Tokushima University / Educator and Researcher Directory --- Kuramoto, Hitomi

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Research

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https://researchmap.jp/hk-tksm (researchmap)

Field of Study

○	歯科保存学

Subject of Study

○	歯髄炎の病態解析と新規歯髄保護療法の開発

Book / Paper

Academic Paper (Judged Full Paper):

1.	Hitomi Kuramoto, Tadashi Nakanishi, Daisuke Takegawa, MIEDA Katsuhiro and Keiichi Hosaka : Caffeic Acid Phenethyl Ester Induces Vascular Endothelial Growth Factor Production and Inhibits CXCL10 Production in Human Dental Pulp Cells, Current Issues in Molecular Biology, Vol.44, No.11, 5691-5699, 2022. (Summary) The survival rate of root non-vital teeth is lower than that of vital teeth. Therefore, to preserve the dental pulp is very important. The vascular endothelial growth factor (VEGF) is the most potent angiogenic factor involved in the vitality of dental pulp including reparative dentin formation. Caffeic acid phenethyl ester (CAPE) is a physiologically active substance of propolis and has some bioactivities such as anti-inflammatory effects. However, there are no reports on the effects of CAPE on dental pulp inflammation. In this study, we investigated the effects of CAPE on VEGF and inflammatory cytokine production in human dental pulp cells (HDPCs) to apply CAPE to an ideal dental pulp protective agent. We found that CAPE induced VEGF production from HDPCs. Moreover, CAPE induced the phosphorylation of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinases (ERK), and stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) in HDPCs. Furthermore, CAPE inhibited C-X-C motif chemokine ligand 10 (CXCL10) production in Pam3CSK4- and tumor necrosis factor-alpha (TNF-α)-stimulated HDPCs. In conclusion, these results suggest that CAPE might be useful as a novel biological material for vital pulp therapy by exerting the effects of VEGF production and anti-inflammatory activities. (Keyword) anti-inflammatory effect / caffeic acid phenethyl ester / dental pulp cells / pulpitis / vascular endothelial growth factor / vital pulp therapy (Tokushima University Institutional Repository) ● Metadata: 117775 (Link to Publication Site) ● Publication site (DOI): 10.3390/cimb44110385 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36421669 ● Search Scopus @ Elsevier (PMID): 36421669 ● Search Scopus @ Elsevier (DOI): 10.3390/cimb44110385 (Tokushima University Institutional Repository: 117775, DOI: 10.3390/cimb44110385, PubMed: 36421669)
2.	Hitomi Kuramoto, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Tadashi Nakanishi, Daisuke Takegawa, Ayako Washio, Chiaki Kitamura and Takashi Matsuo : Caffeic acid phenethyl ester (CAPE) induces VEGF expression and production in rat odontoblastic cells, BioMed Research International, Vol.Article ID 5390720, 2019. (Summary) Caffeic acid phenethyl ester (CAPE), the main component of propolis, has various biological activities including anti-inflammatory effect and wound healing promotion. Odontoblasts located in the outermost layer of dental pulp play crucial roles such as production of growth factors and formation of hard tissue termed reparative dentin in host defense against dental caries. In this study, we investigated the effects of CAPE on the upregulation of vascular endothelial growth factor (VEGF) and calcification activities of odontoblasts, leading to development of novel therapy for dental pulp inflammation caused by dental caries. CAPE significantly induced mRNA expression and production of VEGF in rat clonal odontoblast-like KN-3 cells cultured in normal medium or osteogenic induction medium. CAPE treatment enhanced nuclear factor-kappa B (NF-B) transcription factor activation, and furthermore, the specific inhibitor of NF-B significantly reduced VEGF production. The expression of VEGF receptor- (VEGFR-) 2, not VEGFR-1, was up regulated in KN-3 cells treated with CAPE. In addition, VEGF significantly increased mineralization activity in KN-3 cells. These findings suggest that CAPE might be useful as a novel biological material for the dental pulp conservative therapy. (Tokushima University Institutional Repository) ● Metadata: 114566 (Link to Publication Site) ● Publication site (DOI): 10.1155/2019/5390720 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31930126 ● Summary page in Scopus @ Elsevier: 2-s2.0-85077441042 (Tokushima University Institutional Repository: 114566, DOI: 10.1155/2019/5390720, PubMed: 31930126, Elsevier: Scopus)
3.	Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Hitomi Kuramoto, 鷲尾絢子, Tadashi Nakanishi, Daisuke Takegawa, 北村知昭 and Takashi Matsuo : Anti-inflammatory Effects of Catechin on Rat Odontoblastic Cells (KN-3), The Japanese Journal of Conservative Dentistry, Vol.60, No.5, 235-244, 2017. (Link to Publication Site) ● Publication site (DOI): 10.11471/shikahozon.60.235 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282680498132224 ● Search Scopus @ Elsevier (DOI): 10.11471/shikahozon.60.235 (DOI: 10.11471/shikahozon.60.235, CiNii: 1390282680498132224)

Review, Commentary:

1.	Daisuke Takegawa, Kazuhide Yonekura, Hitomi Kuramoto, Yumika Ida, Yuki Hosokawa, Ikuko Hosokawa, Yoshitaka Hosokawa, Toshiyuki Suge, Tadashi Nakanishi and Keiichi Hosaka : Clinical Key Points of Class II Direct Composite Restoration, Journal of Oral Health and Biosciences, Vol.36, No.1, 8-12, Sep. 2023. (Keyword) dental care (Tokushima University Institutional Repository) ● Metadata: 118637 (Link to Publication Site) ● Publication site (DOI): 10.20738/johb.36.1_8 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390016062665364224 ● Search Scopus @ Elsevier (DOI): 10.20738/johb.36.1_8 (Tokushima University Institutional Repository: 118637, DOI: 10.20738/johb.36.1_8, CiNii: 1390016062665364224)
2.	Hitomi Kuramoto and Tadashi Nakanishi : Possibility of applying caffeic acid phenethyl ester to the treatment of pulpitis, Medical Science Digest, Vol.48, No.11, 48-49, Oct. 2022. (Keyword) pulpitis / caffeic acid phenethyl ester / vascular endothelial growth factor / anti-inflammatory effect / vital pulp therapy
3.	Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa, Hitomi Kuramoto, Daisuke Takegawa, Tadashi Nakanishi and Takashi Matsuo : The Roles of Odontoblasts in Dental Pulp Innate Immunity, Japanese Dental Science Review, Vol.54, No.3, 105-117, Aug. 2018. (Summary) Odontoblasts located in the outermost layer of dental pulp form a natural barrier between mineralized tissues, dentin, and soft tissues, dental pulp, of the vital tooth, and they first recognize caries-related pathogens and sense external irritations. Therefore, odontoblasts possess a specialized innate immune system to fight oral pathogens invading into dentin. Generally, the rapid initial sensing of microbial pathogens, especially pathogen-associated molecular patterns (PAMPs) shared by microorganisms, are mediated by pattern recognition receptors (PRRs), such as Toll-like receptor and the nucleotide-binding oligomerization domain (NOD). The innate immune responses in odontoblasts initiated by sensing oral pathogens provide host protective events, such as inflammatory reactions, to produce a variety of pro-inflammatory mediators, including chemokines and cytokines. These attract various inflammatory cells and cause antibacterial reactions, such as the production of defensins, to kill microorganisms in the proximal region of the odontoblast layer. This review focuses on innate immunity, especially cellular and molecular mechanisms regarding the sensing of PAMPs from oral pathogens by PRRs, in odontoblasts and provides information for future studies for the development of novel therapeutic strategies, including diagnosis and treatment, to prevent exceeding dental pulp inflammation and preserve the dental pulp tissues. (Tokushima University Institutional Repository) ● Metadata: 112960 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jdsr.2018.03.001 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30128058 ● Summary page in Scopus @ Elsevier: 2-s2.0-85044379499 (Tokushima University Institutional Repository: 112960, DOI: 10.1016/j.jdsr.2018.03.001, PubMed: 30128058, Elsevier: Scopus)

Proceeding of International Conference:

1.	Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa, Hitomi Kuramoto, Katsuhiko Hirota, Yoichiro Miyake and Takashi Matsuo : Streptococcal histone-like DNA binding protein induces MINCLE expression on monocytes, 95th General Session & Exhibition of the International Association for Dental Research, San Francisco, Mar. 2017.
2.	Yuki Hosokawa, Hiromichi Yumoto, Kouji Hirao, Hitomi Kuramoto, Tadashi Nakanishi, Daisuke Takegawa and Takashi Matsuo : Anti-inflammatory effects of polyphenols on rat odontoblastic cells, 95th General Session & Exhibition of the International Association for Dental Research (San Francisco), Mar. 2017.

Proceeding of Domestic Conference:

1.	MIEDA Katsuhiro, Tadashi Nakanishi, Hitomi Kuramoto, Yoshitaka Hosokawa, Ikuko Hosokawa, Daisuke Takegawa and Keiichi Hosaka : Sudachitinがヒト歯髄細胞の炎症メディエーター発現に与える影響, 日本歯科保存学会 2023年度春季学術大会(第158回), Jun. 2023.
2.	Hitomi Kuramoto, Tadashi Nakanishi, Daisuke Takegawa, MIEDA Katsuhiro and Keiichi Hosaka : Caffeic acid phenethyl ester (CAPE) がマウス骨芽細胞様細胞の組織修復関連因子発現に与える影響, 日本歯科保存学会 2023年度春季学術大会(第158回), Jun. 2023.
3.	三枝克啓, Tadashi Nakanishi, Hitomi Kuramoto, Daisuke Takegawa and Keiichi Hosaka : Interleukin-22 がヒト歯髄細胞の炎症性メディエーター発現に及ぼす影響, 第4回象牙質歯髄治療学会学術大会, May 2023.
4.	Hitomi Kuramoto, Tadashi Nakanishi, Daisuke Takegawa, Yuki Hosokawa, Katsuhiro Mieda and Keiichi Hosaka : The effects of Caffeic acid phenethyl ester (CAPE) on VEGF production in dental pulp cells, 日本歯科保存学会2021年度秋季学術大会(第155回), Oct. 2021.
5.	Hitomi Kuramoto, Kouji Hirao, Yuki Hosokawa, Daisuke Takegawa, Hiromichi Yumoto and Tadashi Nakanishi : ラット象牙芽細胞(KN-3)におけるVEGFの石灰化誘導作用, 日本歯科保存学会 2019年度秋季学術大会(第151回) (福岡), Nov. 2019.
6.	Daisuke Takegawa, Tadashi Nakanishi, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Hitomi Kuramoto and Takashi Matsuo : Effects of interferon-γ on innate host responses in cultured odontoblast-like cells stimulated with NOD ligands, 第150回日本歯科保存学会 2019年度春季学術大会, Jun. 2019.
7.	Hitomi Kuramoto, Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa and Takashi Matsuo : Caffeic Acid Phenetyl Ester (CAPE)のラット象牙芽細胞(KN-3)におけるVEGF産生誘導機序の解析, 第40回日本歯内療法学会学術大会 (東京), Jun. 2019.
8.	Hitomi Kuramoto : ARONJの患者さんに対する歯科治療, 第18回西日本歯内療法学会, Sep. 2018.
9.	Hitomi Kuramoto, Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa, Tadashi Nakanishi, Daisuke Takegawa and Takashi Matsuo : Caffeic Acid Phenetyl Ester (CAPE)の象牙芽細胞と骨芽細胞におけるVEGF産生誘導機序の解析, 第148回日本歯科保存学会春季学術大会, Jun. 2018.
10.	Hitomi Kuramoto : The potential application of CAPE to conservative pulp therapy, Tokushima Bioscience Retreat 2017, Sep. 2017.
11.	Kouji Hirao, Hitomi Kuramoto, Hiromichi Yumoto, Yuki Hosokawa and Takashi Matsuo : プロポリス生理活性物質(CAPE; Caffeic Acid Phenetyl Ester)を用いた新規覆髄材の開発, 第17回西日本歯内療法学会研修会, Sep. 2017.
12.	Hitomi Kuramoto : 外傷後の上顎切歯に対する歯内療法の長期経過, 第17回西日本歯内療法学会, Sep. 2017.
13.	Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Hitomi Kuramoto and Takashi Matsuo : ヒト歯髄組織ならびにラット象牙芽細胞(KN-3)におけるMincle発現と象牙芽細胞での機能解析, 第38回日本歯内療法学会学術大会 & 第14回日韓合同歯内療法学会学術大会, Jul. 2017.
14.	Hitomi Kuramoto, Hiromichi Yumoto, Kouji Hirao, 細川由樹, Tadashi Nakanishi, Daisuke Takegawa and Takashi Matsuo : Caffeic Acid Phenethyl Ester (CAPE)のラット象牙芽細胞(KN-3)におけるVEGF産生に与える影響, 日本歯科保存学会2017年度春季学術大会(第146回), Jun. 2017.
15.	Hiromichi Yumoto, Kouji Hirao, 細川由樹, Hitomi Kuramoto and Takashi Matsuo : 40年以上前に外傷を受けた根未完成歯の根尖部に硬組織形成の誘導を試みた症例, 第16回西日本歯内療法学会研修会, Sep. 2016.
16.	Hitomi Kuramoto : 外傷後の上顎切歯に対する歯内療法, 第16回西日本歯内療法学会, Sep. 2016.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

VEGF産生と抗炎症効果を期待した根尖性歯周炎治療に対するCAPEの発展的研究 (Project/Area Number: 22K17041 )
Elucidation of the effect of CAPE on inflammation control and VEGF production in pulpitis lesions (Project/Area Number: 20K23084 )
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