徳島大学 / 教育研究者総覧 --- 加藤遼

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研究活動

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https://orcid.org/0000-0002-9134-1382 (ORCID)

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研究テーマ

著書・論文

学術論文(審査論文):

1.	Tatsuya Fukuta, Ryo Kato, Takuo Tanaka and Taka-aki Yano : Fabrication of Mie-resonant silicon nanoparticles using laser annealing for surface-enhanced fluorescence spectroscopy, Microsystems & Nanoengineering, Vol.10, No.45, 45, 2024. (要約) Silicon nanostructures with unique Mie resonances have garnered considerable attention in the field of nanophotonics. Here, we present a simple and efficient method for the fabrication of silicon (Si) nanoparticle substrates using continuous-wave (CW) laser annealing. The resulting silicon nanoparticles exhibit Mie resonances in the visible region, and their resonant wavelengths can be precisely controlled. Notably, laser-annealed silicon nanoparticle substrates show a 60-fold enhancement in fluorescence. This tunable and fluorescence-enhancing silicon nanoparticle platform has tremendous potential for highly sensitive fluorescence sensing and biomedical imaging applications. (出版サイトへのリンク) ● Publication site (DOI): 10.1038/s41378-024-00666-9 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 38560726 ● Summary page in Scopus @ Elsevier: 2-s2.0-85188861821 (DOI: 10.1038/s41378-024-00666-9, PubMed: 38560726, Elsevier: Scopus)
2.	Ryo Kato, Taguchi Koki, Uemura Takafumi, Taka-aki Yano, Petritz Andreas, Stadlober Barbara, Sekitani Tsuyoshi and Takuo Tanaka : Mid-Infrared Photothermal Imaging of Photochemically Patterned Polymer Gate Dielectrics for Organic Thin-Film Transistors, ACS Applied Electronic Materials, 2024. (出版サイトへのリンク) ● Publication site (DOI): 10.1021/acsaelm.4c00161 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-85188100687 (DOI: 10.1021/acsaelm.4c00161, Elsevier: Scopus)
3.	Ryo Kato, Maeda Kaisei, Taka-aki Yano, Tanaka Kan and Takuo Tanaka : Label-free visualization of photosynthetic microbial biofilms using mid-infrared photothermal and autofluorescence imaging, Analyst, Vol.148, No.24, 6241-6247, 2023. (要約) The formation of photosynthetic microbial biofilms comprising multispecies biomolecules, such as extracellular polymeric substances (EPSs), and microbial cells play pivotal roles in maintaining or stimulating their biological functions. Although there are numerous studies on photosynthetic microbial biofilms, the spatial distribution of EPS components that are vital for microbial biofilm formation, such as exopolysaccharides and proteins, is not well understood. Visualization of photosynthetic microbial biofilms requires label-free methods, because labelling EPSs results in structural changes or aggregation. Raman spectroscopy is useful for label-free visualization of biofilm constituents based on chemical contrast. However, interference resulting from the bright autofluorescence of photosynthetic molecules and the low detection efficiency of Raman scattering make visualization a challenge. Herein, we visualized photosynthetic microbial biofilms in a label-free manner using a super-resolution optical infrared absorption imaging technique, called mid-infrared photothermal (MIP) microscopy. By leveraging the advantages of MIP microscopy, such as its sub-micrometer spatial resolution, autofluorescence-free features, and high detection sensitivity, the distribution of cyanobacteria and their extracellular polysaccharides in the biofilm matrix were successfully visualized. This showed that cyanobacterial cells were aligned along acidic/sulfated polysaccharides in the extracellular environment. Furthermore, spectroscopic analyses elucidated that during formation of biofilms, sulfated polysaccharides initially form linear structures followed by entrapment of cyanobacterial cells. The present study provides the foundation for further studies on the formation, structure, and biological functions of microbial biofilms. (キーワード) Biofilms / Polysaccharides / Cyanobacteria / Microscopy / Optical Imaging (出版サイトへのリンク) ● Publication site (DOI): 10.1039/D3AN01453C (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37947037 ● Search Scopus @ Elsevier (PMID): 37947037 ● Search Scopus @ Elsevier (DOI): 10.1039/D3AN01453C (DOI: 10.1039/D3AN01453C, PubMed: 37947037)
4.	Ryo Kato, Taka-aki Yano and Takuo Tanaka : Single-cell infrared vibrational analysis by optical trapping mid-infrared photothermal microscopy, Analyst, Vol.148, No.6, 1285-1290, 2023. (要約) Single-cell analysis by means of vibrational spectroscopy combined with optical trapping is a reliable platform for unveiling cell-to-cell heterogeneities in vast populations. Although infrared (IR) vibrational spectroscopy provides rich molecular fingerprint information on biological samples in a label-free manner, its application with optical trapping has never been achieved due to weak gradient forces generated by the diffraction-limited focused IR beam and strong background of water absorption. Herein, we present single-cell IR vibrational analysis that incorporates mid-infrared photothermal (MIP) microscopy with optical trapping. Optically trapped single polymer particles and red blood cells (RBCs) in blood could be chemically identified owing to their IR vibrational fingerprints. This single-cell IR vibrational analysis further allowed us to probe the chemical heterogeneities of RBCs originating from the variation in the intracellular characteristics. Our demonstration paves the way for the IR vibrational analysis of single cells and chemical characterization in various fields. (キーワード) Microscopy / Optical Tweezers / Spectrophotometry, Infrared / Polymers / Single-Cell Analysis (出版サイトへのリンク) ● Publication site (DOI): 10.1039/D2AN01917E (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36811918 ● Search Scopus @ Elsevier (PMID): 36811918 ● Search Scopus @ Elsevier (DOI): 10.1039/D2AN01917E (DOI: 10.1039/D2AN01917E, PubMed: 36811918)
5.	Ryo Kato, Taka-aki Yano, Takeo Minamikawa and Takuo Tanaka : High-sensitivity hyperspectral vibrational imaging of heart tissues by mid-infrared photothermal microscopy, Analytical Sciences, Vol.38, No.12, 1497-1503, 2022. (要約) Visualizing the spatial distribution of chemical compositions in biological tissues is of great importance to study fundamental biological processes and origin of diseases. Raman microscopy, one of the label-free vibrational imaging techniques, has been employed for chemical characterization of tissues. However, the low sensitivity of Raman spectroscopy often requires a long acquisition time of Raman measurement or a high laser power, or both, which prevents one from investigating large-area tissues in a nondestructive manner. In this work, we demonstrated chemical imaging of heart tissues using mid-infrared photothermal (MIP) microscopy that simultaneously achieves the high sensitivity benefited from IR absorption of molecules and the high spatial resolution down to a few micrometers. We successfully visualized the distributions of different biomolecules, including proteins, phosphate-including proteins, and lipids/carbohydrates/amino acids. Further, we experimentally compared MIP microscopy with Raman microscopy to evaluate the sensitivity and photodamage to tissues. We proved that MIP microscopy is a highly sensitive technique for obtaining vibrational information of molecules in a broad fingerprint region, thereby it could be employed for biological and diagnostic applications, such as live-tissue imaging. (キーワード) Microscopy / Spectrum Analysis, Raman / Vibration / Proteins / Lasers (出版サイトへのリンク) ● Publication site (DOI): 10.1007/s44211-022-00182-8 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36070070 ● Search Scopus @ Elsevier (PMID): 36070070 ● Search Scopus @ Elsevier (DOI): 10.1007/s44211-022-00182-8 (DOI: 10.1007/s44211-022-00182-8, PubMed: 36070070)
6.	Ryo Kato, Toki Moriyama, Takayuki Umakoshi, Taka-aki Yano and Prabhat Verma : Ultrastable tip-enhanced hyperspectral optical nano-imaging for defect analysis of large-sized WS2 layers, Science Advances, Vol.8, No.28, 2022. (徳島大学機関リポジトリ) ● Metadata: 117851 (出版サイトへのリンク) ● Publication site (DOI): 10.1126/sciadv.abo4021 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-85134651863 (徳島大学機関リポジトリ: 117851, DOI: 10.1126/sciadv.abo4021, Elsevier: Scopus)
7.	Ryo Kato, Mitsuhiro Uesugi, Yoshie Komatsu, Fusatoshi Okamoto, Takuo Tanaka, fumihisa Kitawaki and Taka-aki Yano : Highly Stable Polymer Coating on Silver Nanoparticles for Efficient Plasmonic Enhancement of Fluorescence, ACS Omega, 4286-4292, 2022. (徳島大学機関リポジトリ) ● Metadata: 117207 (出版サイトへのリンク) ● Publication site (DOI): 10.1021/acsomega.1c06010 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-85124952347 (徳島大学機関リポジトリ: 117207, DOI: 10.1021/acsomega.1c06010, Elsevier: Scopus)
8.	Ryo Kato, Taka-aki Yano and Takuo Tanaka : Multi-modal vibrational analysis of blend polymers using mid-infrared photothermal and Raman microscopies, Vibrational Spectroscopy, Vol.118, No.1, 2022. (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.vibspec.2021.103333 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-85123212071 (DOI: 10.1016/j.vibspec.2021.103333, Elsevier: Scopus)
9.	Takuo Tanaka, Taka-aki Yano and Ryo Kato : Nanostructure-enhanced infrared spectroscopy, Nanophotonics, Vol.11, No.11, 2541-2561, 2021. (徳島大学機関リポジトリ) ● Metadata: 117245 (出版サイトへのリンク) ● Publication site (DOI): 10.1515/nanoph-2021-0661 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-85120993492 (徳島大学機関リポジトリ: 117245, DOI: 10.1515/nanoph-2021-0661, Elsevier: Scopus)

総説・解説:

1.	加藤遼, 馬越貴之, バルマプラブハット : チップ増強ラマン分光法による二次元ナノ材料の表面構造解析, フォトニクスニュース, Vol.9, No.2, 103, 2023年12月.

国際会議:

1.	Ryo Kato, Taka-aki Yano and Takuo Tanaka : Ultrastable tip-enhanced Raman spectroscopic imaging of 2D material systems, SPIE Optics Photonics, San Diego,USA, Aug. 2023.
2.	Ryo Kato : Engineering of nanophotonic metamaterials with two dimensional nanomaterials for enhanced optical spectroscopy, A3 Metamaterials Forum 2023, Kyoto,Japan, Jun. 2023.
3.	Ryo Kato, Taka-aki Yano and Takuo Tanaka : Mid-infrared photothermal spectroscopy and imaging for biochemical analysis, Colloquium Spectroscopicum Internationale XLIII, Tokushima,Japan, Jun. 2023.
4.	Taka-aki Yano and Ryo Kato : All-dielectric metamaterial reflectors for highly-sensitive infrared spectroscopy, A3 Metamaterials Forum, Seoul,Korea, Jun. 2022.
5.	Taka-aki Yano, Ryo Kato and Takuo Tanaka : Active plasmonic vibrational spectroscopy of single-molecular chemical reactions, SPIE Optics + Photonics 2022, San Diego, Jun. 2022.
6.	Akari Okimura, Ryo Kato, Taka-aki Yano and Takuo Tanaka : Tip-enhanced Raman analysis of inorganic-binding peptide molecules, SPIE Photonics West 2022 On Demand, Feb. 2022. (出版サイトへのリンク) ● Publication site (DOI): 10.1117/12.2608429 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1117/12.2608429 (DOI: 10.1117/12.2608429)
7.	Ryo Kato, Taka-aki Yano and Takuo Tanaka : Multimodal vibrational imaging based on mid-infrared photothermal microscopy, SPIE Photonics West 2022 On Demand, Online, Feb. 2022.
8.	Taichi Hara, Ryo Kato, Taka-aki Yano and Takuo Tanaka : Metasurface-based perfect reflectors for highly-sensitive infrared spectroscopy, SPIE Photonics West 2022 On Demand, Feb. 2022.
9.	Mitsuhiro Uesugi, Yoshie Komatsu, Fusatoshi Okamoto, Ryo Kato, Takuo Tanaka, Taka-aki Yano and Fumihisa Kitawaki : Highly-stable Polymer Coating on Metallic Nanoparticles for Plasmonic Fluorescence Enhancement, The 9th International Symposium on Surface Science (ISSS-9), Dec. 2021.

国内講演発表:

1.	加藤遼 : バイオ・メディカル応用に向けたプラズモニック超解像ラマン顕微鏡の開発, 第47回レーザ顕微鏡研究会&シンポジウム, 2022年11月.
2.	藤原將行, 宮坂禎也, 小野雅之, 加藤遼, 田中拓男, 齋藤敦, 長谷川祐一, 糸長誠, 矢野隆章 : プラズモニック構造を用いたデジタル分光バイオセンシング, 医用分光学研究会第20回年会, 2022年10月.
3.	藤原將行, 宮坂禎也, 小野雅之, 加藤遼, 加治佐平, 安井武史, 田中拓男, 齋藤敦, 長谷川祐一, 糸長誠, 矢野隆章 : 金ナノ粒子-金ナノホール型ハイブリッド構造のプラズモン共鳴特性とデジタル比色センシング応用, 第83回応用物理学会秋季学術講演会, 23a-A101-4, 2022年9月.
4.	沖村あかり, 加藤遼, 矢野隆章, 田中拓男 : 無機材料認識ペプチド分子のギャップモード探針増強ラマン分光, 第69回応用物理学会春季学術講演会, 2022年3月.
5.	原太一, 加藤遼, 矢野隆章, 田中拓男 : 中赤外誘電体メタマテリアルの開発と高感度赤外分光への応用, 第69回応用物理学会春季学術講演会, 2022年3月.
6.	沖村あかり, 加藤遼, 矢野隆章, 田中拓男 : ギャップモード探針増強ラマン分光法を用いた無機材料認識ペプチドの単一分子分光ギャップモード探針増強ラマン分光法を用いた無機材料認識ペプチドの単一分子分光, 2021年度日本分光学会年次講演会, 2021年10月.
7.	原太一, 加藤遼, 矢野隆章, 田中拓男 : 中赤外誘電体メタマテリアルの開発と高感度赤外分光への応用, 2021年度日本分光学会年次講演会, 2021年10月.
8.	Taichi Hara, Ryo Kato, Taka-aki Yano and Takuo Tanaka : Perfect dielectric-metamaterial reflectors for highly-sensitive infrared spectroscopy, Optics and Photonics Japan 2021, Oct. 2021.
9.	Akari Okimura, Ryo Kato, Taka-aki Yano and Takuo Tanaka : Tip-enhanced Raman spectroscopy of single biomolecule, Optics and Photonics Japan 2021, Oct. 2021.
10.	小松由枝, 上杉充弘, 岡本房俊, 加藤遼, 田中拓男, 矢野隆章, 北脇文久 : 金属ナノ粒子の安定的高分子被膜とプラズモン蛍光増強センサーへの応用, 日本分析化学会第70年会, 2021年9月.

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

二次元材料・生体分子界面におけるナノ物性の解明と局所制御 (研究課題/領域番号: 24K17654 )
中赤外光熱変調散乱計測による生体化学イメージング (研究課題/領域番号: 23H04139 )
探針増強ラマン散乱・赤外吸収計測による生体ダイナミクスの解明 (研究課題/領域番号: 22K14650 )
生体試料の分子結合を網羅計測する超解像ラマン・赤外分光技術の創出 (研究課題/領域番号: 21K20503 )
研究者番号（90910513）による検索