Tokushima University / Educator and Researcher Directory --- Miyake, Yoichiro

Personal Web Page

https://researchmap.jp/read0184039 (researchmap)

Field of Study

○	Oral Microbiology
○	Oral Microbiology
○	Antibacterial Chemotherapy
○	Microbiology

Subject of Study

○	Bacterial Adherence (microorganism, Adherence)
○	Prevention of Aspiration Pneumonia (Aspiration Pneumonia, prevention)
○	Tolerance to Antibacterial Agents (Antibacterial Agents, Tolerance)
○	Bacterial Stress Response (stress response, bacteria)

Book / Paper

Book:

1.	Hiromichi Yumoto, Katsuhiko Hirota, Kouji Hirao, Masami Ninomiya, Keiji Murakami, Hideki Fujii and Yoichiro Miyake : The Pathogenic Factors from Oral Streptococci for Systemic Diseases, Aug. 2020.
2.	Yoichiro Miyake : 9. 口腔微生物叢の理解, Ishiyaku Publishers,Inc., Tokyo, May 2016.
3.	Yoichiro Miyake : 歯科衛生士のための口腔機能管理マニュアル, Ishiyaku Publishers,Inc., Tokyo, May 2015.
4.	Ishikawa Akira, Yoichiro Miyake, Yoneyama Takeyoshi and Miyatake Kokichi : Prevention of aspiration pneumonia in the elderly by reducing oropharyngeal microflora, Nova Science Publishers, Sep. 2009.
5.	Akira Ishikawa, Yoichiro Miyake, Takeyoshi Yoneyama and Kokichi Miyatake : Chapter15; Prevention of Aspiration Pneumonia in the Elderly by Reducing Oropharyngeal Microflora, Nova Science Publishers, Inc., Sep. 2009.
6.	梅本俊夫, 奥田克爾, 中山浩次 and Yoichiro Miyake : 新歯科衛生士教本微生物学第2版, Ishiyaku Publishers,Inc., Tokyo, Mar. 2007.
7.	Toshiya Kashiwabara, Tetsuo Ichikawa, Naeko Kawamoto, Hideo Kanitani, Masanobu Horiuchi, Katsuhiko Hirota, Yoichiro Miyake and Naoyuki Matsumoto : EBMに基づいた口腔ケアのために必読文献集(日本歯科医師会監修/静岡県歯科医師会編), Ishiyaku Publishers,Inc., Tokyo, Mar. 2002.

Academic Paper (Judged Full Paper):

1.	Akihiro Wada, Keiji Murakami, Yumi Ishikawa, Takashi Amoh, Kouji Hirao, Yuki Hosokawa, Daisuke Hinode, Yoichiro Miyake and Hiromichi Yumoto : Anti-Inflammatory and Protective Effects of Juncus effusus L. Water Extract on Oral Keratinocytes, BioMed Research International, Vol.2022, 9770899, 2022. (Summary) Periodontitis is a chronic inflammatory disease caused by periodontopathogenic bacteria that form biofilms in periodontal pockets. The gingival epithelium acts as the first physical barrier in fighting attacks by periodontopathogenic pathogens, such as the primary etiological agent , and various exogenous chemicals, as well as regulates the local innate immune responses. Therefore, the development of novel oral care products to inhibit inflammatory reactions caused by bacterial infection and protect the gingival epithelium is necessary. L. has generally been used as an indigenous medicine, such as a diuretic, an antipyretic, and an analgesic, in ancient practice. In this study, we examined the effects of a water extract from L. on the inhibition of the inflammatory reaction elicited by bacterial infection and protection of the oral epithelium by chemical irritation. Pretreatment of oral epithelial cells with the water extract from L. significantly reduced or its lipopolysaccharide- (LPS-) mediated production of chemokines (interleukin-8 and C-C-chemokine ligand20) in a concentration-dependent manner with comparable to or greater effects than epigallocatechin gallate and protected oral epithelial cells from injury by chemical irritants, cetylpyridinium chloride, and benzethonium chloride. Moreover, the water extract from . L. in the presence of antimicrobial agents or antifibrinolytics already used as ingredients in mouthwash could significantly reduce the production of chemokines from LPS-stimulated oral epithelial cells in a concentration-dependent manner. These findings suggest that the water extract from . L. is potentially useful for oral care to prevent oral infections, such as periodontal infections, and maintain oral epithelial function. (Keyword) Anti-Inflammatory Agents / Bacteroidaceae Infections / Cell Line, Transformed / Humans / Keratinocytes / Magnoliopsida / Mouth Mucosa / Periodontitis / Plant Extracts / Porphyromonas gingivalis (Tokushima University Institutional Repository) ● Metadata: 117031 (Link to Publication Site) ● Publication site (DOI): 10.1155/2022/9770899 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35028318 ● Summary page in Scopus @ Elsevier: 2-s2.0-85123567270 (Tokushima University Institutional Repository: 117031, DOI: 10.1155/2022/9770899, PubMed: 35028318, Elsevier: Scopus)
2.	Momoyo Azuma, Keiji Murakami, Rina Murata, Keiko Kataoka, Hideki Fujii, Yoichiro Miyake and Yasuhiko Nishioka : Clinical Significance of Carbapenem-Tolerant Pseudomonas aeruginosa Isolated in the Respiratory Tract, Antibiotics, Vol.9, No.9, 2020. (Summary) infections. (Tokushima University Institutional Repository) ● Metadata: 116480 (Link to Publication Site) ● Publication site (DOI): 10.3390/antibiotics9090626 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32967210 ● Search Scopus @ Elsevier (PMID): 32967210 ● Search Scopus @ Elsevier (DOI): 10.3390/antibiotics9090626 (Tokushima University Institutional Repository: 116480, DOI: 10.3390/antibiotics9090626, PubMed: 32967210)
3.	Natsumi Fujiwara, Keiji Murakami, Kaya Yoshida, Shunsuke Sakurai, Yasusei Kudo, Kazumi Ozaki, Katsuhiko Hirota, Hideki Fujii, Maiko Suzuki, Yoichiro Miyake and Hiromichi Yumoto : Suppresive effects of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer on the adherence of Candida species and MRSA to acrylic denture resin., Heliyon, Vol.6, e04211, 2020. (Tokushima University Institutional Repository) ● Metadata: 115916 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.heliyon.2020.e04211 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32577575 ● Summary page in Scopus @ Elsevier: 2-s2.0-85086425832 (Tokushima University Institutional Repository: 115916, DOI: 10.1016/j.heliyon.2020.e04211, PubMed: 32577575, Elsevier: Scopus)
4.	Natsumi Fujiwara, Hiromichi Yumoto, Koji Miyamoto, Katsuhiko Hirota, Hiromi Nakae, Saya Tanaka, Keiji Murakami, Yasusei Kudo, Kazumi Ozaki and Yoichiro Miyake : 2-Methacryloyloxyethyl phosphorylcholine (MPC)-polymer suppresses an increase of oral bacteria: a single-blind, crossover clinical trial, Clinical Oral Investigations, Vol.23, 739-746, 2019. (Summary) The biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymers, which mimic a biomembrane, reduce protein adsorption and bacterial adhesion and inhibit cell attachment. The aim of this study is to clarify whether MPC-polymer can suppress the bacterial adherence in oral cavity by a crossover design. We also investigated the number of Fusobacterium nucleatum, which is the key bacterium forming dental plaque, in clinical samples. This study was a randomized, placebo-controlled, single-blind, crossover study, with two treatment periods separated by a 2-week washout period. We conducted clinical trial with 20 healthy subjects to evaluate the effect of 5% MPC-polymer mouthwash after 5 h on oral microflora. PBS was used as a control. The bacterial number in the gargling sample before and after intervention was counted by an electronic bacterial counter and a culture method. DNA amounts of total bacteria and F. nucleatum were examined by q-PCR. The numbers of total bacteria and oral streptcocci after 5 h of 5% MPC-polymer treatment significantly decreased, compared to the control group. Moreover, the DNA amounts of total bacteria and F. nucleatum significantly decreased by 5% MPC-polymer mouthwash. We suggest that MPC-polymer coating in the oral cavity may suppress the oral bacterial adherence. MPC-polymer can be a potent compound for the control of oral microflora to prevent oral infection. (Keyword) 2-Methacryloyloxyethylphosphorylcholine / Clinical study / Oral infection / Oral bacteria / Fusobacterium nucleatum (Tokushima University Institutional Repository) ● Metadata: 113011 (Link to Publication Site) ● Publication site (DOI): 10.1007/s00784-018-2490-2 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29766378 ● Summary page in Scopus @ Elsevier: 2-s2.0-85046904798 (Tokushima University Institutional Repository: 113011, DOI: 10.1007/s00784-018-2490-2, PubMed: 29766378, Elsevier: Scopus)
5.	Takuya Nii, Hiromichi Yumoto, Katsuhiko Hirota and Yoichiro Miyake : Anti-inflammatory effects of olanexidine gluconate on oral epithelial cells, BMC Oral Health, Vol.19, No.Article number 239, 2019. (Tokushima University Institutional Repository) ● Metadata: 114367 (Link to Publication Site) ● Publication site (DOI): 10.1186/s12903-019-0932-0 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1186/s12903-019-0932-0 (Tokushima University Institutional Repository: 114367, DOI: 10.1186/s12903-019-0932-0)
6.	Hiromichi Yumoto, Katsuhiko Hirota, Kouji Hirao, Masami Ninomiya, Keiji Murakami, Hideki Fujii and Yoichiro Miyake : The pathogenic factors from oral Streptococci for systemic diseases, International Journal of Molecular Sciences, Vol.20, No.18, 2019. (Tokushima University Institutional Repository) ● Metadata: 114574 (Link to Publication Site) ● Publication site (DOI): 10.3390/ijms20184571 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85072542634 (Tokushima University Institutional Repository: 114574, DOI: 10.3390/ijms20184571, Elsevier: Scopus)
7.	Yoshihito Naitou, Hiromichi Yumoto, K Hs Kumar, Takashi Matsuo, Katsuhiko Hirota, Yoichiro Miyake, Kan Nagao, Yoritoki Tomotake, R Jimbo and Tetsuo Ichikawa : Antifungal and Mechanical Properties of Tissue Conditioner Containing Plant-Derived Component, --- An In Vitro Study ---, Journal of Prosthodontics, Vol.27, No.7, 665-669, 2018. (Summary) To evaluate the antifungal activity and mechanical properties of a novel antifungal tissue conditioner containing Juncus powder. Juncus powder was mixed with GC tissue conditioner at concentrations of 2.5%, 5.0%, and 10.0% by mass. The cylindrical specimens of Juncus-mixed tissue conditioner (dimensions: 10 mm in diameter and 2 and 6 mm in height for antimicrobial and mechanical tests, respectively) were prepared. The specimens placed on the bottom of the 24-well tissue culture plate were cultured with Candida albicans CAD1 for 2 and 4 days. The proliferation of the C. albicans in the wells was determined by measuring the optical density of fungal culture, and the surface of the specimens were also observed by scanning electron microscopy (SEM). To assess the mechanical properties of the specimens, the fluidity and hardness of Juncus-mixed tissue conditioner were measured using the methods certified according to ISO 10139-1. Juncus-mixed tissue conditioner significantly exhibited growth inhibitory effect in a Juncus concentration-dependent manner after both 2- and 4- day cultures. SEM observation showed that the amount of C. albicans on Juncus-mixed specimens drastically decreased, and biofilm formation was markedly inhibited. Moreover, both mechanical properties were found to be within the ranges regulated and specified by ISO. These findings demonstrated that the tissue conditioner including Juncus powder has a significant growth inhibitory effect against C. albicans, and it is suggested that the application of Juncus-mixed tissue conditioner may prevent denture stomatitis and oral candidiasis in denture wearers. (Link to Publication Site) ● Publication site (DOI): 10.1111/jopr.12546 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28901663 ● Search Scopus @ Elsevier (PMID): 28901663 ● Search Scopus @ Elsevier (DOI): 10.1111/jopr.12546 (DOI: 10.1111/jopr.12546, PubMed: 28901663)
8.	Susilowati Heni, Keiji Murakami, Hiromichi Yumoto, Takashi Amoh, Kouji Hirao, Katsuhiko Hirota, Takashi Matsuo and Yoichiro Miyake : Royal jelly inhibits Pseudomonas aeruginosa adherence and reduces excessive inflammatory responses in human epithelial cells, BioMed Research International, Vol.2017, 3191752, 2017. (Summary) is a Gram-negative bacterium and causes respiratory infection especially in elderly patients. Royal jelly has been used worldwide as a traditional remedy and as a nutrient; however, the effect against is unclear. The aim of this study was to analyze antibacterial, antiadherent, and anti-inflammatory effects of royal jelly against . Wild-type strain PAO1 and clinical isolates of were used for antibacterial assay and antiadherent assay to abiotic surface and epithelial cells, which are pharynx (Detroit 562) and lung (NCI-H292) epithelial cells. In anti-inflammatory assay, epithelial cells were pretreated with royal jelly before bacterial exposure to investigate its inhibitory effect on interleukin (IL-8) and macrophage inflammatory protein-3/CCL20 overproduction. Although royal jelly did not have antibacterial activity at concentration of 50% w/v, antiadherent activity was confirmed on the abiotic surface and epithelial cells under concentration of 25%. Pretreatment with royal jelly significantly inhibited overproduction of IL-8 and CCL20 from both cells. These results demonstrated that royal jelly inhibits adherence and protects epithelial cells from excessive inflammatory responses against infection. Our findings suggested that royal jelly may be a useful supplement as complementary and alternative medicine for preventing respiratory infection caused by . (Keyword) Cell Line / Chemokine CCL20 / Epithelial Cells / Fatty Acids / Gene Expression Regulation / Humans / inflammation / Interleukin-8 / Pseudomonas Infections / Pseudomonas aeruginosa (Tokushima University Institutional Repository) ● Metadata: 112385 (Link to Publication Site) ● Publication site (DOI): 10.1155/2017/3191752 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29075644 ● Summary page in Scopus @ Elsevier: 2-s2.0-85030761989 (Tokushima University Institutional Repository: 112385, DOI: 10.1155/2017/3191752, PubMed: 29075644, Elsevier: Scopus)
9.	Sekita Yasuko, Keiji Murakami, Hiromichi Yumoto, Kouji Hirao, Takashi Amoh, Natsumi Fujiwara, Katsuhiko Hirota, Hideki Fujii, Takashi Matsuo, Yoichiro Miyake and Yoshiki Kashiwada : Antibiofilm and Anti-Inflammatory Activities of+ Houttuynia cordata Decoction for Oral Care, Evidence-Based Complementary and Alternative Medicine : eCAM, Vol.2017, 2850947, 2017. (Summary) Dental biofilms that form in the oral cavity play a critical role in the pathogenesis of several infectious oral diseases, including dental caries, periodontal disease, and oral candidiasis. (HC, Saururaceae) is a widely used traditional medicine, for both internal and external application. A decoction of dried HC leaves (dHC) has long been consumed as a health-promoting herbal tea in Japan. We have recently reported that a water solution of HC poultice ethanol extract (wHCP) exerts antimicrobial and antibiofilm effects against several important oral pathogens. It also exhibits anti-inflammatory effects on human keratinocytes. In our current study, we examined the effects of dHC on infectious oral pathogens and inflammation. Our results demonstrated that dHC exerts moderate antimicrobial effects against methicillin-resistant (MRSA) and other oral microorganisms. dHC also exhibited antibiofilm effects against MRSA, (involved in dental plaque formation), and and inhibitory effects on interleukin-8, CCL20, IP-10, and GRO productions by human oral keratinocytes stimulated by lipopolysaccharide (a cause of periodontal disease), without cytotoxic effects. This suggests that dHC exhibits multiple activities in microorganisms and host cells. dHC can be easily prepared and may be effective in preventing infectious oral diseases. (Tokushima University Institutional Repository) ● Metadata: 112956 (Link to Publication Site) ● Publication site (DOI): 10.1155/2017/2850947 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29234378 ● Summary page in Scopus @ Elsevier: 2-s2.0-85042323475 (Tokushima University Institutional Repository: 112956, DOI: 10.1155/2017/2850947, PubMed: 29234378, Elsevier: Scopus)
10.	Takashi Amoh, Keiji Murakami, Kariyama Reiko, Hori Kenji, Vidoucic Darija, Katsuhiko Hirota, Igarashi Jun, Suga Hiroaki, Pasek R Matthew, Kumon Hiromi and Yoichiro Miyake : Effects of an autoinducer analogue on antibiotic tolerance in Pseudomonas aeruginosa, The Journal of Antimicrobial Chemotherapy, Vol.72, No.8, 2230-2240, 2017. (Summary) Antibiotic tolerance causes chronic, refractory and persistent infections. In order to advance the development of a new type of drug for the treatment of infectious diseases, we herein investigated the effects of a newly synthesized analogue of the Pseudomonas aeruginosa quorum-sensing autoinducer named AIA-1 ( a uto i nducer a nalogue) on antibiotic tolerance in P. aeruginosa . A P. aeruginosa luminescent strain derived from PAO1 was injected into neutropenic ICR mice and bioluminescence images were acquired for a period of time after treatments with antibiotics and AIA-1. In vitro susceptibility testing and killing assays for the planktonic and biofilm cells of PAO1 were performed using antibiotics and AIA-1. The expression of quorum-sensing-related genes was examined using real-time PCR. In vivo and in vitro assays showed that AIA-1 alone did not exert any bactericidal effects and also did not affect the MICs of antibiotics. However, the combined use of AIA-1 and antibiotics exerted markedly stronger therapeutic effects against experimental infection than antibiotics alone. The presence of AIA-1 also enhanced the killing effects of antibiotics in planktonic and biofilm cells. Although AIA-1 did not inhibit the expression of lasB and rhlA genes, which are directly regulated by quorum sensing, it clearly suppressed expression of the rpoS gene. The new compound, AIA-1, did not alter the antibiotic susceptibility of P. aeruginosa by itself; however, its addition enhanced the antibacterial activity of antibiotics. AIA-1 did not inhibit quorum sensing, but reduced the antibiotic tolerance of P. aeruginosa by suppressing rpoS gene expression. (Link to Publication Site) ● Publication site (DOI): 10.1093/jac/dkx132 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28510695 ● Search Scopus @ Elsevier (PMID): 28510695 ● Search Scopus @ Elsevier (DOI): 10.1093/jac/dkx132 (DOI: 10.1093/jac/dkx132, PubMed: 28510695)
11.	Natsumi Fujiwara, Keiji Murakami, Michiyasu Nakao, Munehisa Toguchi, Hiromichi Yumoto, Katsuhiko Hirota, Takashi Matsuo, Shigeki Sano, Kazumi Ozaki and Yoichiro Miyake : Antibacterial and antibiofilm effects of Reuterin-Related Compounds to periodontopathic bacteria, Journal of Oral Health and Biosciences, Vol.30, No.1, 8-17, 2017. (Tokushima University Institutional Repository) ● Metadata: 111063 (Link to Publication Site) ● Publication site (DOI): 10.20738/johb.30.1_8 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.20738/johb.30.1_8 (Tokushima University Institutional Repository: 111063, DOI: 10.20738/johb.30.1_8)
12.	Keiji Murakami, Tsuneko Ono, Darija Viducic, Yoko Somiya, Reiko Kariyama, Kenji Hori, Takashi Amoh, Katsuhiko Hirota, Hiromi Kumon, Matthew R. Parsek and Yoichiro Miyake : Role of psl Genes in Antibiotic Tolerance of Adherent Pseudomonas aeruginosa., Antimicrobial Agents and Chemotherapy, Vol.61, No.7, 2017. (Summary) Bacteria attached to a surface are generally more tolerant to antibiotics than their planktonic counterparts, even without the formation of a biofilm. The mechanism of antibiotic tolerance in biofilm communities is multifactorial, and the genetic background underlying this antibiotic tolerance has not yet been fully elucidated. Using transposon mutagenesis, we isolated a mutant with reduced tolerance to biapenem (relative to that of the wild type) from adherent cells. Sequencing analysis revealed a mutation in the pslL gene, which is part of the polysaccharide biosynthesis operon. The Pseudomonas aeruginosa PAO1 slBCD mutant demonstrated a 100-fold-lower survival rate during the exposure of planktonic and biofilm cells to biapenem; a similar phenotype was observed in a mouse infection model and in clinical strains. Transcriptional analysis of adherent cells revealed increased expression of both pslA and pelA, which are directly regulated by bis-(3',5')-cyclic dimeric GMP (c-di-GMP). Inactivation of wspF resulted in significantly increased tolerance to biapenem due to increased production of c-di-GMP. The loss of pslBCD in the spF mutant background abolished the biapenem-tolerant phenotype of the spF mutant, underscoring the importance of psl in biapenem tolerance. Overexpression of PA2133, which can catalyze the degradation of c-di-GMP, led to a significant reduction in biapenem tolerance in adherent cells, indicating that c-di-GMP is essential in mediating the tolerance effect. The effect of pslBCD on antibiotic tolerance was evident, with 50- and 200-fold-lower survival in the presence of ofloxacin and tobramycin, respectively. We speculate that the psl genes, which are activated by surface adherence through elevated intracellular c-di-GMP levels, confer tolerance to antimicrobials. (Link to Publication Site) ● Publication site (DOI): 10.1128/AAC.02587-16 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28438927 ● Search Scopus @ Elsevier (PMID): 28438927 ● Search Scopus @ Elsevier (DOI): 10.1128/AAC.02587-16 (DOI: 10.1128/AAC.02587-16, PubMed: 28438927)
13.	Kazuto Shiraishi, Noriko Saito-Tarashima, Yohsuke Igata, Keiji Murakami, Yasuko Okamoto, Yoichiro Miyake, Kazuhiro Furukawa and Noriaki Minakawa : Synthesis and evaluation of c-di-4'-thioAMP as an artificial ligand for c-di-AMP riboswitch, Bioorganic & Medicinal Chemistry, Vol.25, No.14, 3883-3889, 2017. (Summary) Cyclic-di-adenosine monophosphate (c-di-AMP) is a bacterial second messenger that binds to an RNA receptor called riboswitch and regulates its downstream genes involving cell wall metabolism, ion transport, and spore germination. Therefore, the c-di-AMP riboswitch can be a novel target of antibiotics. In this study, we synthesized c-di-4'-thioAMP (1), which possesses a sulfur atom instead of an oxygen atom in the furanose ring, as a candidate of a bioisoster for natural c-di-AMP. The resulting 1 bound to the c-di-AMP riboswitch with a micromolar affinity (34.8M), and the phosphodiesterase resistance of 1 was >12-times higher than that of c-di-AMP. Thus, 1 can be considered to be a stable ligand against a c-di-AMP riboswitch. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bmc.2017.05.042 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28559057 ● Search Scopus @ Elsevier (PMID): 28559057 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bmc.2017.05.042 (DOI: 10.1016/j.bmc.2017.05.042, PubMed: 28559057)
14.	Darija Viducic, Keiji Murakami, Takashi Amoh, Tsuneko Ono and Yoichiro Miyake : RpoN Promotes Pseudomonas aeruginosa Survival in the Presence of Tobramycin., Frontiers in Microbiology, Vol.8, 839, 2017. (Summary) Pseudomonas aeruginosa has developed diverse strategies to respond and adapt to antibiotic stress. Among the factors that modulate survival in the presence of antibiotics, alternative sigma factors play an important role. Here, we demonstrate that the alternative sigma factor RpoN ( (54)) promotes survival in the presence of tobramycin. The tobramycin-sensitive phenotype of logarithmic phase poN mutant cells is suppressed by the loss of the alternative sigma factor RpoS. Transcriptional analysis indicated that RpoN positively regulates the expression of RsmA, an RNA-binding protein, in the P. aeruginosa stationary growth phase in a nutrient-rich medium. The loss of RpoS led to the upregulation of gacA expression in the nutrient-limited medium-grown stationary phase cells. Conversely, in the logarithmic growth phase, the poS mutant demonstrated lower expression of gacA, underscoring a regulatory role of RpoS for GacA. Supplementation of tobramycin to stationary phase poN mutant cells grown in nutrient-rich medium resulted in decreased expression of gacA, relA, and rpoS without altering the expression of rsmA relative to wild-type PAO1. The observed downregulation of gacA and relA in the poN mutant in the presence of tobramycin could be reversed through the mutation of rpoS in the poN mutant background. The tobramycin-tolerant phenotype of the poN poS mutant logarithmic phase cells may be associated with the expression of relA, which remained unresponsive upon addition of tobramycin. The logarithmic phase poS and poN poS mutant cells demonstrated increased expression of gacA in response to tobramycin. Together, these results suggest that a complex regulatory interaction between RpoN, RpoS, the Gac/Rsm pathway, and RelA modulates the P. aeruginosa response to tobramycin. (Tokushima University Institutional Repository) ● Metadata: 114411 (Link to Publication Site) ● Publication site (DOI): 10.3389/fmicb.2017.00839 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28553272 ● Search Scopus @ Elsevier (PMID): 28553272 ● Search Scopus @ Elsevier (DOI): 10.3389/fmicb.2017.00839 (Tokushima University Institutional Repository: 114411, DOI: 10.3389/fmicb.2017.00839, PubMed: 28553272)
15.	Natsumi Fujiwara, Keiji Murakami, Michiyasu Nakao, Munehisa Toguchi, Hiromichi Yumoto, Takashi Amoh, Katsuhiko Hirota, Takashi Matsuo, Shigeki Sano, Kazumi Ozaki and Yoichiro Miyake : Novel reuterin-related compounds suppress odour by periodontopathic bacteria, Oral Diseases, Vol.23, No.4, 492-497, 2017. (Summary) Halitosis is caused by volatile sulphur compounds including methyl mercaptan (CH3 SH) in the oral cavity and is a serious problem that limits interpersonal social communication. The aim of study was to evaluate the effects of reuterin-related compounds (RRCs) on halitosis-related periodontopathic bacteria in vitro. RRC-01, RRC-02 and RRC-03 (32 and 64 g ml(-1) ) in culture media containing Fusobacterium nucleatum JCM8523 and Porphyromonas gingivalis ATCC33277 were used. The effects of RRCs on CH3 SH production and detectable odour by F. nucleatum and P. gingivalis were examined by CH3 SH production assay and organoleptic test, respectively. The number of bacterial cells was also measured using an ATP assay. In P. gingivalis treated with RRCs, the expression of mgl gene, which is responsible for CH3 SH production, was examined by qRT-PCR. CH3 SH production and the score of detectable odour from F. nucleatum and P. gingivalis culture media containing RRCs were significantly lower than that without RRCs (P < 0.05). The expression of mgl gene in P. gingivalis was significantly downregulated by RRC-01 (P < 0.01), but not by RRC-02 or RRC-03. RRCs are potent oral care products for preventing halitosis via reducing CH3 SH production. (Link to Publication Site) ● Publication site (DOI): 10.1111/odi.12638 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28083982 ● Summary page in Scopus @ Elsevier: 2-s2.0-85015084211 (DOI: 10.1111/odi.12638, PubMed: 28083982, Elsevier: Scopus)
16.	Keiji Murakami, Tsuneko Ono, Yasuki Noma, Issei Minase, Takashi Amoh, Yasuhiko Irie, Katsuhiko Hirota and Yoichiro Miyake : Explorative gene analysis of antibiotic tolerance-related genes in adherent and biofilm cells of Pseudomonas aeruginosa., Journal of Infection and Chemotherapy, Vol.23, No.5, 271-277, 2017. (Summary) Antibiotic tolerance has attracted worldwide attention, as it leads to chronic, refractory, and persistent infections that are difficult to control. Bacterial biofilms are well known to be more tolerant to antibiotics compared to planktonic bacteria. We previously revealed that adherent bacteria on a solid surface also exhibited tolerance to antibiotics before forming a biofilm. However, little is known about the mechanisms of antibiotic tolerance for adherent or biofilm cells. We investigated the mechanisms of antibiotic tolerance in the biofilm life cycle using adherent and biofilm cells, and evaluated the possibility that common mechanisms operate at each stage. We constructed transposon mutants of Pseudomonas aeruginosa PAO1 and screened for low-tolerant mutants with two different methods, using adherent cells and biofilm cells. Fourteen and nine mutants exhibiting low antibiotic tolerance were detected in the adherent cells and biofilm cells, and 14 and 7 candidate genes linked to this tolerance were identified by sequencing, respectively. Eight of the 14 genes related to the antibiotic tolerance of the adherent cells were involved in biofilm formation. Two of the seven genes related to the antibiotic tolerance of biofilm cells participated in the antibiotic tolerance of adherent cells. The antibiotic tolerance of adherent cells and biofilm formation appear to be under the same regulation mechanism to promote survival in the presence of antibiotics. Antibiotic tolerance shows a complex regulation mechanism at each stage of biofilm formation. (Keyword) Anti-Bacterial Agents / Biofilms / Humans / Microbial Sensitivity Tests / Mutation / Pseudomonas Infections / Pseudomonas aeruginosa (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jiac.2017.01.004 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28274550 ● Summary page in Scopus @ Elsevier: 2-s2.0-85014161002 (DOI: 10.1016/j.jiac.2017.01.004, PubMed: 28274550, Elsevier: Scopus)
17.	Darija Viducic, Keiji Murakami, Takashi Amoh, Tsuneko Ono and Yoichiro Miyake : Role of the interplay between quorum sensing regulator VqsR and the Pseudomonas quinolone signal in mediating carbapenem tolerance in Pseudomonas aeruginosa., Research in Microbiology, Vol.168, No.5, 450-460, 2017. (Summary) Pseudomonas aeruginosa coordinates its response to environmental conditions through activation of a quorum sensing (QS) system. In this study, we investigated the regulatory interaction between the QS transcriptional regulator VqsR and the Pseudomonas quinolone signal (PQS) through integration of sigma factor RpoS, and we addressed whether one of the pathways controlling carbapenem tolerance can be attributed to VqsR. We demonstrate that vqsR expression at the transcriptional level is regulated by pqsA, pqsR, and pqsE. Assessment of the transcriptional expression of vqsR, lasI, rhlI, and qscR in qsA and qsA poS mutants provided insight into pqsA- and rpoS-dependent regulation of vqsR and vqsR-controlled genes. Exogenously supplemented PQS reversed expression of vqsR and vqsR-controlled genes in the qsA mutant to wild-type levels, but failed to increase expression levels of lasI and qscR in the qsA poS mutant to levels observed in wild-type PAO1. The qsR mutant showed reduced survival when challenged with carbapenems compared to wild-type PAO1. Introduction of a pqsA mutation into the qsR mutant completely abolished its carbapenem-sensitive phenotype. We conclude that a regulatory link between PQS and vqsR exists, and that RpoS is important in their interaction. We also demonstrate that VqsR affects carbapenem tolerance. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.resmic.2017.02.007 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28263907 ● Search Scopus @ Elsevier (PMID): 28263907 ● Search Scopus @ Elsevier (DOI): 10.1016/j.resmic.2017.02.007 (DOI: 10.1016/j.resmic.2017.02.007, PubMed: 28263907)
18.	Keiji Murakami, Hiromichi Yumoto, Ayu Murakami, Takashi Amoh, Darija Viducic, Katsuhiko Hirota, Atsushi Tabata, Hideaki Nagamune, Hiroki Kourai, Takashi Matsuo and Yoichiro Miyake : Evaluation of the effectiveness of the potent bis-quaternary ammonium compound, 4,4'-( , -hexametylenedithio) bis (1-octylpyridinium bromide) (4DTBP-6,8) on Pseudomonas aeruginosa., Journal of Applied Microbiology, Vol.122, No.4, 893-899, 2017. (Summary) Quaternary ammonium compounds (QACs), including benzalkonium chloride (BAC) and cetylpyridinium chloride (CPC) are cationic surfactants and have been used widely as general disinfectants in the medical field due to their strong antibacterial effects and low cytotoxicity to human cells. 4,4'-( , -hexametylenedithio) bis (1-octylpyridinium bromide) (4DTBP-6,8) is one of the potent bis-QACs synthesized to improve the antimicrobial activities of mono-QACs such as BAC. This study aimed to assess the effectiveness of 4DTBP-6,8 against Pseudomonas aeruginosa, a prevalent hospital pathogen. The minimum inhibitory concentrations of 4DTBP-6,8, CPC and BAC against P. aeruginosa were measured. 4DTBP-6,8 exhibited strong antibacterial activity. We assessed the bactericidal effects of QACs against P. aeruginosa under certain conditions and their cytotoxicities in human epithelial cells using lactate dehydrogenase (LDH) release. 4DTBP-6,8 exerted excellent bactericidal effects against high concentrations of bacteria, biofilm cells and even in the presence of contaminated proteins. Cellular LDH was not released by the treatment with 4DTBP-6,8. 4DTBP-6,8 exhibited the strongest bactericidal activity against P. aeruginosa among the three QACs tested without any cytotoxicity. The potent bis-QAC, 4DTBP-6,8 has the potential to be an effective disinfectant in preventing hospital infections caused by P. aeruginosa. (Keyword) Anti-Bacterial Agents / Biofilms / Cell Line / Disinfectants / Humans / Niacinamide / Pseudomonas aeruginosa / Pyridinium Compounds (Link to Publication Site) ● Publication site (DOI): 10.1111/jam.13392 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28035713 ● Summary page in Scopus @ Elsevier: 2-s2.0-85013236835 (DOI: 10.1111/jam.13392, PubMed: 28035713, Elsevier: Scopus)
19.	Takashi Amoh, Keiji Murakami, Reiko Kariyama, Kenji Hori, Yasuhiko Irie, Darija Viducic, Katsuhiko Hirota, Jun Igarashi, Hiroaki Suga, Hiromi Kumon and Yoichiro Miyake : A Pseudomonas aeruginosa Quorum-Sensing autoinducer analog enhances the activity of antibiotics against resistant strains., The Journal of Medical Investigation : JMI, Vol.64, No.1.2, 101-109, 2017. (Summary) In this study, we have investigated the effects of the newly synthesized analog of Pseudomonas aeruginosa quorum-sensing autoinducer named AIA-1 (autoinducer analog) against antibiotic-resistant bacteria. In vitro susceptibility and killing assays for P. aeruginosa PAO1 prD mutant and clinical isolates were performed by using antibiotics and AIA-1. In an in vivo assay, a luminescent carbapenem-resistant strain derived from PAO1 prD was injected into neutropenic ICR mice and bioluminescence images were acquired after the treatment with antibiotics and AIA-1. Additionally, we investigated the effects of the combination use against carbapenem-resistant Enterobacteriaceae (CRE). Using killing assays in P. aeruginosa, the survival rates in the presence of antibiotics and AIA-1 significantly decreased in comparison with those with antibiotics alone. Furthermore, dual treatment of biapenem and AIA-1 was more effective than biapenem alone in a mouse infection model. AIA-1 did not change the MICs in P. aeruginosa, suggesting that AIA-1 acts on the mechanism of antibiotic tolerance. Conversely, the MICs of antibiotics decreased in the presence of AIA-1 in some CRE strains, indicating that AIA-1 may require additional mechanism to act on CRE. In conclusion, AIA-1 may be a potent drug for clinical treatment of infections caused by antibiotic-resistant bacteria. J. Med. Invest. 64: 101-109, February, 2017. (Tokushima University Institutional Repository) ● Metadata: 111094 (Link to Publication Site) ● Publication site (DOI): 10.2152/jmi.64.101 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28373605 ● Search Scopus @ Elsevier (PMID): 28373605 ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.64.101 (Tokushima University Institutional Repository: 111094, DOI: 10.2152/jmi.64.101, PubMed: 28373605)
20.	Katsuhiko Hirota, Hiromichi Yumoto, B Sapaar, Takashi Matsuo, Tetsuo Ichikawa and Yoichiro Miyake : Pathogenic factors in Candida biofilm-related infectious diseases., Journal of Applied Microbiology, Vol.122, No.2, 321-330, 2016. (Summary) Candida albicans is a commonly found member of the human microflora and is a major human opportunistic fungal pathogen. A perturbation of the microbiome can lead to infectious diseases caused by various micro-organisms, including C. albicans. Moreover, the interactions between C. albicans and bacteria are considered to play critical roles in human health. The major biological feature of C. albicans, which impacts human health, resides in its ability to form biofilms. In particular, the extracellular matrix (ECM) of Candida biofilm plays a multifaceted role and therefore may be considered as a highly attractive target to combat biofilm-related infectious diseases. In addition, extracellular DNA (eDNA) also plays a crucial role in Candida biofilm formation and its structural integrity and induces the morphological transition from yeast to the hyphal growth form during C. albicans biofilm development. This review focuses on pathogenic factors such as eDNA in Candida biofilm formation and its ECM production and provides meaningful information for future studies to develop a novel strategy to battle infectious diseases elicited by Candida-formed biofilm. (Keyword) Biofilms / Candida / Candida albicans / Candidiasis / DNA / Humans / Hyphae / quorum sensing / Respiratory Tract Infections / Virulence Factors (Link to Publication Site) ● Publication site (DOI): 10.1111/jam.13330 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27770500 ● Summary page in Scopus @ Elsevier: 2-s2.0-85005943779 (DOI: 10.1111/jam.13330, PubMed: 27770500, Elsevier: Scopus)
21.	Darija Viducic, Keiji Murakami, Takashi Amoh, Tsuneko Ono and Yoichiro Miyake : RpoN Modulates Carbapenem Tolerance in Pseudomonas aeruginosa through Pseudomonas Quinolone Signal and PqsE., Antimicrobial Agents and Chemotherapy, Vol.60, No.10, 5752-5764, 2016. (Summary) The ability of Pseudomonas aeruginosa to rapidly modulate its response to antibiotic stress and persist in the presence of antibiotics is closely associated with the process of cell-to-cell signaling. The alternative sigma factor RpoN ( (54)) is involved in the regulation of quorum sensing (QS) and plays an important role in the survival of stationary-phase cells in the presence of carbapenems. Here, we demonstrate that a poN mutant grown in nutrient-rich medium has increased expression of pqsA, pqsH, and pqsR throughout growth, resulting in the increased production of the Pseudomonas quinolone signal (PQS). The link between pqsA and its role in carbapenem tolerance was studied using a poN qsA mutant, in which the carbapenem-tolerant phenotype of the poN mutant was abolished. In addition, we demonstrate that another mechanism leading to carbapenem tolerance in the poN mutant is mediated through pqsE Exogenously supplied PQS abolished the biapenem-sensitive phenotype of the poN qsA mutant, and overexpression of pqsE failed to alter the susceptibility of the poN qsA mutant to biapenem. The mutations in the poN hlR mutant and the poN qsH mutant led to susceptibility to biapenem. Comparison of the changes in the expression of the genes involved in QS in wild-type PAO1 with their expression in the poN mutant and the poN mutant-derived strains demonstrated the regulatory effect of RpoN on the transcript levels of rhlR, vqsR, and rpoS The findings of this study demonstrate that RpoN negatively regulates the expression of PQS in nutrient-rich medium and provide evidence that RpoN interacts with pqsA, pqsE, pqsH, and rhlR in response to antibiotic stress. (Keyword) Bacterial Proteins / Carbapenems / Drug Resistance, Bacterial / Gene Expression Regulation, Bacterial / Microbial Sensitivity Tests / Mutation / Pseudomonas aeruginosa / Quinolones / quorum sensing / Sigma Factor / signal transduction / Trans-Activators (Link to Publication Site) ● Publication site (DOI): 10.1128/AAC.00260-16 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27431228 ● Summary page in Scopus @ Elsevier: 2-s2.0-84992643511 (DOI: 10.1128/AAC.00260-16, PubMed: 27431228, Elsevier: Scopus)
22.	Yasuko Sekita, Keiji Murakami, Hiromichi Yumoto, Takashi Amoh, Natsumi Fujiwara, Shohei Ogata, Takashi Matsuo, Yoichiro Miyake and Yoshiki Kashiwada : Preventive Effects of Houttuynia cordata Extract for Oral Infectious Diseases., BioMed Research International, Vol.2016, 2581876, 2016. (Summary) Houttuynia cordata (HC) (Saururaceae) has been used internally and externally as a traditional medicine and as an herbal tea for healthcare in Japan. Our recent survey showed that HC poultice (HCP) prepared from smothering fresh leaves of HC had been frequently used for the treatment of purulent skin diseases with high effectiveness. Our experimental study also demonstrated that ethanol extract of HCP (eHCP) has antibacterial, antibiofilm, and anti-inflammatory effects against S. aureus which caused purulent skin diseases. In this study, we focused on novel effects of HCP against oral infectious diseases, such as periodontal disease and dental caries. We determined the antimicrobial and antibiofilm effects of water solution of HCP ethanol extract (wHCP) against important oral pathogens and investigated its cytotoxicity and anti-inflammatory effects on human oral epithelial cells. wHCP had moderate antimicrobial effects against some oral microorganisms and profound antibiofilm effects against Fusobacterium nucleatum, Streptococcus mutans, and Candida albicans. In addition, wHCP had no cytotoxic effects and could inhibit interleukin-8 and CCL20 productions by Porphyromonas gingivalis lipopolysaccharide-stimulated human oral keratinocytes. Our findings suggested that wHCP may be clinically useful for preventing oral infectious diseases as a mouthwash for oral care. (Keyword) Anti-Bacterial Agents / bacteria / Biofilms / Cell Line / Cell Survival / Dental Caries / Houttuynia / Humans / Periodontal Diseases / Plant Extracts (Tokushima University Institutional Repository) ● Metadata: 112384 (Link to Publication Site) ● Publication site (DOI): 10.1155/2016/2581876 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27413739 ● Summary page in Scopus @ Elsevier: 2-s2.0-84978765486 (Tokushima University Institutional Repository: 112384, DOI: 10.1155/2016/2581876, PubMed: 27413739, Elsevier: Scopus)
23.	Yasuko Sekita, Keiji Murakami, Hiromichi Yumoto, Hiroyuki Mizuguchi, Takashi Amoh, Satoshi Ogino, Takashi Matsuo, Yoichiro Miyake, Hiroyuki Fukui and Yoshiki Kashiwada : Anti-bacterial and anti-inflammatory effects of ethanol extract from Houttuynia cordata poultice., Bioscience, Biotechnology, and Biochemistry, 2016. (Summary) Houttuynia cordata (HC) has been commonly used as many traditional remedies in local areas of Japan. Although many pharmacological activities of HC have been reported, the mechanism underlying the effect of HC remains unknown. We conducted the interview survey in Japan to verify how HC was actually used. The interview survey revealed that HC poultice (HCP) prepared from smothering fresh leaves of HC was most frequently used for the treatment of purulent skin diseases including furuncle and carbuncle with high effectiveness. Ethanol extract of HCP (eHCP) showed anti-bacterial effects against methicillin-resistant Staphylococcus aureus (MRSA), and showed an anti-biofilm activity against MRSA. eHCP showed dose-dependent inhibition of S. aureus lipoteichoic acid (LTA)-induced interleukin-8 and CCL20 production in human keratinocyte without any cytotoxicity. These results suggest that HCP is effective for skin abscess and its underlying mechanism might be the complicated multiple activities for both bacteria and host cells. (Link to Publication Site) ● Publication site (DOI): 10.1080/09168451.2016.1151339 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27023331 ● Search Scopus @ Elsevier (PMID): 27023331 ● Search Scopus @ Elsevier (DOI): 10.1080/09168451.2016.1151339 (DOI: 10.1080/09168451.2016.1151339, PubMed: 27023331)
24.	Haruyuki Nakayama-Imaohji, Katsuhiko Hirota, Hisashi Yamasaki, Saori Yoneda, Hirofumi Nariya, Motoo Suzuki, Thomas Secher, Yoichiro Miyake, Eric Oswald, Tetsuya Hayashi and Tomomi Kuwahara : DNA Inversion Regulates Outer Membrane Vesicle Production in Bacteroides fragilis., PLoS ONE, Vol.11, No.2, e0148887, 2016. (Summary) Phase changes in Bacteroides fragilis, a member of the human colonic microbiota, mediate variations in a vast array of cell surface molecules, such as capsular polysaccharides and outer membrane proteins through DNA inversion. The results of the present study show that outer membrane vesicle (OMV) formation in this anaerobe is also controlled by DNA inversions at two distantly localized promoters, IVp-I and IVp-II that are associated with extracellular polysaccharide biosynthesis and the expression of outer membrane proteins. These promoter inversions are mediated by a single tyrosine recombinase encoded by BF2766 (orthologous to tsr19 in strain NCTC9343) in B. fragilis YCH46, which is located near IVp-I. A series of BF2766 mutants were constructed in which the two promoters were locked in different configurations (IVp-I/IVp-II = ON/ON, OFF/OFF, ON/OFF or OFF/ON). ON/ON B. fragilis mutants exhibited hypervesiculating, whereas the other mutants formed only a trace amount of OMVs. The hypervesiculating ON/ON mutants showed higher resistance to treatment with bile, LL-37, and human β-defensin 2. Incubation of wild-type cells with 5% bile increased the population of cells with the ON/ON genotype. These results indicate that B. fragilis regulates the formation of OMVs through DNA inversions at two distantly related promoter regions in response to membrane stress, although the mechanism underlying the interplay between the two regions controlled by the invertible promoters remains unknown. (Keyword) Anti-Bacterial Agents / Bacterial Outer Membrane Proteins / Bacteroides fragilis / Cell Membrane / Drug Resistance, Bacterial / Extracellular Vesicles / Gene Expression Regulation, Bacterial / Promoter Regions, Genetic / proteomics / Sequence Inversion (Tokushima University Institutional Repository) ● Metadata: 112326 (Link to Publication Site) ● Publication site (DOI): 10.1371/journal.pone.0148887 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26859882 ● Summary page in Scopus @ Elsevier: 2-s2.0-84959440801 (Tokushima University Institutional Repository: 112326, DOI: 10.1371/journal.pone.0148887, PubMed: 26859882, Elsevier: Scopus)
25.	Omar Maningo Rodis, Edward Barroga, J Patrick Barron, James Hobbs, Jayanetti A. Jayawardena, Ikuo Kageyama, Bukasa Kalubi, Clive Langham, Yoshizo Matsuka, Yoichiro Miyake, Naoko Seki, Hiroko Oka, Martin Peters, Yo Shibata, Roxana Stegaroiu, Kazuyoshi Suzuki, Shigeru Takahashi, Hironori Tsuchiya, Toshiko Yoshida and Katsuhiko Yoshimoto : A proposed core curriculum for dental English education in Japan., BMC Medical Education, Vol.14, 239, 2014. (Summary) Globalization of the professions has become a necessity among schools and universities across the world. It has affected the medical and dental professions in terms of curriculum design and student and patient needs. In Japan, where medicine and dentistry are taught mainly in the Japanese language, profession-based courses in English, known as Medical English and Dental English, have been integrated into the existing curriculum among its 83 medical and 29 dental schools. Unfortunately, there is neither a core curriculum nor a model syllabus for these courses. This report is based on a survey, two discussion forums, a workshop, and finally, the drafting of a proposed core curriculum for dental English approved by consensus of the participants from each university. The core curriculum covers the theoretical aspects, including dental English terms and oral pathologies; and practical aspects, including blended learning and dentist-patient communication. It is divided into modules and is recommended to be offered for at least two semesters. The core curriculum is expected to guide curriculum developers in schools where dental English courses are yet to be offered or are still in their early development. It may also serve as a model curriculum to medical and dental schools in countries in Asia, Europe, Africa, and Central and South America, where English is not the medium of instruction. (Tokushima University Institutional Repository) ● Metadata: 114762 (Link to Publication Site) ● Publication site (DOI): 10.1186/s12909-014-0239-4 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25404383 ● Search Scopus @ Elsevier (PMID): 25404383 ● Search Scopus @ Elsevier (DOI): 10.1186/s12909-014-0239-4 (Tokushima University Institutional Repository: 114762, DOI: 10.1186/s12909-014-0239-4, PubMed: 25404383)
26.	Hiromichi Yumoto, Katsuhiko Hirota, Kouji Hirao, Tsuyoshi Miyazaki, Nobuyuki Yamamoto, Koji Miyamoto, Keiji Murakami, Natsumi Fujiwara, Takashi Matsuo and Yoichiro Miyake : Anti-inflammatory and protective effects of 2-methacryloyloxyethyl phosphorylcholine polymer on oral epithelial cells., Journal of Biomedical Materials Research. Part A, Vol.103, No.2, 555-563, 2014. (Summary) Periodontitis is a chronic inflammatory disease initiated by a microbial biofilm formed in the periodontal pocket. Gingival epithelium plays important roles as the first physical barrier to bacterial invasion and in orchestrating the innate immune reaction via toll-like receptors (TLRs), which recognize various bacterial products, and maintaining its function. Newly developed oral care products to inhibit bacterial adherence, subsequent inflammatory reaction and protect the gingival epithelium are expected. We previously reported that 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer coating decreased bacterial adhesion to human oral keratinocytes, RT-7, and mouth-rinsing with MPC-polymer inhibited the increase of oral bacteria. In this study, regarding the possibility of MPC-polymer application for preventing the adherence of periodontal pathogen, subsequent inflammatory reaction and protection of gingival epithelium, we examined the effects of MPC-polymer on the adherence of Porphyromonas gingivalis, major periodontitis-related pathogen, and TLR2 ligand to RT-7 and subsequent interleukin (IL)-8 production. MPC-polymer treatment significantly reduced P. gingivalis adherence by 44% and TLR2-mediated IL-8 production by blocking the binding of its specific-ligand in a concentration-dependent manner. Furthermore, MPC-polymer pretreatment protected RT-7 from injury by chemical irritants, cetylpyridinium chloride. These findings suggest that MPC-polymer is potentially useful for oral care to prevent oral infection and to maintain oral epithelial function. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2014. (Keyword) Anti-Inflammatory Agents / Bacteroidaceae Infections / Cell Line, Transformed / Epithelial Cells / Humans / Mouth / periodontitis / Phosphorylcholine / Polymethacrylic Acids / Porphyromonas gingivalis (Link to Publication Site) ● Publication site (DOI): 10.1002/jbm.a.35201 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24753309 ● Summary page in Scopus @ Elsevier: 2-s2.0-84924401882 (DOI: 10.1002/jbm.a.35201, PubMed: 24753309, Elsevier: Scopus)
27.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake : Effect of extracellular DNA on pyocyanin production from Pseudomonas aeruginosa, Bacterial Adherence & Biofilm, Vol.27, 45-47, 2014.
28.	狩山玲子, 堀賢司, 光畑律子, Keiji Murakami, 和田耕一郎, 石井亜矢乃, 渡辺豊彦, Yoichiro Miyake and 公文裕巳 : マウスを用いた緑膿菌性尿路バイオフィルム感染症モデルの作製, Bacterial Adherence & Biofilm, Vol.27, 67-71, 2014.
29.	B Sapaar, A Nur, Katsuhiko Hirota, Hiromichi Yumoto, Keiji Murakami, T Amoh, Takashi Matsuo, Tetsuo Ichikawa and Yoichiro Miyake : Effects of extracellular DNA from Candida albicans and pneumonia-related pathogens on Candida biofilm formation and hyphal transformation., Journal of Applied Microbiology, Vol.116, No.6, 1531-1542, 2014. (Summary) The aim of this study was to investigate the effects of genomic DNA purified from Candida albicans and pneumonia-related pathogens, Pseudomonas aeruginosa and Staphylococcus aureus, on in vitro biofilm formation and morphological change of 3 Candida species (C. albicans, C. glabrata, and C. tropicalis). Biofilm formation was evaluated by the crystal violet assay and colony-forming unit counts. Morphological characteristics of biofilms were evaluated by scanning electron microscopy and fluorescence microscopy. Addition of DNA at a low concentration (<1·0 μg ml(-1)) significantly increased biofilm mass of all three Candida species. In contrast, the addition of DNA at a high concentration (10 μg ml(-1)) decreased the biofilm mass. Interestingly, the formation of hyphae in a dense network of yeast cells was observed in C. albicans biofilms exposed to a low concentration of DNA (<1·0 μg ml(-1)). These findings demonstrated that extracellular DNA (eDNA) plays a crucial role in Candida biofilm formation and suggested that eDNA may induce the morphological transition from yeast to hyphal growth form during C. albicans biofilm development. A novel therapy targeting eDNA may be applicable for Candida infection to decrease biofilm formation and hyphal formation. (Link to Publication Site) ● Publication site (DOI): 10.1111/jam.12483 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24661775 ● Summary page in Scopus @ Elsevier: 2-s2.0-84900806168 (DOI: 10.1111/jam.12483, PubMed: 24661775, Elsevier: Scopus)
30.	Dali Liu, Yanbin Zhou, Mariko Naito, Hiromichi Yumoto, Qiang Li, Yoichiro Miyake, Jingping Liang and Rong Shu : Draft Genome Sequence of Porphyromonas gingivalis Strain SJD2, Isolated from Periodontal Pocket of a Patient with Periodontitis in China, Genome Announcements, Vol.2, No.1, e01091-13, 2014. (Summary) Porphyromonas gingivalis strain SJD2 was isolated from subgingival plaque of a patient in China with chronic periodontitis. Here, we report the draft genome of this strain, with a size of 2,328,850 bp, average G+C content of 48.3%, and 2,020 predicted protein-coding sequences. (Link to Publication Site) ● Publication site (DOI): 10.1128/genomeA.01091-13 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24385574 ● Summary page in Scopus @ Elsevier: 2-s2.0-84998610618 (DOI: 10.1128/genomeA.01091-13, PubMed: 24385574, Elsevier: Scopus)
31.	Katsuhiko Hirota, Satoru Inagaki, Ryo Hamada, Kazuyuki Ishihara and Yoichiro Miyake : Evaluation of a rapid oral bacteria quantification system using dielectrophoresis and the impedance measurement., Biocontrol Science, Vol.19, No.1, 45-49, 2014. (Summary) To clarify the availability of the dielectrophoretic impedance measurement (DEPIM) system as the evaluator for oral care, we evaluated the usefulness of DEPIM system by comparison with the standard plate counting (SPC) method. First, the relationship between the DEPIM results and bacterial concentration measured by SPC was clarified. Next, the measurement of the microorganism number in a mixed suspension was evaluated with DEPIM and SPC. The bacterial counts with DEPIM strongly correlated with those with SPC (r(2)=0.633-0.997) and this correlation was also shown in the measurement of a mixed bacterial suspension (ranging from 10(5) to 10(8) cfu/ml) of two bacterial species. Moreover, the experiments using dissociating enzymes to eliminate the influence of the size of the bacterial aggregates demonstrated that the microbial measurement results with DEPIM are unaffected by bacterial aggregates. This study demonstrated that bacterial counts with DEPIM strongly correlated with those with SPC and were unaffected by bacterial aggregates. (Link to Publication Site) ● Publication site (DOI): 10.4265/bio.19.45 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24670618 ● Summary page in Scopus @ Elsevier: 2-s2.0-84897509981 (DOI: 10.4265/bio.19.45, PubMed: 24670618, Elsevier: Scopus)
32.	Keiji Murakami, Tsuneko Ono, Katsuhiko Hirota and Yoichiro Miyake : 付着及びバイオフィルム形成緑膿菌の抗菌薬抵抗性関連遺伝子の探索, Bacterial Adherence & Biofilm, Vol.26, 237-238, 2013.
33.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake : Role of Extracellular DNA and DNA-binding protein in biofilm formation of Streptococcus intermeius, Bacterial Adherence & Biofilm, Vol.26, 25-29, 2013.
34.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, D Liu, Kanako Takahashi, Keiji Murakami, Takashi Matsuo, R Shu and Yoichiro Miyake : Effects of extracellular DNA and DNA-binding protein on the development of a Streptococcus intermedius biofilm., Journal of Applied Microbiology, Vol.115, No.1, 260-270, 2013. (Summary) The aim of this study was to clarify the effects of homologous and heterologous extracellular DNAs (eDNAs) and histone-like DNA-binding protein (HLP) on Streptococcus intermedius biofilm development and rigidity. Formed biofilm mass was measured with 0·1% crystal violet staining method and observed with a scanning electron microscope. The localizations of eDNA and extracellular HLP (eHLP) in formed biofilm were detected by staining with 7-hydoxyl-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) and anti-HLP antibody without fixation, respectively. DNase I treatment (200 U ml(-1) ) markedly decreased biofilm formation and cell density in biofilms. Colocalization of eHLP and eDNA in biofilm was confirmed. The addition of eDNA (up to 1 μg ml(-1) ) purified from Strep. intermedius, other Gram-positive bacteria, Gram-negative bacteria, or human KB cells into the Strep. intermedius culture increased the biofilm mass of all tested strains of Strep. intermedius, wild-type, HLP-downregulated strain and control strains. In contrast, the addition of eDNA (>1 μg ml(-1) ) decreased the biofilm mass of all Strep. intermedius strains. These findings demonstrated that eDNA and eHLP play crucial roles in biofilm development and its rigidity. eDNA- and HLP-targeting strategies may be applicable to novel treatments for bacterial biofilm-related infectious diseases. (Keyword) Biofilms / Cell Line, Tumor / DNA / DNA-Binding Proteins / Deoxyribonuclease I / Humans / Streptococcus intermedius (Tokushima University Institutional Repository) ● Metadata: 106279 (Link to Publication Site) ● Publication site (DOI): 10.1111/jam.12202 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23551549 ● Summary page in Scopus @ Elsevier: 2-s2.0-84879085342 (Tokushima University Institutional Repository: 106279, DOI: 10.1111/jam.12202, PubMed: 23551549, Elsevier: Scopus)
35.	Ikuko Haruta, Ken Kikuchi, Minoru Nakamura, Katsuhiko Hirota, Hidehito Kato, Hiroshi Miyakawa, Noriyuki Shibata, Yoichiro Miyake, Etsuko Hashimoto, Keiko Shiratori and Junji Yagi : Involvement of Commensal Bacteria may Lead to Dysregulated Inflammatory and Autoimmune Responses in a Mouse Model for Chronic Nonsuppurative Destructive Cholangitis., Journal of Clinical Immunology, Vol.32, No.5, 1026-1037, 2012. (Summary) BACKGROUND: We previously reported a mouse model of primary biliary cirrhosis (PBC)-like chronic nonsuppurative destructive cholangitis (CNSDC), in which frequent injections of Streptococcus intermedius induced CNSDC and autoantibody production. The present study was performed to verify the model by examining 1) the reappearance of the PBC-like CNSDC after lymphocyte transfer from model to naïve mice, 2) the involvement of autophagy, and 3) the influence of the strain difference. METHODS: Mice were inoculated with S. intermedius weekly for 8 weeks, then sacrificed to obtain samples. Spleen cells obtained from S. intermedius-inoculated mice were transferred to RAG2(-/-) mice. RESULTS: CNSDC and elevated serum level of anti-gp210 titers were observed in S. intermedius-inoculated C57BL/6 mice, similar to the results of our previous report using BALB/c mice. Portal inflammation was induced in the livers of RAG2(-/-) mice by the transfer of spleen cells from S. intermedius-inoculated C57BL/6 mice. Among the inflammatory cells in the RAG2(-/-) mice, CD3-positive cells were predominant. Autophagosome-like structures were detected histologically, in the cytoplasm of infiltrated cells around the bile ducts in the livers of S. intermedius-inoculated both C57BL/6 and BALB/c mice. In S. intermedius-inoculated C3H/HeJ mice, inflammation in the portal area was less extensive than that in the hepatic parenchyma. CONCLUSION: Bacterial component(s) and sequentially upregulated innate and acquired immune responses, accompanied by autophagy, might trigger CNSDC, via autoimmune mechanisms. Throughout the generation of bacteria-triggered PBC-like CNSDC, strain difference may influence the response to S. intermedius-inoculation in the liver. (Keyword) Adoptive Transfer / Animals / Antibodies, Antinuclear / DNA-Binding Proteins / Disease Models, Animal / female / Liver Cirrhosis, Biliary / Mice / Mice, Inbred BALB C / Mice, Inbred C3H / Mice, Inbred C57BL / knockout mice / Nuclear Pore Complex Proteins / Spleen / Streptococcal Infections / Streptococcus intermedius (Link to Publication Site) ● Publication site (DOI): 10.1007/s10875-012-9712-1 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22661269 ● Summary page in Scopus @ Elsevier: 2-s2.0-84866730182 (DOI: 10.1007/s10875-012-9712-1, PubMed: 22661269, Elsevier: Scopus)
36.	Jing Li, Katsuhiko Hirota, Takaharu Goto, Hiromichi Yumoto, Yoichiro Miyake and Tetsuo Ichikawa : Biofilm formation of Candida albicans on implant overdenture materials and its removal., Journal of Dentistry, Vol.40, No.8, 686-692, 2012. (Summary) The purposes of this study were to clarify the surface characteristics of various implant overdenture materials and the capabilities of Candida albicans adherence and biofilm formation on these surfaces, and to investigate the role of salivary mucin in biofilm formation. Seven commonly used implant and restorative materials were assessed. The surface roughness averages of all materials were limited to 0.07-0.10μm. Contact angles and salivary mucin absorption were measured. After 90-min initial adhesion and 2-day biofilm formation, the amounts of C. albicans were determined by counting colony-forming units and the morphological characteristics were observed by scanning electron microscopy (SEM). The effects of saliva coating and the influences of material surface property on initial adhesion, biofilm formation and its removability were analysed by univariate two-way analysis of variance and multiple linear regression analysis. Surface contact angle of materials, the index of hydrophobicity, was found to be correlated positively with initial adhesion and biofilm formation of C. albicans. A negative correlation between mucin absorption and removability of Candida biofilm indicates that mucin plays an important role in biofilm formation and its rigidity. SEM observation also revealed fewer Candida cells on saliva-coated Ti than on saliva-coated hydroxyapatite or acrylic resin. The materials with different hydrophobic property and compositions display diverse manners of salivary mucin absorption, initial adhesion and biofilm formation. The hydrophobic materials encourage enhanced initial adhesion, subsequently resulting in the active biofilm formation. Mucin has decisive effects on Candida immobilization and biofilm development on the materials. Surface hydrophilic property and composition of materials and salivary proteins, especially mucin, affect the process of Candida biofilm formation and influence the amount and rigidity of formed biofilm. The present data may be applied as a reference for selecting materials in implant overdenture treatment from a microbiological point of view. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jdent.2012.04.026 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22580351 ● Search Scopus @ Elsevier (PMID): 22580351 ● Search Scopus @ Elsevier (DOI): 10.1016/j.jdent.2012.04.026 (DOI: 10.1016/j.jdent.2012.04.026, PubMed: 22580351)
37.	Katsuhiko Hirota, Hiromichi Yumoto, K Miyamoto, N Yamamoto, Keiji Murakami, Yumi Hoshimo, Takashi Matsuo and Yoichiro Miyake : MPC-Polymer Reduces Adherence and Biofilm Formation by Oral Bacteria., Journal of Dental Research, Vol.90, No.7, 900-905, 2011. (Summary) Oral biofilms such as dental plaque cause dental caries and periodontitis, as well as aspiration pneumonia and infectious endocarditis by translocation. Hence, the suppression of oral biofilm formation is an issue of considerable importance. Mechanical removal, disinfectants, inhibition of polysaccharide formation, and artificial sugar have been used for the reduction of oral biofilm. From the viewpoint of the inhibition of bacterial adherence, we investigated whether aqueous biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer can reduce streptococcal colonization and biofilm formation. We examined the effects of MPC-polymer on streptococcal adherence to saliva-coated hydroxyapatite and oral epithelial cells, and the adherence of Fusobacterium nucleatum to streptococcal biofilm. MPC-polymer application markedly inhibited both the adherence and biofilm formation of Streptococcus mutans on saliva-coated hydroxyapatite and streptococcal adherence to oral epithelial cells, and reduced the adherence of F. nucleatum to streptococcal biofilms. A small-scale clinical trial revealed that mouthrinsing with MPC-polymer inhibited the increase of oral bacterial numbers, especially of S. mutans. These findings suggest that MPC-polymer is a potent inhibitor of bacterial adherence and biofilm development, and may be useful to prevent dental-plaque-related diseases. (UMIN Clinical Trial Registry UMIN000003471). Abbreviations: QOL, quality of life; MPC, 2-methacryloyloxyethyl phosphorylcholine; BHI, brain-heart infusion broth; PBS, phosphate-buffered saline; SEM, scanning electron microscopy; CFU, colony-forming unit. (Link to Publication Site) ● Publication site (DOI): 10.1177/0022034511402996 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21447697 ● Summary page in Scopus @ Elsevier: 2-s2.0-84884211413 (DOI: 10.1177/0022034511402996, PubMed: 21447697, Elsevier: Scopus)
38.	Heni Susilowati, Hirohiko Okamura, Katsuhiko Hirota, Masayuki Shono, Kaya Yoshida, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : Intermedilysin induces EGR-1 expression through calcineurin/NFAT pathway in human cholangiocellular carcinoma cells., Biochemical and Biophysical Research Communications, Vol.404, No.1, 57-61, 2011. (Summary) (Intermedilysin (ILY) is a cholesterol-dependent cytolysin produced by Streptococcus intermedius, which is associated with human brain and liver abscesses. Although intrahepatic bile duct cells play a valuable role in the pathogenesis of liver abscess, the molecular mechanism of ILY-treated intrahepatic bile duct cells remains unknown. In this study, we report that ILY induced a nuclear accumulation of intracellular calcium ([Ca(2+)]i) in human cholangiocellular cells HuCCT1. We also demonstrate that 10 ng/ml ILY induced NFAT1 dephosphorylation and its nuclear translocation in HuCCT1 cells. In contrast to the result that ILY induced NF-B translocation in human hepatic HepG2 cells, ILY did not affect NF-B localization in HuCCT1 cells. Dephosphorylation and nuclear translocation of NFAT1 caused by ILY were prevented by [Ca(2+)]i calcium chelator, BAPTA/AM, and calcineurin inhibitors, cyclosporine A and tacrolimus. ILY induced early growth response-1 (EGR-1) expression and it was inhibited by the pre-treatment with cyclosporine A, indicating that the calcineurin/NFAT pathway was involved in EGR-1 expression in response to ILY. ILY-induced calcineurin/NFAT1 activation and sequential EGR-1 expression might be related to the pathogenesis of S. intermedius in human bile duct cells.) (Keyword) Bacteriocins / Bile Ducts, Intrahepatic / Calcineurin / calcium / Cell Line, Tumor / Cell Nucleus / Early Growth Response Protein 1 / Humans / NFATC Transcription Factors (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbrc.2010.11.057 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21094139 ● Summary page in Scopus @ Elsevier: 2-s2.0-78650872240 (DOI: 10.1016/j.bbrc.2010.11.057, PubMed: 21094139, Elsevier: Scopus)
39.	J Li, Katsuhiko Hirota, Hiromichi Yumoto, Takashi Matsuo, Yoichiro Miyake and Tetsuo Ichikawa : Enhanced germicidal effects of pulsed UV-LED irradiation on biofilms., Journal of Applied Microbiology, Vol.109, No.6, 2183-2190, 2010. (Summary) This study can assist in developing a low-frequency pulsed UVA-LED system to be applied to pathogenic biofilms for disinfection. (Keyword) Biofilms / Candida albicans / Colony Count, Microbial / Disinfection / Escherichia coli / Free Radical Scavengers / Hydroxyl Radical / Mannitol / Microbial Viability / Ultraviolet Rays (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1365-2672.2010.04850.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20854456 ● Summary page in Scopus @ Elsevier: 2-s2.0-78449262889 (DOI: 10.1111/j.1365-2672.2010.04850.x, PubMed: 20854456, Elsevier: Scopus)
40.	Ikuko Haruta, Ken Kikuchi, Etsuko Hashimoto, Minoru Nakamura, Hiroshi Miyakawa, Katsuhiko Hirota, Noriyuki Shibata, Hidehito Kato, Yutaka Arimura, Yoichiro Kato, Takehiko Uchiyama, Hideaki Nagamune, Makio Kobayashi, Yoichiro Miyake, Keiko Shiratori and Junji Yagi : Long-term bacterial exposure can trigger nonsuppurative destructive cholangitis associated with multifocal epithelial inflammation., Laboratory Investigation; a Journal of Technical Methods and Pathology, Vol.90, No.4, 577-588, 2010. (Summary) Bacterial infection has become a focus of attention in the pathogenesis of primary biliary cirrhosis (PBC). We earlier reported that the bacterial lipoteichoic acid was detected at the sites of inflammation around damaged bile ducts in the livers of PBC, and PBC patients' sera showed high titers against streptococcal histone-like protein. Here, we investigated whether chronic bacterial exposure could trigger PBC-like epithelial cell damage in normal mouse. BALB/c mice were repeatedly inoculated with various bacteria for 8 weeks. At 1 week (Group 1) and 3, 4, or 20 months (long term; Group 2) after the final inoculation, mice were killed to obtain samples. In the livers of the Streptococcus intermedius (S.i.)-inoculated mice in Group 1, cellular infiltration was predominantly observed around the bile ducts over the hepatic parenchyma. In the S.i.-inoculated mice in Group 2, portal but not parenchymal inflammation was observed in the livers, and periductal cellular infiltrates were detected in the salivary glands. Both S.i.-inoculated Groups 1 and 2 BALB/c mice sera had antibodies against HuCCT1 biliary epithelial cells, anti-nuclear antibodies, and anti-gp210 antibodies, but not anti-mitochondrial antibodies. Immunoreactivity to histone-like DNA-binding protein of S.i. (S.i.-HLP) was detectable around the sites of chronic nonsuppurative destructive cholangitis in the portal area in the livers of both S.i.-inoculated Groups 1 and 2 BALB/c mice. Furthermore, anti-S.i.-HLP antibody bound to synthetic gp210 peptide, as well. Bacteria triggered PBC-like cholangitis, multifocal epithelial inflammation, and autoantibody production. Bacteria are likely involved in the pathogenesis of PBC and of associated multifocal epithelial inflammation. (Keyword) Animals / Antibodies, Antinuclear / Disease Models, Animal / Epithelial Cells / Immunity, Innate / Inflammation / Liver Cirrhosis, Biliary / Mice / Mice, Inbred BALB C / Nuclear Pore Complex Proteins / Streptococcus intermedius (Link to Publication Site) ● Publication site (DOI): 10.1038/labinvest.2010.40 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20142809 ● Search Scopus @ Elsevier (PMID): 20142809 ● Search Scopus @ Elsevier (DOI): 10.1038/labinvest.2010.40 (DOI: 10.1038/labinvest.2010.40, PubMed: 20142809)
41.	Yuka Yoshijima, Keiji Murakami, Shizuo Kayama, Dali Liu, Katsuhiko Hirota, Tetsuo Ichikawa and Yoichiro Miyake : Effect of substrate surface hydrophobicity on the adherence of yeast and hyphal Candida, Mycoses, Vol.53, No.3, 221-226, 2009. (Summary) A biofilm composed of various microorganisms including Candida is found on denture surfaces and is likely to be involved in the etiology of denture-induced stomatitis. The purpose of this study was to examine the role of hydrophobic interactions in candidal adherence to acrylic surfaces, particularly that of the hyphal form of Candida albicans. Candida clinical isolates were used. Acrylic plates coated with carrageenan and hydrocolloid (Hitachi chemical, Tokyo, Japan) were used as a hydrophilic substratum. A microbial suspension was placed on each acrylic plate and incubated. All plates were washed in phosphate-buffered saline containing CaCl(2) and MgCl(2) [PBS (+)] and cells still adhering to the acrylic surface were collected by 0.25% trypsin treatment. Cell-surface hydrophobicity was estimated using a modification of the technique used to measure adherence to hydrocarbons. When the acrylic plates were coated with hydrophilic materials, the adherence of hydrophobic clinical isolates of Candida and the hydrophobic hyphal C. albicans decreased, whereas the adherence of non-hydrophobic Candida was not affected or increased. We suggest that hydrophilic coating of denture surfaces could be a potent method for reduction of the adherence of relatively hydrophobic fungal cells, particularly hyphal C. albicans, which causes denture stomatitis and related infections. (Keyword) Candida albicans / Candidiasis / Carrageenan / Cell Adhesion / Colloids / Hydrophobic and Hydrophilic Interactions / Hyphae / Yeasts (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1439-0507.2009.01694.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19671080 ● Search Scopus @ Elsevier (PMID): 19671080 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1439-0507.2009.01694.x (DOI: 10.1111/j.1439-0507.2009.01694.x, PubMed: 19671080)
42.	Shizuo Kayama, Keiji Murakami, Tsuneko Ono, Makiko Ushimaru, Akitake Yamamoto, Katsuhiko Hirota and Yoichiro Miyake : The role of rpoS gene and quorum-sensing system in ofloxacin tolerance in Pseudomonas aeruginosa, FEMS Microbiology Letters, Vol.298, No.2, 184-192, 2009. (Summary) The basis of the bactericidal action of antibiotics and the mechanisms of antibiotic tolerance are largely unknown. To elucidate one of the mechanisms of antibiotic tolerance, the present study investigated the role of Pseudomonas aeruginosa quorum sensing (QS) and the rpoS gene in antibiotic tolerance. The survival rates of the lasR and lasI mutants were observed to be lower than that of the parental strain in time-dependent killing studies with 8 microg mL(-1) ofloxacin, but the survival rates of the rhlR and rhlI mutants were not different from that of the parental strain. Moreover, a lasR-overexpressing strain was more tolerant to ofloxacin than the parental strain, but this was not the case for an rhlR-overexpressing strain. The mRNA expression levels of lasR, lasI, and rpoS in the wild-type strain in the presence of bactericidal concentration of ofloxacin were lower than that in the absence of ofloxacin. In addition, the significant loss of antibiotic tolerance in the lasR mutant was recovered by the overexpression of rpoS. These results suggest that the Las QS system in P. aeruginosa is involved in the development of ofloxacin tolerance, and the tolerance induced by the Las-system is regulated by rpoS gene. (Keyword) Anti-Bacterial Agents / Bacterial Proteins / Gene Deletion / Gene Expression Profiling / Ligases / Microbial Viability / Ofloxacin / Pseudomonas aeruginosa / quorum sensing / Sigma Factor / Time Factors / Trans-Activators / Transcription Factors (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1574-6968.2009.01717.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19645822 ● Summary page in Scopus @ Elsevier: 2-s2.0-68949207968 (DOI: 10.1111/j.1574-6968.2009.01717.x, PubMed: 19645822, Elsevier: Scopus)
43.	Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Shizuo Kayama, Taniguchi Tomonori, Yamamoto Akitake, Tsuneko Ono, Takashi Matsuo and Yoichiro Miyake : Heterologous expression of a histone-like protein from Streptococcus intermedius in Escherichia coli alters the nucleoid structure and inhibits the growth of E. coli, FEMS Microbiology Letters, Vol.288, No.1, 68-75, 2008. (Summary) Escherichia coli failed to survive after transformation with a Streptococcus intermedius histone-like protein gene (Si-hlp) and its promoter-harbored plasmid. The promoter function of Si-hlp in E. coli was determined using enhanced green fluorescence protein (egfp) gene as a reporter. The inhibitory effect of Si-HLP on E. coli viability was verified by a tetracycline-inducible gene expression system. Further study suggested that Si-HLP may alter the bacterial nucleoid structure, leading to the growth inhibition of E. coli. (Keyword) Bacterial Proteins / Cell Nucleolus / Cloning, Molecular / DNA, Bacterial / DNA, Superhelical / Escherichia coli / gene expression / Histones / Streptococcus intermedius (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1574-6968.2008.01327.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18761672 ● Summary page in Scopus @ Elsevier: 2-s2.0-52649125775 (DOI: 10.1111/j.1574-6968.2008.01327.x, PubMed: 18761672, Elsevier: Scopus)
44.	A Ishikawa, T Yoneyama, Katsuhiko Hirota, Yoichiro Miyake and K Miyatake : Professional oral health care reduces the number of oropharyngeal bacteria, Journal of Dental Research, Vol.87, No.6, 594-598, 2008. (Summary) Silent aspiration of oropharyngeal pathogenic organisms is a significant risk factor causing pneumonia in the elderly. We hypothesized that regular oral hygiene care will affect the presence of oropharyngeal bacteria. Professional cleaning of the oral cavity and/or the gargling of a disinfectant liquid solution was performed over a five-month period in three facilities for the dependent elderly. Total oropharyngeal bacteria, streptococci, staphylococci, Candida, Pseudomonas, and black-pigmented Bacteroides species were monitored. The levels of these oropharyngeal bacteria decreased or disappeared after weekly professional oral health care, i.e., cleaning of teeth, dentures, tongue, and oral mucous membrane by dental hygienists. After lunch, gargling with povidone iodine was shown to be less effective than professional oral care. These findings indicate that weekly professional mechanical cleaning of the oral cavity, rather than a daily chemical disinfection of the mouth, can be an important strategy to prevent aspiration pneumonia in the dependent elderly. (Keyword) Aged / Aged, 80 and over / Colony Count, Microbial / Dental Care for Aged / Dental Prophylaxis / female / Humans / male / Nursing Homes / Oropharynx / Pneumonia, Aspiration / Pneumonia, Bacterial (Link to Publication Site) ● Publication site (DOI): 10.1177/154405910808700602 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18502972 ● Search Scopus @ Elsevier (PMID): 18502972 ● Search Scopus @ Elsevier (DOI): 10.1177/154405910808700602 (DOI: 10.1177/154405910808700602, PubMed: 18502972)
45.	Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Tsuneko Ono, Hideaki Nagamune, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake : The essentiality and involvement of Streptococcus intermedius histone-like DNA-binding protein in bacterial viability and normal growth, Molecular Microbiology, Vol.68, No.5, 1268-1282, 2008. (Summary) Streptococcus intermedius histone-like DNA-binding protein (Si-HLP) is a homodimeric protein and, conserved with Escherichia coli HU, a well-documented nucleoid-associated protein (NAP). In E. coli, HU plays important roles as both structural and regulatory factors, but it is not essential for E. coli viability. Streptococcal HLP has been found to bind host cells and induce cytokine production, but its physiological role remains poorly defined. In the present study, using gene insertion knockout and tetracycline-regulated antisense RNA expression techniques, we determined whether Si-HLP is essential for bacterial viability and normal growth in S. intermedius. The Si-HLP-downregulated S. intermedius strain showed alterations in its morphology and surface properties. Downregulation of Si-HLP led to an expanded nucleoid to fill the intracellular space. Transcription levels of several genes, including virulence-associated factors, were found to be activated or repressed in the antisense Si-hlp RNA-expressing strain by real-time PCR and reverse-transcription PCR. Collectively, these data suggest that Si-HLP serves as an essential NAP governing the nucleoid architecture and controlling the gene transcription profile in S. intermedius. (Keyword) Bacterial Proteins / DNA-Binding Proteins / Escherichia coli Proteins / Gene Expression Regulation, Bacterial / Histones / Microbial Viability / Streptococcus intermedius / Transcription, Genetic (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1365-2958.2008.06232.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18410499 ● Summary page in Scopus @ Elsevier: 2-s2.0-43749117518 (DOI: 10.1111/j.1365-2958.2008.06232.x, PubMed: 18410499, Elsevier: Scopus)
46.	Haruta Ikuko, Kikuchi Ken, Hashimoto Etsuko, Kato Hidehito, Katsuhiko Hirota, Kobayashi Makio, Yoichiro Miyake, Uchiyama Takehiko, Yagi Junji and Shiratori Keiko : A Possible role of histone-like DNA-binding protein of Streptococcus intermedius in the pathogenesis of bile duct damage in primary biliary cirrhosis, Clinical Immunology, Vol.127, No.2, 245-251, 2008. (Summary) Bacterial infection has become a focus of attention in the pathogenesis of primary biliary cirrhosis (PBC). It was reported that anti-histone autoantibody was detected in PBC, suggesting that bacterial histone-like DNA-binding protein (HLP) may be involved in the pathogenesis of PBC. To identify bacterial species in PBC to confirm this possibility, serum reactivity to bacterial cells was studied by ELISA. The IgM class Streptococcus intermedius titers were significantly higher in PBC than chronic hepatitis due to hepatitis C virus (CH-C) and healthy subjects. Among the streptococci, S. intermedius was selected for further study. The antigenic peptide of S. intermedius of HLP was synthesized to examine the serum reactivity to Si-HLP. IgM class anti-Si-HLP peptide titers were significantly higher in PBC. Immunoreactivity to anti-Si-HLP was detected in the cytoplasm of biliary epithelial cells and inflammatory cells in the portal area in PBC patients' livers. Streptococci, especially S. intermedius, might play a key role in the pathogenesis of PBC, possibly involving HLP. (Keyword) Adult / Aged / Antibodies, Bacterial / Biopsy / DNA-Binding Proteins / Enzyme-Linked Immunosorbent Assay / female / Humans / Immunohistochemistry / Liver Cirrhosis, Biliary / male / Middle Aged / Streptococcal Infections / Streptococcus intermedius (Link to Publication Site) ● Publication site (DOI): 10.1016/j.clim.2008.01.010 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18337173 ● Summary page in Scopus @ Elsevier: 2-s2.0-41549133879 (DOI: 10.1016/j.clim.2008.01.010, PubMed: 18337173, Elsevier: Scopus)
47.	Dali Liu, Hiromichi Yumoto, Katsuhiko Hirota, Keiji Murakami, Kanako Takahashi, Kouji Hirao, Takashi Matsuo, Kazuto Ohkura, Hideaki Nagamune and Yoichiro Miyake : Histone-like DNA binding protein of Streptococcus intermedius induces the expression of pro-inflammatory cytokines in human monocytes via action of ERK1/2 and JNK pathways, Cellular Microbiology, Vol.10, No.1, 262-276, 2008. (Summary) Streptococcus intermedius is a commensal associated with serious, deep-seated purulent infections in major organs, such as the brain and liver. Histone-like DNA binding protein (HLP) is an accessory architectural protein in a variety of bacterial cellular processes. In this study, we investigated the mechanisms of pro-inflammatory cytokine inductions in THP-1 cells by stimulation with recombinant HLP of S. intermedius (rSi-HLP). rSi-HLP stimulation-induced production of pro-inflammatory cytokines (IL-8, IL-1 beta and TNF-alpha) occurred in a time- and dose-dependent manner. In contrast with the heat-stable activity of DNA binding, the induction activity of rSi-HLP was heat-unstable. In subsequent studies, rSi-HLP acted cooperatively with lipoteichoic acid, the synthetic Toll-like receptor 2 agonist, Pam3CSK4, and the cytosolic nucleotide binding oligomerization domain 2 receptor agonist, muramyldipeptide. Furthermore, Western blot and blocking assays with specific inhibitors showed that rSi-HLP stimulation induced the activation of cell signal transduction pathways, extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinase (JNK). In addition to its physiological role in bacterial growth through DNA binding, these results indicate that Si-HLP can trigger a cascade of events that induce pro-inflammatory responses via ERK1/2 and JNK signal pathways, and suggest that bacterial HLP may contribute to the activation of host innate immunity during bacterial infection. (Keyword) Acetylmuramyl-Alanyl-Isoglutamine / Bacterial Proteins / Cell Line / Cytokines / DNA, Bacterial / DNA-Binding Proteins / Hot Temperature / Humans / JNK Mitogen-Activated Protein Kinases / Lipopolysaccharides / Mitogen-Activated Protein Kinase 3 / Molecular Sequence Data / Monocytes / Recombinant Proteins / Sequence Analysis, DNA / Signal Transduction / Streptococcus intermedius / Teichoic Acids / Up-Regulation (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1462-5822.2007.01040.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17883418 ● Search Scopus @ Elsevier (PMID): 17883418 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1462-5822.2007.01040.x (DOI: 10.1111/j.1462-5822.2007.01040.x, PubMed: 17883418)
48.	Darija Viducic, Tsuneko Ono, Keiji Murakami, Katakami Mikiko, Susilowati Heni and Yoichiro Miyake : rpoN gene of Pseudomonas aeruginosa alters Its susceptibility to quinolones and cabapenems, Antimicrobial Agents and Chemotherapy, Vol.51, No.4, 1455-1462, 2007. (Summary) The alternative sigma factor sigma54 has been implicated in diverse functions within the cells. In this study, we have constructed an rpoN mutant of Pseudomonas aeruginosa and investigated its importance as a target for antimicrobial agents, such as quinolones and carbapenems. The stationary-phase cells of the rpoN mutant displayed a survival rate approximately 15 times higher than that of the wild-type cells in the presence of quinolones and carbapenems. The stationary phase led to substantial production of pyoverdine by the P. aeruginosa rpoN mutant. Pyoverdine synthesis correlated with decreased susceptibility to antimicrobial agents. Quantitative real-time PCR revealed that stationary-phase cells of the rpoN mutant grown without an antimicrobial agent had approximately 4- to 140- and 2- to 14-fold-higher levels of transcripts of the pvdS and vqsR genes, respectively, than the wild-type strain. In the presence of an antimicrobial agent, levels of pvdS and vqsR transcripts were elevated 400- and 5-fold, respectively, in comparison to the wild-type levels. Flow cytometry assays using a green fluorescent protein reporter demonstrated increased expression of the vqsR gene in the rpoN mutant throughout growth. A pvdS mutant of P. aeruginosa, deficient in pyoverdine production, was shown to be susceptible to biapenem. These findings suggest that rpoN is involved in tolerance to antimicrobial agents in P. aeruginosa and that its tolerant effect is partly dependent on increased pyoverdine production and vqsR gene expression. (Keyword) Carbapenems / Gene Expression Regulation, Bacterial / Genes, Bacterial / Promoter Regions, Genetic / Pseudomonas aeruginosa / Quinolones / RNA Polymerase Sigma 54 / Transcription, Genetic / Virulence (Link to Publication Site) ● Publication site (DOI): 10.1128/AAC.00348-06 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17261620 ● Summary page in Scopus @ Elsevier: 2-s2.0-34247154268 (DOI: 10.1128/AAC.00348-06, PubMed: 17261620, Elsevier: Scopus)
49.	Darija Viducic, Tsuneko Ono, Keiji Murakami, Susilowati Heni, Shizuo Kayama, Katsuhiko Hirota and Yoichiro Miyake : Functional Analysis of spoT, relA and dksA Genes on Quinolone Tolerance in Pseudomonas aeruginosa under Nongrowing Condition, Microbiology and Immunology, Vol.50, No.4, 349-357, 2006. (Summary) To assess the contribution of ppGpp in antibiotic tolerance to quinolone in Pseudomonas aeruginosa, knockout mutants of the genes involved or linked with the stringent response, such as relA, spoT and dksA, were constructed and investigated for their antibiotic susceptibility to quinolones. The survival of the dksA and spoT mutants in the presence of 8 microg/ml of ofloxacin and 1 microg/ml of ciprofloxacin were shown to be approximately 20-180 and 10-40 times respectively, higher than the same for the wild type strain. The intracellular levels of ppGpp determined with high performance liquid chromatography (HPLC) demonstrated that spoT and dksA mutants possess higher basal levels of ppGpp. The data suggest that elevated basal levels of ppGpp may be responsible for rendering these mutants tolerant to quinolones and expand the importance of ppGpp as an antimicrobial target in P. aeruginosa. (Keyword) Ciprofloxacin / Drug Resistance / Guanosine Tetraphosphate / Microbial Sensitivity Tests / Ofloxacin / Pseudomonas aeruginosa / Quinolones (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16625057 ● Search Scopus @ Elsevier (PMID): 16625057 (PubMed: 16625057)
50.	Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae, Keiji Murakami, Yoichiro Miyake and Takashi Matsuo : CXCL12 and CXCR4 expression by human gingival fibroblasts in periodontal disease, Clinical and Experimental Immunology, Vol.141, No.3, 467-474, 2005. (Summary) CXCL12 is a CXC chemokine that is related to lymphocyte infiltration and angiogenesis in inflammatory sites such as arthritis. However, the expression and roles of CXCL12 in periodontal disease are uncertain. The aim of this study was to assess the expression of CXCL12 and its receptor, CXCR4, in periodontal tissue and to investigate the properties of CXCL12 and CXCR4 expression by human gingival fibroblasts (HGF). RT-PCR analysis revealed that CXCL12 and CXCR4 mRNA were expressed in both normal gingival tissues and periodontal diseased tissues. Immunohistochemistry disclosed that CXCL12 was expressed and CXCR4 positive cells were found in both normal and periodontal diseased gingival tissues. Our in vitro experiments elucidated that HGF constitutively produced CXCL12, and the levels were enhanced by stimulation with tumour necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), transforming growth factor-beta (TGF-beta), regulated upon activation normal T cell expressed and secreted (RANTES) and macrophage inflammatory protein 3(alpha) (MIP-3(alpha)). On the other hand, heat killed Porphyromonas gingivalis (P. gingivalis) and P. gingivalis LPS reduced the CXCL12 production by HGF. Flow cytometry analysis clarified that CXCR4 was highly expressed on HGF, and CXCR4 expression was abrogated by TNF-alpha, IFN-gamma and P. gingivalis LPS. Moreover, CXCL12 induced vascular endothelial growth factor (VEGF) production by HGF. Our results demonstrated that CXCL12 might be related to CXCR4+ cells infiltration and angiogenesis both in normal periodontal tissues and periodontal diseased tissue. P. gingivalis, a known periodontal pathogen, inhibits the production of CXCL12 and the expression of CXCR4 by HGF. This fact means that P. gingivalis may inhibit CXCR4+ cells infiltration and neovascularization in periodontal tissue and escape from the immune response. (Keyword) CXCL12 / CXCR4 / fibroblast / periodontal disease / Porphyromonas gingivalis (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1365-2249.2005.02852.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16045736 ● Search Scopus @ Elsevier (PMID): 16045736 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2249.2005.02852.x (DOI: 10.1111/j.1365-2249.2005.02852.x, PubMed: 16045736)
51.	Katsuhiko Hirota, Keiji Murakami, Ken Nemoto and Yoichiro Miyake : Coating of a surface with 2-methacryloyloxyethyl phosphorylcholine (MPC) co-polymer significantly reduces retention of human pathogenic microorganisms, FEMS Microbiology Letters, Vol.248, No.1, 37-45, 2005. (Summary) The present study compares the retention of four species that are often isolated in association with biomedical device-related infections - Staphylococcus aureus, Streptococcus mutans, Pseudomonas aeruginosa, and Candida albicans - to three different surfaces. All four bacterial species were found to bind significantly less well to MPC-coated surfaces than to non-coated surfaces. We attribute this effect to the "superhydrophilicity" of MPC-coated surfaces, whereas hydrophobic surfaces are well known to reduce bacterial retention and thus to inhibit a crucial step in the formation of bacterial biofilms that lead to biomedical device-related infections and complications. (Keyword) 2-Methacryloyloxyethyl phosphorylcholine / Superhydrophilicity / Hydrophobic / Microbial retention (Link to Publication Site) ● Publication site (DOI): 10.1016/j.femsle.2005.05.019 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15953697 ● Summary page in Scopus @ Elsevier: 2-s2.0-20444482366 (DOI: 10.1016/j.femsle.2005.05.019, PubMed: 15953697, Elsevier: Scopus)
52.	Akiko Sukeno, Hideaki Nagamune, Robert A. Whiley, Syed I. Jafar, Joseph Aduse-Opoku, Kazuto Ohkura, Takuya Maeda, Katsuhiko Hirota, Yoichiro Miyake and Hiroki Kourai : Intermedilysin Is Essential for the Invasion of Hepatoma HepG2 Cells by Streptococcus intermedius, Microbiology and Immunology, Vol.49, No.7, 681-694, 2005. (Summary) Streptococcus intermedius causes endogenous infections leading to abscesses. This species produces intermedilysin (ILY), a human-specific cytolysin. Because of the significant correlation between higher ILY production levels by S. intermedius and deep-seated abscesses, we constructed ily knockout mutant UNS38 B3 and complementation strain UNS38 B3R1 in order to investigate the role of ILY in deep-seated infections. Strain UNS38 reduced the viability of human liver cell line HepG2 at infection but not of rat liver cell line BRL3A. Isogenic mutant strain UNS38 B3 was not cytotoxic in either cell line. Quantification of S. intermedius revealed that in infected HepG2 cells UNS38 but not UNS38 B3 increased intracellularly concomitantly with increasing cell damage. This difference between UNS38 and UNS38 B3 was not observed with UNS38 B3R1. Invasion and proliferation in BRL3A cells was not observed. Masking UNS38 or UNS38 B3R1 with ILY antibody drastically decreased adherence and invasion of HepG2. Moreover, coating strain UNS38 B3 with ILY partially restored adherence to HepG2 but without subsequent bacterial growth. At 1 day post-infection, many intact UNS38 were detected in the damaged phagosomes of HepG2 with bacterial proliferation observed in the cytoplasm of dead HepG2 after an additional 2 day incubation. These results indicate that surface-bound ILY on S. intermedius is an important factor for invasion of human cells by this bacterium and that secretion of ILY within host cells is essential for subsequent host cell death. These data strongly implicate ILY as an important factor in the pathogenesis of abscesses in vivo by this streptococcus. (Keyword) Animals / Bacterial Proteins / Bacterial Toxins / Bacteriocins / Carcinoma, Hepatocellular / Cytotoxins / Humans / Streptococcal Infections / Streptococcus intermedius / Tumor Cells, Cultured (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16034212 ● Search Scopus @ Elsevier (PMID): 16034212 (PubMed: 16034212)
53.	Keiji Murakami, Tsuneko Ono, Darija Viducic, Shizuo Kayama, Makiko Mori, Katsuhiko Hirota, Ken Nemoto and Yoichiro Miyake : Role for rpoS gene of Pseudomonas aeruginosa in antibiotic tolerance, FEMS Microbiology Letters, Vol.242, No.1, 161-167, 2005. (Summary) The alternative sigma factor, RpoS has been described as a central regulator of many stationary phase-inducible genes and a master stress-response regulator under various stress conditions. We constructed an rpoS mutant in Pseudomonas aeruginosa and investigated the role of rpoS gene in antibiotic tolerance. The survival of the rpoS mutant cells in stationary phase was approximately 70 times lower when compared with that of the parental strain at 37 degrees C for 2 h after the addition of biapenem. For imipenem, the survival was approximately 40 times lower. Heat stress promoted an increase in the survival of the parental strain to biapenem, but the same was not found to be the case for the rpoS mutant. Our results indicate that rpoS gene is involved in tolerance to antibiotics in P. aeruginosa during the stationary phase and heat stress. However, under osmotic stress, tolerance to biapenem was not dependent on the rpoS gene. (Keyword) Pseudomonas aeruginosa / Tolerance / rpoS gene (Link to Publication Site) ● Publication site (DOI): 10.1016/j.femsle.2004.11.005 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15621433 ● Search Scopus @ Elsevier (PMID): 15621433 ● Search Scopus @ Elsevier (DOI): 10.1016/j.femsle.2004.11.005 (DOI: 10.1016/j.femsle.2004.11.005, PubMed: 15621433)
54.	Hideaki Nagamune, Kazuto Ohkura, Akiko Sukeno, Graeme Cowan, Timothy J. Mitchell, Wataru Ito, Ooki Ohnishi, Kanako Hattori, Miki Yamato, Katsuhiko Hirota, Yoichiro Miyake, Takuya Maeda and Hiroki Kourai : The human-specific action of intermedilysin, a homolog of streptolysin O, is dictated by domain 4 of the protein., Microbiology and Immunology, Vol.48, No.9, 677-692, 2004. (Summary) ヒト特異的細胞溶解毒素インターメディリシン(ILY)はストレプトリシンO(SLO)と同様にコレステロール(CHL)結合性細胞溶解毒素遺伝子ファミリーに属するが，CHLとの直接結合能を持たず，そのドメイン4でヒト細胞と結合することを明らかとした．またILYとSLO等の同属毒素間で多数のキメラ毒素を作成してその活性を調べた結果，SLO等ではCHLを結合する11アミノ酸領域は直接ヒト細胞の認識には関わらず，ここを除くドメイン4のC末端側56アミノ酸残基中にヒト細胞の受容体を認識する部位があることを示した． (Keyword) Amino Acid Sequence / Animals / Bacterial Proteins / Bacteriocins / Binding Sites / Cholesterol / Erythrocyte Membrane / Hemolysis / Humans / Liposomes / Microscopy, Electron, Transmission / Models, Molecular / Molecular Sequence Data / Point Mutation / Rabbits / Species Specificity / Streptococcus intermedius / Streptolysins (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15383705 ● CiNii @ National Institute of Informatics (CRID): 1521417754879669632 ● Search Scopus @ Elsevier (PMID): 15383705 (PubMed: 15383705, CiNii: 1521417754879669632)
55.	Taniguchi Kazuko, Tsuneko Ono, Keiji Murakami, Darija Viducic, Kayama Sizuo, Katsuhiko Hirota, Ken Nemoto and Yoichiro Miyake : Novel Pseudomonas aeruginosa gene that suppresses tolerance to carbapenems, Antimicrobial Agents and Chemotherapy, Vol.47, No.9, 2997-3001, 2003. (Summary) A biapenem-tolerant mutant of Pseudomonas aeruginosa was isolated by Tn1737KH insertion. The survival of the mutant 3 h after the addition of biapenem was about 1000 times greater than that of the wild type. The mutant was also tolerant to other biapenems, such as imipenem, panipenem, and meropenem. (Keyword) Animals / Carbapenems / Colony Count, Microbial / DNA Primers / DNA Transposable Elements / Drug Resistance, Bacterial / Mice / knockout mice / Microbial Sensitivity Tests / Plasmids / Pseudomonas aeruginosa (Link to Publication Site) ● Publication site (DOI): 10.1128/AAC.47.9.2997-3001.2003 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12937012 ● Search Scopus @ Elsevier (PMID): 12937012 ● Search Scopus @ Elsevier (DOI): 10.1128/AAC.47.9.2997-3001.2003 (DOI: 10.1128/AAC.47.9.2997-3001.2003, PubMed: 12937012)
56.	Atsushi Tabata, Hideaki Nagamune, Takuya Maeda, Keiji Murakami, Yoichiro Miyake and Hiroki Kourai : Correlation between resistance of Pseudomonas aeruginosa to quaternary ammonium compounds and expression of outer membrane protein OprR, Antimicrobial Agents and Chemotherapy, Vol.47, No.7, 2093-2099, 2003. (Summary) 論文題目:緑膿菌の細胞膜タンパク質OprRの発現と第四アンモニウム塩に対する耐性化の相関第四アンモニウム塩系消毒剤に耐性化した緑膿菌において顕著に発現量が増加する約25.6kDaの蛋白質を2次元電気泳動で分離し，そのN末端アミノ酸配列の解析によって，それが外膜蛋白質のOprRであることを明らかとした．またその耐性化株でOprRのノックアウト株を作成したところ，第四アンモニウム塩系消毒剤に対する感受性が野生株と同程度まで上昇することから，OprRがこの消毒薬耐性に重要な役割を果たしていることを示した． (Keyword) Adaptation, Physiological / Amino Acid Sequence / Bacterial Outer Membrane Proteins / Drug Resistance, Bacterial / Gene Deletion / Genes, Bacterial / Molecular Sequence Data / Mutagenesis / Pseudomonas aeruginosa / Quaternary Ammonium Compounds (Tokushima University Institutional Repository) ● Metadata: 117786 (Link to Publication Site) ● Publication site (DOI): 10.1128/AAC.47.7.2093-2099.2003 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12821452 ● Search Scopus @ Elsevier (PMID): 12821452 ● Search Scopus @ Elsevier (DOI): 10.1128/AAC.47.7.2093-2099.2003 (Tokushima University Institutional Repository: 117786, DOI: 10.1128/AAC.47.7.2093-2099.2003, PubMed: 12821452)
57.	Ken Nemoto, Katsuhiko Hirota, Keiji Murakami, Kazuko Taniguti, Hiromi Murata, Darija Viducic and Yoichiro Miyake : Effect of varidase (streptodornase) on biofilm formed by Pseudomonas aeruginosa, Chemotherapy, Vol.49, No.3, 121-125, 2003. (Summary) The biofilm of Pseudomonas aeruginosa could be removed by Varidase (streptodornase) that was used as defibrinating drug. After cultivating the biofilm on a silicone chip in a modified alginate-producing medium in vitro, it was treated with Varidase or DNase I. In both treatments, the biofilm was removed depending on the concentration of the reagents. Varidase was apparently effective under the condition that the biofilm was exposed to more than 625 U/ml (a quarter of the concentration of the medical use) for 3 h, twice, at 37 degrees C. The result of this experiment indicates that (a) the DNases, DNase I and Varidase, were effective in destroying the biofilm of P. aeruginosa in vitro, and (b) in a clinical field, Varidase could be useful for P. aeruginosa focal infection, such as urinary tract infection, by removing the biofilm. (Keyword) Alginates / Biofilms / Cell Culture Techniques / Culture Media / Deoxyribonuclease I / Dose-Response Relationship, Drug / Humans / Pseudomonas Infections / Pseudomonas aeruginosa / Urinary Tract Infections (Link to Publication Site) ● Publication site (DOI): 10.1159/000070617 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12815204 ● Summary page in Scopus @ Elsevier: 2-s2.0-0038647338 (DOI: 10.1159/000070617, PubMed: 12815204, Elsevier: Scopus)
58.	奥井学, 日比野豊, Katsuhiko Hirota, Mizuho Nishino, Yoichiro Miyake and 粟津邦男 : PBIIでフッ素イオン注入した口腔内装置用材料の細菌付着抑制効果に関する研究, 生体材料, Vol.20, No.2, 55-62, 2002. (Keyword) fluorine / plasma / implantation / bacteria / titanium (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520009409707241344 (CiNii: 1520009409707241344)
59.	Takatsugu Goto, Hideaki Nagamune, Aiko Miyazaki, Yoshiaki Kawamura, Ooki Ohnishi, Kanako Hattori, Kazuto Ohkura, Kazuaki Miyamoto, Shigeru Akimoto, Takayuki Ezaki, Katsuhiko Hirota, Yoichiro Miyake, Takuya Maeda and Hiroki Kourai : Rapid identification of Streptococcus intermedius by PCR with the ily gene as a species marker gene, Journal of Medical Microbiology, Vol.51, No.2, 178-186, 2002. (Summary) ヒト特異的細胞溶解毒素インターメディリシン(ILY)遺伝子はアンギノーサス群連鎖球菌ではS. intermedius(SI)のみに特異的に存在したことから，ily遺伝子が本菌種にだけ分布する可能性が考えられた．そこでPCRやサザンドットハイブリダイゼーション法を用いて，連鎖球菌属や近縁菌種におけるily遺伝子の分布を調べ，ily遺伝子がSI特異的な菌種マーカーになり得ることを確認し，それを指標として本菌種による感染症診断に応用可能なPCR検出システムを構築した． (Keyword) MILLERI GROUP / ANGINOSUS GROUP / LIVER-ABSCESS / CONSTELLATUS / STRAINS / SYSTEM / HYBRIDIZATION (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11863269 ● Search Scopus @ Elsevier (PMID): 11863269 (PubMed: 11863269)
60.	Yoko Terada, Katsuhiko Hirota, Toshiya Kashiwabara, Kan Nagao, Yoichiro Miyake and Tetsuo Ichikawa : Identification of Helicobacter Pylori in Denture Plaque of Hospitalized Aged Patients, Dentistry in Japan, Vol.37, 56-58, 2001. (Keyword) Helicobacter Pylori / Denture Plaque / PCR / Oral Microorganism
61.	Sizuo Kitano, Kenji Irimura, Toru Sasaki, Naoko Abe, Atsuyo Baba, Yoichiro Miyake, Nobuhiko Katunuma and Kenji Yamamoto : Suppression of gingival inflammation induced by Porphyromonas gingivalis in rats by leupeptin, The Japanese Journal of Pharmacology, Vol.85, No.1, 84-91, 2001. (Summary) In this study, we developed a procedure to produce gingivitis in rats by inoculation of Porphyromonas gingivalis and studied the contribution of the bacterial cysteine proteinases, Arg-gingipain (Rgp) and Lys-gingipain (Kgp), to the pathology in the gingiva. To adhere the bacterium to periodontal tissues, a cotton thread was inserted between the first and second molar of right maxillary sites of rats. Rats in group A were administered with vehicle alone after bacterial (strain W83) inoculation. In group B, the bacteria were inoculated in combination with leupeptin, a potent inhibitor of Rgp and Kgp, and then leupeptin alone was administered the week after. Rats in group C were administered leupeptin for 6 weeks after bacteria inoculation. All left maxillary gingiva in three groups showed no inflammatory changes. Right maxillary gingiva of group A showed most of the clinical landmarks of gingivitis. Leupeptin exhibited only a little inhibitory effect on this gingivitis in group B, whereas it had a strong inhibitory effect on the inflammation in group C. These results suggest that P. gingivalis-induced gingivitis is attributable to Rgp and Kgp and that leupeptin is more effective in the late phase than the early stage of gingivitis. (Keyword) Adhesins, Bacterial / Animals / Bacteroidaceae Infections / Cysteine Endopeptidases / Cysteine Proteinase Inhibitors / Disease Models, Animal / Disease Progression / Gingipain Cysteine Endopeptidases / Gingivitis / Hemagglutinins / Leupeptins / male / Maxilla / Porphyromonas gingivalis / Rats / Rats, Wistar / Virulence (Link to Publication Site) ● Publication site (DOI): 10.1254/jjp.85.84 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11243579 ● Summary page in Scopus @ Elsevier: 2-s2.0-0035135389 (DOI: 10.1254/jjp.85.84, PubMed: 11243579, Elsevier: Scopus)
62.	Tsuneko Ono, Sumiko Shiota, Katsuhiko Hirota, Ken Nemoto, Tomofusa Tsuchiya and Yoichiro Miyake : Susceptibilities of Oral and Nasal Isolates of Streptococcus mitis and Streptococcus oralis to Macrolides and PCR Detection of Resistance Genes, Antimicrobial Agents and Chemotherapy, Vol.44, No.4, 1078-1080, 2000. (Summary) The susceptibility of viridans group streptococci to macrolides was determined. Thirteen isolates (17%) were resistant to erythromycin. Five strains carried an erm gene that was highly homologous to that in Tn917. Four strains had mefE genes that coded erythromycin efflux ability. (Keyword) Anti-Bacterial Agents / Base Sequence / Carrier Proteins / DNA, Bacterial / DNA-Binding Proteins / Drug Resistance, Microbial / Erythromycin / Genes, Bacterial / Methyltransferases / Microbial Sensitivity Tests / Molecular Sequence Data / Mouth / Nasal Cavity / Reverse Transcriptase Polymerase Chain Reaction / Streptococcus / Streptococcus oralis / Transcription Factors (Link to Publication Site) ● Publication site (DOI): 10.1128/AAC.44.4.1078-1080.2000 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10722517 ● Summary page in Scopus @ Elsevier: 2-s2.0-0034018955 (DOI: 10.1128/AAC.44.4.1078-1080.2000, PubMed: 10722517, Elsevier: Scopus)
63.	Kinya Murakami, Katsuhiko Hirota, Takashi Matsuo and Yoichiro Miyake : Effect of antibiotics on CD15s-related antigen expressed on the Streptococcus anginosus group, Dentistry in Japan, Vol.36, 22-27, 2000. (Keyword) S. Anginosus Group / CD15s-Related Antigen / antibiotics (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1570291224404669952 (CiNii: 1570291224404669952)
64.	Ken Nemoto, Katsuhiko Hirota, Tsuneko Ono, Kinya Murakami, Keiji Murakami, Daisuke Nagao and Yoichiro Miyake : Effect of varidase (streptokinase) on biofilm formed by Staphylococcus aureus, Chemotherapy, Vol.46, No.2, 111-115, 2000. (Summary) Staphylococcus aureus forms a fibrin-rich biofilm in the presence of plasma which is highly resistant to attack by the human immune system and to chemotherapy. Varidase, composed mainly of streptokinase, is used for hydrolyzing clots. In this study, we attempted to destroy the biofilm of S. aureus with Varidase and to apply this drug in the treatment of staphylococcal infections. Four clinical isolates were used in the experiments. These organisms formed a several-millimeter-thick biofilm on type IV collagen coated coverslips in trypticase soy broth containing 50% human plasma. The biofilm was composed of bacterial cell which adhered to fibrillar fibers and of sediment derived from plasma. 10,000 U/ml of Varidase, the dose which is used clinically, removed the sediment and reduced the number of live bacteria in biofilms to less than 20% of control. 200 U/ml of Varidase was also effective against biofilms of the organisms. An equal combination of Varidase and ofloxacin had an additive effect on the bacteria. The results of this study demonstrate that Varidase is highly effective in destroying biofilms of S. aureus in vitro and suggest that this drug would be useful for treating staphylococcal infections. (Keyword) Staphylococcus Aureus / Biofilm / Varidase / Ofloxacin / Resistance (Link to Publication Site) ● Publication site (DOI): 10.1159/000007264 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10671761 ● Summary page in Scopus @ Elsevier: 2-s2.0-0033953104 (DOI: 10.1159/000007264, PubMed: 10671761, Elsevier: Scopus)
65.	Katsuhiko Hirota, Hiromi Murata, Hiromichi Yumoto, Kazuto Ohkura, Hideaki Nagamune and Yoichiro Miyake : Three-dimensional structural analysis of histone-like protein from streptococci, Bacterial Adherence研究, Vol.14, 6-8, 2000.
66.	Tetsuo Ichikawa, Yoko Terada, Katsuhiko Hirota, Yoichiro Miyake and Kazuo Hosoi : Study on oral health care and general health of elderly (in Japanese), Dentistry in Japan, Vol.19, 75-80, 2000. (Keyword) 高齢者 / 口腔 / 咽頭細菌 / 唾液 / 舌
67.	Fumiko Michishige, Sumiko Yoshinaga, Eriko Harada, Katsuhiko Hirota, Yoichiro Miyake, Takashi Matsuo and Susumu Yasuoka : Relationships between activity of daily living, and oral cavity care and the number of oral cavity microorganisms in patients with cerebrovascular diseases, The Journal of Medical Investigation : JMI, Vol.46, No.1-2, 79-85, 1999. (Summary) We examined the relationships among the activity of daily living (ADL), oral cavity care, and the number of oral cavity microorganisms in 40 patients with cerebrovascular diseases (CVD). The CVD patients were classified into 4 groups, I, II, III and IV based on their ADL and the method used for oral cavity care. The ADL was highest in group I and lowest in group III. Only the patients of only group III could not eat by themselves and were receiving naso-esophageal feeding. Oral cavity care was performed by the patients themselves in groups I and IV, but was performed by caregivers in groups II and III. The group IV patients had no teeth, but could eat by themselves using full dentures. The numbers of microorganisms in the pharyngeal swabs from the 4 groups were measured and expressed as colony-forming units (cfu). The numbers of both Staphylococci spp. and Candida spp. were significantly higher in group III than in the other groups. Moreover, Pseudomonas aeruginosa was isolated only from patients of group III (in about 66%). The oral cavity care by caregivers was almost the same in groups II and III, but the numbers of oral cavity microorganisms were significantly higher in group III than in group II. These results indicated that microorganisms grow more easily in the oral cavities of CVD patients with low ADL compared with CVD patients with higher ADL, and that eating is thought to be important for the prevention of an increase of microorganisms in the oral cavity. (Keyword) Oral cavity care / Aspiration Pneumonia / cerebrovascular disease / Pharyngeal Swab / bacteria (Tokushima University Institutional Repository) ● Metadata: 22626 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10408162 ● Search Scopus @ Elsevier (PMID): 10408162 (Tokushima University Institutional Repository: 22626, PubMed: 10408162)
68.	Katsuhiko Hirota, Kinya Murakami, Ken Nemoto, Tsuneko Ono, Takashi Matsuo, Hiromi Kumon and Yoichiro Miyake : Fosfomycin reduces CD15s-related antigen expression of Streptococcus pyogenes, Antimicrobial Agents and Chemotherapy, Vol.42, No.5, 1083-1987, 1998. (Summary) We have previously shown the immunological mimicry of human sialyl-Lewis(x) (CD15s) by a surface antigen of Streptococcus pyogenes. This mimicking surface antigen may act as a ligand to the selectin family and may induce antibody production against CD15s on host cells, suggesting a possible role in the pathogenesis of S. pyogenes. In this study, the effects of antibiotics on the CD15s-related antigen expression of S. pyogenes were examined at a concentration below the MIC (sub-MIC). The amounts of CD15s on the surfaces of S. pyogenes cells and on the surfaces of S. pyogenes biofilms were determined by a whole-cell enzyme-linked immunosorbent assay and by laser scanning fluorescence microscopy, respectively, by using an anti-CD15s monoclonal antibody. At the sub-MICs, fosfomycin (1R,2S-1,2-epoxypropyl phosphonic acid), its enantiomer (1S,2R-1,2-epoxypropyl phosphonic acid), and benzylpenicillin significantly inhibited the CD15s expression of all strains studied. The effects of fosfomycin and its enantiomer on biofilms were also observed by scanning electron microscopy. Incubation of S. pyogenes with the sub-MIC of fosfomycin or its enantiomer, which has no antibacterial activity, reduced the amount of CD15s on the biofilm surface and made it smooth. These results suggest that fosfomycin or its enantiomer might be useful for preventing S. pyogenes adherence to human CD15s receptors and the resulting immunological pathogenicity. (Keyword) Anti-Bacterial Agents / Antigens, Bacterial / Biofilms / Fosfomycin / Humans / Lewis X Antigen / Microbial Sensitivity Tests / Microscopy, Electron, Scanning / Molecular Mimicry / Streptococcus pyogenes (Link to Publication Site) ● Publication site (DOI): 10.1128/AAC.42.5.1083 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9593131 ● Summary page in Scopus @ Elsevier: 2-s2.0-0031970340 (DOI: 10.1128/AAC.42.5.1083, PubMed: 9593131, Elsevier: Scopus)
69.	Seiji Fujiwara, Yoichiro Miyake, Tsuguru Usui and Hidekazu Suginaka : Effect of adherence on antimicrobial susceptivility of Pseudomonas aeruginosa, Serratia marcescens and {Proteus mirabilis}, Hiroshima Journal of Medical Sciences, Vol.47, No.1, 1-5, 1998. (Summary) A simple method was used for testing the antibiotic susceptibility of adherent bacteria to plastic surfaces. Pseudomonas aeruginosa, Serratia marcescens, and Proteus mirabilis cells adhering to the bottom of a plastic tissue culture plate were incubated in serially diluted antibiotic solutions. After 24-h incubation the solutions were removed and a fresh medium without antibiotics was added to each well. The viability of the cells was judged by their growth after a further 24-h incubation. In our assay system, we employed a short incubation time (1-h) involving adherence of bacteria to a surface for the purpose of minimizing the effect of the glycocalyx on antibiotic activity. Even if the bacteria did not form a biofilm, the minimal bactericidal concentrations for adherent bacteria (MBCADs) markedly elevated. The MBCADs of ofloxacin well correlated with the bacteriological eradication by ofloxacin treatment for urinary tract infections (UTIs) associated with indwelling urinary catheters, whereas the minimal inhibitory concentrations did not show a correlation. Kinetic studies showed that adherent Pseudomonas aeruginosa had a 2 h-lag time before logarithmically growing when these bacteria were incubated in Mueller-Hinton broth without antibiotics. The tolerance demonstrated by adherent cells is likely to play a role in the difficulties encountered in the antimicrobial chemotherapy of biofilm infections. Moreover, our assay system was considered to be useful in the therapeutic selection of antibiotics for these infections. (Keyword) Bacterial Adhesion / Biofilms / Drug Resistance, Microbial / Humans / Prosthesis-Related Infections / Proteus mirabilis / Pseudomonas aeruginosa / Serratia marcescens (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9583277 ● Summary page in Scopus @ Elsevier: 2-s2.0-0031920370 (PubMed: 9583277, Elsevier: Scopus)
70.	Tetsuo Ichikawa, Katsuhiko Hirota, H Kanitani, Yoichiro Miyake and N Matsumoto : In vitro adherence of Streptococcus constellatus to dense hydroxyapatite and titanium, Journal of Oral Rehabilitation, Vol.25, No.2, 125-127, 1998. (Summary) Bacterial adherence to dense hydroxyapatite (HA) and titanium (Ti) rods was examined in vitro using Streptococcus constellatus ATCC27832. Streptoccus constellatus is associated with dental abscess and was isolated from the peri-implantitis. The dense HA was etched with 5% hydrofluoric acid for 0, 10, 20, 30, 40, and 50 s Adherence to the untreated dense HA was twice as high as the adherence to the Ti. The adherence to saliva-treated Ti was 13% higher than that to untreated Ti. The adherence was increased by acid-treatment of the dense HA, following a sigmoid curve from 20 to 50 s of treatment time. The dense HA has the capacity to receive more bacterial adherence than Ti and furthermore, the etched dense HA becomes more adherent. (Keyword) Abscess / Acid Etching, Dental / analysis of variance / Bacterial Adhesion / Dental Implants / Durapatite / Humans / Hydrofluoric Acid / periodontitis / Saliva / Streptococcal Infections / Streptococcus / surface properties / Time Factors / titanium / Tooth Diseases (Link to Publication Site) ● Publication site (DOI): 10.1046/j.1365-2842.1998.00216.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9576596 ● Summary page in Scopus @ Elsevier: 2-s2.0-0031994809 (DOI: 10.1046/j.1365-2842.1998.00216.x, PubMed: 9576596, Elsevier: Scopus)
71.	Junji Suzuki, Hitoshi Komatsuzawa, Motyoyuki Sugai, Takako Suzuki, Katsuyuki Kozai, Yoichiro Miyake, Hidekazu Suginaka and Nobuo Nagasaka : A long-term survey of methicillin-resistant Staphylococcus aureus in the oral cavity of children, Microbiology and Immunology, Vol.41, No.9, 681-686, 1997. (Summary) Methicillin-resistant Staphylococcus aureus (MRSA), an indigenous bacteria in healthy people, often causes nosocomial infections. If the host human becomes compromised, MRSA can cause a serious infection. The long-term colonization of MRSA increases this risk. The purpose of this study was to demonstrate the incidence of S. aureus and MRSA colonization in the oral cavities of healthy children, and to examine the stability of identical strains of MRSA over a long-term period. Fourteen children were examined in two stages (first stage: 1987-88, second stage: 1992-93). Five of the 14 children were negative for S. aureus in both stages, seven children were positive in both stages and two children were positive in only the second stage. The children who were colonized with S. aureus in the first stage always harbored the bacteria in the second stage. Of the seven children that were positive for S. aureus in both stages, three persisted in carrying MRSA. We compared two MRSA strains isolated from the same children in both stages by coagulase typing, antibiogram typing and DNA fingerprinting. In two children, the strains showed the same coagulase types, similar antibiograms and similar DNA fragment profiles. These data strongly suggest that identical strains of MRSA persisted in the oral cavities for more than five years, and that the oral cavity can serve as a reservoir for MRSA with the potential to cause nosocomial infections. (Keyword) Carrier State / Child / Child, Preschool / Disease Reservoirs / Humans / Japan / Methicillin Resistance / Mouth / Staphylococcal Infections / Staphylococcus aureus (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1348-0421.1997.tb01911.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9343818 ● Summary page in Scopus @ Elsevier: 2-s2.0-0030924110 (DOI: 10.1111/j.1348-0421.1997.tb01911.x, PubMed: 9343818, Elsevier: Scopus)
72.	Katsuhiko Hirota, 米山武義, 太田昌子, 橋本賢二 and Yoichiro Miyake : Pharyngeal bacteria and professional oral health care in elderly people, Japanese Journal of Geriatrics, Vol.34, No.2, 125-129, 1997. (Keyword) geriatric nursing / 咽頭細菌数 / プロフェッショナル·オーラル·ヘルス·ケア (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001205019891840 (CiNii: 1390001205019891840)
73.	米山武義, 相羽寿史, 太田昌子, Katsuhiko Hirota, Yoichiro Miyake, 橋本賢二 and 岡本浩 : Treatment of gingivitis in elderly patients in a nursing home, Japanese Journal of Geriatrics, Vol.34, No.2, 120-124, 1997. (Keyword) 高齢者 / 歯肉炎 / 歯垢付着 / 歯周疾患 / 特別養護老人ホーム (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001205019890432 (CiNii: 1390001205019890432)
74.	Tetsuo Ichikawa, Katsuhiko Hirota, Hideo Kanitani, Rudi Wigianto, Naeko Kawamoto, Naoyuki Matsumoto and Yoichiro Miyake : Rapid bone resorption adjacent to hydroxyapatite-coated implants, The Journal of Oral Implantology, Vol.22, No.3,4, 232-235, 1997. (Summary) This paper describes rapid bone resorption in the peri-implantitis of HA implants based on both our clinical observations of and histological research on extracted dense hydroxyapatite (HA) implants. The surfaces of extracted HA implants were rough, although they were smooth at fixture placement. Plaque formed on the necks of the implants, whereas little plaque was seen on the bottoms. The plaque consisted of cocci and rods, including filamentous bacteria. Few spirochetes were observed. Although surrounding bone was formed rapidly around the HA implant, bone thickness gradually decreased compared with the titanium implant. These facts suggest that the rigid biointegration of HA with the thin surrounding bone--that is, the overstressing of the bone--causes rapid bone resorption rather than plaque accumulation on HA. (Keyword) Hydroxyapatite / Peri-implantitis / Bone Resorption / Overstressing (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9524500 ● Search Scopus @ Elsevier (PMID): 9524500 (PubMed: 9524500)
75.	T. Matsuura, A. Kohada, T. Yamamoto, Yoichiro Miyake, Y. Akagawa, H. Suginaka and H. Tsuru : High incidence of Staphylococcus aureus] from dentures and tongues of maxillary resection patients, Oral Microbiology and Immunology, Vol.12, 354-357, 1997.
76.	Toshiya Kashiwabara, Tetsuo Ichikawa, Naeko Kawamoto, Hideo Kanitani, 堀内政信, Katsuhiko Hirota, Yoichiro Miyake and 松本直之 : 老人病院入院患者の口腔状態とデンチャープラークの細菌構成について, The Journal of the Japan Prosthodontic Society, Vol.40, No.3, 448-453, 1996. (Keyword) Elderly Patient / Edentulous / Geriatric / Denture Plaque / MRSA
77.	Naeko Kawamoto, Tetsuo Ichikawa, Hideo Kanitani, Toshiya Kashiwabara, 堀内政信, Katsuhiko Hirota, Yoichiro Miyake and 松本直之 : 電解水の義歯洗浄効果, The Journal of the Japan Prosthodontic Society, Vol.40, No.3, 574-579, 1996. (Keyword) Acidic Electrolyzed Water / Neutral Electrolyzed Water / Denture Cleanser / Denture Plaque
78.	Takashi Matsuura, Yoichiro Miyake, Seiji Nakashima, Hitoshi Komatsuzawa, Yasumasa Akagawa and Hidekazu Suginaka : Isolation and characterization of teichoic acid-like substance as an adhesin of Staphylococcus aureus to HeLa cells, Microbiology and Immunology, Vol.40, No.4, 247-254, 1996. (Summary) A cell wall component that bound to HeLa cells (HeLa cell-binding CWC) was isolated from a clinical isolate of Staphylococcus aureus. The HeLa cell-binding CWC was resistant to heat (100 C, 1 hr) and proteases, did not stain with Coomassie Brilliant Blue R-250 on SDS-PAGE but stained as a broad band with antiserum against the strain on Western blots. These data suggest that the HeLa cell-binding CWC is not a protein, and may be teichoic acid. Purified teichoic acid bound to HeLa cells, whereas fractions without teichoic acid did not. In Western blots, HeLa cell-binding CWC appeared as a broad band of less than 35 kDa, similar to that of purified teichoic acid. These data suggest that the HeLa cell-binding CWC obtained in this study is teichoic acid. Teichoic acid inhibited S. aureus adherence to HeLa cells and bound to the cells time and dose dependently, in a saturable and reversible manner, and therefore appears to be an adhesin of S. aureus to HeLa cells. (Keyword) Adhesins, Bacterial / Bacterial Adhesion / HeLa Cells / Humans / Kinetics / Staphylococcus aureus / Teichoic Acids (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1348-0421.1996.tb03341.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 8709859 ● Summary page in Scopus @ Elsevier: 2-s2.0-0029866396 (DOI: 10.1111/j.1348-0421.1996.tb03341.x, PubMed: 8709859, Elsevier: Scopus)
79.	Katsuhiko Hirota, Ro Osawa, Ken Nemoto, Tsuneko Ono and Yoichiro Miyake : Highly expressed human sialyl Lewis^x antigen on cell surface of Streptococcus gallolyticus, The Lancet, Vol.347, No.9003, 760, 1996. (Keyword) Animals / Antigens, Surface / Humans / Lewis X Antigen / Streptococcus bovis (Link to Publication Site) ● Publication site (DOI): 10.1016/s0140-6736(96)90109-9 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 8602016 ● Summary page in Scopus @ Elsevier: 2-s2.0-0029916909 (DOI: 10.1016/s0140-6736(96)90109-9, PubMed: 8602016, Elsevier: Scopus)
80.	Tetsuo Ichikawa, Katsuhiko Hirota, Hideo Kanitani, Wigianto Rudi, Naeko Kawamoto, 堀内政信, Yoichiro Miyake and 松本直之 : アパタイトインプラントの細菌付着に関する研究, The Journal of the Japan Prosthodontic Society, Vol.40, No.1, 40-45, 1996. (Keyword) Hydroxyapatite / implantation / Titanium / Bacteria Adherence / Streptococcus Constellatus
81.	Yoichiro Miyake, 杉中秀壽, 藤原政治 and 碓井亞 : In vitro antibacterial activity of pazufloxacin against bacteria adhering to solid phase, Japanese Journal of Chemotherapy, Vol.43, No.S-2, 66-71, 1995. (Keyword) 付着細菌 / 感受性 / PZFX / ニューキノロン (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282681263415040 (CiNii: 1390282681263415040)
82.	Ken Nemoto, Ro Osawa, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : An investigation of gram-negative tannin-protein complex degrading bacteria in fecal flora of various mammals, The Journal of Veterinary Medical Science, Vol.57, No.5, 921-926, 1995. (Summary) Gram-negative tannin-protein complex degrading bacteria (T-PCDB) were first isolated from animals except for the koala. The occurrence of T-PCDB in feces of 15 species of mammals with different feeding habits was investigated. T-PCDB occurred in 7 of 54 horses but they could not be isolated from other mammals tested. These T-PCDB comprised less than 0.1% of the facultative anaerobic microflora in horse feces and it was much less than that previously reported in koala feces ( > 60%). A total of 7 T-PCDB fecal isolates showed a range of phenotypic diversities. They were all Gram-negative rods of various sizes and shapes including coccoidal rod. Although all produced tannase, no strain showed to have gallate decarboxylase. A total of 23 representative strains belonging to the family Pasteurellaceae were also tested for tannase production. Two strains, Haemophilus actinomycetemcomitans NIAH-10202T and Haemophilus segnis NIAH-10183T which were isolated from human oral cavity were detected positive for tannase. (Keyword) Animals / Cats / Cattle / Dogs / Feces / Gram-Negative Bacteria / Gram-Negative Bacterial Infections / Guinea Pigs / Haemophilus / Horses / Humans / Macropodidae / Mammals / Mice / Opossums / Pasteurellaceae / Phenotype / Rabbits / Rats / Sheep / Swine / Tannins (Link to Publication Site) ● Publication site (DOI): 10.1292/jvms.57.921 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 8593303 ● Summary page in Scopus @ Elsevier: 2-s2.0-0029383654 (DOI: 10.1292/jvms.57.921, PubMed: 8593303, Elsevier: Scopus)
83.	Motoyuki Sugai, Hitoshi Komatsuzawa, Tomoko Akiyama, Yeong-Man Hong, Tadahiro Oshida, Yoichiro Miyake, Toutaro Yamaguchi and Hidekazu Suginaka : Identification of endo-β-N-acetylglucosaminidase and N-acetylmuramyl-L-alanine amidase as cluster-dispersing enzymes in Staphylococcus aureus, Journal of Bacteriology, Vol.177, No.6, 1491-1496, 1995. (Summary) Two proteins which are capable of dispersing cell clusters of Staphylococcus aureus have been purified from a S. aureus FDA209P culture supernatant. Both of them were found to have bacteriolytic activity. From the elution profile of column chromatography and Western blot (immunoblot) analysis, one of them was identified as a 51-kDa endo-beta-N-acetylglucosaminidase (GL). The other was a 62-kDa protein on the basis of sodium dodecyl sulfate gel electrophoresis. Analysis of the peptidoglycan fragments following treatment with the 62-kDa protein indicated that this protein is an N-acetylmuramyl-L-alanine amidase (AM). In vitro studies of cluster dispersion activities using S. aureus mutant strains Lyt66 or S. aureus Wood46 grown as clusters demonstrated that these two enzymes act synergistically to disperse clusters into single cells. Antiserum against the 51-kDa GL cross-reacted with the 62-kDa AM, and S. aureus FDA209P grown in the presence of anti-51-kDa-GL immunoglobulin G induced giant clusters. Clusters induced by anti-51-kDa GL and by Cibacron blue F3G-A were dispersed by coincubation with the 51-kDa GL and the 62-kDa AM. Western blot analysis demonstrated that the 51-kDa GL and the 62-kDa AM were missing in culture supernatants of S. aureus Lyt66, Wood46, and RUSAL2 (Tn551 autolysin-defective mutant), which grow in clusters. These results strongly suggest that the 51-kDa GL and 62-kDa AM are involved in cell separation of daughter cells after cell division. (Keyword) Acetylglucosaminidase / Bacterial Adhesion / Bacterial Proteins / Bacteriolysis / Blotting, Western / Mutagenesis, Insertional / Particle Size / Staphylococcus aureus (Link to Publication Site) ● Publication site (DOI): 10.1128/jb.177.6.1491-1496.1995 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 7883705 ● Summary page in Scopus @ Elsevier: 2-s2.0-0028986933 (DOI: 10.1128/jb.177.6.1491-1496.1995, PubMed: 7883705, Elsevier: Scopus)
84.	Katsuhiko Hirota, Hideo Kanitani, Ken Nemoto, Tsuneko Ono and Yoichiro Miyake : Cross-reactivity between human sialyl Lewis^x oligosaccharide and common causative oral bacteria of infective endocarditis, FEMS Immunology and Medical Microbiology, Vol.12, No.2, 159-164, 1995. (Summary) The expression of sialy-Lewis(x) (sLe(x); Neu5Ac alpha 2-3 Gal beta 1-4) (Fuc alpha 1-3) GlcNAc-R) on oral bacteria producing infective endocarditis was determined by a whole-cell enzyme linked immunosorbent assay and an immunoelectron microscopy using the well-characterized anti-sLe(x) monoclonal antibody SNH-3 (mAb SNH-3; IgM class). mAb SNH-3 reacted strongly with whole cells of oral bacteria: Streptococcus sanguis, Streptococcus mutans, Streptococcus mitis, Streptococcus salivarius, Streptococcus intermedius, Streptococcus constellatus, Streptococcus anginosus, Streptococcus pyogenes, Actinobacillus actinomycetemcomitans, Eikenella corrodens and Porphyromonas gingivalis. The negatively stained immuno-electron micrograph of Streptococcus pyogenes showed many reactive gold particles on the cell surface. Our findings demonstrated the existence of immunologic mimicry between the sLe(x) oligosaccharide and cell surface antigens of many species associated with infective endocarditis. We propose the hypothesis that if these bacteria escape their normal habitats, the surface components that mimic the sLe(x) oligosaccharide might bind to host antigens of the selectin family which could promote binding to endothelial cells and, consequently, initiation of the events leading to infective endocarditis. (Keyword) Sialyl Lewis^x / Infective Endocarditis / Oral Bacteria (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1574-695X.1995.tb00188.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 8589666 ● Search Scopus @ Elsevier (PMID): 8589666 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1574-695X.1995.tb00188.x (DOI: 10.1111/j.1574-695X.1995.tb00188.x, PubMed: 8589666)
85.	Yoichiro Miyake, K Tsuruda, K Okuda, (名) Widowati, Y Iwamoto and H Suginaka : In vitro activity of tetracyclines, macrolides, quinolones, clindamycin and metronidazole against periodontopathic bacteria, Journal of Periodontal Research, Vol.30, No.4, 290-293, 1995. (Summary) We re-evaluated several antibiotics including newer ones, for their in vitro killing activity, as well as their inhibitory activity, against clinical isolates of periodontopathic bacteria. Tetracyclines were active against Porphyromonas gingivalis, and were highly active against Prevotella intermedia, but demonstrated only a low killing activity against Actinobacillus actinomycetemcomitans. Rokitamycin, a new macrolide, and clindamycin were highly active against P. gingivalis and P. intermedia, but showed very weak killing activity against A. actinomycetemcomitans. Quinolones demonstrated excellent bactericidal activity against A. actinomycetemcomitans, and good inhibitory and bactericidal activity against P. gingivalis and P. intermedia. Metronidazole had an activity almost equivalent to quinolones against P. gingivalis and P. intermedia; but it was the least active against A. actinomycetemcomitans. (Keyword) Aggregatibacter actinomycetemcomitans / Anti-Bacterial Agents / Anti-Infective Agents / Antitrichomonal Agents / Clindamycin / Erythromycin / Fluoroquinolones / Metronidazole / Microbial Sensitivity Tests / Minocycline / Miocamycin / Naphthyridines / Ofloxacin / Porphyromonas gingivalis / Prevotella intermedia / Quinolones / Tetracycline (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1600-0765.1995.tb02136.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 7562327 ● Summary page in Scopus @ Elsevier: 2-s2.0-0029335379 (DOI: 10.1111/j.1600-0765.1995.tb02136.x, PubMed: 7562327, Elsevier: Scopus)
86.	Keiko Tsuruda, Yoichiro Miyake, Hidekazu Suginaka, Hiroshi Okamoto and Yoshifumi Iwamoto : Mirobiological features of gingivitis in pubertal children, Journal of Clinical Periodontology, Vol.22, No.4, 316-320, 1995. (Summary) The subgingival microflora of 42 pubertal children (aged 12-15 years) and 18 young adults (aged 21-25 years) was investigated by anaerobic culture and phase contrast microscopy. Motile rods, spirochetes and Prevotella intermedia were elevated proportionately in pubertal children with gingivitis (median GI > or = 1); however, no statistically significant differences in enumerated organisms on selective media were observed between pubertal children and young adults. These organisms were positively correlated with the index of bleeding on probing and the gingival index of pubertal children. Pubertal children were divided into 3 distinct subject clusters according to the similarity of subgingival microbial features, and the subjects who were then selected for the gingivitis group were distributed into clusters 1 and 2. The proportions of motile rods, P. intermedia and Eikenella corrodens were significantly higher in cluster 2 than in cluster 1. These results suggest that these 2 species and motile rods which differentiated features of clusters, are useful for screening of high-risk subjects for worsening of inflammation. (Keyword) Actinomyces / Adolescent / adult / bacteria / Bacteria, Anaerobic / children / Eikenella corrodens / female / Fusobacterium / Gingival Hemorrhage / Gingival Pocket / Gingivitis / Humans / male / Microscopy, Phase-Contrast / Periodontal Index / Prevotella intermedia / Puberty / Spirochaetales (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1600-051x.1995.tb00154.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 7622639 ● Summary page in Scopus @ Elsevier: 2-s2.0-0029283743 (DOI: 10.1111/j.1600-051x.1995.tb00154.x, PubMed: 7622639, Elsevier: Scopus)
87.	Motoyuki Sugai, Hitosi Komatsuzawa, Kaoru Ooku-Inomata, Yoichiro Miyake, Eisaku Ishida and Hidekazu Suginaka : Isolation and characterization Staphylococcus aureus mutans which form altered cell clusters, Microbiology and Immunology, Vol.38, No.12, 995-999, 1994. (Summary) Staphylococcus aureus FDA 209P produces two extracellular bacteriolytic enzymes, 51-kDa endo-beta-N-acetylglucosaminidase (GL) and 62-kDa N-acetylmuramyl-L-alanine amidase (AM), both of which can disperse cell clusters. To characterize the physiological roles of these enzymes in vivo, mutants with altered autolysin activity were isolated, and their degree of cluster formation in broth culture was assessed. Bacteriolytic activities of GL and AM, produced and secreted from these mutants into the culture fluid and detected with activity gels, coincided well with the degree of cluster formation of the mutants. The mutants with little or no enzyme activity grew in clusters, whereas those with high activity grew as well-separated cocci, suggesting that these enzymes are involved in cell separation of S. aureus in vivo. (Keyword) Acetylglucosaminidase / Muramidase / N-Acetylmuramoyl-L-alanine Amidase / Staphylococcus aureus (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1348-0421.1994.tb02158.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 7723694 ● Summary page in Scopus @ Elsevier: 2-s2.0-0028598581 (DOI: 10.1111/j.1348-0421.1994.tb02158.x, PubMed: 7723694, Elsevier: Scopus)
88.	Yoichiro Miyake, 藤原政治, 碓井亞 and 杉中秀壽 : In vitro antibacterial activity of biapenem against adherent bactera, Chemotherapy, Vol.42, No.S-4, 82-90, 1994. (Keyword) カルバペネム / 抗菌力

Academic Paper (Unrefereed Paper):

1.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota, 公文裕巳 and Yoichiro Miyake : 緑膿菌の抗菌薬抵抗性に対する新規化合物の作用機序, Bacterial Adherence & Biofilm, Vol.31, 2018.
2.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota, 公文裕巳 and Yoichiro Miyake : 新規化合物によるレボフロキサシン及びトブラマイシン殺菌作用の増強効果, Bacterial Adherence & Biofilm, Vol.30, 2017.
3.	Katsuhiko Hirota, Hiromichi Yumoto, Keiji Murakami, Kouji Hirao, Takashi Amoh and Yoichiro Miyake : Streptococcus intermedius biofilm componentsの糖鎖結合能と炎症反応誘導能に関する解析, Bacterial Adherence & Biofilm, Vol.29, 2016.
4.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota, 公文裕巳 and Yoichiro Miyake : 新規化合物によるビアペネム殺菌作用の増強効果について, Bacterial Adherence & Biofilm, Vol.29, 2016.
5.	Keiji Murakami, Takashi Amoh, Katsuhiko Hirota and Yoichiro Miyake : クラリスロマイシンによる緑膿菌c-di-GMPの誘導, 第50回緑膿菌感染症研究会講演記録 /緑膿菌感染症研究会事務局編, 2016.
6.	Katsuhiko Hirota, Hiromichi Yumoto, Yumi Hoshimo, Keiji Murakami, 宮本幸治, Takashi Matsuo and Yoichiro Miyake : 水溶性MPCポリマーの口腔細菌付着抑制効果, Bacterial Adherence & Biofilm, Vol.24, 47-49, 2011. (Summary) 口腔細菌によるbiofilm，すなわちdental plaqueの形成にはいくつかのkey stepがあり，dental plaque形成の抑制のためにはそれらのkey stepに効果的に働く方法を選択する必要が有る．我々は，超親水性表面を得ることができる2-methacryloloxethyl phosphorylcholine(MPC)co-polymerを用いてプラスチック表面をコートした界面は微生物の付着抑制に関与することを報告してきた．本実験では5%水溶性MPCポリマーで処理したHydroxyapatite(HY)プレートと培養歯肉上皮細胞(RT-7)へのStreptococcus mutansの付着状況を顕微鏡下にて観察した．その結果，5%水溶性MPCポリマー処理でHYプレート及び培養歯肉上皮細胞上へのS.mutansの付着は抑制されることが示された．以上よりHYプレートや培養歯肉上皮細胞に吸着した水溶性MPCポリマーは，プラスチックへのコーティングと同様にS.mutansの付着抑制効果を発揮することが示唆された．
7.	Keiji Murakami and Yoichiro Miyake : 緑膿菌臨床分離株におけるRSCVsの分離とその性状について, Bacterial Adherence & Biofilm, Vol.24, 81-83, 2011. (Summary) 緑膿菌感染症において，寒天培地上で小さな皺上のコロニー性状を示す，rugose small colony variants(RSCVs)の存在が報告されている．このRSCVsは，細胞内のセカンドメッセンジャーであるc-di-GMPの細胞内濃度が上昇しているためであると考えられている．今回我々は，徳島大学病院で分離された緑膿菌30株についてRSCVsの有無を検討したところ，2株が小さな皺状のコロニー性状を示し，バイオフィルム形性能が高く，運動性も低下していたことから，RSCVsであると考えられる．そのうちの1株は，c-di-GMP合成に関与する遺伝子オペロンの1つであるwspF遺伝子の変異の結果である事が明らかとなった．もう1株では，wspF遺伝子には大きな変異は見られなかった．これらの結果から，臨床分離株30株中2株のRSCVsが確認されたものの，その原因となる遺伝子は異なっていることが明らかとなった．
8.	Shizuo Kayama, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : The effect of culture supernatants of intestinal flora bacteria on the biofilm formation, Bacterial Adherence & Biofilm, Vol.21, 49-53, 2008.
9.	Shizuo Kayama, Keiji Murakami, Katsuhiko Hirota, Tsuneko Ono, Hideaki Nakae and Yoichiro Miyake : 付着緑膿菌の抗菌薬抵抗性とQuorum sensing機構について, Bacterial Adherence & Biofilm, Vol.20, 110-115, 2006. (Keyword) PPseudomonas aeruginosa / Quorum sensing system / Antibiotic tolerance / Biapenem
10.	Katsuhiko Hirota, Keiji Murakami, 鹿山鎮男, Ken Nemoto and Yoichiro Miyake : 微生物付着に与える2-methacryloyloxyethyl phosphorylcholine co-polymer処理の影響, Bacterial Adherence & Biofilm, Vol.17, 12-15, 2003. (Keyword) 2-methacryloyloxyethyl phosphorylcholine co-polymer / Superhydrophilicity / Microbial Adherence
11.	Tetsuo Ichikawa, Naoki Omura, 蟹谷容子, Katsuhiko Hirota and Yoichiro Miyake : デンタルデバイス感染症, Dental Outlook, Vol.99, No.2, 407-416, 2002.
12.	Katsuhiko Hirota, Keiji Murakami, Ken Nemoto and Yoichiro Miyake : Streptococcus intermediusの付着，凝集時におけるhistone-like proteinの局在, Bacterial Adherence & Biofilm, Vol.16, 27-32, 2002. (Keyword) tone-like protein / Localization / Streptococcus Intermedeus / Adherence
13.	Naoki Omura, Tetsuo Ichikawa, Katsuhiko Hirota and Yoichiro Miyake : デンチャープラークの最前線, デンタルエコー, Vol.122, 31-35, 2001.
14.	寺田容子, Tetsuo Ichikawa, Katsuhiko Hirota and Yoichiro Miyake : 要介護高齢者における咽頭微生物叢について, 私の研究から, Vol.691, 9-11, 2000.
15.	Fumiko Michishige, Sumiko Yoshinaga, Toshiko Terao, 原田江梨子, 安岡劭, Katsuhiko Hirota, Yoichiro Miyake, Toshiya Kashiwabara, Tetsuo Ichikawa and Takashi Matsuo : 含嗽の咽頭ぬぐい液細菌数に対する影響, Bulletin of School of Medical Sciences, the University of Tokushima, Vol.7, 35-42, 1997. (Keyword) 嚥下性肺炎 / 含嗽 / 咽頭ぬぐい液細菌数 / レンサ球菌
16.	Hitoshi Komatsuzawa, Junji Suzuki, Motoyuki Sugai, Yoichiro Miyake and Hidekazu Suginaka : The effect of Triton X-100 on the in-vitro susceptivility of mehicillin-resistant Staphylococcus aureus ot oxacillin, The Journal of Antimicrobial Chemotherapy, Vol.34, No.6, 885-897, 1994. (Summary) The effect of the non-ionic detergent, Triton X-100, on the in-vitro activity of oxacillin against methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains of Staphylococcus aureus was investigated. In the presence of Triton X-100, the MICs of oxacillin for both MRSA and MSSA isolates were reduced; this enhancing effect was particularly marked for the MRSA strains. Triton X-100 therefore counteracted the resistance to methicillin encoded by mecA. In the presence of oxacillin at subinhibitory concentrations, Triton X-100 induced the bacteriolysis of MRSA and potentiated the autolysis of these organisms. However, the detergent had no effect on the bacteriolytic enzyme profile or the susceptibility of the bacterial cell wall to bacteriolytic enzymes, nor did it promote the binding of oxacillin to the penicillin-binding protein (PBP) 2A. On the other hand, it stimulated the release from the bacteria of acylated lipoteichoic acid (LTA), a putative endogenous regulator of autolysins. Autolytic enzyme-deficient MRSA mutants were equally as sensitive as the parent strain to the effect of Triton X-100 on susceptibility to oxacillin. These results indicate that the enhanced in-vitro activity of oxacillin against MRSA in the presence of Triton X-100 cannot be accounted for simply by the induction of bacteriolysis following activation of autolytic enzymes by the detergent-stimulated release of LTA. (Keyword) Autolysis / cell death / cell division / Drug Interactions / Lipopolysaccharides / Methicillin Resistance / Mutagenesis / Octoxynol / Oxacillin / Staphylococcus aureus / Teichoic Acids (Link to Publication Site) ● Publication site (DOI): 10.1093/jac/34.6.885 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 7730232 ● Summary page in Scopus @ Elsevier: 2-s2.0-0028580156 (DOI: 10.1093/jac/34.6.885, PubMed: 7730232, Elsevier: Scopus)

Academic Letter:

1.	Yoichiro Miyake and Katsuhiko Hirota : 細菌学的に見た細菌カウンタの有用性, Dental Friends, Vol.9, 5-6, 2013.
2.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake : Biological Roles of Extracellular DNA in Bacterial Infection, Asean Plus and Tokushima Joint International Conference, Program and Proceeding Book, 47-48, 2012.
3.	Katsuhiko Hirota, Takeyoshi Yoneyama, Mayumi Sakamoto, Hiroshi Miyamoto, Masanori Kurihara, Shizuo Kayama, Keiji Murakami, Hiromichi Yumoto, Takashi Matsuo and Yoichiro Miyake : High prevalence of Pseudomonas aeruginosa from oropharyngeal biofilm in patients with cerebrovascular infarction and dysphagia., Chest, Vol.138, No.1, 237-238, 2010. (Keyword) Aged / Biofilms / Cerebral Infarction / Deglutition Disorders / female / Humans / male / Oropharynx / Pneumonia, Aspiration / Prevalence / Pseudomonas Infections / Pseudomonas aeruginosa (Link to Publication Site) ● Publication site (DOI): 10.1378/chest.10-0240 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20605833 ● Search Scopus @ Elsevier (PMID): 20605833 ● Search Scopus @ Elsevier (DOI): 10.1378/chest.10-0240 (DOI: 10.1378/chest.10-0240, PubMed: 20605833)
4.	清宮明香, Chieko Matsuura, Shizuo Kayama, Yoichiro Miyake, 田尾知浩, 吉川和寛, 土田健司, 水口潤, 川島周 and Tsuneko Ono : 透析液中の細菌検出における遺伝学的検討, 腎と透析65巻別冊ハイパフォーマンスメンブレン'08, 2008.
5.	Hideaki Nagamune, Akiko Sukeno, Robert A. Whiley, Syed I. Jafar, Joseph Aduse-Opoku, Kazuto Ohkura, Katsuhiko Hirota, Yoichiro Miyake, Takuya Maeda and Hiroki Kourai : Intermedilysin plays a key role in the invasion of human cells by Streptococcus intermedius, Streptococci New Insights into an Old Enemy: International Congress Series, Vol.1289, 262-266, 2006. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.ics.2005.11.021 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-33645897338 (DOI: 10.1016/j.ics.2005.11.021, Elsevier: Scopus)

Review, Commentary:

1.	Hiromichi Yumoto, Katsuhiko Hirota, Yoichiro Miyake, Keiji Murakami, Natsumi Fujiwara, 秋月皆人 and Hideki Fujii : ``撥菌''∼口腔バイオフィルム感染症予防への応用∼, The Journal of Educational Conference on All Japan Colleges of Dental Hygiene, Vol.10, No.25, 13-17, Mar. 2021. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1521980706066501120 (CiNii: 1521980706066501120)
2.	Keiji Murakami and Yoichiro Miyake : バイオフィルム形成菌の抗菌薬抵抗性「特集 ''One Health''時代の耐性菌対策・研究, Antibiotics and Chemotherapy, Vol.33, No.5, 1024-1028, May 2017.
3.	Yasusei Kudo, Tada Hidesuke, Natsumi Fujiwara, Tada Yoshiko, Takaaki Tsunematsu, Yoichiro Miyake and Naozumi Ishimaru : Oral environment and cancer, Genes and Environment, Vol.38, No.13, Aug. 2016. (Summary) Cancer is now the leading cause of death in Japan. A rapid increase in cancer mortality is expected as Japan is facing a super-aged society. Many causes of cancer are known to be closely linked to life style factors, such as smoking, drinking, and diet. The oral environment is known to be involved in the pathogenesis and development of various diseases such as bronchitis, pneumonia, diabetes, heart disease, and dementia. Because the oral cavity acts as the bodily entrance for air and food, it is constantly exposed to foreign substances, including bacteria and viruses. A large number of bacteria are endemic to the oral cavity, and indigenous oral flora act to prevent the settlement of foreign bacteria. The oral environment is influenced by local factors, including dental plaque, tartar, teeth alignment, occlusion, an incompatible prosthesis, and bad lifestyle habits, and systemic factors, including smoking, consumption of alcohol, irregular lifestyle and eating habits, obesity, stress, hormones, and heredity. It has recently been revealed that the oral environment is associated with cancer. In particular, commensal bacteria in the oral cavity are involved in the development of cancer. Moreover, Candida, human papilloma virus and Epstein-Barr virus as well as commensal bacteria have been reported to be associated with the pathogenesis of cancer. In this review, we introduce recent findings of the correlation between the oral environment and cancer. (Keyword) cancer / Oral environment / bacteria / Candida / Human papilloma virus / Epstein-Barr virus (Tokushima University Institutional Repository) ● Metadata: 114519 (Link to Publication Site) ● Publication site (DOI): 10.1186/s41021-016-0042-z (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27482300 ● Search Scopus @ Elsevier (PMID): 27482300 ● Search Scopus @ Elsevier (DOI): 10.1186/s41021-016-0042-z (Tokushima University Institutional Repository: 114519, DOI: 10.1186/s41021-016-0042-z, PubMed: 27482300)
4.	Yoichiro Miyake and Keiji Murakami : 付着緑膿菌の抗菌薬抵抗性, Antibiotics and Chemotherapy, Vol.31, No.9, 120-125, 2015.
5.	Keiji Murakami and Yoichiro Miyake : Regulation for bacterial function by macrolide, Respiratory Medicine, Vol.24, No.4, 397-399, Oct. 2013. (Keyword) macrolide / Pseudomonas aeruginosa / quorum sensing / virulence / biofilm (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520291856152869248 (CiNii: 1520291856152869248)
6.	Yoichiro Miyake : 口腔細菌のトランスロケーションによる感染症とその予防, Bacterial Adherence & Biofilm, Vol.26, 9-12, May 2013.
7.	Tsuneko Ono, Keiji Murakami and Yoichiro Miyake : Regulatory networks for antibiotic tolerance and biofilm formation in Pseudomonas aeruginosa, Nihon Saikingaku Zasshi, Vol.67, No.2, 227-243, May 2012. (Summary) <i>Pseudomonas aeruginosa</i>はcompromised hostにしばしば難治性慢性感染症を惹起する．その主な要因として，バイオフィルム形成能が高く抗菌薬に対する抵抗性が高いことが挙げられる．近年バイオフィルム形成のメカニズムについてはquorum sensing(QS)システム(密度感知機構)やcyclic di-guanosine monophosphate(c-di-GMP)等のグローバルレギュレーターの関与が明らかになり，また共焦点レーザー蛍光顕微鏡を用いたバイオフィルム内の遺伝子発現と形態的解析が進み，生理的性状の異なる多様な細胞集団で構成された多細胞生物的な構造を形成することが明らかになってきた．しかし抗菌薬抵抗性のメカニズムについては，まだその詳細は明らかではない．これまでに浮遊状態の細菌は，環境中の栄養源，pH，アルコール，浸透圧や菌体内代謝産物など各種のストレスを感知し，副次的ς因子やQSを介して，固相表面に付着しマイクロコロニーさらに菌体外マトリクスを形成することによりバイオフィルムへと移行し，各種の環境ストレスに対する抵抗性が上昇する．本稿では，<i>P. aeruginosa</i>のRpoS, RpoN等のストレス関連ς因子やQSおよびc-di-GMPなどのセカンドメッセンジャーと抗菌薬抵抗性との関連性について，<i>P. aeruginosa</i>のライフサイクルとの関わりを含めて，最近の知見を中心に概説する．<br> (Keyword) Animals / Anti-Bacterial Agents / Bacterial Adhesion / Bacterial Proteins / Biofilms / Cyclic GMP / Drug Resistance, Bacterial / Extracellular Matrix / Life Cycle Stages / Pseudomonas aeruginosa / Quorum Sensing / RNA Polymerase Sigma 54 / Second Messenger Systems / Sigma Factor (Link to Publication Site) ● Publication site (DOI): 10.3412/jsb.67.227 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22688178 ● CiNii @ National Institute of Informatics (CRID): 1390001206454468864 ● Search Scopus @ Elsevier (PMID): 22688178 ● Search Scopus @ Elsevier (DOI): 10.3412/jsb.67.227 (DOI: 10.3412/jsb.67.227, PubMed: 22688178, CiNii: 1390001206454468864)
8.	Yoichiro Miyake : 緑膿菌の抗菌薬抵抗性メカニズムの解明に向けて, BACTERIAL ADHERENCE & BIOFILM, Vol.24, 21-23, May 2011. (Summary) 抗菌薬抵抗性とは，高濃度の抗菌薬存在下で増殖はしないが，殺菌はされにくいという性状である．緑膿菌における抗菌薬抵抗性メカニズムについて，主にノックアウト変異株を用い現在までに以下のことが明らかになった．多糖体合成に関わるpslL遺伝子，rpoS遺伝子，ppGpp合成に関与するrelA遺伝子およびlas系遺伝子が抗菌薬抵抗性の上昇に関わっており，逆に機能不明のtcp遺伝子，ppGppの分解に関与しているといわれているspoTおよびdksA遺伝子は抗菌薬抵抗性の抑制に働いていた．さらに固層へ付着した細菌の抗菌薬抵抗性にはpslL遺伝子およびlas系遺伝子の関与が示唆され，細胞内のppGpp濃度が抵抗性に関わっていることも明らかになった．また，セカンド・メッセンジャーであるCyclic-di-GMPの濃度が緑膿菌の抗菌薬抵抗性において何らかの働きをしていることも示された．
9.	Yoichiro Miyake and Keiji Murakami : バイオフィルム形成とクオラムセンシング臨床医学編口腔内感染症とバイオフィルム, Antibiotics and Chemotherapy, Vol.26, No.9, 66-70, 2010.
10.	Shizuo Kayama and Yoichiro Miyake : 緑膿菌の抗菌薬抵抗性に影響を与える因子について, Shikoku Dental Research, Vol.21, No.2, 421-422, Mar. 2009. (Tokushima University Institutional Repository) ● Metadata: 109786 (Tokushima University Institutional Repository: 109786)
11.	Yoichiro Miyake, 板野守秀 and 矢納義高 : 糖アルコールとデンタルプラークコントロール, Journal of Medical Technology, Vol.52, No.4, 455-458, Apr. 2008. (Keyword) デンタルプラーク / 糖アルコール / エリスリトール (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1521980705067069568 (CiNii: 1521980705067069568)
12.	Yoichiro Miyake and Shizuo Kayama : Defence mechanisms to oral infectionq, Nihon Rinsho. Japanese Journal of Clinical Medicine, Vol.65, No.2, Feb. 2007. (Keyword) oral mucosa / 感染防御機構 / 唾液 / 歯肉溝 / 口腔常在菌
13.	Yoichiro Miyake : 基礎研究から臨床応用へ, Bacterial Adherence & Biofilm, Vol.19, 84-85, Feb. 2006.
14.	Yoichiro Miyake and Ikuko Hosokawa : β-defensin と口腔内感染, 臨床と微生物, Vol.32, No.3, 269-273, May 2005.
15.	Yoichiro Miyake and Ikuko Hosokawa : β-defensin と口腔内感染, 臨床と微生物, Vol.32, No.3, 269-273, May 2005.
16.	Yoichiro Miyake : 1．口腔ケアと細菌付着, Geriatric Medicine, Vol.43, No.11, 1745-1749, 2005. (Keyword) 口腔ケア / 口腔細菌 / 細菌付着 / biofilm / 高齢者
17.	Katsuhiko Hirota and Yoichiro Miyake : 知っておきたいう蝕原因菌と歯周病関連細菌, The Nippon Dental Review, Vol.64, No.2, 59-69, Feb. 2004.
18.	Yoichiro Miyake : 口腔の病原微生物と感染症, Geriatric Medicine, Vol.42, No.3, 287-291, 2004. (Keyword) oral care / 口腔細菌叢 / 誤嚥性肺炎 / 全身感染症 / 高齢者
19.	Yoichiro Miyake : 気道感染と口腔内細菌の役割, Dental Outlook, Vol.102, No.3, 629-633, Sep. 2003.
20.	Keiji Murakami and Yoichiro Miyake : 口腔細菌とQuorum sensing, Shikoku Dental Research, Vol.15, No.2, 251-256, Jan. 2003.
21.	Tsuneko Ono and Yoichiro Miyake : バイオフィルム形成菌における抗菌薬抵抗性のメカニズム, Shikoku Dental Research, Vol.14, No.2, 277-284, Jan. 2002. (Keyword) biofilm / 抗菌薬抵抗性 / 付着 / quorum sensing
22.	Yoichiro Miyake : Staphylococcus aureusの付着, 皮膚, Vol.40, No.20, 46-51, Dec. 1998.
23.	Yoichiro Miyake : カンジダ属，Staphylococcus aureus付着の解析および抗菌薬抵抗性における役割, Shikoku Dental Research, Vol.8, No.2, 127-139, Jan. 1996.
24.	Yoichiro Miyake : Bacterial Adherenceを考える, 侵襲と免疫, Vol.4, No.1, 10-16, Jan. 1995.

Proceeding of International Conference:

1.	Natsumi Fujiwara, Hiromichi Yumoto, Keiji Murakami, Yasusei Kudo, Hiromi Nakae, Katsuhiko Hirota, Kazumi Ozaki and Yoichiro Miyake : 2-methacryloyloxyethyl-phosphorylcholine (MPC)-polymer suppresses the adherence of oral bacteria, 2018 IADR/PER General Session (ExCel London Convention Center, London, England) 25-28th July, 2018, Jul. 2018.
2.	Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa, Hitomi Kuramoto, Katsuhiko Hirota, Yoichiro Miyake and Takashi Matsuo : Streptococcal histone-like DNA binding protein induces MINCLE expression on monocytes, 95th General Session & Exhibition of the International Association for Dental Research, San Francisco, Mar. 2017.
3.	Susilowati Heni, Keiji Murakami, Takashi Amoh, Katsuhiko Hirota, Yoichiro Miyake, Hiromichi Yumoto and Takashi Matsuo : Royal Jelly inhibits Pseudomonas aeruginosa adherence and reduces excessive inflammatory resoponses in human epithelial cells, 2016 IADR, Seoul, Jun. 2016.
4.	Yoichiro Miyake : Antibacterial tolerance of adheerent bacteria, Central Europeans Symposium on Antimicrobials and Antimicrobial Resistance, Croatia, Sep. 2015.
5.	Yoichiro Miyake : Aspiration Pneumonia in elderly and its prevention, 2nd Dental Research Exhibition And Meeting, Yogyakarta, Sep. 2014.
6.	Hiromichi Yumoto, Takashi Matsuo, Katsuhiko Hirota, Kouji Hirao, Miyazaki Tsuyoshi, Keiji Murakami, Natsumi Fujiwara and Yoichiro Miyake : Anti-inflammatory and protective effects of 2-methacryloyloxyethyl-phosphorylcholine-polymer on oral epithelial cells, 92th General Session & Exhibition of the International Association for Dental Research (Cape Town, South Africa), Jun. 2014.
7.	Nur Ashikin, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, 天羽崇 and Yoichiro Miyake : Extracellular DNA affects Streptococcus mutans biofilm through quorum sensing system and histone-like DNA binding protein expression, The 12th Japan-Korea International Symposium on Microbiology, Tokyo, Mar. 2014.
8.	Hiromichi Yumoto, Katsuhiko Hirota, Kouji Hirao, Keiji Murakami, Yoichiro Miyake and Takashi Matsuo : Pathogenic roles of Streptococcal histone-like protein in microbial infection, The 15th Joint Scientific Meeting of JSCD-KACD (Gyeongju, Korea), Nov. 2013.
9.	Momoyo Azuma, Keiji Murakami, Katuhiko Hirota, Tsuneko Ono, Yoichiro Miyake and Yasuhiko Nishioka : The relationship between antibiotic tolerance and severity of clinical isolated Pseudomonas aeruginosa in respiratory disease patients, The28th International Congress of Chemotherapy and Infection and the 14th Asia-pacific Society of Clinical Microbiology and Infection, 横浜, Jun. 2013.
10.	Keiji Murakami, Momoyo Azuma, Katsuhiko Hirota, Tsuneko Ono, Nishida Yasuhiko and Yoichiro Miyake : The correlation between antibiotic tolerance and virulence of Pseudomonas aeruginosa clinical isolates, 28th International Congress of Chemoteherap and Infection, Yokohama, Jun. 2013.
11.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake : Biological Roles of Extracellular DNA in Bacterial Infection, Asean Plus and Tokushima Joint International Conference, Yogyakarta, Dec. 2012.
12.	Kouji Hirao, Hiromichi Yumoto, Katsuhiko Hirota, Dali Liu, Nur Asikin, Kanako Takahashi, Takashi Matsuo and Yoichiro Miyake : Global Gene Analysis in Monocytes Stimulated with Streptococcal Histone-Like Protein, 90th General Session and Exhibition of the IADR, Iguazu Falls, Brazil, Jun. 2012.
13.	Liu Dali, Li Yunpeng, Zhou Yanbin, Naito Mariko, Hiromichi Yumoto, Yoichiro Miyake, Liang Jingping and Shu Rong : Genome Comparison of A Clinical Virulent Porphyromonas gingivalis Strain With Type Strains W83 And ATCC33277, International Union of Microbiological Societies 2011 Congress, Sep. 2011.
14.	Keiji Murakami, Darija Viducic, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : Pseudomonas aeruginosa psl genes play an important role in biapenem tolerance, International Union of Microbiological Societies 2011 Congress, Sapporo, Sep. 2011.
15.	Darija Viducic, Keiji Murakami, Tsuneko Ono and Yoichiro Miyake : The Pseudomonas aeruginosa quinolone signal plays a dual role in the response to antimicrobial agents, International Union of Microbiological Societies 2011 Congress, Sapporo, Sep. 2011.
16.	Katsuhiko Hirota, Hiromichi Yumoto, Nur Asikin, Kouji Hirao, Kanako Takahashi, Liu Dali, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake : Gene Expression Profiling of Human Monocytic THP-1 Cells Stimulated With Extracellular Streptococcus intermedius Histone-Like DNA Binding Protein, International Union of Microbiological Societies 2011 Congress, Sep. 2011.
17.	Keiji Murakami, Darija Viducic, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : The involvement of psl genes and the second messenger c-di-GMP in antibiotic tolerance of adherent Pseudomonas aeruginosa, EUROBIOFILMS 2011 -Second European Congress on Microbial Biofilms-Basic and Dlinical Aspedts-, Copenhagen, Jul. 2011.
18.	Katsuhiko Hirota, M Hori, S Hayakawa, Keiji Murakami and Yoichiro Miyake : Development of New Materials That Refuse Microbial Adherence, EUROBIOFILMS 2011 -Second European Congress on Microbial Biofilms-Basic and Dlinical Aspedts-, Copenhagen, Jul. 2011.
19.	Yoichiro Miyake, Katsuhiko Hirota, Hiromichi Yumoto, N Yamamoto, K Miyamoto and Takashi Matsuo : Inhibition of the adherence of oral bacterial by MPC-polymer, EUROBIOFILMS 2011 -Second European Congress on Microbial Biofilms-Basic and Dlinical Aspedts-, Copenhagen, Jul. 2011.
20.	Yoichiro Miyake : Antibiotic tolerance of Pseudomonas aeruginosa, a human commensal, International Symposium 62nd Anniversary of Gadjah Mada University School of Dentistry, Yogyakarta, Feb. 2010.
21.	Susilowati Heni, Hirohiko Okamura, Katsuhiko Hirota, Kaya Yoshida, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : Nuclear Translocation of NF-kB induced by Streptococcus intermedius intermedilysin, International Conference of Indonesian Society for Microbiology: Recent advances of Microbiology in Health, Agriculture, Bioindustry, Surabayai, Nov. 2009.
22.	Liu Dali, Hiromichi Yumoto, Liang Ping Jing, Yoichiro Miyake and Shu Rong : Recombinant Porphyromonas gingivalis DnaK purification and Pg-dnaK mutant creation, 2nd Meeting of IADR Pan Asian Pacific Federation and the 1st Meeting of IADR Asia/Pacific Region, Sep. 2009.
23.	Susilowati Heni, Hirohiko Okamura, Katsuhiko Hirota, Kaya Yoshida, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : The molecular mechanism for Streptococcus intermedius intermedilysin-induced cell death inhuman cholangiocellular carcinoma cells, International Joint Symposium Frontier in Biomedical Sciences: From Genes to Applications, Yogyakarta, Nov. 2008.
24.	Hiromichi Yumoto, Liu Dali, Keiji Murakami, Katsuhiko Hirota, Hideaki Nagamune, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake : Gene-regulatory and Physiological Functions of Histone-like Protein from Streptococci, 86th General Session and Exhibition of the International Association for Dental Research, Toronto (Canada), Jul. 2008.
25.	Susilowati Heni, Hirohiko Okamura, Katsuhiko Hirota, Kaya Yoshida, Keiji Murakami, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : NFAT1 activation in intermedilysin-induced human cholangiocellular carcinoma cell HuCCT1, The 2nd International Symposium on "The Future Direction of Oral Sciences in the 21st Century", Tokushima, Dec. 2007.
26.	Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake : The essentiality and involvements of streptocuccus intermedius histone-like protein in bacterial viability and normal growth, The 2nd International Symposium on "The Future Direction of Oral Sciences in the 21st Century", Tokushima, Dec. 2007.
27.	Heni Susilowati, Katsuhiko Hirota, Hirohiko Okamura, Kaya Yoshida, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : Intermedilysin induces cell death in HepG2 cells, 21st International Association for Dental Research - South East Asia Division, Bali, Indonesia, Sep. 2007.
28.	Shizuo Kayama, Keiji Murakami, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : Role of quorum sensings system of adherent pseudomonas aeruginosa in antibiotic tolerance, ASM conferences Pseudomonas 2007, Seattle, Aug. 2007.
29.	Liu Dali, Keiji Murakami, Hiromichi Yumoto, Katsuhiko Hirota and Yoichiro Miyake : Investigation of the toxicity of Streptococcus intermedius histone-like protein in Escherichia coli, The 1st international symposium and workshop ''The Future Direction of Oral Sciences in the 21st century'', Awajishima, Mar. 2007.
30.	Heni Susilowati, Katsuhiko Hirota, Hirohiko Okamura, Kaya Yoshida, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : The mechanisms of Intermedilysin to induce cell death on HepG2 and HuCCT1 cell lines, The 1st international symposium and workshop ''The Future Direction of Oral Sciences in the 21st century'', Awajishima, Mar. 2007.
31.	LIU Dali, Katsuhiko Hirota, Keiji Murakami and Yoichiro Miyake : pathogenicity of streptococcus intermedius,an Oral Commensal Bacterium, 2005-SINO-JAPANESE CONFERENCE ON STOMATOLOGY, Shanghai, Nov. 2005.
32.	Hideaki Nagamune, Akiko Sukeno, Robert A. Whiley, Syed I. Jafar, Joseph Aduse-Opoke, Kazuto Ohkura, Takuya Maeda, Katsuhiko Hirota, Yoichiro Miyake and Hiroki Kourai : Intermedilysin plays a key role in the invasion of human cells by Streptococcus intermedius, XVIth Lancefield International Symposium on Streptococci and Streptococcal Diseases: In "Streptococci-New insights into an old enemy", International Congress Series, Vol.1289, 262-266, Palm cove, Australia, Sep. 2005.
33.	Keiji Murakami, Kayama Sizuo, Viducic Darija, Tsuneko Ono and Yoichiro Miyake : The involvement of pslL gene in biapenem tolerance of Pseudomonas aeruginosa, Pseudomonas 2005 10th International Congress Abstract Book, 139, Marseille, Aug. 2005.
34.	Darija Viducic, Tsuneko Ono, Keiji Murakami and Yoichiro Miyake : A role of ppGpp in tolerance to quinolone in Pseudomonas aeruginosa, Pseudomonas 10th International congress Abstract Book, 101, Marseille, Aug. 2005.
35.	Hiromichi Yumoto, Katsuhiko Hirota, Hideaki Nagamune, Kazuto Ohkura, Keiji Murakami, Masayoshi Yamada, Hideaki Nakae, Yoichiro Miyake and Takashi Matsuo : Molecular analysis of histone-like protein from Streptococcus intermedius, 80th General Session and Exhibition of the International Association for Dental Research, San Diego(USA), Mar. 2002.
36.	Naoki Ohmura, Katsuhiko Hirota, Hiromichi Yumoto, Keiji Murakami, Hideo Kanitani, Toshiya Kashiwabara, Yoichiro Miyake and Tetsuo Ichikawa : Influence of denture plaque on the pharyngeal microflora, 80th General Session and Exhibition of the International Association for Dental Research, San Diego(USA), Mar. 2002.
37.	Kazuto Ohkura, Takatsugu Goto, Kanako Hattori, Ohki Ohnishi, Hideaki Tsuge, Nobuhiko Katunuma, Katsuhiko Hirota, Yoichiro Miyake, Hiroki Kourai and Hideaki Nagamune : Structural Analysis of a Human Specific Cytolysin Intermedilysin from Streptococcus intermedius UNS46, 18th International Congress of Biochemistry and Molecular Biology, Birmingham, UK, Jul. 2000.
38.	Hideaki Nagamune, Ohki Ohnishi, Kanako Hattori, Kazuto Ohkura, Takatsugu Goto, Hideaki Tsuge, Katsuhiko Hirota, Nobuhiko Katunuma, Yoichiro Miyake, Takuya Maeda and Hiroki Kourai : Human-Specific Cell Lysis by Intermedilysin, an Exotoxin of Streptococcus intermedius, 18th International Congress of Biochemistry and Molecular Biology, Birminghan, UK, Jul. 2000.

Proceeding of Domestic Conference:

1.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota, 公文裕巳, Yoichiro Miyake and Hideki Fujii : 浮遊菌とバイオフィルム形成菌の抗菌薬抵抗性に対するrpoS遺伝子の役割, 第32回日本バイオフィルム学会学術集会, Jul. 2018.
2.	Keiji Murakami, Takashi Amoh, Keiko Kataoka, 狩山玲子, Katsuhiko Hirota, Yoichiro Miyake and Hideki Fujii : 緑膿菌オートインデューサーアナログのマクロライドに対する効果, 第91回日本細菌学会総会, Mar. 2018.
3.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota, Yoichiro Miyake and Hideki Fujii : 緑膿菌rpoS遺伝子が抗菌薬抵抗性に及ぼす役割, 第91回日本細菌学会総会, Mar. 2018.
4.	Keiji Murakami, Kariyama Reiko, Kumon Hiromi and Yoichiro Miyake : 緑膿菌Autoinducer Analogがマクロライドの抗菌作用に及ぼす影響について, 第52回緑膿菌感染症研究会, Feb. 2018.
5.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota, Yoichiro Miyake and Hideki Fujii : rpoS遺伝子が緑膿菌の抗菌薬抵抗性に及ぼす影響, 第70回日本細菌学会中国・四国支部総会, Oct. 2017.
6.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota, 公文裕巳 and Yoichiro Miyake : 緑膿菌の抗菌薬抵抗性に対する新規化合物の作用機序, 第31回日本バイオフィルム学会学術集会, Jul. 2017.
7.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌における新規化合物の抗菌薬抵抗性に対する作用機序の解析, 第90回日本細菌学会総会, Mar. 2017.
8.	Keiji Murakami, Takashi Amoh, Katsuhiko Hirota, Keiko Kataoka and Yoichiro Miyake : 緑膿菌呼吸器感染症における抗菌薬抵抗性の臨床的重要性について「シンポジウム不均一性細菌学:表現型の不均一性に基づいた細菌の生存戦略」, 第90回日本細菌学会総会, Mar. 2017.
9.	Keiji Murakami, 狩山玲子, 公文裕巳 and Yoichiro Miyake : 緑膿菌の抗菌薬抵抗性に及ぼすAutoinducer Analogの作用機序について, 第51回緑膿菌感染症研究会, Feb. 2017.
10.	Shoto Ogawa, Shinichi Fujie, Keiji Murakami, Takashi Amoh, Yoichiro Miyake, 佐藤雅美, 笹田倫子, 菅崎幹樹, 香川葉子, Yuri Morimoto, Momoyo Azuma, Akiko Sakurai and Keiko Kataoka : 緑膿菌オートインデューサー類似化合物が抗菌薬抵抗性に及ぼす影響, 第40回徳島県医学検査学会, Dec. 2016.
11.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota and Yoichiro Miyake : 新規化合物の作用機序およびバイオフィルムに対する効果, 第69回日本細菌学会中国・四国支部総会, 38, Oct. 2016.
12.	Natsumi Fujiwara, Keiji Murakami, Hiromichi Yumoto, Katsuhiko Hirota and Yoichiro Miyake : 歯周病原菌に対する新規化合物の効果, 第69回日本細菌学会中国・四国支部総会平成28年10月15-16日香川県高松市かがわ国際会議場, 38, Oct. 2016.
13.	Natsumi Fujiwara, Keiji Murakami, Hiromichi Yumoto, Katsuhiko Hirota, Kazumi Ozaki and Yoichiro Miyake : Effects of novel compounds on periodontopathic bacteria, 第58回歯科基礎医学会学術大会北海道札幌市 2016年8月24-26日, Aug. 2016.
14.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota, 公文裕巳 and Yoichiro Miyake : 新規化合物によるレボフロキサシン及びトブラマイシン殺菌作用の増強効果, 第30回バイオフィルム学会学術集会, Jul. 2016.
15.	Kazuto Shiraishi, Keiji Murakami, Noriko Saito-Tarashima, Kazuhiro Furukawa, Yoichiro Miyake and Noriaki Minakawa : ホスホジエステラーゼ抵抗性を有する4'-チオ化学修飾型環状ジヌクレオチドの合成と評価, 日本薬学会第136年会, Mar. 2016.
16.	Katsuhiko Hirota, Hiromichi Yumoto, Keiji Murakami, Kouji Hirao, Takashi Amoh and Yoichiro Miyake : 口腔常在菌Streptococcus intermediusが呼吸器疾患重症化に関与するメカニズム, 第89回日本細菌学会総会, Mar. 2016.
17.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota and Yoichiro Miyake : 新規化合物が耐性菌やバイオフィルムに及ぼす影響, 第89回日本細菌学会総会, Mar. 2016.
18.	Keiji Murakami, Takashi Amoh, Katsuhiko Hirota and Yoichiro Miyake : 付着菌およびバイオフィルム形成菌の抗菌薬抵抗性とそのメカニズム, 第89回日本細菌学会総会, Mar. 2016.
19.	Keiji Murakami, Takashi Amoh, Katsuhiko Hirota and Yoichiro Miyake : クラリスロマイシンによる緑膿菌c-di-GMPの誘導, 第50回緑膿菌感染症研究会, Feb. 2016.
20.	Ayumi Yoshida, Mai Ueta, Keiji Murakami, Yoichiro Miyake, 佐藤雅美, 笹田倫子, 菅崎幹樹, 香川葉子, Yuri Morimoto, Momoyo Azuma, Akiko Sakurai and Keiko Kataoka : 緑膿菌におけるストレス応答と抗菌薬抵抗性について, 第39回徳島県医学検査学会, Dec. 2015.
21.	Mai Ueta, Ayumi Yoshida, Keiji Murakami, Yoichiro Miyake, 佐藤雅美, 笹田倫子, 菅崎幹樹, 香川葉子, Yuri Morimoto, Momoyo Azuma, Akiko Sakurai and Keiko Kataoka : リボザイムを応用したcyclic-di-GMPの定量法の開発, 第39回徳島県医学検査学会, Dec. 2015.
22.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota, 公文裕巳 and Yoichiro Miyake : 緑膿菌AI類似化合物は緑膿菌に対するビアペネムの殺菌効果を増強する, 第63回日本化学療法学会西日本支部総会, Oct. 2015.
23.	Keiji Murakami, 狩山玲子, Takashi Amoh, Katsuhiko Hirota, 公文裕巳 and Yoichiro Miyake : 緑膿菌psl遺伝子が抗菌薬抵抗に及ぼす影響, 第63回日本化学療法学会西日本支部総会, Oct. 2015.
24.	Takashi Amoh, Keiji Murakami, 狩山玲子, Katsuhiko Hirota and Yoichiro Miyake : 新規化合物による抗菌薬殺菌作用の増強効果について, 第68回日本細菌学会中国・四国支部総会, Oct. 2015.
25.	Keiji Murakami, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌AHL類似化合物によるビアペネム殺菌効果の増強について, 第57回歯科基礎医学会学術大会, Sep. 2015.
26.	Liu Dali, Zhou Yanbin, Li Yunpeng, Hiromichi Yumoto, Yoichiro Miyake, Liang Jingping and Shu Rong : Pan-genome and comparative genome of 16 Porphyromonas gingivalis strains, 第58回秋季日本歯周病学会学術大会, Sep. 2015.
27.	Katsuhiko Hirota, Hiromichi Yumoto, Keiji Murakami, Kouji Hirao, Takashi Amoh and Yoichiro Miyake : Streptococcus intermedius biofilm componentsの糖鎖結合能と炎症反応誘導に関する解析, 第29回日本バイオフィルム学術集会, Jul. 2015.
28.	天羽崇, Keiji Murakami, 狩山玲子, Katsuhiko Hirota, 公文裕巳 and Yoichiro Miyake : 新規化合物によるビアペネム殺菌作用の増強効果について, 第29回日本バイオフィルム学会学術集会, Jul. 2015.
29.	白石和人, Keiji Murakami, Noriko Saito-Tarashima, Kazuhiro Furukawa, Yoichiro Miyake and Noriaki Minakawa : 糖部修飾型環状ジヌクレオチドの合成と評価, 創薬懇話会2015 in 徳島, Jul. 2015.
30.	白石和人, Keiji Murakami, Noriko Saito-Tarashima, Kazuhiro Furukawa, Yoichiro Miyake and Noriaki Minakawa : Cyclic-di-4'-S-adenosine monophosphate (c-di-SAMP)の合成と評価, 第17回プロセス化学東四国フォーラムセミナー, Jun. 2015.
31.	Keiji Murakami, 狩山玲子, 堀賢司, 光畑律子, Katsuhiko Hirota, 公文裕巳 and Yoichiro Miyake : 新規化合物が薬剤耐性緑膿菌に対する殺菌効果に及ぼす影響, 第63回日本化学療法学会総会, Jun. 2015.
32.	天羽崇, Keiji Murakami, 狩山玲子, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌の抗菌薬抵抗性に対する新規化合物の効果, 第88回日本細菌学会総会, Mar. 2015.
33.	Katsuhiko Hirota, Hiromichi Yumoto, Keiji Murakami, Kouji Hirao, 天羽崇 and Yoichiro Miyake : Streptococcus intermedius Histone-Like Proteinと細胞外ベシクルの特性, 第88回日本細菌学会総会, Mar. 2015.
34.	Keiji Murakami, Hideaki Maseda, 天羽崇, Katsuhiko Hirota, Keiko Kataoka and Yoichiro Miyake : 臨床および環境分離緑膿菌の抗菌薬抵抗性と病原因子に関する検討, 第88回日本細菌学会総会, Mar. 2015.
35.	Yukari Yoshikawa, Shoko Kannuki, Keiji Murakami, Yoichiro Miyake, 畑美智子, 佐藤雅美, 笹田倫子, 香川葉子, 菅崎幹樹, Momoyo Azuma, Akiko Sakurai and Keiko Kataoka : 本院で分離されたST-8型MRSAについて, 第38回徳島県医学検査学会, Dec. 2014.
36.	Shoko Kannuki, Yukari Yoshikawa, Keiji Murakami, Yoichiro Miyake, Hideaki Maseda, 畑美智子, 佐藤雅美, 笹田倫子, 香川葉子, 菅崎幹樹, Momoyo Azuma, Akiko Sakurai and Keiko Kataoka : 臨床および環境分離緑膿菌における病原性について, 第38回徳島県医学検査学会, Dec. 2014.
37.	狩山玲子, 堀賢司, Keiji Murakami, 光畑律子, 和田耕一郎, 渡辺豊彦, Yoichiro Miyake and 公文裕巳 : OprD欠損緑膿菌によるマウス大腿部感染モデルに対するクオラムセンシング阻害剤とビアペネムの併用効果, 第62回日本化学療法学会西日本支部総会第57回日本感染症学会中日本地方会学術集会第84回日本感染症学会西日本地方会学術集会合同開催, Oct. 2014.
38.	Keiji Murakami, 狩山玲子, 堀賢司, 光畑律子, Katsuhiko Hirota, 公文裕巳 and Yoichiro Miyake : 緑膿菌におけるクオラムセンシング阻害剤が抗菌薬抵抗性に及ぼす影響, 第62回日本化学療法学会西日本支部総会第57回日本感染症学会中日本地方会学術集会第84回日本感染症学会西日本地方会学術集会合同開催, Oct. 2014.
39.	Katsuhiko Hirota, Hiromichi Yumoto, Keiji Murakami, Kouji Hirao, 天羽崇, Takashi Matsuo and Yoichiro Miyake : Streptococcus intermedius Histone-Like DNA Binding Proteinの結合解析とExtracellular Vesicleとの関連, 第67回日本細菌学会中国・四国支部総会, Oct. 2014.
40.	天羽崇, Keiji Murakami, 狩山玲子, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌の抗菌薬抵抗性に対する新規化合物の効果, 第67回日本細菌学会中国・四国支部総会, Oct. 2014.
41.	Keiji Murakami, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌臨床分離株における抗菌薬抵抗性と病原性との関連, 第56回歯科基礎医学会学術大会, Sep. 2014.
42.	Omar Maningo Rodis, Yoichiro Miyake, Yoshizo Matsuka and Bukasa Kalubi : Globalization Efforts of the University of Tokushima Faculty of Dentistry, 17th JASMEE Academic Meeting, Jul. 2014.
43.	Keiji Murakami, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌の抗菌薬抵抗性と病原因子に関する検討, 第88回日本感染症学会学術講演会第62回日本化学療法学会総会合同学会, Jun. 2014.
44.	堀賢司, 狩山玲子, Keiji Murakami, 光畑律子, 和田耕一郎, 石川亜矢乃, 渡辺豊彦, Yoichiro Miyake and 公文裕巳 : 新規マウス薬効評価系の構築に向けた発光性緑膿菌による尿路バイオフィルム感染症モデルの作製, 第88回日本感染症学会学術講演会第62回日本化学療法学会総会合同学会, Jun. 2014.
45.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Keiji Murakami and Yoichiro Miyake : Extracellular DNA influences pyocyanin production from Pseudomonas aeruginosa, 第87回日本細菌学会総会, Mar. 2014.
46.	Katsuhiko Hirota, Hiromichi Yumoto, Keiji Murakami, Kouji Hirao and Yoichiro Miyake : 抗Si-HLP抗体のミトコンドリアATP synthase β subunitに対する交差反応性, 第87回日本細菌学会総会, Mar. 2014.
47.	Keiji Murakami, Nur Asikin, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌臨床分離株における抗菌薬抵抗性と病原性との関連, 第87回日本細菌学会総会, Mar. 2014.
48.	Tomoko Kimura, Kazumi Ozaki, Hiromichi Yumoto, Keiji Murakami, Chieko Sugawara, Chihiro Shinohara, Emi Takegawa, Yoichiro Miyake, Takashi Matsuo and Fumiaki Kawano : Roles of scrA gene in pathogenicity of Streptococcus mutans, 第6回日本総合歯科学会総会・学術大会, 38, Nov. 2013.
49.	Hideaki Maseda, 上手麻希, Keiji Murakami and Yoichiro Miyake : 環境および臨床由来緑膿菌の特性比較, 第61回日本化学療法学会西日本支部総会, Nov. 2013.
50.	堀賢司, 狩山玲子, 光畑律子, Keiji Murakami, 和田耕一郎, 石井亜矢乃, 渡辺豊彦, Yoichiro Miyake and 公文裕巳 : マウスを用いた緑膿菌性尿路バイオフィルム感染症モデルの作製と各種抗菌薬の治療効果, 第61回日本化学療法学会西日本支部総会, Nov. 2013.
51.	Keiji Murakami, Tsuneko Ono, Natsumi Takamaru, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌臨床分離株による抗菌薬抵抗性と病原因子との関連について, 第61回日本化学療法学会西日本支部総会, Nov. 2013.
52.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami and Yoichiro Miyake : Homologous and heterologous extracellular DNAs affect Pseudomonas aeruginosa to produce more pyocyanin without inducing bacterial growth, 第66回日本細菌学会中国・四国支部総会, Oct. 2013.
53.	Keiji Murakami, Yumi Hoshimo, Katsuhiko Hirota and Yoichiro Miyake : イグサ抽出液によるう蝕および歯周病予防効果の検討, 第55回歯科基礎医学会学術大会, Sep. 2013.
54.	Keiji Murakami, Tsuneko Ono, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌における抗菌薬抵抗性とpsl遺伝子の役割, 第7回細菌学若手コロッセウム, Aug. 2013. (Keyword) Pseudomonas aeruginosa / 抗菌薬抵抗性 / psl遺伝子
55.	狩山玲子, 堀賢司, 光畑律子, Keiji Murakami, 和田耕一郎, 石井亜矢乃, 渡辺豊彦, Yoichiro Miyake and 公文裕巳 : マウスを用いた緑膿菌性尿路バイオフィルム感染症モデルの作製, 第27回Bacterial Adherence & Biofilm学術集会, Jul. 2013.
56.	Keiji Murakami, Nur Asikin, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌臨床分離株における抗菌薬抵抗性と病原因子との関連について, 第27回Bacterial Agherence & Biofilm学術集会, Jul. 2013.
57.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake : Effect of extracellular DNA on pyocyanin production from Pseudomonas aeruginosa, 第27回Bacterial Agherence & Biofilm学術集会, Jul. 2013.
58.	Katsuhiko Hirota, Hiromichi Yumoto, Keiji Murakami, Kouji Hirao, Kanako Takahashi, Takashi Matsuo and Yoichiro Miyake : Streptococcus intermediusヒストン様タンパク質のPAMPsとしての解析, 第22回Lancefieldレンサ球菌研究会&第45回レンサ球菌感染症研究会, Jun. 2013.
59.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami and Yoichiro Miyake : The role of extracellular DNA in Pseudomonas aeruginosa and Streptococcus intermedius infection, 第86回日本細菌学会総会, Mar. 2013.
60.	Keiji Murakami, Tsuneko Ono, Katsuhiko Hirota and Yoichiro Miyake : Effects of theaddition of Clarithromycin on Pseudomonas aeruginosa biofilm formation, 第86回日本細菌学会総会, Mar. 2013.
61.	Katsuhiko Hirota, Hiromichi Yumoto, Nur Asikin, Kouji Hirao, Keiji Murakami and Yoichiro Miyake : Pathogenic roles of Streptococcus intermedius histone-like protein in microbial inflammation, 第86回日本細菌学会総会, Mar. 2013.
62.	白水裕也, 星野佑太, 西田みなみ, 曽宮葉子, Chieko Matsuura, Tsuneko Ono, Keiji Murakami, Yoichiro Miyake, Akiko Sakurai, 佐藤雅美, 岡本貴子, 香川葉子 and 畑美智子 : 緑膿菌臨床分離株における抗菌薬耐性のメカニズム, 第36回徳島県医学検査学会, Dec. 2012.
63.	星野佑太, 白水裕也, 西田みなみ, 曽宮葉子, Chieko Matsuura, Tsuneko Ono, Keiji Murakami, Yoichiro Miyake, Akiko Sakurai, 佐藤雅美, 岡本貴子, 香川葉子 and 畑美智子 : 緑膿菌臨床分離株における抗菌薬抵抗性と病原因子について, 第36回徳島県医学検査学会, Dec. 2012.
64.	Keiji Murakami, Tsuneko Ono, Natsumi Takamaru, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌における付着及びバイオフィルム形成菌の抗菌薬抵抗性遺伝子のスクリーニング, 第60回日本化学療法学会西日本支部総会, Nov. 2012.
65.	江頭陽子, Keiji Murakami, Katsuhiko Hirota, Hiroki Iga and Yoichiro Miyake : 新規抗菌性界面活性剤の臨床応用を目指した基礎研究, 第65回日本細菌学会中国・四国支部総会, Oct. 2012.
66.	西田みなみ, 曽宮葉子, Tsuneko Ono, Keiji Murakami, Katsuhiko Hirota and Yoichiro Miyake : クラリスロマイシンによる緑膿菌RSCVsの誘導について, 第65回日本細菌学会中国・四国支部総会, Oct. 2012.
67.	曽宮葉子, 西田みなみ, Keiji Murakami, Tsuneko Ono and Yoichiro Miyake : 緑膿菌の抗菌薬抵抗性に及ぼすpsl遺伝子とc-di-GMPの関与について, 第65回日本細菌学会中国・四国支部総会, Oct. 2012.
68.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami and Yoichiro Miyake : Biological roles of extracellular DNA of Streptococcus intermeius, 第65回日本細菌学会中国・四国支部総会, Oct. 2012.
69.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake : Role of Extracellular DNA and DNA-binding protein in biofilm formation of Streptococcus intermeius, 第26回Bacterial Adherence & Biofilm学術集会, Jul. 2012.
70.	Keiji Murakami, Tsuneko Ono, Katsuhiko Hirota and Yoichiro Miyake : 付着及びバイオフィルム形成緑膿菌の抗菌薬抵抗性関連遺伝子の探索, 第26回Bacterial Adherence & Biofilm学術集会, Jul. 2012.
71.	Yoichiro Miyake : シンポジウム「再考!バイオフィルム」口腔細菌のトランスロケーションによる感染症とその予防 -誤嚥性肺炎を中心に-, 第26回Bacterial Adherence & Biofilm学術集会, Jul. 2012.
72.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake : Roles of extracellular DNA and DNA-binding protein in cell aggregation, biofilm formation and antibiotic tolerance of Streptococcus intermedius, 第21回Lancefieldレンサ球菌研究会&第44回レンサ球菌感染症研究会合同開催, Jun. 2012.
73.	Keiji Murakami, Tsuneko Ono, Katsuhiko Hirota and Yoichiro Miyake : クラリスロマイシン添加による緑膿菌への影響, 第60回日本化学療法学会学術集会, Apr. 2012.
74.	Yohko Egashira, Keiji Murakami, Katsuhiko Hirota, Hiroki Iga and Yoichiro Miyake : 新規抗菌性界面活性剤の臨床応用を目指した基礎研究, 四国歯学会第40回例会, Mar. 2012.
75.	Yumi Hoshimo, Keiji Murakami, Katsuhiko Hirota, Daisuke Hinode and Yoichiro Miyake : イグサ粉末の口腔細菌に対する効果, 四国歯学会第40回例会, Mar. 2012.
76.	Yoichiro Miyake : 口腔細菌の病原性とそのコントロール, POIC研究開設率記念講演全身と口腔感染症予防の革新的幕開け∼新しい医療連携を目指して∼, Mar. 2012.
77.	Maki Yamada, Keita Maki, Naoko Okasaka, Chieko Matsuura, Tsuneko Ono, Keiji Murakami, Katsuhiko Hirota, Yoichiro Miyake, Issei Minase, Yasuki Noma, Yohko Somiya and Minami Nishida : 緑膿菌臨床分離株におけるpel, psl遺伝子の保有と抗菌薬抵抗性との関連について, 第35回徳島県医学検査学会, Dec. 2011.
78.	Keita Maki, Naoko Okasaka, Maki Yamada, Chieko Matsuura, Tsuneko Ono, Keiji Murakami, Katsuhiko Hirota, Yoichiro Miyake, Issei Minase, Yasuki Noma, Yohko Somiya and Minami Nishida : 緑膿菌臨床分離株におけるバイオフィルム形成能と薬剤感受性について, 第35回徳島県医学検査学会, Dec. 2011.
79.	Naoko Okasaka, Maki Yamada, Keita Maki, Chieko Matsuura, Tsuneko Ono, Keiji Murakami, Katsuhiko Hirota, Yoichiro Miyake, Issei Minase, Yasuki Noma, Yohko Somiya and Minami Nishida : 徳島大学病院で分離された緑膿菌における薬剤耐性のメカニズムとERIC-PCRの有用性について, 第35回徳島県医学検査学会, Dec. 2011.
80.	Keiji Murakami, Tsuneko Ono, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌における抗菌薬抵抗性関連遺伝子の探索, 第59回日本化学療法学会西日本支部総会, Nov. 2011.
81.	Minami Nishida, Yasuki Noma, Issei Minase, Yohko Somiya, Keiji Murakami, Yoichiro Miyake and Tsuneko Ono : クラリスロマイシンが及ぼす緑膿菌コロニー性状への影響, 第44回中国四国医学検査学会, Nov. 2011.
82.	Yohko Somiya, Yasuki Noma, Issei Minase, Minami Nishida, Keiji Murakami, Yoichiro Miyake and Tsuneko Ono : 緑膿菌psl遺伝子の抗菌薬抵抗性への関与について, 第44回中国四国医学検査学会, Nov. 2011.
83.	Issei Minase, Yasuki Noma, Yohko Somiya, Minami Nishida, Keiji Murakami, Yoichiro Miyake and Tsuneko Ono : バイオフィルム形成菌での抗菌薬抵抗性獲得メカニズムについて, 第44回中国四国医学検査学会, Nov. 2011.
84.	Yasuki Noma, Issei Minase, Yohko Somiya, Minami Nishida, Keiji Murakami, Yoichiro Miyake and Tsuneko Ono : 付着緑膿菌のビアペネム抵抗性関連遺伝子の探索, 第44回中国四国医学検査学会, Nov. 2011.
85.	Yasuki Noma, Keiji Murakami, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 付着緑膿菌における抗菌薬抵抗性に関与する遺伝子の検討, 第64回日本細菌学会中国・四国支部総会, Oct. 2011.
86.	Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami and Yoichiro Miyake : The Effect of Extracellular DNA on The Development of Streptococcus intermedius Biofilm, 第64回日本細菌学会中国・四国支部総会, Oct. 2011.
87.	Yoichiro Miyake : シンポジウムI「バイオフィルム見聞録」バイオフィルムの病原性とその対策, 日本歯科保存学会2011年度秋季学術大会(第135回), Oct. 2011.
88.	Keiji Murakami and Yoichiro Miyake : バイオフィルム形成緑膿菌における抗菌薬抵抗性関連遺伝子の探索, 第53回歯科基礎医学会学術大会, Sep. 2011.
89.	李景, Toshiya Kashiwabara, Yoshihito Naitou, Hideki Suito, Katsuhiko Hirota, Yoichiro Miyake and Tetsuo Ichikawa : バイオフィルムに対するUV-LEDパルス照射の消毒効果, LED総合フォーラム2011 in 徳島, Jun. 2011.
90.	李景, Toshiya Kashiwabara, Yoshihito Naitou, 水頭英樹, Katsuhiko Hirota, Yoichiro Miyake and Tetsuo Ichikawa : バイオフィルムに対するUV-LEDパルス照射の消毒効果, LED総合フォーラム2011 in 徳島, Jun. 2011.
91.	Kan Nagao, Natsumi Fujiwara, Tomoko Kimura, Akihiro Yasue, Masami Yoshioka, Yoichiro Miyake, Katsuhiko Yoshimoto and Tetsuo Ichikawa : 東京医科歯科大学の臨床実習視察報告, 四国歯学会第38回例会, Mar. 2011.
92.	Hisahiro Takeuchi, Toshiyuki Suge, Yukihiro Momota, Kazumi Ozaki, Atsuko Nakamichi, Yoichiro Miyake, Katsuhiko Yoshimoto and Tetsuo Ichikawa : 日本歯科大学臨床実習視察報告, 第38回四国歯学会例会, Mar. 2011.
93.	近藤洋史, Tsuneko Ono, Katsuhiko Hirota, Keiji Murakami, Yoichiro Miyake, Hiromichi Yumoto, Kanako Takahashi, Kouji Hirao and Takashi Matsuo : THP-1細胞のケモカイン産生に及ぼす口腔レンサ球菌ヒストン様DNA結合タンパク質の影響, 第34回徳島県医学検査学会, Dec. 2010.
94.	小寺佑佳, Yohko Somiya, 西田みなみ, Chieko Matsuura, Tsuneko Ono, Keiji Murakami, Katsuhiko Hirota, Yoichiro Miyake, Issei Minase and Yasuki Noma : 緑膿菌臨床分離株のRSCVの分離と抗菌薬感受性について, 第34回徳島県医学検査学会, Dec. 2010.
95.	西田みなみ, 小寺佑佳, Yohko Somiya, Chieko Matsuura, Tsuneko Ono, Keiji Murakami, Katsuhiko Hirota, Yoichiro Miyake, Issei Minase and Yasuki Noma : 抗菌薬による緑膿菌RSCVの誘導について, 第34回徳島県医学検査学会, Dec. 2010.
96.	Yohko Somiya, 小寺佑佳, 西田みなみ, Chieko Matsuura, Tsuneko Ono, Keiji Murakami, Shizuo Kayama, Katsuhiko Hirota, Yoichiro Miyake, Issei Minase and Yasuki Noma : 緑膿菌におけるc-di-GMPと抗菌薬抵抗性について, 第34回徳島県医学検査学会, Dec. 2010.
97.	Keiji Murakami, Shizuo Kayama, Tsuneko Ono, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌におけるc-di-GMPと抗菌薬抵抗性との関連について, 第58回日本化学療法学会西日本支部総会, Nov. 2010.
98.	Yumi Hoshimo, Keiji Murakami and Yoichiro Miyake : イグサの口腔細菌に及ぼす影響について, 第63回日本細菌学会中国・四国支部総会, Oct. 2010.
99.	Issei Minase, Shizuo Kayama, Keiji Murakami, Tsuneko Ono and Yoichiro Miyake : 緑膿菌ladS遺伝子の抗菌薬抵抗性への影響について, 第63回日本細菌学会中国・四国支部総会, Oct. 2010.
100.	Kouji Hirao, Hiromichi Yumoto, Katsuhiko Hirota, Kanako Takahashi, Tomoko Kimura and Yoichiro Miyake : Streptococcus intermedius由来histon様DNA結合タンパク質がヒト単球系細胞のサイトカイン・ケモカイン産生に及ぼす影響, 第63回日本細菌学会中国・四国支部総会, Oct. 2010.
101.	Katsuhiko Hirota and Yoichiro Miyake : Streptococcus intermediusヒストン様タンパク質がTHP-1細胞のサイトカインバランスに及ぼす影響, 第52回歯科基礎医学会学術大会, Sep. 2010.
102.	Katsuhiko Hirota, Hiromichi Yumoto, Yumi Hoshimo, Keiji Murakami, 宮本幸治, Takashi Matsuo and Yoichiro Miyake : 水溶性MPCポリマーの口腔細菌付着抑制効果, Bacterial Adherence &Biofilm学術集会, Jul. 2010.
103.	Keiji Murakami and Yoichiro Miyake : 緑膿菌臨床分離株におけるRSCVsの分離とその性状について, Bacterial Adherence &Biofilm学術集会, Jul. 2010.
104.	Yoichiro Miyake : 緑膿菌の抗菌薬抵抗性メカニズムの解明に向けて, Bacterial Adherence &Biofilm学術集会, Jul. 2010.
105.	Keiji Murakami, Shizuo Kayama, Tsuneko Ono and Yoichiro Miyake : 緑膿菌臨床分離株におけるRSCVの分離と抗菌薬抵抗性について, 第58回日本化学療法学会学術講演会, Jun. 2010.
106.	Shizuo Kayama, Keiji Murakami, Tsuneko Ono, 山本明毅, 谷口友伯, Katsuhiko Hirota, Hideaki Nagamune and Yoichiro Miyake : The role of QS Las-system and sigma factors in antibiotic tolerance in Pseudomonas aeruginosa, 第83回日本細菌学会総会, Mar. 2010.
107.	山本泰裕, Atsushi Tabata, Toshifumi Tomoyasu, Katsuhiko Hirota, Yoichiro Miyake, Hideaki Nagamune and 大國寿士 : Analysis of a novel S.mitis strain possessing two distinct cholesterol-dependent cytolysin genes, 第83回日本細菌学会総会, Mar. 2010.
108.	Tomoko Kimura, Kazumi Ozaki, Hiromichi Yumoto, Keiji Murakami, Shizuo Kayama, 藤坂菊美, Kanako Takahashi and Yoichiro Miyake : Roles and virulence of scrA and htrA genes in Streptococcus mutans, 第83回日本細菌学会総会, Mar. 2010.
109.	稲垣覚, Katsuhiko Hirota, 濱田了, 葛岡篤史, 石原和幸 and Yoichiro Miyake : Envaluation of measurement of oral bacteria by rapid microbial detection system using DEPM method, 第83回日本細菌学会総会, Mar. 2010.
110.	Keiji Murakami and Yoichiro Miyake : The mecanism of pel induction in tube biofilm, 第83回日本細菌学会総会, Mar. 2010.
111.	Katsuhiko Hirota, Shizuo Kayama, Keiji Murakami, Hiromichi Yumoto and Yoichiro Miyake : Molecular mechanisms of Intermedilysin induced human ER disfunction, 第83回日本細菌学会総会, Mar. 2010.
112.	Eijiro Sakamoto, Masami Ninomiya, Satoshi Yoneda, 徳永格, 中島由紀子, フルジャフジャフラン, Katsuhiko Hirota, Yoichiro Miyake, 竹田洋子, 武村あかね and Toshihiko Nagata : ハムスター実験的歯周炎におけるL-Cysteinの歯槽骨吸収抑制効果, 四国歯学会第36回例会, Mar. 2010.
113.	坂本英次郎, Masami Ninomiya, Satoshi Yoneda, 徳永格, 中島由紀子, フルジャフジャフラン, Katsuhiko Hirota, Yoichiro Miyake, 竹田洋子, 武村あかね and Toshihiko Nagata : ハムスター実験的歯周炎におけるL-Cysteinの歯槽骨吸収抑制効果, 四国歯学会第36回例会, Mar. 2010.
114.	高谷亜加里, 福田幸恵, Tsuneko Ono, Chieko Matsuura, Shizuo Kayama, Keiji Murakami, Katsuhiko Hirota, Yoichiro Miyake, 山本明毅 and 谷口友伯 : 緑膿菌臨床分離株におけるwsp関連遺伝子と抗菌薬抵抗性に関する研究, 第33回徳島県医学検査学会, Dec. 2009.
115.	福田幸恵, 高谷亜加里, Tsuneko Ono, Chieko Matsuura, Shizuo Kayama, Keiji Murakami, Katsuhiko Hirota, Yoichiro Miyake, 山本明毅 and 谷口友伯 : 緑膿菌におけるσ因子関連遺伝子と抗菌薬抵抗性に関する研究, 第33回徳島県医学検査学会, Dec. 2009.
116.	山本明毅, Tsuneko Ono, Shizuo Kayama, 谷口友伯, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌シグマ因子が抗菌薬抵抗性に及ぼす影響, 第57回日本化学療法学会西日本支部総会, Nov. 2009.
117.	Katsuhiko Hirota, Shizuo Kayama, Hiromichi Yumoto and Yoichiro Miyake : Intermedilysinに誘導されるHuCCT1細胞gp210の分布変動, 第62回日本細菌学会中国・四国支部総会, Oct. 2009.
118.	谷口友伯, Shizuo Kayama, 山本明毅, Tsuneko Ono, Katsuhiko Hirota, Yoichiro Miyake and Hideaki Nagamune : 緑膿菌のcyclic-di-GMPと表現型変化の関与について, 第62回日本細菌学会中国・四国支部総会, Oct. 2009.
119.	大平匡彦, Akihiro Shirai, Yoichiro Miyake, Hideaki Maseda and Hiroki Kourai : Synthesis and biological properties of strain-specific peptide derivative modified with a gemini quaternary ammonium salt, 第61回日本生物工学会大会, Sep. 2009.
120.	Katsuhiko Hirota, 稲垣覚, 濱田了, 石原和幸 and Yoichiro Miyake : 誘電泳動インピーダンス計測法を用いた迅速微生物数測定装置による口腔微生物の測定評価, 第51回歯科基礎医学会学術大会, Sep. 2009.
121.	Shizuo Kayama, Tsuneko Ono, 谷口友伯 and Yoichiro Miyake : 付着緑膿菌における抗菌薬抵抗性とcyclic-di-GMPの関与について, Bacterial Adherence & Biofilm第23回学術集会, Jul. 2009.
122.	天野富美夫 and Yoichiro Miyake : サルモネラおよび黄色ブドウ球菌の輸液チューブへの付着に及ぼす脂肪乳剤の影響, Bacterial Adherence & Biofilm第23回学術集会, Jul. 2009.
123.	相川文子, Shizuo Kayama, Keiji Murakami, Tsuneko Ono and Yoichiro Miyake : 緑膿菌の抗菌薬抵抗性におけるtcp遺伝子の役割, 四国歯学会第35回例会, Jun. 2009.
124.	Katsuhiko Hirota, Shizuo Kayama, Hiromichi Yumoto, Atsushi Tabata, Hideaki Nagamune, Tsuneko Ono and Yoichiro Miyake : Intermedilysinにより誘導される小胞体異常とgp210の細胞内分布の変動, 第82回日本日本細菌学会総会, Mar. 2009.
125.	谷口友伯, Shizuo Kayama, 山本明毅, Tsuneko Ono, Katsuhiko Hirota, Yoichiro Miyake and Hideaki Nagamune : 緑膿菌臨床分離株の付着表現型におけるcyclic-di-GMPの関与について, 第82回日本日本細菌学会総会, Mar. 2009.
126.	劉大力, 周彦王分, Hiromichi Yumoto, Shizuo Kayama, 李超倫, 梁景平, Yoichiro Miyake and 束蓉 : Purification of recombinant Porphyromonas gingivalis DnaK and creation of its mutant, 第82回日本日本細菌学会総会, Mar. 2009.
127.	山本明毅, Tsuneko Ono, Shizuo Kayama, 谷口友伯, Katsuhiko Hirota, Hideaki Nagamune and Yoichiro Miyake : 緑膿菌における抗菌薬抵抗性とQuorum sensing機構に関する研究 ∼蛍光染色法による解析∼, 第82回日本細菌学会総会, Mar. 2009.
128.	Shizuo Kayama, Tsuneko Ono, 山本明毅, 谷口友伯, Katsuhiko Hirota, Hideaki Nagamune and Yoichiro Miyake : 緑膿菌Quorum sensing機構とrpoS遺伝子がofloxacin抵抗性に及ぼす影響について, 第82回日本日本細菌学会総会, Mar. 2009.
129.	Shizuo Kayama, Tsuneko Ono and Yoichiro Miyake : 付着状態における緑膿菌とcyclic-di-GMPの関与について, 第43回緑膿菌感染症研究会, Feb. 2009.
130.	谷口友伯, Shizuo Kayama, Tsuneko Ono, 山本明毅, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌臨床分離株の付着時における抗菌薬抵抗性とcyclic-di-GMPの関与について, 第56回日本化学療法学会西日本支部総会, Dec. 2008.
131.	伊藤真理, Tsuneko Ono, Chieko Matsuura, Shizuo Kayama, Yoichiro Miyake, 山本明毅, 谷口友伯 and Hideaki Nagamune : 緑膿菌臨床分離株のバイオフィルム形成におけるcyclic-di-GMPの関与について, 第32回徳島県医学検査学会, Dec. 2008.
132.	田中純一, Tsuneko Ono, Katsuhiko Hirota, Shizuo Kayama and Yoichiro Miyake : Streptococcus intermediusに誘導される非アポトーシス細胞死の免疫組織化学的解析, 第32回徳島県医学検査学会, Dec. 2008.
133.	清宮明香, Chieko Matsuura, Tsuneko Ono, Shizuo Kayama, Yoichiro Miyake, 吉川和寛, 田尾知浩, 土田健司, 水口潤 and 川島周 : 透析液中の細菌検査法に関する遺伝学的検討, 第32回徳島県医学検査学会, Dec. 2008.
134.	山本明毅, Tsuneko Ono, Shizuo Kayama, 谷口友伯, Katsuhiko Hirota and Yoichiro Miyake : 緑膿菌における抗菌薬抵抗性の蛍光染色法による解析, 第61回日本細菌学会中国・四国支部総会, Oct. 2008.
135.	Katsuhiko Hirota, Shizuo Kayama, Atsushi Tabata, Hideaki Nagamune, Tsuneko Ono and Yoichiro Miyake : Intermedilysinにより誘導されるヒト培養胆管上皮細胞死とgp210の細胞内分布の変動, 第61回日本細菌学会中国・四国支部総会, Oct. 2008.
136.	Katsuhiko Hirota, Hirohiko Okamura, Kaya Yoshida, Tatsuji Haneji and Yoichiro Miyake : Streptococcus intermediusが誘導するヒト胆管上皮細胞死の分子機構の解明, 第50回歯科基礎医学会学術大会, Sep. 2008.
137.	Katsuhiko Hirota, Heni Susilowati, Hirohiko Okamura, Kaya Yoshida, Atsushi Tabata, Shizuo Kayama, Hiromichi Yumoto, Tatsuji Haneji, Hideaki Nagamune, Tsuneko Ono and Yoichiro Miyake : Intermedilysinにより誘導されるカルシウム振動と胆管上皮細胞死, 第17回Lancefielfレンサ球菌研究会 2008, Jul. 2008.
138.	Shizuo Kayama, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 付着状態における緑膿菌の抗菌薬抵抗性と遺伝子発現について, Bacterial Adherence & Biofilm第22回学術集会, Jul. 2008.
139.	清宮明香, Chieko Matsuura, Tsuneko Ono, Shizuo Kayama, Yoichiro Miyake, 道脇宏行, 英理香, 細谷陽子, 田尾知浩, 吉川和寛, 土田健司, 水口潤 and 川島周 : 透析液及び透析用水中の遺伝学的細菌検出法, 第53回日本透析医学会学術集会, Jun. 2008.
140.	Shizuo Kayama, 山本明毅, Ken Nemoto, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 緑膿菌Quorum sensing機構と抗菌薬抵抗性の蛍光染色法による解析, 第56回日本化学療法学会総会, Jun. 2008.
141.	木村智子, Kazumi Ozaki, Shizuo Kayama, 藤坂菊美, Hiromichi Yumoto and Yoichiro Miyake : Streptococcus mutansの病原性におけるスクロース輸送系遺伝子の役割について, 第81回日本細菌学会総会, Mar. 2008.
142.	木村智子, Kazumi Ozaki, Shizuo Kayama, 藤坂菊美 and Yoichiro Miyake : Streptococcus mutansの病原性におけるスクロース輸送系遺伝子の役割について, 第81回日本細菌学会総会, Mar. 2008.
143.	Shizuo Kayama, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 付着状態における緑膿菌の抗菌薬抵抗性と遺伝子発現について, 第81回日本細菌学会総会, Mar. 2008.
144.	Katsuhiko Hirota, Shizuo Kayama, Atsushi Tabata, Hideaki Nagamune, Tsuneko Ono and Yoichiro Miyake : Intermedilysinにより誘導される非アポトーシス型細胞死と細胞内Ca²⁺濃度の変動, 第81回日本細菌学会総会, Mar. 2008.
145.	Atsushi Tabata, Souichirou Miyoshi, Ayuha Kadota, Kou Fukushima, Akiko Sukeno, Toshifumi Tomoyasu, Katsuhiko Hirota, Yoichiro Miyake, Hisashi Ohkuni and Hideaki Nagamune : 細菌感染反応場としてのヒト型CD59:ヒト細胞指向性コレステロール依存性細胞溶解毒素の受容体及びStreptococcus intermedius感染の宿主側トリガー因子としての役割, 第80回日本生化学会大会-第30回日本分子生物学会年会合同大会, Dec. 2007. (Keyword) Streptococcus / CD59 / Infection
146.	清宮明香, Chieko Matsuura, Tsuneko Ono, Shizuo Kayama, Yoichiro Miyake, 道脇宏行, 英理香, 細谷陽子, 田尾知浩, 吉川和寛, 土田健司, 水口潤 and 川島周 : 透析液中の細菌検査法に関する遺伝学的検討, 第31回徳島県医学検査学会, Dec. 2007.
147.	安藤健一, 松田明日香, 竹内まりえ, 山本明毅, Chieko Matsuura, Tsuneko Ono, Shizuo Kayama and Yoichiro Miyake : 緑膿菌Quorum sensingのLas-system強制発現株の評価, 第31回徳島県医学検査学会, Dec. 2007.
148.	山本明毅, 安藤健一, 竹内まりえ, 松田明日香, Chieko Matsuura, Tsuneko Ono, Shizuo Kayama and Yoichiro Miyake : 緑膿菌におけるPQS Quorum sensingと抗菌薬抵抗性に関する研究, 第31回徳島県医学検査学会, Dec. 2007.
149.	松田明日香, 安藤健一, 竹内まりえ, 山本明毅, Chieko Matsuura, Tsuneko Ono, Shizuo Kayama and Yoichiro Miyake : 付着状態における緑膿菌の抗菌薬抵抗性とQuorum sensing機構について, 第31回徳島県医学検査学会, Dec. 2007.
150.	竹内まりえ, 松田明日香, 安藤健一, 山本明毅, Chieko Matsuura, Tsuneko Ono, Shizuo Kayama and Yoichiro Miyake : 緑膿菌Quorum sensingのLas-system強制発現株の作成, 第31回徳島県医学検査学会, Dec. 2007.
151.	Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake : The essentiality and involvements of Streptococcus intermedius histone-like protein in bacterial viability and normal growth, The 2nd international symposium on "The future direction of oral sciences in the 21st century", Dec. 2007.
152.	Katsuhiko Hirota, Susilowati Heni, 劉大力, Shizuo Kayama, Atsushi Tabata, Hideaki Nagamune, Tsuneko Ono and Yoichiro Miyake : Intermedilysinにより誘導されるヒト培養胆管上皮細胞死の分子機構の解明, 第60回日本細菌学会中国四国支部総会, Oct. 2007.
153.	Dali LIU, Keiji Murakami, Hiromichi Yumoto, Katsuhiko Hirota and Yoichiro Miyake : Investigation of the toxicity of Streptococcus intermedius histon-like protein in Escherichia coli, 第60回日本細菌学会中国・四国支部総会, Oct. 2007.
154.	Shizuo Kayama, Ken Nemoto, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 付着状態における緑膿菌の抗菌薬抵抗性とQuorum sensing機構について, 第55回日本化学療法学会西日本支部総会, Oct. 2007.
155.	Shizuo Kayama, Tsuneko Ono, Katsuhiko Hirota, 劉大力, Heni Susilowati and Yoichiro Miyake : 緑膿菌Quorum sensing機構とbiapenem抵抗性について, 第60回日本細菌学会中国・四国支部総会, Oct. 2007.
156.	Atsushi Tabata, Miyoshi Souichirou, Kadota Ayuha, Akiko Sukeno, Katsuhiko Hirota, Toshifumi Tomoyasu, Yoichiro Miyake and Hideaki Nagamune : Streptococcus intermedius が分泌する細胞溶解毒素インターメディリシンが関与するヒト細胞特異的な感染現象, 第54回毒素シンポジウム, Sep. 2007. (Keyword) cytotoxicity / Streptococcus
157.	Dali LIU, Hiromichi Yumoto, Takashi Matsuo and Yoichiro Miyake : Histone-like DNA binding protein of Streptococcus intermedius induces the expression of pro-inflammatory cytokines in human monocytes via activation of ERK1/2 and JNK pathways., 日本歯周病学会50周年記念大会, Sep. 2007.
158.	Katsuhiko Hirota, Hirohiko Okamura, Kaya Yoshida, Tatsuji Haneji and Yoichiro Miyake : Intermedilysin が誘導する非アポトーシス型細胞死の分子機構の解明, 第49 回歯科基礎医学会総会, Aug. 2007.
159.	Yoichiro Miyake : 教育講演IV 口腔内での細菌付着について, Bacterial Adherence & Biofilm第21回学術集会, Jul. 2007.
160.	Shizuo Kayama, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 付着状態における緑膿菌の抗菌薬抵抗性とQuorum sensing機構について, Bacterial Adherence & Biofilm第21回学術集会, Jul. 2007.
161.	Atsushi Tabata, 三好宗一郎, Toshifumi Tomoyasu, Katsuhiko Hirota, Yoichiro Miyake and Hideaki Nagamune : 細胞溶解毒素インターメディリシンのヒト細胞特異的感染因子としての役割, 第80回日本細菌学会総会, Mar. 2007.
162.	今大路浩之, Katsuhiko Hirota, Yoichiro Miyake and Tomomi Kuwahara : Bacteroides fragilisのDNA逆位による外膜vesicle形成の制御, 第80回日本細菌学会総会, Mar. 2007.
163.	LIU Dali, Keiji Murakami, Hiromichi Yumoto, Katsuhiko Hirota and Yoichiro Miyake : Investigation of the essentiality of histone-like protein in Streptococcus intermedius, 第80回日本細菌学会総会, Mar. 2007.
164.	Shizuo Kayama, Keiji Murakami, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 付着緑膿菌の抗菌薬抵抗性とQuorum sensing機構について, 第80回日本細菌学会総会, Mar. 2007.
165.	Katsuhiko Hirota, LIU Dali, Shizuo Kayama, Keiji Murakami, Hideaki Nagamune, Tsuneko Ono and Yoichiro Miyake : Intermedilysinによる培養ヒト胆管上皮細胞核膜抗原の異所性表出, 第80回日本細菌学会総会, Mar. 2007.
166.	劉大力, Keiji Murakami, Hiromichi Yumoto, Katsuhiko Hirota and Yoichiro Miyake : Investigation of the Essentiality of Histone-Like Protein in Streptococcus intermedius, 第80回日本細菌学会総会, Mar. 2007.
167.	野間保喜, 堀川明日香, 滝本真由美, 玉井利奈, Chieko Matsuura, Tsuneko Ono, Keiji Murakami and Yoichiro Miyake : う蝕原因菌Streptococcus mutansの病原性に関する研究, 第30回徳島県医学検査学会, Dec. 2006.
168.	玉井利奈, 滝本真由美, 野間保喜, 堀川明日香, Chieko Matsuura, Tsuneko Ono, Keiji Murakami and Yoichiro Miyake : 緑膿菌臨床分離株における抗菌薬感受性とバイオフィルム形成に関する研究, 第30回徳島県医学検査学会, Dec. 2006.
169.	滝本真由美, 玉井利奈, 堀川明日香, 野間保喜, Chieko Matsuura, Tsuneko Ono, Keiji Murakami and Yoichiro Miyake : 緑膿菌におけるPQS Quorum Sensingと病原性に関する研究, 第30回徳島県医学検査学会, Dec. 2006.
170.	堀川明日香, 野間保喜, 滝本真由美, 玉井利奈, Chieko Matsuura, Tsuneko Ono, Keiji Murakami and Yoichiro Miyake : 抗菌薬添加によるQuorum Sensing関連遺伝子発現の変動, 第30回徳島県医学検査学会, Dec. 2006.
171.	Keiji Murakami, Shizuo Kayama, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 抗菌薬添加による緑膿菌Quorum Sensing関連遺伝子発現の変化, 第54回日本化学療法学会西日本支部総会, Dec. 2006.
172.	木村智子, Kazumi Ozaki, 藤坂菊美, Keiji Murakami and Yoichiro Miyake : Streptococcus mutansの病原性におけるスクロース輸送系遺伝子の役割について, 第59回日本細菌学会中国・四国支部総会, Oct. 2006.
173.	吉嶋佑佳, Keiji Murakami, Katsuhiko Hirota and Yoichiro Miyake : 親水性コーティング法による細菌付着抑制効果の検討, 第59回日本細菌学会中国・四国支部総会, Oct. 2006.
174.	LIU Dali, Hiromichi Yumoto, Kanako Takahashi, Keiji Murakami, Katsuhiko Hirota and Yoichiro Miyake : Proinflammatory cytokines are induced in human monocytes in response to exposure to the histone like protein of Streptococcus intermedius, 第59回日本細菌学会中国・四国支部総会, Oct. 2006.
175.	Katsuhiko Hirota, LIU Dali, Shizuo Kayama, Keiji Murakami, Hideaki Nagamune and Yoichiro Miyake : Intermedilysinによるlamin B1の異所性表出, 第59回日本細菌学会中国・四国支部総会, Oct. 2006.
176.	板野守秀, 矢納義高, 吉田修, Katsuhiko Hirota and Yoichiro Miyake : 細菌共凝集塊の物理的分離におよぼす糖アルコール類の影響, 第48回歯科基礎医学会学術大会, Sep. 2006.
177.	Katsuhiko Hirota, Hirohiko Okamura, Kaya Yoshida, Tatsuji Haneji and Yoichiro Miyake : Aberrant cell surface localization of the nuclear antigen in HuCCT1 cell by Intermedilysin, 第48回歯科基礎医学会学術大会, Sep. 2006.
178.	Katsuhiko Hirota, Hirohiko Okamura, Kaya Yoshida, Tatsuji Haneji and Yoichiro Miyake : Intermedilysin によるHuCCT1 細胞核抗原の異所性表出, 第48回歯科基礎医学会総会, Sep. 2006.
179.	Shizuo Kayama, Keiji Murakami, Katsuhiko Hirota, Tsuneko Ono, Hideaki Nakae and Yoichiro Miyake : 付着緑膿菌の抗菌薬抵抗性とQuorum sensing機構について, Bacterial Adherence & Biofilm 第20回記念学術集会, Jul. 2006.
180.	Keiji Murakami, Shizuo Kayama, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 抗菌薬抵抗性による緑膿菌rpoS遺伝子発現の変化, 第54回日本化学療法学会総会, May 2006.
181.	Shizuo Kayama, Keiji Murakami, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 緑膿菌Quorum sensingとofloxacin抵抗性について, 第79回日本細菌学会総会, Mar. 2006.
182.	Keiji Murakami, Shizuo Kayama, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 抗菌薬による緑膿菌rpoS遺伝子プロモーター活性への影響, 第79回日本細菌学会総会, Mar. 2006.
183.	Keiji Murakami, 鹿山鎭男, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 緑膿菌Quorum sensingとOfloxacin抵抗性について, 第53回日本化学治療学会西日本支部総会, Dec. 2005.
184.	Keiji Murakami, 鹿山鎭男, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 緑膿菌 tcp 遺伝子が抗菌薬抵抗性に及ぼす影響について, 第58回日本細菌学会中国・四国支部総会, Oct. 2005.
185.	Oheni Susilowati, Katsuhiko Hirota, Dali Liu, Sizuo Kayama, Keiji Murakami, Ken Kikuchi, Kyoichi Totsuka, Hideaki Nagamune and Yoichiro Miyake : The Mechanism of Bile Epithelium Cell Death Under the Stimulation of Streptococcus Intermedius Cytolysin, 第58回日本細菌学会中国・四国支部総会, Oct. 2005.
186.	田中宏明, Hirohiko Okamura, Katsuhiko Hirota, 尾崎明子, Bruna Rabelo Amorim, Kaya Yoshida, 長宗秀明, Yoichiro Miyake, Toshihiko Nagata and Tatsuji Haneji : Streptococcus Intermedius由来毒素intermedilysin処理細胞におけるPTEN, Akt, 及びPDK-1の発現, 第47回歯科基礎医学会総会, Sep. 2005.
187.	Yoichiro Miyake : 教育セミナー Biofilm:基礎研究から臨床応用へ, Bacterial Adherence & Biofilm 第19回学術集会, Jul. 2005.
188.	Keiji Murakami, Shizuo Kayama, Ken Nemoto, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 緑膿菌tcp遺伝子と抗菌薬抵抗性について, 第53回化学療法学会総会, May 2005.
189.	鹿山鎭男, Keiji Murakami, Ken Nemoto, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : P1-202 緑膿菌 lasl, rhll 遺伝子と抗菌薬抵抗性について, 第78回日本細菌学会総会, Apr. 2005.
190.	Keiji Murakami, 鹿山鎭男, Ken Nemoto, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : P1-192 緑膿菌 bta 遺伝子の抗菌薬抵抗性への影響について, 第78回日本細菌学会総会, Apr. 2005.
191.	鹿山鎭男, Keiji Murakami, Darija Viducic, 劉大力, Ken Nemoto, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 緑膿菌 lasl, rhll 遺伝子と抗菌薬抵抗性について, 第52回日本化学治療法学会西日本支部総会, Dec. 2004.
192.	Katsuhiko Hirota, 菊池賢, 劉大力, 鹿山鎭男, Keiji Murakami, Ken Nemoto, Yoichiro Miyake and Tsuneko Ono : 脳腫瘍以来のS.intermedius ILY高産生株に対するカルバベネム系抗菌薬感受性に関する検討, 第52回日本化学治療法学会西日本支部総会, Dec. 2004.
193.	鹿山鎭男, Keiji Murakami, Darija Viducic, 劉大力, Ken Nemoto, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 緑膿菌 Quorum Sensing 機構と抗菌薬抵抗性について, 第57回日本細菌学会中国・四国支部総会, Oct. 2004.
194.	Katsuhiko Hirota, 劉大力, 鹿山鎭男, Keiji Murakami, Ken Nemoto, Akiko Sukeno, Hideaki Nagamune, 菊池賢, 戸塚恭一 and Yoichiro Miyake : 抗Streptococcus intermedius HU型ヒストン様タンパク質抗体のHepG2細胞内ミトコンドリアとの反応性, 第57回日本細菌学会中国・四国支部総会, Oct. 2004.
195.	Katsuhiko Hirota, 劉大力, 鹿山鎭男, Keiji Murakami, Ken Nemoto, Tsuneko Ono and Yoichiro Miyake : Anginosus group streptococci感染症とその続発生, 第2回感染病態研究会シンポジウム「Symbiosis」, Jun. 2004.
196.	Keiji Murakami, Shizuo Kayama, Darija Viducic, Ken Nemoto, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 緑膿菌PAO1株におけるbiapenem抵抗性変異株の分離, 第52回日本化学療法学会総会, Jun. 2004.
197.	Akiko Sukeno, Hideaki Nagamune, Katsuhiko Hirota, Kazuto Ohkura, Takuya Maeda, Yoichiro Miyake and Hiroki Kourai : インターメディリシンはS. intermediusがHepG2細胞に侵入する際に必要不可欠な因子である, 日本細菌学会第77回総会, Apr. 2004.
198.	榊原恵美子, Hideaki Nagamune, Keiji Murakami, Takuya Maeda, Yoichiro Miyake and Hiroki Kourai : 緑膿菌外膜タンパク質OprRの抗生物質感受性への関与, 日本細菌学会第77回総会, Apr. 2004.
199.	Keiji Murakami, 鹿山鎭男, Ken Nemoto, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 付着緑膿菌の抗菌薬抵抗性変異株の分離, 第77回日本細菌学会総会, Apr. 2004.
200.	Hideaki Nagamune, 榊原恵美子, 田端厚之, Takuya Maeda, Hiroki Kourai, Keiji Murakami and Yoichiro Miyake : 第四アンモニウム塩系消毒剤に対する緑膿菌の耐性化には外膜蛋白質OryRが関与している, 日本防菌防黴学会第30回年次大会, May 2003.
201.	Hideaki Nagamune, Takuya Maeda, Keiji Murakami, Yoichiro Miyake and Hiroki Kourai : 第四アンモニウム塩系消毒剤に対する緑膿菌の耐性化機構:外膜タンパク質QRP01の関与, 日本細菌学会第76回総会, Apr. 2003.
202.	Hiromichi Yumoto, Katsuhiko Hirota, Hideaki Nagamune, Kazuto Ohkura, Keiji Murakami, 山田真義, Hideaki Nakae and Yoichiro Miyake : Streptococcus intermedius hitone-like proteinをコードする遺伝子のクローニングと発現, 第75回日本細菌学会総会, Apr. 2002.
203.	Hiromichi Yumoto, Katsuhiko Hirota, 山田真義, Hideaki Nakae, Keiji Murakami, Yoichiro Miyake and Takashi Matsuo : Streptococcus intermedius histone-like proteinをコードする遺伝子のクローニングと発現, 第44回秋季日本歯周病学会学術大会, Oct. 2001.
204.	村田広美, Katsuhiko Hirota, Mizuho Nishino and Yoichiro Miyake : 前思春期性歯周炎の1症例, 第39回日本小児歯科学会大会および総会, May 2001.
205.	Katsuhiko Hirota, Hiromichi Yumoto, 村田広美, Kazuto Ohkura, Hideaki Nagamune, Ken Nemoto, Tsuneko Ono and Yoichiro Miyake : レンサ球菌のヒストン様蛋白質の構造解析, 第53回日本細菌学会中国・四国支部総会, Oct. 2000.

Et cetera, Workshop:

1.	Takashi Amoh, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Katsuhiko Hirota, Yoichiro Miyake and Hideki Fujii : 幼児口腔内から検出された多剤耐性レンサ球菌について, 第71回日本細菌学会中国，四国支部総会, Oct. 2018.
2.	Keiji Murakami, 狩山玲子, 公文裕巳 and Yoichiro Miyake : 緑膿菌Autoinducer Analogがマクロライドの抗菌作用に及ぼす影響について, 第52回緑膿菌感染症研究会講演記録, Vol.52, 67, Oct. 2018.
3.	Shinichi Fujie, Shoto Ogawa, Keiji Murakami, Takashi Amoh, Yoichiro Miyake, 佐藤雅美, 笹田倫子, 菅崎幹樹, 香川葉子, Yuri Morimoto, Momoyo Azuma, Akiko Sakurai and Keiko Kataoka : 呼吸器由来緑膿菌における病原性と抗菌薬抵抗性との関連について, 第40回徳島県医学検査学会, Dec. 2016.
4.	Keiko Kataoka, 南亜利沙, Keiji Murakami, Yoichiro Miyake, 畑美智子, 佐藤雅美, 岡本貴子, 香川葉子, 菅崎幹樹 and Momoyo Azuma : 本院で分離されたMRSAの分類とその特徴, 徳島県医学検査学会, Dec. 2013.
5.	Keiko Kataoka, 堀口なつみ, Keiji Murakami, Yoichiro Miyake, 畑美智子, 佐藤雅美, 岡本貴子, 香川葉子, 菅崎幹樹 and Momoyo Azuma : 「緑膿菌臨床分離株における抗菌薬抵抗性について」, 徳島県医学検査学会, Dec. 2013.
6.	Keiji Murakami, Momoyo Azuma, 畑美智子, 長尾多美子, 鈴木麗子, Yoichiro Miyake and Shoji Sakiyama : 徳島大学病院におけるパルスフィールドゲル電気泳動法(PFGE)によるMRSAの分子疫学調査, 第4回MRSAフォーラム, Jul. 2013.
7.	Hisahiro Takeuchi, Toshiyuki Suge, Yukihiro Momota, Kazumi Ozaki, Atsuko Nakamichi, Yoichiro Miyake, Katsuhiko Yoshimoto and Tetsuo Ichikawa : 日本歯科大学臨床実習視察報告, Shikoku Dental Research, Vol.24, No.2, 85-94, Jan. 2012. (Tokushima University Institutional Repository) ● Metadata: 109821 (Tokushima University Institutional Repository: 109821)
8.	Kan Nagao, Tomoko Kimura, Akihiro Yasue, Yoichiro Miyake, Katsuhiko Yoshimoto and Tetsuo Ichikawa : 東京医科歯科大学歯学部歯学科の臨床実習視察報告, Shikoku Dental Research, Vol.24, No.2, 79-84, Jan. 2012. (Tokushima University Institutional Repository) ● Metadata: 109820 (Tokushima University Institutional Repository: 109820)
9.	Atsushi Tabata, 山本泰裕, Toshifumi Tomoyasu, Katsuhiko Hirota, Yoichiro Miyake, 大国寿士 and Hideaki Nagamune : 2種のコレステロール依存性細胞溶解毒素を産生する川崎病患児由来Streptococcus mitis Nm-65株の病原性に関する検討, 第19回Lancefieldレンサ球菌研究会, Jun. 2010.
10.	Katsuhiko Hirota, 米山武義, 栗原正紀, 宮本寛, 坂本まゆみ, Keiji Murakami and Yoichiro Miyake : 摂食嚥下障害のある脳血管障害患者と緑膿菌, はち・まる・にい・まる 8020, No.7, 108-109, Jan. 2008.
11.	Yoichiro Miyake : 口腔ケアの基礎知識, 8020運動推進特別事業における研修会, Oct. 2007.
12.	Shizuo Kayama, Keiji Murakami, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 付着緑膿菌の抗菌薬抵抗性とQuorum sensing機構について, Bacterial Adherence & Biofilm, Vol.20, 110-115, Jul. 2006.
13.	Katsuhiko Hirota and Yoichiro Miyake : アンギノーサス群レンサ球菌とは?, Dental Outlook, Vol.100, No.6, 1348-1349, Dec. 2002.
14.	Keiji Murakami and Yoichiro Miyake : バイオフィルムにおける遺伝子発現とクオラムセンシング, Dental Outlook, Vol.100, No.3, 612-613, Sep. 2002.
15.	Yoichiro Miyake : 口腔細菌のクオラムセンシング, Dental Outlook, Vol.99, No.6, 1322-1323, Jun. 2002.
16.	Katsuhiko Hirota, Hiromichi Yumoto, 村田広美, Viducic Darijia, 谷口和子, Keiji Murakami, Ken Nemoto, Tsuneko Ono and Yoichiro Miyake : Staphyrococcus aureusにおけるhistone-like proteinの存在と構造解析, 第46回ブドウ球菌研究会, Sep. 2001.
17.	谷口和子, Keiji Murakami, Tsuneko Ono, Katsuhiko Hirota, Ken Nemoto and Yoichiro Miyake : 緑膿菌におけるBIPM抵抗性変異株の作成, Bacterial Adherence & Biofilm, Vol.15, 22-26, Jan. 2001.
18.	Keiji Murakami, 谷口和子, Darija Viducic, Ken Nemoto, Katsuhiko Hirota, Tsuneko Ono and Yoichiro Miyake : 緑膿菌抗菌薬抵抗性におけるストレス反応の影響, Bacterial Adherence & Biofilm, Vol.15, 27-30, Jan. 2001.
19.	足立敦, 鴨井久一, Yoichiro Miyake and 遊佐典子 : 臨床検査を歯科医療にどう活かすか, Dental Outlook, Vol.96, No.6, 1206-1223, Dec. 2000.
20.	Yoichiro Miyake and 谷口和子 : 唾液と口腔微生物, Journal of Clinical Pediatric Dentistry, Vol.5, No.2, 28-36, Feb. 2000.
21.	石川昭, 米山武義, Yoichiro Miyake and 宮武光吉 : 口腔ケアによる咽頭細菌数の変動, The Japanese Journal of Nursing Arts, Vol.46, No.1, 82-86, Jan. 2000.
22.	Katsuhiko Hirota, 村田広美, Hiromichi Yumoto, 大倉一人, Hideaki Nagamune and Yoichiro Miyake : レンサ球菌のヒストン様蛋白質の構造解析, Bacterial Adherence & Biofilm, Vol.14, 6-8, Jan. 2000.
23.	Keiji Murakami, Tsuneko Ono, 谷口和子, Takashi Matsuo and Yoichiro Miyake : 付着緑膿菌における抗菌薬抵抗性変異株の分離とその性状, Bacterial Adherence & Biofilm, Vol.13, 23-25, Jan. 1999.
24.	Yoichiro Miyake : 誤嚥性肺炎の発症における口腔細菌の役割と細菌学的にみた口腔ケアの意義, Dental Outlook, Vol.91, No.6, 1298-1303, Jun. 1998.
25.	Katsuhiko Hirota and Yoichiro Miyake : 小児の口腔内病原微生物と感染防止策, Journal of Clinical Pediatric Dentistry, Vol.3, No.1, 29-40, Jan. 1998.
26.	村上欣也, Katsuhiko Hirota, Ken Nemoto, Tsuneko Ono, Takashi Matsuo and Yoichiro Miyake : Streptococcus intermediusの抗sialyl-Lewis^x (CD15s)抗体誘導能, Bacterial Adherence & Biofilm, Vol.11, 14-20, Jan. 1997.
27.	Katsuhiko Hirota and Yoichiro Miyake : アパタイトインプラントの細菌学的考察, DE, Vol.116, 34-37, Jan. 1996.
28.	Katsuhiko Hirota, Tetsuo Ichikawa, Toshiya Kashiwabara, Ken Nemoto, Tsuneko Ono, 村上欣也, Takashi Matsuo, 松本直之 and Yoichiro Miyake : Candida albicans biofilm modelの作製と電解水の抗biofilm効果, Bacterial Adherence & Biofilm, Vol.10, 30-35, Jan. 1996.

Report:

1.	Tsuneko Ono, Yoichiro Miyake, Katsuhiko Hirota and Ken Nemoto : 付着細菌における抗菌薬抵抗性のメカニズムの解析, 平成12年度∼13年度科学研究費補助金(基盤研究(C2)研究成果報告書,
2.	Yoichiro Miyake, 佐藤博信, 中田真吾, 村田真里, 倉治竜太郎, 小口莉代, 金子友希乃 and 武内一広 : 学生参加による歯科医学教育カリキュラム改革, The Journal of Japanese Dental Education Association, Vol.27, No.3, 211-214, 2011.
3.	宮武光吉, Yoichiro Miyake, 飯島理, 糟谷正 and 神川正 : 社会福祉施設等入所者口腔内状態改善研究モデル事業報告書, Hamamatsu, Mar. 1999.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

A study on oxidative stress as bactericidal mechanism of antibacterial agents by metabolome analysis (Project/Area Number: 16K11446 )
The mechanisms of antibiotic tolerance focusing on intracellular signaling substance c-di-GMP. (Project/Area Number: 26462787 )
Development of oral probiotics using multi-bacteria (Project/Area Number: 25670886 )
Molecular characterization of the pathogenic factors of oral streptococci involved in autoimmune disease (Project/Area Number: 24592833 )
The screening for antibiotic tolerance related genes in peg-biofilm cells of Pseudomonas aeruginosa (Project/Area Number: 22592040 )
The study of oral streptococcal pathogenicity of autoimmune hepatitis (Project/Area Number: 21592387 )
Regulatory Network for Expression of Antibiotic Tolerance in Pseudomonas aeruginosa (Project/Area Number: 21590627 )
Development of new and safer denture cleanser with Erythritol (Project/Area Number: 20592271 )
The relationship between biofiim formation and antibiotio toIerance in Pseudomonas aeruginosa (Project/Area Number: 19592115 )
The effect of intermedilysin on human bile duct cells (Project/Area Number: 18592003 )
Molecular analysis of anteliotic-tolerance and stress response in adherent bacteria (Project/Area Number: 17591914 )
The characterization of DNA-binding protein from Streptococcus intermediusand elucidate its virulence mechanism (Project/Area Number: 14571788 )
A study Of ora| streptococcal histone-like protein and virulence mechanism (Project/Area Number: 12671827 )
The Mechanism of Antibiotic-tolerance in Adherent bacteria (Project/Area Number: 12671774 )
A stady of tannin-protein comprex degrading enteric bacteria isolated from herbivores. (Project/Area Number: 11660297 )
Examine pathogenicity of oral Streptococcus spp. by its surface antigens on liver disease (Project/Area Number: 10671768 )
The Mechanism of Antibiotic-Resistance in Oral Streptococci (Project/Area Number: 10671703 )
A study of oral streptococci on the dherence and resistance to human immune system and antibacterial agents (Project/Area Number: 09671857 )
口腔連鎖球菌の病原性および薬剤耐性機構の解明 (Project/Area Number: 08672074 )
Immunological analysis of a cell surface antigen by a monoclonal antibody SNH-3 in oral streptococci (Project/Area Number: 06672059 )
Diagnosis of streptococcal infection by PCR amplification (Project/Area Number: 06671815 )
バイオフィルム細菌の抗生物質および消毒剤抵抗性に関する研究 (Project/Area Number: 06671813 )
口腔ブドウ球菌の病原性およびその付着機構 (Project/Area Number: 05671571 )
メチシリン耐性黄色ブドウ球菌(MRSA)の薬剤耐性機構と病原性 (Project/Area Number: 05454510 )
局所ドラッグデリバリーシステムによる歯周治療薬の開発 (Project/Area Number: 04557801 )
The development of chemotherapoetic agents for periodontitis utilizing local drug delivery system. (Project/Area Number: 04557081 )
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Professor Emeritus : Miyake, Yoichiro

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)