレーン類のデザインと応用, 2020年web光化学討論会, Sep. 2020.
64.	Ryuta Umebayashi, Masaki Okamoto, Eiji Hase, Hiroki Takanari, Atsushi Tabata, Hideaki Nagamune, Yukihiro Arakawa, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : 多光子励起可能な水溶性イミダゾ[1,2-a]ピリジニウム塩の合成とミトコンドリアイメージング, 2020年web光化学討論会, Sep. 2020.
65.	Fumitoshi Yagishita, Junichi Tanigawa, Masaki Okamoto, Eiji Hase, Hiroki Takanari, Atsushi Tabata, Hideaki Nagamune, Takeo Minamikawa, Takeshi Yasui, Yasushi Imada and Yasuhiko Kawamura : Synthesis of Imidazopyridinium Salts Having Potential Application as Image-guided Photodynamic Therapy Agents, The 100th CSJ Annual Meeting, Mar. 2020.
66.	Takuya Tsuda, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : 次元変換光コムを用いたスキャンレス・フルフィールド共焦点顕微鏡の開発(8) ~共焦点/位相接続による深さダナミックレンジの拡大~, 第67回応用物理学会春季学術講演会予稿集, 14a-B409-9, Mar. 2020.
67.	Kohta Seki, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, 荻博次 and Takeshi Yasui : ピコ秒レーザー超音波を用いたバイオイメージング ∼非同期光サンプリング式ピコ秒レーザー超音波計測∼, 第67回応用物理学会春季学術講演会予稿集, 14p-B409-10, Mar. 2020.
68.	Kosuke Maeda, Eiji Hase, Takahiko Mizuno, Takeo Minamikawa and Takeshi Yasui : In vivoヒト皮膚コラーゲン線維配向計測のための高速連続偏光分解SHG(第2高調波発生光)顕微鏡の開発, LED総合フォーラム2020 in 徳島, P-@, Feb. 2020.
69.	Eiji Hase, Takeo Minamikawa, Shuji Miyamoto, Hirotsugu Yamamoto and Takeshi Yasui : ワンショット・フルフィールド共焦点顕微鏡を用いた植物モニタリング, レーザー学会学術講演会第40回年次大会, X03-22p-XI-03, Jan. 2020.
70.	Eiji Hase and Takeshi Yasui : SHG顕微鏡の皮膚計測応用, レーザー学会学術講演会第40回年次大会, S04-22a-II-06, Jan. 2020.
71.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : デュアル光コムビートによる2次元周波数多重化照明を用いた蛍光寿命イメージング法の開発, Optics & Photonics Japan 2019, 5aB2, Dec. 2019.
72.	Takuya Tsuda, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : 光ファイバー増幅器によるスキャンレス・デュアル光コム顕微鏡の画像増幅, Optics & Photonics Japan 2019, 2aF2, Dec. 2019.
73.	Kosuke Maeda, Eiji Hase, Takahiko Mizuno, yuki ogura, Takeo Minamikawa and Takeshi Yasui : 高速連続偏光分解SHG顕微鏡を用いたヒト真皮コラーゲン線維のin situ深さ分解配向イメージング, Optics & Photonics Japan 2019, 2aF3, Dec. 2019.
74.	Eiji Hase and Takeshi Yasui : デュアル光コム顕微鏡, 第17回医用分光学研究会, Nov. 2019.
75.	Fumitoshi Yagishita, Masaki Okamoto, Hiroki Takanari, Eiji Hase, Atsushi Tabata, Hideaki Nagamune, Yasushi Imada, Takeshi Yasui and Yasuhiko Kawamura : ミトコンドリアイメージングを可能とするイミダゾ[1,2-a]ピリジニウム塩の合成, 2019年日本化学会中国四国支部大会徳島大会, Nov. 2019.
76.	Shota Nakano, Takeo Minamikawa, Eiji Hase, 浅原彰文, Takahiko Mizuno, Katsuya SATO, Hirotsugu Yamamoto, Kaoru Minoshima and Takeshi Yasui : 光コム顕微鏡を用いた統合的光計測プラットホームの開発, ポストLEDフォトニクス公開シンポジウム, Oct. 2019.
77.	Kosuke Maeda, Eiji Hase, Takahiko Mizuno, Takeo Minamikawa and Takeshi Yasui : 高速連続偏光分解SHGイメージングにおける測定深さ依存性の影響, 第80回応用物理学会秋季学術講演会, 21a-E206-9, Sep. 2019.
78.	Shota Nakano, Takeo Minamikawa, Eiji Hase, 浅原彰文, Takahiko Mizuno, Katsuya SATO, Hirotsugu Yamamoto, Kaoru Minoshima and Takeshi Yasui : レーザー走査型光コム分光顕微鏡による生体計測, 第80回応用物理学会秋季学術講演会, 20p-PA4-12, Sep. 2019.
79.	Takuya Tsuda, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : 次元変換光コムを用いたスキャンレス・フルフィールド共焦点顕微鏡の開発(7)~ポスト光増幅イメージの特性評価~, 第80回応用物理学会秋季学術講演会, 20a-E205-9, Sep. 2019.
80.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : デュアル光コム顕微鏡によるスキャンレス蛍光イメージング(4) ∼蛍光寿命イメージング応用∼, 第80回応用物理学会秋季学術講演会, 20a-E205-11, Sep. 2019.
81.	Kosuke Maeda, Eiji Hase, Takahiko Mizuno, Takeo Minamikawa and Takeshi Yasui : 高速連続偏光分解SHG顕微鏡によるin situヒト皮膚コラーゲン線維配向イメージング, 生体医工学シンポジウム2019, 2A-39, Sep. 2019.
82.	Takuya Tsuda, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : スキャンレスデュアル光コム顕微鏡を用いた動体サンプルの共焦点振幅・位相差イメージング, 生体医工学シンポジウム2019, 2A-35, Sep. 2019.
83.	Akira Emoto, Eiji Hase, Kenzo Yamaguchi, 福田隆史 and Takeshi Yasui : コラーゲン線維の明視野下における偏光顕微鏡観察, 生体医工学シンポジウム2019, 2A-19, Sep. 2019.
84.	Shota Nakano, Takeo Minamikawa, Eiji Hase, 浅原彰文, Takahiko Mizuno, Katsuya SATO, Hirotsugu Yamamoto, Kaoru Minoshima and Takeshi Yasui : レーザー走査型光コム定量位相差顕微鏡によるバイオイメージング, 生体医工学シンポジウム2019, 2A-29, Sep. 2019.
85.	Kenzo Yamaguchi, Eiji Hase, Yohhei Kanemura, Hiroki Takanari, Toshihiro Okamoto, Masanobu Haraguchi and Takeshi Yasui : 金ナノ粒子を用いたバイオイメージングにおけるコントラストの増強, 生体医工学シンポジウム2019, 2A-30, Sep. 2019.
86.	Kosuke Maeda, Eiji Hase, Takeo Minamikawa, Takahiko Mizuno, Yuki Ogura and Takeshi Yasui : 高速連続偏光分解SHG顕微鏡を用いたヒト真皮コラーゲン線維配向のin situ可視化, MEとバイオサイバネティックス研究会, Vol.119, No.0913-5685, 43-46, Jul. 2019.
87.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : デュアル光コム顕微鏡によるスキャンレス蛍光イメージング(3) ∼ 蛍光イメージングSNRの改善 ∼, 第66回応用物理学会春季学術講演会, 11p-W935-4, Mar. 2019.
88.	Takuya Tsuda, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : 次元変換光コムを用いたスキャンレス・フルフィールド共焦点顕微鏡の開発(6)~ポスト光増幅によるイメージSN比の向上~, 第66回応用物理学会春季学術講演会, 11p-W935-3, Mar. 2019.
89.	Eiji Hase, Takeo Minamikawa, Takahiko Mizuno, Yuki Ogura and Takeshi Yasui : 高速連続偏光分解SHG顕微鏡を用いたin situ真皮コラーゲン配向解析, 第66回応用物理学会春季学術講演会, 9p-W641-1, Mar. 2019.
90.	Yuto Fukuike, Takeo Minamikawa, Shota Nakano, Ryosuke Shiomi, Eiji Hase, Hirotsugu Yamamoto and Takeshi Yasui : 空間/波長変換を用いたワンショット共焦点顕微鏡による生体組織観察への応用, 第66回応用物理学会春季学術講演会, 9p-W641-2, Mar. 2019.
91.	Shota Nakano, Takeo Minamikawa, Eiji Hase, 浅原彰文, Takahiko Mizuno, Hirotsugu Yamamoto, Kaoru Minoshima and Takeshi Yasui : レーザー走査型光コム分光顕微鏡による偏光計測, 第66回応用物理学会春季学術講演会, 9p-W935-3, Mar. 2019.
92.	Takuya Tsuda, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : スキャンレスデュアル光コム顕微鏡を用いた動体サンプルの共焦点位相差イメージング, Optics & Photonics Japan 2018, 2aA4, Nov. 2018.
93.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : デュアル光コムビートの2 次元周波数多重化ビームを用いた蛍光イメージング法の開発, Optics & Photonics Japan 2018, 2aA5, Nov. 2018.
94.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : デュアル光コム蛍光顕微鏡によるスキャンレス・フルフィールド蛍光イメージング法の開発, 第41回日本生体医工学会中国四国支部大会, Oct. 2018.
95.	Takuya Tsuda, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : 次元変換光コムを用いたスキャンレス・フルフィールド共焦点顕微鏡の開発(5)∼共焦点位相差イメージングの高速化∼, 第79回応用物理学会秋季学術講演会, 19a-438-12, Sep. 2018.
96.	Shota Nakano, Takeo Minamikawa, Eiji Hase, 浅原彰文, Takahiko Mizuno, Hirotsugu Yamamoto, Kaoru Minoshima and Takeshi Yasui : レーザー走査型共焦点光コム分光顕微鏡の開発, 第79回応用物理学会秋季学術講演会, 19p-438-9, Sep. 2018.
97.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : デュアル光コム顕微鏡によるスキャンレス蛍光イメージング(2) ∼520 nm帯光コムによる蛍光イメージング∼, 第79回応用物理学会秋季学術講演会, 19p-438-11, Sep. 2018.
98.	Kazuma Takeichi, Eiji Hase, Takuya Sakaue, 穂積直裕, 吉田祥子 and Takeshi Yasui : SHG顕微鏡，超音波顕微鏡，及びHE染色を用いたコラーゲ線維イメージング, 生体医工学シンポジウム2018, 1A-30, Sep. 2018.
99.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : デュアル光コム顕微鏡によるスキャンレス蛍光イメージング, 第65回応用物理学会春季学術講演会, 19p-C303-14, Mar. 2018.
100.	Takuya Sakaue, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : 偏光分解 SHG(第2高調波発生光) 顕微鏡を用いたコラーゲン線維配向の評価, 第30回バイオエンジニアリング講演会, 2E-10, Dec. 2017.
101.	Takuya Sakaue, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : 電気光学結晶を用いた連続偏光分解第2高調波発生光(SHG)顕微鏡の開発, 第2回フォトニクス研究会「光の極限を探る!」, PTM-21P, Dec. 2017.
102.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : スキャンレスデュアル光コム顕微鏡による共焦点位相差イメージングの高速化, Optics & Photonics Japan 2017, 2aA10, Nov. 2017.
103.	Takuya Sakaue, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : 電気光学的偏光回転を用いた偏光分解第2高調波発生光(SHG)顕微鏡, Optics & Photonics Japan 2017, 2pA6, Nov. 2017.
104.	Takuya Sakaue, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : 偏光分解SHG(第2高調波発生光)顕微鏡を用いたコラーゲン線維配向計測, 第28回バイオフロンティア講演会, 1B11, Oct. 2017.
105.	Kohsuke Atsuta, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : コンパクト第2高調波発生光顕微鏡の開発, 第40回日本生体医工学会中国四国支部大会, IV-6, Oct. 2017.
106.	Kazuma Takeichi, Yuki Ogura, 田中佑治, Eiji Hase, 山下豊信 and Takeshi Yasui : 第2高調波発生光(SHG)顕微鏡における画像解析手法の検討 ~画像自己相関法と画像フーリエ変換法の比較~, 生体医工学シンポジウム2017, 2A-12, Sep. 2017.
107.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : 次元変換光コムを用いたスキャンレス・フルフィールド共焦点顕微鏡の開発(4)~2次元共焦点位相イメージング~, 第78回応用物理学会秋季学術講演会, 6p-A414-11, Sep. 2017.
108.	Shuji Miyamoto, Eiji Hase, Hirotsugu Yamamoto, Takeshi Yasui and Takeo Minamikawa : 波長/空間変換を用いたスキャンレス共焦点レーザー顕微鏡の開発, レーザー学会学術講演会第37回年次大会, Jan. 2017.
109.	Eiji Hase, Takeo Minamikawa, Shuji Miyamoto, Hirotsugu Yamamoto and Takeshi Yasui : スキャンレスデュアル光コム顕微鏡による共焦点位相イメージング, レーザー学会学術講演会第37回年次大会, Jan. 2017.
110.	Shuji Miyamoto, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : 波長分散素子を用いたスキャンレス共焦点レーザー顕微鏡の開発, 第1回フォトニクス研究会, Dec. 2016.
111.	Eiji Hase, Shuji Miyamoto, Hirotsugu Yamamoto, Takeshi Yasui and Takeo Minamikawa : 光コムを用いた共焦点レーザー走査型顕微鏡の開発, Optics&Photonics Japan2016, 2aA8, Nov. 2016.
112.	Eiji Hase, Takeo Minamikawa, Shuji Miyamoto, Hirotsugu Yamamoto and Takeshi Yasui : スキャンレスデュアル光コム顕微鏡を用いた共焦点位相差イメージング, Optics&Photonics Japan2016, 2aA7, Oct. 2016.
113.	Eiji Hase, Takeo Minamikawa, Daisuke Yonekura, Katsuya SATO, Mitsuhiko Takahashi and Takeshi Yasui : SHG顕微鏡を用いた腱修復におけるコラーゲン配向解析, Optics&Photonics Japan2016, 2aA4, Oct. 2016.
114.	Oki Matsubara, Eiji Hase, Takeo Minamikawa, Takeshi Yasui and Katsuya SATO : in vivo analysis of collagen fiber orientation produced by cyclically stretched osteoblast like cells with polarization-solved SHG microscopy, 第27回バイオフロンティア講演会講演論文集, 101-102, Oct. 2016.
115.	Takuya Sakaue, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : 偏光分解第2高調波発生光顕微鏡を用いた組織コラーゲン配向の計測, 第39回日本生体医工学会中国四国支部大会講演抄録, II-1, Oct. 2016.
116.	Eiji Hase, Takeo Minamikawa, Katsuya SATO, Daisuke Yonekura, Mitsuhiko Takahashi and Takeshi Yasui : 偏光分解SHG(第2高調波発生光)顕微鏡を用いた修復腱におけるコラーゲン配向解析, 第39回日本生体医工学会中国四国支部大会講演抄録, I-7, Oct. 2016.
117.	Shuji Miyamoto, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : 波長/空間変換およびマルチチャネル分光器を用いたワンショット・フルフィールド共焦点光学顕微鏡の開発, 第39回日本生体医工学会中国四国支部大会講演抄録, II-1, Oct. 2016.
118.	Oki Matsubara, Eiji Hase, Takeo Minamikawa, Takeshi Yasui and Katsuya SATO : 偏光分解SHG顕微鏡を用いた繰り返し伸縮刺激を受ける骨芽細胞産生コラーゲンのin vivo線維配向解析, 第39回日本生体医工学会中国四国支部大会講演抄録, 17, Oct. 2016.
119.	Yuki Ogura, 田中佑治, Eiji Hase, 山下豊信 and Takeshi Yasui : 画像解析手法を用いた第二高調波発生によるコラーゲンin vivoイメージングの定量化と，肌弾力性との関連, 生体医工学シンポジウム2016, 2P-5-7, Sep. 2016.
120.	Yuki Ogura, Kohsuke Atsuta, Eiji Hase, Takeo Minamikawa and Takeshi Yasui : 第2高調発生光顕微鏡の小型化, 生体医工学シンポジウム2016, 2P-5-8, Sep. 2016.
121.	Eiji Hase, Takeo Minamikawa, Katsuya SATO, Daisuke Yonekura, Mitsuhiko Takahashi and Takeshi Yasui : SHG顕微鏡を用いた腱修復の観測, 生体医工学シンポジウム2016, 2P-5-6, Sep. 2016.
122.	Eiji Hase, Shuji Miyamoto, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : 次元変換光コムを用いたスキャンレス・フルフィールド共焦点顕微鏡の開発(3)~2次元共焦点イメージの取得~, 第77回応用物理学会秋季学術講演会予稿集, 15a-C42-6, Sep. 2016.
123.	Eiji Hase, Shuji Miyamoto, Hirotsugu Yamamoto, Takeshi Yasui and Takeo Minamikawa : 共焦点レーザー走査型光コムの開発, 第77回応用物理学会秋季学術講演会予稿集, 15p-C32-8, Sep. 2016.
124.	Takeo Minamikawa, Eiji Hase, Shuji Miyamoto, Yi-Da Hsieh, Yasuhiro Mizurani, Hirotsugu Yamamoto and Takeshi Yasui : 光コムの波長/空間次元変換を援用したスキャンレス共焦点光学顕微鏡, 2016年度精密工学会秋季大会学術講演会, B18, Sep. 2016.
125.	Kohsuke Atsuta, Eiji Hase, Takeo Minamikawa, 吉木啓介 and Takeshi Yasui : SHG(第二高調波発生)顕微鏡の小型化, 第63回応用物理学会春季学術講演会予稿集, 21p-S422-7, Mar. 2016.
126.	Eiji Hase, Shuji Miyamoto, Yi-Da Hsieh, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : 次元変換光コムを用いたスキャンレス・フルフィールド共焦点顕微鏡の開発(2)~2次元波長分散光学系の構築~, 第63回応用物理学会春季学術講演会予稿集, 21a-H116-11, Mar. 2016.
127.	Shuji Miyamoto, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : 波長/1D空間変換および共焦点スリットを用いたフルフィールド・スキャンレス共焦点レーザー顕微鏡の開発, 第63回応用物理学会春季学術講演会予稿集, 20p-H116-9, Mar. 2016.
128.	Takeshi Yasui, Shuji Miyamoto, Eiji Hase, Takeo Minamikawa, Yasuhiro Mizurani and Hirotsugu Yamamoto : 共焦点スリットと波長/空間変換を用いたスキャンレス・フルフィールド共焦点レーザー顕微鏡の開発, 2016年度精密工学会春季大会学術講演会, T66, Mar. 2016.

Et cetera, Workshop:

1.	YUDAI Matsumura, Eiji Hase, Yu Tokizane, Naoya Kuse, Junichi Fujikata, Hiroki Kishikawa, Masanobu Haraguchi, Yasuhiro Okamura, 梶貴博, 大友明, 菅野敦史, 久武信太郎 and Takeshi Yasui : 電気光学ポリマー変調器と光コムモードに注入同期された 2波長CWレーザーを用いたTHz波から近赤外光へのキャリア変換, 第7回超高速光エレクトロニクス研究会 "超高速ダイナミクスを探る先端光源と計測技術", Apr. 2023.
2.	Naoya Ohkubo, Eiji Hase and Takeshi Yasui : 皮膚AGE評価のための蛍光寿命顕微鏡の開発, 第7回超高速光エレクトロニクス研究会 "超高速ダイナミクスを探る先端光源と計測技術", Apr. 2023.
3.	AYATO Takashima, Yu Tokizane, Eiji Hase and Takeshi Yasui : 複数波長を用いた光渦位相計測の検討, 次世代光フォーラム2023 in 徳島, P-17, Feb. 2023.
4.	YUDAI Matsumura, Eiji Hase, Yu Tokizane, Naoya Kuse, Junichi Fujikata, Hiroki Kishikawa, Masanobu Haraguchi, Yasuhiro Okamura, 梶貴博, 大友明, 菅野敦史, 久武信太郎 and Takeshi Yasui : 光コム注入同期2波長レーザー光と電気光学ポリマー変調器を用いた光ヘテロダイン式テラヘルツ検出に関する基礎検討, 次世代光フォーラム2023 in 徳島, P-15, Feb. 2023.
5.	TOMOYA Okabe, Takeo Minamikawa, 大谷圭史朗, KAJIWARA Shimpei, Eiji Hase and Takeshi Yasui : 光コムを用いた透過型ステージ走査型顕微鏡による複数の光学パラメーターの同時評価, 次世代光フォーラム2023 in 徳島, P-13, Feb. 2023.
6.	TOMOHIRO Tamaki, Takeo Minamikawa, Eiji Hase, Mayuko Shimizu, Yuki Morimoto, Akihiro Suzuki, Takeshi Yasui, 中村聡子, Akemi Tsutsui, 高口浩一 and Koichi Tsuneyama : ヒト非アルコール性脂肪性肝疾患における超早期肝線維化のSHG 偏光顕微鏡解析, 次世代光フォーラム2023 in 徳島, P-12, Feb. 2023.
7.	KAZUKI Yasumaru, Takeo Minamikawa, Eiji Hase, 清水真裕子, Takeshi Yasui and Koichi Tsuneyama : 非アルコール性脂肪性肝疾患モデルマウスに蓄積した脂肪滴の物性解明, 次世代光フォーラム2023 in 徳島, P-11, Feb. 2023.
8.	YUYA Kodama, Hidenori Koresawa, Eiji Hase, Yu Tokizane, Takeo Minamikawa and Takeshi Yasui : 波長/角度変換光コムSPR(表面プラズモン共鳴法)における入射角度範囲の拡大, 次世代光フォーラム2023 in 徳島, P-7, Feb. 2023.
9.	Hiroki Tanioka, Takeo Minamikawa, Mayuko Shimizu, 森本友樹, Hiroki Takanari, Ryosuke Shiomi, Eiji Hase, Takeshi Yasui and Koichi Tsuneyama : 非アルコール性脂肪肝炎の蓄積した脂肪に着目したラマン分光イメージング, LED総合フォーラム2021 in 徳島, P-14, Feb. 2021.
10.	Keishiroh Ohtani, Takeo Minamikawa, 中野祥太, Eiji Hase and Takeshi Yasui : レーザー走査型光コム分顕微鏡による生体イメージング, LED総合フォーラム2021 in 徳島, P-15, Feb. 2021.
11.	Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : デュアル光コム分光法におけるロックイン検出の利用, LED総合フォーラム2019 in 徳島, P-19, Feb. 2019.
12.	Takuya Tsuda, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto and Takeshi Yasui : フルフィールド共焦点顕微鏡による共焦点位相差イメージング, LED総合フォーラム2019 in 徳島, P-21, Feb. 2019.
13.	Shota Nakano, Takeo Minamikawa, Eiji Hase, 浅原彰文, Takahiko Mizuno, Hirotsugu Yamamoto and Takeshi Yasui : レーザー走査型光コム顕微鏡の開発, LED総合フォーラム2019 in 徳島, P-23, Feb. 2019.
14.	Yuto Fukuike, Takeo Minamikawa, Shota Nakano, Ryosuke Shiomi, Eiji Hase and Takeshi Yasui : 空間/波長変換を用いたワンショット共焦点顕微鏡の開発と角膜組織診断への応用, LED総合フォーラム2019 in 徳島, P-24, Feb. 2019.

Patent:

1.	Takeshi Yasui, Naoya Kuse, Yu Tokizane, Eiji Hase, Hiroki Kishikawa, Yasuhiro Okamura and Junichi Fujikata : コヒーレント合成光電気変換装置, PCT/JP2023/ 26378 (Jul. 2023), .
2.	Takeshi Yasui, Naoya Kuse, Yu Tokizane, Eiji Hase, Hiroki Kishikawa, Yasuhiro Okamura, 梶貴博, 鎌田隼, 諸橋功 and 久武信太郎 : 無線受信装置, 2022-134378 (Aug. 2022), .
3.	Takeshi Yasui, Naoya Kuse, Yu Tokizane, Eiji Hase, Hiroki Kishikawa, Yasuhiro Okamura and Junichi Fujikata : コヒーレント合成光電気変換装置, 2022-115259 (Jul. 2022), .
4.	Takeshi Yasui, Naoya Kuse, Yu Tokizane, Eiji Hase, Hiroki Kishikawa, Yasuhiro Okamura and Junichi Fujikata : 周波数多重無線伝送装置, 2022-82057 (May 2022), .
5.	Takeshi Yasui, Naoya Kuse, Yu Tokizane, Eiji Hase, Hiroki Kishikawa, Yasuhiro Okamura and Junichi Fujikata : 光電気変換装置, 2022-82054 (May 2022), .
6.	Takeshi Yasui, Tetsuo Iwata, Yasuhiro Mizurani, Takeo Minamikawa, Eiji Hase and Hirotsugu Yamamoto : 計測装置及び照射装置, PCT/JP2018/029924 (Aug. 2018), WO2019/031584 (Feb. 2019), US10837906 (Nov. 2021).
7.	Takeo Minamikawa, Eiji Hase, Shuji Miyamoto and Takeshi Yasui : 共焦点顕微鏡及び画像化システム, 2017-188816 (Sep. 2017), 2021-028644 (Feb. 2021), .
8.	Takeshi Yasui, Tetsuo Iwata, Yasuhiro Mizurani, Takeo Minamikawa, Eiji Hase and Hirotsugu Yamamoto : 計測装置及び照射装置, 2017-154392 (Aug. 2017), .
9.	Takeshi Yasui, Tetsuo Iwata, Yasuhiro Mizurani, Takeo Minamikawa, Yi-Da Hsieh, Eiji Hase and Hirotsugu Yamamoto : 計測装置, PCT/JP2016/66636 (Jun. 2016), WO2017/002535 (Jan. 2017), .
10.	Takeshi Yasui, Tetsuo Iwata, Yasuhiro Mizutani, Yi-Da Hsieh, Eiji Hase and Hirotsugu Yamamoto : 計測装置, 2015126427 (Jun. 2015), .

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

非線形光散乱を駆使した線維化における細胞機能解析 (Project/Area Number: 21K18038 )
Micromechanical analysis of cortical bone in type 2 diabetes by using synchrotron radiation X-ray diffraction and scattering (Project/Area Number: 19K20691 )
Dual-comb microscopy~Will optical frequency combs bring innovative advances to confocal microscopy?~ (Project/Area Number: 19H00871 )
4D imaging of bio-soft material deformation based on phase-contrast CT using synchrotron light (Project/Area Number: 18K19926 )
One-shot dual-comb 3D microscopy and spectroscopic imaging to evaluate moisture content of a living plant (Project/Area Number: 15H02026 )
Search by Researcher Number (50805512)

Designated Assistant Professor : Hase, Eiji

Research

Personal Web Page

Field of Study

Subject of Study

Book / Paper

Book:

Academic Paper (Judged Full Paper):

Academic Paper (Unrefereed Paper):

Review, Commentary:

Proceeding of International Conference:

Proceeding of Domestic Conference:

Et cetera, Workshop:

Patent:

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)