Tokushima University / Educator and Researcher Directory --- Kitamura, Mitsuo

Field of Study

Subject of Study

Book / Paper

Academic Paper (Judged Full Paper):

1.	Mitsuo Kitamura : Reading FCS files and plotting the data using a free ware program, Journal of Medical Technology, Vol.62, No.6, 774-778, 2018. (Keyword) フローサイトメトリー / fcs (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282763013625600 (CiNii: 1390282763013625600)
2.	Kazuyoshi Kitaoka, Shiyori Kawata, Tomohiro Yoshida, Fumiya Kadoriku and Mitsuo Kitamura : Exposure to an Extremely-Low-Frequency Magnetic Field Stimulates Adrenal Steroidogenesis via Inhibition of Phosphodiesterase Activity in a Mouse Adrenal Cell Line, PLoS ONE, Vol.11, No.4, e0154167, 2016. (Summary) Extremely low-frequency magnetic fields (ELF-MFs) are generated by power lines and household electrical devices. In the last several decades, some evidence has shown an association between ELF-MF exposure and depression and/or anxiety in epidemiological and animal studies. The mechanism underlying ELF-MF-induced depression is considered to involve adrenal steroidogenesis, which is triggered by ELF-MF exposure. However, how ELF-MFs stimulate adrenal steroidogenesis is controversial. In the current study, we investigated the effect of ELF-MF exposure on the mouse adrenal cortex-derived Y-1 cell line and the human adrenal cortex-derived H295R cell line to clarify whether the ELF-MF stimulates adrenal steroidogenesis directly. ELF-MF exposure was found to significantly stimulate adrenal steroidogenesis (p < 0.01-0.05) and the expression of adrenal steroid synthetic enzymes (p < 0.05) in Y-1 cells, but the effect was weak in H295R cells. Y-1 cells exposed to an ELF-MF showed significant decreases in phosphodiesterase activity (p < 0.05) and intracellular Ca2+ concentration (p < 0.01) and significant increases in intracellular cyclic adenosine monophosphate (cAMP) concentration (p < 0.001-0.05) and cAMP response element-binding protein phosphorylation (p < 0.05). The increase in cAMP was not inhibited by treatment with NF449, an inhibitor of the Gs alpha subunit of G protein. Our results suggest that ELF-MF exposure stimulates adrenal steroidogenesis via an increase in intracellular cAMP caused by the inhibition of phosphodiesterase activity in Y-1 cells. The same mechanism may trigger the increase in adrenal steroid secretion in mice observed in our previous study. (Tokushima University Institutional Repository) ● Metadata: 112306 (Link to Publication Site) ● Publication site (DOI): 10.1371/journal.pone.0154167 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27100201 ● Summary page in Scopus @ Elsevier: 2-s2.0-84964653269 (Tokushima University Institutional Repository: 112306, DOI: 10.1371/journal.pone.0154167, PubMed: 27100201, Elsevier: Scopus)
3.	Toshitaka Ikehara, Mutsumi Nakahashi, Zehong Su, Masatake Akutagawa, Koichiro Tsuchiya, Mitsuo Kitamura, Akira Takahashi and Yohsuke Kinouchi : Effects of UV-A LED light irradiation on growth of cultured RAW 264.7 cells, Toxicological and Environmental Chemistry, Vol.97, No.2, 243-255, 2015. (Keyword) 365 nm / LED light / reactive oxygen species / Raw 264.7 cells / scavenger (Link to Publication Site) ● Publication site (DOI): 10.1080/02772248.2015.1039771 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84931571408 (DOI: 10.1080/02772248.2015.1039771, Elsevier: Scopus)
4.	Mitsuo Kitamura, 早野尚志 and Kazuo Yoshizaki : Re-design and evaluation of the NMR probes for living organs and tissues, Shikoku Acta Medica, Vol.69, No.1,2, 67-72, 2013. (Summary) We have constructed NMR signal detectors (probes) to measure the NMR spectra of living organs and tissues kept under physiological conditions. The probes consisted of non-magnetic aluminum alloy cylinder to support the detection coil made of cupper, mounted on a Teflon plate with acrylics plates and rods, however, the recovery time to the static magnetic field after turning off of the pulsed gradient field was more than 10 times longer than that of the micro-MRI probe available commercially. The re-design of our probes, such as acrylics cylinder to support the coil, together with readjustment of the eddy current compensation circuit, provided us the recovery time to the static field less than 1 msec, making it possible to measure the diffusion coefficient of Na+ ion using the pulsed field gradient spin echo method. The diffusion coefficient of Na+ ion in skeletal muscle isolated from bullfrog was 0.6 time larger than that in Ringer's solution, suggesting a high viscosity in the skeletal muscle. (Keyword) nmr probe / diffusion / Naイオン / muscle (Tokushima University Institutional Repository) ● Metadata: 109616 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050583647831092096 (Tokushima University Institutional Repository: 109616, CiNii: 1050583647831092096)
5.	Kazuyoshi Kitaoka, Mitsuo Kitamura, Shun Aoi, Noriyuki Shimizu and Kazuo Yoshizaki : Chronic exposure to an extremely low-frequency magnetic field induces depression-like behavior and corticosterone secretion without enhancement of the hypothalamicpituitaryadrenal axis in mice, Bioelectromagnetics, Vol.34, No.1, 43-51, 2013. (Summary) An extremely low-frequency magnetic field (ELF-MF) is generated by power lines and household electrical devices. Many studies have suggested an association between chronic ELF-MF exposure and anxiety and/or depression. The mechanism of these effects is assumed to be a stress response induced by ELF-MF exposure. However, this mechanism remains controversial. In the present study, we investigated whether chronic ELF-MF exposure (intensity, 1.5 mT; [corrected] total exposure, 200 h) affected emotional behavior and corticosterone synthesis in mice. ELF-MF-treated mice showed a significant increase in total immobility time in a forced swim test and showed latency to enter the light box in a light-dark transition test, compared with sham-treated (control) mice. Corticosterone secretion was significantly high in the ELF-MF-exposed mice; however, no changes were observed in the amount of the adrenocorticotropic hormone and the expression of genes related to stress response. Quantification of the mRNA levels of adrenal corticosteroid synthesis enzymes revealed a significant reduction in Cyp17a1 mRNA in the ELF-MF-exposed mice. Our findings suggest the possibility that high intensity and chronic exposure to ELF-MF induces an increase in corticosterone secretion, along with depression- and/or anxiety-like behavior, without enhancement of the hypothalamic-pituitary-adrenal axis. (Keyword) Adrenal Glands / Adrenocorticotropic Hormone / Animals / Behavior, Animal / Body Size / Corticosterone / Depression / Electromagnetic Fields / Emotions / Gene Expression Regulation, Enzymologic / Hypothalamo-Hypophyseal System / Male / Mice / Mice, Inbred ICR / Norepinephrine / Time Factors / Tyrosine 3-Monooxygenase (Link to Publication Site) ● Publication site (DOI): 10.1002/bem.21743 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22753092 ● Search Scopus @ Elsevier (PMID): 22753092 ● Search Scopus @ Elsevier (DOI): 10.1002/bem.21743 (DOI: 10.1002/bem.21743, PubMed: 22753092)
6.	Masayuki Shono, Mitsuo Kitamura, Ken Konaka, Kazuyoshi Kawazoe and Kazuo Minakuchi : Effects of mechanical pressure on cytoskeleton in human umbilical vein endothelial cells cultured in a newly developed chamber., Journal of Medical Technology, Vol.56, No.7, 803-805, 2012. (Keyword) 血管内皮細胞 / 加圧チャンバー / DAPI染色 / アクチン (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390002209906676352 (CiNii: 1390002209906676352)
7.	Masayuki Shono, Mitsuo Kitamura, 小中健, Kazuyoshi Kawazoe and Kazuo Minakuchi : Changes in DNA structure in the DPI-induced apoptosis in cultured human HepG2 cells, Journal of Medical Technology, Vol.56, No.3, 331-334, 2012. (Keyword) DPI / HepG2 / apoptosis / DAPI / PI (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390565159860698496 (CiNii: 1390565159860698496)
8.	Hirotaka Nishisako, Toshitaka Ikehara, Tairoh Shiraishi, Yuki Minami, Ichinose Hiromi Sasaki, Hitoshi Houchi, Kazuyoshi Kawazoe, Kazuo Minakuchi, Mitsuo Kitamura, Masayuki Shono, Kazuo Yoshizaki, Yohsuke Kinouchi and Hiroshi Miyamoto : Effects of a 1.5 T time-varying magnetic field on cell volume regulation of bovine adrenal chromaffin cells in hyposmotic media., The Journal of Medical Investigation : JMI, Vol.58, No.1-2, 95-105, 2011. (Summary) Effects of a time-varying magnetic field on cell volume regulation by hyposmotic stress in cultured bovine adrenal chromaffin cells were examined. Through regulatory volume decrease (RVD), cell volume of chromaffin cells that were incubated in a hypotonic medium initially increased, reached a peak and finally recovered to the initial value. Two hour exposure to a magnetic field and addition of cytochalasin D increased peak value and delayed return to initial value. Intracellular F-actin contents initially decreased but returned to normal levels after 10 sec. Two hour exposure to the magnetic field and addition of cytochalasin D continuously reduced the F-actin content. Results suggest that exposure to the magnetic field stimulated disruption of the actin cytoskeleton and that the disruption delayed the recovery to the volume prior to osmotic stress. (Keyword) Actins / Animals / calcium / Cattle / Cell Size / Cells, Cultured / Chromaffin Cells / Cytochalasin D / Hypotonic Solutions / Magnetics / Osmotic Pressure / Time Factors (Tokushima University Institutional Repository) ● Metadata: 111307 (Link to Publication Site) ● Publication site (DOI): 10.2152/jmi.58.95 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21372493 ● Search Scopus @ Elsevier (PMID): 21372493 ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.58.95 (Tokushima University Institutional Repository: 111307, DOI: 10.2152/jmi.58.95, PubMed: 21372493)
9.	Kazuyoshi Kitaoka, Hajime Miura, Mitsuo Kitamura, Masatake Akutagawa, Yohsuke Kinouchi and Kazuo Yoshizaki : Feed-forward changes in carotid blood flow velocity during active standing., Neuroscience Letters, Vol.487, No.2, 240-245, 2011. (Summary) Orthostatic changes induce temporary loss of circulatory regulation. Feedback systems react to cardiovascular alterations to compensate for the instability. To clarify the existence of anticipatory cardiovascular regulation during active standing, we continuously recorded blood flow velocity (BFV) in the common carotid artery and cerebral blood volume (CBV) in healthy men. The maximum BFV value decreased significantly before standing in the reaction-time condition. The decrease significantly correlated with the change in systolic blood pressure that accompanies upright standing from a supine position. The anticipatory BFV decrease disappeared during self-paced standing, and all BFV parameters significantly declined after the self-paced standing. The CBV recording showed a significant increase in oxyhemoglobin levels before standing in the reaction-time condition. Our study suggests that some feed-forward cardiovascular regulation triggered by central command could be activated before standing, and it may play a functional role in the maintenance of cerebral perfusion during standing. (Keyword) Adult / Blood Flow Velocity / Blood Pressure / Carotid Artery, Common / Humans / Hypotension, Orthostatic / Male / Middle Aged / Posture / Reaction Time / Young Adult (Link to Publication Site) ● Publication site (DOI): 10.1016/j.neulet.2010.10.030 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20970481 ● Search Scopus @ Elsevier (PMID): 20970481 ● Search Scopus @ Elsevier (DOI): 10.1016/j.neulet.2010.10.030 (DOI: 10.1016/j.neulet.2010.10.030, PubMed: 20970481)
10.	Shichijo Hiroe, Keiko Hosokawa, Hisao Yamaguchi, Toshitaka Ikehara, Mitsuo Kitamura, Kazuo Yoshizaki, Yohsuke Kinouchi, Hiroshi Miyamoto and Aizawa Katsuo : Effects of ELF magnetic fields on signal transduction in osteoblast-like cells, 日本生体磁気学会誌特別号第20回日本生体磁気学会論文集, Vol.18, No.1, 166-167, 2005.
11.	Toshitaka Ikehara, Hisao Yamaguchi, Keiko Hosokawa, Hitoshi Houchi, Ki Ho Park, Kazuo Minakuchi, Hideki Kashimoto, Mitsuo Kitamura, Yohsuke Kinouchi, Kazuo Yoshizaki and Hiroshi Miyamoto : Effects of a time-varying strong magnetic field on transient increase in Ca²⁺ release induced by cytosolic Ca²⁺ in cultured pheochromocytoma cells, Biochimica et Biophysica Acta (BBA) - General Subjects, Vol.1724, No.1-2, 8-16, 2005. (Summary) Exposure of pheochromocytoma (PC 12) cells to a time-varying 1.51 T magnetic field inhibited an increase in the intracellular Ca2+ concentration ([Ca2+]i) induced by addition of caffeine to Ca(2+)-free medium. This inhibition occurred after a 15-min exposure and was maintained for at least 2 h. [Ca2+]i sharply increased in cells loaded with cyclic ADP-ribose, and 2-h exposure significantly suppressed the increase. Addition of ATP induced a transient increase in intracellular Ca2+ release mediated by IP3 receptor, and this increase was strongly inhibited by the exposure. Results indicated that the magnetic field exposure strongly inhibited Ca2+ release mediated by both IP3 and ryanodine receptors in PC 12 cells. However, thapsigargin-induced Ca2+ influx (capacitative Ca2+ entry) across the cell membrane was unaffected. The ATP content was maintained at the normal level during the 2-h exposure, suggesting that ATP hydrolysis was unchanged. Therefore, Mg2+ which is known to be released by ATP hydrolysis and inhibit intracellular Ca2+ release may not relate the exposure-caused inhibition. Eddy currents induced in culture medium appear to change cell membrane properties and indirectly inhibit Ca2+ release from endoplasmic reticulum and other Ca2+ stores in PC 12 cells. (Keyword) ryanodine receptor / caffeine / cyclic ADP-ribose / time-varying magnetic field / eddy current (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbagen.2005.03.008 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15890451 ● Summary page in Scopus @ Elsevier: 2-s2.0-20444422530 (DOI: 10.1016/j.bbagen.2005.03.008, PubMed: 15890451, Elsevier: Scopus)
12.	Toshitaka Ikehara, Ki Ho Park, Hisao Yamaguchi, Keiko Hosokawa, Hitoshi Houchi, Mami Azuma, Kazuo Minakuchi, Hideki Kashimoto, Mitsuo Kitamura, Yohsuke Kinouchi, Kazuo Yoshizaki and Hiroshi Miyamoto : Effects of Time-Varying Strong Magnetic Fields on Rerease of Cytosolic Free Ca²⁺ from Intracellular Stores in Cultured Bovine Adrenal Chromaffin Cells, Bioelectromagnetics, Vol.23, No.7, 505-515, 2002. (Summary) This study was made to explain the mechanisms for the effects of exposure to a time varying 1.51 T magnetic field on the intracellular Ca(2+) signaling pathway. The exposure inhibited an increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) in bovine chromaffin cells induced by addition of bradykinin (BK) to a Ca(2+) free medium. The exposure did not change BK induced production of inositol 1,4,5-trisphosphate (IP(3)). [Ca(2+)](i) was markedly increased in IP(3) loaded cells, and this increase was inhibited by the magnetic field exposure. A similar increase in [Ca(2+)](i) by other drugs, which stimulated Ca(2+) release from intracellular Ca(2+) stores, was again inhibited by the same exposure. However, transmembrane Ca(2+) fluxes caused in the presence of thapsigargin were not inhibited by the magnetic field exposure in a Ca(2+) containing medium. Inhibition of the BK induced increase in [Ca(2+)](i) by the exposure for 30 min was mostly recovered 1 h after exposure ended. Our results reveal that the magnetic field exposure inhibits Ca(2+) release from intracellular Ca(2+) stores, but that BK bindings to BK receptors of the cell membrane and intracellular inositol IP(3) production are not influenced. (Keyword) magnetic field / cell physiology (Link to Publication Site) ● Publication site (DOI): 10.1002/bem.10043 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12224054 ● Summary page in Scopus @ Elsevier: 2-s2.0-0036778437 (DOI: 10.1002/bem.10043, PubMed: 12224054, Elsevier: Scopus)

Academic Letter:

1.	七條裕衣, Keiko Hosokawa, Hisao Yamaguchi, Toshitaka Ikehara, Mitsuo Kitamura, Masayuki Shono, Kazuo Yoshizaki, Yohsuke Kinouchi, Hiroshi Miyamoto and 會沢勝夫 : ImSpecter法による培養骨牙細胞に及ぼすELF変動磁界の影響, Technical Report of IEICE, MBE, Vol.32, 49-52, 2004. (Keyword) ImSpecter法 / 培養骨牙細胞 / ELF変動磁界

Proceeding of International Conference:

1.	Toshitaka Ikehara, Shiraishi Tairho, Nishisako Hirotaka, Hisao Yamaguchi, Mitsuo Kitamura, Masayuki Shono, Kazuo Yoshizaki, Kazuyoshi Kawazoe, Kazuo Minakuchi, Yohsuke Kinouchi and Hiroshi Miyamoto : EFFECTS OF TIME-VARYING MAGNETIC FIELD ON REGULATORY VOLUME DECREASE IN BOVINE ADRENAL CHROMAFFIN CELLS, The Bioelectromagnetics Society 30th Annual Meeting, 368-369, San Diego, Jan. 2008.
2.	Toshitaka Ikehara, Nishisako Hirotaka, Shiraishi Tairoh, Hisao Yamaguchi, Masayuki Shono, Mitsuo Kitamura, Kazuyoshi Kawazoe, Kazuo Minakuchi, Kazuo Yoshizaki, Yohsuke Kinouchi and Hiroshi Miyamoto : Effects of a time-varying magnetic field on bovine adrenal chromaffin cells., Proceedings of the International Symposium on Biological and Physiological Engineering /The 22nd SICE Symposium on Biological and Physiological Engineering, 117-118, Harbin, Jan. 2008.
3.	Toshitaka Ikehara, Yuki Minami, Shiota Naoko, Hisao Yamaguchi, Masayuki Shono, Mitsuo Kitamura, Kazuyoshi Kawazoe, Kazuo Minakuchi, Kazuo Yoshizaki, Yohsuke Kinouchi and Hiroshi Miyamoto : EFFECTS OF A TIME-VARYING MAGNETIC FIELD ON CELL VOLUME REGULATION OF CULTURED BOVINE ADRENAL CHROMAFFIN CELLS, Abstract Book The Bioelectromaginetics Society 29th Annual Meeting, 375-376, Kanazawa, Jun. 2007.
4.	Toshitaka Ikehara, Hiromi Sasaki, Yuki Minami, Hisao Yamaguchi, Keiko Hosokawa, Kazuyoshi Kawazoe, Mitsuo Kitamura, Masayuki Shono, Kazuo Yoshizaki, Yohsuke Kinouchi and Hiroshi Miyamoto : Effects of a time-varying magnetic field on intracellular organells and actin filaments of bovine adrenal chronmaffin cells, Abstract Book The Bioelectromaginetics Society 28th Annual Meeting, 81-82, Cancun, Jun. 2006.
5.	Toshitaka Ikehara, Hiromi Sasaki, Tadahiro Teramoto, Hitoshi Houchi, Keiko Hosokawa, Hisao Yamaguchi, Mitsuo Kitamura, Masayuki Shono, Kazuo Yoshizaki, Yohsuke Kinouchi and Hiroshi Miyamoto : Effects of a time-varying magnetic field on intracellular Ca²⁺ movements of cultured bovine adrenal chromaffin cells, Bioelectromagnetics 2005 Abstract Collection, 405-406, Dublin, Jun. 2005. (Keyword) magnetic field / Ca2+ / Endoplasmic reticulum / actin / chromaffin cells
6.	Hisao Yamaguchi, Keiko Hosokawa, Hiroe Shichijo, Mitsuo Kitamura, Akira Soda, Toshitaka Ikehara, Yohsuke Kinouchi, Kazuo Yoshizaki, Hiroshi Miyamoto and Katsuo Aizawa : Effects of ELF magnetic field on signal transduction for the deffrentiation of osteoblast-like signal transduction for the deffrentiation of osteoblast-like cells, Bioelectromagnetics 2005 Abstract Collection, 348, Dublin, Jun. 2005.
7.	Toshitaka Ikehara, Tadahiro Teramoto, Hitoshi Houchi, Keiko Hosokawa, Hisao Yamaguchi, Yohsuke Kinouchi, Mitsuo Kitamura, Masayuki Shono, Kazuo Yoshizaki and Hiroshi Miyamoto : Effects of a switched 1.5 tesla magnetic field on intracellular Ca²⁺ signaling pathways of adrenal chromaffin cells, Proceedings of 26th BEMS Meeting, Washington, D.C., Jun. 2004.
8.	Hisao Yamaguchi, Keiko Hosokawa, Hiroe Shichijo, Mitsuo Kitamura, Akira Soda, Toshitaka Ikehara, Yohsuke Kinouchi, Kazuo Yoshizaki, Hiroshi Miyamoto and Katsuo Aizawa : Effects of ELF magnetic fields on signal for differentiation of cultured osteoblastic cells by multispectral imaging system., Proceedings of 26th BEMS Meeting, 216, Washington, D.C., Jun. 2004.
9.	Yoshiko Nakano, Keiko Hosokawa, Hisao Yamaguchi, Park Ho Ki, Toshitaka Ikehara, Mitsuo Kitamura, Yohsuke Kinouchi, Kazuo Yoshizaki and Hiroshi Miyamoto : Design of an Uniform Magnetic Field Exposure System for Experiments of Biological Effects of Magnetic Fields on Culture Cells, The Japanese Society of Magnetic Applications in Dentistry (JSMAD)The Second International Conference on Magnetic Applications in Dentistry, Feb. 2003.
10.	Toshitaka Ikehara, Ki Ho Park, Keiko Hosokawa, Mitsuo Kitamura, Yohsuke Kinouchi, Kazuo Yoshizaki, Hisao Yamaguchi and Hiroshi Miyamoto : Effects of Time-Varying Magnetic Fields on Increase in Intracellular Free Ca²⁺ in Cultured Cells, Proc. of International Symposium on Bioelectromagnetics, 134-137, Kyoto, Oct. 2002. (Keyword) Magnetic Field / cell
11.	Toshitaka Ikehara, Keiko Hosokawa, Hitoshi Houchi, Mitsuo Kitamura, Yohsuke Kinouchi, Hiroshi Miyamoto, Ryohei Murato, Ki Ho Park, Hisao Yamaguchi and Kazuo Yoshizaki : Effects of magnetic fields on intracellular Ca²⁺ mobilization and differentiation of neural cells, XXXIV International Congress of Physiological Sciences Abstract Book, a1599, Christchurch, New Zealand, Aug. 2001.

Proceeding of Domestic Conference:

1.	Kazuyoshi Kitaoka, Mitsuo Kitamura, 吉田朋広, 角陸文哉 and 川田知代 : 極低周波変動磁界の曝露はcAMP濃度の上昇を介して副腎皮質由来細胞株のステロイド分泌量を亢進させる, 第29回日本生体磁気学会大会, May 2014.
2.	Shiyori Kawata, FUmiya Kadoriku, Mitsuo Kitamura and Kazuyoshi Kitaoka : Exploration of the mechanism for extremely low-frequency magnetic field-indu ced adrenal steroids up-regulation in Y-1 cell, The 91st Annual Meeting of the Physiological Society of Japan, Mar. 2014.
3.	Fumiya Kadoriku, Shiyori Kawata, Mitsuo Kitamura and Kazuyoshi Kitaoka : Chronic exposure of extremely low-frequency magnetic field stimulates aldost erone secretion in H295R cell, The 91st Annual Meeting of the Physiological Society of Japan, Mar. 2014.
4.	早野尚志, Mitsuo Kitamura and Kazuo Yoshizaki : --, 第41回日本磁気共鳴医学会大会, Sep. 2013.
5.	Tomohiro Yoshida, Shun Aoi, Kazuyoshi Kitaoka, Mitsuo Kitamura, Noriyuki Shimizu and Kazuo Yoshizaki : Chronic exposure to extremely low-frequency magnetic field stimulates corticosteroid secretion in both mice and Y-1 cell line, The 90th annual meeting of Physiological Society of Japan, Mar. 2013.
6.	Masayuki Shono, Mitsuo Kitamura, 小中健, Kazuyoshi Kawazoe and Kazuo Minakuchi : 開発培養チャンバーを用いた物理的圧迫によるHUVEC細胞骨格変化の影響, 第246回徳島医学会学術集会, Feb. 2013.
7.	Kazuyoshi Kitaoka, Mitsuo Kitamura, Shun Aoi, Tomohiro Yoshida, Noriyuki Shimizu and Kazuo Yoshizaki : 極低周波変動磁界慢性暴露による副腎皮質ホルモンの分泌増強作用, 第64回日本生理学会中国四国地方会, Oct. 2012.
8.	Kazuyoshi Kitaoka, Mitsuo Kitamura and Kazuo Yoshizaki : スポーツクライマーおよび一般成人における両腕懸垂時の血中乳酸濃度, 第67回日本体力医学会大会, Sep. 2012.
9.	Shun Aoi, Kazuyoshi Kitaoka, Mitsuo Kitamura, Noriyuki Shimizu and Kazuo Yoshizaki : 極低周波変動磁界の慢性暴露はマウスにおいて副腎皮質ホルモン分泌を増強させる, 第27回日本生体磁気学会大会, May 2012.
10.	Kazuyoshi Kitaoka, Mitsuo Kitamura and Kazuo Yoshizaki : スポーツクライマーおよび一般成人における両腕懸垂時の乳酸閾値, 第69回日本体力医学会中国・四国地方会, May 2012.
11.	早野尚志, Mitsuo Kitamura and Kazuo Yoshizaki : 拡散測定のための生体試料測定用NMRプローブの作製と評価, Shikoku Acta Medica, Vol.67, No.5,6, 288, Dec. 2011.
12.	早野尚志, Mitsuo Kitamura and Kazuo Yoshizaki : 拡散測定のための生体試料測定用NMRプローブの作製と評価, 第243回徳島医学会学術集会, Jul. 2011.
13.	早野尚志, Mitsuo Kitamura, 浜岡建城 and Kazuo Yoshizaki : NMR検出コイルの作製と生物試料測定への応用, 第62回日本生理学会中国四国地方会, Nov. 2010.
14.	Kazuyoshi Kitaoka, Hajime Miura, Mitsuo Kitamura, Masatake Akutagawa, Yohsuke Kinouchi and Kazuo Yoshizaki : Anticipatory changes in carotid blood flow velocity and cerebral blood flow preceds standing movement., 第87回日本生理学会大会, May 2010.
15.	Toshitaka Ikehara, 西迫寛隆, 白石泰郎, Kazuyoshi Kawazoe, Kazuo Minakuchi, Mitsuo Kitamura, 庄野正行, Kazuo Yoshizaki, Yohsuke Kinouchi and Hiroshi Miyamoto : Effects of time-varying magnetic field on cell volume regulation of bovine adrenal chromaffin cells in hyposmotic medium., 第87回日本生理学会大会, May 2010.
16.	Toshitaka Ikehara, Hirotaka Nishisako, Tairoh Shiraishi, Kazuyoshi Kawazoe, Kazuo Minakuchi, Mitsuo Kitamura, Masayuki Shono, Kazuo Yoshizaki, Yohsuke Kinouchi and Hiroshi Miyamoto : Effects of time-varying magnetic field on cell volume regulation of bovine adrenal chromaffin cells in hyposmotic medium, The Journal of Physiological Sciences, Vol.60, No.Supplement 1, S106, 2010.
17.	Kazuyoshi Kitaoka, Hajime Miura, Mitsuo Kitamura, Masatake Akutagawa, Yohsuke Kinouchi and Kazuo Yoshizaki : Anticipatory changes in carotid blood flow velocity and cerebral blood flow precede standing movement, The Journal of Physiological Sciences, Vol.60, No.Supplement 1, S92, 2010.
18.	Kazuyoshi Kitaoka, Hajime Miura, Mitsuo Kitamura, Akihiro Inagaki, Masatake Akutagawa, Yohsuke Kinouchi and Kazuo Yoshizaki : 起立動作時における予測的な脳血流量，総頸動脈血流速度の変化, Journal of Physiological Society of Japan, Vol.72, No.4, 126-127, 2010.
19.	Kazuo Yoshizaki, Hayano Takashi, Mitsuo Kitamura and Arizono Naoki : Histamine and exocytosis in mast cells studied with 1H-NMR, 生物物理第47回年会講演予稿集, Vol.49, S56, Oct. 2009.
20.	早野尚志, Mitsuo Kitamura, 浜岡建城 and Kazuo Yoshizaki : 1H-NMRによる拡散画像測定法の開発, Journal of Physiological Society of Japan, Vol.70, No.2, 66, 2008.
21.	Hisao Yamaguchi, Keiko Hosokawa, Toshitaka Ikehara, Hiroe Shichijo, Mitsuo Kitamura, Kazuo Yoshizaki, Yohsuke Kinouchi, Hiroshi Miyamoto and Katsuo Aizawa : Effects of ELF differetiation of cultured osteoblast-like cells, The Journal of Physiological Sciences, Vol.56, S111, Mar. 2006. (Keyword) osteoblast
22.	Toshitaka Ikehara, Hiromi Sasaki, Yuki Minami, Keiko Hosokawa, Hisao Yamaguchi, Mitsuo Kitamura, Masayuki Shono, Kazuyoshi Kawazoe, Kazuo Yoshizaki, Yohsuke Kinouchi and Hiroshi Miyamoto : Effects of a time-varying magnetic field on intracellular organells of bovine adrenal chronmaffin cells in culture, The Journal of Physiological Sciences, Vol.56, S110, Mar. 2006. (Keyword) intracellular Ca2+ / acetylcholine / time-varying magnetic field / organells
23.	Hiromi Sasaki, Tadahiro Teramoto, Toshitaka Ikehara, Hisao Yamaguchi, Keiko Hosokawa, Hitoshi Houchi, Mitsuo Kitamura, Masayuki Shono, Kazuo Yoshizaki, Yohsuke Kinouchi and Hiroshi Miyamoto : Effects on intracellular signal transduction of adrenal chromaffin cells of exposure to a switched 1.5 T magnetic field, 日本生体磁気学会雑誌, Vol.18, No.1, 158, Jul. 2005.
24.	Keiko Hosokawa, Hisao Yamaguchi, Toshitaka Ikehara, Yui Shichijo, Mitsuo Kitamura, Kazuo Yoshizaki, Yohsuke Kinouchi, Hiroshi Miyamoto and Katsuo Aizawa : Effects of ELF magnetic fields on signal transduction for the differentiation of osteoblast-like cells, The Japanese Journal of Physiology, May 2005.
25.	Toshitaka Ikehara, Hiromi Sasaki, Tadahiro Teramoto, Keiko Hosokawa, Mitsuo Kitamura, Masayuki Shono, Kazuo Yoshizaki, Yohsuke Kinouchi, Hisao Yamaguchi and Hiroshi Miyamoto : Effects of a time-varying magnetic field on transient increase in intracellular Ca²⁺ concentration of bovine adrenal chromaffin cells, The Japanese Journal of Physiology, May 2005.
26.	Toshitaka Ikehara, Tadahiro Eramoto, Hiromi Sasaki, Hisao Yamaguchi, Keiko Hosokawa, Mitsuo Kitamura, Masayuki Shono, Hitoshi Houchi, Kazuo Yoshizaki, Yohsuke Kinouchi and Hiroshi Miyamoto : Effect of time-varying strong magnetic field on transient increase in intracellular Ca2+ in bovine adrenal chromaffin cells, 第8回シンポジウムおよび特定領域研究「強磁場新機能」第2回シンポジウム, Sep. 2004. (Keyword) trong magnetic / intracellular Ca2+ / bovine adrenal chromaffin cells
27.	Shichijo Hiroe, Keiko Hosokawa, Hisao Yamaguchi, Toshitaka Ikehara, Mitsuo Kitamura, Shono Masayuki, Kazuo Yoshizaki, Yohsuke Kinouchi, Hiroshi Miyamoto and Aizawa Katsuo : 培養骨芽細胞に及ぼすELF変動磁界の影響, Journal of Shikoku-Section Joint Convention of the Institutes of Electrical and Related Engineers, Sep. 2004.
28.	Keiko Hosokawa, Hisao Yamaguchi, Toshitaka Ikehara, 七条裕衣, Mitsuo Kitamura, Kazuo Yoshizaki, Yohsuke Kinouchi, Hiroshi Miyamoto and 會沢勝夫 : Imspector法による培養骨芽細胞の分化に及ぼすELF磁界の影響, 第81回日本生理学会大会, Jun. 2004.
29.	Toshitaka Ikehara, Ki Ho Park, Hitoshi Houchi, Hisao Yamaguchi, Keiko Hosokawa, Mitsuo Kitamura, Masayuki Shono, Yohsuke Kinouchi, Kazuo Yoshizaki and Hiroshi Miyamoto : Tesla変動強磁界が牛副腎髄質クロマフィン細胞に及ぼす影響., 第18回日本生体磁気学会発表, 2003. (Keyword) 変動強磁界 / 牛副腎髄質クロマフィン細胞
30.	Hisao Yamaguchi, 中野芳子, Keiko Hosokawa, Toshitaka Ikehara, Mitsuo Kitamura, Yohsuke Kinouchi, Kazuo Yoshizaki and Hiroshi Miyamoto : Effects of ELF magnetic fields on differentiation of cultured osteoblasts like cells., The Journal of Japan Biomagnetism and Bioelectromagnetics Society, Vol.16, No.1, 82-83, May 2003.
31.	Toshitaka Ikehara, Ki Ho Park, Hitoshi Houchi, Hisao Yamaguchi, Keiko Hosokawa, Mitsuo Kitamura, Masayuki Shono, Yohsuke Kinouchi, Kazuo Yoshizaki and Hiroshi Miyamoto : Effects on bovine adrenal chromaffin cells to a switched 1.5Tesla magnetic field, The Journal of Japan Biomagnetism and Bioelectromagnetics Society, Vol.16, No.1, 80-81, May 2003.
32.	Keiko Hosokawa, Hisao Yamaguchi, Toshitaka Ikehara, 中野芳子, Mitsuo Kitamura, Kazuo Yoshizaki, Yohsuke Kinouchi and Hiroshi Miyamoto : 骨芽細胞の増殖と分化に及ぼすELF磁界の影響, 第80回日本生理学会大会, Mar. 2003.
33.	Keiko Hosokawa, 中野芳子, Hisao Yamaguchi, Toshitaka Ikehara, Mitsuo Kitamura, Yohsuke Kinouchi, Kazuo Yoshizaki and Hiroshi Miyamoto : ELF 変動磁界が培養骨芽細胞の分化誘導に及ぼす影響, Journal of Physiological Society of Japan, Vol.64, No.12, 295, 2002.
34.	中野芳子, Keiko Hosokawa, Hisao Yamaguchi, Toshitaka Ikehara, Mitsuo Kitamura, Yohsuke Kinouchi, Kazuo Yoshizaki and Hiroshi Miyamoto : 培養骨芽細胞の分化誘導に及ぼすELF 変動磁界の影響, 日本生理学会雑誌, Vol.64, No.4, 90, 2002.
35.	Keiko Hosokawa, Hisao Yamaguchi, Toshitaka Ikehara, Mitsuo Kitamura, Kazuo Yoshizaki and Hiroshi Miyamoto : 骨芽細胞の増殖と分化に及ぼすELF磁界の影響, 日本生理学会雑誌, Vol.63, No.4, 137, 2001.

Et cetera, Workshop:

1.	Kazuyoshi Kitaoka, Shun Aoi, Mitsuo Kitamura, Masatake Akutagawa, Yohsuke Kinouchi and Kazuo Yoshizaki : 起立に先行する頸動脈血流の変化と血圧との関連, 第63回生理学会中国四国地方会, Oct. 2011.
2.	Kazuyoshi Kitaoka, Shun Aoi, Mitsuo Kitamura, Masatake Akutagawa, Yohsuke Kinouchi and Kazuo Yoshizaki : 起立に伴う予測的頸動脈血流速度変化の検討-血流速度と血圧の同時記録による解析-, 第67回日本体力医学会中国・四国地方会大会, May 2011.
3.	Kazuyoshi Kitaoka, Hajime Miura, Mitsuo Kitamura, Masatake Akutagawa, Yohsuke Kinouchi and Kazuo Yoshizaki : 起立に先行する総頸動脈血流速度の予測的変化, 第66回日本体力医学会中国・四国地方会大会, Nov. 2010.
4.	早野尚志, Mitsuo Kitamura and Kazuo Yoshizaki : 1H-NMRスペクトルによるマスト細胞のヒスタミン分泌の解析, Shikoku Acta Medica, Vol.65, No.5,6, 206, Dec. 2009.
5.	早野尚志, Mitsuo Kitamura, 浜岡建城 and Kazuo Yoshizaki : 1H-MRI顕微画像測定法の基礎的検討, Shikoku Acta Medica, Vol.63, No.5,6, 252-253, Dec. 2007.
6.	Mitsuo Kitamura, Akihiro Inagaki and Kazuo Yoshizaki : 生体試料測定のためのNMR装置特性の基礎的検討, Shikoku Acta Medica, Vol.61, No.1,2, 60, Apr. 2005.
7.	Hiroe Shichijo, Keiko Hosokawa, Hisao Yamaguchi, Toshitaka Ikehara, Mitsuo Kitamura, Masayuki Shono, Kazuo Yoshizaki, Yohsuke Kinouchi, Hiroshi Miyamoto and Katsuo Aizawa : Effects of ELF Magnetic Fields on Cultured Osteoblast Cells by ImSpector System, IEICE Technical Report, Vol.104, No.179, 49-52, Jul. 2004. (Summary) It is reported that the magnetic field exposure is for fracture healing of the intractable, and it is shown experimentally that the biofunction in which the calcium ion is concerned is affected. In this study, the effects of sine wave alternating current magnetic field in the ELF region on the osteoblast was probed using the spectral diffraction image microscope system, and the basic action mechanism was examined. Using insulin-like growth factor (IGF-1) which is the factor shoulders the important role for growth and differentiation of the cells, the difference between the effect by exposure was examined with these drug effects to the osteoblasts. The effect which the cell receives in cencer and periferal regions of the Schale in which the induced current density differs was compared in order to examine the effect of the induced current which arises in the Shale by fluctuation magnetic fields.

Report:

1.	Mitsuo Kitamura : 細胞周期解析の実際 PI (Propidium Iodide) 染色測定での注意点, 徳島大学技術支援部技術報告, No.6, 1-3, Feb. 2023. (Summary) フローサイトメーターを用いPI(Propidium Iodide)染色を行った細胞の細胞周期解析方法と注意点をまとめたので報告する． (Keyword) Flow cytometry / cell cycle
2.	Mitsuo Kitamura : Simultaneous measurement of cardiac action potential and heart contractivity in bullfrog heart., 生理学技術研究会報告, No.36, 98-99, 岡崎市, Jul. 2014.
3.	Mitsuo Kitamura : 平成22年度中国・四国地区国立大学法人等技術職員研修分野別実習報告, 徳島大学大学院HBS研究部教育・研究技術支援部報告書, 58-59, Mar. 2014.
4.	Mitsuo Kitamura : 生理機能学分野における業務について, 徳島大学大学院HBS研究部教育・研究技術支援部報告書, 36-37, Mar. 2014.
5.	Mitsuo Kitamura : Analysis of spectral diffraction image by the imaging spectroscope, 生理学技術研究会報告, No.27, 142-143, 岡崎市, May 2005.
6.	Mitsuo Kitamura : Management changing, installation and operation report of NMR device, 生理学技術研究会報告, No.25, 110-111, 岡崎市, Jul. 2003.
7.	Mitsuo Kitamura : The data acquisition from analytical instrument using the A/D board, 生理学技術研究会報告, No.24, 132-133, 岡崎市, Sep. 2002.
8.	Mitsuo Kitamura : Trial manufacture of a simple pules generator, 生理学技術研究会報告, No.23, 209-210, 岡崎市, Oct. 2001.

技術部門長 : Kitamura, Mitsuo

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