Kazutoshi Murao, Yoshiaki Kubo, D Fukumoto, Kazuya Matsumoto and Seiji Arase : Verrucous carcinoma of the scalp associated with human papillomavirus type 33, Blackwell Publishing Ltd, Oct. 2005.
(Summary)
Verrucous carcinoma (VC) is a low-grade, well-differentiated squamous cell carcinoma of the skin or mucosae, and human papillomavirus (HPV) infection has been considered to be one of the causative factors of VC at three main sites, including the oral cavity, the genitoanal region, and the foot. However, the relationship between cutaneous VC at other sites and HPV infection remains obscure. We describe a rare case of cutaneous VC originating in a burn scar on the scalp and our attempt to find HPV infection in the lesion. We investigated the presence of HPV by polymerase chain reaction-restriction fragment length polymorphisms and immunohistochemical analysis. HPV type 33 was detected in the lesion, and positive stains for HPV were observed in several cell nuclei at the upper stratum malpighi. Since HPV type 33, as well as HPV types 16 and 18, is regarded as a high-risk, mucosal type, HPV type 33 infection likely contributed to the development of the lesion. We suggest that HPV infection should be relevant to a subset of cutaneous VC.
Kazutoshi Murao, Yoshiaki Kubo, Kosaku Fukuhara, Kazuya Matsumoto and Seiji Arase : Three cases of Bowen's disease on the lower abdomen associated with high-risk types 16, 33, and 59 of human papillomavirus, Elsevier Science, Apr. 2005.
Kazutoshi Murao, Yoshiaki Kubo, Hirotsugu Takiwaki, Seiji Arase and Kazuya Matsumoto : Bowen's disease on the sole: p16INK4a overexpression associated with human papillomavirus type 16, Blackwell Publishing Ltd, Jan. 2005.
(Summary)
We report a case of Bowen's disease on the sole presenting clinically as an exophytic, blackish-grey, verrucous tumour, and showing human papillomavirus (HPV) type 16 on analysis with polymerase chain reaction. Positive stains for HPV particles by immunohistochemical analysis were limited to several cell nuclei at the upper stratum Malpighii. However, all the tumour cells in the epidermis exhibited strong and diffuse nuclear and cytoplasmic stains for the tumour suppressor protein p16INK4a. We speculate that dysregulation of the retinoblastoma/p16INK4a pathway may be involved in the pathogenesis of the lesion, and p16INK4a overexpression might serve as a useful surrogate marker for identifying Bowen's disease harbouring high-risk types of HPV infection.
Kazutoshi Murao, Mayu Yuasa, Takeshi Ishigami, Yoshiaki Kubo and Seiji Arase : Bowen disease of the palm associated with human papillomavirus 52., Clinical and Experimental Dermatology, Vol.38, No.5, 489-491, 2013.
(Summary)
Human papillomavirus (HPV) is a well-known risk factor for many human cancers, especially cervical cancers. Among the nonmelanoma skin cancers, Bowen disease (BD) of the genitalia and fingers has also been shown to be closely associated with the high-risk types of HPV, especially HPV16. We report a case of BD of the palm, which is a very rare location for BD. In addition to its rare location, HPV52, which is classified as a mucous high-risk HPV type, was detected in the lesion by PCR restriction fragment length polymorphism analysis. To our knowledge, this is the first reported case of BD associated with HPV52.
Mika Oshima, Kazutoshi Murao and Seiji Arase : Drug-induced skin damage by intravenous administration of Nifekalant hydrochloride, Hifuka No Rinsho, Vol.54, No.1, 55-57, 2012.
Daisuke Fukumoto, Shin-ichi Ansai, 福本 実扶子, Yoshiaki Kubo, Seiji Arase and Hideki Nakanishi : A clinicopathological study of primary cutaneous invasive squamous cell carcinoma: relationship between a new clinicopathological classification and program, The Japanese Journal of Dermatology, Vol.121, No.11, 2247-2255, 2011.
(Summary)
We performed a clinicopathological analysis of 305 cases of primary cutaneous invasive squamous cell carcinoma (SCC) diagnosed in Tokushima University hospital in the past 25 years. We proposed a new clinicopathological classification of SCC based on epidemiological factors and divided it into nine categories; actinic keratosis type, bowen type, keratoacanthomatous type, cystic type, genital type, cicatricial type, radiodermatitis type, xeroderma pigmentosa type, and miscellaneous type. We examined the clinicopathological characteristics in each category such as favorite locations and prognosis. Among the clinical characteristics of those cases, such as sex ratio and age at the time of the excision, the favorite sites were similar to those in former reports in Japan. The actinic keratosis type is a significant type with little metastasis, but the radiodermatitis type and the genital type showed high metastatic rates. The case that occurred on the face showed little metastasis, but the cases on the lower limbs and genitalia had high metastatic rates. Cases with a tumor thickness of less than 4 mm showed lower metastatic rates than those with a tumor thickness of more than 4 mm. We divided those 25 years into five year segments as investigation periods and examined the numbers of cases, sex ratios, and the frequencies of the actinic keratosis type, the Bowen disease type, and the other types and found that these factors were essentially equal.
(Keyword)
Squamous cell carcinoma / SCC / Clinicopathological study
Yoshiaki Kubo, Yoshio Urano, Rie Matsuda, Takeshi Ishigami, Kazutoshi Murao, Seiji Arase and Akemi Ishida-Yamamoto : Ichthyosis hystrix, Curth-Macklin type: a new sporadic case with a novel mutation of keratin 1., Archives of Dermatology, Vol.147, No.8, 999-1001, 2011.
Mio Machida, Yasutoshi Hida, Yoshiaki Kubo, Seiji Arase and 安齋 眞一 : Clinical case reports: Coexistence of low-grade sebaceous carcinoma and basal cell carcinoma in a nevus sebaceous, The Nishinihon Journal of Dermatology, Vol.73, No.2, 152-156, 2011.
(Summary)
A 60-year-old Japanese man visited our outpatient clinic complaining of a black protruding tumor on his left cheek with a 3-year history. The tumor arose in a pre-existing skin-colored plaque that had been noticed since his childhood. We suspected a basal cell carcinoma (BCC) arising in a nevus sebaceus (NS), and excised the lesion including the NS. A histopathological examination of the whole specimen demonstrated the coexistence of a low-grade sebaceous carcinoma (low-grade SC) and BCC in an NS. An NS is classified into three progression stages, and various types of epithelial tumors occur secondarily in the lesion at the third stage, especially when patients are more than 50 years old. Among those secondary malignant tumors, BCC is the most common, and SC is rather rare. We reviewed the literature and summarized 31 cases of malignant sebaceous tumors arising in an NS, focusing on clinical characteristics. Surprisingly, seven cases had other malignant tumors, most of which were BCC as in our case. Only one patient with SC in an NS died from metastasis, and another 30 cases were cured after appropriate treatments. Therefore, we suggest that SC in an NS is curable by careful extirpation, although the prognosis of common SC is rather poor. But seen on the background of this case the concept of `low-grade SC' should be kept in mind.
Sachiko Takeichi, Yoshiaki Kubo, Kazutoshi Murao, Natsuko Inoue, Shin-ichi Ansai and Seiji Arase : Coexistence of giant blue nevus of the scalp with hair loss and alopecia areata., The Journal of Dermatology, Vol.38, No.4, 377-381, 2010.
(Summary)
A 43-year-old Japanese man presented with a 13-year history of a grayish macule measuring 7 cm in diameter with sparse hairs on the vertex. Histopathological examination demonstrated two types of melanocytes, spindle-shaped and ovoid cells, with abundant melanin aggregated around the upper part of the pilosebaceous apparatus. Fibrous, thick collagen bundles were also seen surrounding the upper part of the small hair follicles. There was no infiltration of melanocytes or lymphocytes in the lower dermis or adipose tissue. Based on these findings, a diagnosis of blue nevus, cellular type, was made. Giant cellular blue nevi on the scalp are rare, and 11 cases reported in the published work have shown characteristic features such as hair loss and cranial invasion of nevus cells. It should be noted that melanocytes of giant blue nevi have a high potential to damage other organs such as underlying bone and hair follicles. The patient also showed a typical lesion of alopecia areata on the left temporal which was successfully treated with topical corticosteroid.
(Keyword)
Adult / Alopecia Areata / Humans / Male / Nevus, Blue / Scalp / Skin Neoplasms / Tomography, X-Ray Computed
Mio Machida, Shin-ichi Ansai, Yasutoshi Hida, Yoshiaki Kubo, Seiji Arase and Ken Kuramoto : Basal cell carcinoma arising on the palm., The Journal of Dermatology, Vol.38, No.1, 94-96, 2010.
Yoshiaki Kubo, Daisuke Fukumoto, Takeshi Ishigami, Yasutoshi Hida and Seiji Arase : Diltiazem-associated photodistributed hyperpigmentation: report of two Japanese cases and published work review., The Journal of Dermatology, Vol.37, No.9, 807-811, 2010.
(Summary)
Scherschum et al. proposed diltiazem-associated photodistributed hyperpigmentation as a novel type of drug-induced photosensitive lichenoid eruption. The characteristic clinical features were slate-gray reticulated hyperpigmentation on sun-exposed areas, while lichenoid dermatitis with prominent pigmentary incontinence was noted histologically. Although the clinical and histological features were similar to those of lichen planus pigmentosus, the histological features did not show either compact hyperkeratosis or wedge-shaped hypergranulosis, which are typical histological features of lichen planus. We describe two Japanese cases of diltiazem-associated photodistributed hyperpigmentation, who were successfully treated with topical tacrolimus, and review the published work.
(Keyword)
Aged, 80 and over / Asian Continental Ancestry Group / Calcium Channel Blockers / Diltiazem / Drug Eruptions / Female / Humans / Hyperpigmentation / Japan / Photosensitivity Disorders / Tacrolimus / Treatment Outcome
Kazuya Matsumoto, Ichiro Hashimoto, Hideki Nakanishi, Yoshiaki Kubo, Kazutoshi Murao and Seiji Arase : Resin splint as a new conservative treatment for ingrown toenails., The Journal of Medical Investigation : JMI, Vol.57, No.3-4, 321-325, 2010.
(Summary)
Several conservative as well as surgical methods are used for the treatment of ingrown toenails until date. The conservative methods are either based on nail splinting or on orthonyxia, but no methods employing both principles have been reported thus far. Moreover, surgical methods usually involve postoperative pain, prolonged wound healing and restricted activities of daily living. Therefore, considering the need of a simplified, non-invasive method, in this study, we applied a novel splint to treat patients with ingrown toenails and estimated the clinical efficacy as well as rate of recurrence following treatment. The splint is a plate made of resin that is attached to the lateral edge of the nail using a bandage. We studied 61 patients (19 men and 42 women; mean age 36 years), with an average application duration of 9.3 months and an average follow-up period of 10 months in all patients. All patients experienced pain relief within a week of splint application and a decrease in the degree of nail deformity. Moreover, follow-up revealed a recurrence rate of 8.2%. Therefore, we believe that this new device is an excellent conservative treatment method for patients with ingrown toenails.
Kazuya Matsumoto, Ichiro Hashimoto, Shinji Matsuo, Hideki Nakanishi, Yoshiaki Kubo, Kazutoshi Murao and Seiji Arase : Prevention of pressure ulcers in home care patients, Shikoku Acta Medica, Vol.65, No.5.6, 137-140, 2009.
(Summary)
A large proportion of home care patients are at risk of pressure ulcer. The occurrence rate of pressure ulcer is higher in home care patients than that in the patients of hospitals and welfare facilities. The home care patients need long-term conservative treatment because of the depth of the pressure ulcers.Treatment of the pressure ulcers at home results in increase of caregivers burden. Therefore,prevention and early detection of pressure ulcers are important in home nursing. In early stage of pressure ulcers，``deep tissue injury''should be taken into consideration because of its progressive nature. It is a new concept which presents deep tissue damage remaining superficial skin intact.One of clinical features in this condition is induration in a deep layer.The past decade has seen a huge growth in the application of pressure relieving mattresses,absorbent products for urinary and faecal incontinence, and skin barrier products to prevention of the pressure ulcers. Economical, physical, and psychological burden of nursing at home should be decreased with an accumulation of these information and utilization of the products. Therefore, it is important for caregivers to consult with their care managers and acquire new knowledge and information.
(Keyword)
pressure sore / skin care / deep tissue injury / caregivers
Daisuke Fukumoto, Yoshiaki Kubo, Minako Saito and Seiji Arase : Centrifugal lipodystrophy of the scalp presenting with an arch-form alopecia: a 10-year follow-up observation., The Journal of Dermatology, Vol.36, No.9, 499-503, 2009.
(Summary)
We describe a 10-year follow-up observation of progressive arch-form alopecia caused by centrifugal lipodystrophy (CLD) in a Japanese boy. A 2.5-year-old boy developed a slightly depressed lesion demarcated by a horseshoe-shaped erythematous border on his right neck, which then extended to the scalp. Four years later, arch-form alopecia became apparent in the right temporal region along with an erythematous border. The arch-form alopecia gradually expanded centrifugally, leaving a slight residual depression, but hair regrowth was seen within the area of alopecia. Histological examination of the erythematous border revealed non-specific inflammatory changes in the subcutaneous fat. Magnetic resonance imaging findings revealed a loss of subcutaneous fat inside the lesion. The alopecia continuously extended until he was 12 years old, but, thereafter, expansion ceased and hair regrowth gradually occurred in the arch-form alopecia. A linear non-hairy lesion 5 cm in length still remained when he was 13 years old. CLD might involve the scalp and cause linear, arch-form alopecia.
(Keyword)
Adolescent / Age Factors / Alopecia / Histiocytes / Humans / Lipodystrophy / Lymphocytes / Magnetic Resonance Imaging / Male / Panniculitis / Scalp Dermatoses
Kimi Yamakoshi, Akiko Takahashi, Fumiko Hirota, Rika Nakayama, Naozumi Ishimaru, Yoshiaki Kubo, J. David Mann, Masako Ohmura, Atsushi Hirao, Hideyuki Saya, Seiji Arase, Yoshio Hayashi, Kazuki Nakao, Mitsuru Matsumoto, Naoko Ohtani and Eiji Hara : Real-time in vivo imaging of p16Ink4a reveals cross-talk with p53, The Journal of Cell Biology, Vol.186, No.3, 393-407, 2009.
(Summary)
Expression of the p16(Ink4a) tumor suppressor gene, a sensor of oncogenic stress, is up-regulated by a variety of potentially oncogenic stimuli in cultured primary cells. However, because p16(Ink4a) expression is also induced by tissue culture stress, physiological mechanisms regulating p16(Ink4a) expression remain unclear. To eliminate any potential problems arising from tissue culture-imposed stress, we used bioluminescence imaging for noninvasive and real-time analysis of p16(Ink4a) expression under various physiological conditions in living mice. In this study, we show that oncogenic insults such as ras activation provoke epigenetic derepression of p16(Ink4a) expression through reduction of DNMT1 (DNA methyl transferase 1) levels as a DNA damage response in vivo. This pathway is accelerated in the absence of p53, indicating that p53 normally holds the p16(Ink4a) response in check. These results unveil a backup tumor suppressor role for p16(Ink4a) in the event of p53 inactivation, expanding our understanding of how p16(Ink4a) expression is regulated in vivo.
Sachiko Takeichi, Yoshiaki Kubo, Seiji Arase, Takashi Hashimoto and Shin-ichi Ansai : Brunsting-Perry type localized bullous pemphigoid, possibly induced by furosemide administration and sun exposure., European Journal of Dermatology : EJD, Vol.19, No.5, 500-503, 2009.
(Summary)
A 56-year-old male visited our hospital complaining of irregular-shaped atrophic erythematous plaques with blood crust, erosion, pigmentation, and depigmentation, localized on his forehead, bilateral cheeks, and ear lobes, for over one year. He has been receiving peritoneal dialysis and furosemide administration due to chronic renal failure for four years. Direct immunofluorescence examination exhibited linear depositions of IgG and C3 in the basement membrane zone. Antibodies against the recombinant NC16a-domain of BP180 were negative by enzyme-linked immunosorbent assays (ELISA), though BP230 ELISA was positive. Immunoblotting using extracts of normal human epidermis demonstrated that the patient's serum reacted with BP180 and BP230. IgG class autoantibodies to recombinant proteins of the C-terminal domain of BP180 were also detected by immunoblotting. This case was diagnosed as localized bullous pemphigoid (LBP). Oral administration of prednisolone 10 mg daily was started and furosemide administration was ceased. The eruptions disappeared thereafter with superficial scars. This is the second reported case of Brunsting-Perry type LBP associated with IgG class autoantibodies to the C-terminal domain of BP180. Furthermore, photosensitivity caused by furosemide administration may contribute to the induction and exacerbation of the lesions.
Yasutoshi Hida, Yoshiaki Kubo, Osamu Miyajima and Seiji Arase : Primary dermal melanoma: A case report and molecular characterization., The Journal of Dermatology, Vol.36, No.6, 346-352, 2009.
(Summary)
Swetter et al. proposed primary dermal melanoma (PDM) as a distinct entity based on an excellent prognosis. The histopathological features of PDM are extremely similar to those of metastatic melanoma or clear cell sarcoma (CCS). We describe a 38-year-old woman with a subcutaneous tumor in her left thigh. Physical and imaging examinations showed no evidence of metastatic melanoma. The lesion showed obvious strong expression of KIT by immunohistochemistry, but no EWS-ATF1 fusion transcript specific for CCS was detected by reverse transcription polymerase chain reaction. In further analyses of KIT expression in other tumors, three of four primary melanomas (75%) and six of 12 metastatic melanomas (50%) were moderately or strongly positive, however, both the primary and metastatic lesions of CCS tested negative. We believe this to be a case of PDM, and emphasize the distinctiveness of PDM.
Eun Ju Jung, Tomoko Kawai, Hwan Ki Park, Yoshiaki Kubo, Kazuhito Rokutan and Seiji Arase : Identification of ultraviolet B-sensitive genes in human peripheral blood cells., The Journal of Medical Investigation : JMI, Vol.55, No.3-4, 204-210, 2008.
(Summary)
Ultraviolet B (UVB) is a serious irritant for the skin and increases a risk for skin cancer. To identify UVB-sensitive genes in peripheral blood, 11 healthy male volunteers were exposed to 0.3 J/cm(2) of narrow-band (NB)-UVB, about half of minimal erythema dose (MED) in Japanese, and gene expression in blood was analyzed at 4 h, 24 h, 4 d and 7 d after the irradiation using microarray carrying oligonucleotide probes for 2,000 stress-responsive genes. RNA prepared before the irradiation was used as a reference control. Microarray analysis identified 21 genes as UVB-responsive genes with a peak at 24 h in 6 subjects, and real-time PCR validated the significant down-regulation of 9 (ABCB10, ATF1, ABCD3, TANK, FAS, SLC30A9, CHUK, CASP1, and ABCE1) out of the 21 genes in 11 subjects. Considering sensitive and characteristic features of 9 marker genes, they may be useful indicators for monitoring systemic response to UVB irradiation.
Mitsuru Matsumoto, Yiqing Zhou, Shinji Matsuo, Hideki Nakanishi, Kenji Hirose, Hajimu Oura, Seiji Arase, Akemi Ishida-Yamamoto, Yoshimi Bando, Keisuke Izumi, Hiroshi Kiyonari, Naoko Oshima, Rika Nakayama, Akemi Matsushima, Fumiko Hirota, Yasuhiro Mouri, Noriyuki Kuroda, Shigetoshi Sano and David D. Chaplin : Targeted deletion of the murine corneodesmosin gene delineates its essential role in skin and hair physiology, Proceedings of the National Academy of Sciences of the United States of America, Vol.105, No.18, 6720-6724, 2008.
(Summary)
Controlled proteolytic degradation of specialized junctional structures, corneodesmosomes, by epidermal proteases is an essential process for physiological desquamation of the skin. Corneodesmosin (CDSN) is an extracellular component of corneodesmosomes and, although considerable debate still exists, genetic studies have suggested that the CDSN gene in the major psoriasis-susceptibility locus (PSORS1) may be responsible for susceptibility to psoriasis, a human skin disorder characterized by excessive growth and aberrant differentiation of keratinocytes. CDSN is also expressed in the inner root sheath of hair follicles, and a heterozygous nonsense mutation of the CDSN gene in humans is associated with scalp-specific hair loss of poorly defined etiology. Here, we have investigated the pathogenetic roles of CDSN loss of function in the development of skin diseases by generating a mouse strain with targeted deletion of the Cdsn gene. Cdsn-deficient mouse skin showed detachment of the stratum corneum from the underlying granular layer and/or detachment within the upper granular layers due to the disrupted integrity of the corneodesmosomes. When grafted onto immunodeficient mice, Cdsn-deficient skin showed rapid hair loss together with epidermal abnormalities resembling psoriasis. These results underscore the essential roles of CDSN in hair physiology and suggest functional relevance of CDSN gene polymorphisms to psoriasis susceptibility.
Rie Yamamura, Noriyuki Nishimura, Hiroyoshi Nakatsuji, Seiji Arase and Takuya Sasaki : The Interaction of JRAB/MICAL-L2 with Rab8 and Rab13 Coordinates the Assembly of Tight Junctions and Adherens Junctions., Molecular Biology of the Cell, Vol.19, No.3, 971-983, 2008.
(Summary)
The assembly of tight junctions (TJs) and adherens junctions (AJs) is regulated by the transport of integral TJ and AJ proteins to and/or from the plasma membrane (PM) and it is tightly coordinated in epithelial cells. We previously reported that Rab13 and a junctional Rab13-binding protein (JRAB)/molecule interacting with CasL-like 2 (MICAL-L2) mediated the endocytic recycling of an integral TJ protein occludin and the formation of functional TJs. Here, we investigated the role of Rab13 and JRAB/MICAL-L2 in the transport of other integral TJ and AJ proteins claudin-1 and E-cadherin to the PM by using a Ca(2+)-switch model. Although knockdown of Rab13 specifically suppressed claudin-1 and occludin but not E-cadherin transport, knockdown of JRAB/MICAL-L2 and expression of its Rab13-binding domain (JRAB/MICAL-L2-C) inhibited claudin-1, occludin, and E-cadherin transport. We then identified Rab8 as another JRAB/MICAL-L2-C-binding protein. Knockdown of Rab8 inhibited the Rab13-independent transport of E-cadherin to the PM. Rab8 and Rab13 competed with each other for the binding to JRAB/MICAL-L2 and functionally associated with JRAB/MICAL-L2 at the perinuclear recycling/storage compartments and PM, respectively. These results suggest that the interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of AJs and TJs.
(Keyword)
Adherens Junctions / Animals / Binding, Competitive / Cadherins / Cell Line / Cytoskeletal Proteins / Endocytosis / Humans / Intracellular Space / Membrane Proteins / Models, Biological / Protein Binding / Protein Structure, Tertiary / Protein Transport / Tight Junctions / rab GTP-Binding Proteins
Ichiro Hashimoto, Hiromichi Sedo, Kei Inatsugi, Hideki Nakanishi and Seiji Arase : Severe radiation-induced injury after cardiac catheter ablation:a case requiring free anterolateral thigh flap and vastus lateralis muscle flap reconstruction on the upper arm, Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS, Vol.61, No.6, 704-708, 2008.
(Summary)
Although cases of radiation-induced skin injury after fluoroscopically-guided procedures have been reported since 1996, diagnosis and treatment of such injury remain difficult. We present the case of a patient who complained of two ulcers, one on his right arm and one on his back, and limited motion of the right elbow joint after repeated cardiac radiofrequency catheter ablations. After resection of the skin ulcer on the patient's arm and of degenerated tissue in the distal part of the triceps brachii muscle, a combined free flap incorporating an anterolateral thigh flap and vastus lateralis muscle flap was transplanted. In the present case, this combined flap was very useful for simultaneous reconstruction of the muscle and skin defects under and over the tendon because the muscle flap and skin flap could be arranged separately.
Naoko Ohtani, Yuko Imamura, Kimi Yamakoshi, Fumiko Hirota, Rika Nakayama, Yoshiaki Kubo, Naozumi Ishimaru, Akiko Takahashi, Atsushi Hirao, Takatsune Shimizu, David J. Mann, Hideyuki Saya, Yoshio Hayashi, Seiji Arase, Mitsuru Matsumoto, Nakao Kazuki and Eiji Hara : Visualizing the dynamics of p21 (Wafl/Cip1)cyclin-dependent kinase inhibitor expression in living animals., Proceedings of the National Academy of Sciences of the United States of America, Vol.104, No.38, 15034-15039, 2007.
(Summary)
Although the role of p21(Waf1/Cip1) gene expression is well documented in various cell culture studies, its in vivo roles are poorly understood. To gain further insight into the role of p21(Waf1/Cip1) gene expression in vivo, we attempted to visualize the dynamics of p21(Waf1/Cip1) gene expression in living animals. In this study, we established a transgenic mice line (p21-p-luc) expressing the firefly luciferase under the control of the p21(Waf1/Cip1) gene promoter. In conjunction with a noninvasive bioluminescent imaging technique, p21-p-luc mice enabled us to monitor the endogenous p21(Waf1/Cip1) gene expression in vivo. By monitoring and quantifying the p21(Waf1/Cip1) gene expression repeatedly in the same mouse throughout its entire lifespan, we were able to unveil the dynamics of p21(Waf1/Cip1) gene expression in the aging process. We also applied this system to chemically induced skin carcinogenesis and found that the levels of p21(Waf1/Cip1) gene expression rise dramatically in benign skin papillomas, suggesting that p21(Waf1/Cip1) plays a preventative role(s) in skin tumor formation. Surprisingly, moreover, we found that the level of p21(Waf1/Cip1) expression strikingly increased in the hair bulb and oscillated with a 3-week period correlating with hair follicle cycle progression. Notably, this was accompanied by the expression of p63 but not p53. This approach, together with the analysis of p21(Waf1/Cip1) knockout mice, has uncovered a novel role for the p21(Waf1/Cip1) gene in hair development. These data illustrate the unique utility of bioluminescence imaging in advancing our understanding of the timing and, hence, likely roles of specific gene expression in higher eukaryotes.
Hirotsugu Takiwaki, Yuki Miyaoka and Seiji Arase : Analysis of the absorbance spectra of skin lesions as a helpful tool for detection of major pathophysiological changes, Skin Research and Technology, Vol.10, No.2, 130-135, 2004.
(Summary)
Although reflectance spectrophotometry is often applied to measurement of skin color, raw data of reflectance spectra of normal and lesional skin are difficult to analyze. The purpose of this pilot study was to determine whether measurement of spectral difference in absorbance (SDA) between various skin lesions and normal skin adjacent to them could yield useful information for clinics in dermatology. We studied spectral reflectance of a total of 173 various skin lesions. After converting obtained reflectance into apparent absorbance A (=log(10)(1/reflectance)), we examined the profile of SDA, that is, A(lesion)-A(normal skin) in the range of 400-700 nm, and compared them with the absorbance spectra of melanin and hemoglobin. SDA of epidermal pigmentary disorders was similar to the absorption spectrum of melanin in vitro, but the cases with intradermal melanin deposition showed a different pattern, reflecting the scattering effect of the dermis. SDA of erythematous lesions was similar to the spectra of either oxygenated or reduced hemoglobin, and varied according to the oxygenated level of cutaneous blood. SDA of lesions with a combination of factors appeared as a simple summation of spectra corresponding to each of the factors. Our method may offer easy and quick detection of major pathophysiological changes in skin lesions.
Hirotsugu Takiwaki, H Tsuda, Seiji Arase and H Takeichi : Difference between intrafollicular microorganism profiles in perioral and seborrhoeic dermatitis, Clinical and Experimental Dermatology, Vol.28, No.5, 531-534, 2003.
(Summary)
Although often associated with overuse of topical corticosteroids, perioral dermatitis seems to develop seldom in patients with typical seborrhoeic dermatitis in spite of long-term application of corticosteroids. In order to compare the profiles of intrafollicular microorganisms in the lesions of perioral and seborrhoeic dermatitis, tape-stripped samples were obtained from eight lesions of perioral dermatitis, 10 lesions of seborrhoeic dermatitis, and the perioral skin of 31 normal subjects. After staining with Toluidine blue, resident microorganisms on plucked hair roots were evaluated microscopically. In all patients with perioral dermatitis and two normal subjects, 20-70% of sample hairs were positive for fusiform bacteria regarded as fusobacteria. Malassezia-positive hairs were rarely seen in these cases. Seborrhoeic dermatitis showed the opposite results. Perioral dermatitis may tend to develop under fusiform-bacteria-rich conditions, rather than Malassezia-rich conditions as in the case of seborrhoeic dermatitis.
Takuya Seike, Kazuya Matsumoto, Hideki Nakanishi, Ichiro Hashimoto, Yoshiaki Kubo and Seiji Arase : Malignant Melanoma of Soft Parts (Clear Cell Sarcoma), --- A Case Report ---, The Journal of Dermatology, Vol.30, No.7, 550-555, 2003.
(Summary)
A subcutaneous tumor localized on the sole of the foot of a 25-year-old Japanese man was diagnosed as a malignant melanoma of the soft parts. We performed a wide local excision, elective lymph node dissection, and chemotherapy. Eight years after treatment, the patient is alive without local recurrence or metastasis.
Yoshiaki Kubo, Kazutoshi Murao, Kazuya Matsumoto and Seiji Arase : Molecular carcinogenesis of squamous cell carcinomas of the skin., The Journal of Medical Investigation : JMI, Vol.49, No.3-4, 111-117, 2002.
(Summary)
Squamous cell carcinomas (SCCs) of the skin were suggested to develop through a multistep process that involves activation of proto-oncogenes and/or inactivation of tumor suppressor genes in the human skin keratinocytes. Exposure to ultra-violet (UV), especially UV-B, radiation is the most common cause for these genetic abnormalities in cells. We review causation of SCCs and genetic abnormalities in human SCCs with the current work. To elucidate the multistep process, we developed a method for examining the combinatorial function in vivo of plural genes in human keratinocytes. Using high efficiency retroviral transductions, we could express plural genes serially in normal human primary keratinocytes and use these cells to regenerate human skin on SCID mice. A combinatorial transduction of H-RasV12 and cyclin dependent kinase 4 (CDK4) produced human epidermal neoplasia resembling SCC. These findings were consistent with our previous results of mutation analysis in SCCs, one of which had both mutations of H-Ras gene and the INK4a locus. Therefore, it is suggested that a combination of these genetic abnormalities might be crucial to the carcinogenesis at least in a subset of SCCs.
Hirotsugu Takiwaki, Y Miyaoka, N Skrebova, H Kohno and Seiji Arase : Skin reflectance-spectra and colour-value dependency on measuring-head aperture area in ordinary reflectance spectrophotometry and tristimulus colourimetry, Skin Research and Technology, Vol.8, No.2, 94-97, 2002.
(Summary)
Measurement of skin colour has become increasingly popular in the study of dermatology with the increased availability of portable instruments. However, different instruments have been reported as giving different results from measurement of the same skin region. The aim of this study was to examine the effects of differences in measuring-head aperture area on skin reflectance spectra and colour values. We measured both reflectance spectra and CIE-L*a*b* values of the skin in five different anatomical regions on 10 subjects using two MINOLTA reflectance spectrometers that were identical apart from the aperture area of the measuring heads (diameters: 5 and 11 mm). For comparison, data were also obtained from a skin-coloured tile. Skin reflectance values measured with the wider-aperture instrument were higher than those measured with the other, irrespective of anatomical location. The differences between the two were near zero at an incident light wavelength of 400 nm, but increased to around 10% of the reflectance value at 700 nm, increasing exponentially with incident light of increasing wavelength. Skin colour was observed to be brighter, redder and yellower, in CIE-L*a*b* expression, when measured with the wider-aperture instrument. The differences between measurements obtained from the skin-coloured tile were much smaller. Skin reflectance and colour values measured with reflectance instruments are not absolute data but depend on the aperture area of the measuring head. This is probably due to variations in the proportion of longer-wavelength light reflected from the skin and collected by the instrument.
Ming Li, Azusa Marubayashi, Yutaka Nakaya, Kiyoshi Fukui and Seiji Arase : Minoxidil-Induced hair growth is mediated by adenosine in cultured dermal papilla cells: possible involvement of sulfonylurea receptor 2B as a target of minoxidil, The Journal of Investigative Dermatology, Vol.117, No.6, 1594-1600, 2001.
(Summary)
The mechanism by which minoxidil, an adenosine-triphosphate-sensitive potassium channel opener, induces hypertrichosis remains to be elucidated. Minoxidil has been reported to stimulate the production of vascular endothelial growth factor, a possible promoter of hair growth, in cultured dermal papilla cells. The mechanism of production of vascular endothelial growth factor remains unclear, however. We hypothesize that adenosine serves as a mediator of vascular endothelial growth factor production. Minoxidil-induced increases in levels of intracellular Ca(2+) and vascular endothelial growth factor production in cultured dermal papilla cells were found to be inhibited by 8-sulfophenyl theophylline, a specific antagonist for adenosine receptors, suggesting that dermal papilla cells possess adenosine receptors and sulfonylurea receptors, the latter of which is a well-known target receptor for adenosine-triphosphate-sensitive potassium channel openers. The expression of sulfonylurea receptor 2B and of the adenosine A1, A2A, and A2B receptors was detected in dermal papilla cells by means of reverse transcription polymerase chain reaction analysis. In order to determine which of the adenosine receptor subtypes contribute to minoxidil-induced hair growth, the effects of subtype-specific antagonists for adenosine receptors were investigated. Significant inhibition in increase in intracellular calcium level by minoxidil or adenosine was observed as the result of pretreatment with 8-cyclopentyl-1,3-dipropylxanthine, an antagonist for adenosine A1 receptor, but not by 3,7-dimethyl-1-propargyl-xanthine, an antagonist for adenosine A2 receptor, whereas vascular endothelial growth factor production was blocked by both adenosine A1 and A2 receptor antagonists. These results indicate that the effect of minoxidil is mediated by adenosine, which triggers intracellular signal transduction via both adenosine A1 and A2 receptors, and that the expression of sulfonylurea receptor 2B in dermal papilla cells might play a role in the production of adenosine.
(Tokushima University Institutional Repository: 110488)
45.
Yoshiaki Kubo, Yoshio Urano, Yasutoshi Hida, Tsuneo Ikeuchi, Masashi Nomoto, Kazufumi Kunitomo and Seiji Arase : A novel PTEN mutation in a Japanese patient with Cowden disease., The British Journal of Dermatology, Vol.142, No.6, 1100-1105, 2000.
(Summary)
Cowden disease (CD) is an autosomal dominant syndrome characterized by multiple hamartomatous lesions and an increased risk for malignancies. Recent evidence has indicated that the PTEN gene, encoding a protein tyrosine phosphatase, is the CD susceptibility gene. However, another line of evidence has suggested that CD might be genetically heterogeneous. Clinical features of CD are variable, and there are interfamilial differences in the expression of skin lesions. Therefore, information on PTEN mutations in CD patients should be accumulated to clarify the genotype-phenotype correlation. In the present study, we found heterozygous germline mutations of PTEN in all of three Japanese patients with CD examined, indicating no genetic heterogeneity among our patients. The mutations included two non-sense mutations of R335X and R130X, and a mis-sense mutation of C136R. To the best of our knowledge, the C136R mutation has not previously been reported in CD patients. This novel mutation was located outside the core motif of the phosphatase domain of PTEN protein, where most of the missense mutations previously reported in CD patients were clustered. Mucocutaneous manifestations were far fewer in the patient with this mutation than in the patients with nonsense mutations. Whether the phenotypic difference in mucocutaneous features was due to the different mutations remains unclear.
Yoshiaki Kubo, Yoshio Urano, Yasutoshi Hida and Seiji Arase : Lack of somatic mutation in the PTEN gene in squamous cell carcinomas of human skin., Journal of Dermatological Science, Vol.19, No.3, 199-201, 1999.
(Summary)
The tumor suppressor gene PTEN is deleted and/or mutated in a variety of tumors and the susceptibility gene for Cowden disease. Loss of heterozygosity of chromosome 10q23, where PTEN resides, in squamous cell carcinomas (SCCs) of human skin and the association of SCC with Cowden disease were reported previously. In the present study, we screened for mutations of PTEN in SCCs by polymerase chain reaction single strand conformation polymorphism analysis to examine whether PTEN is involved in the carcinogenesis of SCC. None of 21 SCCs showed somatic mutations in the coding regions of PTEM. Instead the same allelic variation was detected in two cases without any clinical features of Cowden disease. Our results indicate that inactivation of PTEN does not play an important role in the carcinogenesis of SCC.
Ichiro Hashimoto, Y. Urano, Hideki Nakanishi, Hajimu Oura, S. Kato and Seiji Arase : Cerebriform intradermal nevus:A case of scalp expansion on the galea., The Journal of Dermatology, Vol.26, No.4, 258-263, 1999.
48.
Ichiro Hashimoto, Urano Yoshio, Hideki Nakanishi, Oura Hajime, Kato Shouji and Seiji Arase : Cerebriform Intradermal Nevus, --- A Case of Scalp Expansion on the Gales ---, The Journal of Dermatology, Vol.26, No.4, 258-263, 1999.
49.
Yoshiaki Kubo, Yoshio Urano, Kosaku Fukuhara, Kazuya Matsumoto and Seiji Arase : Lack of mutation in the INK4a locus in basal cell carcinomas., The British Journal of Dermatology, Vol.139, No.2, 340-341, 1998.
Kazuya Matsumoto, Yoshio Urano, Yoshiaki Kubo, Hideki Nakanishi and Seiji Arase : Mutation of the fibrobrast growth factor receptor2 gene in Japanese patients with Apert syndrome, Plastic and Reconstructive Surgery, Vol.101, No.2, 307-311, 1998.
52.
Yoshiaki Kubo, Yoshio Urano, Kazuya Matsumoto, Kamrul Ahsan and Seiji Arase : Mutations of the INK4a locus in squamous cell carcinomas of human skin., Biochemical and Biophysical Research Communications, Vol.232, 38-42, 1997.
53.
Yoshihiro Ninomiya, Yoshio Urano, Katsuhiko Yoshimoto, Hiroyuki Iwahana, Shiro Sasaki, Seiji Arase and Mitsuo Itakura : p53 Gene Mutation Analysis in Porokeratosis and Porokeratosis-associated Squamous Cell Carcinoma., Journal of Dermatological Science, Vol.14, No.3, 173-178, 1997.
(Summary)
In this and previous studies, we have shown p53 overexpression immunohistochemically in 14 of 17 porokeratotic specimens obtained from 14 lesions of nine cases, and in all six specimens of squamous cell carcinoma (SCC) arising on porokeratotic lesions of two cases. We screened mutations in exons 5 to 10 of the p53 gene in all these specimens by polymerase chain reaction-single strand conformation polymorphism analysis. Mutations of the p53 gene were detected in two of the six SCCs but not in any of the 17 porokeratotic specimens. These two mutations were C to T transitions at codons 146 and 175 in exon 5, which were a nonsense mutation at a dipyrimidine site and a missense mutation at a CG site, respectively. To our knowledge, neither of these mutations has been identified in skin cancers before. Our observations indicate that mutations of the p53 gene are not the major molecular etiology for porokeratosis, but are related to its skin carcinogenesis, and that p53 overexpression in porokeratosis is not due to p53 gene mutations.
Y Miyaoka, Y Urano, Y Nameda, K Shigekiyo, T Horie, Nobuya Sano and Seiji Arase : A Case of Dermatomyositis Complicated by Thrombotic Thrombocytopenic Purpura, Dermatology, Vol.194, 68-71, 1997.
S. Kato, Y. Urano, S. Sasai, K. Ahsan, Masayuki Shono and Seiji Arase : Prolonged nuclear accumulation of p35 in xeroderma pigmentosum complementation group A cell after ultraviolet irradiation, Arch Dermatol Res., Vol.288, 249-251, 1996.
(Keyword)
nuclear accumulation / ultraviolet irradiation
61.
S. Sasaki, Y. Urano, K. Nakagawa, Hiroaki Nagae, Hideki Nakanishi and Seiji Arase : Linear porokeratosis with multiple squamous cell carcinomas Study of p53 expression in porokeratosis and squamous cell carcinoma, The British Journal of Dermatology, Vol.134, No.6, 1151-1152, 1996.
Kazuya Matsumoto, 浦野 芳夫, Hideki Nakanishi, 井上 利之, 内田 尚之, Seiji Arase and Yuichi Fujinaka : 悪性リンパ腫を合併した基底細胞癌の2例, Japanese Journal of Plastic Surgery, Vol.39, No.9, 911-916, 1996.
64.
宮本 洋, Hideki Nakanishi, Hiroaki Nagae, Masahiro Yamano, 田中 伸二, Ichiro Hashimoto, Kazuya Matsumoto and Seiji Arase : 3世代にわたって発症した軸前性多合趾症の1例, Japanese Journal of Plastic Surgery, Vol.39, No.2, 205-210, 1996.
65.
Ichiro Hashimoto, Hideki Nakanishi, 加藤 昭二, Hiroaki Nagae, Takuya Seike, Seiji Arase and 近藤 和也 : 骨外性粘液型軟骨肉腫に対する遊離植皮術後に発症した肺塞栓の1例, Japanese Journal of Plastic Surgery, Vol.39, No.2, 197-203, 1996.
66.
宮本 洋, Hideki Nakanishi, 長江 浩朗, 浦野 芳夫 and Seiji Arase : 7%リドカインクリームと60%リドカインテープの効果比較―リドカインクリームの採皮における使用経験も含めて―, Japanese Journal of Plastic Surgery, Vol.39, No.9, 883-887, 1996.
67.
宮本 洋, Hideki Nakanishi, Kazuya Matsumoto, 浦野 芳夫 and Seiji Arase : 巨大な石灰化上皮腫, Hifuka No Rinsho, Vol.38, No.7, 1154-1155, 1996.
68.
H. Nagae, Hideki Nakanishi, Y. Urano and Seiji Arase : Serial Cultivation of Human Nail Matrix Cells under Serum-free Conditions, The Journal of Dermatology, Vol.22, No.8, 560-566, 1995.
(Summary)
We have established serial cultures of human nail matrix cells (NMCs) under serum-free conditions. We cultured NMCs using two different methods depending upon the volume of nail matrix obtained. When a sufficient amount of nail matrix was obtained, they were minced and treated with 0.25% trypsin and 0.03% EDTA. The NMCs were transferred directly as a dispersed cell culture into KGM medium. Because a sufficient amount of matrix was rarely obtained, we developed a method by which NMCs were cultured primarily as implanted small matrices in Eagle's MEM (high Ca+ medium) supplemented with 15% fetal bovine serum for the first 4 to 5 days; during this time, the NMCs expanded from the matrices and formed colonies around them. NMCs then were cultured with KGM. In both methods, KGM medium supported the growth of NMCs without a biological feeder layer. These cells could be cultivated serially for at least seven passages. Half of the cells were positively stained with a monoclonal antibody against hair (hard) keratin which is expressed in nail matrix in vivo, indicating that the cells originated from the nail matrix. These methods will now permit investigations of nail matrix cells that previously were unfeasible because of the relative lack of cells and difficulties with propagation.
Takuya Seike, Hideki Nakanishi, Yoshio Urano and Seiji Arase : Malignant Melanoma Developing in an Area of Palmoplantar Keratoderma (Greithers Disease), The Journal of Dermatology, Vol.22, No.1, 55-61, 1995.
Ichiro Hashimoto, Hideki Nakanishi and Seiji Arase : Effect of deferoxamine on thrombusm formation in a Rabbit Ear Chamber of an ischemic flap, Scientific papers of x I th international conferderation for Plastic Surgery, 95-96, 1995.
73.
Ichiro Hashimoto, Hideki Nakanishi, Seiji Arase and 佐野 寿昭 : Merkel Cell Carcinomaの1例, Hifuka No Rinsho, Vol.37, No.4, 642-643, 1995.
74.
Yoshiaki Kubo, Yoshio Urano, Katsuhiko Yoshimoto, Hiroyuki Iwahana, Kosaku Fukuhara, Seiji Arase and Mitsuo Itakura : p53 Gene Mutations in Human Skin Cancers and Precancerous Lesions: Comparison with Immunohistochemical Analysis., The Journal of Investigative Dermatology, Vol.102, No.4, 440-444, 1994.
75.
庄野 佳孝, Hideki Nakanishi, Hiroaki Nagae, 山本 忠利 and Seiji Arase : 第1鰓裂由来の瘻孔および嚢腫の3例, Japanese Journal of Plastic Surgery, Vol.37, No.11, 1291-1297, 1994.
76.
武田 克之, Hideki Nakanishi, Seiji Arase, 田中 伸二 and 庄野 佳孝 : 皮膚潰瘍に対するSNK-863軟膏の臨床評価, Japanese Journal of Medicine and Pharmaceutical Science, Vol.32, No.4, 755-768, 1994.
Y. Urano, Hajimu Oura, A. Sakaki, Hiroaki Nagae, Kazuya Matsumoto, K. Fukuhara, T. Nagae, Seiji Arase, Y. Ninomiya, Hideki Nakanishi, F. Shigemi and K. Takeda : Immunohistological analysis of P53 expression in human skin tumors, Journal of Dermatological Science, Vol.4, No.2, 69-75, 1992.
(Summary)
The p53 expression in various skin tumors was immunohistologically evaluated using two mouse monoclonal anti-p53 antibodies, PAb421 and PAb1801. The p53 expression was not detected in the normal epidermal cells. Nuclear staining suggested that the p53 expression was observed in 10 of 26 squamous cell carcinomas (SCCs) from 24 patients, in one undifferentiated carcinoma, one proliferating trichilemmal cyst, one malignant proliferating trichilemmal tumor and in one metastatic carcinoma of breast cancer. None off four cases of Bowen's disease (SCC in situ) showed nuclear staining. In the SCCs, five of 20 primary lesions, three of four recurrent lesions and both of two metastatic lesions had positive nuclei. There was one case of SCC in which a primary lesion was negative but a recurrent lesion was positive. Thus, p53 expression was more frequently observed in SCCs at more clinically advanced stages. This may suggest that p53 has some relevance to progression of SCC. Nuclear staining was not detected in any of the following cases: two cases of seborrheic keratosis, one eccrine poroma, one keratoacanthoma, 11 basal cell epitheliomas, two mammary Paget's disease, three genital Paget's disease, one sebaceous carcinoma, four malignant melanomas, six lymphomas, two leukemia cutis and two angiosarcomas.
Hideki Nakanishi, Seiji Arase, Ichiro Hashimoto and Shinji Tanaka : VASODILATORY EFFECTS OF PROSTAGLANDIN E1(PGE1)ON MICROCIRCULATION OBSERVED IN THE RABBIT EAR CHAMBER, International confederation for plastic and reconstructive surgery, Vol.2, 9-10, 1992.
89.
Hirotsugu Takiwaki, Hideki Nakanishi, Y. Shono and Seiji Arase : The influence of cutaneous factors on the transcutaneous PO2 and PCO2 at various body sites, The British Journal of Dermatology, Vol.125, No.3, 243-247, 1991.
(Summary)
The transcutaneous partial pressures of oxygen (tcpO2) and carbon dioxide (tcpCO2) were measured at eight different sites in 10 adult male subjects with an electrode at a temperature of 44 degrees C. The mean tcpO2 values (mmHg) were significantly lower on the face (forehead 26.6, cheek 29.6) and the palm (26.4) than at other sites (60.6-69.6). The tcpCO2 values (mmHg) were only slightly higher on the face. Removal of the stratum corneum produced an average increase of the tcpO2 on the palm of 37.6 mmHg and on the forehead of 19.6 mmHg. However, in 10 children with an age range of 3-9 years, the difference in the mean pO2 between the cheek and forearm was very small. There was no significant difference in the cutaneous blood flow at 44 degrees C between the cheek, palm and forearm.
(Keyword)
Adult / aging / Blood Gas Monitoring, Transcutaneous / carbon dioxide / Cheek / children / Child, Preschool / Forearm / Hand / Humans / Male / Oxygen / Partial Pressure / Regional Blood Flow / Skin
(Link to Search Site for Scientific Articles)
● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 1911316
Seiji Arase, Y. Sadamoto, R. Kuwana, Hideki Nakanishi, K. Fujie, K. Takeda and E. Takeda : The effect of 1, 25-dihydroxyvitamin D3 on the growth and differentiation of cultured human outer root sheath cells from normal subjects and patients with Vitamin D-dependent rickets type with alopecia, Journal of Dermatological Science, Vol.2, No.5, 353-360, 1991.
(Summary)
We examined the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on the growth and differentiation of cultured human hair outer root sheath cells (ORSC) from normal subjects and patients with vitamin D-dependent rickets type II (DDR-II) with alopecia. 1,25(OH)2D3 dose-dependently suppressed the plating efficiency, clonal growth, and DNA synthesis of normal ORSC. It enhanced the cornified envelope formation and caused morphological changes in the cells. All results indicated the existence of specific receptors for 1,25(OH)2D3 in the ORSC, and suggest that 1,25(OH)2D3 is a potent inhibitor of proliferation of ORSC as well as a stimulator of terminal differentiation. However, the cells from DDR-II patients with alopecia did not respond to 1,25(OH)2D3, suggesting a lack of the specific receptors in the cells. The differences in the cellular response to the hormone between the normal ORSC and those from the patients were apparent and easily distinguishable, therefore this experiment may be a rapid and simple diagnostic test for DDR-II patients with alopecia. Large number of hairs were difficult to obtain from patients with alopecia, and we developed a new culture method to accomplish these studies from a few plucked hair follicles. Our system may be useful in the culture of ORSC from limited number of follicles, and could be utilized to analyse the cellular characteristics of ORSC in patients with hair diseases.
Hirotsugu Takiwaki, Seiji Arase, Hideki Nakanishi and K. Takeda : Transcutaneous PO2 and PCO2 Measurements in Various Skin Lesions, The Journal of Dermatology, Vol.18, No.6, 311-313, 1991.
(Summary)
Low tcPO2 levels were observed in various skin lesions, indicating that most pathological changes in the skin induce such a reduction. In contrast, the tcPCO2 changed less, with the exception of marked elevations in bullous or prenecrotic lesions. This procedure may have practical applications in clinical dermatology, particularly in quantitating the response to therapy and in predicting a necrotic change.
Seiji Arase, Shoji Katoh, Yasushi Sadamoto, Hideki Nakanishi, Yoshio Urano, Kenji Fujie and Katsuyuki Takeda : Culture of human outer root sheath cells from plucked hair follicles in serum-free conditions, Journal of Dermatological Science, Vol.2, No.1, 66-70, 1991.
(Summary)
We succeeded in culturing human outer root sheath cells (ORSC) in serum-free conditions without a biological feeder layer. The combination of collagen type IV substrate and modified MCDB 153 medium supplemented with bovine pituitary gland extract (K-GM medium) could support the growth of ORSC. These cells can be serially cultivated for at least 4 passages and stored in liquid nitrogen with good recovery. Thus, a large series of experiments using ORSC may be run simultaneously.
(Keyword)
cell division / Cells, Cultured / collagen / Colony-Forming Units Assay / Culture Media / Hair / Humans
(Link to Search Site for Scientific Articles)
● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 2054340
原田 伸, Hideki Nakanishi, Hiroaki Nagae, Hirotsugu Takiwaki, Seiji Arase and 福田 善晴 : クレゾールによる自傷性皮膚腫瘍を呈したMünchausen症候群の1例, Japanese Journal of Plastic Surgery, Vol.34, No.8, 839-845, 1991.
95.
戸田 則之, Hideki Nakanishi, Hiroaki Nagae, 原田 伸, Seiji Arase, 重見 文雄 and 武田 克之 : 唇・顎裂を合併した高度短合指症例の経験, Japanese Journal of Plastic Surgery, Vol.34, No.7, 727-731, 1991.
96.
Hideki Nakanishi, Hiroaki Nagae, 原田 伸, 戸田 則之, Seiji Arase and 武田 克之 : Treacher collins症候群の治療経験 ―側頭筋頭蓋骨移植―, Japanese Journal of Plastic Surgery, Vol.34, No.6, 631-636, 1991.
97.
Kazuya Matsumoto, Hideki Nakanishi, Hiroaki Nagae, 田中 伸二, 中川 宏治, Ichiro Hashimoto and Seiji Arase : Malignant proliferating trichilemmal tumorの2例, Japanese Journal of Plastic Surgery, Vol.34, No.4, 377-382, 1991.
98.
Hideki Nakanishi, Hiroaki Nagae, 戸田 則之, Seiji Arase and 武田 克之 : 外陰癌の治療体験, Japanese Journal of Plastic Surgery, Vol.34, No.2, 157-163, 1991.
99.
Hideki Nakanishi, 原田 伸, Seiji Arase and 武田 克之 : 卵黄腸管(臍腸管)遺残の2例 ―臍形成について―, Japanese Journal of Plastic Surgery, Vol.34, No.1, 79-84, 1991.
100.
Hirotsugu Takiwaki, Seiji Arase and Hideki Nakanishi : 外傷性脂肪壊死の3例, Japanese Journal of Clinical Dermatology, Vol.44, No.3, 241-244, 1990.
101.
Seiji Arase, S. Kato, Hideki Nakanishi, Y. Sadamoto, Y. Urano and K. Takeda : Eosinophilic Polymyositis Induced by Tranilast, The Journal of Dermatology, Vol.17, No.3, 182-186, 1990.
(Summary)
A 38-year-old man with asthma developed eosinophilic polymyositis following the administration of Tranilast, an antiasthmatic agent. Low grade fever, erythematous rashes on the entire body, dysphagia, blood eosinophilia, elevations of serum creatine phosphokinase and myoglobin levels, and inverted T waves in the electrocardiogram were noted. A muscle biopsy showed focal degeneration of muscle fibers with an infiltrate of eosinophils and lymphocytes. A rechallenge with Tranilast resulted in erythema formation, blood eosinophilia, and elevations of some serum muscle enzymes and myoglobin levels. Tranilast was considered to be the causative agent. This is the first reported case of Tranilast-induced eosinophilic polymyositis.
(Keyword)
Asthma / Drug Eruptions / Eosinophilia / Humans / Male / Middle Aged / Myositis / ortho-Aminobenzoates
(Link to Search Site for Scientific Articles)
● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 1693928
Hiroaki Nagae, Hideki Nakanishi, 原田 伸, 戸田 則之, 田中 伸二, Seiji Arase and 武田 克之 : 外傷後に生じた鼻部囊腫の1例, Japanese Journal of Plastic Surgery, Vol.33, No.8, 773-776, 1990.
104.
戸田 則之, Hideki Nakanishi, Hiroaki Nagae, 田中 伸二, 菊池 誠, Seiji Arase, 重見 文雄 and 武田 克之 : A case of postoperative maxillary cyst., The Nishinihon Journal of Dermatology, Vol.52, No.2, 263-267, 1990.
(Summary)
A 33-year-old man, who had received an operation for paranasal sinusitis at the age 16, developed an asymptomatic mass in the right cheek. Our examination showed a cyst destroying the maxillary bone. The cyst was surgically removed and ultimately considered to be a postoperative maxillary cyst. The resulting defect in the maxillary bone was filled with iliac bone chips with satisfactory results. Though most cases of such cysts tend to occur under the supraorbital nerve and vessels, this case was found to overlie them. We reviewed previously reported cases and briefly discussed the cause, differential diagnosis, and treatment of this disorder.
Seiji Arase, Hideki Nakanishi, 武田 克之 and 藤本 篤夫 : Mycobacterium fortuitum皮膚感染症, The Nishinihon Journal of Dermatology, Vol.52, No.2, 241-244, 1990.
106.
定本 靖司, Seiji Arase, 加藤 昭二, 藤江 建志, Hideki Nakanishi and 武田 克之 : ビタミンD依存性クル病Ⅱ型患者毛包細胞に及ぼす1，25-dihydroxyvitamin D3の影響, The Japanese Journal of Dermatology, Vol.100, No.2, 211-214, 1990.
107.
Seiji Arase and Hideki Nakanishi : Liquid-holding recovery of sister chromatid exchanges in UV-irradiated normal and xeroderma pigmentosum complementation groups A and F fibroblasts., Archives of Dermatological Research, Vol.282, 68-70, 1990.
108.
Seiji Arase, R Kuwana, Y Sadamoto, Hideki Nakanishi, F Shigemi and K Takeda : CULTURE OF DISPERSED HAIR FOLLICLE CELLS FROM PLUCKED OUT HAIRS WITHOUT A FEEDER LAYER, The Tokushima Journal of Experimental Medicine, Vol.36, No.3,4, 87-95, 1989.
(Summary)
Dispersed hair follicle cells from plucked out hairs have been successfully grown on collagen type IV without a biological feeder layer. The optimal calcium concentration for the culture of these cells was also studied and decided to be 0.3-0.5 mM. In this calcium range and on collagen type IV, colony formation and colony growth were best and big colonies with a paving stone-like cell arrangement were formed. The availability of such cultured cells for the treatment of missing skin is proposed instead of cultured epidermal keratinocytes which have been commonly used for wound dressing.
Seiji Arase, Hideki Nakanishi, 原田 伸, 重見 文雄 and 武田 克之 : Follicular atrophoderma with multiple basal cell carcinomas. An abortive form of bazex syndrome.:-An Abortive Form of Bazex Syndrome-, The Nishinihon Journal of Dermatology, Vol.51, No.2, 250-254, 1989.
(Summary)
Multiple basal cell carcinomas developed predominantly on the sun exposed areas in a 52-year-old Japanese man. He also had follicular atrophoderma on the extensor aspect of the extremities. Five other siblings were free from symptoms, but his mother suffered from follicular atrophoderma. These findings were consistent with the syndrome described by Bazex et al. However, because the patient lacked other cardinal skin synptoms such as abnormalities of scalp hair and sweating, a diagnosis of an abortive form of Bazex syndrome was made. Numerous basal cell carcinomas were also observed on the trunk, a non-exposed area, previously irradiated with X-rays. We would like to emphasize that radiation therapy should be avoided in patients with Bazex syndrome. Here we report the first case of this rare inherited syndrome in Japan.
Hiroaki Nagae, Hideki Nakanishi, 原田 伸, 戸田 則之, Seiji Arase and 武田 克之 : 先天性上口唇正中瘻孔の1例, Japanese Journal of Plastic Surgery, Vol.32, No.2, 187-189, 1989.
115.
桑名 隆一郎, Seiji Arase, 定本 靖司, 神野 公孝, Hideki Nakanishi and 武田 克之 : Postconfluent Hair Follicle Cellsに対するMinoxidilの影響, The Nishinihon Journal of Dermatology, Vol.51, No.1, 66-70, 1989.
116.
定本 靖司, Seiji Arase, 桑名 隆一郎, Hideki Nakanishi and 武田 克之 : 1.25-Dihydroxyvitamin D3のHair Follicle Cellに与える影響, The Nishinihon Journal of Dermatology, Vol.51, No.1, 59-65, 1989.
117.
桑名 隆一郎, Seiji Arase, 定本 靖司, Hideki Nakanishi and 武田 克之 : Effect of Minoxidil on Cultured Human Hair Follicle Cells, The Nishinihon Journal of Dermatology, Vol.50, No.5, 879-881, 1988.
(Summary)
Minoxidil (MX), whether applied topically or taken orally, has been said to increase the thickness and length of hairs. Knowing the functions of MX on the scalp hairs, we investigated the effect of MX on preconfluent human hair follicle cells in culture. The hair follicle cells used were derived from plucked scalp hair follicles according to the culture methods previously reported by us. Both plating efficiency and colony growth were supressed by MX at all the drug concentrations tested: 1, 10 and 100 <I>μ</I>g/ml. Cell morphology was not so affected by MX. Terminal differentiations such as stratification of cells and appearance of large denucleated cells were seen at all MX levels, as in the control. We found no evidence that MX activates the proliferation, or modulates the differentiation of HFC in vitro.
Seiji Arase, Hideki Nakanishi, 藤本 篤夫, Hiroaki Nagae, 重見 文雄 and 武田 克之 : Xeroderma Pigmentosum with Diabetes Mellitus.:-A Report of 2 Cases plus Clinical and Biological Studies of 8 Cases of Xeroderma Pigmentosum Occurring in Tokushima-, The Nishinihon Journal of Dermatology, Vol.50, No.5, 833-838, 1988.
(Summary)
Two brothers with XP were found to be suffering from diabetes mellitus. The elder brother also had psoriasis vulgaris. XP is an autosomal recessive disease with an extensive heterogeneity in clinical features, and is characterized by cellular DNA repair deficiency. On the other hand, both ataxia telangiectasia and Fanconi's anemia are also autosomal recessive syndromes characterized by certain DNA repair defects. And it has been well known that patients (homozygotes) with these syndromes frequently develop diabetes mellitus. These observations led us to suppose that XP patients may also be predisposed to DM. Clinical and biological studies of 8 patients with XP in Tokushima have been carried out. Three patients of rather an old age (older than 48) had diabetes mellitus, and one had a hyperlipemia. The number of patients analyzed was very small, but it should be noted that the frequency of DM in XP patients might be high. We also report the first case of XP occurring together with DM and psoriasis vulgaris in Japan.
Seiji Arase, 定本 靖司, 桑名 隆一郎, 渡部 泰守, Hideki Nakanishi, 重見 文雄 and 武田 克之 : 脱毛を合併するビタミンD依存性クル病Ⅱ型の5症例, The Japanese Journal of Dermatology, Vol.98, No.10, 1029-1038, 1988.
(Summary)
紫外線照射により皮膚で合成されたり，または食物として摂取されたビタミンDは，肝臓と腎臓で水酸化を受け，ビタミンD作用(カルシウム代謝の調節など)を実際に発揮する1.25-dihydroxyvitaminD3(1.25(OH)2D3と略記)に変換される．1.25(OH)2D3は標的臓器細胞中の特異レセプターと結合し，DNAに働き，蛋白合成を介してホルモン効果を発揮する．この一連の機序のどこかに障害があるとカルシウム代謝異常症が出現する．1978年，Brooksらは，1.25(OH)2D3の合成，代謝は正常であるが，それに対する標的細胞内のレセプター異常の結果発症するクル病をビタミンD依存性クル病Ⅱ型(Vitamin D Dependent Reckets type II，以下DDR2と略記)として報告した．以後30例近く報告されているが，注目すべきことに大半の患者に脱毛がみられる．本邦でも6症例の報告を見るが，いずれも小児科，内科医により発表され，皮膚医にとってなじみの少ない疾患と思われる．しかし乳幼児，小児における脱毛の診察にあたっては，鑑別疾患の1つにあげねばなるまい．今回我々は脱毛を伴うDDR2の5症例を経験したので，主に脱毛についての考察を加えて報告する．
Hideki Nakanishi, Seiji Arase, 戸田 則之 and 武田 克之 : Duo DERMの採皮創に対する臨床経験 ―細菌検査を中心に―, 臨床医薬, Vol.4, No.8, 1483-1486, 1988.
121.
桑名 隆一郎, Seiji Arase, 定本 靖司, Hideki Nakanishi and 武田 克之 : A Method for Culturing Human Hair Follicle Cells (III):-Determination of optimal culture conditions: The effects of substrates and Ca<SUP>++</SUP> concentrations-, The Nishinihon Journal of Dermatology, Vol.50, No.3, 432-438, 1988.
(Summary)
The effects of substrates and medium ionic calcium concentrations on the growth and differentiation of human hair follicle cells in dispersed cell culture were investigated. Compared to other extracellular matrices such as collagen type I, fibronectin and laminin, a substrate of collagen type IV increased colony formation 3-7 fold. Colony growth of human hair follicle cells was doubled at medium ionic calcium concentrations of 0.3 mM compared to higher or lower levels, and terminal differentiation of human hair follicle cells was suppressed with lower calcium levels over the range 0.1∼0.9 mM.
桑名 隆一郎, Seiji Arase, 定本 靖司, Hideki Nakanishi and 武田 克之 : A Method for Culturing Human Hair Follicle Cells (II):-Plucked Human Hair Follicles-, The Nishinihon Journal of Dermatology, Vol.50, No.2, 271-275, 1988.
(Summary)
We performed an explant culture and a dispersed cell culture of plucked hair follicles and could obtain hair follicle cell colonies without contamination of fibroblasts by both culture methods. To determine the optimal culture condition, MMC treated 3T3 cells, collagen type I membrane (undcr surface: 3T3 cells without MMC treatment) and collagen type IV were used as feeder layers or substrate. The hair follicle cell growth could be constantly viewed only on the collagen type IV coated dishes. Therefore, it is suggested that collagen type IV, a component of the basement membrane, is essential for the growth of hair follicle cells from plucked human hair follicles.
桑名 隆一郎, Seiji Arase, 定本 靖司, Hideki Nakanishi, 榊 哲彦 and 武田 克之 : A Method for Culturing Human Hair Follicle Cells (I):-A Method for Culturing Hair Follicles Obtained from Surgically Resected Scalp-, The Nishinihon Journal of Dermatology, Vol.50, No.1, 97-101, 1988.
(Summary)
We cultured human hair follicle cells from follicles of the surgically resected scalp skin. In the presence of calcium (1.96 mM), a physiological concentration, the cells piled up, keratinized and rapidly displayed a terminal differentiation, as seen in skin epidermal cells. Only when they were cultured from the start with a physiological calcium concentration medium and then with a low calcium concentration one (0.43 mM) did monolayered, polygonal and large nucleated basal cell-like cells appear, and were retained for about 20 days. To remove the fibroblasts which grow rapidly and surround hair follicle cell colonies in the course of culture, we scraped them off with a rubber policeman and the growth was suppressed to some extent.
Seiji Arase, R. Nagai, Hideki Nakanishi and Katsuyuki. Takeda : EFFECTS OF SIX TOPLCAL CORTLCOSTEROIDS ON DNA SYNTHESIS OF NORMAL HUMAN SKIN FIBROBLASTS, The Tokushima Journal of Experimental Medicine, Vol.35, No.1-2, 1-4, 1988.
Seiji Arase, Hideki Nakanishi, 永井 隆 and 重見 文雄 : フトラフール誘発ループス-Wide Spread DLE, Hifuka No Rinsho, Vol.29, No.10, 951-957, 1987.
128.
戸田 則之, Seiji Arase, Hirotsugu Takiwaki, Hideki Nakanishi, 白神 あきら and 高橋 秀夫 : 慢性リンパ球性白血病患者に合併したNecrotizing Fasciitisの1例, Japanese Journal of Clinical Dermatology, Vol.41, No.8, 575-578, 1987.
129.
Seiji Arase, Hideki Nakanishi, 永井 隆, 武田 克之 and 居村 洋 : Treatment of Patients with Darier's Disease with Topical Application of Solbase (a Mixture of Polyethylene Glycol 400 & 4000) Plus Occlusion, The Nishinihon Journal of Dermatology, Vol.49, No.2, 290-295, 1987.
(Summary)
Topical application of Solbase (a mixture of polyethylene glycol 400 & 4000) plus occlusion improved the eruptions of 2 patients with Darier's disease. To determine the mechanism of action, the effect of polyethelene glycol 400 on the rat epidermal DNA synthesis was investigated using autoradiography and doing <SUP>14</SUP>C-thymidine incorporation studies. Seven days' topical application of polyethylene glycol 400 activated epidermal DNA synthesis nearly double the value seen in the control. This therapy should be considered for patients with Darier's disease and especially for whom retinoids cannot be prescribed.
Ichiro Hashimoto, Hideki Nakanishi, Yoshitaka Shono, Maki Toda, Hidetaka Tsuda and Seiji Arase : Angiostatic effects of corticosteroid on wound healing of rabbit ear, The Journal of Medical Investigation : JMI, Vol.49, No.1,2, 61-66, 2002.
(Summary)
Wound healing is a complex biologic process with initial inflammation, granulation tissue formation, and matrix remodeling. We observed the relation between angiostatic effects and corticosteroid administration time in the rabbit ear chamber. Angiogenesis in the chamber was studied using a microscope-television system. Two experiments were undertaken to represent the systemic and the topical administration of steroids. In experiment 1, 10 mg of triamcinolone acetonide was injected three times intramuscularly (on the day of implantation of the chamber, and the 7th and 14th day after implantation). Vascularization in this group was significantly delayed at the 7th, 14th, and 21st days but no difference from controls was observed in the size and density of vessels after its completion. In experiment 2, 3 mg of triamcinolone acetonide was injected once into the skin adjacent to the chamber on the 10th day after installment of chambers or on the day of installment. In the former group, new vascular growth was delayed until the 21st day after installment. The hemorrhagic zone had narrowed and vascular dilation was observed. In the latter group, endothelial budding was delayed and vascular constriction occurred. New vascular growth was severely delayed and granulation filling of the chamber was not completed. These results suggest not only that the topical administration had the stronger inhibitory effect on neovascularization than the systemic administration but that the effect differed depending on the stage of wound healing. In view of this effect of this steroid, we should pay careful attention to the time when steroids are administered to patients.
Kazutoshi Murao, Yoshiaki Kubo and Seiji Arase : Woolly hair nevus: neonatal alopecia can be an initial manifestation., International Journal of Dermatology, Vol.52, No.12, 1588-1589, 2013.
Kazutoshi Murao, Yoshiaki Kubo, Takeshi Ishigami and Seiji Arase : Lack of association between extramammary Pagets disease and human papillomavirus infection, Journal of Dermatological Science, Vol.59, No.3, 209-210, 2010.
(Keyword)
Aged / Aged, 80 and over / Female / Humans / Male / Paget Disease, Extramammary / Papillomavirus Infections / Skin Neoplasms
Kazutoshi Murao, H Takeichi, Yoshiaki Kubo and Seiji Arase : Multiple pilocebaseouc systs induced by topical steroid therapy, The 1st Eastern Asia Dermatology Congress, Sep. 2010.
2.
Ichiro Hashimoto, Hideki Nakanishi and Seiji Arase : Effect of Deferoxamine on Thrombus Formation in a Rabbit Ear Chamber of an Ischemic Flap, 11TH CONGRESS OF THE INTERNATIONAL CONFEDERATION FOR PLASTIC,RECONSTRUCTIVE AND AESTHETIC SURGERY, Yokohama, Apr. 1995.
3.
Hideki Nakanishi, Seiji Arase, Ichiro Hashimoto and Shinji Tanaka : VASODILATORY EFFECTS OF PROSTAGLANDIN E1(PGE1) ON MICROCIRCULATION OBSERVED IN THE RABBIT EAR CHAMBER, X CONGRESS OF THE INTERNATIONAL CONFEDERATION FOR PLASTIC AND RECONSTRUCTIVE SURGERY, SPAIN, Jun. 1992.