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徳島大学先端酵素学研究所基幹研究部門
徳島大学研究クラスター群研究クラスター群 (インキュベーション)2202002 独自の解析技術と疾患科学の融合によるリボソーム創薬の創生
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研究活動

個人のホームページ

専門分野

分子生物学 (Molecular Biology), 分析化学 (Analytical Chemistry)

研究テーマ

最先端プロテオーム解析技術を駆使した細胞内タンパク質複合体の解析と疾患組織への応用 (リボソーム合成, プロテオミクス (proteomics), RNA (RNA), クロマトグラフィ (chromatography))

著書・論文

学術論文(審査論文):

1. Daishiro Kobayashi, Masaya Denda, Junya Hayashi, Kohta Hidaka, Yutaka Kohmura, Takaaki Tsunematsu, Kohei Nishino, Harunori Yoshikawa, OHKAWACHI Kento, Kiyomi Nigorikawa, Tetsuro Yoshimaru, Naozumi Ishimaru, Wataru Nomura, Toyomasa Katagiri, Hidetaka Kosako and Akira Otaka :
Sulfoxide-Mediated Cys-Trp-Selective Bioconjugation that Enables Protein Labeling and Peptide Heterodimerization,
ChemistryEurope, Vol.2, No.3-4, 2024.
(DOI: 10.1002/ceur.202400014)
2. C Joana R Faria, Michele Tinti, A Catarina Marques, Martin Zoltner, Harunori Yoshikawa, C Mark Field and David Horn :
An allele-selective inter-chromosomal protein bridge supports monogenic antigen expression in the African trypanosome.,
Nature Communications, Vol.14, No.1, 2023.
(徳島大学機関リポジトリ: 119445,   DOI: 10.1038/s41467-023-44043-y,   PubMed: 38081826)
3. Kohei Nishino, Harunori Yoshikawa, Kou Motani and Hidetaka Kosako :
Optimized Workflow for Enrichment and Identification of Biotinylated Peptides Using Tamavidin 2-REV for BioID and Cell Surface Proteomics.,
Journal of Proteome Research, 2022.
(DOI: 10.1021/acs.jproteome.2c00130,   PubMed: 35979633)
4. Takumi Maruhashi, Daisuke Sugiura, Il-mi Okazaki, Kenji Shimizu, K Takeo Maeda, Jun Ikubo, Harunori Yoshikawa, Katsumi Maenaka, Naozumi Ishimaru, Hidetaka Kosako, Tatsuya Takemoto and Taku Okazaki :
Binding of LAG-3 to stable peptide-MHC class II limits T cell function and suppresses autoimmunity and anti-cancer immunity.,
Immunity, Vol.55, No.5, 912-924.e8, 2022.
(DOI: 10.1016/j.immuni.2022.03.013,   PubMed: 35413245)
5. Harunori Yoshikawa, Kohei Nishino and Hidetaka Kosako :
Identification and validation of new ERK substrates by phosphoproteomic technologies including Phos-tag SDS-PAGE.,
Journal of Proteomics, Vol.258, 2022.
(DOI: 10.1016/j.jprot.2022.104543,   PubMed: 35231659)
6. Francisco Rodriguez-Algarra, E Robert A Seaborne, F Amy Danson, Selin Yildizoglu, Harunori Yoshikawa, Pik Pui Law, Zakaryya Ahmad, A Victoria Maudsley, Ama Brew, Nadine Holmes, Mateus Ochôa, Alan Hodgkinson, J Sarah Marzi, M Madapura Pradeepa, Matthew Loose, L Michelle Holland and K Vardhman Rakyan :
Genetic variation at mouse and human ribosomal DNA influences associated epigenetic states.,
Genome Biology, Vol.23, No.1, 2022.
(徳島大学機関リポジトリ: 116751,   DOI: 10.1186/s13059-022-02617-x,   PubMed: 35164830)
7. Harunori Yoshikawa, Ramasubramanian Sundaramoorthy, Daniel Mariyappa, Hao Jiang and I Angus Lamond :
Efficient and Rapid Analysis of Polysomes and Ribosomal Subunits in Cells and Tissues Using Ribo Mega-SEC.,
Bio-protocol, Vol.11, No.15, 2021.
(DOI: 10.21769/BioProtoc.4106,   PubMed: 34458400)
8. Alison Galloway, Aneesa Kaskar, Dimitrinka Ditsova, Abdelmadjid Atrih, Harunori Yoshikawa, Carolina Gomez-Moreira, Olga Suska, Marcin Warminski, Renata Grzela, I Angus Lamond, Edward Darzynkiewicz, Jacek Jemielity and H Victoria Cowling :
Upregulation of RNA cap methyltransferase RNMT drives ribosome biogenesis during T cell activation.,
Nucleic Acids Research, 2021.
(DOI: 10.1093/nar/gkab465,   PubMed: 34125914)
9. J Alejandro Brenes, Harunori Yoshikawa, Dalila Bensaddek, Bogdan Mirauta, Daniel Seaton, L Jens Hukelmann, Hao Jiang, Oliver Stegle and I Angus Lamond :
Erosion of human X chromosome inactivation causes major remodeling of the iPSC proteome.,
Cell Reports, Vol.35, No.4, 2021.
(徳島大学機関リポジトリ: 116512,   DOI: 10.1016/j.celrep.2021.109032,   PubMed: 33910018)
10. Keiichi Izumikawa, Hideaki Ishikawa, Harunori Yoshikawa, Sally Fujiyama, Akira Watanabe, Hiroyuki Aburatani, Hiroyuki Tachikawa, Toshiya Hayano, Yutaka Miura, Toshiaki Isobe, J Richard Simpson, Li Li, Jinrong Min and Nobuhiro Takahashi :
LYAR potentiates rRNA synthesis by recruiting BRD2/4 and the MYST-type acetyltransferase KAT7 to rDNA.,
Nucleic Acids Research, Vol.47, No.19, 10357-10372, 2019.
(DOI: 10.1093/nar/gkz747,   PubMed: 31504794)
11. Harunori Yoshikawa, Mark Larance, J Dylan Harney, Ramasubramanian Sundaramoorthy, Tony Ly, Tom Owen-Hughes and I Angus Lamond :
Efficient analysis of mammalian polysomes in cells and tissues using Ribo Mega-SEC.,
eLife, Vol.7, No.e36530, 2018.
(DOI: 10.7554/eLife.36530,   PubMed: 30095066)
12. Kifayathullah Liakath-Ali, W Eric Mills, Inês Sequeira, M Beate Lichtenberger, Oliveira Angela Pisco, H Kalle Sipilä, Ajay Mishra, Harunori Yoshikawa, Chih-Chien Colin Wu, Tony Ly, I Angus Lamond, M Ibrahim Adham, Rachel Green and M Fiona Watt :
An evolutionarily conserved ribosome-rescue pathway maintains epidermal homeostasis.,
Nature, Vol.556, No.7701, 376-380, 2018.
(DOI: 10.1038/s41586-018-0032-3,   PubMed: 29643507)
13. Keiichi Izumikawa, Yuko Nobe, Harunori Yoshikawa, Hideaki Ishikawa, Yutaka Miura, Hiroshi Nakayama, Takashi Nonaka, Masato Hasegawa, Naohiro Egawa, Haruhisa Inoue, Kouki Nishikawa, Koji Yamano, J Richard Simpson, Masato Taoka, Yoshio Yamauchi, Toshiaki Isobe and Nobuhiro Takahashi :
TDP-43 stabilises the processing intermediates of mitochondrial transcripts.,
Scientific Reports, Vol.7, No.1, 2017.
(徳島大学機関リポジトリ: 117768,   DOI: 10.1038/s41598-017-06953-y,   PubMed: 28794432)
14. Hideaki Ishikawa, Harunori Yoshikawa, Keiichi Izumikawa, Yutaka Miura, Masato Taoka, Yuko Nobe, Yoshio Yamauchi, Hiroshi Nakayama, J Richard Simpson, Toshiaki Isobe and Nobuhiro Takahashi :
Poly(A)-specific ribonuclease regulates the processing of small-subunit rRNAs in human cells.,
Nucleic Acids Research, Vol.45, No.6, 3437-3447, 2017.
(DOI: 10.1093/nar/gkw1047,   PubMed: 27899605)
15. Keiichi Izumikawa, Harunori Yoshikawa, Hideaki Ishikawa, Yuko Nobe, Yoshio Yamauchi, Sjaak Philipsen, J Richard Simpson, Toshiaki Isobe and Nobuhiro Takahashi :
Chtop (Chromatin target of Prmt1) auto-regulates its expression level via intron retention and nonsense-mediated decay of its own mRNA.,
Nucleic Acids Research, Vol.44, No.20, 9847-9859, 2016.
(DOI: 10.1093/nar/gkw831,   PubMed: 27683223)
16. Shigeko Sato, Hideaki Ishikawa, Harunori Yoshikawa, Keiichi Izumikawa, Richard J. Simpson and Nobuhiro Takahashi :
Collaborator of alternative reading frame protein (CARF) regulates early processing of pre-ribosomal RNA by retaining XRN2 (5 '-3 ' exoribonuclease) in the nucleoplasm,
Nucleic Acids Research, Vol.43, No.21, 10397-10410, 2015.
(DOI: 10.1093/nar/gkv1069,   PubMed: 26531822,   Elsevier: Scopus)
17. Masashi Saruhashi, Totan Ghosh Kumar, Kenta Arai, Yumiko Ishizaki, Kazuya Hagiwara, Kenji Komatsu, Yuh Shiwa, Keiichi Izumikawa, Harunori Yoshikawa, Taishi Umezawa, Yoichi Sakata and Daisuke Takezawa :
Plant Raf-like kinase integrates abscisic acid and hyperosmotic stress signaling upstream of SNF1-related protein kinase2.,
Proceedings of the National Academy of Sciences of the United States of America, Vol.112, No.46, E6388-96, 2015.
(DOI: 10.1073/pnas.1511238112,   PubMed: 26540727)
18. Harunori Yoshikawa, Hideaki Ishikawa, Keiichi Izumikawa, Yutaka Miura, Toshiya Hayano, Toshiaki Isobe, J Richard Simpson and Nobuhiro Takahashi :
Human nucleolar protein Nop52 (RRP1/NNP-1) is involved in site 2 cleavage in internal transcribed spacer 1 of pre-rRNAs at early stages of ribosome biogenesis.,
Nucleic Acids Research, Vol.43, No.11, 5524-5536, 2015.
(DOI: 10.1093/nar/gkv470,   PubMed: 25969445)
19. Naoki Miyazawa, Harunori Yoshikawa, Satomi Magae, Hideaki Ishikawa, Keiichi Izumikawa, Goro Terukina, Ai Suzuki, Sally Nakamura-Fujiyama, Yutaka Miura, Toshiya Hayano, Wataru Komatsu, Toshiaki Isobe and Nobuhiro Takahashi :
Human cell growth regulator Ly-1 antibody reactive homologue accelerates processing of preribosomal RNA.,
Genes to Cells, Vol.19, No.4, 273-286, 2014.
(DOI: 10.1111/gtc.12129,   PubMed: 24495227)
20. Hideaki Ishikawa, Yuko Nobe, Keiichi Izumikawa, Harunori Yoshikawa, Naoki Miyazawa, Goro Terukina, Natsuki Kurokawa, Masato Taoka, Yoshio Yamauchi, Hiroshi Nakayama, Toshiaki Isobe and Nobuhiro Takahashi :
Identification of truncated forms of U1 snRNA reveals a novel RNA degradation pathway during snRNP biogenesis.,
Nucleic Acids Research, Vol.42, No.4, 2708-2724, 2013.
(DOI: 10.1093/nar/gkt1271,   PubMed: 24311566)
21. Harunori Yoshikawa, Wataru Komatsu, Toshiya Hayano, Yutaka Miura, Keiichi Homma, Keiichi Izumikawa, Hideaki Ishikawa, Naoki Miyazawa, Hiroyuki Tachikawa, Yoshio Yamauchi, Toshiaki Isobe and Nobuhiro Takahashi :
Splicing factor 2-associated protein p32 participates in ribosome biogenesis by regulating the binding of Nop52 and fibrillarin to preribosome particles.,
Molecular & Cellular Proteomics, Vol.10, No.8, 2011.
(DOI: 10.1074/mcp.M110.006148,   PubMed: 21536856)
22. Sally Fujiyama-Nakamura, Harunori Yoshikawa, Keiichi Homma, Toshiya Hayano, Teruko Tsujimura-Takahashi, Keiichi Izumikawa, Hideaki Ishikawa, Naoki Miyazawa, Mitsuaki Yanagida, Yutaka Miura, Takashi Shinkawa, Yoshio Yamauchi, Toshiaki Isobe and Nobuhiro Takahashi :
Parvulin (Par14), a peptidyl-prolyl cis-trans isomerase, is a novel rRNA processing factor that evolved in the metazoan lineage.,
Molecular & Cellular Proteomics, Vol.8, No.7, 1552-1565, 2009.
(DOI: 10.1074/mcp.M900147-MCP200,   PubMed: 19369196)

学術論文(紀要・その他):

1. Daishiroh Kobayashi, Masaya Denda, JUNYA Hayashi, Kohta Hidaka, Yutaka Kohmura, Takaaki Tsunematsu, Kohei Nishino, Harunori Yoshikawa, OHKAWACHI Kento, Kiyomi Nigorikawa, Tetsuro Yoshimaru, Naozumi Ishimaru, Nomura Wataru, Toyomasa Katagiri, Hidetaka Kosako and Akira Otaka :
Sulfoxide-mediated Cys-Trp-selective bioconjugation that enables protein labeling and peptide heterodimerization,
ChemRxiv, 2024.
(DOI: 10.26434/chemrxiv-2024-tkv7w-v2)

総説・解説:

1. 𠮷川 治孝 :
co-fractionation MS 新たな網羅的タンパク質複合体解析法,
実験医学別冊 決定版 質量分析活用スタンダード, 293-299, 2023年9月.
2. 𠮷川 治孝, Lamond I. Angus :
Ribo Mega-SEC:サイズ排除クロマトグラフィーによる簡便なリボソームの分離法,
日本プロテオーム学会誌, Vol.5, No.1, 13-22, 2020年8月.
(DOI: 10.14889/jpros.5.1_13,   CiNii: 1390566775161618432)
3. 高橋 信弘, 𠮷川 治孝, 泉川 桂一, 石川 英明 :
プロテオミクスの手法によるヒト細胞リボソーム⽣合成経路とその制御機構の解明,
日本プロテオーム学会誌, Vol.2, No.1, 7-15, 2018年5月.
(DOI: 10.14889/jpros.2.1_7,   CiNii: 1390282681206333696)
4. 𠮷川 治孝, 泉川 桂一, 石川 英明, 高橋 信弘 :
リボソームの機能調節と疾患 II.リボソームRNAの転写後修飾とアセンブリー II‐1 リボソームサブユニット生合成の調節,
生化学, Vol.85, No.10, 861-870, 2013年10月.

国際会議:

1. Harunori Yoshikawa :
Efficient analysis of translation-active ribosomes in cells and tissues,
The 16th International Symposium of the Institute Network for Biomedical Sciences & KEY FORUM 2021 International Symposium, online, Kumamoto, Japan, Nov. 2021.
2. Harunori Yoshikawa and AI Lamond :
Full Spectrum Complexome Analyses: Towards the comprehensive characterisation of cellular protein complexes by Size Exclusion Chromatography,
British Society for Proteome Research Annual Scientific Meeting, Southampton, UK, Jul. 2019.
3. Harunori Yoshikawa, K Kilgour and AI Lamond :
Full spectrum complexome analyses: Towards the comprehensive characterisation of cellular protein complexes by SEC-uHPLC,
EMBLWellcome Genome Campus Conference: Proteomics in Cell Biology and Disease Mechanisms, Heidelberg, Germany, Feb. 2019.

国内講演発表:

1. 常松 貴明, 𠮷川 治孝, 永尾 瑠, 松澤 鎮史, 大塚 邦紘, 牛尾 綾, 石丸 直澄 :
がん特殊化リボソームの同定と機能解析,
第113回日本病理学会総会, 2024年3月.
2. 𠮷川 治孝 :
定量プロテオミクスによる 核⼩体リボソーム⽣合成過程の解明,
日本プロテオーム学会2023年大会 JPrOS2023 (21st JHUPO), 2023年7月.
3. 𠮷川 治孝 :
Co-Fractionation MS(CF-MS)による細胞内巨大タンパク質複合体の解析,
第23回日本蛋白質科学会年会, 2023年7月.
4. 𠮷川 治孝 :
Co-Fractionation MSによる細胞内巨大タンパク質複合体の解析,
第71回質量分析総合討論会, 2023年5月.
5. 𠮷川 治孝 :
核小体ヒトプレリボソームの新規分離法が切り拓く リボソーム合成因子の網羅的解析,
第45回 日本分子生物学会年会, 2022年12月.
6. 𠮷川 治孝, 小迫 英尊 :
Protein Correlation Profilingによる細胞内巨大タンパク質複合体の解析,
日本プロテオーム学会2022年大会, 2022年8月.
7. 𠮷川 治孝 :
Towards the comprehensive characterisation of cellular protein complexes by Size Exclusion Chromatography,
日本プロテオーム学会2019年大会・第70回日本電気泳動学会総会, 2019年7月.
8. 𠮷川 治孝 :
p32, FBL and Nop52 are involved in splitting step of pre-90S into pre-40S and pre-60S particles during human ribosome biogenesis,
Ribosome Meeting, 2012年3月.

その他・研究会:

1. 𠮷川 治孝 :
細胞内タンパク質合成装置リボソームの効率的な解析法 Ribo Mega-SECの確立とその応用,
徳島大学大学院医歯薬学研究部 2022年度骨・筋とCaクラスター・ミニリトリート, 2023年2月.
2. Harunori Yoshikawa :
Faster and more efficient approach for isolating ribosomes and nucleolar pre-ribosomal particles,
RNA public seminar series, The Centre for Integrative Biology of Toulouse (CBI), France, online, Apr. 2022.
3. 𠮷川 治孝 :
サイズ排除クロマトグラフィーを活用した簡便なリボソームの分離法,
第45回先端酵素学研究所セミナー, 2022年1月.
4. Harunori Yoshikawa :
Faster, more efficient and convenient approach for translation and ribosome biogenesis study,
Institute for Cell & Molecular BioSciences, Newcastle University, UK, Oct. 2019.

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

  • 相互作用タンパク質の同定・トポロジー解析を指向した光活性型クロスリンカーの開発 (研究課題/領域番号: 24K09728 )
  • 糖情報の入力による植物免疫シグナルの制御機構の解明 (研究課題/領域番号: 24K01720 )
  • 定量プロテオミクスが紐解く新規タンパク質複合体によるHPV陽性癌の新たな病因論 (研究課題/領域番号: 23K24547 )
  • がん組織特異的な特殊化リボソーム解析法の確立とその分子的実体の解明 (研究課題/領域番号: 21KK0266 )
  • 体液のマルチオミクス解析による寿命を制御するポストリソソーム経路の解明 (研究課題/領域番号: 21H05147 )
  • ポストリソソーム生物学研究領域の創成支援 (研究課題/領域番号: 21H05144 )
  • 簡便なリボソームインタラクトーム解析法によるMyc依存的ながん原リボソームの同定 (研究課題/領域番号: 21K06138 )
  • 細胞増殖因子LYARをターゲットとした分子標的薬の探索 (研究課題/領域番号: 24241075 )
  • 研究者番号(60709567)による検索