Tokushima University / Educator and Researcher Directory --- Hosokawa, Yuki

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Tokushima University ⟩ Graduate School of Biomedical Sciences ⟩ 歯学域 ⟩ Oral Science ⟩ Clinical Dentistry ⟩ Department of Conservative Dentistry ⟩
Tokushima University ⟩ University Hospital ⟩ Clinical Division ⟩ Department of General Dentistry ⟩ Conservative Dentistry ⟩
Tokushima University ⟩ Faculty of Dentistry ⟩ Course in Dentistry ⟩ Clinical Dentistry for Oral Health and Function ⟩ Conservative Dentistry ⟩

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Research

Field of Study

Subject of Study

Book / Paper

Academic Paper (Judged Full Paper):

1.	Akihiro Wada, Keiji Murakami, Yumi Ishikawa, Takashi Amoh, Kouji Hirao, Yuki Hosokawa, Daisuke Hinode, Yoichiro Miyake and Hiromichi Yumoto : Anti-Inflammatory and Protective Effects of Juncus effusus L. Water Extract on Oral Keratinocytes, BioMed Research International, Vol.2022, 9770899, 2022. (Summary) Periodontitis is a chronic inflammatory disease caused by periodontopathogenic bacteria that form biofilms in periodontal pockets. The gingival epithelium acts as the first physical barrier in fighting attacks by periodontopathogenic pathogens, such as the primary etiological agent , and various exogenous chemicals, as well as regulates the local innate immune responses. Therefore, the development of novel oral care products to inhibit inflammatory reactions caused by bacterial infection and protect the gingival epithelium is necessary. L. has generally been used as an indigenous medicine, such as a diuretic, an antipyretic, and an analgesic, in ancient practice. In this study, we examined the effects of a water extract from L. on the inhibition of the inflammatory reaction elicited by bacterial infection and protection of the oral epithelium by chemical irritation. Pretreatment of oral epithelial cells with the water extract from L. significantly reduced or its lipopolysaccharide- (LPS-) mediated production of chemokines (interleukin-8 and C-C-chemokine ligand20) in a concentration-dependent manner with comparable to or greater effects than epigallocatechin gallate and protected oral epithelial cells from injury by chemical irritants, cetylpyridinium chloride, and benzethonium chloride. Moreover, the water extract from . L. in the presence of antimicrobial agents or antifibrinolytics already used as ingredients in mouthwash could significantly reduce the production of chemokines from LPS-stimulated oral epithelial cells in a concentration-dependent manner. These findings suggest that the water extract from . L. is potentially useful for oral care to prevent oral infections, such as periodontal infections, and maintain oral epithelial function. (Keyword) Anti-Inflammatory Agents / Bacteroidaceae Infections / Cell Line, Transformed / Humans / Keratinocytes / Magnoliopsida / Mouth Mucosa / Periodontitis / Plant Extracts / Porphyromonas gingivalis (Tokushima University Institutional Repository) ● Metadata: 117031 (Link to Publication Site) ● Publication site (DOI): 10.1155/2022/9770899 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35028318 ● Summary page in Scopus @ Elsevier: 2-s2.0-85123567270 (Tokushima University Institutional Repository: 117031, DOI: 10.1155/2022/9770899, PubMed: 35028318, Elsevier: Scopus)
2.	Hitomi Kuramoto, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Tadashi Nakanishi, Daisuke Takegawa, Ayako Washio, Chiaki Kitamura and Takashi Matsuo : Caffeic acid phenethyl ester (CAPE) induces VEGF expression and production in rat odontoblastic cells, BioMed Research International, Vol.Article ID 5390720, 2019. (Summary) Caffeic acid phenethyl ester (CAPE), the main component of propolis, has various biological activities including anti-inflammatory effect and wound healing promotion. Odontoblasts located in the outermost layer of dental pulp play crucial roles such as production of growth factors and formation of hard tissue termed reparative dentin in host defense against dental caries. In this study, we investigated the effects of CAPE on the upregulation of vascular endothelial growth factor (VEGF) and calcification activities of odontoblasts, leading to development of novel therapy for dental pulp inflammation caused by dental caries. CAPE significantly induced mRNA expression and production of VEGF in rat clonal odontoblast-like KN-3 cells cultured in normal medium or osteogenic induction medium. CAPE treatment enhanced nuclear factor-kappa B (NF-B) transcription factor activation, and furthermore, the specific inhibitor of NF-B significantly reduced VEGF production. The expression of VEGF receptor- (VEGFR-) 2, not VEGFR-1, was up regulated in KN-3 cells treated with CAPE. In addition, VEGF significantly increased mineralization activity in KN-3 cells. These findings suggest that CAPE might be useful as a novel biological material for the dental pulp conservative therapy. (Tokushima University Institutional Repository) ● Metadata: 114566 (Link to Publication Site) ● Publication site (DOI): 10.1155/2019/5390720 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31930126 ● Summary page in Scopus @ Elsevier: 2-s2.0-85077441042 (Tokushima University Institutional Repository: 114566, DOI: 10.1155/2019/5390720, PubMed: 31930126, Elsevier: Scopus)
3.	Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Hitomi Kuramoto, 鷲尾絢子, Tadashi Nakanishi, Daisuke Takegawa, 北村知昭 and Takashi Matsuo : Anti-inflammatory Effects of Catechin on Rat Odontoblastic Cells (KN-3), The Japanese Journal of Conservative Dentistry, Vol.60, No.5, 235-244, 2017. (Link to Publication Site) ● Publication site (DOI): 10.11471/shikahozon.60.235 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282680498132224 ● Search Scopus @ Elsevier (DOI): 10.11471/shikahozon.60.235 (DOI: 10.11471/shikahozon.60.235, CiNii: 1390282680498132224)

Review, Commentary:

1.	Daisuke Takegawa, Kazuhide Yonekura, Hitomi Kuramoto, Yumika Ida, Yuki Hosokawa, Ikuko Hosokawa, Yoshitaka Hosokawa, Toshiyuki Suge, Tadashi Nakanishi and Keiichi Hosaka : Clinical Key Points of Class II Direct Composite Restoration, Journal of Oral Health and Biosciences, Vol.36, No.1, 8-12, Sep. 2023. (Keyword) dental care (Tokushima University Institutional Repository) ● Metadata: 118637 (Link to Publication Site) ● Publication site (DOI): 10.20738/johb.36.1_8 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390016062665364224 ● Search Scopus @ Elsevier (DOI): 10.20738/johb.36.1_8 (Tokushima University Institutional Repository: 118637, DOI: 10.20738/johb.36.1_8, CiNii: 1390016062665364224)
2.	Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa, Hitomi Kuramoto, Daisuke Takegawa, Tadashi Nakanishi and Takashi Matsuo : The Roles of Odontoblasts in Dental Pulp Innate Immunity, Japanese Dental Science Review, Vol.54, No.3, 105-117, Aug. 2018. (Summary) Odontoblasts located in the outermost layer of dental pulp form a natural barrier between mineralized tissues, dentin, and soft tissues, dental pulp, of the vital tooth, and they first recognize caries-related pathogens and sense external irritations. Therefore, odontoblasts possess a specialized innate immune system to fight oral pathogens invading into dentin. Generally, the rapid initial sensing of microbial pathogens, especially pathogen-associated molecular patterns (PAMPs) shared by microorganisms, are mediated by pattern recognition receptors (PRRs), such as Toll-like receptor and the nucleotide-binding oligomerization domain (NOD). The innate immune responses in odontoblasts initiated by sensing oral pathogens provide host protective events, such as inflammatory reactions, to produce a variety of pro-inflammatory mediators, including chemokines and cytokines. These attract various inflammatory cells and cause antibacterial reactions, such as the production of defensins, to kill microorganisms in the proximal region of the odontoblast layer. This review focuses on innate immunity, especially cellular and molecular mechanisms regarding the sensing of PAMPs from oral pathogens by PRRs, in odontoblasts and provides information for future studies for the development of novel therapeutic strategies, including diagnosis and treatment, to prevent exceeding dental pulp inflammation and preserve the dental pulp tissues. (Tokushima University Institutional Repository) ● Metadata: 112960 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jdsr.2018.03.001 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30128058 ● Summary page in Scopus @ Elsevier: 2-s2.0-85044379499 (Tokushima University Institutional Repository: 112960, DOI: 10.1016/j.jdsr.2018.03.001, PubMed: 30128058, Elsevier: Scopus)

Proceeding of International Conference:

1.	Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa, Hitomi Kuramoto, Katsuhiko Hirota, Yoichiro Miyake and Takashi Matsuo : Streptococcal histone-like DNA binding protein induces MINCLE expression on monocytes, 95th General Session & Exhibition of the International Association for Dental Research, San Francisco, Mar. 2017.

Proceeding of Domestic Conference:

1.	Hitomi Kuramoto, Tadashi Nakanishi, Daisuke Takegawa, Yuki Hosokawa, Katsuhiro Mieda and Keiichi Hosaka : The effects of Caffeic acid phenethyl ester (CAPE) on VEGF production in dental pulp cells, 日本歯科保存学会2021年度秋季学術大会(第155回), Oct. 2021.
2.	Hitomi Kuramoto, Kouji Hirao, Yuki Hosokawa, Daisuke Takegawa, Hiromichi Yumoto and Tadashi Nakanishi : ラット象牙芽細胞(KN-3)におけるVEGFの石灰化誘導作用, 日本歯科保存学会 2019年度秋季学術大会(第151回) (福岡), Nov. 2019.
3.	Daisuke Takegawa, Tadashi Nakanishi, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Hitomi Kuramoto and Takashi Matsuo : Effects of interferon-γ on innate host responses in cultured odontoblast-like cells stimulated with NOD ligands, 第150回日本歯科保存学会 2019年度春季学術大会, Jun. 2019.
4.	Hitomi Kuramoto, Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa and Takashi Matsuo : Caffeic Acid Phenetyl Ester (CAPE)のラット象牙芽細胞(KN-3)におけるVEGF産生誘導機序の解析, 第40回日本歯内療法学会学術大会 (東京), Jun. 2019.
5.	Hitomi Kuramoto, Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa, Tadashi Nakanishi, Daisuke Takegawa and Takashi Matsuo : Caffeic Acid Phenetyl Ester (CAPE)の象牙芽細胞と骨芽細胞におけるVEGF産生誘導機序の解析, 第148回日本歯科保存学会春季学術大会, Jun. 2018.
6.	Kouji Hirao, Hitomi Kuramoto, Hiromichi Yumoto, Yuki Hosokawa and Takashi Matsuo : プロポリス生理活性物質(CAPE; Caffeic Acid Phenetyl Ester)を用いた新規覆髄材の開発, 第17回西日本歯内療法学会研修会, Sep. 2017.
7.	Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Hitomi Kuramoto and Takashi Matsuo : ヒト歯髄組織ならびにラット象牙芽細胞(KN-3)におけるMincle発現と象牙芽細胞での機能解析, 第38回日本歯内療法学会学術大会 & 第14回日韓合同歯内療法学会学術大会, Jul. 2017.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

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