Miki Watanabe, Ryuji Nakagawa, Tomohiro Kohmoto, Takuya Naruto, Ken-ichi Suga, Aya Goji, Hideaki Horikawa, Kiyoshi Masuda, Shoji Kagami and Issei Imoto : Exome-first approach identified a novel gloss deletion associated with Lowe syndrome., Human Genome Variation, Vol.3, 16037, 2016.
(Summary)
Lowe syndrome (LS) is an X-linked disorder affecting the eyes, nervous system and kidneys, typically caused by missense or nonsense/frameshift OCRL mutations. We report a 6-month-old male clinically suspected to have LS, but without the Fanconi-type renal dysfunction. Using a targeted-exome sequencing-first approach, LS was diagnosed by the identification of a deletion involving 1.7 Mb at Xq25-q26.1, encompassing the entire OCRL gene and neighboring loci.
Myeloma (MM) cells and osteoclasts are mutually interacted to enhance MM growth while creating acidic bone lesions. Here, we explored acid sensing of MM cells and its role in MM cell response to acidic conditions. Acidic conditions activated the PI3K-Akt signaling in MM cells while upregulating the pH sensor transient receptor potential cation channel subfamily V member 1 (TRPV1) in a manner inhibitable by PI3K inhibition. The acid-activated PI3K-Akt signaling facilitated the nuclear localization of the transcription factor Sp1 to trigger the expression of its target genes, including TRPV1 and HDAC1. Consistently, histone deacetylation was enhanced in MM cells in acidic conditions, while repressing a wide variety of genes, including DR4. Indeed, acidic conditions deacetylated histone H3K9 in a DR4 gene promoter and curtailed DR4 expression in MM cells. However, inhibition of HDAC as well as either Sp1 or PI3K was able to restore DR4 expression in MM cells suppressed in acidic conditions. These results collectively demonstrate that acid activates the TRPV1-PI3K-Akt-Sp1 signaling in MM cells while inducing HDAC-mediated gene repression, and suggest that a positive feedback loop between acid sensing and the PI3K-Akt signaling is formed in MM cells, leading to MM cell response to acidic bone lesions.
Miki Watanabe, Yasunobu Hayabuchi, Akemi Ono, Takuya Naruto, Hideaki Horikawa, Kiyoshi Masuda, Tomohiro Kohmoto, Ryuji Nakagawa, Hiromichi Ito, Shoji Kagami and Issei Imoto : Detection of 1p36 deletion by clinical exome-first diagnostic approach., Human Genome Variation, Vol.3, 16006, 2016.
(Summary)
Although chromosome 1p36 deletion syndrome is considered clinically recognizable based on characteristic features, the clinical manifestations of patients during infancy are often not consistent with those observed later in life. We report a 4-month-old girl who showed multiple congenital anomalies and developmental delay, but no clinical signs of syndromic disease caused by a terminal deletion in 1p36.32-p36.33 that was first identified by targeted-exome sequencing for molecular diagnosis.
Tomasz D Pieczonka, Aneta Bragiel, Hideaki Horikawa, Kana Fukuta, Masami Yoshioka and Yasuko Ishikawa : Long-term administration of whey alters atrophy, gene expression profiles and dysfunction of salivar, Journal of Functional Foods, Vol.21, 349-358, 2016.
(Summary)
Salivary glands in elderly individuals commonly exhibit morphological changes and dysfunction resulting in xerostomia. Long-term (4-week) drinking of whey prevented and/or restored age-dependent decline of salivary volume and protein concentration, and atrophy of sublingual glands (SLGs) significantly in 88-week-old rats. The transcripts of 42 genes were up-regulated and 7 genes were down-regulated by more than 1.5-fold change with FDR0.1 after whey-drinking. The expression levels of genes associated with salivary proteins and tissue repair were significantly increased, while those associated with lipid metabolism were decreased. Venn diagram analysis revealed that expressions of 13 genes, including Tcfap2b and Abpa, were induced significantly by whey-drinking. Furthermore, secretory protein levels in SLGs and saliva were revealed by immunoblot analysis. This is the first study to report that whey-administration can prevent and/or restore age-dependent atrophy and functional decline of SLGs in rela
Yuko Toyoda, Sho Tabata, Jun Kishi, Takuya Kuramoto, Atsushi Mitsuhashi, Atsuro Saijo, Hiroshi Kawano, Hisatsugu Goto, Yoshinori Aono, Masaki Hanibuchi, Hideaki Horikawa, Toshihiro Nakajima, Tatsuhiko Furukawa, Saburo Sone, Shin-ichi Akiyama and Yasuhiko Nishioka : Thymidine Phosphorylase Regulates the Expression of CXCL10 in Rheumatoid Arthritis Fibroblast-like Synoviocytes, Arthritis & Rheumatology, Vol.66, No.3, 560-568, 2014.
(Summary)
Thymidine phosphorylase (TP) in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) is induced by tumor necrosis factor α (TNFα) and other cytokines that have been reported to be major inflammation mediators in RA. We previously demonstrated that TP plays an important role in angiogenesis and tumor growth, invasion, and metastasis. The aim of this study was to investigate whether the role of TP in the pathogenesis of RA is similar to its role in tumors. In FLS obtained from 2 patients with RA, the expression of TP, interferon-γ (IFNγ)-inducible protein 10 (CXCL10), and other cytokines was measured by quantitative real-time polymerase chain reaction, immunoblotting, and enzyme-linked immunosorbent assays. Microarray analysis was performed using FLS transfected with TYMP complementary DNA and treated with a TP inhibitor. The expression of TP in FLS was up-regulated by TNFα, interleukin-1β (IL-1β), IL-17, IFNγ, and lipopolysaccharide. Microarray analysis of FLS overexpressing TP identified CXCL10 as a thymidine phosphorylase-related gene. The expression of CXCL10 was induced by TNFα, and this induction was suppressed by TYMP small interfering RNA and TP inhibitor. Furthermore, the combination of TNFα and IFNγ synergistically augmented the expression of TP and CXCL10. TP-induced CXCL10 expression was suppressed by the antioxidant EUK-8. In the synovial tissue of patients with RA, TP levels were significantly correlated with CXCL10 expression. The combination of TNFα and IFNγ strongly induced the expression of thymidine phosphorylase in RA FLS. The induction of thymidine phosphorylase enhanced the expression of CXCL10, which may contribute to the Th1 phenotype and bone destruction observed in RA.
Tomasz Pieczonka, Aneta Bragiel, Hideaki Horikawa, Satsuki Yabuuchi, Yuko Takeuchi, Shizuko Yanagisawa, Masami Yoshioka and Yasuko Ishikawa : Whey-administration restores age-dependent atrophy and dysfunction of sublingual glands, 2016 General Session & Exhibition of the International Association for Dental Research, Seoul, Republic of Korea, Jun. 2016.