Tokushima University / Educator and Researcher Directory --- Yokoigawa, Kumio

Personal Web Page

https://researchmap.jp/read0119878 (researchmap)

Field of Study

○	Applied Microbiology

Subject of Study

○	Structure and function of psychrophilic enzymes, Sterilization of pathogenic bacteria amd reduction of pathogenicity (psychrophilic enzymes, Escherichia coli O157, amino acid racemase) (Enzymological study on psychrophilic enzymes, and sterilization of pathogenic bacteria and reduction of pathogenicity.)

Book / Paper

Book:

1.	Kumio Yokoigawa : 食物科学概論改訂版食品の安全性微生物による食中毒, 朝倉書店, Tokyo, Mar. 2014.
2.	Kumio Yokoigawa : 5.食物の安全性 5.2 微生物による食中毒, 朝倉書店, Tokyo, Nov. 2003.
3.	Kumio Yokoigawa and 健次左右田 : 5章酵素的合成 5-5 その他, The Japanese Biochemical Society, Tokyo, May 1993.

Academic Paper (Judged Full Paper):

1.	Hitomi Sakai, Tohru Sakai, Badr Ali Hoida, Kaori Kanemaru and Kumio Yokoigawa : Analysis of cereal extracts as conditioning solutes to suppress the initial attachment of Escherichia coli to abiotic surfaces, European Food Research and Technology, 2020. (Keyword) Cereals / Escherichia coli / Attachment / Abiotic materials (Tokushima University Institutional Repository) ● Metadata: 114680 (Link to Publication Site) ● Publication site (DOI): 10.1007/s00217-020-03444-9 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85081030075 (Tokushima University Institutional Repository: 114680, DOI: 10.1007/s00217-020-03444-9, Elsevier: Scopus)
2.	Yumiko Nishimura-Danjobara, Keisuke Oyama, Tomohiro Oyama, Kumio Yokoigawa and Yasuo Oyama : Modification of cell vulnerability to oxidative stress by N-(3-oxododecanoyl)-L-homoserine-lactone, a quorum sensing molecule, in rat thymocytes, Chemico-Biological Interactions, Vol.302, 143-148, 2019. (Summary) N-(3-oxododecanoyl)-L-homoserine-lactone (ODHL), a quorum sensing molecule, affects intracellular Zn2+ concentration ([Zn2+]i) and cellular levels of nonprotein thiols ([NPT]i) of rat thymic lymphocytes, both of which are assumed to affect cell vulnerability to oxidative stress. Therefore, it is interesting to examine the effects of ODHL on the cells under oxidative stress. ODHL augmented the cytotoxicity of H2O2, but not calcium ionophore A23187. ODHL potentiated the H2O2-induced elevation of [Zn2+]i, wherein, it greatly attenuated the H2O2-induced increase in intracellular Ca2+ concentration. ODHL did not affect [NPT]i in the presence of H2O2. Therefore, we conclude that the elevation of [Zn2+]i is involved in the ODHL-induced potentiation of H2O2 cytotoxicity. Our findings suggest that ODHL modifies cell vulnerability to oxidative stress in host cells. (Tokushima University Institutional Repository) ● Metadata: 113065 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.cbi.2019.02.006 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.cbi.2019.02.006 (Tokushima University Institutional Repository: 113065, DOI: 10.1016/j.cbi.2019.02.006)
3.	Honoka Wada, Keisuke Oyama, Risa Kamae, Toshiya Masuda, Kaori Kanemaru, Kumio Yokoigawa and Yasuo Oyama : Zinc-dependent and independent actions of hydroxyhydroquinone on rat thymic lymphocytes, Drug and Chemical Toxicology, Vol.9, 1-6, 2018. (Summary) Coffee contains hydroxyhydroquinone (HHQ). HHQ is one of by-products released during bean roasting. Therefore, it is important to elucidate the bioactivity of HHQ to predict its beneficial or adverse effects on humans. We studied zinc-dependent and independent actions of commercially-procured synthetic HHQ in rat thymocytes using flow cytometric techniques with propidium iodide, FluoZin-3-AM, 5-chloromethylfluorescein diacetate, and annexin V-FITC. HHQ at 10¬50 µM elevated intracellular Zn2+ levels by releasing intracellular Zn2+. HHQ at 10 µM increased cellular thiol content in a Zinc-dependent manner. However, HHQ at 30 50 µM reduced cellular thiol content. Although the latter actions of HHQ (30 50 µM) were suggested to increase cell vulnerability to oxidative stress, HHQ at 0.3 100 µM significantly protected cells against oxidative stress induced by H2O2. The process of cell death induced by H2O2 was delayed by HHQ, although both H2O2 and HHQ increased the population of annexin V-positive living cells. However, HHQ at 10 30 µM promoted cell death induced by A23187, a calcium ionophore. HHQ at 10 30 µM exerted contrasting effects on cell death caused by oxidative stress and Ca2+ overload. Because HHQ is considered to possess diverse cellular actions, coffee with reduced amount of HHQ may be preferable to avoid potential adverse effects. (Keyword) Zinc / Hydroyxhydroquinone (Tokushima University Institutional Repository) ● Metadata: 111979 (Link to Publication Site) ● Publication site (DOI): 10.1080/01480545.2018.1429462 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1080/01480545.2018.1429462 (Tokushima University Institutional Repository: 111979, DOI: 10.1080/01480545.2018.1429462)
4.	Yumiko Nishimura-Danjobara, Keisuke Oyama, Kumio Yokoigawa and Yasuo Oyama : Hyperpolarization by N-(3-oxododecanoyl)-L-homoserine-lactone, a quorum sensing molecule, in rat thymic lymphocytes, Chemico-Biological Interactions, Vol.283, 91-96, 2018. (Summary) To study the adverse effects of N-(3-oxododecanoyl)-L-homoserine-lactone (ODHL), a quorum sensing molecule, on mammalian host cells, its effect on membrane potential was examined in rat thymic lymphocytes using flow cytometric techniques with a voltage-sensitive fluorescent probe. As 3 300 µM ODHL elicited hyperpolarization, it is likely that it increases membrane K+ permeability because hyperpolarization is directly linked to changing K+ gradient across membranes, but not Na+ and Cl- gradients. ODHL did not increase intracellular Ca2+ concentration. ODHL also produced a response in the presence of an intracellular Zn2+ chelator. Thus, it is unlikely that intracellular Ca2+ and Zn2+ are attributed to the response. Quinine, a non-specific K+ channel blocker, greatly reduced hyperpolarization. However, because charybdotoxin, tetraethylammonium chloride, 4-aminopyridine, and glibenclamide did not affect it, it is pharmacologically hypothesized that Ca2+-activated K+ channels, voltage-gated K+ channels, and ATP-sensitive K+ channels are not involved in ODHL-induced hyperpolarization. Although the K+ channels responsible for ODHL-induced hyperpolarization have not been identified, it is suggested that ODHL can elicit hyperpolarization in mammalian host cells, disturbing cellular functions. (Keyword) 4-Butyrolactone / Animals / Calcium / Cell Polarity / Charybdotoxin / Flow Cytometry / Glyburide / Homoserine / KATP Channels / Lymphocytes / Permeability / Potassium / Potassium Channels, Voltage-Gated / Quinine / Quorum Sensing / Rats / Rats, Wistar / Thymocytes (Tokushima University Institutional Repository) ● Metadata: 111985 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.cbi.2018.02.008 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29427588 ● Search Scopus @ Elsevier (PMID): 29427588 ● Search Scopus @ Elsevier (DOI): 10.1016/j.cbi.2018.02.008 (Tokushima University Institutional Repository: 111985, DOI: 10.1016/j.cbi.2018.02.008, PubMed: 29427588)
5.	Kaori Kanemaru, Goto Tsukie, Badr Ali Hoida and Kumio Yokoigawa : Determination of binding affinity of poly-gamma-glutamate to Shiga toxin, Journal of Food Biochemistry, Vol.42, No.5, e12538, 2018. (Keyword) Escherichia coli O157:H7 / Poly-gamma-glutamate / Shiga toxin (Tokushima University Institutional Repository) ● Metadata: 113899 (Link to Publication Site) ● Publication site (DOI): 10.1111/jfbc.12538 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1111/jfbc.12538 (Tokushima University Institutional Repository: 113899, DOI: 10.1111/jfbc.12538)
6.	Yumiko Nishimura-Danjobara, Keisuke Oyama, Kaori Kanemaru, Keiko Takahashi, Kumio Yokoigawa and Yasuo Oyama : N-(3-oxododecanoyl)-L-homoserine-lactone, a quorum sensing molecule, affects cellular content of nonprotein thiol content in rat lymphocytes: Its relation with intracellular Zn2+, Chemico-Biological Interactions, Vol.280, 28-32, 2018. (Summary) Cellular actions of N-(3-oxododecanoyl)-L-homoserine-lactone (ODHL), a quorum sensing molecule of bacteria, were studied on rat thymocytes using a flow cytometer with appropriate fluorescent dyes to elucidate the effects of ODHL on host cells. A bell-shaped concentration-response relation was observed in the ODHL-induced changes in cellular glutathione content ([GSH]i). ODHL concentration-dependently increased intracellular Zn2+ levels ([Zn2+]i) and cellular O2- content ([O2-]i). The bell-shaped relation induced by ODHL can be explained as follows: a low concentration of ODHL is expected to induce moderate oxidative stress that intracellularly releases Zn2+ by converting thiols to disulfides. A slight elevation of [Zn2+]i may increase the [GSH]i. On the other hand, it is likely that a high concentration of ODHL causes severe oxidative stress that further causes both the decrease in [GSH]i and the increase in [Zn2+]i. Excessive increase in [Zn2+]i may augment oxidative stress that further decreases the [GSH]i. Other notable actions induced by ODHL included the elevation of [Zn2+]i by Zn2+ influx and the increase in [GSH]i under Zn2+-free conditions. Therefore, it is suggested that ODHL elicits diverse actions on host cells. (Keyword) 4-Butyrolactone / Animals / Apoptosis / Cells, Cultured / Glutathione / Homoserine / Lymphocytes / Male / Oxidative Stress / Quorum Sensing / Rats / Rats, Wistar / Sulfhydryl Compounds / Thymus Gland / Zinc (Tokushima University Institutional Repository) ● Metadata: 111976 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.cbi.2017.12.013 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29223568 ● Search Scopus @ Elsevier (PMID): 29223568 ● Search Scopus @ Elsevier (DOI): 10.1016/j.cbi.2017.12.013 (Tokushima University Institutional Repository: 111976, DOI: 10.1016/j.cbi.2017.12.013, PubMed: 29223568)
7.	Norio Kamemura, Keisuke Oyama, Kaori Kanemaru, Kumio Yokoigawa and Yasuo Oyama : Diverse cellular actions of tert-butylhydroquinone, a food additive, on rat thymocytes, Toxicology Research, Vol.6, 922-929, 2017. (Summary) Tertiary butylhydroquinone (TBHQ) is a food additive that possesses antioxidant activity. Its alternative applications have been explored in recent studies. However, there is controversy regarding safety. In this study using rat thymocytes, the cellular actions of TBHQ at sublethal concentrations were examined. TBHQ at concentrations of 3 µM or more elevated intracellular Zn2+ concentration ([Zn2+]i) in a dose-dependent manner, by increasing membrane Zn2+ permeability and releasing Zn2+ from cellular stores. TBHQ at 30 µM significantly increased side scatter (cell density) and the exposure of phosphatidylserine (PS) on cell membrane surfaces. It also decreased cellular glutathione (GSH) content without affecting cell lethality. Forward scatter was attenuated by 100 µM TBHQ. Thus, it is considered that TBHQ at sublethal concentrations (30 µM or less) exerts some adverse actions on cells. TBHQ at 10 30 µM attenuated the increase in cell lethality induced by hydrogen peroxide (H2O2), while potentiation of H2O2 cytotoxicity by 100 µM TBHQ was observed. The range of concentrations of TBHQ from benefit to toxicity under in vitro conditions may be 10 30 µM. Although TBHQ exhibits antioxidative actions at concentrations that are lower than those which elicit adverse cellular effects, sublethal levels of TBHQ cause some adverse actions that may be clinically concerned. (Keyword) food additive (Tokushima University Institutional Repository) ● Metadata: 111981 (Link to Publication Site) ● Publication site (DOI): 10.1039/C7TX00183E (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1039/C7TX00183E (Tokushima University Institutional Repository: 111981, DOI: 10.1039/C7TX00183E)
8.	Badr Ali Hoida, Takahashi Keiko, Ryushi Kawakami, Yasuo Oyama, Kumio Yokoigawa and Kaori Kanemaru : Screening and analysis of edible seaweeds in the ability to adsorb Shiga toxin., European Food Research and Technology, Vol.243, No.12, 2147-2153, 2017. (Keyword) Shiga toxin / Seaweed / Ulva linza Linnaeus / Rhamnan sulfate (Tokushima University Institutional Repository) ● Metadata: 111019 (Link to Publication Site) ● Publication site (DOI): 10.1007/s00217-017-2915-1 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1007/s00217-017-2915-1 (Tokushima University Institutional Repository: 111019, DOI: 10.1007/s00217-017-2915-1)
9.	Maki Takeda, Keisuke Oyama, Norio Kamemura, Kaori Kanemaru, Keizo Yuasa, Kumio Yokoigawa and Yasuo Oyama : Change in plasma membrane potential of rat thymocytes by tert-butylhydroquinone, a food additive: Possible risk on lymphocytes, Food and Chemical Toxicology, Vol.109, No.1, 296-301, 2017. (Summary) Tertiary butylhydroquinone (TBHQ) is a food additive and has various beneficial actions under in vitro and in vivo experimental conditions. Therefore, it is necessary to collect additional data on the toxicity of TBHQ in order to avoid adverse effects during clinical applications. Changes in plasma membrane potential are associated with changes in physiological functions even in non-excitable cells such as lymphocytes. Thus, compounds that affect membrane potential may modify some lymphocytic functions. The effect of TBHQ on plasma membrane potential was examined in rat thymocytes using flow cytometric techniques. Treatment of rat thymocytes with TBHQ caused hyperpolarization and then depolarization. The TBHQ-induced hyperpolarization was due to the activation of Ca2+-dependent K+ channels. TBHQ elevated intracellular Ca2+ levels. The depolarization by TBHQ was caused by a nonspecific increase in membrane ionic permeability. Both the sustained depolarization and elevation of intracellular Ca2+ level by TBHQ are thought to be adverse for thymocytes because such changes disturb membrane and intracellular signaling. The thymus is most active during neonatal and pre-adolescent periods. If TBHQ exerts adverse actions on thymocytes, it may result in an immunotoxic effect in neonates and adolescents. (Keyword) tert-butylhydroquinone / food additive / membrane potential / lymphocytes / calcium (Tokushima University Institutional Repository) ● Metadata: 111984 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.fct.2017.09.017 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28899772 ● Summary page in Scopus @ Elsevier: 2-s2.0-85029351916 (Tokushima University Institutional Repository: 111984, DOI: 10.1016/j.fct.2017.09.017, PubMed: 28899772, Elsevier: Scopus)
10.	Goto Tsukie, Makiko Tsuji, Kaori Kanemaru and Kumio Yokoigawa : Adsorption of Shiga Toxin to Poly-γ Glutamate Precipitated., Journal of Food Science, Vol.81, No.12, 2977-2981, 2016. (Tokushima University Institutional Repository) ● Metadata: 111020 (Link to Publication Site) ● Publication site (DOI): 10.1111/1750-3841.13540 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1111/1750-3841.13540 (Tokushima University Institutional Repository: 111020, DOI: 10.1111/1750-3841.13540)
11.	Eiko Niwa, Tsuyoshi Mitani, Shohei Saitoh, Kaori Kanemaru, Shiro Ishida, Kumio Yokoigawa and Yasuo Oyama : Zinc increases vulnerability of rat thymic lymphocytes to arachidonic acid under in vitro conditions, Food and Chemical Toxicology, Vol.96, 177-182, 2016. (Summary) Previous studies on the cytotoxicity of arachidonic acid (ARA) elucidated the involvement of oxidative stress and Ca2+. In the present study, the Zn2+-related cytotoxicity of ARA was studied by a flow cytometric technique with appropriate fluorescent probes in rat thymocytes. Addition of 10 µM ZnCl2 enhanced the increase in cell lethality induced by 10 µM ARA. The removal of Zn2+ by Zn2+ chelators attenuated the ARA-induced increase in cell lethality. Thus, Zn2+ is suggested to be involved in ARA cytotoxicity. ARA at 3 10 µM elevated intracellular Zn2+ level. The Zn2+ chelators attenuated the ARA-induced increase in intracellular Zn2+ level while ARA significantly increased intracellular Zn2+ level in the presence of 3 µM ZnCl2, suggesting the involvement of external Zn2+. Zn2+ reportedly exerts cytotoxic action under oxidative stress induced by hydrogen peroxide, via an excessive increase in intracellular Zn2+ levels. Since ARA induces oxidative stress, the simultaneous administration of zinc and ARA may be harmful. (Keyword) Polyunsaturated fatty acids (Tokushima University Institutional Repository) ● Metadata: 110419 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.fct.2016.08.008 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27515868 ● Search Scopus @ Elsevier (PMID): 27515868 ● Search Scopus @ Elsevier (DOI): 10.1016/j.fct.2016.08.008 (Tokushima University Institutional Repository: 110419, DOI: 10.1016/j.fct.2016.08.008, PubMed: 27515868)
12.	Hiromitsu Tsuzuki, Shota Inoue, Daiki Kobayashi, Gantulga Uuganbaatar, Kaori Kanemaru, Kumio Yokoigawa and Yasuo Oyama : Methyl cinnamate increases cell vulnerability to oxidative stress induced by hydrogen peroxide in rat thymocytes, Fundamental Toxicological Sciences, Vol.3, No.3, 121-125, 2016. (Summary) Methyl cinnamate (MC) and essential oils containing MC possess beneficial antimicrobial, antifungal, and insecticidal effects, among others. Such effects are related to the biocidal action of MC. The antioxidant activity of MC has also been reported elsewhere. It has been suggested that MC may be cytotoxic to cells exposed to oxidative stress. To test this possibility, the effect of MC on rat thymocytes was examined while the cells were subjected to oxidative stress induced by hydrogen peroxide (H2O2). Flow cytometric techniques with appropriate fluorescent probes were used for quantification. MC increased cell vulnerability to oxidative stress via acceleration of the cell death process and/or potentiation of oxidative stress. The use of MC is widespread because of its beneficial actions, and thus further attention should be paid to whether MC is effective under oxidative stress. (Tokushima University Institutional Repository) ● Metadata: 110051 (Link to Publication Site) ● Publication site (DOI): 10.2131/fts.3.121 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.2131/fts.3.121 (Tokushima University Institutional Repository: 110051, DOI: 10.2131/fts.3.121)
13.	Takefumi Hattori, Hiromitsu Tsuzuki, Hiroe Amou, Kumio Yokoigawa and Akira Ohta : A biosynthetic pathway for (E)-methyl cinnamate formation in the ectomycorrhizal fungus Tricholoma matsutake, Mycoscience, Vol.57, No.3, 181-186, 2016. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.myc.2016.01.001 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84958555003 (DOI: 10.1016/j.myc.2016.01.001, Elsevier: Scopus)
14.	Hideshi Teramoto, Yuko Kumeda, Kumio Yokoigawa, Koji Hosomi, Shunji Kozaki, Masafumi Mukamoto and Tomoko Kohda : Genotyping and characterisation of the secretory lipolytic enzymes of Malassezia pachydermatis isolates collected dogs., Veterinary Record Open, Vol.2, No.2, e000124, 2015. (Summary) Malassezia species are commensals of normal skin microbial flora of humans and animals. These may become pathogenic under certain conditions such as those associated with atopic dermatitis or otitis externa in dogs. Isolates of Malassezia pachydermatis were obtained from 27 dogs with healthy external ears and 32 dogs with otitis externa. Isolates were characterised on the basis of their first internal transcribed spacer (ITS) and internal spacer 1 (IGS1) sequences. Their extracellular lipase and phospholipase activity were also analysed. Three types of phospholipase inhibitor were used to identify the subclasses of phospholipase associated with otitis externa. The clinical isolates were classified into three ITS and three IGS1 sequence types. No significant differences in pathogenicity were detected among the ITS or IGS1 genotypes, and all of the isolates exhibited similar levels of lipase activity. The isolates derived from the dogs with otitis externa showed significantly higher phospholipase activity than those obtained from the dogs with healthy external ears. A phospholipase D inhibitor reduced the phospholipase activity of the isolates obtained from the dogs with otitis externa. This study did not show any significant differences in pathogenicity among the ITS or IGS1 genotypes but does suggest that phospholipase D might be one of the virulence factors involved in the inflammation of the external ear caused by M. pachydermatis. (Tokushima University Institutional Repository) ● Metadata: 114976 (Link to Publication Site) ● Publication site (DOI): 10.1136/vetreco-2015-000124 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26392911 ● Search Scopus @ Elsevier (PMID): 26392911 ● Search Scopus @ Elsevier (DOI): 10.1136/vetreco-2015-000124 (Tokushima University Institutional Repository: 114976, DOI: 10.1136/vetreco-2015-000124, PubMed: 26392911)
15.	Takemasa Niro and Kumio Yokoigawa : Analysis of cumin in the ability to suppress verocytotoxin production by enterohemorrhagic Escherichia coli O157., Food Preservation Science, Vol.38, No.4, 217-223, 2012. (Summary) 腸菅出血性大腸菌(EHEC)O157のべロ毒素(VT)産生に対するクミンの抑制能力を調べた．クミン抽出液は70%エチルアルコールを用いて調製した．0．02%クミン抽出液を添加したLB(Luria-Bertani)培地を用いて,37℃で定常期までEHECO157を培養したとき,ベロ毒素生産はVT1とVT2共に有意に低下した．また,この培養条件では,増殖抑制や誘導期の遅延は認められなかった．クミン抽出液の活性成分は,HPLC法によって精製され,4-イソプロピルベンズアルデヒド(IPBA)と同定された．EHEC O157のVT生産に対するIPBAの抑圧効果を調べた結果,細胞内および細胞外のべロ毒素量は,IPBA濃度の上昇とともに低下した．この結果は,IPBAがEHEC O157の有害性を低下させることに有用であることを示唆する． (Keyword) verocytotoxin / enterohemorrhagic Escherichia coli O157 / cumin / spice / 4-isopropylbenzaldehyde (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390010292637661952 (CiNii: 1390010292637661952)
16.	Tsuji Makiko and Kumio Yokoigawa : Attachment of Escherichia coli O157:H7 to abiotic surfaces of cooking utensils., Journal of Food Science, Vol.77, No.4, 194-199, 2012. (Summary) We examined the attachment of enterohemorrhagic Escherichia coli O157:H7 to abiotic surfaces of cooking utensils. When the cell suspension in 0.85% NaCl (about 100 cells/mL, 10 mL) was contacted with various abiotic surfaces (square pieces, 25 cm²) at 25 °C for 20 min, the number of attached cells varied depending on the types of abiotic materials. The pathogen well attached to stainless steel (about 50 cells/25 cm²), pure titanium (35 to 45 cells/25 cm²), and glass (about 20 cells/25 cm²), but little attached to aluminum foil and plastics, irrespective of strains used. Fewer cells (below 10 cells/25 cm²) attached to stainless steel, pure titanium, and glass surfaces conditioned with aseptically sliced beef (sirloin) and autoclaved beef tallow at 25 °C for 20 min, but bovine serum albumin did not reduce the number of attached cells. The cells grown at 15 °C to the stationary phase (OD660 = about 2.8) less attached to the abiotic surfaces than those grown at 25 °C and 37 °C. When we pretreated the cells at 37 °C for 2 h with 50 μM N-hexanoyl-L-homoserine lactone (HHL), the number of cells attached to stainless steel was reduced by 70%. The number of cells attached to cooking utensils seemed to change depending on types of abiotic materials, adhesion of beef tallow to abiotic surfaces, growth temperature of the pathogen, and HHL-producing bacteria. (Keyword) 4-Butyrolactone / Animals / Anti-Bacterial Agents / Bacterial Adhesion / Cattle / Cooking and Eating Utensils / Disinfectants / Escherichia coli O157 / Escherichia coli Proteins / Fats / Gene Expression Regulation, Bacterial / Glass / Meat / Metals / Plastics / RNA, Messenger / Species Specificity / Surface Properties / Temperature (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1750-3841.2012.02654.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22515247 ● Search Scopus @ Elsevier (PMID): 22515247 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1750-3841.2012.02654.x (DOI: 10.1111/j.1750-3841.2012.02654.x, PubMed: 22515247)
17.	Makiko Tsuji and Kumio Yokoigawa : Acid resistance and verocytotoxin productivity of enterohemorrhagic Escherichia coli O157:H7 exposed to microwave, Journal of Food Science, Vol.76, No.6, 445-449, 2011. (Summary) We examined the acid resistance and verocytotoxin (VT) productivity of enterohemorrhagic Escherichia coli O157:H7 irradiated by microwave with a domestic microwave oven and a commercial microwave radiator equipped with a thermo-regulator. When the cell suspension (5 mL) chilled at 0 °C was treated with a domestic microwave oven at weak power (2.45 GHz, 100 W) for 60 s, the living cell number was reduced by 2 orders (final temperature, about 65 °C). The surviving cells showed lower acid resistance and VT productivity than nonirradiated cells. To examine the nonthermal effect of microwave on acid resistance and VT productivity, the cells in Luria-Bertani medium were intermittently irradiated to keep the culture temperature at 37 °C with the microwave radiator (2.45 GHz, 0.6 W/mL). The intermittent radiation slightly reduced the acid resistance, but clearly suppressed the VT productivity. Microwave oven is probably useful for reducing not only the living cell number but also the acid resistance and VT productivity of EHEC O157:H7. (Keyword) Colony Count, Microbial / Escherichia coli Infections / Escherichia coli O157 / Feces / Food Irradiation / Foodborne Diseases / Hot Temperature / Humans / Hydrogen-Ion Concentration / Japan / Microbial Viability / Microwaves / Shiga Toxin 1 / Shiga Toxin 2 / Shiga Toxins / Species Specificity / Time Factors / Virulence (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1750-3841.2011.02225.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22417515 ● Search Scopus @ Elsevier (PMID): 22417515 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1750-3841.2011.02225.x (DOI: 10.1111/j.1750-3841.2011.02225.x, PubMed: 22417515)
18.	Kuniko Nagasaka, Hideyuki Nakagawa, Fumihiko Satoh, Takefumi Hosotani, Kumio Yokoigawa, Hitomi Sakai, Haruhiko Sakuraba, Toshihisa Ohshima, Mitsuko Shinohara and Kiyoshi Ohura : A novel cytotoxic protein, Karatoxin, from the dorsal spines of the the redfin velvetfihi, Hypodytes rubripinnis, Toxin Reviews, Vol.28, No.4, 260-265, 2009. (Keyword) Hypodytes rubripinnis / Karatoxin (Link to Publication Site) ● Publication site (DOI): 10.3109/15569540903348940 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-72049118862 (DOI: 10.3109/15569540903348940, Elsevier: Scopus)
19.	Niro Takemasa, Shinya Ohnishi, Makiko Tsuji, Tomoko Shikata and Kumio Yokoigawa : Screening and Analysis of Spices with Ability to Suppress Verocytotoxin Production by Escherichia coli O157, Journal of Food Science, Vol.74, No.8, 461-466, 2009. (Summary) To reduce the amounts of verocytotoxin (VT) produced by Escherichia coli O157:H7, various spices were screened for their ability to suppress VT production. Extracts of these spices were prepared with 70% ethyl alcohol. When E. coli O157:H7 cells were grown to the stationary phase at 37 degrees C in Luria-Bertani medium supplemented with 0.02% allspice extract, the production of both VT1 and VT2 was significantly reduced. Neither growth inhibition nor a delay in the lag phase was observed when the cells were cultured in the presence of 0.02% allspice extract. An active component of the allspice extract was purified by HPLC and was identified as eugenol. When we examined the suppressive effect of eugenol on VT production by E. coli O157:H7, the amounts of both intracellular and extracellular VTs were found to decrease with an increase in eugenol concentration. Our results suggest that eugenol is useful for reducing the virulence of E. coli O157:H7. (Keyword) Anti-Infective Agents / Colony Count, Microbial / Dose-Response Relationship, Drug / Drug Evaluation, Preclinical / Escherichia coli O157 / Eugenol / Feces / Food Additives / Humans / Latex Fixation Tests / Pimenta / Plant Extracts / Shiga Toxin 1 / Shiga Toxin 2 / Shiga Toxins / Spices (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1750-3841.2009.01326.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19799674 ● CiNii @ National Institute of Informatics (CRID): 1573105976314398720 ● Search Scopus @ Elsevier (PMID): 19799674 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1750-3841.2009.01326.x (DOI: 10.1111/j.1750-3841.2009.01326.x, PubMed: 19799674, CiNii: 1573105976314398720)
20.	Machiko Sato, Kyoko Kanie, Kenji Soda and Kumio Yokoigawa : Suppressive Effect of Poly-γ-glutamate on SOS Response of Salmonella typhimurium Induced by Chemical Mutagens, Journal of Bioscience and Bioengineering, Vol.105, No.6, 690-693, 2008. (Summary) 納豆の主要粘性物質であるポリγ-グルタミン酸(PGA)の抗変異原性を検討した．PGAは食品添加物として使用されている酸性多糖類よりも高い抗変異原性を示した．またPGAの分子量によって，抗変異原活性が異なることを明らかにした． (Keyword) Dose-Response Relationship, Drug / Drug Interactions / Mutagens / Particle Size / Polyglutamic Acid / SOS Response (Genetics) / Salmonella typhimurium (Link to Publication Site) ● Publication site (DOI): 10.1263/jbb.105.690 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18640614 ● Search Scopus @ Elsevier (PMID): 18640614 ● Search Scopus @ Elsevier (DOI): 10.1263/jbb.105.690 (DOI: 10.1263/jbb.105.690, PubMed: 18640614)
21.	Kumio Yokoigawa, Machiko Sato and Kenji Soda : Simple improvement in freeze-tolerance of bakers' yeast with poly-γ-glutamate, Journal of Bioscience and Bioengineering, Vol.102, No.3, 215-219, 2006. (Summary) 納豆の主要粘性物質であるポリγ-グルタミン酸は無味，無臭であり，高い保水性と陽イオン結合性等の特徴を有することから，食品分野での利用が期待されている．本研究では，ポリγ-グルタミン酸を用いて，パン酵母の冷凍耐性を簡便に改善する方法を確立した． (Keyword) Cold Temperature / Cryopreservation / Food Microbiology / Polyglutamic Acid / Saccharomyces cerevisiae / Time Factors (Link to Publication Site) ● Publication site (DOI): 10.1263/jbb.102.215 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17046536 ● Search Scopus @ Elsevier (PMID): 17046536 ● Search Scopus @ Elsevier (DOI): 10.1263/jbb.102.215 (DOI: 10.1263/jbb.102.215, PubMed: 17046536)
22.	Jiansong Ju, Kumio Yokoigawa, Haruo Misono and Kouhei Ohnishi : Cloning of alanine racemase genes from Pseudomonas fluorescens strains and oligomerization states of the gene products expressed in Escherichia coli., Journal of Bioscience and Bioengineering, Vol.100, No.4, 409-417, 2005. (Summary) 細菌細胞壁の生合成に必須の役割を果たすアラニンラセマーゼの酵素化学的特徴を明らかにするため，Pseudomonas fluorescens由来の本酵素遺伝子をクローニングして大腸菌細胞内で発現させた．生成した酵素は，サブユニット間の相互作用により，単量体と二量体の動的平衡状態であることが明らかとなった． (Keyword) Alanine Racemase / Catalysis / Cloning, Molecular / Dimerization / Escherichia coli / Gene Order / Genes, Bacterial / Mutation / Phylogeny / Protein Subunits / Pseudomonas fluorescens (Link to Publication Site) ● Publication site (DOI): 10.1263/jbb.100.409 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16310730 ● Search Scopus @ Elsevier (PMID): 16310730 ● Search Scopus @ Elsevier (DOI): 10.1263/jbb.100.409 (DOI: 10.1263/jbb.100.409, PubMed: 16310730)
23.	Shingo Izawa, Machiko Sato, Kumio Yokoigawa and Yoshiharu Inoue : Intracellular glycerol influences resistance to freeze stress in Saccharomyces cerevisiae: analysis of a quadruple mutant in glycerol-dehydrogenase genes and glycerol-enriched cells., Applied Microbiology and Biotechnology, Vol.66, No.1, 108-114, 2004. (Summary) 酵母の冷凍耐性における細胞内グリセロールの役割を解明するために，グリセロール代謝にかかわる4つの遺伝子を破壊した変異株を作製した．変異株の細胞内グリセロール含量は野生株よりも上昇し，冷凍耐性も増加した．以上のことから，酵母の冷凍耐性にグリセロールは重要な役割を果たすことが明らかとなった． (Keyword) Fermentation / Food Technology / Freezing / Glycerol / Mutation / Saccharomyces cerevisiae / Sugar Alcohol Dehydrogenases / Time Factors (Link to Publication Site) ● Publication site (DOI): 10.1007/s00253-004-1624-4 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15127164 ● CiNii @ National Institute of Informatics (CRID): 1572824501333965184 ● Search Scopus @ Elsevier (PMID): 15127164 ● Search Scopus @ Elsevier (DOI): 10.1007/s00253-004-1624-4 (DOI: 10.1007/s00253-004-1624-4, PubMed: 15127164, CiNii: 1572824501333965184)
24.	Kumio Yokoigawa, Yoko Okubo, Kenji Soda and Haruo Misono : Improvement in Thermostability and Psychrophilicity of Psychrophilic Alanine Racemase by Site-directed Mutagenesis., Journal of Molecular Catalysis B: Enzymatic, Vol.23, No.2-6, 389-395, 2003. (Summary) これまでの低温性アラニンラセマーゼに関する構造と機能に関する研究で得られた知見に基づき，本酵素に部位特異的変異を導入した．得られた変異酵素は，低温での触媒活性と熱安定性が共に向上したことから，耐熱性と低温性は表裏一体の性質ではないことが判明した． (Link to Publication Site) ● Publication site (DOI): 10.1016/S1381-1177(03)00103-6 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/S1381-1177(03)00103-6 (DOI: 10.1016/S1381-1177(03)00103-6)
25.	Naomi Mizobuchi, Kumio Yokoigawa, Terue Harumoto, Hiromi Fujisawa and Yoshiomi Takagi : Catalase is the Bacteria-derived Detoxifying Substance against Paramecium-killing Toxin in Wheat Grass Powder Infusion., The Journal of Eukaryotic Microbiology, Vol.50, No.4, 299-303, 2003. (Summary) 小麦若葉粉末の抽出液に含まれるゾウリムシに対する毒物質を無毒化できる細菌由来タンパク質はカタラーゼであることを証明し，かつ，毒物質は微量(約30 μM)の過酸化水素であることを解明した．ゾウリムシ由来カタラーゼは細胞内で生成する過酸化水素には作用するが細胞外の過酸化水素は無毒化できないことが判明した． (Keyword) Amino Acid Sequence / Animals / Catalase / Culture Media / Hydrogen Peroxide / Klebsiella pneumoniae / Molecular Weight / Paramecium / Peroxidase (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1550-7408.2003.tb00138.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15132174 ● Search Scopus @ Elsevier (PMID): 15132174 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1550-7408.2003.tb00138.x (DOI: 10.1111/j.1550-7408.2003.tb00138.x, PubMed: 15132174)
26.	Kumio Yokoigawa, Akiko Takikawa, Yoko Okubo and Seiichi Umesako : Acid Tolerance and gad mRNA Levels of Escherichia coli O157:H7 Grown in Foods., International Journal of Food Microbiology, Vol.82, No.3, 203-211, 2003. (Summary) 各種食品中で増殖した大腸菌O157の酸耐性の変動とグルタミン酸脱炭酸酵素の転写レベルの変動を明らかにし，大腸菌O157の酸耐性にはグルタミン酸脱炭酸酵素の活性が深く関与することを明らかにした． (Keyword) Adaptation, Physiological / Animals / Escherichia coli / Escherichia coli O157 / Escherichia coli Proteins / Food Microbiology / Glutamate Decarboxylase / Hydrogen-Ion Concentration / Meat / Membrane Proteins / Milk / RNA, Messenger / Temperature / Vegetables (Link to Publication Site) ● Publication site (DOI): 10.1016/S0168-1605(02)00305-7 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12593923 ● Search Scopus @ Elsevier (PMID): 12593923 ● Search Scopus @ Elsevier (DOI): 10.1016/S0168-1605(02)00305-7 (DOI: 10.1016/S0168-1605(02)00305-7, PubMed: 12593923)
27.	Kumio Yokoigawa, Yoko Okubo and Kenji Soda : Subunit Interaction of Monomeric Alanine Racemases from Four Shigella Species in Catalytic Reaction., FEMS Microbiology Letters, Vol.221, No.2, 263-267, 2003. (Summary) 赤痢菌由来アラニンラセマーゼは，単量体と二量体の動的平衡状態にあることを見いだし，活性発現に二量体形成が必須であることから，サブユニット相互作用を阻害する新しいタイプの酵素阻害剤が開発可能であることを示した． (Keyword) Alanine Racemase / Catalysis / Catalytic Domain / Cycloserine / Enzyme Inhibitors / Kinetics / Shigella boydii / Shigella dysenteriae / Shigella flexneri / Shigella sonnei / Stereoisomerism (Link to Publication Site) ● Publication site (DOI): 10.1016/S0378-1097(03)00216-7 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12725937 ● Search Scopus @ Elsevier (PMID): 12725937 ● Search Scopus @ Elsevier (DOI): 10.1016/S0378-1097(03)00216-7 (DOI: 10.1016/S0378-1097(03)00216-7, PubMed: 12725937)
28.	Akiko Takikawa, Keiko Abe, Makiko Yamamoto, Shoko Ishimaru, Mari Yasui, Yoko Okubo and Kumio Yokoigawa : Antimicrobial Activity of Nutmeg against Escherichia coli O157., Journal of Bioscience and Bioengineering, Vol.94, No.4, 315-320, 2002. (Summary) 各種香辛料の大腸菌O157に対する抗菌活性を，非病原性大腸菌との比較において検討し，ナツメグが大腸菌O157の特異的殺菌に有効であることを見い出した．抗菌成分の精製も行い，β-ピネンが大腸菌O157の殺菌に有効であることを明らかにした． (Link to Publication Site) ● Publication site (DOI): 10.1016/S1389-1723(02)80170-0 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16233309 ● Summary page in Scopus @ Elsevier: 2-s2.0-0036429670 (DOI: 10.1016/S1389-1723(02)80170-0, PubMed: 16233309, Elsevier: Scopus)
29.	Mineko Iwahori, Kaneko Akamatsu, Sachie Kurokawa, Kumio Yokoigawa, Hiroshi Ueno, Hiroshi Ogawa, Hitoshi Ozawa and Mitsuhiro Kawata : Immunohistochemical study of the localization of glutamate decarboxylase in rodent's submandibular gland, Journal of Biological Macromolecules, Vol.2, No.0, 76-77, 2002. (Summary) グルタミン酸脱炭酸酵素が顎下腺に局在していることを示し，唾液のpH調整に機能している可能性を示唆した． (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1570854176752570880 (CiNii: 1570854176752570880)
30.	Kumio Yokoigawa, Reiko Hirasawa, Hiroshi Ueno, Yoko OKUBO, Seiichi Umesako and Kenji Soda : Gene Cloning and Characterization of Alanine Racemases from Shigella dysenteriae, Shigella boydii, Shigella flexneri, and Shigella sonnei., Biochemical and Biophysical Research Communications, Vol.288, No.3, 676-684, 2001. (Summary) Alanine racemase genes (alr) from Shigella dysenteriae, Shigella boydii, Shigella flexneri, and Shigella sonnei were cloned and expressed in Escherichia coli JM109. All genes encoded a polypeptide of 359 amino acids, and showed more than 99% sequence identities with each other. In particular, the S. dysenteriae alr was identical with the S. flexneri alr. Differences in the amino acid sequences between the four Shigella enzymes were only two residues: Gly138 in S. dysenteriae and S. flexneri (Glu138 in the other) and Ile225 in S. sonnei (Thr225 in the other). The S. boydii enzyme was identical with the E. coli K12 alr enzyme. Each Shigella alr enzyme purified to homogeneity has an apparent molecular mass about 43,000 by SDS-gel electrophoresis, and about 46,000 by gel filtration. However, all enzymes showed an apparent molecular mass about 60,000 by gel filtration in the presence of a substrate, 0.1 M l-alanine. These results suggest that the Shigella alr enzymes having an ordinary monomeric structure interact with other monomer in the presence of the substrate. The enzymes were almost identical in the enzymological properties, and showed lower catalytic activities (about 210 units/mg) than those of homodimeric alanine racemases reported. (Keyword) Alanine Racemase / Amino Acid Sequence / Base Sequence / Cloning, Molecular / DNA, Bacterial / Kinetics / Molecular Sequence Data / Molecular Weight / Polymerase Chain Reaction / Sequence Homology, Amino Acid / Shigella boydii / Shigella dysenteriae / Shigella sonnei / Temperature (Link to Publication Site) ● Publication site (DOI): 10.1006/bbrc.2001.5817 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11676496 ● Summary page in Scopus @ Elsevier: 2-s2.0-0035798125 (DOI: 10.1006/bbrc.2001.5817, PubMed: 11676496, Elsevier: Scopus)
31.	Reiko Hirasawa, Kumio Yokoigawa, Yuka Isobe and Hiroyasu Kawai : Improving the Freeze-tolerance of Bakers' Yeast by Loading with Trehalose., Bioscience, Biotechnology, and Biochemistry, Vol.65, No.3, 522-526, 2001. (Summary) We examined the freeze tolerance of bakers' yeast loaded with exogenous trehalose. Freeze-tolerant and freeze-sensitive compressed bakers' yeast samples were soaked at several temperatures in 0.5 M and 1 M trehalose and analyzed. The intracellular trehalose contents in both types of bakers' yeast increased with increasing soaking period. The initial trehalose-accumulation rate increased with increasing exogenous trehalose concentration and soaking temperature. The maximum trehalose content was almost identical (200-250 mg/g of dry cells) irrespective of the soaking temperature and the type of bakers' yeast, but depended on the exogenous trehalose concentration. The leavening ability of both types of bakers' yeast loaded with trehalose was almost identical to that of the respective original cells, irrespective of the soaking conditions. The freeze-tolerant ratio (FTR) of both types of bakers' yeast increased with increasing intracellular trehalose content. However, FTR decreased during over-soaking after the maximum amount of trehalose had accumulated. FTR of the freeze-sensitive bakers' yeast was more efficiently improved than that of the freeze-tolerant type. (Keyword) Freezing / Saccharomyces cerevisiae / Trehalose (Link to Publication Site) ● Publication site (DOI): 10.1271/bbb.65.522 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11330663 ● Summary page in Scopus @ Elsevier: 2-s2.0-0035291332 (DOI: 10.1271/bbb.65.522, PubMed: 11330663, Elsevier: Scopus)
32.	Kumio Yokoigawa, Yoko Okubo, Hiroyasu Kawai, Nobuyoshi Esaki and Kenji Soda : Structure and function of Psychrophilic Alanine Racemase., Journal of Molecular Catalysis B: Enzymatic, Vol.12, No.1-6, 27-35, 2001. (Summary) 低温性アラニンラセマーゼの低温での高い触媒活性と低い耐熱性に関与するアミノ酸残基を特定し，低温性酵素の構造と機能の関係を明らかにした． (Link to Publication Site) ● Publication site (DOI): 10.1016/S1381-1177(00)00200-9 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0035961610 (DOI: 10.1016/S1381-1177(00)00200-9, Elsevier: Scopus)
33.	Yuka ISOBE, Tomoyo KATAMOTO, Yuki KAWAGUCHI, Sachiko MATSUSHITA, Kumio Yokoigawa, Toko AKIRA and Hiroyasu KAWAI : Suppressive Effects of the Extract from Sake Cake on Chemical Mutagen-Induced SOS Response in Salmonella typhimurium., Food Science and Technology Research, Vol.7, No.1, 64-66, 2001. (Summary) The suppressive effects of the extract from sake cake on the SOS response of Salmonella typhimurium TA 1535/pSK1002 induced by AF-2, 4NQO, Trp-P-1, Trp-P-2, IQ and MeIQx were investigated for the purpose of finding antimutagenic substances in sake cake. The water-extract and methanol-extract from sake cake strongly suppressed SOS response induced by IQ in the presence of S9 mix in a dose dependent manner. The molecular weight of a suppressive compound in the water-extract was less than 3000 in an analysis of gel filtration. Several suppressive compounds also seemed to exist in the methanol-extract from sake cake. (Keyword) sake cake / SOS response / suppression / antimutagenicity (Link to Publication Site) ● Publication site (DOI): 10.3136/fstr.7.64 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282679433276544 ● Summary page in Scopus @ Elsevier: 2-s2.0-0347608797 (DOI: 10.3136/fstr.7.64, CiNii: 1390282679433276544, Elsevier: Scopus)
34.	Kenji Soda, Tadao Oikawa and Kumio Yokoigawa : One-pot chemo-enzymatic enantiomerization of racemates., Journal of Molecular Catalysis B: Enzymatic, Vol.11, No.4-6, 149-153, 2001. (Summary) DL-アミノ酸およびDL-乳酸を，それぞれ1つの反応器内で酸化反応と還元反応を効率的に進めることにより，高収率で光学分割できることを示した． (Link to Publication Site) ● Publication site (DOI): 10.1016/S1381-1177(00)00181-8 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0035931377 (DOI: 10.1016/S1381-1177(00)00181-8, Elsevier: Scopus)
35.	Reiko Hirasawa and Kumio Yokoigawa : Leavening Ability of Bakers' Yeast Exposed to Hyperosmotic Media., FEMS Microbiol. Lett., Vol.194, 159-162, 2001. (Summary) 高浸透圧を負荷したパン酵母は，高い発酵力を有することを見い出し，その生理学的変化を細胞内物質の消長から解析して，発酵力上昇の機構を解明した．
36.	Sachie Kurohara, Maiko Asai, Miyuki Hayashi, Kumio Yokoigawa and Hiroshi Ueno : Microanalysis of GABA: an application for evaluating GABA production in yeast strains and the effect of spice extracts on glutamate decarboxylase activity., Journal of Biological Macromolecules, Vol.1, 45-48, 2001. (Summary) 機能性物質として有用なガンマーアミノ酪酸の種々の酵母における含量を測定すると共に，細胞内ガンマーアミノ酪酸含量に対するスパイスの影響を明らかにした．
37.	A. Teramoto, Y. Kusao, N. Bagum, Y. Isobe, Kumio Yokoigawa and H. Kawai : Improvable effect of sodium chloride on leavening activity of Sakadane., Res. J. Living Sci., Vol.46, 45-50, 2000. (Summary) 酒種の発酵力に対する浸透圧の影響を検討し，1ー3%塩化ナトリウム存在下で培養した酒種は，高い発酵力を有することを明らかにした．
38.	Yoko Okubo, Kumio Yokoigawa, Nobuyoshi Esaki, Kenji Soda and Haruo Misono : High Catalytic Activity of Alanine Racemase from Psychrophilic Bacillus psychrosaccharolyticus at High Temperatures in the Presence of Pyridoxal 5'-phosphate., FEMS Microbiol. Lett., Vol.192, 169-173, 2000. (Summary) 補酵素の存在下でBacilus psychrosaccharolyticus 由来の低温性アラニンラセマーゼは好熱菌由来酵素より高い触媒活性を有することを明らかにした．低温で高い触媒活性を有する低温性酵素は，触媒として高い潜在能力を有することを示す最初の論文である．
39.	Kumio Yokoigawa, Takigawa Akiko and Kawai Hiroyasu : Difference between Escherichia coli O157:H7 and Non-pathogenic E. coli: Survival and Growth in Seasonings., Journal of Bioscience and Bioengineering, Vol.88, No.5, 574-576, 1999. (Summary) We examined the survival and growth of Escherichia coli O157:H7 cells incubated with several seasonings, in comparison with those of non-pathogenic E.coli. The cells were incubated at 25℃ for 24 h with several concentrations of NaCl, sucrose, soy sause, worcester sause and tomato ketchup, and their survival ratios were determined. The E.coli O157:H7 strains showed relatively higher survival ratios in 0.5-1.0 M sucrose, 25% soy sauce and 12.5-50% worcester sauce than the non-pathogenic strains, but slightly lower survival ratios in 0.5-2.0 M NaCl. A noteworthy difference between E.coli O157:H7 and the non-pathogenic strains was that incubation in the presence of 12.5% soy sauce allowed the growth of E.coli O157:H7 strains but reduced the viable cell numbers of non-pathogenic E.coli strains. (Keyword) Escherichia coli O157 / survival / seasonings (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1521699230329029248 (CiNii: 1521699230329029248)
40.	Bagum Nazneen, Kumio Yokoigawa, Teramoto Ai, Isobe Yuka and Kawai Hiroyasu : Effect of Salt Stress on the Leavening Ability of Bakers' Yeast., Journal of Home Economics of Japan, Vol.50, No.7, 729-733, 1999. (Summary) The leavening ability of bakers' yeast cells grown in several media containing NaCl was evaluated in several types of dough. Three types of growth media were used: YPG medium containing 5% glucose, 1% peptone, 0.5% yeast extract, 0.1% KH_2PO_4 and 0.1% MgSo_4·7H_2O (pH 5.6); YPS medium containing 2% sucrose, 4% peptone, 2% yeast extract, 0.2% KH_2PO_4 and 0.1% MgSO_4·7H_2O (pH 5.6); and MO medium containing 1% urea, 0.2% KH_2PO_4, and 5% total sugar as cane molasses (pH 5.6). The addition of 3% NaCl to each of these growth media clearly suppressed the growth of the yeast cells, irrespective of the types of media; the cell yield was decreased by 15-60% in the stationary phase. When we analyzed the leavening ability of these cells that had been exposed to salt stress, the cells showed higher leavening ability than those grown in the absence of NaCl, irrespective of the types of growth media and dough. The increase ratio of leavening ability in the presence of NaCl was highest at 45% when we used the YPS of YPG medium containing 3% NaCl and dough supplemented with 5% glucose. However, the increase was at a much lower level (2-7%) in sugar-less dough, irrespective of the types of growth media. Since the leavening ability of yeast is well known to be primarily limited by the rate of sugar uptake, the high leavening ability of bakers' yeast grown in the presence of NaCl may be due to an increase in sugar-uptake rate. The presence of NaCl in each growth medium also enhanced the leavening ability of various Saccharomyces yeast strains. (Received July 8, 1998; Accepted in revised form February 17, 1999) (Keyword) bakers' yeast / leavening ability / salt stress (Link to Publication Site) ● Publication site (DOI): 10.11428/jhej1987.50.729 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282681309918464 ● Search Scopus @ Elsevier (DOI): 10.11428/jhej1987.50.729 (DOI: 10.11428/jhej1987.50.729, CiNii: 1390282681309918464)
41.	Kumio Yokoigawa, Kaori Inoue, Yoko Okubo and Hiroyasu KAWAI : Primers for Amplifying an Alanine Racemase Gene Fragment to Detect E. coli strains in Food., Journal of Food Science, Vol.64, No.4, 571-575, 1999. (Summary) ポリメラーゼ連鎖反応(PCR)法により，種々の食品中の大腸菌を迅速かつ特異的に検出する方法を検討した．大腸菌アラニンラセマーゼ遺伝子の配列における大腸菌に特異的な領域を検索し，その領域に対するオリゴヌクレオチドプライマーを用いるPCR法を検討したところ，種々の食品中の大腸菌を5時間以内に特異的に検出できることが明らかとなった． (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1365-2621.1999.tb15087.x (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0032842005 (DOI: 10.1111/j.1365-2621.1999.tb15087.x, Elsevier: Scopus)
42.	Yoko Okubo, Kumio Yokoigawa, Nobuyoshi Esaki, Kenji Soda and Hiroyasu Kawai : Characterization of Psychrophilic Alanine Racemase from Bacillus psychrosaccharolyticus,, Biochemical and Biophysical Research Communications, Vol.256, No.2, 333-340, 1999. (Summary) 低温細菌 Bacilus psychrosaccharolyticus 由来の低温性アラニンラセマーゼ遺伝子をクローニングし，その塩基配列を決定すると共に，クローン株から本酵素を均一に精製し，低温性酵素の特性を明らかにした．また，本酵素のアミノ酸配列から，高次構造を予測し，構造と機能の関係を考察した．
43.	Yoko Okubo, Kumio Yokoigawa and Hiroyasu Kawai : Effect of Ethyl Alcohol on Growth and Intracellular Alanine Racemase of Psychrotrophs., Journal of Bioscience and Bioengineering, Vol.87, No.2, 241-244, 1999. (Summary) The psychrotrophic alanine racemase from Pseudomonas fluorescens, a typical psychrotroph, is less resistant to organic solvents than the enzymes from thermophilic and mesophilic bacteria (Okubo et al., J. Home Econ. Jpn., 46: 1135-1140, 1995). To further elucidate this difference, we examined the effect of ethyl alcohol on the growth and intracellular alanine racemase activity of three typical psychrotrophs-P. fluorescens, Bacillus psychrosaccharolyticus and B. psychrophilus-in comparison with two mesophiles, Escherichia coli and B. subtilis. Although all the bacteria grew to the early stationary phase when cultivated at 22 degrees C for 36 h in the absence of ethyl alcohol, the growth of the psychrotrophs was more effectively suppressed by the addition of 3 and 5% ethyl alcohol to the medium than that of the mesophiles. The intracellular alanine racemase activity of the psychrotrophs was also more markedly reduced in the presence of ethyl alcohol than that of the mesophiles. When bacterial cells of each strain grown at 22 degrees C for 36 h in the absence of alcohol were suspended in 0-5 % ethyl alcohol solution and incubated at 30 degrees C for 1 h, both the survival ratio and intracellular alanine racemase activity of the psychrotrophs were lower than those of the mesophiles. Thus, ethyl alcohol effectively reduced both the growth of the psychrotrophs and their intracellular alanine racemase activity. Low concentrations of various other alcohols also repressed the growth of the psychrotrophs at 10 degrees C. (Link to Publication Site) ● Publication site (DOI): 10.1016/S1389-1723(99)89021-5 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16232459 ● Search Scopus @ Elsevier (PMID): 16232459 ● Search Scopus @ Elsevier (DOI): 10.1016/S1389-1723(99)89021-5 (DOI: 10.1016/S1389-1723(99)89021-5, PubMed: 16232459)
44.	Kawai Hiroyasu, Bagum Nazneen, Teramoto Ai, Isobe Yuka and Kumio Yokoigawa : Effect of Sodium Chloride on Growth and Fermentative Activity of Saccharomyces Yeast,, Res. J. Liv. Sci., Vol.45, 55-61, 1999. (Summary) 塩化ナトリウム存在下で生育したパン酵母の発酵力を検討した．2ー3%の塩化ナトリウム存在下で生育したパン酵母は，高い発酵力を示し，この効果は種々のサッカロミセス酵母においても見られた．
45.	Kumio Yokoigawa, Yoko Okubo and Hiroyasu Kawai : A New Sterility Test for Cow's Milk Using Alanine Racemase Gene as the Index., Journal of Bioscience and Bioengineering, Vol.88, No.1, 26-29, 1999. (Summary) Oligonucleotide primers designed from consensus sequences of alanine racemase genes were used for a sterility test of cow's milk by polymerase chain reaction (PCR). Commercial cow's milk in two 250 ml packages was separately centrifuged at 5000 x g for 10 min, bacterial cells in each precipitate were cultivated at 30 and 55 degrees C for 5 h in Luria-Bertani medium, and the cells from each culture were mixed and used for the PCR after being treated with 0.1 N NaOH at 60 degrees C for 10 min. When we performed the PCR using DNAs from various bacteria and eukaryotes as the templates, a unique PCR product of about 390 bp was amplified only from the bacteria. The sensitivity of the PCR method was such that an initial inoculum of 1 CFU of Bacillus stearothermophilus, Escherichia coli, and Pseudomonas fluorescens per 250 ml of cow's milk could be detected. When we analyzed 14 types of commercial cow's milk, all samples which were positive by the standard sterility test at 30 or 55 degrees C were also found positive by the PCR method. (Link to Publication Site) ● Publication site (DOI): 10.1016/S1389-1723(99)80170-4 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16232568 ● Search Scopus @ Elsevier (PMID): 16232568 ● Search Scopus @ Elsevier (DOI): 10.1016/S1389-1723(99)80170-4 (DOI: 10.1016/S1389-1723(99)80170-4, PubMed: 16232568)
46.	Yoko Okubo, Kumio Yokoigawa and Hiroyasu Kawai : Alanine Racemase Gene Fragments as Probes for Detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in Foods., Journal of fermentation and bioengineering, Vol.85, No.6, 559-563, 1998. (Summary) Alanine recemase gene fragments containing non-conserved regions of the gene were evaluated as probes for detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in foods. The gene fragments were amplified from genomic DNA of each bacterium by polymerase chain reaction with degenerate oligonucleotide primers, and labeled with digoxigenin as probes for detecting each bacterium. Foods and bacteria were treated at 25℃ for 10 min in 0.1 N NaOH containing 0.5% SDS before being directly spotted onto nylon membranes for DNA hybridization. When the specificities of the probes were analyzed using a total of 86 strains(23 genera and 65 species)of bacteria including 29 Bacillus strains(20 species), each probe was specific for the respective target bacteria. A variety of foods inoculated with B. stearothermophilus or B. psychrosaccharolyticus were positive as determined by hybridization with the respective probe, whereas uninoculated foods were negative. The alanine recemase gene fragments could be used as specific probes for detecting B. stearothermophilus and B. psychrosaccharolyticus in foods. (Keyword) alanine racemase / Bacillus stearothermophilus / Bacillus psychrosaccharolyticus / detection (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1522825129944697344 (CiNii: 1522825129944697344)
47.	Junko DOI, Kumio Yokoigawa, Yuka Isobe and Hiroyasu KAWAI : Trehalose 6-phosphate Production with Energy Coupling Fermentation by Yeast Cells., Bioscience, Biotechnology, and Biochemistry, Vol.62, No.4, 735-739, 1998. (Summary) パン酵母のエネルギー共役発酵によってトレハロース-6-リン酸(T6P)を生産する方法を検討した．種々の条件検討によって，対糖収率11%でT6Pが生産された．
48.	ISOBE Yuka, Kumio Yokoigawa and Kawai Hiroyasu : Suppressing Effects of Polysaccharide Produced by Bacillus circulans on Chemical Mutagens-Induced SOS Responce in Salmonella typhimurium., Food Sci. Technol. Int. Tokyo, Vol.4, 77-79, 1998. (Summary) Salmonella typhimurium における化学的変異原誘導性のSOS反応に対する，Bacillus circulans 由来多糖の抑制効果を検討した．その結果，本多糖は，IQ，MelQxによって誘導されるSOS反応を強く抑制し，抗変異原性を有することが明らかとなり，食品添加物としての有用性が示唆された．
49.	Bagum Nazneen, Kumio Yokoigawa, Isobe Yuka and KAWAI Hiroyasu : Trehalose Metabolism and Leavening Ability of Bakers' Yeast Grown in the Presence of Sodium Chloride., Journal of Fermentation and Bioengineering, Vol.86, No.5, 457-460, 1998. (Summary) 塩化ナトリウム存在下で生育したパン酵母の細胞内トレハロースは，一時的に増加しても蓄積されず，浸透圧ストレスを受けた酵母の高い発酵力は，トレハロース含量と相関しないことが明らかとなった． (Link to Publication Site) ● Publication site (DOI): 10.1016/S0922-338X(98)80151-7 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0032445190 (DOI: 10.1016/S0922-338X(98)80151-7, Elsevier: Scopus)
50.	Kumio Yokoigawa, Yoko Murakami, Yuka Isobe and Hiroyasu KAWAI : Electrophoretic Karyotypes of Commercial Freeze-tolerant and Freeze-sensitive Bakers' Yeast., Journal of Home Economics of Japan, Vol.48, No.3, 231-234, 1997. (Summary) 市販の冷凍耐性パン酵母と冷凍感受性パン酵母の電気泳動核型をパルスフィールドゲル電気泳動により分析した．パン酵母には16本の染色体DNAが存在するが，冷凍耐性酵母と冷凍感受性酵母の電気泳動核型において，約500kpbのサイズを有する染色体は，冷凍感受性株にのみ認められた．このことは，冷凍耐性株の同定や汚染感受性酵母の検出において，迅速分析を可能にするものと考えられる． (Keyword) bakers' yeast / freeze-tolerant / karyotype (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520853833307369728 (CiNii: 1520853833307369728)
51.	Yuka ISOBE, Kumio Yokoigawa, Hiroyasu Kawai and Yoshiaki Sone : Structural Study of an Exocellular Polysaccharide of Bacillus circulans., Bioscience, Biotechnology, and Biochemistry, Vol.61, 520-524, 1997. (Summary) Bacillus circulans の生産する高粘性多糖を均一に精製し，その構造解析を行った．本多糖は，ガラクトース，マンノース，ラムノース，グルクロン酸を含むオリゴ糖の繰り返し構造を有することが明らかとなった．
52.	Kumio Yokoigawa, Okubo Yoko, Nakahira Mayumi, Umesako Seiichi, Imai Shunsuke and Kawai Hiroyasu : Evaluation of an Alanine Racemase Gene as an Indicator for the Detection of Various Escherichia coli: Reactivity of the Gene Fragment with Various E. coli O157:H7 Isolates., Journal of Fermentation and Bioengineering, Vol.84, No.5, 471-474, 1997. (Summary) 自然界から多様な大腸菌O157を分離し，それらの遺伝的多様性を明らかにすると共に，典型的細菌酵素であるアラニンラセマーゼの遺伝子断片との反応性を明らかにし，アラニンラセマーゼを指標とした大腸菌検査法が多様な大腸菌O157を検出できることを示した． (Link to Publication Site) ● Publication site (DOI): 10.1016/S0922-338X(97)82010-7 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0031444918 (DOI: 10.1016/S0922-338X(97)82010-7, Elsevier: Scopus)
53.	Yuka Isobe, Yoko Toyama, Mayumi Minamori, Kumio Yokoigawa, Kinji Endo, Fusako Kawai and Hiroyasu Kawai : Comparison of Physicochemical Properties of a Novel Polysaccharide Produced by Bacillus circulans with Commercial Polysaccharides., Nippon Shokuhin Kagaku Kogakukaishi, Vol.43, 634-641, 1996. (Summary) Bacillus circulans の生産する高粘性多糖の物理化学的性質を各種既知多糖と比較検討した．本菌の多糖は，親水性にすぐれ，また，高い乳化安定性を示した．粘性は，pHの変化に対しても安定であった．
54.	Kumio Yokoigawa, Rikako Tomioka, Yoko Okubo and Hiroyasu KAWAI : An Alanine Racemase Gene as a New Index for Detecting Escherichia coli in Foods., Bioscience, Biotechnology, and Biochemistry, Vol.60, No.11, 1899-1904, 1996. (Summary) 食品中の大腸菌を迅速かつ正確に検出するための指標として，細胞壁生合成に必須のアラニンラセマーゼ遺伝子が有用であることを明らかにした．本酵素の遺伝子断片をプローブとしたDNAハイブリダイゼーション法において，本プローブは大腸菌以外の細菌群とはほとんど反応性を示さず，病原性大腸菌を含む多様な大腸菌にきわめて特異的であった．大腸菌食品中でも，その特異性は保持されることが確認された．
55.	Yoko Murakami, Kumio Yokoigawa, Fusako Kawai and Hiroyasu KAWAI : Lipid Composition of Commercial Bakers' Yeasts Having Different Freeze-tolerance in Frozen Dough., Bioscience, Biotechnology, and Biochemistry, Vol.60, 1874-1876, 1996. (Summary) 酵母の冷凍耐性と脂質組成の関係を明らかにするため，冷凍耐性率の異なる各種市販パン酵母の脂質組成を分析した．総脂質量や各種リン脂質含量，脂肪酸組成は，各酵母で違いが見られなかったが，リン脂質に対するステロールの比率は，耐性株と感受性株で違いが見られ，冷凍耐性と膜流動性は相関するものと考えられた．
56.	Yoko Murakami, Kumio Yokoigawa, Kinji Endo and Hiroyasu Kawai : Lipid Composition of a Freeze-tolerant Yeast, Torulaspora delbrueckii, and Its Freeze-sensitive Mutant., Appl. Microbiol. Biotechnol., Vol.44, 167-171, 1995. (Summary) 冷凍耐性酵母と冷凍感受性酵母の脂質組成を比較分析し，両株の違いを明らかにした．特に，リン脂質に対するステロールの比率の違いから，冷凍耐性酵母は冷凍感受性酵母に比べ，膜の流動性が高いことが明らかとなった．
57.	Kumio Yokoigawa, Yoko Murakami and Hiroyasu Kawai : Trehalase Activity and Trehalose content in a Freeze-tolerant Yeast, Torulaspora delbrueckii and Its Freeze-sensitive Mutant., Bioscience, Biotechnology, and Biochemistry, Vol.59, 2143-2145, 1995. (Summary) 酵母の冷凍耐性に関与するトレハロースとその加水分解酵素トレハラーゼについて，冷凍耐性株とその冷凍感受性変異株を用いて比較分析した．冷凍感受性酵母は，冷凍耐性酵母に比べ，トレハラーゼ活性が高く，細胞内トレハロース含量も少ないことが明らかとなった．また，トレハラーゼには至適反応pHの異なる3種類の酵素が存在することが判明した．
58.	Yoko Okubo, Rikako Tomioka, Kumio Yokoigawa and Hiroyasu KAWAI : Lability of Alanine Racemase from a Psychrotroph., Journal of Home Economics of Japan, Vol.46, No.12, 1135-1140, 1995. (Summary) 増殖に必須な酵素の不活性化を通して低温菌の増殖を抑制することを目的として，典型的低温細菌である Pseudomonas fluorescens 由来アラニンラセマーゼの不安定性を，好熱菌，常温菌由来の本酵素と比較分析し，低温菌酵素はアルコール等の有機溶媒や界面活性剤により容易に不活化されることを明らかにした．
59.	Yoko Murakami, Youn Sook - Hahn, Kumio Yokoigawa, Kinji Endo and Hiroyasu Kawai : Induction of Freeze-sensitive Mutants from a Freeze-tolerant Yeast, Torulaspora delbrueckii., Bioscience, Biotechnology, and Biochemistry, Vol.58, 206-207, 1994. (Summary) 冷凍生地製パン法に不可欠な酵母の性質である冷凍耐性について，その機構を明らかにするため，冷凍耐性酵母から冷凍感受性酵母を変異誘導して，その冷凍感受性株を分離し諸性質を明らかにした．
60.	Kumio Yokoigawa, Etsuko Kimura, Nobuyoshi Esaki and Kenji Soda : Enantioselective Synthesis of N-Acetyl-L-methionine with Aminoacylase in Organic Solvent., Appl. Microbiol. Biotechnol., Vol.42, 287-289, 1994. (Summary) NーアシルL-アミノ酸やD-アミノ酸は，抗生物質の重要な成分として用いられている．本論文は，NーアシルL-アミノ酸とD-アミノ酸の両者を，ラセミ化アミノ酸から合成する効率的な方法を確立したものである．
61.	Kumio Yokoigawa, Hiroyasu Kawai, Kinji Endo, Yong Hee Lim, Nobuyoshi Esaki and Kenji Soda : Thermolabile Alanine Racemase from a Psychrotroph, Pseudomonas fluorescens: Purification and Properties., Bioscience, Biotechnology, and Biochemistry, Vol.57, 93-97, 1993. (Summary) 低温保存食品の品質低下を招く低温菌の熱に不安定な酵素を均一に精製し，その酵素化学的諸性質を明らかにした．本酵素は低温できわめて高い活性を有し，30℃以上の温度で容易に変性する典型的な低温性酵素であった．
62.	Yong Hee Lim, Kumio Yokoigawa, Nobuyoshi Esaki and Kenji Soda : A New Amino Acid Racemase with Threonine α-Epimerase Activity from Pseudomonas putida: Purification and Characterization., Journal of Bacteriology, Vol.175, 4213-4217, 1993. (Summary) うま味を有するトレオニンに対してα-エピメラーゼ活性を有する新規酵素を発見し，その酵素を均一に精製して，酵素化学的諸性質を明らかにした．本酵素は，他種類のアミノ酸をもラセミ化するユニークな酵素であった．
63.	Jeong Weon Huh, Kumio Yokoigawa, Nobuyoshi Esaki and Kenji Soda : Synthesis of L-Proline from the Racemate by Coupling of Enzymatic Enantiospecific Oxidation and Chemical Non-enantiospecific reduction., Journal of Fermentation and Bioengineering, Vol.74, No.3, 189-190, 1992. (Summary) 酵素的酸化反応と化学的還元反応の共役反応により，アミノ酸(プロリン)のラセミ体を特異的にL体に変換する方法を確立した．本法を用いれば，安価なラセミ体から，高収率でL-体を生産することが可能である． (Link to Publication Site) ● Publication site (DOI): 10.1016/0922-338X(92)90082-6 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0026673457 (DOI: 10.1016/0922-338X(92)90082-6, Elsevier: Scopus)
64.	Jeong Weon Huh, Kumio Yokoigawa, Nobuyoshi Esaki and Kenji Soda : Total Conversion of Racemic Pipecolic Acid into the L-Enantiomer by a Combination of Enantiospecific Oxidation with D-Amino Acid Oxidase and Reduction with Sodium Borohydride., Bioscience, Biotechnology, and Biochemistry, Vol.56, 2081-2082, 1992. (Summary) 抗菌物質の側鎖修飾剤として有用なL-ピペコリン酸の合成に，ラセミ化ピペコリン酸を材料として，D-アミノ酸酸化酵素と化学的還元剤の共役反応を用いることにより，特異的かつ高収率でL-ピペコリン酸が生成することを見いだした．
65.	Kumio Yokoigawa, Katsuyuki Tanizawa and Kenji Soda : Chemical Modification of Urokinase with Bis-imidoesters and Properties of the Intramolecularly Cross-linked Enzyme., Agricultural and Biological Chemistry, Vol.53, 1837-1842, 1989. (Summary) 二価性架橋試薬を用いる血栓溶解酵素(ウロキナーゼ)の分子内架橋により，耐熱性や血中安定性等の物理的·生物化学的性質を改善した．
66.	Kumio Yokoigawa, Katsuyuki Tanizawa and Kenji Soda : Chemical Modification of Urokinase with Human Serum Albumin Fragments., Agricultural and Biological Chemistry, Vol.53, 2887-2893, 1989. (Summary) 血液中での半減期が1分間以内という極めて不安定なウロキナーゼに，ヒト血清アルブミンの断片を化学的に結合させることにより，ウロキナーゼの免疫化学的性質をアルブミンに変換させると共に，血液中での安定性が極めて高い修飾酵素を作製した．
67.	W. Rogatti, Kumio Yokoigawa and T. Kobayashi : Urokinase Quality: Analysis of Different Preparations., Thrombosis and Haemostasis, Vol.53, 245-248, 1985. (Summary) 血栓溶解酵素の性質を酵素化学，生物化学，免疫化学的に解明した．特に，不純タンパク質に対する抗体を用いた純度分析は，微量の不純タンパク質の検出に有用であった．
68.	Kumio Yokoigawa, Katsuyuki Tanizawa and Kenji Soda : Purification of High Molecular Weight Urokinase by Reverse-immunoadsorption., Agricultural and Biological Chemistry, Vol.48, 1587-1593, 1984. (Summary) 人尿中に極めて微量存在する血栓溶解酵素ウロキナーゼを，本酵素以外の多種類のタンパク質に対する抗体を固定化した逆免疫吸着体を用いて，迅速かつ高収率で精製する新しい精製方法を確立した．

Academic Paper (Unrefereed Paper):

1.	Makiko Tsuji, Niro Takemasa and Kumio Yokoigawa : Effect of Microwave Radiation on Escherichia coli O157:H7 in foods, Natural Science Research, Faculty of Integrated Arts and Sciences, The University of Tokushima, Vol.27, No.3, 13-17, 2013. (Summary) We examined the survival and acid resistance of enterohemorrhagic Escherichia coli O157 (EHEC O157) in foods irradiated by microwave (2.45 GHz, 700 W, 7 s) with a domestic microwave oven. When the microwave irradiated foods inoculated with the cells at the cell density of 3 x 106 cells/g, the living cell number decreased below 65%. The cells in curry showed the lowest survival ratio (2%) among those in foods examined. Acid resistance of the cells in foods irradiated by the microwave was also effectively reduced below 10% irrespective of foods surveyed. Microwave could prove useful for reducing the living cell number and acid resistance of EHEC O157 in foods. (Tokushima University Institutional Repository) ● Metadata: 105957 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050564287418279424 (Tokushima University Institutional Repository: 105957, CiNii: 1050564287418279424)
2.	Makiko Tsuji, Tadashi Teramoto, Takatoshi Moriyama and Kumio Yokoigawa : Determination of Living Cells of Lactic Bacteria in Foods with Petri-film, Natural Science Research, Faculty of Integrated Arts and Sciences, The University of Tokushima, Vol.26, No.1, 1-6, 2012. (Summary) We examined the living cell number of lactic bacteria in foods by anaerobic culture with Petri-filmTM (PFAC).As the reference medium, the de Man, Rogosa and Sharpe agar medium (MRSA) and an agar mediumsupplemented with bromocresol purple (BCPA) were used. When we determined the living cell number oflactic bacteria in various products of pickled vegetables (29 products), kimuchi (10 products), meats (13products), lactic fermenting beverages (24 products) and cultured milks (6 products), the cell numbersdetermined with PFAC showed high correlations with those determined with MRSA and BCPA. (Tokushima University Institutional Repository) ● Metadata: 84179 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050845762393971584 (Tokushima University Institutional Repository: 84179, CiNii: 1050845762393971584)
3.	Mizue Miki, Kumi Tanimoto, Tomoko Shikata and Kumio Yokoigawa : Production of Human Type Glycosylated Tissue Plasminogen Activator and the Role of Its Carbohydrate Moiety, Natural Science Research, Faculty of Integrated Arts and Sciences, The University of Tokushima, Vol.22, 97-104, 2008. (Summary) To begin the examination of the role of human type carbohydrate moiety of tissue plasminogen activator(t-PA) on the binding of the enzyme to fibrin，the naturally glycosylated enzyme was produced by microcarrierculture of human cells established from normal uterine muscle. The cells grown on microcarrers in Haunks' MEM supplemented with 10 % FBS (1.6 x 10 6 cells/ml) rapidly detached themselves from microcarrier in aserum-free medium (t-PA production medium) within 5 days, and it was difficult to produce t-PA for long time (t-PA production: an average of 3 IU/ml/day over 5 days). Addition of 0.5% beef extract to the serum-freemedium suppressed their detaching from microcarriers. By regulating the pH (7.4) and dissolved oxygen(4 ppm) of the serum free medium，the cell density of microcarrier culture increased to 1.2 x10 7 cells/ml and t-PAwas produced over 38 days (t-PA production: an average of 836 IU/ml/day over 38 days). Native t-PAs purifiedto homogeneity from the culture broth had the molecular weight of 63,000 and 65,000 containing 5.6 and 8.5 %carbohydrate，respectively (molecular mass of protein moiety was calculated to be 59,500). By enzymaticdigestion of carbohydrate moiety in native t-PA，we obtained partially deglycosylated t-PA with molecularweights of 60,000 and 62,000. Completely deglycosylated t-PA was obtained by t-PA production in thepresence of 10μg/ml tunicamycin (N-glycosylation inhibitor). This suggests that N-glycosylationof t-PAoccurs while O-glycosylation is absent. The binding strength of these enzymes to fibrin increased withdecrease of the carbohydrate content. The carbohydrate moiety of human type glycosylated t-PA probablymodulates the binding strength of the enzyme to fibrin. (Keyword) Tissue plasminogen activator / cell culture / carbohydrate / animal cell / fibrinolysis (Tokushima University Institutional Repository) ● Metadata: 61875 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050564287417111168 (Tokushima University Institutional Repository: 61875, CiNii: 1050564287417111168)

Review, Commentary:

1.	Kaori Kanemaru and Kumio Yokoigawa : 大腸菌の食品加工・調理器具素材への付着とバイオフィルム形成, Clean Technology, Vol.28, No.8, 19-21, Aug. 2018. (Keyword) Escherichia coli / biofilm (Tokushima University Institutional Repository) ● Metadata: 113898 (Tokushima University Institutional Repository: 113898)
2.	Kaori Kanemaru, Makiko Tsuji and Kumio Yokoigawa : 食品加工・調理器具への微生物の付着と殺菌剤耐性, 食品機械装置, Vol.53, No.10, 50-55, Oct. 2016. (Tokushima University Institutional Repository) ● Metadata: 113902 (Tokushima University Institutional Repository: 113902)
3.	寺本忠司, 武政二郎 and Kumio Yokoigawa : 食品企業における自主細菌検査の基本技術, HACCP, Vol.15, No.5, 20-31, May 2009. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1522262180648087168 (CiNii: 1522262180648087168)
4.	Kumio Yokoigawa and 健次左右田 : Recent development in D-amino acid and amino asid racemase, バイオサイエンスとインダストリー, Vol.62, No.2, 17-22, Feb. 2004. (Summary) D-アミノ酸の分布と生理学的性質並びにアミノ酸ラセマーゼの構造と機能に関する研究の新局面をまとめた総説である． (Keyword) D-アミノ酸 / 異性化酵素 / D-アミノ酸ポリマー / アラニンラセマーゼ
5.	Kumio Yokoigawa : 冬の食中毒, クリンネス(イカリ環境事業グループ・環境文化創造研究所機関誌), Vol.12, 32-36, Dec. 2002. (Summary) 近年の食中毒は冬場でも多発することを，病原体毎に解説した．
6.	Kumio Yokoigawa and 健次左右田 : Psychrotrophic Enzymes, BIO INDUSTRY, Vol.16, No.10, 40-47, Oct. 1999. (Summary) 著者等の低温性アラニンラセマーゼに関する研究を中心に，低温性酵素の基礎と応用をまとめた総説．
7.	Yoko Okubo and Kumio Yokoigawa : Evaluation of alanine racemase gene as a new indicator for detecting bacteria in food, Recent Res. Devel. Ferment. Bioeng., Vol.2, 2-19, Feb. 1999. (Summary) 食品中に存在する種々の細菌を，迅速かつ正確に検出するための指標として，細菌固有の酵素であるアラニンラセマーゼを検討し，様々な細菌を検出するための指標として有用であることを示した総説．
8.	Kumio Yokoigawa : 大腸菌O157について, 日本調理科学会誌, Vol.31, No.2, 152-156, Feb. 1998. (Summary) 大腸菌O157の特性，検査法，感染源，予防対策などを解説した．
9.	Kumio Yokoigawa and Hiroyasu Kawai : Difference between freeze-tolerant and freeze-sensitive bakers' yeasts in their DNA, 冷凍, Vol.72, No.841, 61-65, Nov. 1997. (Summary) DNA解析の一般法を解説すると共に，著者等が行ったパルスフィールドゲル電気泳動法による市販の冷凍耐性パン酵母と冷凍感受性パン酵母のDNAの違いを解説した．
10.	Kumio Yokoigawa : 食品の細菌学的検査におけるバイオテクノロジー, New Food Industry, Vol.37, No.11, 32-38, Nov. 1995. (Summary) 食品の細菌学的検査法の中で，最も重要かつ汎用されている大腸菌検査と一般細菌数について，酵素法，培養法等の現状と問題点，さらに著者等の原核性酵素アラニンラセマーゼを指標とする最新の方法などをまとめた総説．
11.	Kumio Yokoigawa and 健次左右田 : トパキノン, Seikagaku, Vol.63, 543, Jul. 1991. (Summary) 牛血清銅含有アミンオキシダーゼに見いだされた新しい補酵素トパキノンの解説．

Proceeding of International Conference:

1.	Kumio Yokoigawa, Tsuji Makiko and Goto Tsukie : Adsorption of shiga toxin to foods containing indigestible components., Pacifichem2015, Honolulu, Dec. 2015.
2.	Kumio Yokoigawa, Makiko Tsuji and Nirou Takemasa : Attachment of Escherichia coli O157 to abiotic surfaces, PacifiChem2010, Honolulu, Dec. 2010.
3.	Natsuko Kusunoki, Kozue Edo, Kaori Ohnishi, Keiko Ashida, Asami Nakanishi, Hitomi Sakai, Hideyuki Nakagawa, Kumio Yokoigawa, Haruhiko Sakuraba and Toshihisa Ohshima : Multiple lectins from globiferous pedicellariae of the sea urchin, Toxopneustes pileolus, The Proceeding of 21st Pacific Science Congress:Session:4-10 Health hazard by marine animals in Pacific region, 7p, Okinawa, Jun. 2007. (Keyword) lectin / sea urchin / venom / Toxopneustes pileolus / pedicellariae
4.	Kumio Yokoigawa : Properties of psychrophilic alanine racemase, 2005 International Chemical Congress, Honolulu, Dec. 2005.
5.	Kumio Yokoigawa : Characterization of monomeric alanine racemase in dynamic equilibrium, International interdisciplinary conference on vitamins,coenzymes,and biofactors, Awaji Kobe,Japan, Nov. 2005.
6.	Naomi Mizobuchi, Kumio Yokoigawa, Terue Harumoto, Hiromi Fujisawa and Yoshiomi Takagi : Hydrogen peroxide and catalase are respectively the paramecia-killing toxin in wheat-grass-powder infusion and the bacteri-derived detoxifying substance, 4th European Congress of Protistology and 10th European Congress on Ciliate Biology, 93, San Benedetto del Tronte,Italy, Sep. 2003.
7.	Yoko Okubo, Kumio Yokoigawa, Kenji Soda and Haruo Misono : Effect of PLP on stability of psychrophilic alanine racemase, 2000 International Chemical Cogress, Honolulu, Dec. 2000.
8.	Kumio Yokoigawa and Yoko Okubo : Difference between alanine racemases from Shigella species and E.coli., 2000 International Chemical Congress, Honolulu, Dec. 2000.
9.	Kumio Yokoigawa, Yoko Okubo, Nobuyoshi Esaki, Kenji Soda and Hiroyasu Kawai : Structure and function of Psychrophilic Alanine Racemase from Bacillus psychrosaccharolyticus, 10th International symposium on Vitamin B6 and carbonyl catalysis, Santa Fe New Mexico,USA, Nov. 1999.
10.	Kumio Yokoigawa : Bacteriological analysis of foods using alanine racemase gene as an indesx, Korea-Japan Workshop on Biocatalysis, Kyonju,Korea, Jun. 1996.
11.	Kumio Yokoigawa, Yoko Okubo and Hiroyasu Kawai : Amplification of gene fragment of alanine racemase and its application to detection of bacteria in foods, 1995 International Chemical Congress, Honolulu, Dec. 1995.
12.	Kenji Soda, Kumio Yokoigawa, Jeong Weon Huh and Nobuyoshi Esaki : A 340 nm chromophore of nitroalkane oxidase, 2nd International Symposium on PQQ and Quinoproteins, Yamaguchi, Nov. 1991.
13.	Kumio Yokoigawa, Nobuyoshi Esaki and Kenji Soda : Properties of alanine racemase from a phychorotrophic bacterium, The first International Congress on Vitamines and Biofactors in Life Science, Kobe, Sep. 1991.
14.	Hiroyuki Ashida, Tadao Oikawa, Kumio Yokoigawa, Nobuyoshi Esaki and Kenji Soda : A selenocysteine analogue of glutathione,glutaselenone : glutathione peroxidase activity and reaction mechanism, The 6th International Conference on the Chemistry of Selenium and Tellurium, Osaka, Jun. 1991.
15.	Kumio Yokoigawa, Jeong Weon Huh, Takeo Moriyta, Katsuyuki Tanizawa, Nobuyoshi Esaki and Kenji Soda : A cofactor of nitroalkane oxidase, International Symposium on VitaminB6 and Carbonyl Catalysis, Osaka, Oct. 1990.

Proceeding of Domestic Conference:

1.	Hitomi Sakai, Tohru Sakai and Kumio Yokoigawa : 身の回りの素材に対する小麦粉由来アラビノキシランの大腸菌付着抑制効果について, 第43回日本食品微生物学会, Sep. 2022.
2.	Hitomi Sakai, Kaori Kanemaru, Tohru Sakai and Kumio Yokoigawa : 調理器具素材への大腸菌の付着を抑制する小麦粉の成分の同定, 第39回日本食品微生物学会総会, Sep. 2018.
3.	Kaori Kanemaru, Hitomi Sakai, Keiko Takahashi and Kumio Yokoigawa : ガラス表面に付着した大腸菌の洗浄と殺菌, 第39回日本食品微生物学会学術総会講演要旨集, 111, Sep. 2018. (Keyword) biofilm / Escherichia coli
4.	矢野圭介, 佐藤拓郎, 後藤仁, 脇田美幸, 岡久修己, 西岡浩樹, 那須正夫 and Kumio Yokoigawa : バイオフィルム検査用スワブの評価, 第39回日本食品微生物学会学術総会講演要旨集, 29, Sep. 2018. (Keyword) biofilm
5.	Ryushi Kawakami, Kaori Kanemaru and Kumio Yokoigawa : 食品微生物や発酵食品に含まれるD-アミノ酸検出のためのUHPLC検出系の構築, 第38回日本日本食品微生物学会学術総会, Oct. 2017.
6.	Badr Ali Hoida, Takahashi Keiko, Ryushi Kawakami, Yasuo Oyama, Kumio Yokoigawa and Kaori Kanemaru : Screening and analysis of edible seaweeds in the ability to adsorb Shiga toxin, 第38回日本日本食品微生物学会学術総会, Oct. 2017.
7.	Tsukie Goto, Kaori Kanemaru and Kumio Yokoigawa : 固定化ポリーγーグルタミン酸の志賀毒素吸着性, 第38回日本食品微生物学会学術総会, Oct. 2017.
8.	Hitomi Sakai, Tohru Sakai and Kumio Yokoigawa : 調理器具素材への大腸菌の付着を抑制する小麦粉の成分について, 第38回日本食品微生物学会学術総会, Oct. 2017.
9.	生杉笙, Kaori Kanemaru, Kumio Yokoigawa and Yasuo Oyama : 徳島県産食用植物クサギClerodendrum trichotomunのガン細胞増殖抑制, 日本農芸化学会中国四国支部第48回講演会講演要旨, 33, Jun. 2017.
10.	Kumio Yokoigawa, 高橋啓子 and Kaori Kanemaru : 非生物素材に付着した大腸菌O157のバイオフィルム形成, 日本農芸化学会2017大会, Mar. 2017.
11.	Kaori Kanemaru, 生杉笙, Ryushi Kawakami, Yasuo Oyama and Kumio Yokoigawa : 徳島県産食用植物のガン細胞増殖抑制, 日本農芸化学会2017年度(平成29年度)大会講演要旨集, Mar. 2017.
12.	Kumio Yokoigawa, オワイダアリバドル and Kaori Kanemaru : 食用海藻の志賀毒素吸着性, 第37回日本食品微生物学会学術総会講演要旨集, 64, Sep. 2016.
13.	Hitomi Sakai, Tohru Sakai and Kumio Yokoigawa : 調理器具素材への大腸菌の付着に及ぼす穀類の影響, 第37回日本食品微生物学会学術総会講演要旨集, 111, Sep. 2016.
14.	Hitomi Sakai, Tohru Sakai and Kumio Yokoigawa : 大腸菌の非生物素材への付着に対する穀類の影響, 日本農芸化学会中四国支部第45回講演会要旨集, 23, Jun. 2016.
15.	後藤月江, 逵牧子, Kaori Kanemaru and Kumio Yokoigawa : 沈殿したポリ-γ-グルタミン酸の志賀毒素吸着性, 日本農芸化学会中四国支部第45回講演会要旨集, 24, Jun. 2016.
16.	高橋啓子, Takefumi Hattori, Kaori Kanemaru, Yasuo Oyama and Kumio Yokoigawa : Tricholoma matsutake 菌糸生育におけるD-及びL-アミノ酸の添加効果, 日本農芸化学会中四国支部第45回講演会要旨集, 36, Jun. 2016.
17.	Badr Ali Hoida, Kaori Kanemaru and Kumio Yokoigawa : Screening and analysis of edible seaweeds in the ability to adsorb Shiga toxin, 日本農芸化学会中四国支部第45回講演会要旨集, 23, Jun. 2016.
18.	Hiromitsu Tsuzuki, Kumio Yokoigawa, 阿部正範, 太田明 and Takefumi Hattori : (E)-, 第65回日本木材学会大会研究発表要旨集，東京都タワーホール船堀，東京都，2015年3月16日から18日, Mar. 2015.
19.	Takefumi Hattori, 都築弘充, 天羽宏枝, Kumio Yokoigawa, 阿部正範 and 太田明 : マツタケにおける(E)-ケイ皮酸メチル生合成経路, 日本きのこ学会第18回大会, 136, Sep. 2014.
20.	Kumio Yokoigawa, 逵牧子 and 武政二郎 : 食品及び食物繊維のベロ毒素吸着性, 第34回日本食品微生物学会学術総会講演要旨集, 41, Oct. 2013.
21.	Kumio Yokoigawa and 逵牧子 : 非生物素材に付着した大腸菌O157の生存性, 日本農芸化学会2013年度合同広島大会講演要旨集, 95, Sep. 2013.
22.	Kumio Yokoigawa : 食品製造設備の汚れに関連する微生物について, 神戸食品微生物研究会産学連携研究発表会特別講演, Mar. 2013.
23.	Asuka Nakajima and Kumio Yokoigawa : Effect of citrus fruits on enterobacteria, 日本農芸化学会中四国支部第35回講演会講演要旨集, 20, Jan. 2013.
24.	Kumio Yokoigawa : Agricultural chemistry in University of Tokushima and Future of Research on Escherichia coli O157, 日本農芸化学会中四国支部創立10周年記念シンポジウム講演要旨集, 14, Sep. 2012.
25.	浮田健太郎, 新井公誉 and Kumio Yokoigawa : 酵母の発酵力に対するD-システインの影響, 日本農芸化学会中四国支部第29回講演会要旨集, 34, Jan. 2011.
26.	Kumio Yokoigawa, 麻野亜耶佳, 石本麻衣子, 武政二郎 and 逵牧子 : 大腸菌O157の調理器具に対する付着性の抑制, 日本農芸化学会中四国支部大会講演要旨集, 48, Sep. 2010.
27.	逵牧子, 寺本忠司 and Kumio Yokoigawa : ペトリフイルムACプレートを用いる乳酸菌数測定の評価, 日本家政学会第62回大会講演要旨集, Vol.62, 13, May 2010.
28.	武政二郎, 逵牧子, 田中文乃 and Kumio Yokoigawa : 大腸菌O157の個体表面への付着性, 日本農芸化学会2010年度大会, 68, Mar. 2010.
29.	田中文乃 and Kumio Yokoigawa : 大腸菌O157のベロ毒素生産に対するオートインデューサーの影響, 日本農芸化学会中四国支部第26回講演会, 13, Jan. 2010.
30.	逵牧子, 武政二郎 and Kumio Yokoigawa : マイクロ波照射培養による大腸菌O157のベロ毒素生産性の変動, 日本農芸化学会関西・中四国・西日本支部2009年度合同沖縄大会, 138, Oct. 2009.
31.	逵牧子, 武政二郎 and Kumio Yokoigawa : 食品に混入した大腸菌O157に対するマイクロ波の影響, 第82回日本生化学会大会, Oct. 2009.
32.	梅迫誠一, 百武晃宏, 加藤孝広, 武政二郎 and Kumio Yokoigawa : 培地調製を簡略化した「プチットカンピロ(プレストン)」の評価, 第30回日本食品微生物学会学術総会, 53, Oct. 2009.
33.	逵牧子, 武政二郎 and Kumio Yokoigawa : マイクロ波照射による温度制御の下で生育した大腸菌O157の特徴, 第30回日本食品微生物学会学術総会, 97, Oct. 2009.
34.	Kumio Yokoigawa : 電子レンジのマイクロ波が大腸菌O157に及ぼす影響, 日本家政学会第61回大会, 124, Aug. 2009.
35.	西谷雅人, 松本和也 and Kumio Yokoigawa : 大腸菌O157の生細胞数，酸耐性及びべロ毒素生産性に対する電子レンジの影響, 日本農芸化学会2009年度大会, 241, Mar. 2009.
36.	松本和也, 西谷雅人 and Kumio Yokoigawa : 大腸菌O157のべロ毒素生産性と酸耐性に対するマイクロ波の影響, 日本農芸化学会中四国支部第23回講演会, 27, Jan. 2009.
37.	谷本久美, 古川雅恵, 大西浩平, Hideaki Tsuge and Kumio Yokoigawa : 低温性および常温性Pseudomonas属細菌由来アラニンラセマーゼの比較研究, 日本農芸化学会中四国支部第23回講演会, 21, Jan. 2009.
38.	Kumio Yokoigawa, 池田麻乃 and 大西伸弥 : 大腸菌O157に対するマイクロ波の影響, 第31回日本分子生物学会第81回日本生化学会大会合同大会, 820, Dec. 2008.
39.	Kumio Yokoigawa : 大腸菌O157に対する香辛料とマイクロ波の影響, 第29回日本食品微生物学会学術総会, 65, Nov. 2008.
40.	Kumio Yokoigawa, 大西浩平 and Hideaki Tsuge : 低温細菌Pseudomonas fluorescens及び常温細菌Pseudomonas aeruginosa由来アラニンラセマーゼの比較, 第4回D-アミノ酸研究会学術講演会, 33, Sep. 2008.
41.	Kumio Yokoigawa, 池田麻乃 and 大西伸弥 : 香辛料製油成分とマイクロ波による大腸菌O157の弱毒化, 第49回日本生化学会中国・四国支部例会, May 2008.
42.	Kumio Yokoigawa : 大腸菌O157のベロ毒素生産に対する香辛料製油成分の影響, 日本農芸化学会2008年度大会, 157, Mar. 2008.
43.	三木瑞枝 and Kumio Yokoigawa : 酵母の増殖と発酵力に対するスダチの影響, 日本農芸化学会中四国支部第20回講演会, Jan. 2008.
44.	大西伸弥, 池田麻乃, 小野田協子 and Kumio Yokoigawa : 香辛料製油成分とマイクロ波の組合せによる大腸菌O157の弱毒化, 日本農芸化学会中四国支部第20回講演会, Jan. 2008.
45.	Kumio Yokoigawa and 佐藤真智子 : ポリガンマーグルタミン酸の抗変異原活性, 第30回日本分子生物学会年会第80回日本生化学会大会合同大会, 865, Dec. 2007.
46.	Kumio Yokoigawa : 香辛料による大腸菌O157の弱毒化, 日本農芸化学会中四国・西日本支部合同大会, Sep. 2007.
47.	Kumio Yokoigawa : 食をとりまく微生物危害の現状, 第28回日本食品微生物学会学術セミナー, 1-8, Jul. 2007.
48.	Kumio Yokoigawa : 食品関連物質による微生物の制御, 日本農芸化学会中四国支部創設5周年記念事業「徳島における生物資源研究」特別講演会, Mar. 2007.
49.	Kumio Yokoigawa and 鈴江あゆみ : 数種類の酵素に対するマイクロ波の影響, 日本ビタミン学会第58回大会, Vol.80, No.4, 240, May 2006.
50.	Kumio Yokoigawa and 新井公誉 : 単量体アラニンラセマーゼの二量体形成阻害による活性阻害, 日本生化学会大会, Oct. 2005.
51.	Kumio Yokoigawa : 大腸菌O157のベロ毒素生産に対する香辛料成分の影響, 日本家政学会2005年度大会, May 2005.
52.	Kumio Yokoigawa and 左右田健次 : パン酵母に対する電磁波の影響, 日本農芸化学会大会, Mar. 2005.
53.	Kumio Yokoigawa : 食品の製造現場で日常的に出現する微生物の基礎知識, コープ四国「食の安心安全研修会」, Feb. 2005.
54.	Kumio Yokoigawa : 大腸菌O157のベロ毒素生産に対する香辛料の影響, 日本農芸化学会中四国支部大会, Sep. 2004.
55.	Kumio Yokoigawa : 大腸菌O157の弱毒化と殺菌, 徳島生物学会シンポジウム, Jul. 2004.
56.	OJiansong Ju1, Kouhei Ohnishi, Kumio Yokoigawa and Haruo Misono : Characterization of dimer formation of bacterial alanine racemases, 日本農芸化学会2004年度大会, Mar. 2004.
57.	佐藤真智子, Kumio Yokoigawa and 左右田健次 : パン酵母の冷凍耐性と発酵力に対するポリガンマーグルタミン酸の影響, 日本農芸化学会2003年度大会, Apr. 2003.
58.	Kumio Yokoigawa : 食品細菌検査の迅速化に向けて─遺伝子診断技術の応用, 第18回日本食品微生物学会学術セミナー, Nov. 2002.
59.	Kumio Yokoigawa : 食品中で増殖した大腸菌O157の酸耐性とグルタミン酸脱炭酸酵素mRNAレベルの相関性, 日本農芸化学会関西支部大会, Oct. 2002.
60.	Kumio Yokoigawa : 低温細菌と常温細菌の香辛料に対する感受性の違い, 日本家政学会2002年度大会, 2002.
61.	Kumio Yokoigawa, 梅迫誠一 and 左右田健次 : 単量体アラニンラセマーゼのサブユニット相互作用, 日本農芸化学会2002年度大会, 2002.
62.	Kumio Yokoigawa, 滝川亜紀子 and 梅迫誠一 : 大腸菌O157に対するナツメグの殺菌効果, 日本生化学会近畿支部大会, May 2001.
63.	滝川亜紀子 and Kumio Yokoigawa : 大腸菌O157の生存に対するスパイスの影響, 日本家政学会2001年度大会, May 2001.
64.	Kumio Yokoigawa, 滝川亜紀子 and 梅迫誠一 : 大腸菌O157の酸耐性に対する増殖温度と食品の影響, 日本食品微生物学会2001年度大会, 2001.
65.	Kumio Yokoigawa and 大久保陽子 : 低温性アラニンラセマーゼ遺伝子の大腸菌での発現に対する培養温度の影響, 日本農芸化学会2001年度大会, 2001.
66.	滝川亜紀子, Kumio Yokoigawa and Hiroyasu Kawai : 大腸菌O157:H7の食品中での増殖特性と酸耐性の変動, 日本家政学会2000年度大会, Jun. 2000.
67.	Kumio Yokoigawa : 食品の流通温度における細菌の増殖態度とその制御, 第12回食品微生物学会学術セミナー, Mar. 2000.
68.	平澤玲子, 磯部由香, Kumio Yokoigawa and Hiroyasu Kawai : パン酵母細胞へのトレハロースの取り込みと生存率および生地発酵力との関連性, 日本農芸化学会2000年度大会, 2000.
69.	大久保陽子, Kumio Yokoigawa, 左右田健次 and 味園春雄 : 低温性アラニンラセマーゼの補酵素による安定化, 日本農芸化学会2000年度大会, 2000.
70.	Kumio Yokoigawa and Hiroyasu Kawai : 赤痢菌4亜属由来アラニンラセマーゼ遺伝子の配列, 日本農芸化学会2000年度大会, 2000.
71.	滝川亜紀子, Kumio Yokoigawa and 河合弘康 : 大腸菌O157と非病原性大腸菌の違い-調味料中での増殖と生存-, 日本家政学会1999年度関西支部研究発表会, Oct. 1999.
72.	梅迫誠一, 直井裕, 木本聖子 and Kumio Yokoigawa : 大腸菌O157以外の腸管出血性大腸菌検出法の検討, 日本食品微生物学会1999年度大会, Oct. 1999.
73.	Kumio Yokoigawa : 大腸菌O157の増殖と生存に対する食品成分の影響, 第52回神戸食品微生物研究会, Aug. 1999.
74.	滝川亜紀子, Kumio Yokoigawa and 河合弘康 : 大腸菌O157の生存能力と酸耐性に対する食品成分の影響, 日本家政学会1999年度大会, May 1999.
75.	大久保陽子, 左右田健次 and Kumio Yokoigawa : 低温菌由来アラニンラセマーゼのin vivoおよびin vitroでの不安定性, 日本農芸化学会1999年度大会, Apr. 1999.
76.	Kumio Yokoigawa, 大久保陽子 and 河合弘康 : アラニンラセマーゼを指標とする牛乳の無菌試験, 日本農芸化学会1999年度大会, Apr. 1999.
77.	平澤玲子, Kumio Yokoigawa and 河合弘康 : パン酵母の発酵力を高めるための前処理条件の検討, 日本家政学会関西支部会1999年, 1999.
78.	梅迫誠一, 直井裕, 木本聖子, 中川博司, 杉山淳, 上田義男 and Kumio Yokoigawa : 1次·2次抗原抗体反応による大腸菌血清型O157以外の腸管出血性大腸菌検査法の検討, 第19回奈良県公衆衛生学会, Nov. 1998.
79.	Kumio Yokoigawa : 大腸菌O157と非病原性大腸菌, 第8回日本応用糖質科学会近畿支部講演会, Oct. 1998.
80.	Kumio Yokoigawa, 大久保陽子 and 河合弘康 : 低温菌Bacillus psychrosaccharolyticus 由来アラニンラセマーゼの構造と機能, 日本家政学会1998年度大会, May 1998.
81.	Nazneen Bagum, Kumio Yokoigawa and Hiroyasu Kawai : Trehalose and its metabolism in bakers' yeast cells exposed to salt stress, 日本農芸化学会1998年度大会, Apr. 1998.
82.	大久保陽子, Kumio Yokoigawa and 河合弘康 : Bacillus circulans により生産される多糖の抗変異原性, 日本農芸化学会1998年度大会, Apr. 1998.
83.	大久保陽子, Kumio Yokoigawa, 江崎信芳, 左右田健次 and 河合弘康 : 低温菌Bacillus psychrosaccharolyticus由来アラニンラセマーゼの精製と性質, 日本農芸化学会1998年度大会, Apr. 1998.
84.	奈良香, Kumio Yokoigawa and 河合弘康 : アラニンラセマーゼ遺伝子を指標としたPCR法による大腸菌の特異的検出, 日本農芸化学会1998年度大会, Apr. 1998.
85.	Kumio Yokoigawa, 大久保陽子 and 河合弘康 : アラニンラセマーゼ遺伝子断片の細菌検出におけるプローブとしての特異性, 日本農芸化学会1997年度大会, Apr. 1997.
86.	大久保陽子, Kumio Yokoigawa, 河合弘康, 江崎信芳 and 左右田健次 : コンセンサスプライマーを用いた低温菌Bacillus psychrosaccharolyticus由来アラニンラセマーゼ遺伝子の構造解析, 日本農芸化学会1997年度大会, Apr. 1997.
87.	大久保陽子, Kumio Yokoigawa, 河合弘康, 江崎信芳 and 左右田健次 : 低温菌由来アラニンラセマーゼ遺伝子の解析, 日本農芸化学会1996年度大会, 1996.
88.	富岡里佳子, Kumio Yokoigawa and 河合弘康 : DNAハイブリダイゼーション法による簡便な食品の大腸菌検査, 日本農芸化学会大会, Aug. 1995.
89.	大久保陽子, Kumio Yokoigawa and 河合弘康 : 食品汚染細菌のPCR法による検出, 日本農芸化学会大会, Aug. 1995.
90.	富岡里佳子, Kumio Yokoigawa, 遠藤金次 and 河合弘康 : アラニンラセマーゼ遺伝子を指標とした食品の大腸菌検査に関する研究, 日本家政学会1995年度大会, May 1995.
91.	大久保陽子, Kumio Yokoigawa, 遠藤金次 and 河合弘康 : ポリメラーゼチェインリアクションによるアラニンラセマーゼ遺伝子の検出に関する研究, 日本家政学会1995年度大会, May 1995.
92.	大久保陽子, 富岡里佳子, Kumio Yokoigawa, 河合弘康, 遠藤金次, 江崎信芳 and 左右田健次 : 低温菌由来アラニンラセマーゼの特性, 日本農芸化学会大会, Apr. 1994.
93.	Kumio Yokoigawa, 大久保陽子, 富岡里佳子, 河合弘康 and 遠藤金次 : 低温菌由来アラニンラセマーゼの安定性について, 日本家政学会1993年度大会, May 1993.
94.	Kumio Yokoigawa, 河合弘康, 江崎信芳 and 左右田健次 : 低温菌由来アラニンラセマーゼの精製と性質, 日本農芸化学会大会, Apr. 1992.
95.	許正元, Kumio Yokoigawa, 江崎信芳 and 左右田健次 : ニトロアルカンオキシダーゼの含有する発色団について, 日本農芸化学会，中部支部·関西支部合同大会, Oct. 1991.
96.	Kumio Yokoigawa, 許正元, 守屋岳郎, 谷澤克行, 江崎信芳 and 左右田健次 : ニトロアルカンオキシダーゼ:PQQ類縁新規補酵素, 1991年日本農芸化学会シンポジウム，``新しいビタミン，PQQ:その機能とキノプロテイン'', 1991.
97.	深尾偉晴, 松尾睦美, 松尾理, Kumio Yokoigawa, 秋葉清 and 大村武雄 : 子宮筋肉由来の細胞株の分泌するプラスミノーゲンアクチベーターの特性, 日本組織培養学会大会, 1986.
98.	呟承烋, Jamshidi K., 筏義人, Kumio Yokoigawa, 左右田健次, 滝和郎, 小林映 and 半田肇 : 乳酸重合体からのウロキナーゼの除放, 日本薬学会大会, 1983.
99.	Kumio Yokoigawa and 左右田健次 : アルブミンフラグメントを用いるウロキナーゼの化学修飾, 日本農芸化学会大会, 1983.
100.	Kumio Yokoigawa, 谷澤克行 and 左右田健次 : 逆抗体アフィニティクロマトグラフィーによるウロキナーゼの精製, 日本農芸化学会大会, Apr. 1982.
101.	Kumio Yokoigawa, 谷澤克行 and 左右田健次 : 分子内架橋ウロキナーゼの性質, 日本生化学会大会, 1982.
102.	Kumio Yokoigawa, 谷澤克行 and 左右田健次 : ウロキナーゼの架橋反応による修飾, 昭和56年日本生化学会大会, 1981.

Products:

1.	Kumio Yokoigawa, 寺本忠司 and 武政二郎 : 製造現場にやさしい食品細菌検査, Feb. 2009.

Et cetera, Workshop:

1.	Kumio Yokoigawa : 微生物の食品加工・調理器具への付着と殺菌剤耐性, 日本食品工業倶楽部-食品の品質保証懇話会, Feb. 2016.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

難消化性食品成分の志賀毒素の吸着性 (Project/Area Number: 18K02204 )
Behavior of Escherichia coli O157 via cooking utensils (Project/Area Number: 15K00784 )
Effect of microwave on patho genicity of Escherichia coli O157 (Project/Area Number: 19300252 )
Study on attenuation of Escherichia coli 0157 with a combination of essential oil of spices. (Project/Area Number: 15300254 )
Specific sterilization of psychrotrophs in foods by inactivation of alanine racemase (Project/Area Number: 12480026 )
Growth and verocytotoxin productivity of Escherichia coli O157 grown at low temperature in foods (Project/Area Number: 10480021 )
Effect of Food Components on Growth of Escherichia coli O157 (Project/Area Number: 09358001 )
Studies on the Characterization of Novel Acidic Polysaccharide Produced by Bacteria for the Application to Food Industry (Project/Area Number: 08558002 )
Study on growth inhibition of psychrotrophs in foods on the basis of properties of a psychrotrophic enzyme (Project/Area Number: 08458005 )
Chinese-Japnese Co-operative University Research Program for Living Environment (Project/Area Number: 08045038 )
Correlation Between Freeze-tolerance of Bakers' Yeasts and Their Cellular Content of Trehalose (Project/Area Number: 07680019 )
Monitoring of Psychrotrophs in Foods by Polymerase Chain Reaction (Project/Area Number: 06680019 )
The Study on the Mechanisms of Freeze-tolerance of Yeast in Frozen-dough. (Project/Area Number: 05680021 )
ソルビン酸からの石油臭成分の生成に関する研究 (Project/Area Number: 05680020 )
Studies on Detection of Bacteria in foods with Oligonudeotides Derived from Alanine Racemase Gene (Project/Area Number: 05558002 )
Enzymochemical Study on growth of Psychrotrophs during Food Preservation in Refrigirator (Project/Area Number: 04680070 )
Design of New Selenium Enzymes and Proteins, and Their Functions (Project/Area Number: 02454545 )
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Professor Emeritus : Yokoigawa, Kumio

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)