Tokushima University / Educator and Researcher Directory --- Shiota, Hiroshi

Field of Study

Subject of Study

Book / Paper

Book:

1.	Kayo Shinomiya and Hiroshi Shiota : 薬物選択の論点, KANEHARA & CO., LTD., Tokyo, Feb. 2006.
2.	Hiroshi Shiota and Hiroshi Eguchi : 眼科検査法ハンドブック, IGAKU-SHOIN Ltd.Tokyo,Japan, Tokyo, Jun. 2005.
3.	Hiroshi Eguchi and Hiroshi Shiota : 眼科検査法ハンドブック第4版, IGAKU-SHOIN Ltd.Tokyo,Japan, Tokyo, Jun. 2005. (Keyword) 検体採取 / 眼表面 / 感染性眼疾患 / 染色法 / 蛍光抗体法
4.	Hiroshi Shiota and Shino Hayashi : 研修医のための感染症クリニカルガイダンス, ヴァンメディカル, Tokyo, Apr. 2005. (Summary) 6·12 眼科系
5.	Kayo Shinomiya and Hiroshi Shiota : Ocular side effects of oral drugs, KANEHARA & CO., LTD., Tokyo, Feb. 2005. (Keyword) side effects / adverse drug reactions / oral drug
6.	Hiroshi Shiota and Eiji Kudo : 眼科検査ガイド, 文光堂, Tokyo, Nov. 2004. (Summary) 角結膜疾患微生物検査真菌検査
7.	Fusao Ota, Hiroshi Shiota and 斉藤厚 : 感染症診療のコツと落とし穴斎藤厚/編, Nakayama-Shoten Co., Ltd., Tokyo, Sep. 2004. (Keyword) 真菌 / 酵母 / 角膜真菌症 / カラーボタン / Fusarium solani
8.	Hiroshi Shiota : ICDテキストプラクティカルな病院感染制御, Medicus SHUPPAN, Suita, Apr. 2004. (Summary) 眼感染症とICD
9.	Kayo Shinomiya and Hiroshi Shiota : 月刊眼科診療プラクティス 92．ウイルス性眼疾患の診療, 文光堂, Tokyo, Feb. 2003.
10.	Hiroshi Eguchi and Hiroshi Shiota : 治療的角膜移植の適応と問題点, 文光堂, Tokyo, Oct. 2002. (Keyword) 治療的角膜移植 / 全層角膜移植術
11.	秦聡 and Hiroshi Shiota : 新眼科スタッフハンドブック, Nankodo, Tokyo, Oct. 2002.
12.	Hiroshi Eguchi and Hiroshi Shiota : ウイルス学からみた医療の安全性, Medical Review Co.,Ltd, Osaka, Apr. 2002. (Keyword) 流行性角結膜炎 / 単純ヘルペス性角膜炎 / 水痘·帯状疱疹ウイルス感染症 / サイトメガロウイルス網膜炎
13.	Hiroshi Eguchi and Hiroshi Shiota : ウイルス学からみた医療の安全性, Medical Review Co.,Ltd, Osaka, Apr. 2002.
14.	Hiroshi Shiota and 賀島誠 : 月刊眼科診療プラクティス 79．角結膜疾患の薬物療法, 文光堂, Tokyo, Jan. 2002.
15.	Hiroshi Eguchi and Hiroshi Shiota : PLACTICAL OPHTHALMOLOGY 88 角膜内皮細胞:最近の知見と展望, 文光堂, Tokyo, 2002.
16.	Hiroshi Shiota : 眼科治療薬ハンドブック, 株式会社中外医学社, Tokyo, Apr. 2001.
17.	Kayo Shinomiya and Hiroshi Shiota : 9.角膜感染症 C.角膜ヘルペス, 株式会社中外医学社, Tokyo, Apr. 2001.
18.	Hiroshi Shiota : 新·抗菌薬の使い方選択理論とその実際, 三共, Apr. 2000. (Summary) 13 眼科領域の感染症
19.	大橋裕一, 望月學, Takeshi Naito and Hiroshi Shiota : 眼微生物事典, MEDICAL VIEW CO., LTD., Tokyo, May 1996.
20.	賀島誠 and Hiroshi Shiota : 眼科診療Q&A QUESTIONS&ANSWERS, 六法出版, Jan. 1988.
21.	Hiroshi Shiota and Hiroshi Eguchi : 眼科検査法ハンドブック, IGAKU-SHOIN Ltd.Tokyo,Japan, Tokyo, Jul. 1985. (Summary) ⅹⅹⅡ.検体採取法 A.微生物検査 1.表面，前房水

Academic Paper (Judged Full Paper):

1.	Tsuchigauchi Takeshi, Tetsuyuki Takahashi, Ohnishi Takamasa, Hirohisa Ogawa, Yoshimi Bando, Hisanori Uehara, Takizawa Tamatsu, Kaneda Shinya, Nakai Tokiko, Hiroshi Shiota and Keisuke Izumi : Low susceptibility to N-ethyl-N-nitrosourea-induced transplacental carcinogenesis in Long-Evans Cinnamon (LEC) rats, The Journal of Medical Investigation : JMI, Vol.56, No.3-4, 93-98, 2009. (Summary) The Long-Evans Cinnamon (LEC) rat, an animal model of Wilson's disease, is resistant to a variety of chemical carcinogenesis except liver and colon. In the present study, N-ethyl-N-nitrosourea (ENU)-induced transplacental carcinogenesis was examined in male and female LEC, Long-Evans Agouti (LEA), a sibling line of the LEC rat, and F344 rats (n=21). ENU was administered to pregnant rats as a single s.c. injection at a dose of 60 mg/kg body weight on the 17th day after conception. Cerebral/spinal gliomas and trigeminal/spinal nerve schwannomas developed in both LEA and F344 rats at 30 weeks of age, but no nervous system tumors developed in LEC rats, the difference being statistically significant. Lung adenomas also developed in LEA and F344 rats, but not in LEC rats. Semiquantitative RT-PCR demonstrated that metallothionein (MT)1a, MT2 and O(6)-methylguanine-DNA methyltransferase (MGMT) mRNA levels in the liver of LEC rats were higher than those in F344 and LEA rats. In addition, Western blot analysis showed that MT (MT1 plus MT2) in the liver of LEC rats was also higher than that in other strains. Present results suggest that high levels of MT and/or MGMT contribute to the resistance to nitrosamine-induced carcinogenesis in LEC rats. (Keyword) Animals / Base Sequence / Carcinogens / Ethylnitrosourea / Female / Liver / Lung Neoplasms / Male / Maternal-Fetal Exchange / Metallothionein / Nervous System Neoplasms / O(6)-Methylguanine-DNA Methyltransferase / Pregnancy / RNA, Messenger / Rats / Rats, Inbred F344 / Rats, Inbred LEC / Rats, Long-Evans / Species Specificity (Tokushima University Institutional Repository) ● Metadata: 111314 (Link to Publication Site) ● Publication site (DOI): 10.2152/jmi.56.93 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19763020 ● Search Scopus @ Elsevier (PMID): 19763020 ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.56.93 (Tokushima University Institutional Repository: 111314, DOI: 10.2152/jmi.56.93, PubMed: 19763020)
2.	Maki Nisino, Dai Mizuno, Kimoto Takashi, Shinahara Wakako, Fukuta Akiho, Tunetomo Takei, Kaori Sumida, Seiichiro Kitamura, Hiroshi Shiota and Hiroshi Kido : Influenza vaccine with Surfacten, a modified pulmonary surfactant, induces systemic and mucosal immune responses without side effects in minipigs, Vaccine, Vol.27, No.41, 5620-5627, 2009. (Summary) Immune responses and side effects of intranasally administered flu vaccine with the commercial product Surfacten, a modified bovine pulmonary surfactant, were investigated in minipigs. The use of minipigs was based on the anatomical resemblance of nasal lymph nodes, the principal antigen uptake site of respiratory mucosal immunity, between pig and human. Intranasal instillation of HA vaccine adjuvanted with Surfacten elicited significantly higher serum hemagglutination inhibition titers than the antigen alone, with wide cross-neutralizing activities of secretory IgA in nasal washes. No significant induction of inflammatory cytokines or migration of inflammatory cells was observed at the site of immunization or serum after the first immunization. These data suggest the potential usefulness of Surfacten for mucosal vaccination. (Keyword) Adjuvants, Immunologic / Administration, Intranasal / Animals / Antibodies, Viral / Cross Reactions / Hemagglutination Inhibition Tests / Immunity, Mucosal / Immunoglobulin A / Influenza A Virus, H3N2 Subtype / Influenza Vaccines / Nasal Mucosa / Neutralization Tests / Pulmonary Surfactants / Swine / Swine, Miniature / Vaccines, Subunit (Link to Publication Site) ● Publication site (DOI): 10.1016/j.vaccine.2009.07.024 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19647064 ● Search Scopus @ Elsevier (PMID): 19647064 ● Search Scopus @ Elsevier (DOI): 10.1016/j.vaccine.2009.07.024 (DOI: 10.1016/j.vaccine.2009.07.024, PubMed: 19647064)
3.	Asako Umehara, Mamiko Nishioka, Toshiyuki Obata, Yousuke Ebina, Hiroshi Shiota and Seiichi Hashida : A novel ultra-sensitive enzyme immunoassay for soluble human insulin receptor ectodomain and its measurement in urine from healthy subjects and patients with diabetes mellitus., Clinical Biochemistry, Vol.42, No.13-14, 1468-1475, 2009. (Summary) OBJECTIVE: For the early identification of patients at risk of developing diabetes mellitus, and to prevent the onset of diabetes by performing dietary counseling and exercise guidance, we have developed an ultra-sensitive immune complex transfer enzyme immunoassay (ICT-EIA) to measure soluble human insulin receptor ectodomain (sIRalpha) in urine which is collected non-invasively. DESIGN AND METHODS: We developed ICT-EIA for sIRalpha and measured urinary sIRalpha from 106 healthy volunteers, 35 obese volunteers and 42 patients with diabetes. RESULTS: The detection limit of ICT-EIA (0.04 pg/mL), using a urine sample of as little as 100 microL, was a few hundred-fold higher than that of conventional ELISA. Using ICT-EIA, the urinary sIRalpha level in patients with diabetes (9.7+/-20.1 pg/mg creatinine) was significantly higher than those in healthy volunteers (1.4+/-0.9; P<0.001). CONCLUSION: ICT-EIA for sIRalpha may be useful as a good marker for evaluating diabetes risk. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.clinbiochem.2009.06.014 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19560451 ● Search Scopus @ Elsevier (PMID): 19560451 ● Search Scopus @ Elsevier (DOI): 10.1016/j.clinbiochem.2009.06.014 (DOI: 10.1016/j.clinbiochem.2009.06.014, PubMed: 19560451)
4.	Kayo Shinomiya, Toshihiko Nagasawa, 木虎亜希子, 岡本里江 and Hiroshi Shiota : A case of congenital corneal anesthesia with the VACTERL association, Folia japonica de ophthalmologica clinica, Vol.2, No.4, 335-339, 2009. (Keyword) VACTERL連合 / 先天性角膜知覚麻痺 / 角膜障害 / 小児 / 自傷 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1523388080936047104 ● Summary page in Scopus @ Elsevier: 2-s2.0-84879359971 (CiNii: 1523388080936047104, Elsevier: Scopus)
5.	Yuka Nagasaki, Maki Moritani, Toshihito Tanahashi, Dai Osabe, Kyoko Nomura, Yuka Fujita, Parvaneh Keshavarz, Kiyoshi Kunika, Naoto Nakamura, Toshikazu Yoshikawa, Eiichiro Ichiishi, Hiroshi Shiota, Natsuo Yasui, Hiroshi Inoue and Mitsuo Itakura : Lack of association of genetic variation in chromosome region 15q14-22.1 with type 2 diabetes in a Japanese population., BMC Medical Genetics, Vol.9, 22, 2008. (Summary) BACKGROUND: Chromosome 15q14-22.1 has been linked to type 2 diabetes (T2D) and its related traits in Japanese and other populations. The presence of T2D disease susceptibility variant(s) was assessed in the 21.8 Mb region between D15S118 and D15S117 in a Japanese population using a region-wide case-control association test. METHODS: A two-stage association test was performed using Japanese subjects: The discovery panel (Stage 1) used 372 cases and 360 controls, while an independent replication panel (Stage 2) used 532 cases and 530 controls. A total of 1,317 evenly-spaced, common SNP markers with minor allele frequencies > 0.10 were typed for each stage. Captured genetic variation was examined in HapMap JPT SNPs, and a haplotype-based association test was performed. RESULTS: SNP2140 (rs2412747) (C/T) in intron 33 of the ubiquitin protein ligase E3 component n-recognin 1 (UBR1) gene was selected as a landmark SNP based on repeated significant associations in Stage 1 and Stage 2. However, the marginal p value (p = 0.0043 in the allelic test, OR = 1.26, 95% CI = 1.07-1.48 for combined samples) was weak in a single locus or haplotype-based association test. We failed to find any significant SNPs after correcting for multiple testing. CONCLUSION: The two-stage association test did not reveal a strong association between T2D and any common variants on chromosome 15q14-22.1 in 1,794 Japanese subjects. A further association test with a larger sample size and denser SNP markers is required to confirm these observations. (Keyword) Adult / Aged / Case-Control Studies / Chromosome Mapping / Chromosomes, Human, Pair 15 / Diabetes Mellitus, Type 2 / Female / Genetic Variation / Haplotypes / Humans / Japan / Linkage Disequilibrium / Male / Middle Aged / Polymorphism, Single Nucleotide (Tokushima University Institutional Repository) ● Metadata: 118434 (Link to Publication Site) ● Publication site (DOI): 10.1186/1471-2350-9-22 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18366806 ● Search Scopus @ Elsevier (PMID): 18366806 ● Search Scopus @ Elsevier (DOI): 10.1186/1471-2350-9-22 (Tokushima University Institutional Repository: 118434, DOI: 10.1186/1471-2350-9-22, PubMed: 18366806)
6.	Tsutomu YAMASHITA, Kayo Shinomiya, Satoe OKAMOTO, Akiko KITORA, Shino Hayashi, Hiroshi Shiota and So Hata : Visual function of congenital blepharoptosis, Folia japonica de ophthalmologica clinica, Vol.1, No.2, 161-165, 2008. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1522825130981529344 ● Summary page in Scopus @ Elsevier: 2-s2.0-84879395445 (CiNii: 1522825130981529344, Elsevier: Scopus)
7.	Toshihito Tanahashi, Keiko Shinohara, Parvaneh Keshavarz, Yuka Nagasaki, Katsuyuki Miyawaki, Kiyoshi Kunika, Maki Moritani, Naoto Nakamura, Toshikazu Yoshikawa, Hiroshi Shiota, Hiroshi Inoue and Mitsuo Itakura : The association of genetic variants in Krüppel-like factor 11 and Type 2 diabetes in the Japanese population., Diabetic Medicine, Vol.25, No.1, 19-26, 2008. (Summary) Krüppel-like factor 11 (KLF11) is a transcriptional factor of the zinc finger domain family that regulates the expression of insulin. In North European populations, its common functional variant Q62R (rs35927125) is a strong genetic factor for Type 2 diabetes (P = 0.00033, odds ratio for G allele = 1.29, 95% CI 1.12-1.49). We examined the contribution of KLF11 variants to the susceptibility to Type 2 diabetes in a Japanese population. By re-sequencing Japanese individuals (n = 24, partly 96), we screened all four exons, exon/intron boundaries and flanking regions of KLF11. Verified single nucleotide polymorphisms (SNPs) were genotyped in 731 initial samples (369 control and 362 case subjects). Subsequently, we tested for association in 1087 samples (524 control and 563 case subjects), which were collected in different districts of Japan from the initial samples. We identified eight variants, including a novel A/C variant on intron 3, but no mis-sense mutations. In an association study, we failed to find any significant result of SNPs (minor allele frequency 8.2-46.2%) after correcting for multiple testing. Similarly, no haplotypes were associated with Type 2 diabetes. It is notable that the G allele in rs35927125 was completely absent in 1818 Japanese individuals. Genetic variants in KLF11 are unlikely to have a major effect of Type 2 diabetes in the Japanese population, although they were significantly associated in North European populations. These observations might help to determine the role of KLF11 variants in Type 2 diabetes in different populations. (Keyword) Adult / analysis of variance / Asian Continental Ancestry Group / Cell Cycle Proteins / Diabetes Mellitus, Type 2 / female / Gene Frequency / Genetic Predisposition to Disease / Genotype / Humans / insulin / Japan / Linkage Disequilibrium / male / Middle Aged / Repressor Proteins (Tokushima University Institutional Repository) ● Metadata: 118435 (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1464-5491.2007.02315.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18199129 ● Search Scopus @ Elsevier (PMID): 18199129 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1464-5491.2007.02315.x (Tokushima University Institutional Repository: 118435, DOI: 10.1111/j.1464-5491.2007.02315.x, PubMed: 18199129)
8.	Takashi Katome, Takeshi Naito, Shingo Matsushita, Masaru Takebayashi, Hiroyuki Sato, Kayo Shinomiya, Hiroshi Shiota and 矢野雅彦 : 裂孔原性網膜剥離に対するインプラント手術後のバックル感染症の検討, Japanese Journal of Clinical Ophthalmology, Vol.62, No.5, 769-769772, 2008.
9.	Seiji Mitani, Shoichiro Fujisawa, Aya Mima, Hiroshi Shiota, Kenji Yanashima, Mitsue Takahara and Osamu Sueda : The Importance of Measuring Medical and Psychological Characteristics in Visibility Measurement of Persons with Low Visual Capability, Assistive Technology Research Series, Vol.20, 331-335, 2007. (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84865465319 (Elsevier: Scopus)
10.	Shino Hayashi, Kayo Shinomiya, 岡本里江, 木虎亜希子, 山下力 and Hiroshi Shiota : Review of ocular complications of epiblepharon, Japanese Journal of Clinical Ophthalmology, Vol.61, No.10, 1857-1860, 2007. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390846634872933120 ● Summary page in Scopus @ Elsevier: 2-s2.0-35748937949 (CiNii: 1390846634872933120, Elsevier: Scopus)
11.	Yukiko Yamashita, Hiroshi Inoue, Keshavarz Parvaneh, Katsuyuki Miyawaki, Yuka Nagasaki, Maki Moritani, Kiyoshi Kunika, Naoto Nakamura, Toshikazu Yoshikawa, Natsuo Yasui, Hiroshi Shiota, Toshihito Tanahashi and Mitsuo Itakura : SNPs in the KCNJ11-ABCC8 gene locus are associated with type 2 diabetes and blood pressure levels in the Japanese population., Journal of Human Genetics, Vol.52, No.10, 781-793, 2007. (Summary) Many genetic association studies support a contribution of genetic variants in the KCNJ11-ABCC8 gene locus to type 2 diabetes (T2D) susceptibility in Caucasians. In non-Caucasian populations, however, there have been only a few association studies, and discordant results were obtained. Herein, we selected a total of 31 SNPs covering a 211.3-kb region of the KCNJ11-ABCC8 locus, characterized the patterns of linkage disequilibrium (LD) and haplotype structure, and performed a case-control association study in a Japanese population consisting of 909 T2D patients and 893 control subjects. We found significant associations between eight SNPs, including the KCNJ11 E23K and ABCC8 S1369A variants, and T2D. These disease-associated SNPs were genetically indistinguishable because of the presence of strong LD, as found previously in Caucasians. For the KCNJ11 E23K variant, the most significant association was obtained under a dominant genetic model (OR 1.32, 95% CI 1.09-1.60, P = 0.004). A meta-analysis of East Asian studies, comprising a total of 3,357 T2D patients (77.4% Japanese) and 2,836 control subjects (77.8% Japanese), confirmed the significant role of the KCNJ11 E23K variant in T2D susceptibility. Furthermore, we found evidence suggesting that the KCNJ11 E23K genotype is independently associated with higher blood-pressure levels. (Keyword) ATP-Binding Cassette Transporters / Aged / Asian Continental Ancestry Group / blood pressure / Diabetes Mellitus, Type 2 / female / Humans / Japan / male / Middle Aged / Polymorphism, Single Nucleotide / Potassium Channels / Potassium Channels, Inwardly Rectifying / Receptors, Drug (Link to Publication Site) ● Publication site (DOI): 10.1007/s10038-007-0190-x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17823772 ● CiNii @ National Institute of Informatics (CRID): 1521417754939063936 ● Summary page in Scopus @ Elsevier: 2-s2.0-34848895423 (DOI: 10.1007/s10038-007-0190-x, PubMed: 17823772, CiNii: 1521417754939063936, Elsevier: Scopus)
12.	Dai Osabe, Toshihito Tanahashi, Kyoko Nomura, Shuichi Shinohara, Naoto Nakamura, Toshikazu Yoshikawa, Hiroshi Shiota, Parvaneh Keshavarz, Yuka Nagasaki, Kiyoshi Kunika, Maki Moritani, Hiroshi Inoue and Mitsuo Itakura : Evaluation of sample size effect on the identification of haplotype blocks., BMC Bioinformatics, Vol.8, 200, 2007. (Summary) Genome-wide maps of linkage disequilibrium (LD) and haplotypes have been created for different populations. Substantial sharing of the boundaries and haplotypes among populations was observed, but haplotype variations have also been reported across populations. Conflicting observations on the extent and distribution of haplotypes require careful examination. The mechanisms that shape haplotypes have not been fully explored, although the effect of sample size has been implicated. We present a close examination of the effect of sample size on haplotype blocks using an original computational simulation. A region spanning 19.31 Mb on chromosome 20q was genotyped for 1,147 SNPs in 725 Japanese subjects. One region of 445 kb exhibiting a single strong LD value (average \|D'\|; 0.94) was selected for the analysis of sample size effect on haplotype structure. Three different block definitions (recombination-based, LD-based, and diversity-based) were exploited to create simulations for block identification with theta value from real genotyping data. As a result, it was quite difficult to estimate a haplotype block for data with less than 200 samples. Attainment of a reliable haplotype structure with 50 samples was not possible, although the simulation was repeated 10,000 times. These analyses underscored the difficulties of estimating haplotype blocks. To acquire a reliable result, it would be necessary to increase sample size more than 725 and to repeat the simulation 3,000 times. Even in one genomic region showing a high LD value, the haplotype block might be fragile. We emphasize the importance of applying careful confidence measures when using the estimated haplotype structure in biomedical research. (Keyword) Chromosome Mapping / Chromosomes, Human, Pair 20 / Computer Simulation / Genetic Variation / Haplotypes / Humans / Linkage Disequilibrium / Models, Genetic / Models, Statistical / Sample Size / Sensitivity and Specificity (Tokushima University Institutional Repository) ● Metadata: 118432 (Link to Publication Site) ● Publication site (DOI): 10.1186/1471-2105-8-200 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17567919 ● Summary page in Scopus @ Elsevier: 2-s2.0-34547118094 (Tokushima University Institutional Repository: 118432, DOI: 10.1186/1471-2105-8-200, PubMed: 17567919, Elsevier: Scopus)
13.	Maki Moritani, Kyoko Nomura, Toshihito Tanahashi, Dai Osabe, Yuka Fujita, Shinohara Syuichi, Yuka Nagasaki, Parvaneh Keshavarz, Eiji Kudo, Naoto Nakamura, Toshikazu Yoshikawa, Eiichiro Ichiichi, Yoichiro Takata, Natsuo Yasui, Hiroshi Shiota, Kiyoshi Kunika, Hiroshi Inoue and Mitsuo Itakura : Genetic association of single nucleotide polymorphisms in endonuclease G-like 1 gene with type 2 diabetes in a Japanese population., Diabetologia, Vol.50, No.6, 1218-1227, 2007. (Summary) In order to identify type 2 diabetes disease susceptibility gene(s) in a Japanese population, we applied a region-wide case-control association test to the 20.4 Mb region between D3S1293 and D3S2319 on chromosome 3p24.3-22.1, supported by linkage to type 2 diabetes and its related traits in Japanese and multiple populations. We performed a two-stage association test using 1,762 Japanese persons with 485 gene-centric, evenly spaced, common single nucleotide polymorphism (SNP) markers with minor allele frequency >0.1. For mouse studies, total RNA was extracted from various organs of BKS.Cg-+Lepr(db)/+Lepr(db) and control mice, and from MIN6, NIH3T3 and C2C12 cell lines. We detected a landmark SNP375 (A/G) (rs2051211, p = 0.000046, odds ratio = 1.33, 95% CI 1.16-1.53) in intron 5 of the endonuclease G-like 1 (ENDOGL1) gene. Systematic dense SNPs approach identified a susceptibility linkage disequilibrium (LD) block of 116.5 kb by \|D'\|, an LD units map and a critical region of 2.1 kb by r (2) in ENDOGL1. A haplotype-based association test showed that an at-risk haplotype is associated with disease status (p = 0.00001). The expression of ENDOGL1 was rather ubiquitous with relatively abundant expression in the brain and also in a pancreatic islet beta cell line. Mouse Endogl1 expression increased in pancreatic islets of hyperglycaemic BKS.Cg-+Lepr(db)/+Lepr(db) mice compared with that in control mice. Based on the population genetics, fine mapping of LD block and haplotype analysis, we conclude that ENDOGL1 is a candidate disease-susceptibility gene for type 2 diabetes in a Japanese population. Further analysis in a larger sample size is required to substantiate this conclusion. (Keyword) Adult / Aged / Body Mass Index / Case-Control Studies / Diabetes Mellitus, Type 2 / Endodeoxyribonucleases / Endonucleases / Female / Genetic Predisposition to Disease / Hemoglobin A, Glycosylated / Humans / Japan / Male / Middle Aged / Polymorphism, Single Nucleotide / Reference Values (Link to Publication Site) ● Publication site (DOI): 10.1007/s00125-007-0631-2 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17415550 ● Search Scopus @ Elsevier (PMID): 17415550 ● Search Scopus @ Elsevier (DOI): 10.1007/s00125-007-0631-2 (DOI: 10.1007/s00125-007-0631-2, PubMed: 17415550)
14.	tsutomu Yamashita, So Hata, Akiko Kitora, Satoe Okamoto, Kayo Shinomiya and Hiroshi Shiota : 調節性内斜視の合併の有無による屈折異常弱視の比較検討, gankarinsyoiho, Vol.101, No.1, 64-68, 2007. (Keyword) 屈折性調節性内斜視 / 改善時期 / 立体視 / 外斜視 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1521699230351365376 (CiNii: 1521699230351365376)
15.	Kayo Shinomiya, nobuyuki itsuki, masanori kubo, masashi yamada, yaeko ohgi and Hiroshi Shiota : Analysis after removal cross-talk improves electro-oculogram usefulness, Folia Ophthalmologica Japonica, Vol.57, No.10, 739-743, 2006. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1573668925174885888 ● Summary page in Scopus @ Elsevier: 2-s2.0-33846850645 (CiNii: 1573668925174885888, Elsevier: Scopus)
16.	Toshihito Tanahashi, Dai Osabe, Kyoko Nomura, Syuichi Shinohara, Hitoshi Kato, Eiichiro Ichiishi, Naoto Nakamura, Toshikazu Yoshikawa, Yoichiro Takata, Tatsuro Miyamoto, Hiroshi Shiota, Parvaneh Keshavarz, Yuka Nagasaki, Kiyoshi Kunika, Maki Moritani, Hiroshi Inoue and Mitsuo Itakura : Association study on chromosome 20q11.21-13.13 locus and its contribution to type 2 diabetes susceptibility in Japanese., Human Genetics, Vol.120, No.4, 527-542, 2006. (Summary) Several linkage studies have predicted that human chromosome 20q is closely related to type 2 diabetes, but there is no clear evidence that certain variant(s) or gene(s) have strong effects on the disease within this region. To examine disease susceptibility variant in Japanese, verified SNPs from the databases, with a minor allele frequency larger than 0.15, were selected at 10-kb intervals across a 19.31-Mb region (20q11.21-13.13), which contained 291 genes, including hepatocyte nuclear factor 4alpha (HNF4alpha). As a result, a total of 1,147 SNPs were genotyped with TaqMan assay using 1,818 Japanese samples. By searching for HNF4alpha as a representative disease-susceptible gene, no variants of HNF4alpha were strongly associated with disease. To identify other genetic variant related with disease, we designed an extensive two-stage association study (725 first and 1,093 second test samples). Although SNP1146 (rs220076) was selected as a landmark within the 19.31 Mb region, the magnitude of the nominal P value (P = 0.0023) was rather weak. Subsequently, a haplotype-based association study showed that two common haplotypes were weakly associated with disease. All of these tests resulted in non-significance after adjusting for Bonferroni's correction and the false discovery rate to control for the impact of multiple testing. Contrary to the initial expectations, we could not conclude that certain SNPs had a major effect on this promising locus within the framework presented here. As a way to extend our observations, we emphasize the importance of a subsequent association study including replication and/or meta-analysis in multiple populations. (Keyword) Aged / Asian Continental Ancestry Group / Chromosome Mapping / Chromosomes, Human, Pair 20 / Diabetes Mellitus, Type 2 / Female / Gene Frequency / Genetic Predisposition to Disease / Genotype / Haplotypes / Hepatocyte Nuclear Factor 4 / Humans / Japan / Linkage Disequilibrium / Lod Score / Male / Middle Aged / Polymorphism, Single Nucleotide (Link to Publication Site) ● Publication site (DOI): 10.1007/s00439-006-0231-0 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16955255 ● Search Scopus @ Elsevier (PMID): 16955255 ● Search Scopus @ Elsevier (DOI): 10.1007/s00439-006-0231-0 (DOI: 10.1007/s00439-006-0231-0, PubMed: 16955255)
17.	Hitoshi Kato, Kyoko Nomura, Dai Osabe, Shuichi Shinohara, Osamu Mizumori, Rumi Katashima, Shoji Iwasaki, Koichi Nishimura, Masayasu Yoshino, Masato Kobori, Eiichiro Ichiishi, Naoto Nakamura, Toshikazu Yoshikawa, Toshihito Tanahashi, Parvaneh Keshavarz, Kiyoshi Kunika, Maki Moritani, Eiji Kudo, Kazue Tsugawa, Yoichiro Takata, Daisuke Hamada, Natsuo Yasui, Tatsuro Miyamoto, Hiroshi Shiota, Hiroshi Inoue and Mitsuo Itakura : Association of single nucleotide polymorphisms in the suppressor of cytokine signaling 2 (SOCS2) gene with type 2 diabetes in the Japanese., Genomics, Vol.87, No.4, 446-458, 2006. (Summary) Several previous linkage scans in type 2 diabetes (T2D) families indicated a putative susceptibility locus on chromosome 12q15-q22, while the underlying gene for T2D has not yet been identified. We performed a region-wide association analysis on 12q15-q22, using a dense set of >500 single-nucleotide polymorphisms (SNPs), in 1492 unrelated Japanese individuals enrolled in this study. We identified an association between T2D and a haplotype block spanning 13.6 kb of genomic DNA that includes the entire SOCS2 gene. Evolutionary-based haplotype analysis of haplotype-tagging SNPs followed by a "sliding window" haplotypic analysis indicated SNPs that mapped to the 5' region of the SOCS2gene to be associated with T2D with high statistical significance. The SOCS2 gene was expressed ubiquitously in human and murine tissues, including pancreatic beta-cell lines. Adenovirus-mediated expression of the SOCS2 gene in MIN6 cells or isolated rat islets significantly suppressed glucose-stimulated insulin secretion. Our data indicate that SOCS2 may play a role in susceptibility to T2D in the Japanese. (Keyword) Adenoviridae / Adult / Animals / Case-Control Studies / Cell Line / Cell Line, Tumor / Cells, Cultured / Chromosome Mapping / Chromosomes, Human, Pair 12 / Diabetes Mellitus, Type 2 / Female / Glucose / Haplotypes / Humans / Insulin / Islets of Langerhans / Japan / Linkage Disequilibrium / Male / Mice / Mice, Inbred C57BL / Mice, Transgenic / Middle Aged / Models, Genetic / Polymorphism, Single Nucleotide / Radioimmunoassay / Rats / Reverse Transcriptase Polymerase Chain Reaction / Suppressor of Cytokine Signaling Proteins (Link to Publication Site) ● Publication site (DOI): 10.1016/j.ygeno.2005.11.009 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16406727 ● Summary page in Scopus @ Elsevier: 2-s2.0-33645095759 (DOI: 10.1016/j.ygeno.2005.11.009, PubMed: 16406727, Elsevier: Scopus)
18.	Parvaneh Keshavarz, Hiroshi Inoue, Yukiko Yamashita, Kiyoshi Kunika, Toshihito Tanahashi, Naoto Nakamura, Toshikazu Yoshikawa, Natsuo Yasui, Hiroshi Shiota and Mitsuo Itakura : No evidence for association of ENPP1(PC-1)K121Q variant with risk of Type 2 diabetes in a Japanese population., Journal of Human Genetics, Vol.51, No.6, 559-566, 2006. (Summary) Ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1, also known as PC-1) inhibits insulin signal transduction pathway(s). Previous studies have demonstrated the K121Q variant of the ENPP1 gene to have a significant functional role in determining susceptibility to insulin resistance and type 2 diabetes (T2D). To assess whether the K121Q variant has any impact on T2D in Japanese, we undertook an extensive case-control association study using a total of 911 unrelated Japanese T2D patients and 876 control subjects. No significant difference was observed in either genotype distribution (P=0.95) or allele frequency (P=0.83) between T2D and control groups. Notably, the frequency of the ancestral Q121 allele, which is also present in other primates, was quite high in African-Americans, and showed a marked ethnic variation (77.3% in African-Americans, 16.7% in European Americans, 10.5% in Japanese and 4.2% in Han Chinese). Consequently, the pairwise F(ST )value (a classic measure of genetic distance between pairs of population) showed highly significant differentiations between African-American and non-African-American populations (F(ST)>0.3). Our results indicated that the K121Q variant of the ENPP1 gene has very little, if any, impact on T2D susceptibility in Japanese, but may play a role in the inter-ethnic variability in insulin resistance and T2D. (Keyword) African Americans / Alleles / Amino Acid Substitution / Asian Continental Ancestry Group / Case-Control Studies / Diabetes Mellitus, Type 2 / European Continental Ancestry Group / Female / Gene Frequency / Genetic Variation / Humans / insulin resistance / Japan / Male / Phosphoric Diester Hydrolases / Pyrophosphatases / Risk Factors (Link to Publication Site) ● Publication site (DOI): 10.1007/s10038-006-0399-0 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16607460 ● Summary page in Scopus @ Elsevier: 2-s2.0-33745477936 (DOI: 10.1007/s10038-006-0399-0, PubMed: 16607460, Elsevier: Scopus)
19.	Tsutomu Yamashita, Akiko Kitora, Satoe Okamoto, Kayo Shinomiya, So Hata and Hiroshi Shiota : Therapeutic outcome of hyperopic anisometropic amblyopia at Tokushima University Hospital, gankarinsyoiho, Vol.100, No.1, 54-57, 2006. (Keyword) 改善期間 / 3歳児健診 / 立体視 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1524232504560332544 (CiNii: 1524232504560332544)
20.	Kayo Shinomiya, akiko kitora, satoe okamoto, yaeko kubo and Hiroshi Shiota : 徳島大学における屈折異常弱視と不同視弱視の検討, Japanese Journal of Clinical Ophthalmology, Vol.59, No.10, 1755-1758, 2005. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520854805090639616 ● Summary page in Scopus @ Elsevier: 2-s2.0-27744431932 (CiNii: 1520854805090639616, Elsevier: Scopus)
21.	Tatsuro Miyamoto, Hiroshi Inoue, Yukiko Yamashita, Eiji Kudo, Takeshi Naito, Takako Mikawa, Yoichi Mikawa, Yasushi Isashiki, Dai Osabe, Shuichi Shinohara, Hiroshi Shiota and Mitsuo Itakura : Identification of a novel splice site mutation of the CSPG2 gene in a Japanese family with Wagner syndrome., Investigative Ophthalmology & Visual Science, Vol.46, No.8, 2726-2735, 2005. (Summary) This linkage study confirmed the genetic homogeneity of the Wagner syndrome. CSPG2 encodes versican, a large chondroitin sulfate proteoglycan, which, in vitreous, binds to hyaluronan and link protein and forms large aggregates that are important for maintaining structural integrity. Although the CSPG2 gene has been excluded as a candidate for causing Wagner syndrome, these data emphasize the necessity of further mutational screening in new families and careful functional characterization. (Keyword) Adolescent / Adult / children / Child, Preschool / Chondroitin Sulfate Proteoglycans / Chromosomes, Human, Pair 5 / Consanguinity / Eye Diseases / Female / Genetic Linkage / Genotype / Humans / Japan / Male / Mutation / Nerve Tissue Proteins / Pedigree / Phenotype / RNA Splice Sites / RNA, Messenger / Retinal Degeneration / Reverse Transcriptase Polymerase Chain Reaction / Sequence Analysis, DNA / Syndrome / Versicans / Visual Fields / Vitreous Body (Link to Publication Site) ● Publication site (DOI): 10.1167/iovs.05-0057 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16043844 ● Summary page in Scopus @ Elsevier: 2-s2.0-24644524228 (DOI: 10.1167/iovs.05-0057, PubMed: 16043844, Elsevier: Scopus)
22.	Aya Mima, Makoto Kajima, Takeshi Naito and Hiroshi Shiota : Review of endogenous uveitis at Tokushima University Hospital, Japanese Journal of Clinical Ophthalmology, Vol.59, No.7, 1067-1071, 2005. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520573330919279488 ● Summary page in Scopus @ Elsevier: 2-s2.0-23344434506 (CiNii: 1520573330919279488, Elsevier: Scopus)
23.	Shino Hayashi, Hiroshi Eguchi, 長澤利彦 and Hiroshi Shiota : Two Cases of Urrets-Zavalia Syndrome Following Deep Lamellar Keratoplasty for Corneal Leukoma, Journal of the Eye, Vol.22, No.2, 221-224, 2005. (Keyword) 角膜白斑 / 深層角膜移植術 / 不可逆性散瞳 / Urrets-Zavalia症候群 / cornel leukoma / deep lamellar keratoplasty / fixed and dilated pupil / Urrets-Zavalia syndrome (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520854805808587776 (CiNii: 1520854805808587776)
24.	Traian V Chirila, Mika Chirila, Yoshito Ikada, Hiroshi Eguchi and Hiroshi Shiota : A Historical Review of Artificial Cornea Research in Japan, --- Artificial Cornea in Japan ---, Japanese Journal of Ophthalmology, Vol.49, No.1, S1-S13, 2005. (Summary) Little is known or understood about the history of artificial cornea (keratoprosthesis) in Japan, mainly because of the unavailability and linguistic inaccessibility of the relevant publications. Rather strangely also, the Japanese scholars themselves have manifested only slight interest in reviewing the topic, even in their own language. As a result, the research on artificial cornea in Japan showed in fact the same pattern of historical development as that seen in the Western world. After audacious beginnings in the 19th century, an interlude of about half a century followed, when the interest of ophthalmologists was deflected to penetrating keratoplasty as a means to fight corneal blindness. The introduction in medical use of man-made polymeric materials in the 1950s triggered a revival of research on artificial cornea in Japan, and the next two decades were rich in ideas, achievements, and a plethora of reports, all published in Japanese. In the 1980s, the developmental work on keratoprosthesis became incorporated into the field of biomaterials, and the creation of materials able to mimic the corneal tissue was established as a leading concept, resulting in original designs with some sophistication. At the same time. the English language started to be used extensively for dissemination of results. These trends have continued to date. Based on rigorous documentation, we traced the history of research on artificial cornea in Japan from its origin to our time. (Keyword) Aritificial cornea / Japan / Historical review (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15717417 ● CiNii @ National Institute of Informatics (CRID): 1523106604884853504 ● Search Scopus @ Elsevier (PMID): 15717417 (PubMed: 15717417, CiNii: 1523106604884853504)
25.	Kayo Shinomiya, 岡本里江, 木虎亜希子, Toshihiko Nagasawa and Hiroshi Shiota : 3歳児健診での要精査児の受診結果と問題点, gankarinsyoiho, Vol.99, No.4, 326-329, 2005. (Keyword) 弱視 / 斜視 / 中断 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1523951030202609664 (CiNii: 1523951030202609664)
26.	久保弥栄子, 賀島誠, Takeshi Naito and Hiroshi Shiota : Vitreous surgery in a case of pit macular syndrome with macular hole, Ophthalmology, Vol.46, No.12, 1785-1790, 2004. (Keyword) 視神経乳頭小窩 / 黄斑円孔 / 硝子体手術 / pit macular syndrome / macular hole / vitrectomy (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1522543655299444608 (CiNii: 1522543655299444608)
27.	Yuki Hayashi, Naozumi Ishimaru, Rieko Arakaki, Shin-ichi Tsukumo, Hitomi Fukui, Kenji Kishihara, Hiroshi Shiota, Koji Yasutomo and Yoshio Hayashi : Effective Treatment of a Mouse Model of Sjogren's Syndrome With Eyedrop Administrasiton of Anti-CD4 Monoclonal Antibody, Arthritis and Rheumatism, Vol.50, No.9, 2903-2910, 2004. (Summary) To determine whether eyedrop administration of an anti-CD4 monoclonal antibody (mAb) is effective in the treatment of Sjögren's syndrome (SS) using a mouse model of the disease. The anti-CD4 mAb was administered daily into the eyes of mice with SS from ages 4 to 8 weeks or ages 10 to 12 weeks. During treatment, tear volume was monitored and after final treatment, histologic features of the lacrimal and salivary glands, the phenotypes and function of T cells, and serum titers of anti-alpha-fodrin antibody were examined. Eyedrop administration of anti-CD4 mAb before the onset of SS prevented the autoimmune pathology seen in the lacrimal glands but not that in the salivary glands. Furthermore, eyedrop administration of anti-CD4 mAb after the development of SS inhibited mononuclear cell infiltration and the destruction of parenchyma only in the lacrimal glands. Eyedrop administration of anti-CD4 mAb suppressed the local activation of CD4+ T cells rather than deleting CD4+ T cells, which reduced the expansion of pathologic CD4+ T cells against alpha-fodrin. These results demonstrate the remarkable efficacy of anti-CD4 mAb eyedrops in the treatment of SS eye symptoms, which illustrates a new antibody-based therapeutic strategy for patients with eye problems caused by SS as well as other diseases. (Keyword) Administration, Topical / Animals / Antibodies, Monoclonal / Antigens, CD4 / CD4-Positive T-Lymphocytes / Disease Progression / Dry Eye Syndromes / Female / Lacrimal Apparatus / Lymphocyte Activation / Mice / Models, Animal / Ophthalmic Solutions / salivary glands / Sjögren's syndrome / Treatment Outcome (Link to Publication Site) ● Publication site (DOI): 10.1002/art.20472 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15457459 ● Search Scopus @ Elsevier (PMID): 15457459 ● Search Scopus @ Elsevier (DOI): 10.1002/art.20472 (DOI: 10.1002/art.20472, PubMed: 15457459)
28.	Keiko Okamoto, 賀島誠, 美馬彩, Takeshi Naito and Hiroshi Shiota : A case of tamoxifen retinopathy, Japanese Journal of Clinical Ophthalmology, Vol.58, No.6, 987-990, 2004. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390002209941654656 ● Summary page in Scopus @ Elsevier: 2-s2.0-3142624621 (CiNii: 1390002209941654656, Elsevier: Scopus)
29.	Takashi Katome, Takeshi Naito, 賀島誠 and Hiroshi Shiota : Outocome of vitreous surgery for idiopathic macular hole with or without peeling of internal limiting membrane, Japanese Journal of Clinical Ophthalmology, Vol.58, No.6, 959-962, 2004. (Keyword) 硝子体切除術 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-3142516161 (Elsevier: Scopus)
30.	秦聡, 賀島誠, Takeshi Naito and Hiroshi Shiota : A case of endophthalmitis following vitreous surgery due to methicillin-resistant Staphylococcus aureus, Japanese Journal of Clinical Ophthalmology, Vol.58, No.5, 785-787, 2004. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390846634872191616 ● Summary page in Scopus @ Elsevier: 2-s2.0-3042593150 (CiNii: 1390846634872191616, Elsevier: Scopus)
31.	Yuki Hayashi, Shin-ichi Tsukumo, Hiroshi Shiota, Kenji Kishihara and Koji Yasutomo : Antigen-Specific T Cell Repertoire Modification of CD4⁺CD25⁺ Regulatory T Cells, The Journal of Immunology, Vol.172, No.9, 5240-5248, 2004. (Summary) T cell immune responses are regulated by the interplay between effector and suppressor T cells. Immunization with Ag leads to the selective expansion and survival of effector CD4(+) T cells with high affinity TCR against the Ag and MHC. However, it is not known if CD4(+)CD25(+) regulatory T cells (T(reg)) recognize the same Ag as effector T cells or whether Ag-specific TCR repertoire modification occurs in T(reg). In this study, we demonstrate that after a primary Ag challenge, T(reg) proliferate and TCR repertoire modification is observed although both of these responses were lower than those in conventional T cells. The repertoire modification of Ag-specific T(reg) after primary Ag challenge augmented the total suppressive function of T(reg) against TCR repertoire modification but not against the proliferation of memory CD4(+) T cells. These results reveal that T cell repertoire modification against a non-self Ag occurs in T(reg), which would be crucial for limiting excess primary and memory CD4(+) T cell responses. In addition, these studies provide evidence that manipulation of Ag-specific T(reg) is an ideal strategy for the clinical use of T(reg). (Keyword) Animals / Antigen Presentation / Antigens / CD4-Positive T-Lymphocytes / cell differentiation / cell division / Cells, Cultured / Columbidae / Complementarity Determining Regions / Cytochromes c / Epitopes, T-Lymphocyte / Immunization, Secondary / Immunologic Memory / Lymphocyte Count / Mice / Mice, Inbred A / Mice, Inbred C57BL / Receptors, Antigen, T-Cell, alpha-beta / T-Lymphocytes, Regulatory (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15100262 ● Search Scopus @ Elsevier (PMID): 15100262 (PubMed: 15100262)
32.	Mayumi Ishimaru, Makoto Kajima, Takeshi Naito and Hiroshi Shiota : Investigation of Rhegmatogenous Retinal Detachment in Children, Japanese Journal of Ophthalmic Surgery, Vol.17, No.2, 261-263, 2004. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1572543024987315200 (CiNii: 1572543024987315200)
33.	Takeo Oogi, So Hata, Makoto Kajima, Takeshi Naito and Hiroshi Shiota : Submacular neovascular membrane in Vogt-Koyanagi-Harada disease performed submacular surgery, Ophthalmology, Vol.46, No.1, 73-77, 2004. (Keyword) Harada disease / submaclar surgery / submaclar neovascular membrane (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1523106605078141056 (CiNii: 1523106605078141056)
34.	Kyoko Fuke, Takeshi Naito, Makoto Kajima and Hiroshi Shiota : Outcome of surgical removal of subchoroidal neovascular membrane in neovascular maculopathy with high myopia, gankarinsyoiho, Vol.97, No.9, 775-777, 2003. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1571980075771439104 (CiNii: 1571980075771439104)
35.	Takeshi Thuchigauchi, Kayo Shinomiya, Hiroshi Eguchi, Takeshi Naito, Hiroshi Shiota and 藤江準 : An atypical case of retinoschisis in posterior staphvloma, Ophthalmology, Vol.45, No.8, 1225-1227, 2003. (Keyword) posterior staphyloma / retinoschisis / vitrectomy (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1522825130751786880 (CiNii: 1522825130751786880)
36.	Kayo Shinomiya, Makoto Kajima, Hiroko Tajika, Hiroshi Shiota, Ryuji Nakagawa and Takahiko Saijyou : Renal failure caused by eyedrops containing phenylephrine in a case of retinopathy of prematurity, The Journal of Medical Investigation : JMI, Vol.50, No.3.4, 203-206, 2003. (Summary) The patient was a low birth weight infant with a history of renal failure. She was referred to our department 29 days after birth to undergo fundus examination. She experienced renal failure after undergoing a mydriatic test and needed medical treatment. Eyedrops containing phenylephrine were instilled several times and additional drops were also instilled during the fundal examination using an eyelid retractor, therefore the blood concentration of the drug was elevated sufficiently to contract the renal vessels, ultimately inducing renal failure. The present case suggests that since the use of mydriatic eyedrops in low birth weight infants could induce renal failure, the following points should be considered: 1) Mydriatic eyedrops should be used with caution by monitoring mydriasis and avoiding excessive instillation; 2) After instillation, the lacrimal region should be compressed to prevent the flow of mydriatic drops to the nasolacrimal canal; and 3) Vital signs should be monitored to check the onset of any adverse reactions for 12 hours after fundal examination. (Keyword) Renal failure / mydriatic drug / phenylephrine / retinopathy of prematurity (Tokushima University Institutional Repository) ● Metadata: 110706 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 13678392 ● CiNii @ National Institute of Informatics (CRID): 1050282812442868096 ● Search Scopus @ Elsevier (PMID): 13678392 (Tokushima University Institutional Repository: 110706, PubMed: 13678392, CiNii: 1050282812442868096)
37.	Takashi Katome, Toshiyuki Obata, Rie Matsushima, Norihisa Masuyama, Lewis C. Cantley, Yukiko Gotoh, Kazuhiro Kishi, Hiroshi Shiota and Yousuke Ebina : Use of RNA interference-mediated gene silencing and adenoviral overexpression to elucidate the roles of AKT/protein kinase B isoforms in insulin actions, The Journal of Biological Chemistry, Vol.278, No.30, 28312-28323, 2003. (Summary) Insulin plays a central role in the regulation of glucose homeostasis in part by stimulating glucose uptake and glycogen synthesis. The serine/threonine protein kinase Akt has been proposed to mediate insulin signaling in several processes. However, it is unclear whether Akt is involved in insulin-stimulated glucose uptake and which isoforms of Akt are responsible for each insulin action. We confirmed that expression of a constitutively active Akt, using an adenoviral expression vector, promoted translocation of glucose transporter 4 (GLUT4) to plasma membrane, 2-deoxyglucose (2-DG) uptake, and glycogen synthesis in both Chinese hamster ovary cells and 3T3-L1 adipocytes. Inhibition of Akt either by adenoviral expression of a dominant negative Akt or by the introduction of synthetic 21-mer short interference RNA against Akt markedly reduced insulin-stimulated GLUT4 translocation, 2-DG uptake, and glycogen synthesis. Experiments with isoform-specific short interference RNA revealed that Akt2, and Akt1 to a lesser extent, has an essential role in insulin-stimulated GLUT4 translocation and 2-DG uptake in both cell lines, whereas Akt1 and Akt2 contribute equally to insulin-stimulated glycogen synthesis. These data suggest a prerequisite role of Akt in insulin-stimulated glucose uptake and distinct functions among Akt isoforms. (Keyword) 3T3 Cells / Adenoviridae / Animals / Base Sequence / brain / CHO Cells / COS Cells / Cricetinae / Deoxyglucose / Dose-Response Relationship, Drug / Gene Library / Gene Silencing / Genetic Techniques / Glucose Transporter Type 4 / Glycogen / Immunoblotting / insulin / Luciferases / Mice / Molecular Sequence Data / Monosaccharide Transport Proteins / Muscle Proteins / Plasmids / Precipitin Tests / Protein Isoforms / Protein Transport / Protein-Serine-Threonine Kinases / Proto-Oncogene Proteins / Proto-Oncogene Proteins c-akt / RNA Interference / Rats / Time Factors (Link to Publication Site) ● Publication site (DOI): 10.1074/jbc.M302094200 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12734182 ● CiNii @ National Institute of Informatics (CRID): 1572543026620544384 ● Search Scopus @ Elsevier (PMID): 12734182 ● Search Scopus @ Elsevier (DOI): 10.1074/jbc.M302094200 (DOI: 10.1074/jbc.M302094200, PubMed: 12734182, CiNii: 1572543026620544384)
38.	高田朋子, 石丸真弓, Kayo Shinomiya, Takeshi Naito and Hiroshi Shiota : Traumatic rhegmatogenous retinal detachment due to bungee jumping, Ophthalmology, Vol.45, No.6, 947-950, 2003. (Keyword) 眼外傷 / バンジージャンプ / 外傷性網膜剥離 / eye injury / bungeejumping / traumatic retinal detachment (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520854804954893056 (CiNii: 1520854804954893056)
39.	Eiko Murata, Saimoon Sharmin, Hiroshi Shiota, Mayumi Shiota, Mihiro Yano and Hiroshi Kido : The effect of topically applied secretory leukocyte protease inhibitor on the eosinophil response in the late phase of allergic conjunctivitis, Current Eye Research, Vol.26, No.5, 271-276, 2003. (Summary) This study examined the effects of secretory leukocyte protease inhibitor (SLPI), a protease inhibitor in tears, in allergic conjunctivitis. Conjunctiva of male Hartley guinea pigs sensitized with ovalbumin were treated with SLPI or the vehicle 10 min before antigen challenge or simultaneously. The animals were sacrificed after antigen challenges of 0-24 h duration, and the inhibition of eosinophil conjunctival migration and degranulation by SLPI was analyzed histochemically. The effects of SLPI on mast cell chymase and tryptase were also examined. Treatment of sensitized guinea pigs with SLPI suppressed the conjunctival recruitment and degranulation of eosinophils after antigen challenge for 6 h, inhibiting the development of allergic conjunctivitis. The effects of SLPI were observed at concentrations > or =0.1 microM, with a peak at 5 microM. SLPI inhibited chymase in a dose-dependent manner, but had no effect on tryptase. The topical SLPI application may be therapeutic in allergic conjunctivitis. (Keyword) Administration, Topical / Animals / Cell Degranulation / Cell Movement / Chymases / Conjunctivitis, Allergic / Eosinophils / Guinea Pigs / Humans / Male / Mast Cells / Osmolar Concentration / Proteinase Inhibitory Proteins, Secretory / Proteins / Secretory Leukocyte Peptidase Inhibitor / Serine Endopeptidases / Serine Proteinase Inhibitors / Tryptases (Link to Publication Site) ● Publication site (DOI): 10.1076/ceyr.26.4.271.15429 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12854054 ● Search Scopus @ Elsevier (PMID): 12854054 ● Search Scopus @ Elsevier (DOI): 10.1076/ceyr.26.4.271.15429 (DOI: 10.1076/ceyr.26.4.271.15429, PubMed: 12854054)
40.	Fumiko Ueno, Hiroshi Shiota, Maki Miyaura, Akiko Yoshida, Akiko Sakurai, Junko Tatsuki, Hajime A Koyama, Hirofumi Akari, Akio Adachi and Mikako Fujita : Vpx and Vpr proteins of HIV-2 up-regulate the viral infectivity by a distinct mechanism in lymphocytic cells, Microbes and Infection, Vol.5, No.5, 387-395, 2003. (Summary) Mutants of human immunodeficiency virus type 2 (HIV-2) carrying a frame-shift mutation in vpx, vpr, and in both genes were monitored for their growth potentials in a newly established lymphocytic cell line, HSC-F. Worthy of note, the replication of a vpx single mutant, but not vpr, was severely impaired in these cells, and that of a vpx-vpr double mutant was more damaged. Defective replication sites of the vpx single and vpx-vpr double mutants were demonstrated to be mapped, respectively, to the nuclear import of viral genome, and to both, this process and the virus assembly/release stage. While the mutational effect of vpr was small, the replication efficiency in one cycle of the vpx mutant relative to that of wild-type virus was estimated to be 10%. The growth phenotypes of the vpx, vpr, and vpx-vpr mutant viruses in HSC-F cells were essentially repeated in primary human lymphocytes. In primary human macrophages, whereas the vpx and vpx-vpr mutants did not grow at all, the vpr mutant grew equally as well as the wild-type virus. These results strongly suggested that Vpx is critical for up-regulation of HIV-2 replication in natural target cells by enhancing the genome nuclear import, and that Vpr promotes HIV-2 replication somewhat, at least in lymphocytic cells, at a very late replication phase. (Keyword) HIV-2 / Vpx / Vpr (Link to Publication Site) ● Publication site (DOI): 10.1016/S1286-4579(03)00042-X (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12737994 ● Summary page in Scopus @ Elsevier: 2-s2.0-0037651878 (DOI: 10.1016/S1286-4579(03)00042-X, PubMed: 12737994, Elsevier: Scopus)
41.	Keiko Okamoto, Hiroshi Eguchi, Sou Hata, Maki Nishino, Miyamoto Tatsuro and Hiroshi Shiota : A Case of Suture-Related Corneal Infection Eight Years after Penetrating Keratoplasty, Folia Ophthalmologica Japonica, Vol.54, No.2, 135-138, 2003. (Keyword) 全層角膜移植術 / 縫合糸感染症 / biofilm (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1570009750061544960 ● Summary page in Scopus @ Elsevier: 2-s2.0-0038674663 (CiNii: 1570009750061544960, Elsevier: Scopus)
42.	Kyoko Fuke, Tomihiko Hirokawa, Makoto Kajima, Yoichi Mikawa, Takeshi Naito and Hiroshi Shiota : Color Doppler Imaging Test Effect on Corneal Endothelium, Journal of the Eye, Vol.20, No.1, 115-117, 2003. (Keyword) orbit / color Doppler imaging / safety / corneal endothelium (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1521417755450507520 (CiNii: 1521417755450507520)
43.	N Katunuma, A Ohashi, E Sano, E Murata, Hiroshi Shiota, K Yamamoto, E Majima and QT Le : New cysteine protease inhibitors in physiological secretory fluids and their medical significance, Advances in Enzyme Regulation, Vol.43, No.1, 393-410, 2003. (Keyword) Amino Acid Sequence / Animals / Caseins / Cathepsins / Cattle / Cystatins / Cysteine Proteinase Inhibitors / Dose-Response Relationship, Drug / Electrophoresis, Polyacrylamide Gel / Humans / Kinetics / Lactoferrin / Liver / Milk / Milk, Human / Molecular Sequence Data / Papain / Peptides / Protein Structure, Tertiary / Rats / Sequence Homology, Amino Acid / Structure-Activity Relationship / Transferrin (Link to Publication Site) ● Publication site (DOI): 10.1016/S0065-2571(02)00041-9 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12791399 ● Search Scopus @ Elsevier (PMID): 12791399 ● Search Scopus @ Elsevier (DOI): 10.1016/S0065-2571(02)00041-9 (DOI: 10.1016/S0065-2571(02)00041-9, PubMed: 12791399)
44.	Tatsuro Miyamoto, Hiroshi Eguchi, Tomoko Tkata, Sou Hata, Kayo Shinomiya and Hiroshi Shiota : Two Cases of Therapeutic Penetrating Keratoplasty using Fresh Donor Cornea for Non-traumatic Corneal Perforation, Journal of the Eye, Vol.19, No.11, 1481-1484, 2002. (Keyword) non-traumatic corneal perforation / fresh donor cornea / therapeutic penetrating keratoplasty / stage of procedure (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1522262180370574976 (CiNii: 1522262180370574976)
45.	Hiroshi Eguchi, Iwata Akiko, Sogabe Maki, Sato Hiroyuki and Hiroshi Shiota : 光干渉断層計を前眼部に応用した続発緑内障の1例, Acta Societatis Ophthalmologicae Japonicae, Vol.106, No.3, 178-181, 2002. (Keyword) 光干渉断層計 / 前眼部 / 続発緑内障 / 全層角膜移植術後 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1570291224723546880 (CiNii: 1570291224723546880)
46.	Tomihiko Hirokawa, Tomoko Nishikage, Takehiro Moroe, Makoto Kajima, Masakazu Hayashi, Takeshi Naito, Shinta Yamane and Hiroshi Shiota : Visualization of Uveal Perfusion by Contrast-Enhanced Harmonic Ultrasonography at a Low Mechanical Index, --- A Pilot Animal Study ---, Journal of Ultrasound in Medicine, Vol.21, No.3, 299-307, 2002. (Keyword) contrast-enhanced ultrasonography / orbital blood perfusion / low mechanical index / harmonic imaging (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0036198330 (Elsevier: Scopus)
47.	Yasuhisa Sakaguchi, Hiroshi Eguchi, Sou Hata, Takeshi Naito and Hiroshi Shiota : Two pediatric cases of perforationg globe injury developing different prognosis, Ophthalmology, Vol.44, No.5, 211-214, 2002. (Keyword) 穿孔性眼外傷 / 小児 / 硝子体手術 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1521136280386036736 (CiNii: 1521136280386036736)
48.	Maki Nishino, Hiroshi Eguchi, Yuko Terada, Tatsuro Miyamoto and Hiroshi Shiota : 徳島大学における角膜移植術の統計的観察, Journal of the Eye, Vol.19, 1485-1488, 2002. (Keyword) 角膜移植 / statistics / ドナー側因子 / 生存分析
49.	Akiko Iwata, Hiroshi Eguchi, Hiroshi Shiota, 高橋昭良, Koji Yasutomo, 石橋広樹 and 嵩原裕夫 : 視力低下を契機に発見された小児褐色細胞腫の1例, Japanese Journal of Clinical Ophthalmology, Vol.56, No.0, 1553-1556, 2002. (Keyword) 視力低下 / infant / 高血圧眼底 / 褐色細胞腫 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1570291225909602944 ● Summary page in Scopus @ Elsevier: 2-s2.0-0036445279 (CiNii: 1570291225909602944, Elsevier: Scopus)
50.	Maki Sogabe, Masakazu Hayashi, 賀島誠, Takeshi Naito and Hiroshi Shiota : Submacular surgery for macular hard exudates in two non-diabetic patients, Japanese Journal of Clinical Ophthalmology, Vol.55, No.11, 1785-1788, 2001. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390565159895592576 ● Summary page in Scopus @ Elsevier: 2-s2.0-0034757841 (CiNii: 1390565159895592576, Elsevier: Scopus)
51.	Tomihiko Hirokawa, Takeshi Naito, Hiroshi Shiota, Hajime Nunomura, Jun Minakuchi, Masahiro Kyo and Kohta Takahashi : Cytomegalovirus retinitis in a case with chronic rejetion after kidney transplantation, Japanese Journal of Clinical Ophthalmology, Vol.55, No.9, 1669-1672, 2001. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390002209942771584 ● Summary page in Scopus @ Elsevier: 2-s2.0-0034789343 (CiNii: 1390002209942771584, Elsevier: Scopus)
52.	Akiko Iwata, Hiroshi Eguchi, Hiroyuki Sato, Kayo Shinomiya and Hiroshi Shiota : Penetrating Keratoplasty Using Intentional Double Anterior Chamber, Journal of the Eye, Vol.18, No.8, 1045-1047, 2001. (Keyword) 意図的二重前房 / 全層角膜移植術 / 長期予後 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1572543025108484224 (CiNii: 1572543025108484224)
53.	Hiroshi Eguchi, Hiroyuki Sato, Makoto Kajima, Hiroshi Shiota and Youichi Mikawa : A case of corneal amyloidosis secondary to entropion, Japanese Journal of Clinical Ophthalmology, Vol.55, No.6, 1123-1126, 2001. (Keyword) 角膜アミロイドーシス / 睫毛内反症 / 角膜表層切除術 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390565159895612032 ● Summary page in Scopus @ Elsevier: 2-s2.0-0034924815 (CiNii: 1390565159895612032, Elsevier: Scopus)
54.	Tomihiko Hirokawa, Takeo Ohki, Yuki Hayashi, Masakazu Hayashi, Takeshi Naito and Hiroshi Shiota : Effects of Latanoprost and Unoprostone on Blood Flow Velocity of the Retrobulbaar Vessels in Rabbits, Journal of the Eye, Vol.18, No.5, 659-663, 2001. (Keyword) ラタノプロスト / ウノプロストン / 超音波カラードプラ法 / 眼循環 / latanoprost / unoprostone / ultrasound color Doppler imaging / ocular circulation (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1572261550131344000 (CiNii: 1572261550131344000)
55.	Masakazu Hayashi, Hiroko Tajika, Takeshi Naito and Hiroshi Shiota : A Case of Proliferative Diabetic Retinopathy with Increasing Blood Glucose Level After Subconjunctival Corticosteroid Injection, Folia Ophthalmologica Japonica, Vol.52, No.2, 147-149, 2001. (Keyword) ステロイド結膜下注射 / ステロイド糖尿病 / 増殖糖尿病網膜症 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1570291224776228480 (CiNii: 1570291224776228480)
56.	Takashi Asahara, Kayo Shinomiya, Takeshi Naito and Hiroshi Shiota : Induction of Gene into the Rabbit Eye by Iontophoresis:Preliminary Report, Japanese Journal of Ophthalmology, Vol.45, No.1, 31-39, 2001. (Summary) To assess the transfer of 6-carboxyfluorescein (6-FAM)-labeled phosphorothioate oligonucleotides(S-ODNs) into the ocular tissues, their stability, and possibility of injury to the ocular tissues. The S-ODNs(2 mL/eye)were transduced noninvasively into albino rabbit eyes.The iontophoresis group consisted of 6 rabbits (12 eyes); the control group consisted of 2 rabbits (4 eyes) given eye drops containing S-ODNs. Aqueous humor and vitreous humor were collected after iontophoresis, subjected to electrophoresis with a fluorescence DNA sequencer and analyzed by the Gene Scan program. Frozen sections, 10-microm thick, were prepared for observation under a fluorescence microscope. A plasmid 4.7 kbp in size that expresses green fluorescent protein (GFP) was induced into the 18 eyes of 9 rabbits by the same procedure. In the iontophoresis group, S-ODNs were detected in the anterior chamber 5 minutes after electrophoresis began and in the vitreous after 10 minutes. These S-ODNs maintained the same length as at the initial synthesis. The S-ODNs could also be detected in the posterior retina 20 minutes after electrophoresis. No evidence of degeneration or inflammation due to the above procedure was found in the ocular tissues. Fluorescence showing GFP gene expression was found in the cornea, the anterior chamber angle, and the ciliary subepithelial tissues. These findings show that iontophoresis is an effective method to induce genes into the rabbit eye. (Keyword) Gene transduction / green fluorescent protein / iontophoresis / phosphorothioatc oligonucleotide (Link to Publication Site) ● Publication site (DOI): 10.1016/S0021-5155(00)00291-4 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11163043 ● Summary page in Scopus @ Elsevier: 2-s2.0-0035191885 (DOI: 10.1016/S0021-5155(00)00291-4, PubMed: 11163043, Elsevier: Scopus)
57.	Tomihiko Hirokawa, 林正和, 賀島誠, Takeshi Naito, Hiroshi Shiota and Seiya Itotagawa : Ultrasound Color Doppler Imaging Showed the Change in Orbital Hemodynamics in a Case of Anterior Ischemic Optic Neuropathy, 第66回日本中部眼科学会, 1050-1053, 2001. (Keyword) Anterior Ischemic Optic Neuropathy(AION) / Color Doppler Imaging
58.	Hiroyuki Sato, Hiroshi Eguchi, Makoto Kajima, Takeshi Naito and Hiroshi Shiota : 再燃を示唆する所見として視神経浸潤を認めた緩解期AMLの1例, Ophthalmology, Vol.43, 1207-1210, 2001. (Keyword) 視神経浸潤 / AML / 再燃
59.	Hiroko Endo, Takeshi Naito, Takashi Asahara and Hiroshi Shiota : Cytokines in the Vitreous Fluid of Patients with Proliferative Diabetic Retinopathy, --- Vascular Endothelial Growth Factor and Platlet-derived Growth Factor Are Elevated in Proliferative Diabetic Retinopathy ---, Acta Societatis Ophthalmologicae Japonicae, Vol.104, No.10, 711-716, 2000. (Keyword) Proliferative diabetic retinopathy(PD-R) / Vascular endothelial growth factor(VEGF) / Platlet-derived growth factor(PDGF) / Protein concentration in the aqueous humor
60.	Tomohiro Ise, Hiroshi Eguchi, Aya Kagawa, Makoto Kajima, Nan Fu and Hiroshi Shiota : 角膜真菌症9例の検討, Ophthalmology, Vol.42, 807-910, 2000. (Keyword) 角膜真菌症
61.	Hiroshi Eguchi, Maki Sogabe, Hiroshi Shiota, Kubo Takayuki and Keisuke Izumi : 高周波メスを併用した角膜上皮形成術, Journal of the Eye, Vol.17, No.11, 1551-1553, 2000. (Keyword) 高周波メス / Stevens-Johnson 症候群 / 角膜上皮形成術
62.	Takashi Asahara, Kouji Fukumoto, Takeshi Naito and Hiroshi Shiota : Results of submacular surgery for age-related macular degeneration, gankarinsyoiho, Vol.93, No.8, 1237-1240, 1999.
63.	Mariko Takahashi, Naozumi Ishimaru, Kumiko Yanagi, Kaoru Saegusa, Norio Haneji, Hiroshi Shiota and Yoshio Hayashi : Requirement for splenic CD4+ T cells in the immune privilege of the anterior chamber of the eye, Clinical and Experimental Immunology, Vol.116, No.2, 231-237, 1999. (Keyword) ACAID / Tolerrance / DTH / CD4+ T cells / Cytokine mRNA
64.	浅原貴志, Kayo Shinomiya, Takeshi Naito and Hiroshi Shiota : Induction of Genes into the Rabbit Eye by Iontophoresis, Acta Societatis Ophthalmologicae Japonicae, Vol.103, No.3, 178-185, 1999. (Summary) After inducing 6-carboxyfluorescein (6-FAM)-labeled phosphorothioate oligonucleotides (S-ODNs) noninvasively into albino rabbit eyes by iontophoresis, we assessed the transfer of S-ODNs into the ocular tissues, their stability, and the possible presence of injury to the ocular tissues. The iontophoresis group consisted of 12 eyes of 6 rabbits and the control group consisted of 4 eyes of 2 rabbits given eye drops containing S-ODNs. Aqueous humor and vitreous humor were collected after iontophoresis, subjected to electrophoresis with a fluorescent DNA sequencer and analyzed by the Gene Scan program. Frozen sections at 10 microns were prepared for observations under a fluorescent microscope. A plasmid 4.7 kbp in size that expresses green fluorescent protein (GFP) was induced into 18 eyes of 9 rabbits by the same procedure. In the iontophoresis group, S-ODNs were detected in the anterior chamber 5 minutes after electrophoresis and in the vitreous 10 minutes after. These S-ODNs maintained the same length as at the initial synthesis. S-ODNs could also be detected in the posterior retina 20 minutes after electrophoresis. No evidence of degeneration or inflammation due to the above procedure was found in the ocular tissues. Fluorescence showing GFP gene expressions were found in the cornea, the anterior chamber angle, and the ciliary subepithelial tissues. These findings show that iontophoresis is an effective method to induce gene into rabbit eyes. (Keyword) Iontophoresis / Phosphorothioate oligonucleotide / Green fluorescent protein (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10214051 ● CiNii @ National Institute of Informatics (CRID): 1574231874309089920 ● Summary page in Scopus @ Elsevier: 2-s2.0-0032976976 (PubMed: 10214051, CiNii: 1574231874309089920, Elsevier: Scopus)
65.	Takeshi Naito, Takashi Asahara, Makoto Kajima and Hiroshi Shiota : Outcome of submacular surgery for diabetic macular exudates, Japanese Journal of Clinical Ophthalmology, Vol.53, No.1, 69-72, 1999.
66.	Hiroshi Eguchi, Takeshi Naito, Hiroshi Shiota and Shuuichi Miyazaki : 視神経乳頭陥凹拡大での血清リポタンパク(a)値の検討, Folia Ophthalmologica Japonica, Vol.50, 410-412, 1999. (Keyword) 血清Lp(a)値 / 視神経乳頭陥凹拡大
67.	Hiroshi Eguchi, Yasuo Kamada, Tomoko Kanagawa, Takeshi Naito and Hiroshi Shiota : フザリウムによる角膜真菌症, Japanese Journal of Clinical Ophthalmology, Vol.53, 609-611, 1999. (Keyword) フザリウム / 角膜真菌症
68.	Eiji Kudo, Hiroshi Shiota, Takeshi Naito, K Satake and Mitsuo Itakura : Polymorphisms of Thymidine Kinase Gene in Herpes Simplex Virus Type 1, --- Analysis of Clinical Isolates From Herpetic Keratitis Patients and Laboratory Strains ---, Journal of Medical Virology, Vol.56, No.2, 151-158, 1998. (Summary) Drug-resistance of herpes simplex virus (HSV) is caused most frequently by mutation of the viral thymidine kinase (TK) gene. To elucidate the significance of detecting nucleotide changes of the TK gene for screening drug-resistant viruses, the frequency and variation of the genetic polymorphisms in the whole coding region of the TK gene were studied in 14 acyclovir-susceptible HSV type 1 (HSV-1) clinical isolates from 14 patients with epithelial herpetic keratitis. Two reference HSV-1 laboratory strains, McKrae and PH, and two acyclovir-resistant variants of the PH strain were also studied as controls. Polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) and direct sequencing detected nucleotide differences at 24 positions, and amino acid substitutions at 12 codons in the TK gene of the examined viruses. Nucleotide diversity of 0.0029 per base (the average number of nucleotide substitutions of 3.3 per 1,131 base pairs) in the TK gene in the clinical isolates was comparable to 0.0037 per base of the whole HSV-1 genome in Japanese isolates reported previously. PCR-SSCP analysis of the acyclovir-resistant strains easily detected aberrantly shifted bands by comparing them with those of the parental strain, followed by the quick determination of mutated sequences. These results suggest that detection of nucleotide changes of the TK gene is useful for serial observation of persistent or recurrent HSV infection as observed in immunocompromised hosts, but that it is not useful for screening drug-resistant viruses from nonepidemic clinical isolates because of the comparable genetic polymorphisms in the TK gene as in the whole HSV-1 genome. (Keyword) HSV-1 isolates / PCR-SSCP / direct sequencing / genetic polymorphism / acyclovir resistance (Link to Publication Site) ● Publication site (DOI): 10.1002/(SICI)1096-9071(199810)56:2<151::AID-JMV9>3.0.CO;2-7 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9746072 ● Summary page in Scopus @ Elsevier: 2-s2.0-0031656502 (DOI: 10.1002/(SICI)1096-9071(199810)56:2<151::AID-JMV9>3.0.CO;2-7, PubMed: 9746072, Elsevier: Scopus)
69.	Kayo Shinomiya, Sayaka Yamanaka, Koji Fukumoto, Hiroshi Shiota and Kenichi Hirai : A Case of Herpetic Keratitis Associated With Keratomycosis, Journal of the Eye, Vol.15, No.1, 117-119, 1998. (Keyword) herpetic keratitis / keratomycosis / Fusarium solani (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1574231875111553024 (CiNii: 1574231875111553024)
70.	Hiroko Suenaga, 遠藤弘子, 浅原貴志 and Hiroshi Shiota : 全層角膜移植術後の遷延性角膜上皮欠損, Folia Ophthalmologica Japonica, Vol.49, 844-847, 1998.
71.	Hiroko Endo, Takeshi Naito, Takashi Asahara, Makoto Kajima and Hiroshi Shiota : Results of Vitrectomy for Proliferative Diabetic Retinopathy at Department of Ophthalmology,the University of Tokushima, Folia Ophthalmologica Japonica, Vol.49, No.10, 830-833, 1998. (Keyword) Proliferative Diabetic Retinopathy(PD-R) / Vitrectomy / Macular Detachmant
72.	Takeshi Naito, Kayo Miyoshi, Hideki Tani, Keiko Satake and Hiroshi Shiota : Surgical outcome of idiopathic macular holes, Japanese Journal of Clinical Ophthalmology, Vol.51, No.4, 694-696, 1997.
73.	Hideki Tani, Takeshi Naito, Hiroshi Shiota and Yasuo Mimura : A Study of Acute Infection of Herpes Simplex Virus in Mouse Trigeminal Ganglia, Acta Societatis Ophthalmologicae Japonicae, Vol.100, No.7, 496-500, 1996. (Keyword) HSV-1 / Trigeminal ganglion / CD4+ cells / CD8+ cells
74.	Hideki Tani, Takeshi Naito, Hiroshi Shiota and Yasuo Mimura : Outcome of surgery for idiopathic epiretinal membrane, Japanese Journal of Clinical Ophthalmology, Vol.50, No.6, 1175-1178, 1996.
75.	Hiroshi Shiota, Keiko Nitta, Takeshi Naito, Yasuo Mimura and Tokumi Maruyama : Clinical evaluation of carbocyclic oxetanocin G eyedrops in the treatment of herpes simplex corneal ulcers, The British journal of Ophthalmology, Vol.80, No.5, 413-415, 1996. (Summary) Acyclovir (ACV) ophthalmic ointment is effective in the treatment of herpetic keratitis. However, when applied, the ointment has an unpleasant feeling and some cases are resistant to ACV. A new antiviral compound, carbocyclic oxetanocin G (C.OXT-G) has potent anti-herpes simplex virus activity and high water solubility, so the clinical effect of C.OXT-G eyedrops on ulcerative herpetic keratitis was evaluated. Studies were conducted on the corneal ulcers in 37 eyes of 27 patients. Patients with typical dendritic or geographic corneal ulcers were treated with 0.1% C.OXT-G eyedrops, applied five times a day, together with eyedrops of an antibiotic applied four times a day. The eyes were examined at least twice a week until the ulcers healed, and thereafter at intervals for up to 3 months. All of the ulcers healed, their average healing time being 4.9 (SD 2.2) (range 2 to 9) days. The ulcers in 20 of the 37 eyes were induced by the use of corticosteroid or immunosuppressive drugs, and their average healing time was 4.8 (2.3) days. No adverse drug reactions were seen during the observation period in this trial. Eyedrops containing 0.1% C.OXT-G are excellent and safe for treatment of herpes simplex corneal ulcers in humans. (Keyword) Administration, Topical / Anti-Infective Agents / Antiviral Agents / Corneal Ulcer / Eye Infections, Viral / Female / Guanine / Humans / Keratitis, Herpetic / Male / Middle Aged / Ofloxacin / Ophthalmic Solutions (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 8695561 ● Summary page in Scopus @ Elsevier: 2-s2.0-0029996853 (PubMed: 8695561, Elsevier: Scopus)
76.	Keiko Nitta, Takeshi Naito, Hiroshi Shiota and Yasuo Mimura : Surgical outcome of macular pucker in our institution, gankarinsyoiho, Vol.90, No.2, 122-124, 1996.
77.	Keiko Nitta, Hiroshi Shiota, Takeshi Naito, Yasuo Mimura and Tokumi Maruyama : The Antiherpetic Effect of 9-(2-Deoxy-2-fluoro-β-D-arabinofuranosyl)adenine, Journal of the Eye, Vol.13, No.1, 91-92, 1996. (Keyword) 9-(2-Deoxy-2-fluoro-β-D-arabinofuranosyl)adenine / antiherpetic activity / aciclovir / aciclovir-resistant HSV
78.	Keiko Nitta, Takeshi Naito, Hiroshi Shiota and Yasuo Mimura : Surgical Outcomes in Rhegmatogenous Retinal Detachment during the Past Five Years at Our Institution, Japanese Journal of Ophthalmic Surgery, Vol.8, No.1, 135-137, 1995. (Keyword) rhegmatogenous retinal detachment / surgical procedure / reattachment rate / postoperative visual acuity / bilateral retinal detachment (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1573387449559728512 (CiNii: 1573387449559728512)
79.	Keiko Nitta, Hiroshi Shiota, Takeshi Naito and Yasuo Mimura : Sensitivities to Other Antiviral Drugs and Thymidine Kinase Activity of Aciclovir-resistant Herpes Simplex Virus Type1, Acta Societatis Ophthalmologicae Japonicae, Vol.98, No.6, 513-519, 1994. (Keyword) Herpes simplex virus type1 / Aciclovirresistant strain / Antiviral drugs / (S)-HPMPA / Thymidine kinase
80.	Takeshi Naito, Keiko Nitta, Hiroshi Shiota and Yasuo Mimura : A Case of Ocular Methicillin resistant Staphylococcus aureus Infection after Cataract Surgery, Journal of the Eye, Vol.10, No.11, 1877-1880, 1993. (Keyword) MRSA(methicillin-resistant Staphylococcus aureus) / ocular infection / cataract surgery / postoperative endophthalmitis / drug sensitivity
81.	Takeshi Naito, Keiko Nitta, Yasuhito Kinouchi, Hiroshi Shiota and Yasuo Mimura : Fate of fellow eye after retinal detachment surgery, Japanese Journal of Clinical Ophthalmology, Vol.45, No.3, 347-349, 1991.
82.	Takeshi Naito, Keiko Nitta, Yoshihito Kinouchi, Hiroshi Shiota and Yasuo Mimura : Effects of 9-(1,3-dihydroxy-2-propoxymethyl)guanine(DHPG)eye drops and cyclosporine eye drops in the treatment of herpetic stromal keratitis in rabbits, Current Eye Research, Vol.10, 201-203, 1991.
83.	Takeshi Naito, Keiko Nitta, Yasuhito Kinouchi, Hiroshi Shiota and Yasuo Mimura : Studies on Herpes Zoster Ophthalmicus, Journal of the Eye, Vol.7, No.9, 1359-1361, 1990. (Keyword) varicella-zoster virus / herpes zoster ophthalmicus / ocular complications
84.	Takeshi Naito, Hiroshi Shiota, Keiko Nitta and Yasuo Mimura : Effects on herpes simplex virus type 1 of combined use of interferon-beta and anti-herpetic agents in vitro, Japanese Journal of Ophthalmology, Vol.34, No.1, 15-21, 1990. (Summary) It has been shown that the use of interferon (IFN) alone has little or no therapeutic effect in the treatment of herpetic keratitis. However, IFN used in combination with anti-herpetic agents has high therapeutic efficacy. This interaction between IFN and anti-herpetic agents has not been elucidated clearly. In this study, the interaction between human fibroblast interferon (HuIFN-beta) and anti-herpetic agents, 5-iodo-2'-deoxyuridine (IDU), aciclovir (ACV) and 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG), was studied in vitro using VERO cells. After the formation of a monolayer of VERO cells, HuIFN-beta of various concentrations was added to the maintenance medium. After 24 hours incubation, the medium was removed and herpes simplex virus (HSV) type 1 was inoculated on the VERO cells. Then, anti-herpetic agents were added to the medium in various concentrations. After 48 hours incubation, plaques were counted and the reduction rate in numbers of plaque was calculated. It was found that the combination of HuIFN-beta with IDU, ACV or DHPG worked synergistically in the inhibition of HSV replication. (Keyword) Anti-herpetic agents / herpetic keratitis / interferon synergism (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 2163462 ● Summary page in Scopus @ Elsevier: 2-s2.0-0025274735 (PubMed: 2163462, Elsevier: Scopus)
85.	Takeshi Naito, Hiroshi Shiota, Keiko Nitta, Kouhei Kusujima and Yasuo Mimura : Studies on the Efficacy of 9-(1,3-Dihydroxy-2-propoxymethyl)Guanine(DHPG)Eye Drops Against Herpetic Keratitis in Rabbits, Journal of the Eye, Vol.6, No.1, 122-125, 1989. (Keyword) DHPG eye drops / herpetic keratitis / interferon / synergism
86.	Hiroshi Shiota, Takeshi Naito, 楠島康平, 加藤俊彦 and 三村康男 : 角膜ヘルペスの治療に対するインターフェロンの応用, Japanese Journal of Clinical Ophthalmology, Vol.42, No.5, 542-543, 1988.
87.	Takeshi Naito, Hiroshi Shiota, Seiji Kanematsu, Yasuo Mimura and Takashi Ogawa : Studies on the Cytotoxicity of a New Anti-herpetic Agent,9-(1,3-dihydroxy-2-propoxymethyl)guanine,on the Rabbit Cornea, Acta Societatis Ophthalmologicae Japonicae, Vol.92, No.4, 573-577, 1988. (Keyword) DHPG / Anti-herpetic agent / Cytotoxicity / Scanning electron microscopy / Herpetic keratitis
88.	Keiko Nitta, Takeshi Naito, Hiroshi Shiota and Yasuo Mimura : Clinical Evaluation of Aciclovir against Herpetic Keratitis, Folia Ophthalmologica Japonica, Vol.38, No.1, 78-82, 1987.
89.	Hiroshi Shiota, Takeshi Naito and Yasuo Mimura : Anti-herpes simplex virus(HSV)effect of 9-(1,3-dihydroxy-2-propoxymethyl)guanine, Current Eye Research, Vol.6, No.1, 241-245, 1987.
90.	Takeshi Naito, Hiroshi Shiota and Yasuo Mimura : Side effects in the treatment of herpetic keratitis, Current Eye Research, Vol.6, No.1, 237-239, 1987.
91.	Takeshi Naito, Hiroshi Shiota, Keiko Nitta and Yasuo Mimura : Various Side Effects in the Treatment of Herpetic Keratitis, Folia Ophthalmologica Japonica, Vol.37, No.6, 849-852, 1986.

Academic Paper (Unrefereed Paper):

1.	西岡麻未子, 梅原麻子, Toshiyuki Obata, Hiroshi Shiota, Yousuke Ebina and Seiichi Hashida : Development of the Diabetic Risk Evaluation using Resistin and Insulin Receptor-alpha in Urine and the Application to Exercise/Nutrition Education, Research bulletin of Tokushima Bunri University, Vol.76, 1-19, 2008. (Summary) Aim: We want to be able to detect diabetic risky subjects early, and to prevent the onset of diabetes mellitus by performing exercise guidance and offering nutritional education to them. Methods: Two ultrasensitve immunoassays (immune complex transfer enzyme immunoassay; ICT-EIA) for resistin and soluble insulin receptor-a (sIRα) were developed. Urine samples from the patients of diabetes mellitus (DM; n=70), the non-diabetes obese subjects (NDMO; n=43) and the non-diabetes non-obese subjects (NDMNO; n=78) were measured resistin and sIRα using ICT-EIAs. Results: Detection limits of ICT-EIA for resistin and sIRa were 2pg/ml and 0.05pg/ml, respectively. It is possible to measure resistin and sIRa in the urine directly. Resistin in the urine from DM, NDMO and NDMNO were 30.0±36.2, 15.4±17.2 and 17.8±17.4 ng/mg of creatinine, respectively. The urine concentrations of resistin in DM were significantly higher than in the non-diabetes subjects (NDMO and NDMNO) (P<0.05). sIRa in the urine from DM, NDMO and NDMNO were 11.4±21.8, 1.9±1.1 and 1.3±0.8 pg/mg of creatinine, respectively. The urine concentrations of sIRa in DM were significantly higher than in NDM and NDMNO (P<0.001 and P<0.001), respectively. Furthermore, resistin in the urine was positively correlated to sIRa, in the urine. Conclusion: Both resistin and sIRoc in the urine are good markers for the risk of diabetes mellitus. As a result, these markers will be effective for the evaluation of exercise guidance and nutritional education. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520853834078437888 (CiNii: 1520853834078437888)
2.	Hiroshi Shiota : なぜ今，院内感染の予防対策が必要なのか?, NANO OPHTHALMOLOGY, No.33, 3, 2007.
3.	Fumiko Murao, 西野真紀, Makoto Kajima, Takeshi Naito and Hiroshi Shiota : Outcome of primary vitrectomy for proliferative diabetic retinopathy with rubeosis in the iris and chamber angle, Japanese Journal of Clinical Ophthalmology, Vol.59, No.9, 1535-1538, 2005. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1521417754764176512 ● Summary page in Scopus @ Elsevier: 2-s2.0-26644466294 (CiNii: 1521417754764176512, Elsevier: Scopus)
4.	長澤利彦, Takeshi Naito, Makoto Kajima and Hiroshi Shiota : Outcome of vitreous surgery for branch retinal vein occlusion, Japanese Journal of Clinical Ophthalmology, Vol.59, No.8, 1325-1328, 2005. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1523106605573803776 ● Summary page in Scopus @ Elsevier: 2-s2.0-24644516667 (CiNii: 1523106605573803776, Elsevier: Scopus)
5.	Eiji Kudo and Hiroshi Shiota : 糖尿病性細小血管症, Nihon Rinsho. Japanese Journal of Clinical Medicine, Vol.63, No.6, 302-305, 2005. (Summary) 各論 Ⅰ．糖尿病網膜症特論遺伝子治療としてのRNAi (RNAi as a gene therapy approach) (Keyword) RNAi / gene therapy / diabetic retinopathy
6.	片山智子, 齋藤晴比古, Hiroshi Shiota and 勝沼信彦 : 涙液中の特殊蛋白検出によるベーチェット病，原田病の診断, 逓信医学, Vol.57, No.3, 203-204, 2005.
7.	Khadka B. Kamal, Takeshi Naito, Makoto Kajima, Hiroshi Shiota, Junsuke Akura and Takayuki Kiryuu : Eye care project in Gaur, Nepal, The Journal of Medical Investigation : JMI, Vol.51, No.3,4, 230-233, 2004. (Summary) The aim of the eye care project is to clear the backlog of cataract blindness for the people of Gaur and its surrounding districts in southeast Nepal. The purpose of this study is to analyze the progress of this eye care project. In mid 1997, the Nepal Netra Jyoti Sangh (NNJS), the national society for comprehensive eye care in Nepal, with financial support from the Association for Ophthalmic Cooperation to Asia (AOCA) and the 24 Hour Television (24HTV) Charity Committee established an eye hospital in the district headquarters of Gaur under the name of Narayani Eye Care Project (NECP). The hospital provides surgery, an outpatient department (OPD) and emergency services. We analyzed the number of patients, the number of surgeries and the profile of patients. The hospital was found to be providing services with modern equipment and instruments and the number of patients frequenting it had increased in accordance with the improved services. From mid 1997 until the end of 2002, the hospital provided services to a total of 122,093 patients and performed 6,143 major surgeries and 541 minor surgeries. It has been possible to conduct sight restorations of poor and blind individuals in this region at the Gaur eye hospital with the support of the NECP. (Keyword) Adolescent / Adult / Aged / Aged, 80 and over / Cataract Extraction / children / Child, Preschool / Delivery of Health Care / Female / Humans / infant / Infant, Newborn / Male / Middle Aged / Nepal (Tokushima University Institutional Repository) ● Metadata: 110746 (Link to Publication Site) ● Publication site (DOI): 10.2152/jmi.51.230 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15460911 ● Search Scopus @ Elsevier (PMID): 15460911 ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.51.230 (Tokushima University Institutional Repository: 110746, DOI: 10.2152/jmi.51.230, PubMed: 15460911)
8.	Nan Hu and Hiroshi Shiota : Emergence of resistance to carbocyclic oxetanocin G in herpes simplex virus type 1 and genetic analysis of resistant mutants, Acta Pharmacologica Sinica, Vol.25, No.7, 921-926, 2004. (Summary) To elucidate the potentiality of emergence of drug-resistance to carbocyclic oxetanocin G (C.OXT-G), a new effective antiviral drug for herpetic keratitis during treatment and the mechanism of this drug resistance. A C.OXT-G resistant strain (C.OXT-Gr) was established by serially propagating the herpes simplex virus (HSV) -1 in African green monkey kidney (VERO) cells in the presence of C.OXT-G. After the drug sensitivity assay and the thymidine kinase (TK) activity assay, the molecular basis for the drug resistance was studied using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis and PCR direct sequencing technology. After the 10th passage in 10 microm C.OXT-G, the ED50 of the C.OXT-Gr was 17.08-fold greater than that of the original strain on the average and the TK activities of these resistant strains were extremely reduced. PCR-SSCP analysis on TK gene of the wild HSV-1 and the C.OXT-Gr showed altered migration patterns in part 3 and part 4, while PCR-SSCP analysis on DNA polymerase gene showed no difference among the viruses. Sequence analysis revealed a deletion of G at position of 430 that caused frameshift, resulting in premature termination in the TK gene. The drug resistance to C.OXT-G may appear during the treatment due to the deficiency of TK activity caused by a single mutation in the TK gene of HSV-1. (Keyword) Animals / Antiviral Agents / Cercopithecus aethiops / DNA, Viral / DNA-Directed DNA Polymerase / Drug Resistance, Viral / Gene Deletion / Guanine / Herpesvirus 1, Human / Mutation / Polymerase Chain Reaction / Polymorphism, Single-Stranded Conformational / Thymidine Kinase / Vero Cells (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15210066 ● Search Scopus @ Elsevier (PMID): 15210066 (PubMed: 15210066)
9.	Shingo Matsushita, Takeshi Naito, Makoto Kajima and Hiroshi Shiota : Removal of internal limiting membrane during vitreous surgery for diabetic macular edema, Japanese Journal of Clinical Ophthalmology, Vol.58, No.6, 1029-1032, 2004. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390002209941656960 ● Summary page in Scopus @ Elsevier: 2-s2.0-3142550639 (CiNii: 1390002209941656960, Elsevier: Scopus)
10.	井上昌幸, Kayo Shinomiya, Takeshi Naito and Hiroshi Shiota : Review of a nosocomical outbreak of epidemic keratoconjuntivitis, Japanese Journal of Clinical Ophthalmology, Vol.58, No.5, 823-826, 2004. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390002209942062464 ● Summary page in Scopus @ Elsevier: 2-s2.0-3042554987 (CiNii: 1390002209942062464, Elsevier: Scopus)
11.	伊勢知弘 and Hiroshi Shiota : クラミジア結膜炎, Japanese Journal of Clinical Ophthalmology, Vol.57, No.11, 137-140, 2003. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1573105975677204736 (CiNii: 1573105975677204736)
12.	井上禎子, 斬雷 and Hiroshi Shiota : Keratomycosis, Journal of the Eye, Vol.19, No.8, 999(31)-1003(35), 2002. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1572261550106759680 (CiNii: 1572261550106759680)
13.	賀島誠, Takeshi Naito and Hiroshi Shiota : 糖尿病網膜症の治療, Shikoku Acta Medica, Vol.58, No.3, 134-138, 2002. (Tokushima University Institutional Repository) ● Metadata: 110540 (Tokushima University Institutional Repository: 110540)
14.	Hiroshi Shiota, Hiroshi Eguchi, Hiroyuki Yunoki and Shigekazu Aoyama : CAN WE IMPLANT OUR KERATOPROSTHESIS FOR HUMANS AS A TEMPORARY USE?, ANALES DEL INSTITUTO BARRAQUER, Vol.31, No.2, 199, 2002.
15.	Hiroshi Shiota, Hiroshi Eguchi, Hiroyuki Yunoki and H. Amitani : EXPERIMENTAL EVALUATION OF OUR CERAMIC KERATOPROSTHESIS IN RABBITS, ANALES DEL INSTITUTO BARRAQUER, Vol.30, 181-182, 2001.
16.	Hiroshi Shiota : 3．眼感染症の病態研究と治療法の発達, 20世紀における眼科学の総括, 51-53, 1988.

Review, Commentary:

1.	Kayo Shinomiya and Hiroshi Shiota : 【薬物選択の論点】抗ウイルス薬, Ophthalmology, Vol.48, No.2, 213-221, 2006.
2.	Kayo Shinomiya and Hiroshi Shiota : 【中毒と眼】各種内服薬と眼の副作用, Ophthalmology, Vol.47, No.2, 167-173, 2005. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1572543025721799424 (CiNii: 1572543025721799424)
3.	Nobuhiko Katunuma, Hiroshi Shiota and Trong Quang Le : Medical significance of cysteine protease inhibitors in mammalian secretory fluids, The Journal of Medical Investigation : JMI, Vol.50, No.3-4, 154-161, Aug. 2003. (Summary) New cysteine protease inhibitors in human tears and milk and their medical significance are reviewed in this paper. As protective components against bacterial infection in the eyes, we detected four kinds of anti-bacterial proteins in normal human tears including lysozyme and three kinds of cysteine protease inhibitors. Using our reverse zymography of normal tears, three kinds of cysteine protease inhibitors were found to be 78kDa, 20kDa and 15kDa and were determined to be lactoferrin, Von Ebner's Gland (VEG) protein and cystatin S, respectively. All of them belong to the cystatin super family and VEG protein and cystatin S are well known cysteine protease inhibitors. The C-terminus area 17mer peptide, Y679-K695, of lactoferrin showed strong homology with a common active domain of the cystatin family and the synthesized peptide showed inhibition of cysteine proteases. Not only were disease-specific changes found in these inhibitor profiles, but also disease-specific new inhibitors in patients tears with certain autoimmune diseases. A 35kDa inhibitor, which was detected specifically in tears with Behcet's disease, an typical autoimmune disease, was determined to be a lacrimal acidic proline-rich protein based on the N-terminus sequence analysis. A 65kDa inhibitor of tears with Harada's autoimmune disease was determined to be an Ig heavy chain V-III region. In addition, lactoferrin content in Harada's disease was very low. We found two cathepsin inhibitors in bovine milk using reverse zymography, namely lactoferrin and beta-casein. The L133-Q151, in the human beta-casein molecule is the active inhibitory domain. They may play an important role in antiseptic and anti-infectious functions. (Keyword) cysteine protease inhibitor / human tears / reverse zymography / Behcet's disease / lactoferrin / milk / autoimmune diseases (Tokushima University Institutional Repository) ● Metadata: 110691 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 13678384 ● Search Scopus @ Elsevier (PMID): 13678384 (Tokushima University Institutional Repository: 110691, PubMed: 13678384)
4.	Kayo Shinomiya and Hiroshi Shiota : 【ウイルス性眼疾患の診療】ウイルス感染症の治療抗ウイルス薬の種類, 眼科診療プラクティス, Vol.92, 114-117, 2003.
5.	Hiroshi Eguchi and Hiroshi Shiota : アカントアメーバ角膜炎とその治療方法について教えて下さい, Journal of the Eye, Vol.17, 181-183, 2000. (Keyword) アカントアメーバ / 角膜炎
6.	Takeshi Naito and Hiroshi Shiota : ヘルペス性角膜炎, The Japanese journal of clinical and experimental medicine, Vol.64, No.7, 27-30, Jul. 1987.
7.	Takeshi Naito and Hiroshi Shiota : 角膜ヘルペスの治療, 治療学, Vol.17, No.1, 114-117, Jul. 1986.

Proceeding of International Conference:

1.	Kayo Shinomiya, Hiroshi Shiota, Yaeko Ohgi, Nobuyuki Itsuki, Ray Tabesh, Masashi Yamada and Masanori Kubo : Analysis of the Characteristics of Electrooculogram, Intl. Conf. on Biomedical and Pharmaceutical Engineering 2006, 428-431, Singapore, Dec. 2006. (Summary) In an eyeball there is an electric potential between the cornea (positive) and retina (negative), so an eyeball can be modeled as a battery. In this report the usefulness of the model is examined by applying this battery model to the eye and estimating the weak current in the eye from the measured EOG. (Keyword) electrooculogram / Cross-talk / a battery model / electrode
2.	Hiroshi Shiota, Takeshi Naito, Hiroshi Eguchi, T Takada and S Hata : 5,3,1 METHOD FOR THE TREATMENT OF HERPETIC KERATITIS, 4th International Conference on Ocular Infections, 6, Sapporo, Oct. 2005.
3.	Hiroshi Eguchi, Kayo Shinomiya, Akiko Iwata, Shino Takano and Hiroshi Shiota : A CASE OF PANOPHTHALMITIS CAUSED BY PANTOEA AGGLOMERANS, 4th International Conference on Ocular Infections, 64, Sapporo, Oct. 2005.
4.	Hiroshi Eguchi, Kayo Shinomiya, Akiko Iwata, Shino Hayashi and Hiroshi Shiota : A Case of Panophthalmitis Caused by Pantoea Agglomerans, The 4th International eyes infectious disease society, Oct. 2005.
5.	Hiroshi Shiota : DIANOSIS AND TREATMENT OF HERPETIC KERATITIS, 4th International Conference on Ocular Infections, 4, Sapporo, Oct. 2005.
6.	Hiroshi Shiota, M Inoue, Y Kubo, Y Hayashi, Hiroshi Eguchi and Takeshi Naito : Best Classification of Acanthamoeba Keratitis, ARVO 2005 GLOBAL NETWORKING, 113, Florida, May 2005.
7.	Hiroshi Eguchi, Y Kubo, S Takano, Hiroshi Shiota and M Yano : Malignant Glaucoma After Penetrating Keratoplasty, ARVO 2004, 203, Florida, Apr. 2005.
8.	Hiroshi Shiota, M Ishimura, T Miyamoto, S Takano, E Sano, Q.T Le and N Katsunuma : Detection of disease-specific cyctein protease inhibitors in tears, ARVO 2004, 25, Florida, Apr. 2005.
9.	Hiroshi Shiota, Shino Takano, Mayumi Ishimaru, Etsuko Sano and Q.T Le : Medical significances of new disease-specific cycteine protease inhibitors in tears, 4th International Conference on Cysteine Proteinases and their Inhibitors, 6, Portoroz, Slovenia, Sep. 2004.
10.	Hiroshi Shiota : Recent diagnosis and treatment of Herpes Simplex Keratitis, FYODOROV MEMORIAL LECTURES 2004, 10, Moscow, Jun. 2004.
11.	E Sano, T. Q Le, A Ohashi, Hiroshi Shiota and N Katunuma : Disease specific cysteine protease inhibitors in tears with autoimmune-disease, International Symposium on "Medical and Biological Perspectives in Proteases and Their Inhibitors, 41, 兵庫, Nov. 2003.
12.	Hiroshi Shiota : A KERATOPROSTHESIS FOR TEMPORARY USE IN HUMANS, XXXIXth International Congress of Ophthalmology, A37, Sydney, Apr. 2002.
13.	Hiroshi Shiota, Kayo Shinomiya, T Nishiuchi, S Inoue and Takeshi Naito : ASSOCIATION OF HERPETIC KERATITIS AND KERATOMYCOSIS IN THREE PATIENTS, XXXIXth International Congress of Ophthalmology, Sydney, Apr. 2002.
14.	Hiroshi Shiota, T HIROKAWA, Takeshi Naito, F UENO, J MINAKUCHI, M KYO and K TAKAHASHI : POINTS TO DEAL WITH CYTOMEGALOVIRUS RETINITIS PATIENTS AFTER KIDNEY TRANSPLANTATION, 10th International Conference on Immunobiology and Prophylaxis of Human Herpesvirus Infections, Osaka, Nov. 2001.
15.	Hiroshi Shiota and Hiroshi Eguchi : Can we implant our keratoprosthesis for humans as a temporary use?, 4th KPro - 6th IOSS JOINT MEETING, 38, Florida, May 2001.
16.	T Hirokawa, M Hayashi, H Murata, M Takebayashi, Kyoko Fuke, T Ohki, M Kajima, Takeshi Naito, S Yamane and Hiroshi Shiota : VISUALIZATION OF PERFUSION IMAGING USING ULTRASOUND CONTRAST AGENT AND HARMONIC ULTRASOUND IN RABBIT EYES, ARVO 2001, 92, Florida, May 2001.
17.	S Hata, Takeshi Naito, M Kajima and Hiroshi Shiota : INDUCTION OF APOPTOSIS IN THE GANGLION CELLS OF TRIGEMINAL GANGLIA BY INFECTION WITH HERPES SIMPLEX VIRUS TYPE 1, ARVO 2001, 10, Florida, Apr. 2001.

Proceeding of Domestic Conference:

1.	Shoichiro Fujisawa, 三谷誠二, Osamu Sueda and Hiroshi Shiota : 徳島における視覚障害者による評価システムの構築, 第6回生活支援工学系学会連合大会講演予稿集, 187, Sep. 2008.
2.	Shoichiro Fujisawa, Osamu Sueda and Hiroshi Shiota : 徳島における視覚障害者のための社会基盤整備に関する研究, 第13回知能メカトロニクスワークショップ講演論文集, 13-18, Sep. 2008.
3.	Mitsue Takahara, Osamu Sueda, Shoichiro Fujisawa, Hiroshi Shiota, Hiroko Hata, Aya Mima, 三谷誠二 and 吉田敏昭 : Use of tactile walking surface indicators for visual impairments, 第22回リハ工学カンファレンス講演論文集, 217-218, Aug. 2007.
4.	Mitsue Takahara, Osamu Sueda, Shoichiro Fujisawa, Hiroshi Shiota, Hiroko Hata, Aya Mima, 三谷誠二 and 岩田朋巳 : Psychological Characteristics of Particpants to the Longitudinal Study, 第21回リハ工学カンファレンス講演論文集, 47-48, Aug. 2006.
5.	Shingo Matsushita, 内藤毅 and Hiroshi Shiota : Transconjunctival vitreous surgery with adjunct use of 25-guage needle, Japanese Journal of Clinical Ophthalmology, Vol.60, No.6, 1013-1015, Jun. 2006. (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-33745782922 (Elsevier: Scopus)
6.	Shoichiro Fujisawa, 三谷誠二, Aya Mima, Mitsue Takahara, 秦裕子, 湊祐史, 福永克己, Osamu Sueda and Hiroshi Shiota : Development of an evaluation system for persons with vision impairment in Tokushima, 第6回システムインテグレーション部門学術講演会, 167-168, Dec. 2005.
7.	美馬彩, Hiroko Hata, 村尾史子 and Hiroshi Shiota : 正常眼圧緑内障に対するラタノプロストの眼圧下降効果, 第59回日本臨床眼科学会, Oct. 2005.
8.	Hiroko Hata, 山根伸太 and Hiroshi Shiota : 慢性閉塞隅角緑内障に対する白内障手術とレーザー虹彩切開術の比較検討, 第59回日本臨床眼科学会, Oct. 2005.
9.	Shino Hayashi, 賀島誠, 西野真紀 and Hiroshi Shiota : 頭蓋内に進展した涙腺原発と考えられるMALTリンパ腫の1例, 第59回日本臨床眼科学会, Oct. 2005.
10.	Hiroshi Shiota : 抗ヘルペス薬眼科応用の展望, フォーサム·イン横浜, 33, Jul. 2005.
11.	Hiroshi Eguchi, 高野しの, 林勇樹, 寺田祐子, 村尾史子 and Hiroshi Shiota : 1-60 LVFX耐性Corynebacterium属が起炎菌と考えられた前眼部感染症の2例, 第42回日本眼感染症学会, 47, Jul. 2005.
12.	林勇樹, 高野しの, 賀島誠, Takeshi Naito, Hiroshi Shiota, 田近智之 and 江川麻理子 : 1-38 最近経験した内因性眼内炎の3症例, フォーサム·イン横浜, 44, Jul. 2005.
13.	西野真紀, Hiroshi Eguchi, 寺田祐子, Hiroshi Shiota, 谷英紀 and 林英憲 : 1-04 抜糸後角膜膿瘍を来した角膜移植眼の2例, フォーサム·イン横浜, 38, Jul. 2005.
14.	Hiroko Fujimoto, 山根伸太 and Hiroshi Shiota : 慢性閉塞隅角緑内障に対するPEA+IOLとLIの比較検討∼短期経過∼, 第66回徳島眼科集談会, Dec. 2004.
15.	Shino Hayashi, Takeshi Naito, 吉田慎一, 賀島誠 and Hiroshi Shiota : 徳島大学病院眼科における光線力学療法の近況, 第66回徳島眼科集談会, Dec. 2004.
16.	Shino Hayashi, 宮本龍郎, Hiroshi Shiota, 石丸真弓, 佐野悦子, Q. T. Le and 勝沼信彦 : ベーチェット病診断用の特異抗体の作成, 第108回日本眼科学会総会, Apr. 2004.
17.	Shino Hayashi, Hiroshi Eguchi, Hiroshi Shiota, R C Hicks, J G Crawford and V T Chirila : 人工角膜の歴史とAlphaCor^TM移植術の臨床成績, 第65回徳島眼科集談会, Dec. 2003.
18.	廣川富彦, 賀島誠, Takeshi Naito and Hiroshi Shiota : 334 超音波造影剤を用いた眼組織血液灌流定量化の試み, Acta Societatis Ophthalmologicae Japonicae, Vol.106, 177, May 2002.
19.	賀島誠, 廣川富彦, 三河洋一, Takeshi Naito and Hiroshi Shiota : 333 造影超音波検査のERGと蛍光眼底造影所見への影響評価, Acta Societatis Ophthalmologicae Japonicae, Vol.106, 177, May 2002.
20.	三河洋一, 廣川富彦, 賀島誠, Takeshi Naito and Hiroshi Shiota : 332 造影超音波検査の前眼部への影響評価, Acta Societatis Ophthalmologicae Japonicae, Vol.106, 177, May 2002.
21.	Kyoko Fuke, 廣川富彦, Takeshi Naito and Hiroshi Shiota : 331 カラードプラ超音波検査の角膜内皮への影響評価, Acta Societatis Ophthalmologicae Japonicae, Vol.106, 177, May 2002.
22.	Hiroshi Shiota : 角膜感染症Ⅱ 薬物の開発，その有効性, Acta Societatis Ophthalmologicae Japonicae, Vol.106, 9, May 2002.
23.	井上禎子, Kayo Shinomiya, 斬雷, Hiroshi Shiota and Eiji Kudo : 77 角膜真菌患者の角膜擦過物と涙液中からの真菌DNAの検出, Acta Societatis Ophthalmologicae Japonicae, Vol.106, 111, May 2002.
24.	村田英子, Saimoon Sharmin, Hiroshi Shiota and Hiroshi Kido : 粘液プロテアーゼインヒビターの抗アレルギー作用についての検討, Acta Societatis Ophthalmologicae Japonicae, Vol.106, 106, May 2002.
25.	坂口恭久, Shino Hayashi, 賀島誠, Takeshi Naito and Hiroshi Shiota : 徳島大学眼科における過去3年間の眼外傷の検討, 第55回日本臨床眼科学会, Oct. 2001.
26.	賀島誠, Shino Hayashi, 福家恭子, Takeshi Naito and Hiroshi Shiota : 当院における糖尿病黄斑浮腫に対する硝子体手術成績, 第55回日本臨床眼科学会, Oct. 2001.
27.	福本幸司, Shino Hayashi, 秦聡 and Hiroshi Shiota : アミロイド苔癬に合併した周辺部角膜変性症の1例, 第55回日本臨床眼科学会, Oct. 2001.
28.	Kayo Shinomiya, Shino Hayashi, 福本幸司 and Hiroshi Shiota : 両眼性網膜芽細胞腫の1例, 第67回日本中部眼科学会, Sep. 2001.
29.	林正和, Shino Hayashi, 坂口恭久 and Hiroshi Shiota : 視機能の増悪，寛解を繰り返した鼻性視神経症の1例, 第67回日本中部眼科学会, Sep. 2001.
30.	Shino Hayashi, Hiroshi Eguchi, Hiroshi Shiota, 柚木博行 and 青山茂一 : 徳島大学型人工角膜(5型)の家兎眼における成績, 第67回日本中部眼科学会, Sep. 2001.
31.	Shino Hayashi, 四宮加容, 賀島誠, Takeshi Naito and Hiroshi Shiota : 出産後改善した糖尿病網膜症の1例, 第98回中国四国眼科学会・第50回四国眼科学会合同眼科学会, Jul. 2001.
32.	林正和, Shino Hayashi, Kayo Shinomiya and Hiroshi Shiota : 白内障術後の脈絡膜剥離，眼圧上昇に対し強膜開窓術を施行した小眼球の1例, 第40回日本白内障学会・第16回日本眼内レンズ屈折手術学会, Jun. 2001.
33.	Hiroshi Eguchi, 佐藤寛之, Shino Hayashi, 竹林優 and Hiroshi Shiota : バンコマイシン眼軟膏による家兎MRSA角膜炎治療効果判断方法の病理学的検討, 第105回日本眼科学会総会, Apr. 2001.
34.	林正和, Shino Hayashi and Hiroshi Shiota : 原発閉塞隅角緑内障に対する白内障手術, 第62回徳島眼科集談会, Dec. 2000.
35.	Shino Hayashi, 福本幸司, Kayo Shinomiya, Makoto Kajima, Hiroshi Shiota, 藤江準 and Hiroko Fujimoto : 涙腺原発の腺様嚢胞癌の1例, 第62回徳島眼科集談会, Dec. 2000.
36.	林正和, 竹林優, 福家恭子, Shino Hayashi, 岩田明子, 佐藤寛之, Takashi Katome and Hiroshi Shiota : 徳島大学における最近5年間の高木式翼状片手術成績, 第54回日本臨床眼科学会, Nov. 2000.
37.	Hiroko Fujimoto, 坂口恭久, 賀島誠, Takeshi Naito and Hiroshi Shiota : ぶどう膜炎を合併した色素性傍静脈脈絡網膜萎縮症の1例, 第97回中国四国眼科学会, Aug. 2000.
38.	Hiroko Suenaga, 遠藤弘子, 竹林宏 and Hiroshi Shiota : 全層角膜移植術後の遷延性角膜上皮欠損, 第63回日本中部眼科学会, Oct. 1997.
39.	Hiroko Fujimoto, 田近智之, 賀島誠, Takeshi Naito and Hiroshi Shiota : 眼内レンズ毛様溝縫着術後に生じた網膜剥離の3例, 第58回徳島眼科集談会, Dec. 1996.

Et cetera, Workshop:

1.	西岡麻未子, 梅原麻子, Toshiyuki Obata, Hiroshi Shiota, Yousuke Ebina and Seiichi Hashida : 尿および乾燥ろ紙血による糖尿病リスク評価法の構築 1)レジスチン，2)遊離インスリン受容体α, 第49回日本生化学会中国・四国支部例会, Vol.80, No.7, 689, May 2008.
2.	Nobuyuki ITSUKI, Fumiya TAKAOKA, Yuichi MURAKAMI, Kayo Shinomiya, Masanori KUBO, Yaeko OHGI, Masashi YAMADA and Hiroshi Shiota : Consideration of Electrooculogram Characteristic Applying Eyeball Battery Model, IEICE Technical Report (MBE), Vol.107, No.154, 9-12, Jul. 2007. (Summary) We made a study of the characteristic of EOG by the battery model of eyeball. In this study we have calculated the relationship between amplitude of EOG and distance from center of a eyeball by means of battery model. And we compared this value with measured one. As a result, according to the calculation, as the eyeball approach, EOG was expected to be large sharp. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520572357248389888 (CiNii: 1520572357248389888)
3.	Kayo Shinomiya, Hiroshi Shiota, 木虎亜希子 and 岡本里江 : 3歳児健診要精査児の受診結果と問題点, 徳島県医師会報, No.412, 15-16, Sep. 2005.
4.	Hiroshi Shiota : 抗ヘルペスウイルス薬療法の先駆的研究, HERPES MANAGEMENT, Vol.8, No.1, 1-4, 2004.
5.	Hiroshi Shiota : 角膜ヘルペスの治療·いまむかし, Japanese Journal of Clinical Ophthalmology, Vol.57, No.11, 80, Oct. 2003.
6.	Hiroshi Shiota : 私の修業時代, 眼科診療プラクティス, 110-111, Feb. 2001.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

Development of a new method for the treatment of herpetic keratitis using RNA interference (Project/Area Number: 18591923 )
人工角膜・水晶体用ポリマー表面への機能性付与に関する国際共同研究の為の企画調査 (Project/Area Number: 16639017 )
Clinico-pathological elucidation of acute retinal necrosis (Project/Area Number: 15591859 )
移植デバイス用生体適合性ポリマーとその表面改質に関する国際共同研究の為の企画調査 (Project/Area Number: 14607018 )
The development of a rapid diagnostic method for keratomycosis and the establishment of a guide for its treatment (Project/Area Number: 13671841 )
Analysis of viral character in difficult herpetic keratitis (Development of quick method to detect gene mutation of DNA polymerase) (Project/Area Number: 10671646 )
ヘルペス性角膜白斑に対する人工角膜挿入術の治療方針の決定 (Project/Area Number: 07671922 )
セラミックスを用いた人口角膜の開発 (Project/Area Number: 06671766 )
角膜自己抗原の解析 (Project/Area Number: 01480417 )
クラミジアにぶどう膜炎の研究 (Project/Area Number: 63570833 )
Search by Researcher Number (20035736)

Professor Emeritus : Shiota, Hiroshi

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Et cetera, Workshop:

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)