徳島大学 / 教育研究者総覧 --- 西川泰史

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研究活動

専門分野

○	歯周病

研究テーマ

○	歯周病学

著書・論文

学術論文(審査論文):

1.	Yasufumi Nishikawa, Yukari Kajiura, Hwan Jung Lew, Jun-ichi Kido, Toshihiko Nagata and Koji Naruishi : Calprotectin Induces IL-6 and MCP-1 Production via Toll-Like Receptor 4 Signaling in Human Gingival Fibroblasts., Journal of Cellular Physiology, Vol.232, No.7, 1862-1871, 2017. (要約) Calprotectin, a heterodimer of S100A8 and S100A9 molecules, is associated with inflammatory diseases such as inflammatory bowel disease. We have reported that calprotectin levels in gingival crevicular fluids of periodontitis patients are significantly higher than in healthy subjects. However, the functions of calprotectin in pathophysiology of periodontitis are still unknown. The aim of this study is to investigate the effects of calprotectin on the productivity of inflammatory cytokines in human gingival fibroblasts (HGFs). The HGFs cell line CRL-2014® (ATCC) were cultured, and total RNAs were collected to examine the expression of TLR2/4 and RAGE mRNA using RT-PCR. After the cells were treated with S100A8, S100A9, and calprotectin, supernatants were collected and the levels of IL-6 and MCP-1 were measured using ELISA methods. To examine the intracellular signals involved in calprotectin-induced cytokine production, several chemical inhibitors were used. Furthermore, after the siRNA-mediated TLR4 down-regulated cells were treated with S100A8, S100A9, and calprotectin, the levels of IL-6 and MCP-1 were also measured. HGFs showed greater expression of TLR4 mRNA, but not TLR2 and RAGE mRNA compared with human oral epithelial cells. Calprotectin increased significantly the production of MCP-1 and IL-6 in HGFs, and the cytokine productions were significantly suppressed in the cells treated with MAPKs, NF-B, and TLR4 inhibitors. Furthermore, calprotectin-mediated MCP-1 and IL-6 production were significantly suppressed in TLR4 down-regulated cells. Taken together, calprotectin induces IL-6 and MCP-1 production in HGFs via TLR4 signaling that involves MAPK and NF-B, resulting in the progression of periodontitis. J. Cell. Physiol. 232: 1862-1871, 2017. © 2016 Wiley Periodicals, Inc. (キーワード) Antigens, Neoplasm / Cell Line, Tumor / 細胞増殖·分化 (cell proliferation and differentiation) / Chemokine CCL2 / Down-Regulation / Fibroblasts / Gene Expression Regulation / Gingiva / Humans / Inflammation Mediators / Interleukin-6 / Intracellular Space / Leukocyte L1 Antigen Complex / Mitogen-Activated Protein Kinases / Models, Biological / NF-kappa B / Protein Kinase Inhibitors / RNA, Messenger / RNA, Small Interfering / 炎症性サイトカイン受容体の情報伝達 (Signal transduction of proinflammatory cytokine receptors) / Sulfonamides / Toll-Like Receptor 4 / Transfection (出版サイトへのリンク) ● Publication site (DOI): 10.1002/jcp.25724 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27925202 ● Summary page in Scopus @ Elsevier: 2-s2.0-85008440585 (DOI: 10.1002/jcp.25724, PubMed: 27925202, Elsevier: Scopus)

国際会議:

1.	Yasufumi Nishikawa, Koji Naruishi, Jun-ichi Kido and Hiromichi Yumoto : Diabetic high-glucose condition enhances calprotectin-induced inflammatory responses in gingival fibroblasts, 97th General Session & Exhibition of the International Association for Dental Research (Vancouver, Canada), Jun. 2019.
2.	Yuji Inagaki, Kohei Nonaka, Ryosuke Takagi, Yasufumi Nishikawa, Rie Kido, Eijiro Sakamoto, Koji Naruishi, Jun-ichi Kido and Hiromichi Yumoto : Kanroin Extracts Prevent Alveolar Bone Resorption in Experimental Rat Periodontitis, 96th General Session & Exhibition of the International Association of Dental Research, Jul. 2018.

国内講演発表:

1.	細木眞紀, 井上美穂, 岩浅匠真, 西川泰史, 秋月皆人, 吉原靖智, 大倉一夫, Zhang Yuehui, Yan Huijiao, Arief Waskitho, Rodrigo Shigeru Ofuchi, 大島正充, 鈴木善貴, 成谷美緒, 宮城麻友, Resmi Raju, 松香芳三 : 歯科補綴学(Ⅱ)実習における実習書改定後の検討in徳島, 大学教育カンファレンス, 2018年12月.
2.	稲垣裕司, 高木亮輔, 西川泰史, 木戸理恵, 坂本英次郎, 成石浩司, 木戸淳一, 湯本浩通 : 甘露飲エキスは破骨細胞と骨芽細胞の分化制御を介してラット実験的歯周炎の歯槽骨吸収を抑制する, 日本歯科保存学会2018年度秋季学術大会(第149回), 2018年11月.
3.	西川泰史, 成石浩司, 木戸淳一, 湯本浩通 : 歯肉線維芽細胞におけるカルプロテクチン誘導性炎症関連因子の産生に及ぼす高グルコースの影響, 第148回日本歯科保存学会秋季学術大会, 2018年6月.
4.	稲垣裕司, 野中康平, 高木亮輔, 西川泰史, 木戸理恵, 坂本英次郎, 成石浩司, 木戸淳一, 湯本浩通 : 甘露飲エキス配合製剤はラット実験的歯周炎において歯槽骨吸収を抑制する, 第61回春季日本歯周病学会学術大会, 2018年6月.

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

カルプロテクチン誘導性炎症関連因子の産生に及ぼす高グルコースの影響 (研究課題/領域番号: 19K21411 )
研究者番号（10823833）による検索